cysteine has been researched along with iodoacetamide in 237 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 54 (22.78) | 18.7374 |
| 1990's | 77 (32.49) | 18.2507 |
| 2000's | 63 (26.58) | 29.6817 |
| 2010's | 33 (13.92) | 24.3611 |
| 2020's | 10 (4.22) | 2.80 |
| Authors | Studies |
|---|---|
| Foster, JF; Kohr, WJ; Zurawski, VR | 1 |
| Lübbert, H; Pollow, B; Pollow, K | 1 |
| Thorpe, C; Williams, CH | 1 |
| Linde, A; Persliden, B | 1 |
| Bauer, H; Kresze, GB; Lynen, F; Oesterhelt, D; Steber, L | 1 |
| Barnard, GF; Hohberger, LH; Itoh, R; Shemin, D | 1 |
| Schellenberger, A; Ulbrich, R | 1 |
| Danenberg, PV | 1 |
| Porter, M; Weber, A | 1 |
| Byers, LD; Koshland, DE | 1 |
| Waterman, MR | 1 |
| Barnard, EA; Bhargava, AK; Jones, JG; Otieno, S | 1 |
| Gregory, EM | 1 |
| Klinman, JP | 1 |
| Hankin, SR; Kashket, S | 1 |
| Goto, Y; Okazaki, T; Shukuya, R; Tomizuka, T | 1 |
| Burgum, AA; Matthews, KS; Yang, DS | 1 |
| Barnard, EA; Bhargava, AK; Otieno, S; Serelis, D | 1 |
| Kresze, GB; Lynen, F; Oesterhelt, D; Steber, L | 1 |
| Shaw, WV; Zaidenzaig, Y | 1 |
| Auchet, JC; Tawada, K; Wahl, P | 2 |
| Kelsey, DE; Rounds, TC; York, SS | 1 |
| Goil, MM | 1 |
| Flashner, M; Mante, SD; Tanenbaum, SW | 1 |
| Kochkina, VM; Torchinskii, YM | 1 |
| Azuma, T; Hamaguchi, K; Kishida, F | 1 |
| Banerjee, RV; González, JC; Huang, S; Matthews, RG; Sumner, JS | 1 |
| Alexiev, U; Greenhalgh, DA; Heyn, MP; Khorana, HG; Otto, H; Subramaniam, S | 1 |
| Hausinger, RP; Martin, PR | 1 |
| Careaga, CL; Falke, JJ | 1 |
| Defeo-Jones, D; Heimbrook, DC; Maigetter, RZ; Oliff, A; Patrick, DR; Sardana, MK; Vuocolo, GR; Zhang, K | 1 |
| Brown, PL; Mecham, L; Mecham, RP; Tisdale, C | 1 |
| Davis, JP; Van Etten, RL; Zhang, ZY | 1 |
| Ekkel, Y; Freedman, NJ; Krieger, M; Lux, A; McKinstry, H; Penman, M; Resnick, D; Rohrer, L | 1 |
| Ivey, KJ; Raza, A; Razandi, M; Romano, M; Szabo, S | 1 |
| Caine, JM; McLeish, MJ | 1 |
| Chen, WL; Hsieh, JC; Huang, SC; Lai, YC; Tam, MF | 1 |
| Chang, LH; Tam, MF; Wang, LY | 1 |
| Kawamura-Konishi, Y; Suzuki, H | 1 |
| Blackwell, LF; Buckley, PD; Loomes, KM; Midwinter, GG | 1 |
| Bradbury, EM; Marvin, KW; Yau, P | 1 |
| Harlow, KW; Switzer, RL | 1 |
| Detwiler, TC; Speziale, MV | 1 |
| Bakke, JE; Davison, KL | 1 |
| Low, PS; Thevenin, BJ; Willardson, BM | 1 |
| Brown, SB; Roche, RS; Stevenson, KJ; Turner, RJ | 1 |
| Funabashi, H; Iwasaki, S; Kawaguchi, A; Okuda, S; Omura, S; Tomoda, H | 1 |
| Shewchuk, LM; Verdine, GL; Walsh, CT | 1 |
| Bernhard, WR; Kägi, JH; Vasák, M | 1 |
| Coleman, JE; King, GC; Martin, CT; Pham, TT | 1 |
| Hodges, RS; Ingraham, RH | 1 |
| Ghebrehiwet, B; Janoff, A; Kew, RR | 1 |
| Gan, ZR; Wells, WW | 1 |
| King, MM | 1 |
| Knudsen, J; Mikkelsen, J; Smith, S; Stern, A | 1 |
| Gilbert, HF; Izbicka-Dimitrijević, E | 1 |
| Bragg, PD; Stan-Lotter, H | 1 |
| Schaffer, SW; Smith, GK | 1 |
| Capaldi, R; Malatesta, F | 1 |
| Barrett, CI; Schneider, JM; York, SS | 1 |
| Davidson, BE; Hudson, GS; Wong, V | 1 |
| Severin, SE; Shur, SA; Vulfson, PL | 1 |
| Hsu, RY; Tian, WX; Wang, YS | 1 |
| Mego, JL | 1 |
| Malm, B | 1 |
| Katiyar, SS; Pan, D; Porter, JW | 1 |
| Stoops, JK; Wakil, SJ | 1 |
| Kumar, S; Varagiannis, E | 1 |
| Chiu, SS; Lewis, PN | 1 |
| Furuta, H; Kajita, A; Ohe, M | 1 |
| Crookston, KP; Gonias, SL | 1 |
| Boggs, JM; Deber, CM; Khan, AR; Williams, KA | 1 |
| Huete-Pérez, JA; Wang, CC; Whitby, FG; Wu, JC | 1 |
| Davie, EW; Ezban, M; Fujikawa, K; Hedner, U; McMullen, BA | 1 |
| Fairclough, RH; Richman, DP; Xu, Q | 1 |
| Heilmeyer, LM; Meyer, H; Shoshan-Barmatz, V; Varsanyi, M; Weil, S | 1 |
| Honma, M; Kawai, J; Yamada, M | 1 |
| Chang, JY; Ramseier, U | 1 |
| Horie, K; Ishikawa, R; Kohama, K; Sasaki, Y; Tsunomori, F; Ushiki, H; Yamashita, T | 1 |
| Freeman, JA; Hess, DT; Lin, LH; Norden, JJ | 1 |
| Baldwin, JJ; Claremon, DA; Doshi, KP; Freund, KF; Gaul, SL; Pitzenberger, SM; Remy, DC; Stern, AM | 1 |
| Bustin, M; Friedman, FK; Lehn, DA; Postnikov, YV; Robinson, RC; Shiloach, J | 1 |
| Arsequell, G; Dwek, RA; Guile, GR; Wong, SY | 1 |
| Castelhano, AL; Copp, L; Feng, R; Krantz, A; Li, B; Stahl, M; Yuan, Z | 1 |
| Abriola, DP; Blatter, EE; Mukerjee, N; Pietruszko, R | 1 |
| Arnold, SA; Birckbichler, PJ; Carter, HA; Fraij, BM; Lee, KN; Patterson, MK; Takeuchi, Y | 1 |
| Cooper, L; Creighton, TE; Zapun, A | 1 |
| Chernogolov, A; Kraft, R; Schwartz, D; Usanov, S | 1 |
| Accornero, P; Carrera, G; Curti, B; Vanoni, MA | 1 |
| Creighton, TE; Nelson, JW | 1 |
| Epstein, DL; Ludueña, RF; Roach, MC | 1 |
| Cardemil, E; Encinas, MV; Kemp, RG; Latshaw, SP; Rojas, MC | 1 |
| McGinnis, K; Wilson, DB | 2 |
| Neer, EJ; Sladek, T; Smith, T; Thomas, TC; Yi, F | 1 |
| Del Boccio, G; Desideri, A; Falconi, M; Federici, G; Lo Bello, M; Parker, MW; Pennelli, A; Polticelli, F; Ricci, G | 1 |
| Chen, LC; Nagase, H; Noelken, ME | 1 |
| Lewendon, A; Shaw, WV | 1 |
| Inman, JK; Krutzsch, HC | 1 |
| Bardwell, JC; Creighton, TE; Zapun, A | 1 |
| Kalafatis, M; Mann, KG; Xue, J | 1 |
| Fa, M; Ny, T; Ohlsson, PI; Wilczynska, M | 1 |
| Bayley, H; Walker, B | 1 |
| Krantz, A; Li, B; Xia, L; Yuan, Z | 1 |
| Decottignies, P; Issakidis, E; Lemaire, M; Miginiac-Maslow, M; Ruelland, E | 1 |
| Connolly, DT; Glenn, K; Heuvelman, D | 1 |
| Guyer, CA; Staros, JV; Tong, K | 1 |
| Weigel, PH; Zeng, FY | 1 |
| Heacock, DH; Liu, H; Musier-Forsyth, K; Stehlin, C | 1 |
| Chernogolov, AA; Schwarz, D; Usanov, SA | 1 |
| Beckman, DL; Goldman, BS; Kranz, RG; Monika, EM | 1 |
| Carlsson, U; Hammarström, P; Jonsson, BH; Kalman, B | 1 |
| Child, CJ; Shoolingin-Jordan, PM | 1 |
| Hamiche, A; Richard-Foy, H | 1 |
| Carter, CW; Doublié, S; Xue, H; Xue, Y | 1 |
| Rao, DN; Reddy, YV | 1 |
| Jiang, XY; Lapko, VN; Smith, DL; Song, PS | 1 |
| Jung, H; Quick, M | 1 |
| Gunnarsson, M; Jensen, PE | 1 |
| Chait, BT; Sechi, S | 1 |
| Lundell, N; Schreitmüller, T | 1 |
| Washburn, MP; Wells, WW | 1 |
| Bauer, CM; Cross, TA; Lamb, RA; Pinto, LH | 1 |
| Adrait, A; Forest, E; Gonzalez de Peredo, A; Jacquamet, L; Latour, JM; Michaud-Soret, I; Saint-Pierre, C | 1 |
| Bochar, DA; Rodwell, VW; Stauffacher, CV; Tabernero, L | 1 |
| Huang, Y; Liu, M; Wang, E; Wang, Y; Wu, J | 1 |
| Powlowski, J; Sahlman, L | 1 |
| Blanchard, JS; Born, TL | 1 |
| Rao, AG; Sudharshan, E | 1 |
| Jiang, Sq; Paulus, H; Shingledecker, K | 1 |
| Moulin, A; Puigserver, A; Smialowski-Fléter, S; Villard, C | 1 |
| Lapko, VN; Smith, DL; Smith, JB | 1 |
| Fukuma, K; Kagami, J; Sankawa, U; Suh, DY | 1 |
| Jez, JM; Noel, JP | 1 |
| Amrhein, N; Evans, JN; Krekel, F; Macheroux, P; Samland, AK | 1 |
| Dyllick-Brenzinger, M; Liu, M; Taylor, DE; Turner, RJ; Winstone, TL | 1 |
| Bensussan, A; Bismuth, G; Boumsell, L; Delaire, S; Elhabazi, A | 1 |
| Arduini, RM; Baker, DP; Boriack-Sjodin, A; Carmillo, AN; Day, ES; Galdes, A; Garber, EA; Hronowski, X; Pepinsky, RB; Rayhorn, P; Shapiro, RI; Taylor, FR; Weinreb, PH; Wen, D; Whitty, A; Williams, KP | 1 |
| Boeren, S; de Wit, PJ; van den Burg, HA; van den Hooven, HW; Vervoort, J; Vossen, P | 1 |
| Galvani, M; Hamdan, M; Herbert, B; Righetti, PG | 1 |
| Smith, JW; Yan, B | 1 |
| Boja, ES; Fales, HM | 1 |
| Ansorge, S; Arndt, M; Firla, B; Frank, K; Lendeckel, U; Täger, M; Thiel, U | 1 |
| Britto, PJ; Knipling, L; Wolff, J | 1 |
| La Spada, G; Marino, A; Montaleone, B; Musci, G; Soffli, A; Sorrenti, G | 1 |
| Pitts, KE; Summers, AO | 1 |
| Joshi, AK; Smith, S; Witkowski, A | 1 |
| Liang, S; Liu, Z; Peng, K; Shu, Q | 1 |
| Hayward, LJ; Tiwari, A | 1 |
| Carne, AF | 1 |
| Belli, G; Cabiscol, E; Herrero, E; Ros, J; Tamarit, J | 1 |
| Bryson, WG; Fitzgerald, AC; Flanagan, LM; Joyce, NI; Paton, LN; Plowman, JE | 1 |
| Pretzer, E; Tyagarajan, K; Wiktorowicz, JE | 1 |
| Gellon, JO; Girault, HH; Jensen, H; Lion, N | 1 |
| PIERAGNOLI, E; PRETOLANI, E; TURA, S | 1 |
| Popov, VO; Savel'eva, ND; Tikhonova, TV | 1 |
| Corthals, GL; Hochstrasser, DF; Pasquarello, C; Sanchez, JC | 1 |
| Cohen, RA; Costello, CE; Heibeck, T; McComb, ME; Sethuraman, M | 1 |
| Ambahera, S; Beck, JL; de Jersey, J; Ralph, SF; Sheil, MM; Yong, SR | 1 |
| Chevallet, M; Diemer, H; Leize-Wagner, E; Luche, S; Rabilloud, T; Tastet, C; Van Dorsselaer, A | 1 |
| Dawson, JF; Summerscales, J | 1 |
| Gleason, KJ; Hanna, PE; Vath, GM; Wagner, CR; Wang, H | 1 |
| Maurer, MC; Sabo, TM; Turner, BT; Wilding, D | 1 |
| Av-Gay, Y; Fahey, RC; Newton, G; Rawat, M; Steffek, M; Uppal, M | 1 |
| Finnie, C; Maeda, K; Svensson, B | 1 |
| Guillemette, JG; Lang, S; Palmer, M; Spratt, DE | 1 |
| Adachi, Y; Chen, W; Kamata, T; Shang, WH | 1 |
| Eggler, AL; Liu, G; Mesecar, AD; Pezzuto, JM; van Breemen, RB | 1 |
| Bjellqvist, B; Bjerneld, EJ; Cetinkaya, S; Goscinski, J; Grigorescu, I; Haid, D; Hedberg, JJ; Laurin, Y | 1 |
| Cho, B; Karki, P; Lee, J; Park, IS; Shin, SY | 1 |
| Benz, FW; Cai, J; Feng, W; Kang, YJ; Pierce, WM | 1 |
| Alam, M; Gilham, D; Lehner, R; Vance, DE | 1 |
| Branlant, G; Stines-Chaumeil, C; Talfournier, F | 1 |
| Alexander, C; Borg, E; Dawson, DC; Liu, X; Serrano, J | 1 |
| Costello, AL; Fast, W; Stone, EM; Tierney, DL | 1 |
| Bishop, B; Born, TL; Huddler, DP; Mutumanje, E; Noble, SM; Ziegler, K | 1 |
| Brown, DT; Goshe, MB; Hernandez, R; Soderblom, EJ; West, ML; Whitehurst, CB | 1 |
| Liebler, DC; Liu, Q; Shin, NY; Stamer, SL | 1 |
| Cebi, M; Koert, U | 1 |
| Baer, BR; Rock, DA; Wienkers, LC | 1 |
| Banerjee, T; Deo, SK; Rahimi, Y; Shrestha, S | 1 |
| Brasher, PB; Maurer, MC; Sabo, TM | 1 |
| Adachi, T; Clavreul, N; Cohen, RA; Sethuraman, M; Ying, J | 1 |
| Eggler, AL; Luo, Y; Mesecar, AD; van Breemen, RB | 1 |
| Bresnick, AR; Garrett, SC; Hahn, KM; Hodgson, L; Lawrence, DS; Rybin, A; Toutchkine, A | 1 |
| Day, AE; King, SB; Nelson, KJ; Poole, LB; Zeng, BB | 1 |
| Adachi, T; Cohen, RA; Pimentel, DR; Tong, X; Trucillo, M; Ying, J | 1 |
| Bori, ID; Comins, DL; Hawkridge, AM; Meadows, CW; Muddiman, DC; Williams, DK | 1 |
| He, M; Lou, J; Luo, C; Murray, BW; Ryan, K; Solowiej, J; Thomson, JA | 1 |
| Fang, J; Iwasa, KH; Marriott, G; Sakata, T | 1 |
| Hall, A; Karplus, PA; Nelson, KJ; Parsonage, D; Poole, LB | 1 |
| Bruschi, M; Candiano, G; Della Ciana, L; Fabbroni, S; Ghiggeri, GM; Grilli, S; Gusmano, R; Petretto, A; Santucci, L; Scolari, F; Urbani, A | 1 |
| Brodbelt, JS; Vasicek, L | 1 |
| Ishii, T; Mori, T; Nakayama, T; Wakabayashi, M | 1 |
| Kayastha, AM; Kumar, S | 1 |
| Hayakawa, T; Kawase, N; Kishi, J; Nakamura, H | 1 |
| Ash, DE; Rodríguez, SB; Stitt, BL | 1 |
| Florea, B; Hoogendoorn, S; Overkleeft, H; Willems, L | 1 |
