cysteine has been researched along with iodoacetic acid in 106 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 27 (25.47) | 18.7374 |
| 1990's | 52 (49.06) | 18.2507 |
| 2000's | 14 (13.21) | 29.6817 |
| 2010's | 12 (11.32) | 24.3611 |
| 2020's | 1 (0.94) | 2.80 |
| Authors | Studies |
|---|---|
| Hruz, PW; Miziorko, HM | 1 |
| Gee, N; Gore, MG; Greasley, PJ; Knowles, MR; McAllister, G; Ragan, CI | 1 |
| Pleckaityte, M; Siksnys, V | 1 |
| Alexiev, U; Greenhalgh, DA; Heyn, MP; Khorana, HG; Otto, H; Subramaniam, S | 1 |
| Davis, JP; Van Etten, RL; Zhang, ZY | 1 |
| Kimura, M; Koizumi, S; Otsuka, F | 1 |
| Ash, DE; Chen, CY; Emig, FA; Schramm, VL | 1 |
| Fujioka, M; Konishi, K | 1 |
| Denton, ME; Scheraga, HA | 1 |
| Balhorn, R; Corzett, M; Mazrimas, J; Watkins, B | 1 |
| Knap, AK; Pratt, RF | 1 |
| Johansson, J; Jörnvall, H; Vallee, BL | 1 |
| Atkinson, T; Gore, MG; Scawen, MD; Spencer, P | 1 |
| Rao, US; Scarborough, GA | 1 |
| Ikehara, M; Kanaya, S; Katsuda, C; Kimura, S | 1 |
| Heinrikson, RL; Kohanski, RA | 1 |
| Beinert, H; Howard, JB; Kennedy, MC; Plank, DW | 1 |
| Makinen, AL; Nowak, T | 1 |
| Trageser, M; Unden, G | 1 |
| Flitsch, SL; Khorana, HG | 1 |
| Bosron, WF; Dwulet, FE; Li, TK; Yin, SJ | 1 |
| Brown, SG; Han, PF; Pearson, KH; Zeidan, HM | 1 |
| Krieger, TJ; Miziorko, HM | 1 |
| Bazaes, S; Heinrikson, RL; Kemp, RG; Latshaw, SP; Poorman, RA; Randolph, A | 1 |
| Bergenhem, N; Carlsson, U; Strid, L | 1 |
| Fujioka, M; Konishi, K; Takata, Y | 1 |
| Decottignies, P; Gadal, P; Jacquot, JP; Le Maréchal, P; Miginiac-Maslow, M; Schmitter, JM | 1 |
| Berni, R; Cannella, C; Finazzi-Agrò, A; Rosato, N | 1 |
| Ascari, E; Bottazzi, B; Grignani, G; Mantovani, A; Pacchiarini, L; Pagliarino, M; Ricetti, MM | 1 |
| Fujioka, M; Konishi, K; Ogawa, H; Takata, Y | 1 |
| Bargetzi, JP; Gacond, JJ; Juillerat, MA; Rovelli, G | 1 |
| Chertov, OIu; Lipkin, VM; Seliuchenko, OA | 1 |
| King, MM | 1 |
| Lu, RC; Pliszka, B | 1 |
| Gibson, DD; Hawrylko, J; McCay, PB | 1 |
| Comerford, MJ; de Jongh, KS; Schofield, PJ | 1 |
| Creighton, TE | 1 |
| Hausinger, RP; Howard, JB | 1 |
| Inglis, AS | 1 |
| Barnard, EA; Barron, NM; Dell, A; Gray, AJ | 1 |
| Milner-White, EJ; Murray, S; Price, NC | 1 |
| Ferri, G; Iadarola, P; Zapponi, MC | 1 |
| Fisher, WK; Koureas, DD; Thompson, EO; Webster, DM | 1 |
| Focia, P; Kanaani, J; Maltby, D; Wang, CC | 1 |
| Denu, JM; Dixon, JE; Wu, L; Zhou, G | 1 |
| Lee, MH; Ohta, T; Walker, GC | 1 |
| Cooper, L; Creighton, TE; Zapun, A | 1 |
| Fonda, ML; Gao, G | 1 |
| Bhatnagar, A; Chakrabarti, B; Liu, SQ; Srivastava, SK; Ueno, N | 1 |
| Coulin, F; Magnenat, E; Payton, MA; Proudfoot, AE; Scully, P; Wells, TN | 1 |
| Grubmeyer, C; Segura, E; Teng, H | 1 |
| Ansari, NH; Bhatnagar, A; Liu, SQ; Srivastava, SK | 1 |
| Dixon, JE; Zhang, ZY | 1 |
| Oleinikov, AV; Perroud, B; Traut, RR; Wang, B | 1 |
| Carlson, GM; Cassell, RG; Lewis, CT; Seyer, JM | 1 |
| Deshusses, J; Geindre, M; L'Hostis, C | 1 |
| Caruso, C; Marino, M; Raia, CA; Rossi, M; Vespa, N | 1 |
| Galli, G; Grandi, G; Grifantini, R; Pratesi, C | 1 |
| Chang, WC; Jones, MD; Lu, HS; Narhi, LO | 1 |
| Fahrenholz, F; Kojro, E; Six, S; Trageser, M; Unden, G | 1 |
| Bonicel, J; Morel, MH | 1 |
| Clarke, AE; Ishimizu, T; Liu, YH; Miyagi, M; Norioka, S; Sakiyama, F | 1 |
| Lawson, SL; Wakarchuk, WW; Withers, SG | 1 |
| Kanaani, J; Maltby, D; Somoza, JR; Wang, CC | 1 |
| Bonn, T; Carlquist, M; Engström, O; Hegy, GB; Shackleton, CH; Sjöholm, P; Witkowska, HE | 1 |
| Chew, A; Isaya, G; Rollins, RA; Sakati, WR | 1 |
| Guzzo, A; Lee, MH; Oda, K; Walker, GC | 1 |
| Dadlez, M; Kim, PS | 1 |
| Board, PG; Gali, RR | 1 |
| Carne, AF; U, S | 1 |
| Burstein, Y; Peretz, M; Weiner, LM | 1 |
| Ambrosio, G; Battaglia, C; Cappelli-Bigazzi, M; Chiariello, M; Pannain, S | 1 |
| Beckman, DL; Goldman, BS; Kranz, RG; Monika, EM | 1 |
| Marrone, L; Viswanatha, T | 1 |
| Coll, M; Mantle, TJ; Vega, MC; Walsh, SB | 1 |
| Jung, H; Quick, M | 1 |
| Lundell, N; Schreitmüller, T | 1 |
| Pegg, AE; Stanley, BA; Xiong, H | 1 |
| Chave, KJ; Galivan, J; Ryan, TJ | 1 |
| Petersen, TE; Rasmussen, JT; Rasmussen, MS | 1 |
| Kaback, HR; Sahin-Tóth, M | 1 |
| Bantjes, A; Dijkstra, PJ; Feijen, J; Oberthür, RC; Schrooyen, PM | 1 |
| Choi, M; Hwang, IS; Kim, HJ; Park, SJ; Roh, T | 1 |
| Boja, ES; Fales, HM | 1 |
| Bubis, J; Möller, C; Ortiz, JO | 1 |
| Ding, PZ | 1 |
| Jha, S; Karnani, N; Lynn, AM; Prasad, R | 1 |
| Ballicora, MA; Preiss, J; Sivak, MN; Yep, A | 1 |
| Hsu, SF; Meng, TC; Tonks, NK | 1 |
| Shivaprasad, S; Wetzel, R | 1 |
| Cormier, KS; Fox, JG; Rogers, AB | 1 |
| Chumsae, C; Gaza-Bulseco, G; Liu, H | 1 |
| Chumsae, C; Liu, H; Xiang, T | 1 |
| Hayakawa, T; Kawase, N; Kishi, J; Nakamura, H | 1 |
| Ash, DE; Rodríguez, SB; Stitt, BL | 1 |
| Shirahata, A; Wada, M | 1 |
| Artemenko, KA; Gorshkov, VA; Kanawati, B; Lebedev, AT; Nifant'ev, IE; Samgina, TY; Schmitt-Kopplin, P; Vorontsov, EA; Zubarev, RA | 1 |
| Kuyama, H; Sato, TA; Shimada, T; Tanaka, K | 1 |
