cytellin and piperine

Groundnut stem rot caused by Sclerotium rolfsii is a major constraint as it affects the productivity. Although managing this disease using synthetic fungicides is a more feasible method, environmental pollution and side effects caused by them demand some safe fungicides. Seven phytochemicals piperine, quercetin, reserpine, atropine sulfate, β-sitosterol, ethyl protocatechuate and salicylic acid were initially tested against S. rolfsii under in vitro methods. All the compounds exhibited significant effects on mycelial inhibition (except atropine sulfate), sclerotial development, ooze formation, maturity, sclerotial number and germination of S. rolfsii. The more active compounds, piperine, reserpine and β-sitosterol were then evaluated under glasshouse condition by adopting various application methods (seed treatment, foliar application and micro-injection at 2000 μg/mL) on groundnut plants challenged with and without S. rolfsii. All the treatments effectively reduced the plant mortality when tested every 15 days of infection with S. rolfsii. However, the magnitude of reduction varied among the treatments, with the mortality ranging between 9.37 % and 29.68 % compared to the control (40.68 %). The piperine-microinjected plants recorded minimum mortality (3.12 %). The defense enzymes (PAL and PPO) and key end products such as phenolics (total and individual) were determined in the leaf samples collected after 24, 48 and 72 h of infection with S. rolfsii to understand the systemic resistance induction effect. An increase in PAL and PPO activity was observed in all the samples. While microinjection of β-sitosterol caused a maximum PAL induction, piperine caused a maximum PPO activity. Further, samples of piperine treated group showed higher induction of phenolic acids (86.46 μg g

Topics: Alkaloids; Atropine; Basidiomycota; Benzodioxoles; Fungicides, Industrial; Piperidines; Plant Diseases; Polyunsaturated Alkamides; Reserpine; Sitosterols

The project was intended to the phytochemical characterisation from the rudimentary methanolic extract of Chenopodium ambrosioides Linn., which escorts to the isolation of stigmasterol (1), β-sitosterol (2), octadecanoic acid (3), scopoletin (4) and 1-piperoylpiperidine (5). Literature validates the medicinal authentication of these compounds extorted from other sources, while our previous findings regarding microbial activities of different solvent systems fractions are favouring the presence of medicinally important compounds in this species. Herein, however, we report these natural products for the first time from this species.

Topics: Alkaloids; Benzodioxoles; Chenopodium ambrosioides; Methanol; Phytochemicals; Piperidines; Plant Extracts; Plants, Medicinal; Polyunsaturated Alkamides; Sitosterols; Solvents; Stigmasterol

To study the chemical constituents from herb of Rhodobryum roseum.. The compounds were isolated by column chromatography, and identified by IR, NMR data.. 8 compounds were isolated and identified. They are piperine (1), caffeic acid methyl ester (2), uracil glucoside (3), ursolic acid (4), 5alpha, 8alpha-epidioxy-methylcholesta-6, 22-dien-3beta-ol (5), 5alpha, 8alpha-epidioxy-methylcholesta-6,9(11), 22-trien-3beta-ol (6), beta-sitosterol (7), daucosterol (8).. 7 compounds (1-6,8) were isolated from this plant for the first time.

Topics: Alkaloids; Benzodioxoles; Bryophyta; Caffeic Acids; Piperidines; Plants, Medicinal; Polyunsaturated Alkamides; Sitosterols; Triterpenes; Ursolic Acid