| Choi, HI; Chung, KJ; Jeong, SH; Kim, YU; Kwon, J; Lee, TH; Park, BJ; Park, HR; Park, SH; Ren, L; Yang, HY; Yang, U | 1 |
| Clavaud, C; Dugave, C; Le Gal, J; Thai, R | 1 |
| Chan, HL; Sinclair, J; Timms, JF | 1 |
| Darie, CC; Sokolowska, I; Woods, AG | 1 |
| Laurence, JS; Middaugh, CR; Siahaan, TJ; Trivedi, M; Williams, TD | 1 |
| Baba, H; Ishida, A; Kameshita, I; Shigeri, Y; Sueyoshi, N | 1 |
| Jian, Y; Li, L; Lin, G; Nelson, RS; Telser, J; Yang, L | 1 |
| Niwayama, S; Shaikh, AL; Zabet-Moghaddam, M | 1 |
| Bent, AF; Botting, CH; Fuszard, MA; Houssen, WE; Jaspars, M; Koehnke, J; Morawitz, F; Naismith, JH; Shirran, SL; Smellie, IA; Smith, MC | 1 |
| Barretto, K; Duran, S; Hamilton, CJ; Rajkarnikar, A; Rawat, M; Roberts, AA; Strankman, A; Upton, H; Vargas, D | 1 |
| Bechtold, E; Furdui, CM; King, SB; Poole, LB; Reisz, JA | 1 |
| Li, G; Qiao, X; Wang, R; Yan, H; Zhang, L; Zhang, Y; Zhou, Y | 1 |
| Ma, C; Qian, X; Wang, J; Ying, W; Zhao, X; Zhao, Y; Zhong, R | 1 |
| Belshoff, AC; Ehringer, DS; Fan, TW; Gori, SS; Higashi, RM; Lorkiewicz, P; Nantz, MH | 1 |
| Favrot, L; Lajiness, DH; Ronning, DR | 1 |
| Ash, PA; Grabarczyk, DB; Vincent, KA | 1 |
| Guo, XF; Tu, FQ; Wang, H; Wang, P; Zhang, HS; Zhang, LY | 1 |
| Rogowska-Wrzesinska, A; Wojdyla, K | 1 |
| Gammelgaard, B; Nguyen, TT; Østergaard, J | 1 |
| Gottlieb, CD; Linder, ME; Zhang, S | 1 |
| Baalmann, M; Berg, R; Hanke, S; Kerner, A; Nadler, W; Roesli, C; Trumpp, A; Walch, P | 1 |
| Qian, Y; Weerapana, E | 1 |
| Grant, GA | 1 |
| Hains, PG; Robinson, PJ | 1 |
| Abo, M; Li, C; Weerapana, E | 1 |
| Gottlieb, E; Lilla, S; van der Reest, J; Zanivan, S; Zheng, L | 1 |
| Cardoso, MC; Glanz, M; Hackenberger, CPR; Helma, J; Kasper, MA; Klenk, S; Krause, E; Leonhardt, H; Penkert, M; Sauer, T; Schumacher, D; Stengl, A | 1 |
| Akhtar, M; Arif, M; Gardner, QA; Hafeez, S; Hassan, N | 1 |
| Bechtel, TJ; Kisty, EA; Li, C; Maurais, AJ; Weerapana, E | 1 |
| Barr, JT; Min, X; Rock, BM; Rock, DA; Wang, Z; Wienkers, HJ; Wienkers, LC | 1 |
| Brustad, EM; Cruz, CL; Nicewicz, DA; Reynolds, EW; Schwochert, TD; Watters, JW | 1 |
| Cheng, Y; Julius, D; Lin King, JV; Paulsen, CE; Zhao, J | 1 |
| Bubis, JA; Gorshkov, MV; Ilina, IY; Kuznetsova, KG; Levitsky, LI; Moshkovskii, SA; Pyatnitskiy, MA; Solovyeva, EM; Zgoda, VG | 1 |
| Becher, D; Hayashi, M; Jensen, ON; Maaß, S; Palmgren, M; Pedersen, JT; Ravnsborg, T; Stöhr, C; Welle, M | 1 |
| Backus, KM; Boatner, LM; Cao, J; Desai, HS; Jami-Alahmadi, Y; Palafox, MF; Yan, T; Yen, SL | 1 |
| Deb-Choudhury, S; Grosvenor, AJ; Harland, DP; Miller, RE; Plowman, JE; Thomas, A | 1 |
| Barnett, DA; Joy, AP; Murphy, EL; Ouellette, RJ | 1 |
| Banwell, MG; Chen, J; Chew, EH; Hu, J; Karuppasamy, M; Lai, X; Lan, P; Liu, W; Siok-Cheng Lim, B; Tan, SH; Zhang, Y | 1 |
3 review(s) available for cysteine and iodoacetamide
| Article | Year |
|---|---|
Thymidylate synthetase - a target enzyme in cancer chemotherapy.
Topics: Animals; Binding, Competitive; Cysteine; Deoxyuracil Nucleotides; DNA, Neoplasm; Female; Floxuridine; Humans; In Vitro Techniques; Iodoacetamide; Kinetics; Male; Methyltransferases; Models, Chemical; Neoplasms; Tetrahydrofolates; Thionucleotides; Thymidylate Synthase; Thymine Nucleotides | 1977 |
Thiol oxidation in signaling and response to stress: detection and quantification of physiological and pathophysiological thiol modifications.
Topics: Animals; Biotinylation; Cysteine; Glutathione; Iodoacetamide; Isotope Labeling; Mass Spectrometry; Nitrosation; Oxidation-Reduction; Protein Processing, Post-Translational; Proteomics | 2007 |
Differential alkylation-based redox proteomics--Lessons learnt.
Topics: Alkylation; Animals; Arsenites; Ascorbic Acid; Cysteine; Dithiothreitol; Ethylmaleimide; Eukaryotic Cells; Humans; Iodoacetamide; Models, Biological; Oxidation-Reduction; Phosphines; Proteomics; Staining and Labeling; Sulfhydryl Compounds | 2015 |
234 other study(ies) available for cysteine and iodoacetamide
| Article | Year |
|---|---|
Conformational properties of bovine plasma albumin with a cleaved internal peptide bond.
Topics: Amino Acids; Anilino Naphthalenesulfonates; Animals; Binding Sites; Cattle; Circular Dichroism; Cysteine; Hydrogen-Ion Concentration; Iodoacetamide; Macromolecular Substances; Molecular Weight; Optical Rotation; Peptide Hydrolases; Protein Binding; Protein Conformation; Serum Albumin, Bovine | 1975 |
On the mitochondrial 17beta-hydroxysteroid dehydrogenase from human endometrium and endometrial carcinoma: characterization and intramitochondrial distribution.
Topics: Cations, Divalent; Chloromercuribenzoates; Cysteine; Endometrium; Estradiol Dehydrogenases; Female; Humans; Hydrogen-Ion Concentration; Hydroxysteroid Dehydrogenases; Iodoacetamide; Kinetics; Membranes; Microscopy, Electron; Microsomes; Mitochondria; Uterine Neoplasms | 1976 |
Differential reactivity of the two active site cysteine residues generated on reduction of pig heart lipoamide dehydrogenase.
Topics: Anaerobiosis; Animals; Binding Sites; Cysteine; Dihydrolipoamide Dehydrogenase; Dithionite; Hydrogen-Ion Concentration; Iodoacetamide; Myocardium; NAD; Oxidation-Reduction; Protein Binding; Protein Conformation; Spectrophotometry; Spectrophotometry, Ultraviolet; Swine | 1976 |
Cathepsin D activity in isolated odontoblasts.
Topics: Animals; Cathepsins; Cysteine; Hot Temperature; Hydrogen-Ion Concentration; Incisor; Iodoacetamide; Kinetics; Male; Odontoblasts; Pepstatins; Rats; Temperature | 1977 |
Reaction of yeast fatty acid synthetase with iodoacetamide. 1. Kinetics of inactivation and extent of carboxamidomethylation.
Topics: Cysteine; Ethylmaleimide; Fatty Acid Synthases; Hydrogen-Ion Concentration; Iodoacetamide; Iodoacetates; Kinetics; Saccharomyces cerevisiae; Sulfhydryl Reagents | 1977 |
Mechanism of porphobilinogen synthase. Possible role of essential thiol groups.
Topics: Alkylation; Cysteine; Hydrogen-Ion Concentration; Iodoacetamide; Iodoacetates; Levulinic Acids; Liver; Models, Biological; Porphobilinogen Synthase; Sulfhydryl Compounds | 1977 |
Activation of fungal alpha-amylase by dithioerythritol.
Topics: alpha-Amylases; Amylases; Aspergillus oryzae; Cell-Free System; Cysteine; Dithioerythritol; Dithiothreitol; Enzyme Activation; Iodoacetamide; Mercaptoethanol | 1979 |
Non-cooperative response of actin-cystein 373 in cooperatively behaving regulated actin filaments.
Topics: Actins; Adenosine Triphosphatases; Animals; Cysteine; Dithiothreitol; Ethylmaleimide; Fluorescent Dyes; Iodoacetamide; Kinetics; Magnesium; Naphthalenesulfonates; Protein Binding; Spectrometry, Fluorescence | 1979 |
The specificity of induced conformational changes. The case of yeast glyceraldehyde-3-phosphate dehydrogenase.
Topics: Benzoates; Binding Sites; Cysteine; Glyceraldehyde-3-Phosphate Dehydrogenases; Iodoacetamide; Kinetics; NAD; Nitriles; Protein Binding; Protein Conformation; Saccharomyces cerevisiae; Thiocyanates | 1975 |
Effect of carbamidomethylation of cysteine residues G11(104)alpha on the properties of hemoglobin A.
Topics: Binding Sites; Cysteine; Electron Spin Resonance Spectroscopy; Electrophoresis, Disc; Hemoglobins; Humans; Iodoacetamide; Macromolecular Substances; Oxygen; Oxyhemoglobins; Protein Binding; Protein Conformation | 1976 |
Essential and nonessential thiols of yeast hexokinase. Reactions with iodoacetate and iodoacetamide.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Alkylation; Cysteine; Electrophoresis, Polyacrylamide Gel; Glucose; Hexokinase; Hydrogen-Ion Concentration; Iodoacetamide; Iodoacetates; Isoenzymes; Kinetics; Macromolecular Substances; Magnesium; Osmolar Concentration; Saccharomyces cerevisiae; Sulfhydryl Compounds | 1975 |
Chemical modification of bovine heart mitochondrial malate dehydrogenase. Selective modification of cysteine and histidine.
Topics: Amino Acids; Animals; Carbon Radioisotopes; Cattle; Cysteine; Ethylmaleimide; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Iodoacetates; Malate Dehydrogenase; Malates; Mitochondria, Muscle; Molecular Weight; Myocardium; NAD; Oxaloacetates; Urea | 1975 |
The interaction of an epoxide with yeast alcohol dehydrogenase: evidence for binding and the modification of two active site cysteines by styrene oxide.
Topics: Alcohol Oxidoreductases; Binding Sites; Cysteine; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Mathematics; Protein Binding; Saccharomyces cerevisiae; Spectrophotometry, Ultraviolet; Styrenes; Time Factors; Trypsin | 1975 |
Interaction of streptococcal aggregating factors with thiol-and disulphide-reactive compounds.
Topics: Agglutination; Cysteine; Ferricyanides; Humans; Iodoacetamide; Saliva; Species Specificity; Streptococcus; Streptococcus sanguis; Sulfhydryl Reagents | 1977 |
Identification of the globin chain and the amino acid residue responsible for polymerization of bullfrog (Rana catesbeiana) hemoglobin with iodo[14C]acetamide.
Topics: Animals; Anura; Cysteine; Globins; Hemoglobins; Iodoacetamide; Iodoacetates; Macromolecular Substances; Protein Binding; Rana catesbeiana | 1978 |
Modification of the cysteine residues of the lactose repressor protein using chromophoric probes.
Topics: Acetamides; Bacterial Proteins; Binding Sites; Cysteine; Escherichia coli; Iodoacetamide; Kinetics; Lactose; Nitrophenols; Peptide Fragments; Protein Binding | 1977 |
Evidence for a single essential thiol in the yeast hexokinase molecule.
Topics: Acetylgalactosamine; Affinity Labels; Cysteine; Dithionitrobenzoic Acid; Hexokinase; Iodoacetamide; Iodoacetates; Saccharomyces cerevisiae; Thermodynamics; Thiocyanates | 1977 |
Reaction of yeast fatty acid synthetase with iodoacetamide. 2. Identification of the amino acid residues reacting with iodoacetamide and primary structure of a peptide containing the peripheral sulfhydryl group.
Topics: Acetyl Coenzyme A; Amino Acid Sequence; Cysteine; Fatty Acid Synthases; Iodoacetamide; Iodoacetates; Pepsin A; Peptide Fragments; Saccharomyces cerevisiae; Trypsin | 1977 |
The reactivity of sulfhydryl groups at the active site of an F-factor--specified variant of chloramphenicol acetyltransferase.
Topics: Acetyltransferases; Binding Sites; Chloramphenicol; Cysteine; Escherichia coli; Genetic Variation; Iodoacetamide; Kinetics; R Factors | 1978 |
Study of actin and its interactions with heavy meromyosin and the regulatory proteins by the pulse fluorimetry in polarized light of a fluorescent probe attached to an actin cysteine.