| Anand, RP; B Ningappa, M; Balaram, H; Mehrotra, S; Raman, J | 1 |
| McCooeye, M; Mester, Z; Rao, Y | 1 |
| Kaltashov, IA; Wang, S | 2 |
| Grant, GA | 1 |
| Arai, H; Hatano, K; Koyama, T; Matsuoka, K; Matsushita, T; Nemoto, N | 1 |
| Bulleid, N; Burchmore, R; Kabli, AMF; Prakash, AS | 1 |
| Deb-Choudhury, S; Grosvenor, AJ; Harland, DP; Miller, RE; Plowman, JE; Thomas, A | 1 |
106 other study(ies) available for cysteine and iodoacetic acid
| Article | Year |
|---|---|
Avian 3-hydroxy-3-methylglutaryl-CoA lyase: sensitivity of enzyme activity to thiol/disulfide exchange and identification of proximal reactive cysteines.
Topics: Acyl Coenzyme A; Amino Acid Sequence; Animals; Binding Sites; Birds; Cysteine; Disulfides; Iodoacetates; Iodoacetic Acid; Kinetics; Models, Structural; Molecular Sequence Data; Oxidation-Reduction; Oxo-Acid-Lyases; Recombinant Proteins; Sulfhydryl Compounds; Sulfhydryl Reagents | 1992 |
Bovine inositol monophosphatase. Modification, identification and mutagenesis of reactive cysteine residues.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cattle; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Fluorescence Polarization; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Mutagenesis; Peptide Mapping; Phosphoric Monoester Hydrolases | 1992 |
Role of the reactive cysteine residue in restriction endonuclease Cfr9I.
Topics: Base Sequence; Cysteine; Deoxyribonucleases, Type II Site-Specific; Dithionitrobenzoic Acid; DNA Restriction Enzymes; Ethylmaleimide; Hydrogen-Ion Concentration; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Oligonucleotides | 1992 |
Effect of introducing different carboxylate-containing side chains at position 85 on chromophore formation and proton transport in bacteriorhodopsin.
Topics: Amino Acid Sequence; Aspartic Acid; Bacteriorhodopsins; Cysteine; Glutamates; Glutamic Acid; Halobacterium salinarum; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Kinetics; Mutagenesis, Site-Directed; Protons; Recombinant Proteins | 1992 |
Covalent modification and active site-directed inactivation of a low molecular weight phosphotyrosyl protein phosphatase.
Topics: Animals; Arginine; Arsenicals; Binding Sites; Binding, Competitive; Cattle; Cyclohexanones; Cysteine; Epoxy Compounds; Glutathione; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Weight; Phenylglyoxal; Protein Tyrosine Phosphatases | 1992 |
Detection of carboxymethylmetallothionein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Topics: Animals; Autoradiography; Cells, Cultured; Cysteine; Cytosol; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Indicators and Reagents; Iodoacetates; Iodoacetic Acid; Liver; Metallothionein; Sodium Dodecyl Sulfate; Sulfur Radioisotopes | 1991 |
Inactivation of chicken mitochondrial phosphoenolpyruvate carboxykinase by o-phthalaldehyde.
Topics: Amino Acid Sequence; Animals; Chickens; Chromatography, High Pressure Liquid; Cysteine; Hydrogen-Ion Concentration; Indoles; Iodoacetates; Iodoacetic Acid; Mitochondria; Molecular Sequence Data; o-Phthalaldehyde; Peptide Mapping; Phosphoenolpyruvate Carboxykinase (GTP); Sequence Alignment; Spectrometry, Fluorescence; Substrate Specificity | 1991 |
Reversible inactivation of recombinant rat liver guanidinoacetate methyltransferase by glutathione disulfide.
Topics: Adenosine; Animals; Cysteine; Enzyme Activation; Enzyme Reactivators; Ethylmaleimide; Glutathione; Glutathione Disulfide; Glycine; Guanidinoacetate N-Methyltransferase; Iodoacetates; Iodoacetic Acid; Kinetics; Liver; Mercaptoethanol; Methyltransferases; Oxidation-Reduction; Rats; Recombinant Proteins; S-Adenosylmethionine; Spectrum Analysis; Sulfhydryl Compounds | 1991 |
Spectroscopic, immunochemical, and thermodynamic properties of carboxymethyl(Cys6, Cys127)-hen egg white lysozyme.
Topics: Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Hydrogen-Ion Concentration; Iodine Radioisotopes; Iodoacetates; Iodoacetic Acid; Methylation; Muramidase; Peptide Mapping; Protein Conformation; Thermodynamics; Trypsin | 1991 |
Identification of bull protamine disulfides.
Topics: Amino Acid Sequence; Animals; Cattle; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Iodoacetates; Iodoacetic Acid; Male; Models, Molecular; Molecular Sequence Data; Peptide Fragments; Protamines; Protein Conformation; Spermatozoa; Tritium; Trypsin | 1991 |
Inactivation of the RTEM-1 cysteine beta-lactamase by iodoacetate. The nature of active-site functional groups and comparisons with the native enzyme.
Topics: beta-Lactamase Inhibitors; beta-Lactamases; Binding Sites; Boric Acids; Catalysis; Cysteine; Escherichia coli; Genes, Bacterial; Hydrogen-Ion Concentration; Hydrolysis; Iodoacetates; Iodoacetic Acid; Penicillin G; Plasmids; Structure-Activity Relationship | 1991 |
Closely related isozymes of alcohol dehydrogenase. Carboxymethylation: gamma 1 gamma 1 differs widely from both beta 1 beta 1 and its equine equivalence EE.
