cysteine and flash-edt2

cysteine has been researched along with flash-edt2 in 18 studies

Research

Studies (18)

TimeframeStudies, this research(%)All Research%
pre-19900 (0.00)18.7374
1990's1 (5.56)18.2507
2000's14 (77.78)29.6817
2010's3 (16.67)24.3611
2020's0 (0.00)2.80

Authors

AuthorsStudies
Adams, SR; Griffin, BA; Tsien, RY1
Adams, SR; Griffin, BA; Jones, J; Tsien, RY1
Beam, KG; Proenza, C; Stroffekova, K1
Adams, SR; Bouwer, J; Deerinck, TJ; Ellisman, MH; Gaietta, G; Laird, DW; Sosinsky, GE; Tour, O; Tsien, RY1
Adams, SR; Campbell, RE; Gross, LA; Llopis, J; Martin, BR; Tsien, RY; Walkup, GK; Yao, Y1
Davis, GW; Marek, KW1
Flohr, NC; Robia, SL; Thomas, DD1
Genisyuerek, S; Langhorst, MF; Stuermer, CA1
Kikuchi, Y; Maeda, M; Nakanishi, J; Takarada, T; Yunoki, S1
Chen, B; Mayer, MU; Negash, S; Squier, TC; Xiong, Y; Yan, P1
Estévez, JM; Somerville, C1
Hearps, AC; Jans, DA; Kuusisto, HV; Piller, SC; Pryor, MJ; Rawlinson, SM1
Bishop, AC; Zhang, XY1
Cappai, R; Coleman, BM; Han, S; Hatters, DM; Hill, AF; Nisbet, RM1
Chen, CC; Hwang, RD; Knecht, DA1
Fuller, AA; Gao, J; Huang, Q; Pace, CJ; Palla, KS; Wang, F1
Fessenden, JD; Mahalingam, M1
Barros, TP; Dobson, CM; Esbjörner, EK; Hanspal, MA; Kumita, JR; Matharu, NS; Ng, JSW; Wilson, MR; Yerbury, JJ1

Other Studies

18 other study(ies) available for cysteine and flash-edt2

ArticleYear
Specific covalent labeling of recombinant protein molecules inside live cells.
    Science (New York, N.Y.), 1998, Jul-10, Volume: 281, Issue:5374

    Topics: Amino Acid Sequence; Calmodulin; Cell Membrane Permeability; Cell Survival; Cysteine; Energy Transfer; Ethylene Glycol; Fluoresceins; Fluorescence; Fluorescent Dyes; Green Fluorescent Proteins; HeLa Cells; Humans; Jurkat Cells; Ligands; Luminescent Proteins; Molecular Sequence Data; Organometallic Compounds; Peptides; Protein Structure, Secondary; Recombinant Proteins; Spectrometry, Fluorescence; Transfection

1998
Fluorescent labeling of recombinant proteins in living cells with FlAsH.
    Methods in enzymology, 2000, Volume: 327

    Topics: Amino Acid Sequence; Animals; Biochemistry; Cell Line; Cysteine; Fluoresceins; Fluorescent Dyes; Humans; Microscopy, Fluorescence; Molecular Sequence Data; Organometallic Compounds; Recombinant Fusion Proteins; Transfection

2000
The protein-labeling reagent FLASH-EDT2 binds not only to CCXXCC motifs but also non-specifically to endogenous cysteine-rich proteins.
    Pflugers Archiv : European journal of physiology, 2001, Volume: 442, Issue:6

    Topics: Amino Acid Sequence; Animals; Binding Sites; Calcium Channels, L-Type; Calmodulin; Cell Line; CHO Cells; Cricetinae; Cysteine; Electric Conductivity; Fluoresceins; Fluorescent Dyes; Humans; Indicators and Reagents; Magnetic Resonance Spectroscopy; Mice; Muscle, Skeletal; Mutagenesis; Oligopeptides; Organometallic Compounds; Polymerase Chain Reaction; Proteins; Recombinant Proteins; Spectrometry, Fluorescence; Transfection

2001
Multicolor and electron microscopic imaging of connexin trafficking.
    Science (New York, N.Y.), 2002, Apr-19, Volume: 296, Issue:5567

    Topics: 3,3'-Diaminobenzidine; Amino Acid Motifs; Animals; Arsenicals; Cell Line; Cell Membrane; Connexin 43; Cysteine; Endocytosis; Exocytosis; Fluoresceins; Fluorescence; Fluorescent Dyes; Gap Junctions; HeLa Cells; Humans; Microscopy, Confocal; Microscopy, Electron; Microscopy, Immunoelectron; Organometallic Compounds; Oxazines; Patch-Clamp Techniques; Protein Transport; Recombinant Proteins; Transport Vesicles

