cytellin and ergosterol-5-8-peroxide

To study the chemical constituents of Lighularia altaica.. Various chromatographic techniques were used to isolate and purify the chemical compounds, their structure were elucidated by means of mass, physico-chemical properties and NMR spectral data.. Nine compounds were obtained as: lupeol (1), oleanolic acid (2), 3beta, 16beta-dihydroxy-12-oleanen-28-al (3), ergosterol peroxide (4), stigmasta-7-en-ol (5), 11-hydroxy-10,11-dihydro-euparin (6), 5-hydroxybenzofuran-2(3H) -one- (7), except for beta-sitosterol (8) and daucostrol (9).. Compounds 3 - 7 are isolated for the first time from this genus, the other compounds are obtained from this plant for the first time.

Topics: Asteraceae; Ergosterol; Oleanolic Acid; Pentacyclic Triterpenes; Plants, Medicinal; Rhizome; Sitosterols; Solvents; Spectrometry, Mass, Electrospray Ionization

An activity-guided fractionation method was used to isolate anticancer components from Nuruk (Rhizopus oryzae KSD-815:KSD-815). Dried powder of KSD-815 was extracted with 80% methanol and partitioned successively using nhexane, ethyl acetate, n-butanol, and water. The n-hexane and n-butanol fractions showed a strong antimigratory effect on human cancer cells. Both of these fractions were subjected to separation and purification procedures using silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatographies to afford four purified compounds. These were identified as ergosterol peroxide (1), stigmast- 5-en-3beta,7beta-diol (2), ergosta-7,22-dien-3beta,5alpha,6beta,9alpha-tetraol (3), and daucosterol (4), respectively, by spectroscopic methods such as nuclear magnetic resonance spectrometry, mass spectrometry, and infrared spectroscopy, and comparison with those in the literature. Compounds 1-4 were isolated from KSD-815 for the first time. Compounds 1 and 4 inhibited the migration of MDA-MB-231 cells at concentrations lower than 20 micronM.

Topics: Breast Neoplasms; Cell Line, Tumor; Cell Movement; Edible Grain; Ergosterol; Female; Food Microbiology; Humans; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Structure; Rhizopus; Sitosterols; Spectrophotometry, Infrared; Sterols; Stigmasterol