Topics: Alcohol Dehydrogenase; Animals; Binding Sites; Buffers; Carboxylic Acids; Cysteine; Horses; Iodoacetates; Iodoacetic Acid; Isoenzymes; Methylation; Zinc | 1991 |
The identification of a structurally important cysteine residue in the glycerol dehydrogenase from Bacillus stearothermophilus.
Topics: Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Cobalt; Cysteine; Diethyl Pyrocarbonate; Dithionitrobenzoic Acid; Geobacillus stearothermophilus; Histidine; Iodoacetates; Iodoacetic Acid; Kinetics; Macromolecular Substances; Methyl Methanesulfonate; Molecular Weight; Peptide Fragments; Sugar Alcohol Dehydrogenases; Tritium; Trypsin; Zinc | 1991 |
Chemical state of the cysteine residues in the Neurospora crassa plasma membrane H(+)-ATPase.
Topics: Cell Membrane; Chromatography, Gel; Chromatography, High Pressure Liquid; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Iodoacetates; Iodoacetic Acid; Kinetics; Neurospora; Neurospora crassa; Peptide Fragments; Proton-Translocating ATPases; Sulfhydryl Compounds; Trypsin | 1990 |
Role of cysteine residues in ribonuclease H from Escherichia coli. Site-directed mutagenesis and chemical modification.
Topics: Amino Acid Sequence; Chemical Phenomena; Chemistry; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Endoribonucleases; Escherichia coli; Ethylmaleimide; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Mapping; Ribonuclease H; Serine Endopeptidases; Structure-Activity Relationship | 1990 |
Primary structure of avian hepatic rhodanese.
Topics: Amino Acid Sequence; Amino Acids; Animals; Cattle; Chickens; Cyanogen Bromide; Cysteine; Iodoacetates; Iodoacetic Acid; Liver; Molecular Sequence Data; Peptide Fragments; Sequence Homology, Nucleic Acid; Thiosulfate Sulfurtransferase; Trypsin | 1990 |
Cysteine labeling studies of beef heart aconitase containing a 4Fe, a cubane 3Fe, or a linear 3Fe cluster.
Topics: Aconitate Hydratase; Alkylation; Amino Acid Sequence; Animals; Cattle; Cysteine; Disulfides; Edetic Acid; Ferricyanides; Iodoacetates; Iodoacetic Acid; Iron; Kinetics; Molecular Sequence Data; Myocardium; Oxidation-Reduction; Sequence Homology, Nucleic Acid; Swine | 1989 |
A reactive cysteine in avian liver phosphoenolpyruvate carboxykinase.
Topics: Animals; Binding Sites; Chickens; Cysteine; Electron Spin Resonance Spectroscopy; Iodoacetates; Iodoacetic Acid; Kinetics; Liver; Manganese; Phosphoenolpyruvate Carboxykinase (GTP); Protein Binding | 1989 |
Role of cysteine residues and of metal ions in the regulatory functioning of FNR, the transcriptional regulator of anaerobic respiration in Escherichia coli.
Topics: Alkylating Agents; Amino Acid Sequence; Anaerobiosis; Bacterial Proteins; Chelating Agents; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Immunoblotting; Iodoacetates; Iodoacetic Acid; Iron-Sulfur Proteins; Metals; Molecular Sequence Data; Oxidation-Reduction; Phenanthrolines; Transcription Factors | 1989 |
Structural studies on transmembrane proteins. 1. Model study using bacteriorhodopsin mutants containing single cysteine residues.
Topics: Bacterial Proteins; Base Sequence; Chromatography, High Pressure Liquid; Cysteine; DNA; Escherichia coli; Iodoacetates; Iodoacetic Acid; Membrane Proteins; Models, Chemical; Molecular Sequence Data; Mutation; Plasmids; Protein Conformation; Sulfhydryl Compounds | 1989 |
Selective carboxymethylation of cysteine-174 of the beta 2 beta 2 and beta 1 beta 1 human liver alcohol dehydrogenase isoenzymes by iodoacetate.
Topics: Alcohol Dehydrogenase; Alcohol Oxidoreductases; Amino Acid Sequence; Cysteine; Humans; Iodoacetates; Iodoacetic Acid; Isoenzymes; Kinetics; Liver; Protein Binding | 1986 |
Microenvironment around the essential cysteine residues in chicken liver fructose-1,6-bisphosphatase as analyzed by ESR spin labelling.
Topics: Animals; Chickens; Cysteine; Electron Spin Resonance Spectroscopy; Ethylmaleimide; Fructose-Bisphosphatase; Iodoacetates; Iodoacetic Acid; Liver; Protein Conformation; Spin Labels; Sulfhydryl Compounds | 1986 |
Spinach leaf ribulose-5-phosphate kinase: examination of sulfhydryls by chemical modification and spin-labeling.
Topics: Amino Acids; Chromatography; Cysteine; Electron Spin Resonance Spectroscopy; Iodoacetates; Iodoacetic Acid; Peptide Mapping; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Plants; Spin Labels; Sulfhydryl Compounds | 1987 |
Identification of highly reactive cysteinyl and methionyl residues of rabbit muscle phosphofructokinase.
Topics: Allosteric Site; Amino Acid Sequence; Animals; Binding Sites; Chemical Phenomena; Chemistry; Cyclic AMP; Cysteine; Escherichia coli; Fructosephosphates; Geobacillus stearothermophilus; Iodoacetates; Iodoacetic Acid; Methionine; Muscles; Peptide Fragments; Phosphofructokinase-1; Rabbits; Structure-Activity Relationship; Trypsin | 1987 |
The existence of glutathione and cysteine disulfide-linked to erythrocyte carbonic anhydrase from tiger shark.
Topics: Amino Acids; Animals; Carbon Radioisotopes; Carbonic Anhydrases; Cysteine; Erythrocytes; Glutathione; Glutathione Disulfide; Iodoacetates; Iodoacetic Acid; Molecular Weight; Sharks | 1986 |
Recombinant rat liver guanidinoacetate methyltransferase: reactivity and function of sulfhydryl groups.
Topics: Animals; Binding Sites; Cysteine; Disulfides; Dithionitrobenzoic Acid; Guanidinoacetate N-Methyltransferase; In Vitro Techniques; Iodoacetates; Iodoacetic Acid; Kinetics; Liver; Methyltransferases; Rats | 1988 |
Primary structure of the light-dependent regulatory site of corn NADP-malate dehydrogenase.
Topics: Amino Acid Sequence; Carbocysteine; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Iodoacetates; Iodoacetic Acid; Light; Malate Dehydrogenase; Molecular Sequence Data; Oxidation-Reduction; Peptide Fragments; Plants; Thioredoxins; Trypsin; Zea mays | 1988 |
Active site modifications quench intrinsic fluorescence of rhodanese by different mechanisms.
