cytellin and sesamin

Leishmaniasis is a neglected tropical disease caused by Leishmania parasitic protozoa, which currently lacks efficient treatment. Natural products have shown promise as a potential source for antiprotozoal drugs. This work focuses on the antileishmanial potential of Sassafras albidum (Lauraceae) bark extract. The crude bark extract of S. albidum showed excellent antileishmanial activity with an IC50 value less than 12.5 μg/mL against promastigotes of L. amazonensis. The chloroform stem bark extract of S. albidum was subjected to preparative column chromatography. Five compounds were isolated, purified by recrystallization, and identified as sesamin, spinescin, β-sitosterol, hexatriacontanal, and 1-triacontanol. Antileishmanial and cytotoxic screening were performed on these compounds. Sesamin exhibited the best activity against L. amazonensis with an IC50 of 15.8 μg/mL and was not cytotoxic to mouse macrophage cells (CC50 > 100 μg/mL).

Topics: Dioxoles; Fatty Alcohols; Leishmania; Lignans; Microbial Sensitivity Tests; Plant Extracts; Sassafras; Sitosterols; Trypanocidal Agents

To study the chemical constituents of Pattra Medicine Euodia lepta in Xishuangbanna of Yunnan Province.. The chemical constituents were isolated and purified by chromatographic techniques, and identified by NMR, MS and other spectral methods.. In 60% ethanol extract from the stems,and 95% ethanol extract from the leaves, six compounds and two compounds were isolated and identified as pachypodol( 1) ,3-(3-methyl-but-2-enyl )umbelliferone(2),7-demethylsuberosin (3),beta-sitosterol (4),3,7-dimethoxy kaempferol(5), euolitrine(6), sesamin(7) and p-O-geranyl coumaric acid(8), respectively.. Compound 7 is obtained from Euodia genus for the first time,and compound 8 is obtained from domestic Euodia lepta for the first time.

Topics: China; Coumaric Acids; Dioxoles; Evodia; Furocoumarins; Lignans; Plant Leaves; Plant Stems; Sitosterols

To study the chemical constituents from the aerial part of Euphorbia chrysocoma.. All compounds were isolated and purified by many methods, including siliga gel and reversed phase RP-18 column chromatographies, preparative thin layer chromatography, Sephadex LH-20, and recrystallization. Their structures were mainly elucidated by ESI-MS and NMR spectra and their physical characters.. Six compounds were isolated from the petroleum ether section from 75% ethanol extraction of the material. Their structures were identified as taraxerol (1), epitaraxerol (2), beta-sistosterol (3), beta-sitostenone (4), jolkinolide E (5), and sesamin (6).. Compounds 1, 2, 4, 5, and 6 are isolated from this plant for the first time.

Topics: Dioxoles; Diterpenes; Euphorbia; Lignans; Molecular Structure; Oleanolic Acid; Plant Components, Aerial; Plants, Medicinal; Sitosterols

The chemical constituents of the herb of Cuscuta chinensis Lam. were investigated. Five compounds were isolated from petroleum ether and chloroform fraction. Their structures were identified as beta-sitosterol, d-sesamin, 9(R)-hydroxy-d-sesamin, d-pinoresinol and daucosterol by chemical and spectroscopical methods. All these compounds were isolated from the stem for the first time.

Topics: Chemical Fractionation; Cuscuta; Dioxoles; Drugs, Chinese Herbal; Furans; Lignans; Sitosterols; Spectrum Analysis