Topics: Actins; Animals; Binding Sites; Cysteine; Ethylenediamines; Fluorescent Dyes; Iodoacetamide; Kinetics; Mathematics; Muscle Proteins; Muscles; Myosin Subfragments; Protein Binding; Rabbits; Spectrometry, Fluorescence | 1978 |
lac repressor changes conformation upon binding to poly[dA-T)].
Topics: Cysteine; Escherichia coli; Fluorescent Dyes; Iodoacetamide; Kinetics; Naphthalenesulfonates; Peptide Fragments; Poly dA-dT; Polydeoxyribonucleotides; Protein Conformation; Repressor Proteins; Spectrometry, Fluorescence; Transcription Factors | 1979 |
Effect of some--SH and other reagents on aspartate aminotransferase and L-alanine aminotransferase of Paramphistomum explanatum Fischoeder, 1901.
Topics: Alanine Transaminase; Animals; Aspartate Aminotransferases; Cadmium; Cysteine; Edetic Acid; Iodoacetamide; Mercaptoethanol; Mercury; Silver; Sulfhydryl Reagents; Trematoda | 1978 |
Study of tropomyosin labelled with a fluorescent probe by pulse fluorimetry in polarized light. Interaction of that protein with troponin and actin.
Topics: Actins; Animals; Binding Sites; Cysteine; Ethylenediamines; Fluorescent Dyes; Iodoacetamide; Macromolecular Substances; Muscle Proteins; Muscles; Protein Binding; Rabbits; Spectrometry, Fluorescence; Tropomyosin; Troponin | 1978 |
Effects of cytochalasin A on the morphology of plasmodia and sclerotia of Physarum polycephalum.
Topics: Cysteine; Cytochalasins; Iodoacetamide; Mercaptoethanol; Physarum; Stereoisomerism | 1978 |
Reactivity of the cysteine and tyrosine residues of aspartate transaminase from chicken heart cytosol.
Topics: Animals; Aspartate Aminotransferases; Binding Sites; Chickens; Cysteine; Cytosol; Disulfides; Dithionitrobenzoic Acid; Ethylmaleimide; Iodoacetamide; Kinetics; Mercuribenzoates; Myocardium; Protein Binding; Pyridines; Sulfhydryl Compounds; Tetranitromethane; Tyrosine | 1975 |
A type kappa Bence Jones protein containing a cysteinyl residue in the variable region.
Topics: Alkylation; Amino Acids; Ammonium Sulfate; Bence Jones Protein; Chromatography, Ion Exchange; Circular Dichroism; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin Fragments; Iodoacetamide; Iodoacetates; Molecular Weight; Multiple Myeloma; Nitrobenzoates; Sulfhydryl Compounds | 1975 |
Comparison of cobalamin-independent and cobalamin-dependent methionine synthases from Escherichia coli: two solutions to the same chemical problem.
Topics: 5-Methyltetrahydrofolate-Homocysteine S-Methyltransferase; Alkylation; Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cysteine; Escherichia coli; Homocysteine; Iodoacetamide; Methionine; Molecular Sequence Data; Recombinant Proteins; Restriction Mapping; Tetrahydrofolates; Tosyllysine Chloromethyl Ketone; Tosylphenylalanyl Chloromethyl Ketone; Trypsin; Vitamin B 12 | 1992 |
Effect of introducing different carboxylate-containing side chains at position 85 on chromophore formation and proton transport in bacteriorhodopsin.
Topics: Amino Acid Sequence; Aspartic Acid; Bacteriorhodopsins; Cysteine; Glutamates; Glutamic Acid; Halobacterium salinarum; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Kinetics; Mutagenesis, Site-Directed; Protons; Recombinant Proteins | 1992 |
Site-directed mutagenesis of the active site cysteine in Klebsiella aerogenes urease.
Topics: Base Sequence; Binding Sites; Cysteine; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Klebsiella pneumoniae; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligonucleotides; Peptide Mapping; Urease | 1992 |
Thermal motions of surface alpha-helices in the D-galactose chemosensory receptor. Detection by disulfide trapping.
Topics: Calcium-Binding Proteins; Carrier Proteins; Chemotaxis; Cysteine; Disulfides; Escherichia coli; Galactose; Hot Temperature; Iodoacetamide; Kinetics; Magnetic Resonance Spectroscopy; Models, Chemical; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Periplasmic Binding Proteins; Protein Conformation; Protein Denaturation; Protein Engineering; Surface Properties; Tryptophan | 1992 |
Characterization of functional HPV-16 E7 protein produced in Escherichia coli.
Topics: Amino Acid Sequence; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genetic Vectors; Iodoacetamide; Molecular Sequence Data; Oncogene Proteins, Viral; Papillomavirus E7 Proteins; Retinoblastoma Protein; Zinc | 1992 |
The cysteine residues in the carboxy terminal domain of tropoelastin form an intrachain disulfide bond that stabilizes a loop structure and positively charged pocket.
Topics: Amino Acid Sequence; Animals; Cattle; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithionitrobenzoic Acid; Drug Stability; Elastin; Electrophoresis, Polyacrylamide Gel; Humans; Iodoacetamide; Molecular Sequence Data; Peptide Fragments; Protein Conformation; Sequence Homology, Nucleic Acid; Tropoelastin | 1992 |
Covalent modification and active site-directed inactivation of a low molecular weight phosphotyrosyl protein phosphatase.
Topics: Animals; Arginine; Arsenicals; Binding Sites; Binding, Competitive; Cattle; Cyclohexanones; Cysteine; Epoxy Compounds; Glutathione; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Kinetics; Molecular Weight; Phenylglyoxal; Protein Tyrosine Phosphatases | 1992 |
The type I and type II bovine scavenger receptors expressed in Chinese hamster ovary cells are trimeric proteins with collagenous triple helical domains comprising noncovalently associated monomers and Cys83-disulfide-linked dimers.
Topics: 2,2'-Dipyridyl; Amino Acid Sequence; Animals; Base Sequence; Cattle; CHO Cells; Cricetinae; Cysteine; Disulfides; Iodoacetamide; Kinetics; Macromolecular Substances; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Receptors, Immunologic; Receptors, Lipoprotein; Receptors, Scavenger; Recombinant Proteins; Scavenger Receptors, Class B; Transfection; Tunicamycin | 1991 |
Sulphydryl mediation in the protection of gastric mucosal cells in tissue culture by acetaminophen.
Topics: Acetaminophen; Cell Line; Cell Survival; Cysteine; Gastric Mucosa; Glutathione; Humans; In Vitro Techniques; Iodoacetamide; Sulfhydryl Compounds; Taurocholic Acid | 1991 |
Chemical modification of PABA synthase.
Topics: Arginine; Cysteine; Diethyl Pyrocarbonate; Escherichia coli; Folic Acid; Histidine; Iodoacetamide; Phenylglyoxal; Sulfhydryl Reagents; Tetranitromethane; Transaminases; Tyrosine | 1991 |
Cysteine-86 is not needed for the enzymic activity of glutathione S-transferase 3-3.
Topics: Amino Acid Sequence; Baculoviridae; Base Sequence; Cloning, Molecular; Cysteine; DNA; Glutathione Transferase; Iodoacetamide; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Recombinant Proteins; Serine Endopeptidases; Structure-Activity Relationship; Transfection | 1991 |
The single cysteine residue on an alpha family chick liver glutathione S-transferase CL 3-3 is not functionally important.
Topics: Amino Acid Sequence; Animals; Chickens; Chromatography, High Pressure Liquid; Cysteine; Gene Expression; Glutathione Transferase; Iodoacetamide; Liver; Molecular Sequence Data; Peptide Fragments; Recombinant Proteins; Structure-Activity Relationship; Substrate Specificity | 1991 |
Cysteine residues in the active site of Corynebacterium sarcosine oxidase.
Topics: Acetates; Amino Acid Sequence; Amino Acids; Binding Sites; Chromatography, High Pressure Liquid; Corynebacterium; Cyanogen Bromide; Cysteine; Iodoacetamide; Isotope Labeling; Molecular Sequence Data; Oxidation-Reduction; Oxidoreductases, N-Demethylating; Peptides; Sarcosine Oxidase; Sulfites | 1991 |
Evidence for reactivity of serine-74 with trans-4-(N,N-dimethylamino)cinnamaldehyde during oxidation by the cytoplasmic aldehyde dehydrogenase from sheep liver.
Topics: Aldehyde Dehydrogenase; Amino Acid Sequence; Animals; Binding Sites; Cinnamates; Cysteine; Cytoplasm; Iodoacetamide; Liver; Molecular Sequence Data; Serine; Sheep; Thermolysin | 1990 |
Isolation and characterization of acetylated histones H3 and H4 and their assembly into nucleosomes.
Topics: Acetylation; Binding Sites; Cell Nucleus; Chromatography, High Pressure Liquid; Cysteine; Deoxyribonuclease I; DNA; Electrophoresis, Polyacrylamide Gel; HeLa Cells; Histones; Humans; Iodoacetamide; Lysine; Nucleosomes | 1990 |
Sulfhydryl chemistry of Salmonella typhimurium phosphoribosylpyrophosphate synthetase: identification of two classes of cysteinyl residues.
Topics: Amino Acid Sequence; Binding Sites; Chromatography, High Pressure Liquid; Cyanogen Bromide; Cysteine; Dithionitrobenzoic Acid; Iodoacetamide; Kinetics; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Phosphotransferases; Ribose-Phosphate Pyrophosphokinase; Salmonella typhimurium; Sulfhydryl Reagents | 1990 |
Free thiols of platelet thrombospondin. Evidence for disulfide isomerization.
Topics: Amino Acid Sequence; Blood Platelets; Cysteine; Disulfides; Ethylmaleimide; Humans; Iodoacetamide; Isomerism; Kinetics; Molecular Sequence Data; Platelet Membrane Glycoproteins; Sequence Homology, Nucleic Acid; Thrombospondins | 1990 |
Elimination of 14C by calves, pigs and rats renally perfused with radiolabelled dichlorovinyl or acetamide substrates.
Topics: Acetanilides; Acetylation; Acetylcysteine; Animals; Carbon Radioisotopes; Cattle; Cysteine; Herbicides; Iodoacetamide; Kidney; Perfusion; Rats; Rats, Inbred Strains; Swine; Trichloroethylene | 1990 |
The redox state of cysteines 201 and 317 of the erythrocyte anion exchanger is critical for ankyrin binding.
Topics: 4-Chloromercuribenzenesulfonate; Anion Exchange Protein 1, Erythrocyte; Anion Transport Proteins; Ankyrins; Blood Proteins; Carrier Proteins; Cysteine; Erythrocyte Membrane; Ethylmaleimide; Glutathione; Humans; Iodoacetamide; Kinetics; Membrane Proteins; Molecular Weight; Oxidation-Reduction; Phenanthrolines | 1989 |
Conformational analysis of thioredoxin using organoarsenical reagents as probes. A time-resolved fluorescence anisotropy and size exclusion chromatography study.
Topics: Alkylation; Arsenic; Bacterial Proteins; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Electrophoresis, Polyacrylamide Gel; Fluorescence Polarization; Indicators and Reagents; Iodoacetamide; Molecular Weight; Organometallic Compounds; Oxidation-Reduction; Protein Conformation; Scintillation Counting; Thioctic Acid; Thioredoxins | 1989 |
Binding site of cerulenin in fatty acid synthetase.
Topics: Amino Acids; Antifungal Agents; Binding Sites; Cerulenin; Chromatography, High Pressure Liquid; Cysteine; Fatty Acid Synthases; Hydrolysis; Iodoacetamide; Magnetic Resonance Spectroscopy; Saccharomyces cerevisiae; Trypsin | 1989 |
Transcriptional switching by the metalloregulatory MerR protein: initial characterization of DNA and mercury (II) binding activities.
Topics: Alkylation; Bacterial Proteins; Base Sequence; Chromatography, High Pressure Liquid; Cysteine; Deoxyribonuclease I; Dithionitrobenzoic Acid; DNA-Binding Proteins; DNA, Bacterial; Gene Expression Regulation; Iodoacetamide; Kinetics; Ligands; Mercury; Metalloproteins; Methylation; Mutation; Operator Regions, Genetic; Promoter Regions, Genetic; Protein Binding; Thermodynamics; Transcription, Genetic | 1989 |
Cadmium binding and metal cluster formation in metallothionein: a differential modification study.
Topics: Animals; Binding Sites; Cadmium; Chemical Phenomena; Chemistry; Cysteine; Iodoacetamide; Metallothionein; Peptide Fragments; Trypsin | 1987 |
Transcription by T7 RNA polymerase is not zinc-dependent and is abolished on amidomethylation of cysteine-347.
Topics: Amino Acids; Chromatography, High Pressure Liquid; Cysteine; Edetic Acid; Escherichia coli; Iodoacetamide; Iodoacetates; Kinetics; Peptide Fragments; T-Phages; Transcription, Genetic; Trypsin; Zinc | 1986 |
Effects of Ca2+ and subunit interactions on surface accessibility of cysteine residues in cardiac troponin.
Topics: Animals; Binding Sites; Calcium; Cattle; Chromatography, High Pressure Liquid; Cysteine; In Vitro Techniques; Iodoacetamide; Myocardium; Peptide Fragments; Protein Conformation; Rabbits; Troponin | 1988 |
Characterization of the third component of complement (C3) after activation by cigarette smoke.
Topics: Chemical Phenomena; Chemistry; Complement Activation; Complement C3; Complement Factor B; Complement Pathway, Alternative; Cysteine; Humans; Iodoacetamide; Methylamines; Molecular Weight; Nicotiana; Plants, Toxic; Smoke | 1987 |
Purification and properties of thioltransferase.
Topics: Amino Acids; Animals; Cysteine; Cytoplasm; Electrophoresis, Polyacrylamide Gel; Glutaredoxins; Glutathione; Hydrogen-Ion Concentration; Iodoacetamide; Isoelectric Focusing; Kinetics; Liver; Molecular Weight; Oxidoreductases; Protein Disulfide Reductase (Glutathione); Rats | 1986 |
Modification of the calmodulin-stimulated phosphatase, calcineurin, by sulfhydryl reagents.
Topics: Animals; Binding Sites; Calcium; Calmodulin-Binding Proteins; Cattle; Cysteine; Dithionitrobenzoic Acid; Ethylmaleimide; Hydroxymercuribenzoates; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Kinetics; Macromolecular Substances; Manganese; Nickel; Sulfhydryl Reagents | 1986 |
Stoichiometry of substrate binding to rat liver fatty acid synthetase.
Topics: Acetates; Acetic Acid; Acetyl Coenzyme A; Animals; Binding Sites; Cysteamine; Cysteine; Fatty Acid Synthases; Iodoacetamide; Liver; Malonates; Malonyl Coenzyme A; Pantetheine; Rats | 1985 |
Two sulfhydryl groups near the active site of thiolase I from porcine heart: modification of thiolase with the fluorescent thiol reagent S-mercurio-N-dansyl-L-cysteine.
Topics: Acetyl-CoA C-Acetyltransferase; Animals; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Iodoacetamide; Kinetics; Myocardium; Spectrometry, Fluorescence; Sulfhydryl Reagents; Swine | 1982 |
Sulfhydryl groups of the F1 adenosine triphosphatase of Escherichia coli and the stoichiometry of the subunits.