Topics: Animals; Binding Sites; Cattle; Cysteine; Hydrogen Peroxide; Iodoacetates; Iodoacetic Acid; Liver; Selenium; Spectrometry, Fluorescence; Sulfur; Sulfurtransferases; Thiosulfate Sulfurtransferase | 1986 |
Role of tumor proteinases in tumor cell-induced platelet aggregation.
Topics: Adult; Animals; Cell Communication; Cell Line; Cell Membrane; Cysteine; Endopeptidases; Fibrinopeptide A; Humans; Iodoacetates; Iodoacetic Acid; Mice; Neoplasms, Experimental; Platelet Aggregation; Protease Inhibitors | 1986 |
Function and reactivity of sulfhydryl groups of rat liver glycine methyltransferase.
Topics: Amino Acids; Animals; Carbon Radioisotopes; Cysteine; Dithionitrobenzoic Acid; Glycine N-Methyltransferase; Iodoacetates; Iodoacetic Acid; Kinetics; Liver; Methyltransferases; Nitrobenzoates; Peptide Fragments; Rats; Trypsin | 1987 |
Analysis of folding intermediates from the main toxin of Naja naja samarensis containing two blocked cysteines.
Topics: Amino Acid Sequence; Animals; Carbon Radioisotopes; Cysteine; Disulfides; Elapid Venoms; Iodoacetates; Iodoacetic Acid; Kinetics; Peptide Fragments; Protein Binding; Protein Conformation; Trypsin | 1986 |
[Topography of cysteine residues in DNA-dependent RNA-polymerase].
Topics: Amino Acid Sequence; Autoradiography; Chemical Phenomena; Chemistry; Cysteine; DNA-Directed RNA Polymerases; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Hydrolysis; Iodoacetates; Iodoacetic Acid | 1985 |
Modification of the calmodulin-stimulated phosphatase, calcineurin, by sulfhydryl reagents.
Topics: Animals; Binding Sites; Calcium; Calmodulin-Binding Proteins; Cattle; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydroxymercuribenzoates; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Kinetics; Macromolecular Substances; Manganese; Nickel; Sulfhydryl Reagents | 1986 |
Reactivities of thiols in myosin rod: effect of magnesium and ionic strength.
Topics: Animals; Chromatography, High Pressure Liquid; Cysteine; Iodoacetates; Iodoacetic Acid; Macromolecular Substances; Myosins; Osmolar Concentration; Peptide Fragments; Protein Conformation; Protein Denaturation; Rabbits; Sulfhydryl Compounds; Trypsin | 1985 |
GSH-dependent inhibition of lipid peroxidation: properties of a potent cytosolic system which protects cell membranes.
Topics: Animals; Cell Membrane; Cysteine; Cytosol; Dithiothreitol; Glutathione; Iodoacetates; Iodoacetic Acid; Kinetics; Lipid Peroxides; Liver; Male; Mercaptoethanol; Oxygen Consumption; Rats; Rats, Inbred Strains | 1985 |
Comparative inhibition profiles of human brain and mouse liver L-hexonate dehydrogenase.
Topics: Animals; Brain; Carbohydrate Dehydrogenases; Chloromercuribenzoates; Copper; Copper Sulfate; Cysteine; Dose-Response Relationship, Drug; Humans; Iodoacetates; Iodoacetic Acid; Liver; Mercuric Chloride; Mercury; Mice; NADP; p-Chloromercuribenzoic Acid | 1983 |
Disulfide bond formation in proteins.
Topics: Chemical Phenomena; Chemistry; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Paper; Electrophoresis, Polyacrylamide Gel; Glutathione; Indicators and Reagents; Iodoacetates; Iodoacetic Acid; Kinetics; Oxidation-Reduction; Protein Conformation; Protein Processing, Post-Translational; Proteins | 1984 |
Thiol reactivity of the nitrogenase Fe-protein from Azotobacter vinelandii.
Topics: Azotobacter; Carbon Radioisotopes; Cysteine; Iodoacetates; Iodoacetic Acid; Nitrogenase; Oxidoreductases; Protein Binding; Species Specificity | 1983 |
Single hydrolysis method for all amino acids, including cysteine and tryptophan.
Topics: Alkylation; Amino Acids; Animals; Cattle; Chymotrypsinogen; Cysteine; Hydrolysis; Indicators and Reagents; Iodoacetates; Iodoacetic Acid; Methods; Pancreas; Proteins; Pyridines; Tryptophan | 1983 |
Catalytic groups in relation to the structure of hexokinase.
Topics: Amino Acid Sequence; Binding Sites; Carbodiimides; Carbon Radioisotopes; Cysteine; Hexokinase; Iodoacetates; Iodoacetic Acid; Kinetics; Oxidation-Reduction; Protein Conformation; Saccharomyces cerevisiae | 1981 |
The effect of limited proteolysis on rabbit muscle creatine kinase.
Topics: Adenosine Diphosphate; Animals; Binding Sites; Chromatography, Gel; Creatine Kinase; Cysteine; Endopeptidase K; Endopeptidases; Iodoacetates; Iodoacetic Acid; Molecular Weight; Muscles; Protein Binding; Rabbits | 1981 |
Chloroplast glyceraldehyde-3-phosphate dehydrogenase (NADP+). Reactivity of essential cysteine residues in holo- and apoenzyme.
Topics: Apoenzymes; Apoproteins; Chloroplasts; Cysteine; Glyceraldehyde-3-Phosphate Dehydrogenases; Iodoacetates; Iodoacetic Acid; Kinetics; NAD; NADP; Peptide Fragments; Plants; Trypsin | 1981 |
Amino acid sequences containing cysteine or cystine residues in ovalbumin from eggs of the quail Coturnix coturnix japonica.
Topics: Amino Acid Sequence; Animals; Coturnix; Cysteine; Cystine; Female; Iodoacetates; Iodoacetic Acid; Ovalbumin; Quail | 1981 |
Identification of the active sites of human and schistosomal hypoxanthine-guanine phosphoribosyltransferases by GMP-2',3'-dialdehyde affinity labeling.
Topics: Affinity Labels; Amino Acid Sequence; Animals; Arginine; Binding Sites; Chromatography, High Pressure Liquid; Cysteine; Guanosine Monophosphate; Humans; Hypoxanthine Phosphoribosyltransferase; Iodoacetates; Iodoacetic Acid; Models, Molecular; Molecular Sequence Data; Peptide Fragments; Phosphoribosyl Pyrophosphate; Schistosoma mansoni; Sequence Analysis; Trypsin | 1995 |
The catalytic role of Cys124 in the dual specificity phosphatase VHR.
