cytellin and euscaphic-acid

A new monoterpene (

Topics: A549 Cells; Benzofurans; Cell Cycle Proteins; Drug Screening Assays, Antitumor; HeLa Cells; Humans; Magnetic Resonance Spectroscopy; Molecular Structure; Plant Roots; Saxifragaceae; Sitosterols; Spectrometry, Mass, Electrospray Ionization; Stigmasterol; Transcription Factors; Triterpenes

To study the chemical constituents of Rubus stans.. The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures were identified on the basis of spectroscopic analysis and physicochemical properties.. Eleven compounds were isolated and purified from the ethanol extract of Rubus stans. They were identified as β-sitosterol( 1),betulinic acid( 2),euscaphic acid( 3),ursolic acid( 4),corosolic acid( 5),kaempferol( 6),quercetin( 7),2α,3β,19α,23-tetrahydroxy-urs-12-en-28-oic acid( 8),β-daucosterol( 9),quercetin-3-O-β-D-glucoside( 10) and kaempferol-3-O-β-D-6-O-( p-hydroxycinnamoyl)-glucopyranoside( 11).. All the compounds are isolated from this plant for the first time.

Topics: Betulinic Acid; Kaempferols; Pentacyclic Triterpenes; Rubus; Sitosterols; Triterpenes; Ursolic Acid

To investigate the chemical constituents of Rubus alceaefolius.. Nine compounds were isolated and purified from the petroleum ether extract of 95% alcohol extract of Rubus alceaefolius by repeated column chromatography on silica, Sephadex LH-20 and structurally identified by spectral analysis.. The compounds were identified as chrysophanol(1), physcion (2), β-sitosterol(3), 3-oxotirucalla-7, 24-dien-21-oic acid(4), myricadiol(5), 19-α-hydroxy-3-acetyl-ursolic acid(6), N-benzoylphenylalaninyl-N-benzoylphenylalaninate(7), aurantiamide acetate(8) and euscaphic acid(9).. Compounds land 4~8 are isolated from this plant for the first time, and compounds 4 - 8 are found in plants of Rubus genus for the first time.

Topics: Dipeptides; Emodin; Phytochemicals; Plant Extracts; Rubus; Sitosterols; Solvents; Triterpenes

To investigate the chemical constituents from the roots of Rubus parvifolius.. The chemical constituents were isolated by silica gel column chromatography, Sephadex LH-20, as well as preparative high performance liquid chromatography. Their structures were identified on the basis of physicochemical properties and NMR analysis.. Six comounds were isolated and identified as 3-O-Acetyl-11α, 12α-epoxy-oleanan-28,β-olide( I ) ,3-O-Acetyl-pomolic acid( II ), Ursolic acid( Ill),Ursolic acid acetate (1V ), Euscaphic acid ( V) and β-Sitosterol ( VI).. Compounds I , II and IV are isolated from Rubus parvifolius for the first time.

Topics: Chromatography, High Pressure Liquid; Magnetic Resonance Spectroscopy; Oleanolic Acid; Plant Roots; Rubus; Sitosterols; Triterpenes; Ursolic Acid

To study the chemical constituents of Rubus parvifoliu.. The constituents were isolated by column chromatography and their structures were elucidated through spectroscopic analysis such as 1H-NMR, 13C-NMR, FT-IR, et al.. Seven compounds were isolated from the roots of Rubus parvifolius L., they were identified as p-sitosterol (I), lauric acid (II), O-nitrophenol (III), beta-daucosterol (IV), euscaphic acid (V), camelliagenin A (VI) and(+) -catech in (VII).. Compounds III and VII are isolated from the plant for the first time.

Topics: Anti-Inflammatory Agents, Non-Steroidal; Catechin; Molecular Structure; Nitrophenols; Organophosphorus Compounds; Plant Roots; Plant Stems; Plants, Medicinal; Rosaceae; Sitosterols; Triterpenes

The purpose of this study was to study the hepatoprotective effects of the most promising extract of the root from Rubus aleaefolius Poir. and to isolate and identify the active components. Various crude forms of Rubus aleaefolius have been evaluated for their effects on CCl(4)-induced acute liver injury in mice vivo experimental model. Treatment groups contained 5 sub-groups that were ethanol crude extract; the high/low dosage ethyl acetate or n-butanol fraction; extracted with ethyl acetate or n-butanol after the residues and major constituent; intragastrically administrated with 35 mg/kg; 35, 4.6 mg/kg; 35, 5.8 mg/kg; 35 mg/kg and 3.5 mg/kg for 7 days. The serum samples were collected for biological analysis and also carried out histopathological studies. The low-dosage ethyl acetate fraction was the most active when the fractions were compared. It was found to decrease AST, ALT; to prevent formation of hepatic MDA, NO and intensify the activity of SOD. The histopathological changes induced by CCl(4) were also significantly reduced. The separation revealed the presence of six constituents by a bioassay-guided fractionation, beta-Sitosterol (1), 1beta-Hydroxyeuscaphic acid (2), Oleanolic acid (3), Myrianthic acid (4), Euscaphic acid (5), and Tomentic acid (6). Among them, compounds 2, 4, 5 in Rubus aleaefolius root is reported here for the first time. 1beta-Hydroxyeuscaphic acid (major constituent) showed a tremendous activity and the results confirm the traditional uses of Rubus aleaefolius in treating hepatitis.

Topics: Alanine Transaminase; Animals; Antioxidants; Aspartate Aminotransferases; Carbon Tetrachloride Poisoning; Chemical and Drug Induced Liver Injury; Liver; Male; Malondialdehyde; Mice; Mice, Inbred ICR; Nitric Oxide; Oleanolic Acid; Phytotherapy; Plant Extracts; Plant Roots; Rosaceae; Sitosterols; Superoxide Dismutase; Triterpenes

To study the chemical constituents from the root of Actinidia chinensis.. The roots of A. chinensis were extracted with 95% EtOH. The EtOH extract was suspended in H2O and extracted with petroleum ether, EtOAc and BuOH successively. The compounds were isolated with column chromatography from the EtOAc fraction, and elucidated on the basis of spectral analysis(MS, IR, 1H-NMR, 13C-NMR).. Seven compounds were isolated from the root of A. chinensis, and the structures were identified as 2alpha-hydroxyoleanolic acid (1), 2alpha-hydroxyursolic acid (2), euscaphic acid (3), 23-hydroxyursolic acid (4), 3beta-O-acetylursolic acid (5), ergosta4, 6, 8, (14), 22-tetraen-3-one (6), beta-steriol (7).. All the compounds were obtained from the root of A. chinensis for the first time.

Topics: Actinidia; Oleanolic Acid; Plant Roots; Plants, Medicinal; Sitosterols; Triterpenes

From the roots of Campylotropis hirtella 6 compounds were isolated. Based on the extensive spectrae analysis their chemical structures were identified as beta-sitosterol, oleanolic acid, ursolic acid, euscaphic acid, tomentic acid and rosamultin.

Topics: Drugs, Chinese Herbal; Fabaceae; Molecular Structure; Oleanolic Acid; Plant Roots; Plants, Medicinal; Sitosterols; Triterpenes; Ursolic Acid