Topics: Affinity Labels; Cysteine; Escherichia coli; Ethylmaleimide; Iodoacetamide; Isoelectric Focusing; Macromolecular Substances; Proton-Translocating ATPases; Sulfhydryl Compounds | 1984 |
Regeneration of reduced-denatured seminal ribonuclease: effect of modification at cysteines 31 and 32.
Topics: Animals; Aziridines; Cattle; Cysteine; Dithiothreitol; Endonucleases; Glutathione; Iodoacetamide; Iodoacetates; Kinetics; Male; Maleimides; Oxidation-Reduction; Protein Denaturation; Ribonuclease, Pancreatic; Ribonucleases; Semen; Temperature | 1980 |
Localization of cysteine115 in subunit III of beef heart cytochrome C oxidase to the C side of the mitochondrial inner membrane.
Topics: Amino Acids; Animals; Binding Sites; Cattle; Cyanogen Bromide; Cysteine; Electron Transport Complex IV; Intracellular Membranes; Iodoacetamide; Macromolecular Substances; Mitochondria, Heart; Nitrobenzoates; Peptide Fragments; Sulfhydryl Compounds | 1982 |
lac Repressor cysteine-140 reacts selectively with a fluorescent probe bound to the core-headpiece interface.
Topics: Cysteine; Escherichia coli; Iodoacetamide; Naphthalenesulfonates; Repressor Proteins; Transcription Factors; Trypsin | 1984 |
Chorismate mutase/prephenate dehydrogenase from Escherichia coli K12: purification, characterization, and identification of a reactive cysteine.
Topics: Amino Acid Sequence; Centrifugation; Chemical Phenomena; Chemistry; Chorismate Mutase; Chromatography, Affinity; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Iodoacetamide; Isomerases; Kinetics; Molecular Weight; Oxidoreductases; Peptide Fragments; Prephenate Dehydrogenase; Thermolysin; Trypsin | 1984 |
The role of cysteinyl residues in the phosphorylase kinase activity as revealed by iodacetamide modification.
Topics: Animals; Binding Sites; Carbon Radioisotopes; Cysteine; Iodoacetamide; Iodoacetates; Kinetics; Muscles; Phosphorylase b; Phosphorylase Kinase; Rabbits | 1983 |
Hybridization studies of chicken liver fatty acid synthetase. Evidence for the participation in palmitate synthesis of cysteine and phosphopantetheine sulfhydryl groups on adjacent subunits.
Topics: Acetyl Coenzyme A; Animals; Chickens; Cysteine; Fatty Acid Synthases; Iodoacetamide; Liver; Palmitates; Palmitic Acids; Sulfhydryl Compounds; Time Factors | 1984 |
Role of thiols, pH and cathepsin D in the lysosomal catabolism of serum albumin.
Topics: Animals; Cathepsin D; Cathepsins; Cysteine; Glutathione; Hydrogen-Ion Concentration; In Vitro Techniques; Iodoacetamide; Kidney; Leupeptins; Liver; Lysosomes; Mice; Pepstatins; Rats; Rats, Inbred Strains; Serum Albumin, Bovine; Sulfhydryl Compounds | 1984 |
Chemical modification of Cys-374 of actin interferes with the formation of the profilactin complex.
Topics: Actins; Animals; Cattle; Contractile Proteins; Cysteine; Ethylmaleimide; Fluorescent Dyes; Iodoacetamide; Iodoacetates; Macromolecular Substances; Microfilament Proteins; Profilins; Proteins; Spectrometry, Fluorescence; Spleen | 1984 |
Inactivation of 3-oxoacyl synthetase activity of pigeon liver fatty acid synthetase by S-(4-bromo-2,3-dioxobutyl)-coenzyme A.
Topics: 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; Acetyl Coenzyme A; Acetyltransferases; Acyl-Carrier Protein S-Acetyltransferase; Acyltransferases; Amino Acids; Animals; Coenzyme A; Columbidae; Cysteine; Enzyme Activation; Fatty Acid Synthases; Iodoacetamide; Kinetics; Liver; Malonyl Coenzyme A; Pantetheine; Spectrophotometry | 1983 |
The yeast fatty acid synthetase. Structure-function relationship and the role of the active cysteine-SH and pantetheine-SH.
Topics: Cysteine; Fatty Acid Synthases; Hydrocarbons, Brominated; Iodoacetamide; Kinetics; Models, Molecular; Pantetheine; Propane; Protein Conformation; Saccharomyces cerevisiae; Sulfhydryl Compounds | 1981 |
Chicken liver fatty acid synthetase dimer: evidence of asymmetrical structure from iodoacetamide inhibition studies.
Topics: Acetyl Coenzyme A; Animals; Chickens; Cysteine; Fatty Acid Synthases; Iodoacetamide; Iodoacetates; Kinetics; Liver; Macromolecular Substances; Pantetheine; Protein Conformation | 1982 |
Effect of histone H3 sulfhydryl modifications on histone-histone interactions and nucleosome formation and structure.
Topics: Animals; Cattle; Chickens; Cysteine; Disulfides; Fluoresceins; Guanidines; Histones; Iodoacetamide; Iodoacetates; Macromolecular Substances; Naphthalenesulfonates; Nucleosomes; Protein Binding; Protein Denaturation; Sulfhydryl Compounds; Thymus Gland | 1980 |
Ligand-dependent allosteric transformation of hemoglobins from the blood clam, Anadara broughtonii.
Topics: Allosteric Regulation; Animals; Bivalvia; Chloromercuribenzoates; Cysteine; Hemoglobins; Iodoacetamide; Ligands; Oxygen; Protein Conformation | 1980 |
The role of cysteine-949 in the binding of transforming growth factor-beta 1 and transforming growth factor-beta 2 to alpha 2-macroglobulin.
Topics: alpha-Macroglobulins; Cysteine; Disulfides; Humans; In Vitro Techniques; Iodoacetamide; Kinetics; Transforming Growth Factor beta | 1994 |
Accessibility and dynamics of Cys residues in Bacteriophage IKe and M13 major coat protein mutants.
Topics: Alkylation; Amino Acid Sequence; Bacteriophage M13; Capsid; Capsid Proteins; Cysteine; Electron Spin Resonance Spectroscopy; Inovirus; Iodoacetamide; Membrane Proteins; Micelles; Molecular Probes; Molecular Sequence Data; Mutation; Protein Conformation; Sequence Homology, Amino Acid; Solubility; Spectrometry, Fluorescence; Spin Labels; Sulfhydryl Reagents; Virion | 1995 |
Identification of the IMP binding site in the IMP dehydrogenase from Tritrichomonas foetus.
Topics: Animals; Base Sequence; Binding Sites; Chromatography, High Pressure Liquid; Cysteine; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; IMP Dehydrogenase; Inosine Monophosphate; Iodoacetamide; Kinetics; Molecular Sequence Data; Recombinant Proteins; Substrate Specificity; Thionucleotides; Tritrichomonas foetus; Trypsin | 1995 |
Locations of disulfide bonds and free cysteines in the heavy and light chains of recombinant human factor VIII (antihemophilic factor A).
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Factor VIII; Humans; Iodoacetamide; Molecular Sequence Data; Peptides; Protein Denaturation; Recombinant Proteins; Sequence Analysis | 1995 |
Effects of D-penicillamine on multiple immunogenic epitopes of the acetylcholine receptor.
Topics: Animals; Antibodies, Monoclonal; Antigen-Antibody Reactions; Cysteine; Epitopes; In Vitro Techniques; Iodoacetamide; Penicillamine; Receptors, Nicotinic; Torpedo | 1993 |
Endogenous, Ca(2+)-dependent cysteine-protease cleaves specifically the ryanodine receptor/Ca2+ release channel in skeletal muscle.
Topics: Adenosine Triphosphate; Amino Acid Sequence; Animals; Calcium; Calcium Channels; Calpain; Cysteine; Dithiothreitol; Iodoacetamide; Leupeptins; Magnesium; Mercaptoethanol; Mercuric Chloride; Molecular Sequence Data; Molecular Weight; Muscle Proteins; Muscle, Skeletal; Rabbits; Ryanodine Receptor Calcium Release Channel; Sarcoplasmic Reticulum; Sodium Chloride; Substrate Specificity | 1994 |
Identification of the reactive sulfhydryl group of 1-aminocyclopropane-1-carboxylate deaminase.
Topics: Alanine; Amino Acid Sequence; Amino Acids; Amino Acids, Cyclic; Carbon-Carbon Lyases; Cysteine; Iodoacetamide; Lyases; Molecular Sequence Data; Naphthalenesulfonates; Protein Conformation; Pseudomonas; Spectrophotometry, Ultraviolet; Temperature | 1993 |
Modification of cysteine residues with N-methyl iodoacetamide.
Topics: Amino Acids; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Hirudins; Indicators and Reagents; Iodoacetamide; Phenylthiohydantoin; Proteinase Inhibitory Proteins, Secretory; Proteins; Serine Proteinase Inhibitors | 1994 |
Local environmental change from the G- to F-form of the actin molecule detected on anisotropy decay measurement.
Topics: Actins; Cysteine; Fluorescence Polarization; Fluorescent Dyes; Iodoacetamide; Protein Conformation | 1994 |
Modification of cysteine residues within G(o) and other neuronal proteins by exposure to nitric oxide.
Topics: Animals; Cattle; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; GTP-Binding Proteins; In Vitro Techniques; Iodoacetamide; Mercury; Nerve Tissue Proteins; Nitric Oxide; Pertussis Toxin; Protein Binding; Rats; Synaptic Membranes; Synaptosomes; Virulence Factors, Bordetella | 1994 |
Transglutaminase inhibition by 2-[(2-oxopropyl)thio]imidazolium derivatives: mechanism of factor XIIIa inactivation.
Topics: Animals; Binding Sites; Cysteine; Dogs; Erythrocytes; Glutathione; Humans; Hydrogen-Ion Concentration; Imidazoles; Iodoacetamide; Kinetics; Magnetic Resonance Spectroscopy; Molecular Weight; Sulfhydryl Compounds; Transglutaminases | 1994 |
The cooperative binding of chromosomal protein HMG-14 to nucleosome cores is reduced by single point mutations in the nucleosomal binding domain.
Topics: Alanine; Base Sequence; Binding Sites; Cysteine; Escherichia coli; Gene Expression; Glutamic Acid; High Mobility Group Proteins; Humans; Iodoacetamide; Lysine; Molecular Sequence Data; Mutagenesis, Site-Directed; Nucleosomes; Point Mutation; Protein Conformation; Recombinant Proteins; Structure-Activity Relationship; Sulfhydryl Compounds | 1994 |
Synthetic glycosylation of proteins using N-(beta-saccharide) iodoacetamides: applications in site-specific glycosylation and solid-phase enzymic oligosaccharide synthesis.
Topics: Alkylation; Amino Acid Sequence; Carbohydrate Sequence; Cysteine; Glycoproteins; Glycosylation; Iodoacetamide; Molecular Sequence Data; Oligosaccharides; Peptides; Serum Albumin, Bovine | 1994 |
Inactivation of a cytosolic phospholipase A2 by thiol-modifying reagents: cysteine residues as potential targets of phospholipase A2.
Topics: Amino Acid Sequence; Cell Line; Cysteine; Cytosol; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Stability; Humans; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Molecular Sequence Data; Phospholipases A; Phospholipases A2; Recombinant Proteins; Sulfhydryl Reagents | 1994 |
Aldehyde dehydrogenase: aldehyde dehydrogenation and ester hydrolysis.
Topics: Aldehyde Dehydrogenase; Aldehydes; Binding Sites; Cinnamates; Cysteine; Esters; Glutamates; Glutamic Acid; Humans; Hydrolysis; Imidazoles; In Vitro Techniques; Iodoacetamide; Isoenzymes; Kinetics; Liver; Substrate Specificity | 1993 |
Site-directed mutagenesis of human tissue transglutaminase: Cys-277 is essential for transglutaminase activity but not for GTPase activity.
Topics: Binding Sites; Cysteine; Dithiothreitol; Erythrocytes; GTP Phosphohydrolases; Humans; Iodoacetamide; Mutagenesis, Site-Directed; Recombinant Proteins; Serine; Transglutaminases | 1993 |
Replacement of the active-site cysteine residues of DsbA, a protein required for disulfide bond formation in vivo.
Topics: Base Sequence; Binding Sites; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Glutathione; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Isomerases; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Polymerase Chain Reaction; Protein Conformation; Protein Disulfide-Isomerases; Serine; Thermodynamics | 1994 |
Selective chemical modification of Cys264 with diiodofluorescein iodacetamide as a tool to study the membrane topology of cytochrome P450scc (CYP11A1).
Topics: Adrenodoxin; Amino Acid Sequence; Animals; Cattle; Cell Membrane; Cholesterol Side-Chain Cleavage Enzyme; Cysteine; Fluoresceins; Fluorescent Dyes; Iodoacetamide; Liposomes; Molecular Sequence Data; Trypsin | 1994 |
The pH-dependent behavior of catalytic activities of Azospirillum brasilense glutamate synthase and iodoacetamide modification of the enzyme provide evidence for a catalytic Cys-His ion pair.
Topics: Amino Acid Sequence; Ammonia; Azospirillum brasilense; Catalysis; Cysteine; Glutamate Synthase; Glutamine; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Ketoglutaric Acids; Kinetics; Molecular Sequence Data; NADP; Sequence Analysis | 1994 |
Reactivity and ionization of the active site cysteine residues of DsbA, a protein required for disulfide bond formation in vivo.
Topics: Amino Acid Sequence; Binding Sites; Cysteine; Disulfides; Glutathione; Hydrogen-Ion Concentration; Iodoacetamide; Ions; Isomerases; Kinetics; Models, Chemical; Molecular Sequence Data; Protein Disulfide-Isomerases; Spectrophotometry, Ultraviolet | 1994 |
Interaction of ethacrynic acid with bovine brain tubulin.
Topics: Alkylation; Animals; Brain; Cattle; Cysteine; Ethacrynic Acid; Iodoacetamide; Microtubules; Tubulin | 1994 |
Identification of reactive vicinal cysteines in Saccharomyces cerevisiae (ATP) and cytosolic rat liver (GTP) phospho enol pyruvate carboxykinases.
Topics: Adenosine Triphosphate; Amino Acid Sequence; Animals; Cysteine; Cytosol; Dithionitrobenzoic Acid; Fluorescent Dyes; Glucosides; Guanosine Triphosphate; Iodoacetamide; Liver; Phosphoenolpyruvate Carboxykinase (GTP); Pyrimidinones; Rats; Saccharomyces cerevisiae | 1993 |
Disulfide arrangement and chemical modification of beta-1,4-endoglucanase E2 from Thermomonospora fusca.
Topics: Actinomycetales; Amino Acid Sequence; Cellobiose; Cellulase; Cellulose; Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Ethyldimethylaminopropyl Carbodiimide; Hot Temperature; Iodoacetamide; Methylation; Molecular Sequence Data; Oxidation-Reduction; Protein Denaturation | 1993 |
Disulfide arrangement and functional domains of beta-1,4-endoglucanse E5 from Thermomonospora fusca.
Topics: Actinomycetales; Amino Acid Sequence; Binding Sites; Cellulase; Cellulose; Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Iodoacetamide; Methylation; Molecular Sequence Data; Oxidation-Reduction; Peptide Fragments; Sulfhydryl Compounds | 1993 |
G protein beta gamma subunit: physical and chemical characterization.