Topics: Amino Acid Sequence; Base Sequence; Binding Sites; Brain; Cysteine; DNA Primers; Dual Specificity Phosphatase 3; Electrophoresis, Polyacrylamide Gel; Gene Library; Humans; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Sequence Data; Phosphoprotein Phosphatases; Polymerase Chain Reaction; Protein Phosphatase 1; Protein Tyrosine Phosphatases; Recombinant Proteins; Substrate Specificity; Vaccinia virus | 1994 |
A monocysteine approach for probing the structure and interactions of the UmuD protein.
Topics: Bacterial Proteins; Cysteine; DNA-Directed DNA Polymerase; Escherichia coli; Escherichia coli Proteins; Glutaral; Iodoacetates; Iodoacetic Acid; Macromolecular Substances; Maleimides; Mutagenesis; Mutagenesis, Site-Directed; Phenanthrolines; Rec A Recombinases; Recombinant Proteins; Structure-Activity Relationship; Ultraviolet Rays | 1994 |
Replacement of the active-site cysteine residues of DsbA, a protein required for disulfide bond formation in vivo.
Topics: Base Sequence; Binding Sites; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Glutathione; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Isomerases; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Polymerase Chain Reaction; Protein Conformation; Protein Disulfide-Isomerases; Serine; Thermodynamics | 1994 |
Identification of an essential cysteine residue in pyridoxal phosphatase from human erythrocytes.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Erythrocytes; Ethylmaleimide; Humans; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Sequence Data; Peptide Fragments; Phosphoric Monoester Hydrolases; Protein Binding; Sulfhydryl Reagents | 1994 |
Human placental aldose reductase: role of Cys-298 in substrate and inhibitor binding.
Topics: Aldehyde Reductase; Aldehydes; Circular Dichroism; Cysteine; Deuterium; Female; Humans; Imidazoles; Imidazolidines; Iodoacetates; Iodoacetic Acid; Kinetics; NADP; Naphthalenes; Placenta; Pregnancy; Quercetin; Spectrophotometry, Ultraviolet | 1994 |
Identification of Cys-150 in the active site of phosphomannose isomerase from Candida albicans.
Topics: Amino Acid Sequence; Binding Sites; Candida albicans; Catalysis; Cysteine; Fungal Proteins; Iodoacetates; Iodoacetic Acid; Kinetics; Mannose-6-Phosphate Isomerase; Metalloproteins; Molecular Sequence Data; Peptide Fragments; Zinc | 1993 |
Conserved cysteine residues of histidinol dehydrogenase are not involved in catalysis. Novel chemistry required for enzymatic aldehyde oxidation.
Topics: Alcohol Oxidoreductases; Aldehydes; Amino Acid Sequence; Base Sequence; Binding Sites; Catalysis; Chromatography, High Pressure Liquid; Cloning, Molecular; Conserved Sequence; Cysteine; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Oxidation-Reduction; Peptide Fragments; Recombinant Proteins; Salmonella typhimurium | 1993 |
Identification of the reactive cysteine residue in human placenta aldose reductase.
Topics: Adenosine Diphosphate Ribose; Aldehyde Reductase; Amino Acid Sequence; Binding Sites; Carbon Radioisotopes; Cysteine; Humans; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Sequence Data; NADP; Placenta; Trypsin | 1993 |
Active site labeling of the Yersinia protein tyrosine phosphatase: the determination of the pKa of the active site cysteine and the function of the conserved histidine 402.
Topics: Amino Acid Sequence; Base Sequence; Binding Sites; Cysteine; DNA, Single-Stranded; Histidine; Hydrogen-Ion Concentration; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Tyrosine Phosphatases; Yersinia | 1993 |
Structural and functional domains of Escherichia coli ribosomal protein L7/L12. The hinge region is required for activity.
Topics: Amino Acid Sequence; Bacterial Proteins; Chromatography, Gel; Cloning, Molecular; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genes, Bacterial; Genetic Variation; Iodoacetates; Iodoacetic Acid; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Proteins; Restriction Mapping; Ribosomal Proteins; Ribosomes; Sequence Deletion | 1993 |
Identification of vicinal thiols of phosphoenolpyruvate carboxykinase (GTP).
Topics: Alkylation; Amino Acid Sequence; Animals; Azides; Chromatography, High Pressure Liquid; Cysteine; Cystine; Cytosol; Disulfides; Dithionitrobenzoic Acid; Guanosine Triphosphate; Iodoacetates; Iodoacetic Acid; Liver; Molecular Sequence Data; Peptide Fragments; Phosphoenolpyruvate Carboxykinase (GTP); Rats; Sulfhydryl Compounds; Trypsin | 1993 |
Active transport of L-proline in the protozoan parasite Trypanosoma brucei brucei.
Topics: Adenosine Triphosphate; Alanine; Animals; Azetidinecarboxylic Acid; Binding, Competitive; Biological Transport, Active; Cell Membrane Permeability; Cysteine; Digitonin; Hydrogen-Ion Concentration; Hydroxyproline; Iodoacetates; Iodoacetic Acid; Kinetics; Potassium Cyanide; Proline; Temperature; Thermodynamics; Trypanosoma brucei brucei | 1993 |
Activation of Sulfolobus solfataricus alcohol dehydrogenase by modification of cysteine residue 38 with iodoacetic acid.
Topics: Alcohol Dehydrogenase; Alkylating Agents; Buffers; Cysteine; Enzyme Activation; Enzyme Stability; Hydrogen-Ion Concentration; Iodoacetates; Iodoacetic Acid; Kinetics; NAD; Protein Conformation; Sulfolobus; Temperature | 1996 |
Topological mapping of the cysteine residues of N-carbamyl-D-amino-acid amidohydrolase and their role in enzymatic activity.
Topics: Alanine; Amidohydrolases; Amino Acid Sequence; Base Sequence; Chromatography, High Pressure Liquid; Cysteine; DNA Primers; Genes, Bacterial; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Plasmids; Point Mutation; Recombinant Proteins; Restriction Mapping; Rhizobium; Serine | 1996 |
Refolding and oxidation of recombinant human stem cell factor produced in Escherichia coli.
Topics: Amino Acid Sequence; Chromatography, Gel; Chromatography, High Pressure Liquid; Circular Dichroism; Cloning, Molecular; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Humans; Iodoacetates; Iodoacetic Acid; Kinetics; Light; Mutagenesis, Site-Directed; Oxidation-Reduction; Peptide Fragments; Peptide Mapping; Point Mutation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Scattering, Radiation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Stem Cell Factor | 1996 |
Reactivity of the N-terminal cysteine residues in active and inactive forms of FNR, and O2-responsive, Fe containing transcriptional regulator of Escherichia coli.
Topics: Aerobiosis; Alkylation; Amino Acid Sequence; Anaerobiosis; Bacterial Proteins; Binding Sites; Chromatography, Gel; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Iodoacetates; Iodoacetic Acid; Iron; Iron-Sulfur Proteins; Molecular Sequence Data; Molecular Weight; Transcription Factors; Transcription, Genetic; Tritium | 1996 |
Determination of the number of cysteine residues in high molecular weight subunits of wheat glutenin.
Topics: Cysteine; Electrophoresis, Polyacrylamide Gel; Evaluation Studies as Topic; Glutens; Indicators and Reagents; Iodoacetates; Iodoacetic Acid; Molecular Weight; Pyridines; Triticum | 1996 |
Identification of histidine 31 and cysteine 95 in the active site of self-incompatibility associated S6-RNase in Nicotiana alata.
Topics: Amino Acid Sequence; Binding Sites; Chromosome Mapping; Cysteine; Enzyme Inhibitors; Genes, Plant; Histidine; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Nicotiana; Plants, Toxic; Polymorphism, Genetic; Ribonucleases | 1995 |
Effects of both shortening and lengthening the active site nucleophile of Bacillus circulans xylanase on catalytic activity.
Topics: Amino Acid Sequence; Aspartic Acid; Bacillus; Base Sequence; Binding Sites; Carbohydrate Conformation; Carbohydrate Sequence; Catalysis; Cysteine; Glutamic Acid; Glycoside Hydrolases; Glycosylation; Iodoacetates; Iodoacetic Acid; Kinetics; Mass Spectrometry; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Point Mutation; Xylan Endo-1,3-beta-Xylosidase; Xylosidases | 1996 |
Probing the active site of Tritrichomonas foetus hypoxanthine-guanine-xanthine phosphoribosyltransferase using covalent modification of cysteine residues.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Iodoacetates; Iodoacetic Acid; Models, Molecular; Molecular Sequence Data; Pentosyltransferases; Sequence Analysis; Sulfhydryl Reagents; Tritrichomonas foetus | 1996 |
Carboxymethylation of the human estrogen receptor ligand-binding domain-estradiol complex: HPLC/ESMS peptide mapping shows that cysteine 447 does not react with iodoacetic acid.
Topics: Alkylation; Amino Acid Sequence; Binding Sites; Chromatography, High Pressure Liquid; Cysteine; Estradiol; Humans; Iodoacetates; Iodoacetic Acid; Mass Spectrometry; Methylation; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Protein Conformation; Protein Denaturation; Receptors, Estrogen | 1996 |
Mutations in a putative zinc-binding domain inactivate the mitochondrial intermediate peptidase.
Topics: Amino Acid Sequence; Binding Sites; Cloning, Molecular; Codon; Conserved Sequence; Cysteine; DNA Mutational Analysis; Enzyme Stability; Escherichia coli; Ethylmaleimide; Iodoacetates; Iodoacetic Acid; Metalloendopeptidases; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxygen Consumption; Protein Precursors; Recombinant Fusion Proteins; Restriction Mapping; Saccharomyces cerevisiae; Zinc Fingers | 1996 |
Analysis of the region between amino acids 30 and 42 of intact UmuD by a monocysteine approach.
Topics: Amino Acid Sequence; Bacterial Proteins; Cross-Linking Reagents; Cysteine; Dithiothreitol; DNA-Directed DNA Polymerase; Escherichia coli; Escherichia coli Proteins; Iodine; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Mutagenesis; Phenanthrolines; Rec A Recombinases; Sequence Homology, Amino Acid; Structure-Activity Relationship; Ultraviolet Rays | 1996 |
Rapid formation of the native 14-38 disulfide bond in the early stages of BPTI folding.
Topics: Amino Acid Sequence; Animals; Aprotinin; Cattle; Cloning, Molecular; Cysteine; Disulfides; Escherichia coli; Glutathione; Glutathione Disulfide; Indicators and Reagents; Iodoacetates; Iodoacetic Acid; Kinetics; Models, Chemical; Models, Structural; Molecular Sequence Data; Peptide Fragments; Protein Folding; Recombinant Proteins; Urea | 1996 |
Identification of an essential cysteine residue in human glutathione synthase.
Topics: Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Glutathione Synthase; Humans; Iodoacetates; Iodoacetic Acid; Mutagenesis, Site-Directed; Phencyclidine | 1997 |
Chemical modification of proteins for sequence analysis.
Topics: Acrylamide; Acrylamides; Alkylation; Amino Acids; Cysteine; Formates; Iodoacetates; Iodoacetic Acid; Lysine; Oxidation-Reduction; Phenylthiohydantoin; Proteins; Pyridines; Sequence Analysis; Succinic Anhydrides | 1997 |
Cysteine reactivity in Thermoanaerobacter brockii alcohol dehydrogenase.
Topics: Alcohol Dehydrogenase; Bacteria, Anaerobic; Cysteine; Disulfides; Dithiothreitol; Electron Spin Resonance Spectroscopy; Gram-Positive Asporogenous Rods, Irregular; Guanidine; Guanidines; Iodoacetates; Iodoacetic Acid; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Proteins; Spin Labels; Thermodynamics | 1997 |
Role of oxidative metabolism on endothelium-dependent vascular relaxation of isolated vessels.
Topics: Acetylcholine; Adenosine Triphosphate; Amobarbital; Animals; Aorta; Calcimycin; Cysteine; Deoxyglucose; Dinoprost; Electron Transport; Endothelium, Vascular; Glycolysis; Iodoacetates; Iodoacetic Acid; Ionophores; Male; Mitochondria; Nitric Oxide; Nitroglycerin; Organ Culture Techniques; Oxidation-Reduction; Rabbits; Rotenone; S-Nitrosothiols; Uncoupling Agents; Vasodilation; Vasodilator Agents | 1997 |
A thioreduction pathway tethered to the membrane for periplasmic cytochromes c biogenesis; in vitro and in vivo studies.
Topics: Amino Acid Sequence; Bacterial Proteins; Cell Membrane; Cysteine; Cytochrome c Group; Dithiothreitol; Escherichia coli; Genes, Bacterial; Glutathione; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Lyases; Membrane Proteins; Molecular Sequence Data; Oxidation-Reduction; Protein Sorting Signals; Recombinant Fusion Proteins; Rhodobacter capsulatus; Sulfhydryl Reagents | 1997 |
Effect of selective cysteine --> alanine replacements on the catalytic functions of lysine: N6-hydroxylase.
Topics: 2,6-Dichloroindophenol; Alanine; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cyanogen Bromide; Cysteine; Dithionitrobenzoic Acid; Flavoproteins; Iodoacetates; Iodoacetic Acid; Lysine; Mass Spectrometry; Mixed Function Oxygenases; Molecular Sequence Data; Mutagenesis, Site-Directed; NADP; Peptide Fragments; Protein Conformation; Recombinant Proteins; Sequence Analysis; Trypsin | 1997 |
The three-dimensional structure of Cys-47-modified mouse liver glutathione S-transferase P1-1. Carboxymethylation dramatically decreases the affinity for glutathione and is associated with a loss of electron density in the alphaB-310B region.