Topics: Carbon Radioisotopes; Cross-Linking Reagents; Cysteine; GTP-Binding Proteins; Hot Temperature; Iodoacetamide; Kinetics; Macromolecular Substances; Maleimides; Molecular Weight; NAD; Peptide Fragments; Phosphorus Radioisotopes; Protein Denaturation; Thermodynamics; Trypsin; Virulence Factors, Bordetella | 1993 |
Peculiar spectroscopic and kinetic properties of Cys-47 in human placental glutathione transferase. Evidence for an atypical thiolate ion pair near the active site.
Topics: Alkylation; Binding Sites; Chemical Phenomena; Chemistry, Physical; Cysteine; Electrochemistry; Female; Glutathione; Glutathione Transferase; Humans; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Models, Molecular; Placenta; Pyruvates; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; X-Ray Diffraction | 1993 |
Disruption of the cysteine-75 and zinc ion coordination is not sufficient to activate the precursor of human matrix metalloproteinase 3 (stromelysin 1).
Topics: Alkylation; Amino Acid Sequence; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Edetic Acid; Enzyme Activation; Humans; Iodoacetamide; Matrix Metalloproteinase 3; Mercuric Chloride; Metalloendopeptidases; Molecular Sequence Data; Phenylmercuric Acetate; Sulfhydryl Reagents; Zinc | 1993 |
The pKa of the catalytic histidine residue of chloramphenicol acetyltransferase.
Topics: Benzenesulfonates; Binding Sites; Catalysis; Chloramphenicol O-Acetyltransferase; Cysteine; Escherichia coli; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics | 1993 |
N-isopropyliodoacetamide in the reduction and alkylation of proteins: use in microsequence analysis.
Topics: Alkylation; Chromatography, High Pressure Liquid; Cysteine; Indicators and Reagents; Iodoacetamide; Microchemistry; Oxidation-Reduction; Peptide Mapping; Phenylthiohydantoin; Proteins; Sequence Analysis; Sulfhydryl Compounds | 1993 |
The reactive and destabilizing disulfide bond of DsbA, a protein required for protein disulfide bond formation in vivo.
Topics: Amino Acid Sequence; Bacterial Proteins; Circular Dichroism; Cysteine; Disulfides; Drug Stability; Escherichia coli; Glutathione; Iodoacetamide; Isomerases; Kinetics; Molecular Sequence Data; Oxidation-Reduction; Protein Disulfide-Isomerases; Protein Folding; Spectrometry, Fluorescence; Sulfhydryl Compounds; Thermodynamics | 1993 |
Determination of the disulfide bridges in factor Va light chain.
Topics: Amino Acid Sequence; Animals; Cattle; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Factor Va; Factor VIIIa; Humans; Iodoacetamide; Molecular Sequence Data; Peptide Fragments; Protein C; Sequence Homology, Amino Acid; Serine Endopeptidases; Trypsin | 1993 |
The inhibition mechanism of serpins. Evidence that the mobile reactive center loop is cleaved in the native protease-inhibitor complex.
Topics: Autoradiography; Binding Sites; Carbon Radioisotopes; Cloning, Molecular; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Iodoacetamide; Kinetics; Mutagenesis, Insertional; Plasminogen Activator Inhibitor 1; Point Mutation; Recombinant Proteins; Urokinase-Type Plasminogen Activator | 1995 |
Restoration of pore-forming activity in staphylococcal alpha-hemolysin by targeted covalent modification.
Topics: Animals; Bacterial Toxins; Cysteine; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Hemolysin Proteins; Histidine; Iodoacetamide; Mutagenesis, Site-Directed; Point Mutation; Porins; Protein Engineering; Rabbits; Staphylococcus; Temperature | 1995 |
Site-directed mutagenesis of Cys324 and Cys331 in human cytosolic phospholipase A2: locus of action of thiol modification reagents leading to inactiviation of cPLA2.
Topics: Amino Acid Sequence; Animals; Cells, Cultured; Cysteine; Cytosol; Humans; Iodoacetamide; Molecular Sequence Data; Mutagenesis, Site-Directed; Phospholipases A; Phospholipases A2; Structure-Activity Relationship; Sulfhydryl Reagents; Transfection | 1996 |
An active-site cysteine of sorghum leaf NADP-malate dehydrogenase studied by site-directed mutagenesis.
Topics: Base Sequence; Binding Sites; Cysteine; Enzyme Activation; Iodoacetamide; Kinetics; Malate Dehydrogenase; Malate Dehydrogenase (NADP+); Molecular Sequence Data; Molecular Weight; Mutagenesis, Site-Directed; Plant Leaves; Poaceae; Recombinant Fusion Proteins; Sulfhydryl Reagents | 1996 |
Inactivation of cholesteryl ester transfer protein by cysteine modification.
Topics: 4-Chloromercuribenzenesulfonate; Alkylating Agents; Animals; Azides; Carrier Proteins; Cell Line; Cholesterol Ester Transfer Proteins; Cholesterol Esters; Cricetinae; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Glycoproteins; Humans; Iodoacetamide; Kinetics; Pyridines; Recombinant Proteins; Structure-Activity Relationship; Sulfhydryl Reagents; Transfection | 1996 |
Steric constraints in the recognition of peptide substrates for the epidermal growth factor receptor kinase.
Topics: Binding Sites; Cysteine; Epidermal Growth Factor; ErbB Receptors; Fluoresceins; Fluorescent Dyes; Iodoacetamide; Kinetics; Mass Spectrometry; Molecular Weight; Oligopeptides; Phosphorylation; Protein Conformation; Signal Transduction; Substrate Specificity; Tyrosine | 1996 |
Fatty acylation of the rat and human asialoglycoprotein receptors. A conserved cytoplasmic cysteine residue is acylated in all receptor subunits.
Topics: Acylation; Animals; Asialoglycoprotein Receptor; Chromatography, High Pressure Liquid; Cysteine; Fatty Acids; Humans; Hydroxylamine; Hydroxylamines; Iodoacetamide; Molecular Weight; Palmitic Acid; Protein Conformation; Rats; Rats, Sprague-Dawley; Receptors, Cell Surface; Subtilisins | 1996 |
Chemical modification and site-directed mutagenesis of the single cysteine in motif 3 of class II Escherichia coli prolyl-tRNA synthetase.
Topics: Amino Acid Sequence; Amino Acyl-tRNA Synthetases; Binding Sites; Catalysis; Cysteine; Dithionitrobenzoic Acid; Enzyme Inhibitors; Escherichia coli; Iodoacetamide; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Naphthalenesulfonates; Point Mutation; RNA, Transfer, Amino Acyl; Sequence Alignment | 1997 |
[Selective chemical modification of cytochrome P450scc (CYP11A1) with diiodofluorescein iodoacetamide in the study of the role and topology of interdomain hinge of the hemoprotein molecule].
Topics: Adrenal Cortex; Adrenodoxin; Animals; Cattle; Cholesterol Side-Chain Cleavage Enzyme; Cysteine; Fluoresceins; Fluorescent Dyes; Iodoacetamide; Liposomes; Membrane Proteins; Proteolipids | 1996 |
A thioreduction pathway tethered to the membrane for periplasmic cytochromes c biogenesis; in vitro and in vivo studies.
Topics: Amino Acid Sequence; Bacterial Proteins; Cell Membrane; Cysteine; Cytochrome c Group; Dithiothreitol; Escherichia coli; Genes, Bacterial; Glutathione; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Lyases; Membrane Proteins; Molecular Sequence Data; Oxidation-Reduction; Protein Sorting Signals; Recombinant Fusion Proteins; Rhodobacter capsulatus; Sulfhydryl Reagents | 1997 |
Pyrene excimer fluorescence as a proximity probe for investigation of residual structure in the unfolded state of human carbonic anhydrase II.
Topics: Carbonic Anhydrases; Circular Dichroism; Computer Simulation; Cysteine; Fluorescent Dyes; Guanidine; Humans; Iodoacetamide; Models, Molecular; Protein Conformation; Protein Denaturation; Pyrenes; Spectrometry, Fluorescence | 1997 |
Inactivation of the polyketide synthase, 6-methylsalicylic acid synthase, by the specific modification of Cys-204 of the beta-ketoacyl synthase by the fungal mycotoxin cerulenin.
Topics: 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; Acetyl Coenzyme A; Acyltransferases; Alkylation; Antifungal Agents; Binding, Competitive; Cerulenin; Cyanogen Bromide; Cysteine; Iodoacetamide; Kinetics; Ligases; Malonyl Coenzyme A; Models, Chemical; Multienzyme Complexes; Oxidoreductases; Penicillium; Peptide Mapping; Serine Endopeptidases; Staphylococcus aureus | 1998 |
The switch in the helical handedness of the histone (H3-H4)2 tetramer within a nucleoprotein particle requires a reorientation of the H3-H3 interface.
Topics: Animals; Base Sequence; Cysteine; Dithionitrobenzoic Acid; DNA; DNA Primers; DNA, Circular; Ducks; Ethylmaleimide; Histones; Iodoacetamide; Kinetics; Macromolecular Substances; Mammary Tumor Virus, Mouse; Nucleoproteins; Protein Structure, Secondary; Repetitive Sequences, Nucleic Acid; Stress, Mechanical; Sulfhydryl Reagents | 1998 |
Chemical modifications of Bacillus subtilis tryptophanyl-tRNA synthetase.
Topics: Bacillus subtilis; Binding Sites; Bromosuccinimide; Cysteine; Enzyme Activation; Iodoacetamide; Protein Conformation; Skatole; Sulfhydryl Compounds; Tryptophan; Tryptophan-tRNA Ligase | 1997 |
Probing the role of cysteine residues in the EcoP15I DNA methyltransferase.
Topics: Amino Acid Substitution; Cloning, Molecular; Cysteine; Dithionitrobenzoic Acid; DNA Methylation; Escherichia coli; Ethylmaleimide; Iodoacetamide; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Protein Denaturation; Recombinant Proteins; Site-Specific DNA-Methyltransferase (Adenine-Specific) | 1998 |
Surface topography of phytochrome A deduced from specific chemical modification with iodoacetamide.
Topics: Amino Acid Sequence; Avena; Binding Sites; Cysteine; Disulfides; Iodoacetamide; Mass Spectrometry; Molecular Sequence Data; Peptide Fragments; Phytochrome; Phytochrome A; Plant Proteins; Protein Structure, Tertiary; Surface Properties; Trypsin | 1998 |
A conserved aspartate residue, Asp187, is important for Na+-dependent proline binding and transport by the Na+/proline transporter of Escherichia coli.
Topics: Amino Acid Sequence; Amino Acid Substitution; Amino Acid Transport Systems, Neutral; Aspartic Acid; Biological Transport, Active; Blotting, Western; Conserved Sequence; Cysteine; Iodoacetamide; Iodoacetic Acid; Kinetics; Ligands; Membrane Transport Proteins; Molecular Sequence Data; Proline; Protein Binding; Sequence Homology, Amino Acid; Sodium | 1998 |
Binding of soluble myelin basic protein to various conformational forms of alpha2-macroglobulin.
Topics: alpha-Macroglobulins; Blood Proteins; Calcium Chloride; Chlorides; Cysteine; Edetic Acid; Endopeptidases; Humans; Iodoacetamide; Magnesium Chloride; Myelin Basic Protein; Oxidation-Reduction; Protein Binding; Protein Conformation; Protein Denaturation; Solubility; Zinc Compounds | 1998 |
Modification of cysteine residues by alkylation. A tool in peptide mapping and protein identification.
Topics: Acrylamide; Alkylating Agents; Alkylation; Cysteine; Iodoacetamide; Peptide Mapping; Proteins; Pyridines | 1998 |
Sample preparation for peptide mapping--A pharmaceutical quality-control perspective.
Topics: Alkylation; Aziridines; Chemistry, Pharmaceutical; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Indicators and Reagents; Interferons; Iodoacetamide; Iodoacetic Acid; Oxidation-Reduction; Peptide Mapping; Peptides; Phosphines; Protein Denaturation; Pyridines; Quality Control; Solubility; Solvents; Trypsin | 1999 |
The catalytic mechanism of the glutathione-dependent dehydroascorbate reductase activity of thioltransferase (glutaredoxin).
Topics: Animals; Ascorbic Acid; Catalysis; Cysteine; Dehydroascorbic Acid; Enzyme Activation; Enzyme Inhibitors; Glutaredoxins; Glutathione; Iodoacetamide; Isoelectric Focusing; Kinetics; Liver; Models, Chemical; Oxidation-Reduction; Oxidoreductases; Protein Disulfide Reductase (Glutathione); Recombinant Proteins; Serine; Swine | 1999 |
The influenza virus M2 ion channel protein: probing the structure of the transmembrane domain in intact cells by using engineered disulfide cross-linking.
Topics: Amino Acid Sequence; Amino Acid Substitution; Binding Sites; Cross-Linking Reagents; Cysteine; Disulfides; HeLa Cells; Humans; Hydrogen-Ion Concentration; Influenza A virus; Iodoacetamide; Ion Channels; Kinetics; Molecular Sequence Data; Phenanthrolines; Sulfhydryl Reagents; Viral Matrix Proteins | 1999 |
Identification of the two zinc-bound cysteines in the ferric uptake regulation protein from Escherichia coli: chemical modification and mass spectrometry analysis.
Topics: Alkylation; Amino Acid Sequence; Bacterial Proteins; Binding Sites; Cysteine; Edetic Acid; Escherichia coli; Ferric Compounds; Hydrolysis; Iodoacetamide; Mass Spectrometry; Metalloproteins; Molecular Sequence Data; Repressor Proteins; Serine Endopeptidases; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time Factors; Zinc | 1999 |
Aminoethylcysteine can replace the function of the essential active site lysine of Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl coenzyme A reductase.
Topics: Alanine; Amino Acid Substitution; Binding Sites; Catalysis; Cysteine; Enzyme Activation; Hydroxymethylglutaryl CoA Reductases; Iodoacetamide; Lysine; Models, Molecular; Mutagenesis, Site-Directed; Pseudomonas | 1999 |
Effect of cysteine residues on the activity of arginyl-tRNA synthetase from Escherichia coli.
Topics: Acylation; Arginine-tRNA Ligase; Cysteine; Dithionitrobenzoic Acid; Enzyme Activation; Escherichia coli; Ethylmaleimide; Gene Expression Regulation, Enzymologic; Iodoacetamide; Kinetics; Mutagenesis, Site-Directed; Recombinant Proteins; Sulfhydryl Reagents; Titrimetry | 1999 |
Reactivity of the two essential cysteine residues of the periplasmic mercuric ion-binding protein, MerP.
Topics: Bacterial Proteins; Base Sequence; Carrier Proteins; Cysteine; DNA Primers; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Mercury; Oxidation-Reduction; Protein Binding; Proteins | 1999 |
Enzyme-catalyzed acylation of homoserine: mechanistic characterization of the Escherichia coli metA-encoded homoserine transsuccinylase.
Topics: Acylation; Acyltransferases; Binding Sites; Catalysis; Cloning, Molecular; Cysteine; Deuterium Oxide; Enzyme Activation; Enzyme Inhibitors; Escherichia coli; Escherichia coli Proteins; Homoserine; Homoserine O-Succinyltransferase; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Methionine; Solvents | 1999 |
Involvement of cysteine residues and domain interactions in the reversible unfolding of lipoxygenase-1.
Topics: Calorimetry; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Glycine max; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Lipoxygenase; Protein Conformation; Protein Denaturation; Protein Folding; Thermodynamics; Urea | 1999 |
Reactivity of the cysteine residues in the protein splicing active center of the Mycobacterium tuberculosis RecA intein.
Topics: Amino Acid Substitution; Bacterial Proteins; Binding Sites; Cysteine; Dithiothreitol; Fungal Proteins; Hydrogen-Ion Concentration; Hydroxylamine; Iodoacetamide; Mycobacterium tuberculosis; Protein Splicing; Rec A Recombinases; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Structure-Activity Relationship; Sulfhydryl Compounds; Sulfhydryl Reagents | 2000 |
Structure-function relationships in the carboxylic-ester-hydrolase superfamily. Disulfide bridge arrangement in porcine intestinal glycerol-ester hydrolase.
Topics: Animals; Binding Sites; Carboxylic Ester Hydrolases; Cholinesterases; Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Enzyme Stability; Intestinal Mucosa; Iodoacetamide; Lipase; Peptide Fragments; Peptide Mapping; Protein Binding; Protein Conformation; Sequence Analysis; Structure-Activity Relationship; Substrate Specificity; Swine | 2000 |
Identification of an artifact in the mass spectrometry of proteins derivatized with iodoacetamide.
Topics: Aged; Artifacts; Crystallins; Cysteine; Humans; Indicators and Reagents; Iodoacetamide; Mass Spectrometry; Methionine; Molecular Weight | 2000 |
Evidence for catalytic cysteine-histidine dyad in chalcone synthase.
Topics: Acyltransferases; Amino Acid Substitution; Binding Sites; Catalysis; Cysteine; Diethyl Pyrocarbonate; Enzyme Inhibitors; Escherichia coli; Flavanones; Flavonoids; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Malonyl Coenzyme A; Mutagenesis, Site-Directed; Plants; Recombinant Fusion Proteins; Resveratrol; Stilbenes | 2000 |
Mechanism of chalcone synthase. pKa of the catalytic cysteine and the role of the conserved histidine in a plant polyketide synthase.
Topics: Acyltransferases; Alkylating Agents; Anions; Binding Sites; Carboxy-Lyases; Catalysis; Conserved Sequence; Cysteine; Dose-Response Relationship, Drug; Enzyme Inhibitors; Glutamine; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Models, Chemical; Models, Molecular; Protein Binding; Time Factors | 2000 |
Determination of the pKa value of C115 in MurA (UDP-N-acetylglucosamine enolpyruvyltransferase) from Enterobacter cloacae.
Topics: Alkyl and Aryl Transferases; Alkylating Agents; Catalysis; Cysteine; DNA Mutational Analysis; Enterobacter cloacae; Enzyme Activation; Enzyme Inhibitors; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Mutagenesis, Site-Directed; Peptide Fragments; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Substrate Specificity; Trypsin | 2000 |
The role of cysteine residues in tellurite resistance mediated by the TehAB determinant.
Topics: Alanine; Alkylating Agents; Bacterial Proteins; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Drug Resistance; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Iodoacetamide; Iodoacetates; Ligands; Microbial Sensitivity Tests; Mutagenesis, Site-Directed; Phenotype; Plasmids; Protein Binding; Protein Conformation; Protein Denaturation; S-Adenosylmethionine; Sulfhydryl Reagents; Tellurium; Up-Regulation | 2000 |
Biological activity of soluble CD100. I. The extracellular region of CD100 is released from the surface of T lymphocytes by regulated proteolysis.
Topics: 3T3 Cells; Adjuvants, Immunologic; Animals; Antigens, CD; Cell Membrane; COS Cells; Cysteine; Dimerization; Edetic Acid; Egtazic Acid; Endopeptidases; Enzyme Inhibitors; Extracellular Space; Humans; Hydrolysis; Iodoacetamide; Jurkat Cells; Membrane Glycoproteins; Metalloendopeptidases; Mice; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Semaphorins; Solubility; Staurosporine; T-Lymphocytes; Transfection | 2001 |
Enhanced potency of human Sonic hedgehog by hydrophobic modification.
Topics: Acyl Coenzyme A; Amides; Amino Acid Substitution; Animals; Cell Line; Circular Dichroism; Cysteine; Ethylmaleimide; Fatty Acids; Formaldehyde; Hedgehog Proteins; Humans; Indicators and Reagents; Intracellular Signaling Peptides and Proteins; Iodoacetamide; Membrane Proteins; Mice; Mice, Inbred C3H; Patched Receptors; Peptide Fragments; Protein Binding; Protein Processing, Post-Translational; Proteins; Receptors, Cell Surface; Signal Transduction; Spectrometry, Mass, Electrospray Ionization; Sulfhydryl Compounds; Thiazoles; Thiazolidines; Trans-Activators; Up-Regulation | 2001 |
Disulfide bond structure of the AVR9 elicitor of the fungal tomato pathogen Cladosporium fulvum: evidence for a cystine knot.
Topics: Alkylation; Amino Acid Sequence; Carboxypeptidases; Carboxypeptidases A; Cladosporium; Cysteine; Cystine; Disulfides; Ethylmaleimide; Fungal Proteins; Iodoacetamide; Molecular Sequence Data; Necrosis; Phosphines; Plant Diseases; Plant Leaves; Reducing Agents; Solanum lycopersicum; Sulfhydryl Compounds; Virulence | 2001 |
Alkylation kinetics of proteins in preparation for two-dimensional maps: a matrix assisted laser desorption/ionization-mass spectrometry investigation.
Topics: Alkylation; Animals; Binding Sites; Cattle; Chickens; Cholic Acids; Cysteine; Electrophoresis, Gel, Two-Dimensional; Iodoacetamide; Kinetics; Lactalbumin; Lysine; Muramidase; Peptide Mapping; Proteins; Sodium Dodecyl Sulfate; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2001 |
Mechanism of integrin activation by disulfide bond reduction.
Topics: Alkylating Agents; Amino Acid Sequence; Cysteine; Disulfides; Dithiothreitol; Humans; Hydrolysis; Iodoacetamide; Ligands; Molecular Sequence Data; Oxidation-Reduction; Peptide Mapping; Platelet Glycoprotein GPIIb-IIIa Complex; Protein Binding; Protein Conformation; Reducing Agents; Serine Endopeptidases; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2001 |
Overalkylation of a protein digest with iodoacetamide.
Topics: Actins; Alkylation; Animals; Cattle; Chromatography, Liquid; Cysteine; Hydrolysis; Iodoacetamide; Iodoacetic Acid; Mass Spectrometry; Oxidation-Reduction; Peptides; Proteins; Sulfhydryl Reagents | 2001 |
Extracellular cysteines define ectopeptidase (APN, CD13) expression and function.
Topics: Amidohydrolases; Antigens, Surface; CD13 Antigens; Cysteine; DNA Primers; Ethylmaleimide; Green Fluorescent Proteins; Hexosaminidases; Humans; Immunoblotting; Iodoacetamide; Luminescent Proteins; Microscopy, Fluorescence; Mutagenesis, Site-Directed; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; Polymerase Chain Reaction; Protein Disulfide-Isomerases; Recombinant Fusion Proteins; Subcellular Fractions; U937 Cells | 2002 |
The local electrostatic environment determines cysteine reactivity of tubulin.
Topics: Acetamides; Animals; Cysteine; Electrons; Enzyme Inhibitors; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Ligands; Lysine; Mass Spectrometry; Models, Molecular; Palmitic Acids; Peptides; Protein Binding; Rats; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Static Electricity; Sulfides; Time Factors; Trypsin; Tubulin | 2002 |
Thiol-induced discharge of acontial nematocytes.
Topics: Alkylating Agents; Animals; Anthozoa; Bites and Stings; Chemoreceptor Cells; Cysteine; Disulfides; Glutathione; Homocysteine; Iodoacetamide; Oxidation-Reduction; Penicillamine; Sulfhydryl Compounds | 2002 |
The roles of thiols in the bacterial organomercurial lyase (MerB).
Topics: Amino Acid Sequence; Bacterial Proteins; Cysteine; Cytosol; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Glutathione; Hydroxymercuribenzoates; Iodoacetamide; Kinetics; Lyases; Molecular Sequence Data; Mutagenesis, Site-Directed; Organomercury Compounds; Sequence Homology, Amino Acid; Substrate Specificity; Sulfhydryl Compounds | 2002 |
Mechanism of the beta-ketoacyl synthase reaction catalyzed by the animal fatty acid synthase.
Topics: 3-Oxoacyl-(Acyl-Carrier-Protein) Synthase; Acyl Coenzyme A; Animals; Bicarbonates; Binding Sites; Catalysis; Chickens; Cysteine; Decarboxylation; Enzyme Inhibitors; Fatty Acid Synthases; Humans; Iodoacetamide; Malonyl Coenzyme A; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Rats; Substrate Specificity | 2002 |
Function and solution structure of huwentoxin-IV, a potent neuronal tetrodotoxin (TTX)-sensitive sodium channel antagonist from Chinese bird spider Selenocosmia huwena.
Topics: Amino Acid Sequence; Animals; Binding Sites; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dose-Response Relationship, Drug; Electrophysiology; Female; Ganglia, Spinal; Inhibitory Concentration 50; Iodoacetamide; Magnetic Resonance Spectroscopy; Male; Models, Molecular; Molecular Sequence Data; Neurons; Neurotoxins; Protein Binding; Protein Structure, Secondary; Rats; Rats, Sprague-Dawley; Sequence Homology, Amino Acid; Sodium; Sodium Channels; Software; Spider Venoms; Spiders; Tetrodotoxin; Time Factors | 2002 |
Familial amyotrophic lateral sclerosis mutants of copper/zinc superoxide dismutase are susceptible to disulfide reduction.
Topics: Amino Acid Substitution; Animals; Binding Sites; Cysteine; Disulfides; Dithiothreitol; Humans; Iodoacetamide; Kinetics; Mice; Motor Neuron Disease; Mutagenesis, Site-Directed; Mutation; Organ Specificity; Oxidation-Reduction; Protein Structure, Secondary; Recombinant Proteins; Superoxide Dismutase | 2003 |
Chemical modifications of proteins as an aid to sequence analysis.
Topics: Acrylamide; Alkylation; Cysteine; Fluoresceins; Formates; Iodoacetamide; Oxidation-Reduction; Phosphoserine; Proteins; Sequence Analysis, Protein | 2003 |
Biochemical characterization of yeast mitochondrial Grx5 monothiol glutaredoxin.
Topics: Carbonic Anhydrases; Catalysis; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Electrophysiology; Enzyme Inhibitors; Escherichia coli; Glutaredoxins; Glutathione; Glutathione Disulfide; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Mitochondria; Models, Biological; Mutation; Oxidation-Reduction; Oxidoreductases; Oxygen; Plasmids; Protein Biosynthesis; Proteins; Saccharomyces cerevisiae; Time Factors | 2003 |
The effect of oxidation or alkylation on the separation of wool keratin proteins by two-dimensional gel electrophoresis.
Topics: Alkylating Agents; Alkylation; Amino Acids; Animals; Cysteine; Electrophoresis, Gel, Two-Dimensional; Hydrogen Peroxide; Iodoacetamide; Isoelectric Point; Keratins; Kinetics; Mass Spectrometry; Molecular Weight; Oxidants; Oxidation-Reduction; Sheep; Wool | 2003 |
Thiol-reactive dyes for fluorescence labeling of proteomic samples.
Topics: Animals; Cysteine; Fluorescent Dyes; Humans; Iodoacetamide; Isoelectric Point; Maleimides; Proteins; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds | 2003 |
On-chip protein sample desalting and preparation for direct coupling with electrospray ionization mass spectrometry.
Topics: Adsorption; Cysteine; Cytochromes c; Dithiothreitol; Indicators and Reagents; Insulin; Iodoacetamide; Lactoglobulins; Propranolol; Protein Array Analysis; Proteins; Salts; Spectrometry, Mass, Electrospray Ionization; Urea | 2003 |
[Relations between digitalis and sulfhydryl compounds: experimental comparative electrocardiographic and morphological study between digitalis and iodoacetamide; protective effects of cysteine in digitalis poisoning].
Topics: Cardiovascular Agents; Cysteine; Digitalis; Electrocardiography; Humans; Iodoacetamide; Iodoacetates; Sulfhydryl Compounds | 1957 |
Chemical modification of catalytically essential functional groups of NAD-dependent hydrogenase from Ralstonia eutropha H16.
Topics: Cupriavidus necator; Cysteine; Diethyl Pyrocarbonate; Histidine; Hydrogen-Ion Concentration; Hydrogenase; Iodoacetamide; Kinetics; NAD | 2003 |
N-t-butyliodoacetamide and iodoacetanilide: two new cysteine alkylating reagents for relative quantitation of proteins.
Topics: Acetanilides; Alkylating Agents; Alkylation; Cysteine; Indicators and Reagents; Iodoacetamide; Lactoperoxidase; Peptide Mapping; Proteins; Proteomics; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2004 |
Isotope-coded affinity tag approach to identify and quantify oxidant-sensitive protein thiols.
Topics: Affinity Labels; Animals; Creatine Kinase; Cysteine; Hydrogen Peroxide; Iodoacetamide; Isotope Labeling; Mass Spectrometry; Muscle, Skeletal; Oxidants; Proteome; Rabbits; Sulfhydryl Compounds | 2004 |
Direct observation of covalent adducts with Cys34 of human serum albumin using mass spectrometry.
Topics: Cysteine; Dithiothreitol; Glutathione; Gold Compounds; Humans; Iodoacetamide; Serum Albumin; Spectrometry, Mass, Electrospray Ionization | 2004 |
About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis.
Topics: Alkylation; Animals; Cattle; Cysteine; Cystine; Disulfides; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Gels; Hydrogen Peroxide; Hydrogen-Ion Concentration; Iodoacetamide; Maleimides; Mass Spectrometry; Mitochondria; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds; Temperature; Time Factors; Water | 2004 |
Probing Dictyostelium severin structure and function by cross linking to actin.
Topics: Actins; Amino Acid Sequence; Animals; Cysteine; Dictyostelium; Gelsolin; Hydrogen-Ion Concentration; Iodoacetamide; Microfilament Proteins; Models, Structural; Molecular Sequence Data; Mutagenesis; Peptide Fragments; Protein Binding; Protozoan Proteins; Sequence Homology | 2004 |
Probing the mechanism of hamster arylamine N-acetyltransferase 2 acetylation by active site modification, site-directed mutagenesis, and pre-steady state and steady state kinetic studies.
Topics: Acetamides; Acetyl Coenzyme A; Acetylation; Alkylating Agents; Amino Acid Sequence; Amino Acid Substitution; Animals; Arylamine N-Acetyltransferase; Binding Sites; Cricetinae; Cysteine; Deuterium; Enzyme Inhibitors; Hydrogen-Ion Concentration; Iodoacetamide; Isoenzymes; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nitrophenols; Papain; Protein Conformation; Recombinant Proteins | 2004 |
Mapping of factor XIII solvent accessibility as a function of activation state using chemical modification methods.