Topics: Animals; Binding Sites; Crystallography, X-Ray; Cysteine; Glutathione; Glutathione S-Transferase pi; Glutathione Transferase; Iodoacetates; Iodoacetic Acid; Isoenzymes; Liver; Mice; Models, Molecular; Molecular Sequence Data; Protein Conformation | 1998 |
A conserved aspartate residue, Asp187, is important for Na+-dependent proline binding and transport by the Na+/proline transporter of Escherichia coli.
Topics: Amino Acid Sequence; Amino Acid Substitution; Amino Acid Transport Systems, Neutral; Aspartic Acid; Biological Transport, Active; Blotting, Western; Conserved Sequence; Cysteine; Iodoacetamide; Iodoacetic Acid; Kinetics; Ligands; Membrane Transport Proteins; Molecular Sequence Data; Proline; Protein Binding; Sequence Homology, Amino Acid; Sodium | 1998 |
Sample preparation for peptide mapping--A pharmaceutical quality-control perspective.
Topics: Alkylation; Aziridines; Chemistry, Pharmaceutical; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Indicators and Reagents; Interferons; Iodoacetamide; Iodoacetic Acid; Oxidation-Reduction; Peptide Mapping; Peptides; Phosphines; Protein Denaturation; Pyridines; Quality Control; Solubility; Solvents; Trypsin | 1999 |
Role of cysteine-82 in the catalytic mechanism of human S-adenosylmethionine decarboxylase.
Topics: Adenosylmethionine Decarboxylase; Catalysis; Cysteine; Enzyme Activation; Enzyme Inhibitors; Histidine; Humans; Hydrogen-Ion Concentration; Iodoacetic Acid; Kinetics; Mutagenesis, Site-Directed; Recombinant Fusion Proteins; S-Adenosylmethionine; Substrate Specificity | 1999 |
Site-directed mutagenesis establishes cysteine-110 as essential for enzyme activity in human gamma-glutamyl hydrolase.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Binding Sites; Conserved Sequence; Cysteine; DNA Primers; gamma-Glutamyl Hydrolase; Humans; Iodoacetic Acid; Kinetics; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Rats; Recombinant Proteins; Sequence Alignment; Sequence Homology, Amino Acid | 1999 |
Cysteines involved in the interconversion between dehydrogenase and oxidase forms of bovine xanthine oxidoreductase.
Topics: Amino Acid Sequence; Animals; Cattle; Chromatography, High Pressure Liquid; Cysteine; Dithioerythritol; Iodoacetic Acid; Molecular Sequence Data; Oxidation-Reduction; Structure-Activity Relationship; Sulfhydryl Reagents; Xanthine Dehydrogenase; Xanthine Oxidase | 2000 |
Functional conservation in the putative substrate binding site of the sucrose permease from Escherichia coli.
Topics: Alkylation; Arginine; Aspartic Acid; Binding Sites; Biological Transport, Active; Chromatography, Affinity; Conserved Sequence; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Iodoacetic Acid; Membrane Transport Proteins; Mutagenesis, Site-Directed; Serine; Substrate Specificity; Sucrose | 2000 |
Partially carboxymethylated feather keratins. 2. Thermal and mechanical properties of films.
Topics: Animals; Biomechanical Phenomena; Calorimetry, Differential Scanning; Chickens; Cysteine; Feathers; Glycerol; Iodoacetic Acid; Keratins; Molecular Structure; Protein Structure, Secondary; Thermodynamics; Water | 2001 |
Investigation of sulfhydryl groups in cabbage phospholipase D by combination of derivatization methods and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Topics: Brassica; Cysteine; DNA, Complementary; Ethylmaleimide; Iodoacetic Acid; p-Chloromercuribenzoic Acid; Phospholipase D; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin | 2001 |
Overalkylation of a protein digest with iodoacetamide.
Topics: Actins; Alkylation; Animals; Cattle; Chromatography, Liquid; Cysteine; Hydrolysis; Iodoacetamide; Iodoacetic Acid; Mass Spectrometry; Oxidation-Reduction; Peptides; Proteins; Sulfhydryl Reagents | 2001 |
Chemical modification of transducin with iodoacetic acid: transducin-alpha carboxymethylated at Cys(347) allows transducin binding to Light-activated rhodopsin but prevents its release in the presence of GTP.
Topics: Animals; Cattle; Chromatography, High Pressure Liquid; Cysteine; Guanine; Guanosine Diphosphate; Guanosine Triphosphate; Iodoacetic Acid; Light; Magnetic Resonance Spectroscopy; Models, Molecular; Nucleotides; Peptides; Protein Binding; Protein Conformation; Retina; Rhodopsin; Thiocyanates; Time Factors; Transducin; Trypsin | 2001 |
An investigation of cysteine mutants on the cytoplasmic loop X/XI in the melibiose transporter of Escherichia coli by using thiol reagents: implication of structural conservation of charged residues.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acids; Animals; Biological Transport; Cells, Cultured; Cysteine; Escherichia coli Proteins; Iodoacetic Acid; Melibiose; Mesylates; Mutation; Protein Structure, Secondary; Sulfhydryl Reagents; Symporters | 2003 |
Covalent modification of cysteine 193 impairs ATPase function of nucleotide-binding domain of a Candida drug efflux pump.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Amino Acid Sequence; Candida; Catalysis; Cations; Cysteine; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Ethylmaleimide; Hydrolysis; Iodoacetic Acid; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Protein Structure, Tertiary; Sequence Homology, Amino Acid | 2003 |
Identification and characterization of a critical region in the glycogen synthase from Escherichia coli.
Topics: Adenosine Diphosphate Glucose; Adenosine Monophosphate; Amino Acid Sequence; Binding Sites; Computer Simulation; Conserved Sequence; Crystallization; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Escherichia coli; Gene Expression; Glucosephosphates; Glycogen Synthase; Iodoacetic Acid; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Structure-Activity Relationship; Substrate Specificity | 2004 |
Development of a modified in-gel assay to identify protein tyrosine phosphatases that are oxidized and inactivated in vivo.
Topics: Alkylation; Cysteine; Electrophoresis, Polyacrylamide Gel; Humans; Iodoacetic Acid; Protein Tyrosine Phosphatases | 2005 |
Scanning cysteine mutagenesis analysis of Abeta-(1-40) amyloid fibrils.
Topics: Amino Acid Sequence; Amyloid beta-Peptides; Benzothiazoles; Chromatography, High Pressure Liquid; Cysteine; Cystine; Electron Spin Resonance Spectroscopy; Humans; Hydrocarbons, Iodinated; Iodoacetic Acid; Kinetics; Microscopy, Electron; Models, Molecular; Molecular Sequence Data; Mutagenesis; Mutation; Peptide Fragments; Peptide Mapping; Peptides; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Solvents; Thermodynamics; Thiazoles; Time Factors | 2006 |
Thiol-reactive compounds prevent nonspecific antibody binding in immunohistochemistry.