Topics: Acetic Anhydrides; Acetylation; Alkylation; Amino Acid Sequence; Cysteine; Ethylmaleimide; Factor XIII; Factor XIIIa; Humans; Hydrolysis; Intercellular Signaling Peptides and Proteins; Iodoacetamide; Lysine; Molecular Sequence Data; Peptide Mapping; Peptides; Solutions; Solvents; Trypsin | 2004 |
Targeted mutagenesis of the Mycobacterium smegmatis mca gene, encoding a mycothiol-dependent detoxification protein.
Topics: Amidohydrolases; Anti-Bacterial Agents; Bridged Bicyclo Compounds; Cysteine; Dinitrochlorobenzene; Disaccharides; Enzyme Inhibitors; Ethylmaleimide; Gene Deletion; Genes, Bacterial; Genetic Complementation Test; Glycopeptides; Inositol; Iodoacetamide; Mutagenesis, Insertional; Mycobacterium smegmatis; Mycobacterium tuberculosis; Naphthoquinones; Oxidants; Pyrazoles; Rifamycins; Streptomycin; Sulfhydryl Compounds; Vitamin K 3 | 2004 |
Identification of thioredoxin h-reducible disulphides in proteomes by differential labelling of cysteines: insight into recognition and regulation of proteins in barley seeds by thioredoxin h.
Topics: Allergens; Amino Acid Sequence; Antigens, Plant; Cysteine; Disulfides; Electrophoresis, Gel, Two-Dimensional; Hordeum; Iodoacetamide; Molecular Sequence Data; Oxidation-Reduction; Peptide Mapping; Plant Proteins; Proteome; Pyridines; Seeds; Sequence Homology, Amino Acid; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining and Labeling; Sulfhydryl Compounds; Thioredoxin h; Thioredoxins; Trypsin Inhibitor, Kunitz Soybean | 2005 |
Dual-targeted labeling of proteins using cysteine and selenomethionine residues.
Topics: Bacterial Proteins; Calmodulin; Cysteine; Energy Transfer; Escherichia coli; Fluorescence; Fluorescent Dyes; Hemolysin Proteins; Iodoacetamide; Maleimides; Mutagenesis, Site-Directed; Naphthalenesulfonates; Proteins; Selenomethionine | 2005 |
Development of a direct and sensitive detection method for DNA-binding proteins based on electrophoretic mobility shift assay and iodoacetamide derivative labeling.
Topics: Cysteine; DNA-Binding Proteins; Electrophoresis, Polyacrylamide Gel; Electrophoretic Mobility Shift Assay; Iodoacetamide; Sensitivity and Specificity | 2005 |
Modifying specific cysteines of the electrophile-sensing human Keap1 protein is insufficient to disrupt binding to the Nrf2 domain Neh2.
Topics: Animals; Calorimetry; Chalcone; Chalcones; Cloning, Molecular; Cysteine; Dinitrobenzenes; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; Enzyme Induction; Humans; Immunologic Factors; Intracellular Signaling Peptides and Proteins; Iodoacetamide; Isothiocyanates; Kelch-Like ECH-Associated Protein 1; Models, Molecular; Multiprotein Complexes; NF-E2-Related Factor 2; Prostaglandin D2; Protein Binding; Protein Structure, Tertiary; Proteins; Sulfoxides; Thiocyanates; Trans-Activators; Vitamin K 3 | 2005 |
A simplified 2-D electrophoresis protocol with the aid of an organic disulfide.
Topics: Animals; Cysteine; Disulfides; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Hydrogen-Ion Concentration; Image Processing, Computer-Assisted; Iodoacetamide; Liver; Mass Spectrometry; Mercaptoethanol; Mice; Organic Chemicals; Peptides; Silver Staining; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2005 |
Kinetic comparison of procaspase-3 and caspase-3.
Topics: Binding Sites; Caspase 3; Caspases; Catalysis; Crystallography, X-Ray; Cysteine; Diethyl Pyrocarbonate; Enzyme Activation; Enzyme Precursors; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Mutation | 2005 |
Metallothionein disulfides are present in metallothionein-overexpressing transgenic mouse heart and increase under conditions of oxidative stress.
Topics: Animals; Antineoplastic Agents; Blotting, Western; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Doxorubicin; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Iodoacetamide; Mass Spectrometry; Metallothionein; Mice; Mice, Transgenic; Myocardium; Oxidative Stress; Peptides; Protein Structure, Tertiary; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time Factors; Trypsin; Zinc | 2006 |
Mutation of F417 but not of L418 or L420 in the lipid binding domain decreases the activity of triacylglycerol hydrolase.
Topics: Acylation; Amino Acid Sequence; Animals; Binding Sites; Butyrates; Catalysis; Cell Line; Chlorocebus aethiops; COS Cells; Cysteine; Gene Deletion; Gene Expression; Humans; Hymecromone; Iodoacetamide; Lipase; Mercaptoethanol; Mutagenesis, Site-Directed; Mutation; Nitrophenols; Phenylalanine; Point Mutation; Protein Folding; Recombinant Proteins; Sequence Homology, Amino Acid; Spodoptera; Substrate Specificity; Transfection | 2006 |
Mechanistic characterization of the MSDH (methylmalonate semialdehyde dehydrogenase) from Bacillus subtilis.
Topics: 2,2'-Dipyridyl; Apoenzymes; Bacillus subtilis; Bicarbonates; Catalysis; Cysteine; Decarboxylation; Disulfides; Holoenzymes; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Methylmalonate-Semialdehyde Dehydrogenase (Acylating); Mutation; NAD; Oxidation-Reduction; Protein Binding; Time Factors | 2006 |
Variable reactivity of an engineered cysteine at position 338 in cystic fibrosis transmembrane conductance regulator reflects different chemical states of the thiol.
Topics: Alkylating Agents; Animals; Catalysis; Copper; Cysteine; Cystic Fibrosis Transmembrane Conductance Regulator; Disulfides; Dithiothreitol; Glutathione; Humans; Hydrogen Peroxide; Hydrogen-Ion Concentration; Iodoacetamide; Mercaptoethanol; Mesylates; Metals; Models, Biological; Oocytes; Oxygen; Phenotype; Protein Engineering; Sulfhydryl Compounds; Sulfinic Acids; Sulfonic Acids; Time Factors; Xenopus | 2006 |
Substrate-assisted cysteine deprotonation in the mechanism of dimethylargininase (DDAH) from Pseudomonas aeruginosa.
Topics: Amidohydrolases; Binding Sites; Citrulline; Cobalt; Cysteine; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Lysine; Mass Spectrometry; Models, Chemical; omega-N-Methylarginine; Protons; Pseudomonas aeruginosa; Spectrophotometry, Ultraviolet; Spectrum Analysis; Thiourea; X-Rays; Zinc | 2006 |
Identification of catalytic cysteine, histidine, and lysine residues in Escherichia coli homoserine transsuccinylase.
Topics: Catalysis; Cysteine; Escherichia coli; Histidine; Homoserine O-Succinyltransferase; Hydrogen-Ion Concentration; Iodoacetamide; Lysine; Models, Molecular; Mutagenesis, Site-Directed | 2007 |
Location and role of free cysteinyl residues in the Sindbis virus E1 and E2 glycoproteins.
Topics: Amino Acid Sequence; Animals; Capsid; Cell Line; Chromatography, Liquid; Cricetinae; Cysteine; Disulfides; Iodoacetamide; Mass Spectrometry; Membrane Glycoproteins; Molecular Sequence Data; Mutation; Protein Conformation; Viral Envelope Proteins | 2007 |
Protein targets of reactive electrophiles in human liver microsomes.
Topics: Acetaminophen; Alkylating Agents; Biotin; Blotting, Western; Calreticulin; Cell Line; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Cysteine; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Glutathione Transferase; Humans; Iodoacetamide; Microsomes, Liver; Molecular Structure; Protein Binding; Proteins; Tandem Mass Spectrometry | 2007 |
Reactivity recognition by TRPA1 channels.
Topics: Calcium Channels; Cysteine; Humans; Iodoacetamide; Isothiocyanates; Nerve Tissue Proteins; Patch-Clamp Techniques; Transient Receptor Potential Channels; TRPA1 Cation Channel | 2007 |
Time-dependent inactivation of P450 3A4 by raloxifene: identification of Cys239 as the site of apoprotein alkylation.
Topics: Alkylating Agents; Alkylation; Apoproteins; Binding Sites; Cysteine; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System; Endopeptidase K; Enzyme Inhibitors; Estrogen Antagonists; Glutathione; Iodoacetamide; Kinetics; Midazolam; Molecular Structure; Quinolines; Raloxifene Hydrochloride; Recombinant Proteins; Sequence Analysis, Protein; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Ultraviolet; Time Factors | 2007 |
Copper sensing based on the far-red fluorescent protein, HcRed, from Heteractis crispa.
Topics: Animals; Biosensing Techniques; Cations, Divalent; Circular Dichroism; Copper; Cysteine; Diethyl Pyrocarbonate; Histidine; Iodoacetamide; Luminescent Proteins; Sea Anemones; Sensitivity and Specificity; Spectrometry, Fluorescence | 2007 |
Perturbations in factor XIII resulting from activation and inhibition examined by solution based methods and detected by MALDI-TOF MS.
Topics: Acetylation; Alkylation; Calcium; Catalysis; Cations, Divalent; Computer Simulation; Cysteine; Deuterium Exchange Measurement; Enzyme Activation; Enzyme Inhibitors; Factor XIII; Factor XIIIa; Glutamine; Humans; Iodoacetamide; Lysine; Models, Molecular; Peptides; Recombinant Fusion Proteins; Solutions; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thrombin | 2007 |
Identification of the highly reactive cysteine 151 in the chemopreventive agent-sensor Keap1 protein is method-dependent.
Topics: Antioxidants; Biotinylation; Chromatography, High Pressure Liquid; Cysteine; Intracellular Signaling Peptides and Proteins; Iodoacetamide; Kelch-Like ECH-Associated Protein 1; NF-E2-Related Factor 2; Peptides; Protective Agents; Proteomics; Response Elements; Tandem Mass Spectrometry | 2007 |
A biosensor of S100A4 metastasis factor activation: inhibitor screening and cellular activation dynamics.
Topics: Animals; Biosensing Techniques; Calcium; Cell Movement; Cell-Free System; Circular Dichroism; Cysteine; Egtazic Acid; Fibroblasts; Fluorescence Polarization; Humans; Iodoacetamide; Lysophospholipids; Mice; Models, Molecular; Molecular Structure; Nonmuscle Myosin Type IIA; Phenothiazines; Protein Binding; Protein Conformation; Pyrimidinones; Recombinant Proteins; S100 Calcium-Binding Protein A4; S100 Proteins; Spectrometry, Fluorescence; Trifluoperazine | 2008 |
Isotope-coded, iodoacetamide-based reagent to determine individual cysteine pK(a) values by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Topics: Acetanilides; Cysteine; Deuterium; Hydrogen-Ion Concentration; Indicators and Reagents; Iodoacetamide; Isotopes; Kinetics; Oxidation-Reduction; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry; Thioredoxins; Titrimetry | 2008 |
High glucose oxidizes SERCA cysteine-674 and prevents inhibition by nitric oxide of smooth muscle cell migration.
Topics: Animals; Biotinylation; Calcineurin; Calcium; Calmodulin; Cell Movement; Cyclic N-Oxides; Cysteine; Glucose; Glutathione; Humans; Iodoacetamide; Mannose; Myocytes, Smooth Muscle; Nitric Oxide; Nitric Oxide Donors; Nitric Oxide Synthase Type II; Oxidation-Reduction; Rats; S-Nitroso-N-Acetylpenicillamine; Sarcoplasmic Reticulum Calcium-Transporting ATPases; Spin Labels; Sulfonic Acids; Superoxide Dismutase | 2008 |
Synthesis, characterization, and application of iodoacetamide derivatives utilized for the ALiPHAT strategy.
Topics: Alkylation; Amino Acid Sequence; Cysteine; Factor VIIIa; Hydrophobic and Hydrophilic Interactions; Iodoacetamide; Molecular Sequence Data; Peptides; Spectrometry, Mass, Electrospray Ionization | 2008 |
Steady-state and pre-steady-state kinetic evaluation of severe acute respiratory syndrome coronavirus (SARS-CoV) 3CLpro cysteine protease: development of an ion-pair model for catalysis.
Topics: Acrylates; Alkylation; Catalysis; Coronavirus 3C Proteases; Cysteine; Cysteine Endopeptidases; Dipeptides; Enzyme Activation; Enzyme Inhibitors; Enzyme Stability; Histidine; Hydrogen-Ion Concentration; Iodoacetamide; Ions; Kinetics; Models, Biological; Severe acute respiratory syndrome-related coronavirus; Solvents; Static Electricity; Transferases; Viral Proteins | 2008 |
Probing conformational changes of prestin with thiol-reactive optical switches.
Topics: Animals; Anion Transport Proteins; Biotin; Cell Membrane; Cysteine; Electric Capacitance; Guinea Pigs; Hair Cells, Auditory, Outer; Humans; Iodoacetamide; Maleimides; Optics and Photonics; Protein Conformation; Sulfhydryl Compounds; Transfection | 2008 |
Cysteine pK(a) values for the bacterial peroxiredoxin AhpC.
Topics: Absorption; Alkylation; Amino Acid Sequence; Bacterial Proteins; Binding, Competitive; Buffers; Catalytic Domain; Crystallography, X-Ray; Cysteine; Horseradish Peroxidase; Hydrogen Peroxide; Hydrogen-Ion Concentration; Incubators; Iodoacetamide; Molecular Sequence Data; Peroxiredoxins; Protein Denaturation; Protein Stability; Salmonella typhimurium; Time Factors | 2008 |
New iodo-acetamido cyanines for labeling cysteine thiol residues. A strategy for evaluating plasma proteins and their oxido-redox status.
Topics: Blood Proteins; Carbocyanines; Cysteine; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Glomerulosclerosis, Focal Segmental; Humans; Iodoacetamide; Oxidation-Reduction; Proteomics; Reproducibility of Results; Sensitivity and Specificity; Sulfhydryl Compounds | 2009 |
Enhanced electron transfer dissociation through fixed charge derivatization of cysteines.
Topics: Alkylation; Amino Acid Sequence; Cysteine; Electron Transport; Ethylamines; Iodoacetamide; Oxidation-Reduction; Peptides; Proteomics; Trimethyl Ammonium Compounds | 2009 |
A new method for the detection and characterization of alpha-lipoic acid mixed disulphides.
Topics: Calcium-Calmodulin-Dependent Protein Kinase Type 2; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Glutathione; Glutathione Disulfide; Iodoacetamide; Peptide Fragments; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds; Tandem Mass Spectrometry; Thioctic Acid | 2010 |
Soybean (Glycine max) urease: significance of sulfhydryl groups in urea catalysis.
Topics: Biocatalysis; Cysteine; Dithionitrobenzoic Acid; Enzyme Assays; Ethylmaleimide; Glycine max; Iodoacetamide; Kinetics; p-Chloromercuribenzoic Acid; Plant Proteins; Sulfhydryl Compounds; Sulfhydryl Reagents; Time Factors; Urea; Urease | 2010 |
Collagenolytic activity in sonic extracts of Tannerella forsythia.
Topics: Bacteroides; Calcium Chloride; Cathepsins; Cell Line, Tumor; Chelating Agents; Collagen Type I; Cysteine; Cysteine Proteinase Inhibitors; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Enzyme Inhibitors; Enzyme Precursors; Ethylmaleimide; Gelatinases; Humans; Iodoacetamide; Iodoacetic Acid; Leucine; Leupeptins; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; Phenylmethylsulfonyl Fluoride; Protease Inhibitors; Serine Proteinase Inhibitors; Tosyllysine Chloromethyl Ketone; Tosylphenylalanyl Chloromethyl Ketone | 2010 |
Cysteine 351 is an essential nucleophile in catalysis by Porphyromonas gingivalis peptidylarginine deiminase.
Topics: Catalysis; Cysteine; Hydrogen-Ion Concentration; Hydrolases; Iodoacetamide; Iodoacetic Acid; Ligands; Mass Spectrometry; Mutagenesis, Site-Directed; Peptide Mapping; Porphyromonas gingivalis; Protein Binding; Protein-Arginine Deiminases; Recombinant Proteins | 2010 |
Hypersensitive response to over-reactive cysteines.
Topics: Chromatography, High Pressure Liquid; Cysteine; Iodoacetamide; Isotope Labeling; Mass Spectrometry; Proteins; Proteome | 2011 |
In-depth analysis of cysteine oxidation by the RBC proteome: advantage of peroxiredoxin II knockout mice.
Topics: Amino Acid Sequence; Animals; Cysteine; Cytoskeletal Proteins; Erythrocytes; Gene Knockout Techniques; Homeostasis; Iodoacetamide; Mass Spectrometry; Mice; Mice, Inbred C57BL; Mice, Knockout; Molecular Sequence Data; Oxidation-Reduction; Peptide Fragments; Peroxiredoxins; Protein Interaction Maps; Proteome | 2012 |
Implication of cysteine residues in the selection of oxorhenium inhibitors of cyclophilin hCyp18.
Topics: Chromatography, Liquid; Combinatorial Chemistry Techniques; Cyclophilins; Cyclosporine; Cysteine; Enzyme Inhibitors; Iodoacetamide; Kinetics; Methylation; Models, Molecular; Oxidation-Reduction; Rhenium; Spectrometry, Mass, Electrospray Ionization | 2012 |
Proteomic analysis of redox-dependent changes using cysteine-labeling 2D DIGE.
Topics: Cysteine; Humans; Immunoblotting; Iodoacetamide; Oxidation-Reduction; Proteomics; Reproducibility of Results; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining and Labeling; Tissue Culture Techniques; Two-Dimensional Difference Gel Electrophoresis | 2012 |
Identification of consistent alkylation of cysteine-less peptides in a proteomics experiment.
Topics: Alkylating Agents; Alkylation; Amino Acid Sequence; Chromatography, Liquid; Cysteine; Databases, Protein; Iodoacetamide; Molecular Sequence Data; Oligopeptides; Proteomics; Tandem Mass Spectrometry | 2012 |
Improving the stability of the EC1 domain of E-cadherin by thiol alkylation of the cysteine residue.
Topics: Alkylation; Cadherins; Circular Dichroism; Cysteine; Iodoacetamide; Iodoacetates; Maleimides; Polyethylene Glycols; Protein Folding; Protein Structure, Tertiary; Sulfides; Temperature | 2012 |
Regulation of Ca(2+)/calmodulin-dependent protein kinase phosphatase (CaMKP) by oxidation/reduction at Cys-359.
Topics: Amino Acid Sequence; Animals; Calcium Signaling; Cysteine; Disulfides; Dose-Response Relationship, Drug; Down-Regulation; Enzyme Activation; HEK293 Cells; Humans; Hydrogen Peroxide; Iodoacetamide; Mice; Models, Molecular; Molecular Sequence Data; Oxidation-Reduction; Oxidative Stress; Phosphoprotein Phosphatases; Point Mutation; Protein Structure, Tertiary; Rats; Time Factors | 2012 |
Mechanistic studies of the spore photoproduct lyase via a single cysteine mutation.
Topics: Bacillus subtilis; Catalytic Domain; Cysteine; Dithionite; Electron Transport; Iodoacetamide; Kinetics; Models, Molecular; Mutation; Proteins; Protons; Thymine | 2012 |
Peptide peak intensities enhanced by cysteine modifiers and MALDI TOF MS.
Topics: Alkylation; Amino Acid Sequence; Animals; Cattle; Cysteine; Iodoacetamide; Molecular Sequence Data; Peptide Fragments; Serum Albumin, Bovine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2012 |
An enzymatic route to selenazolines.
Topics: Amino Acid Sequence; Cysteine; Iodoacetamide; Multienzyme Complexes; Oxazoles; Peptides, Cyclic; Selenium; Selenocysteine; Serine; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thiazoles; Threonine | 2013 |
Analysis of mutants disrupted in bacillithiol metabolism in Staphylococcus aureus.
Topics: Bacterial Proteins; Biosynthetic Pathways; Chromatography, High Pressure Liquid; Cysteine; Glucosamine; Iodoacetamide; Metals; Microbial Viability; Mutation; Oxidants; Pyruvaldehyde; Staphylococcus aureus; Sulfhydryl Compounds; Time Factors | 2013 |
Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids.
Topics: Ascorbic Acid; Cyclohexanones; Cysteine; Dithiothreitol; Ethylmaleimide; Iodoacetamide; Methyl Methanesulfonate; Oxidation-Reduction; Proteins; Reactive Oxygen Species; Spectrometry, Mass, Electrospray Ionization; Sulfenic Acids; Sulfhydryl Compounds | 2013 |
Imidazolium-based iodoacetamide functional tags: design, synthesis, and property study for cysteinyl-peptide analysis by mass spectrometry.
Topics: Cysteine; Imidazoles; Imidazolines; Iodoacetamide; Mass Spectrometry; Peptides; Proteins | 2014 |
[Influence of overalkylation in enzymatic digestion on the qualitative and quantitative analysis of proteins].
Topics: Alkylating Agents; Alkylation; Amino Acids; Cysteine; Disulfides; Iodoacetamide; Peptides; Proteins | 2013 |
Profiling thiol metabolites and quantification of cellular glutathione using FT-ICR-MS spectrometry.
Topics: Cell Line, Tumor; Cysteine; Dipeptides; Glutathione; Glutathione Disulfide; Humans; Iodoacetamide; Isotope Labeling; Mass Spectrometry; Molecular Probes; Spectroscopy, Fourier Transform Infrared; Sulfhydryl Compounds | 2014 |
Inactivation of the Mycobacterium tuberculosis antigen 85 complex by covalent, allosteric inhibitors.
Topics: Acyltransferases; Allosteric Regulation; Anti-Inflammatory Agents, Non-Steroidal; Antigens, Bacterial; Azoles; Biocatalysis; Catalytic Domain; Chloromercuribenzoates; Crystallography, X-Ray; Cysteine; Enzyme Activation; Enzyme Inhibitors; Hydrogen Bonding; Iodoacetamide; Isoindoles; Models, Molecular; Molecular Structure; Mutation; Organoselenium Compounds; Protein Conformation; Protein Structure, Secondary | 2014 |
Infrared spectroscopy provides insight into the role of dioxygen in the nitrosylation pathway of a [2Fe2S] cluster iron-sulfur protein.
Topics: Cysteine; Ferredoxins; Hydrogen-Ion Concentration; Iodoacetamide; Iron; Iron-Sulfur Proteins; Nitric Oxide; Nitrogen; Nitroso Compounds; Oxygen; Plant Proteins; Spectrophotometry, Infrared; Spinacia oleracea | 2014 |
A new BODIPY-based long-wavelength fluorescent probe for chromatographic analysis of low-molecular-weight thiols.
Topics: Acetylcysteine; Animals; Boron Compounds; Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Cysteine; Dipeptides; Fluorescent Dyes; Glutathione; Homocysteine; Iodoacetamide; Limit of Detection; Male; Mice; Penicillamine; Sulfhydryl Compounds | 2014 |
A method for studies on interactions between a gold-based drug and plasma proteins based on capillary electrophoresis with inductively coupled plasma mass spectrometry detection.
Topics: Antirheumatic Agents; Auranofin; Cysteine; Electrophoresis, Capillary; Gold; Humans; Iodoacetamide; Iopanoic Acid; Kinetics; Limit of Detection; Serum Albumin; Spectrophotometry, Atomic; Staining and Labeling | 2015 |
The Cysteine-rich Domain of the DHHC3 Palmitoyltransferase Is Palmitoylated and Contains Tightly Bound Zinc.
Topics: Amino Acid Motifs; Animals; Biotin; Catalytic Domain; Chelating Agents; Cysteine; Iodoacetamide; Ions; Lipoylation; Lysine; Mass Spectrometry; Membrane Proteins; Metals; Mice; Mutation; Protein Binding; Protein Processing, Post-Translational; Sf9 Cells; Spodoptera; Zinc | 2015 |
Ion source-dependent performance of 4-vinylpyridine, iodoacetamide, and N-maleoyl derivatives for the detection of cysteine-containing peptides in complex proteomics.
Topics: Alkylation; Amino Acid Sequence; Amino Acids; Cysteine; Escherichia coli; Escherichia coli Proteins; Humans; Hydrolysis; Iodoacetamide; Maleates; Models, Molecular; Peptides; Proteomics; Pyridines; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2016 |
A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity.
Topics: Animals; Cysteine; Iodoacetamide; Mass Spectrometry; Protein Processing, Post-Translational; Proteome | 2017 |
Modification of Cysteine.
Topics: Alkylation; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Formates; Indicators and Reagents; Iodoacetamide; Iodoacetic Acid; Oxidation-Reduction; Peptides; Proteins; Pyridines | 2017 |
The Impact of Commonly Used Alkylating Agents on Artifactual Peptide Modification.
Topics: Acetamides; Alkylating Agents; Alkylation; Animals; Artifacts; Cysteine; Iodoacetamide; Male; Methionine; Oxidation-Reduction; Peptides; Protein Processing, Post-Translational; Proteomics; Rats; Testis | 2017 |
Isotopically-Labeled Iodoacetamide-Alkyne Probes for Quantitative Cysteine-Reactivity Profiling.
Topics: Alkynes; Carbon Isotopes; Chromatography, High Pressure Liquid; Cysteine; HeLa Cells; Humans; Iodoacetamide; Nitrogen Isotopes; Oxidation-Reduction; Proteomics; Tandem Mass Spectrometry; Thioredoxins | 2018 |
Proteome-wide analysis of cysteine oxidation reveals metabolic sensitivity to redox stress.
Topics: Adaptation, Physiological; Animals; Cells, Cultured; Cysteine; Fumarate Hydratase; Hydrogen Peroxide; Iodoacetamide; Isotope Labeling; Kidney; Male; Mice; Mice, Knockout; Oxidation-Reduction; Oxidative Stress; Proteome; Proteomics | 2018 |
Cysteine-Selective Phosphonamidate Electrophiles for Modular Protein Bioconjugations.
Topics: Antineoplastic Agents, Immunological; Cysteine; Humans; Immunoconjugates; Iodoacetamide; Maleimides; Organophosphonates; Receptor, ErbB-2; Sulfhydryl Compounds; Trastuzumab | 2019 |
Exploring the nature of inclusion bodies by MALDI mass spectrometry using recombinant proinsulin as a model protein.
Topics: Carbon Isotopes; Cysteine; Cytoplasm; Escherichia coli; Humans; Hydrogen-Ion Concentration; Hydrophobic and Hydrophilic Interactions; Inclusion Bodies; Iodoacetamide; Methionine; Nitrogen Isotopes; Oxidation-Reduction; Peptides; Proinsulin; Protein Domains; Protein Processing, Post-Translational; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds | 2019 |
Profiling Cysteine Reactivity and Oxidation in the Endoplasmic Reticulum.
Topics: Alkynes; Cell Line, Tumor; Cysteine; Endoplasmic Reticulum; Humans; Indicators and Reagents; Iodoacetamide; Lipoylation; Oxidation-Reduction; Proteome; Proteomics; Thapsigargin; Tunicamycin; Unfolded Protein Response | 2020 |
Mechanistic Studies of Cytochrome P450 3A4 Time-Dependent Inhibition Using Two Cysteine-Targeting Electrophiles.
Topics: Alkylation; Apoproteins; Carbon Monoxide; Cysteine; Cytochrome P-450 CYP3A; Cytochrome P-450 CYP3A Inhibitors; Enzyme Assays; Iodoacetamide; Maleimides; Oxidation-Reduction; Protein Conformation; Recombinant Proteins | 2020 |
Design and Evaluation of Artificial Hybrid Photoredox Biocatalysts.
Topics: Acridines; Catalysis; Cysteine; Drug Design; Electron Transport; Iodoacetamide; Models, Molecular; Molecular Structure; Oxidation-Reduction; Oxygenases; Photochemical Processes | 2020 |
Irritant-evoked activation and calcium modulation of the TRPA1 receptor.
Topics: Amino Acid Sequence; Calcium; Cysteine; Electric Conductivity; Humans; Iodoacetamide; Ion Channel Gating; Models, Molecular; Mutation; Oximes; TRPA1 Cation Channel | 2020 |
Cysteine alkylation methods in shotgun proteomics and their possible effects on methionine residues.
Topics: Alkylation; Cysteine; Iodoacetamide; Methionine; Proteomics | 2021 |
A conserved, buried cysteine near the P-site is accessible to cysteine modifications and increases ROS stability in the P-type plasma membrane H+-ATPase.
Topics: Alkylation; Amino Acid Sequence; Amino Acid Substitution; Arabidopsis; Arabidopsis Proteins; Conserved Sequence; Copper; Cysteine; Hydrogen Peroxide; Iodoacetamide; Kinetics; Microsomes; Models, Molecular; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Conformation; Protein Domains; Proton-Translocating ATPases; Reactive Oxygen Species; Recombinant Proteins; Saccharomyces cerevisiae; Sequence Alignment; Sequence Homology, Amino Acid; Structure-Activity Relationship | 2021 |
SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*.
Topics: Biotin; Cycloaddition Reaction; Cysteine; HEK293 Cells; Humans; Iodoacetamide; Ion Mobility Spectrometry; Peptides; Proteomics; Solid-Phase Synthesis Techniques | 2021 |
A detailed mapping of the readily accessible disulphide bonds in the cortex of wool fibres.
Topics: Acrylamide; Alkylation; Amino Acid Sequence; Animals; Chromatography, Liquid; Cysteine; Disulfides; Humans; Iodoacetamide; Iodoacetic Acid; Keratins, Hair-Specific; Peptide Mapping; Protein Isoforms; Protein Multimerization; Sheep, Domestic; Tandem Mass Spectrometry; Wool; Wool Fiber | 2021 |
Optimization of cysteine residue alkylation using an on-line LC-MS strategy: Benefits of using a cocktail of haloacetamide reagents.
Topics: Acetamides; Alkylation; Chromatography, Liquid; Cysteine; Iodoacetamide; Proteins; Tandem Mass Spectrometry | 2021 |
Ribisins and Certain Analogues Exert Neuroprotective Effects through Activation of the Keap1-Nrf2-ARE Pathway.
Topics: Animals; Antioxidants; Biological Products; Cysteine; Heme Oxygenase (Decyclizing); Humans; Iodoacetamide; Kelch-Like ECH-Associated Protein 1; Mice; NAD; Neuroblastoma; Neuroprotective Agents; NF-E2-Related Factor 2; Oxidative Stress; Rats | 2022 |