Topics: Animals; Antibodies; Antibody Specificity; Cysteine; Dithionitrobenzoic Acid; Enzyme-Linked Immunosorbent Assay; Glutathione; Immunohistochemistry; Indicators and Reagents; Iodoacetic Acid; Ligands; Mice; Oxidation-Reduction; Sensitivity and Specificity; Sulfhydryl Compounds; Tissue Fixation | 2006 |
Identification and localization of unpaired cysteine residues in monoclonal antibodies by fluorescence labeling and mass spectrometry.
Topics: Antibodies, Monoclonal; Carbon Radioisotopes; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Fluoresceins; Fluorescence; Humans; Immunoglobulin G; Iodoacetic Acid; Recombinant Proteins; Spectrometry, Fluorescence; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds | 2009 |
Localization and quantitation of free sulfhydryl in recombinant monoclonal antibodies by differential labeling with 12C and 13C iodoacetic acid and LC-MS analysis.
Topics: Amino Acid Sequence; Antibodies, Monoclonal; Carbon Isotopes; Carbon Radioisotopes; Chromatography, High Pressure Liquid; Cysteine; Iodoacetic Acid; Mass Spectrometry; Molecular Sequence Data; Recombinant Proteins; Sulfhydryl Compounds | 2009 |
Collagenolytic activity in sonic extracts of Tannerella forsythia.
Topics: Bacteroides; Calcium Chloride; Cathepsins; Cell Line, Tumor; Chelating Agents; Collagen Type I; Cysteine; Cysteine Proteinase Inhibitors; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Enzyme Inhibitors; Enzyme Precursors; Ethylmaleimide; Gelatinases; Humans; Iodoacetamide; Iodoacetic Acid; Leucine; Leupeptins; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Phenylmethylsulfonyl Fluoride; Protease Inhibitors; Serine Proteinase Inhibitors; Tosyllysine Chloromethyl Ketone; Tosylphenylalanyl Chloromethyl Ketone | 2010 |
Cysteine 351 is an essential nucleophile in catalysis by Porphyromonas gingivalis peptidylarginine deiminase.
Topics: Catalysis; Cysteine; Hydrogen-Ion Concentration; Hydrolases; Iodoacetamide; Iodoacetic Acid; Ligands; Mass Spectrometry; Mutagenesis, Site-Directed; Peptide Mapping; Porphyromonas gingivalis; Protein Binding; Protein-Arginine Deiminases; Recombinant Proteins | 2010 |
Conformational stabilization of rat s-adenosylmethionine decarboxylase by putrescine.
Topics: Adenosylmethionine Decarboxylase; Animals; Cysteine; Iodoacetic Acid; Male; Methylation; Molecular Structure; Prostate; Putrescine; Rats; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Trypsin | 2010 |
Novel cysteine tags for the sequencing of non-tryptic disulfide peptides of anurans: ESI-MS study of fragmentation efficiency.
Topics: Amino Acid Sequence; Amphibian Proteins; Animals; Antimicrobial Cationic Peptides; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Female; Iodoacetic Acid; Male; Maleimides; Molecular Probes; Molecular Sequence Data; Rana ridibunda; Sequence Analysis, Protein; Skin; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry | 2011 |
Development of iodoacetic acid-based cysteine mass tags: detection enhancement for cysteine-containing peptide by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Topics: Cysteine; Iodoacetic Acid; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2012 |
Mutational analysis of cysteine 328 and cysteine 368 at the interface of Plasmodium falciparum adenylosuccinate synthetase.
Topics: Adenylosuccinate Synthase; Amino Acid Substitution; Chromatography, Gel; Copper; Cysteine; Enzyme Stability; Iodoacetic Acid; Kinetics; Manganese; Models, Molecular; Mutagenesis, Site-Directed; Plasmodium falciparum; Protein Denaturation; Protein Folding; Protein Interaction Domains and Motifs; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Subunits; Protozoan Proteins; Tryptophan; Urea | 2012 |
Mapping of sulfur metabolic pathway by LC Orbitrap mass spectrometry.
Topics: Arsenites; Cadmium; Chromatography, High Pressure Liquid; Cysteine; Glutathione; Hydrogen Peroxide; Iodoacetic Acid; Mass Spectrometry; Metabolic Networks and Pathways; Oxidation-Reduction; Saccharomyces cerevisiae; Selenium; Sulfhydryl Compounds | 2012 |
A new strategy of using O18-labeled iodoacetic acid for mass spectrometry-based protein quantitation.
Topics: Amino Acid Sequence; Chromatography, Liquid; Cysteine; Feasibility Studies; Humans; Hydrogen-Ion Concentration; Iodoacetic Acid; Isotope Labeling; Molecular Sequence Data; Oxygen Isotopes; Peptide Fragments; Proteins; Spectrometry, Mass, Electrospray Ionization; Transferrin | 2012 |
Identification of reduction-susceptible disulfide bonds in transferrin by differential alkylation using O(16)/O(18) labeled iodoacetic acid.
Topics: Alkylation; Chromatography, High Pressure Liquid; Cysteine; Cystine; Dithiothreitol; Feasibility Studies; Humans; Indicators and Reagents; Iodoacetic Acid; Models, Molecular; Oxidation-Reduction; Oxygen Isotopes; Protein Conformation; Protein Stability; Reducing Agents; Tandem Mass Spectrometry; Transferrin | 2015 |
Modification of Cysteine.
Topics: Alkylation; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Formates; Indicators and Reagents; Iodoacetamide; Iodoacetic Acid; Oxidation-Reduction; Peptides; Proteins; Pyridines | 2017 |
Iodoacetyl-functionalized pullulan: A supplemental enhancer for single-domain antibody-polyclonal antibody sandwich enzyme-linked immunosorbent assay for detection of survivin.
Topics: Animals; Antibodies; Cysteine; Enzyme-Linked Immunosorbent Assay; Glucans; Humans; Inhibitor of Apoptosis Proteins; Iodoacetic Acid; Single-Domain Antibodies; Survivin | 2017 |
Mix-and-Match Proteomics: Using Advanced Iodoacetyl Tandem Mass Tag Multiplexing To Investigate Cysteine Oxidation Changes with Respect to Protein Expression.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Cysteine; Iodoacetic Acid; Leishmania mexicana; Oxidation-Reduction; Oxidative Stress; Proteomics; Protozoan Proteins; Tandem Mass Spectrometry | 2018 |
A detailed mapping of the readily accessible disulphide bonds in the cortex of wool fibres.
Topics: Acrylamide; Alkylation; Amino Acid Sequence; Animals; Chromatography, Liquid; Cysteine; Disulfides; Humans; Iodoacetamide; Iodoacetic Acid; Keratins, Hair-Specific; Peptide Mapping; Protein Isoforms; Protein Multimerization; Sheep, Domestic; Tandem Mass Spectrometry; Wool; Wool Fiber | 2021 |