2002
New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications.
    Journal of the American Chemical Society, 2002, May-29, Volume: 124, Issue:21

    Topics: Amino Acid Motifs; Amino Acid Sequence; Anisotropy; Cysteine; Electrophoresis, Polyacrylamide Gel; Fluoresceins; Fluorescence Polarization; Fluorescent Dyes; Fluorometry; HeLa Cells; Humans; Molecular Sequence Data; Organometallic Compounds; Recombinant Proteins; Spectrometry, Fluorescence

2002
Transgenically encoded protein photoinactivation (FlAsH-FALI): acute inactivation of synaptotagmin I.
    Neuron, 2002, Dec-05, Volume: 36, Issue:5

    Topics: Amino Acid Motifs; Animals; Calcium; Calcium-Binding Proteins; Cysteine; Drosophila melanogaster; Drosophila Proteins; Electrophysiology; Epitopes; Female; Fluoresceins; Indicators and Reagents; Light; Male; Membrane Fusion; Membrane Glycoproteins; Membrane Potentials; Nerve Tissue Proteins; Neurons; Neurotransmitter Agents; Organometallic Compounds; Recombinant Fusion Proteins; Synapses; Synaptic Transmission; Synaptic Vesicles; Synaptotagmin I; Synaptotagmins; Transgenes

2002
Phospholamban pentamer quaternary conformation determined by in-gel fluorescence anisotropy.
    Biochemistry, 2005, Mar-22, Volume: 44, Issue:11

    Topics: Arsenicals; Calcium-Binding Proteins; Cysteine; Cytoplasm; Electrophoresis, Polyacrylamide Gel; Fluorescein-5-isothiocyanate; Fluoresceins; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Models, Molecular; Mutagenesis, Site-Directed; Organometallic Compounds; Oxazines; Protein Structure, Quaternary; Protein Structure, Tertiary; Serum Albumin, Bovine

2005
Accumulation of FlAsH/Lumio Green in active mitochondria can be reversed by beta-mercaptoethanol for specific staining of tetracysteine-tagged proteins.
    Histochemistry and cell biology, 2006, Volume: 125, Issue:6

    Topics: Cysteine; Fluoresceins; Fluorescent Dyes; HeLa Cells; Humans; Membrane Proteins; Mercaptoethanol; Microscopy, Fluorescence; Mitochondria; Organometallic Compounds; Repetitive Sequences, Amino Acid; Staining and Labeling

2006
FRET-based monitoring of conformational change of the beta2 adrenergic receptor in living cells.
    Biochemical and biophysical research communications, 2006, May-19, Volume: 343, Issue:4

    Topics: Adrenergic beta-2 Receptor Agonists; Adrenergic beta-2 Receptor Antagonists; Amino Acid Sequence; Cell Line; Cysteine; Fluoresceins; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Green Fluorescent Proteins; Humans; Isoproterenol; Molecular Sequence Data; Organometallic Compounds; Propanolamines; Protein Conformation; Receptors, Adrenergic, beta-2; Recombinant Proteins

2006
Fluorophore-assisted light inactivation of calmodulin involves singlet-oxygen mediated cross-linking and methionine oxidation.
    Biochemistry, 2006, Apr-18, Volume: 45, Issue:15

    Topics: Amino Acid Sequence; Animals; Calmodulin; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Cysteine; Fluoresceins; Fluorescent Dyes; Histidine; Kinetics; Light; Mass Spectrometry; Methionine; Mice; Molecular Sequence Data; Myosin-Light-Chain Kinase; Organometallic Compounds; Oxidation-Reduction; Peptide Fragments; Peptides; Protein Structure, Tertiary; Rabbits; Reactive Oxygen Species; Ryanodine Receptor Calcium Release Channel; Singlet Oxygen; Trypsin

2006
FlAsH-based live-cell fluorescent imaging of synthetic peptides expressed in Arabidopsis and tobacco.
    BioTechniques, 2006, Volume: 41, Issue:5

    Topics: Arabidopsis; Cysteine; Fluoresceins; Fluorescent Dyes; Microscopy, Confocal; Nicotiana; Organometallic Compounds; Recombinant Proteins; Repetitive Sequences, Amino Acid

2006
The biarsenical dye Lumio exhibits a reduced ability to specifically detect tetracysteine-containing proteins within live cells.
    Journal of fluorescence, 2007, Volume: 17, Issue:6

    Topics: Amino Acid Motifs; Amino Acid Sequence; Arsenicals; Cysteine; Fluoresceins; Fluorescent Dyes; Green Fluorescent Proteins; HeLa Cells; Humans; Organometallic Compounds; Oxazines; Proteins; Recombinant Fusion Proteins; Sensitivity and Specificity; Spectrometry, Fluorescence

2007
Engineered inhibitor sensitivity in the WPD loop of a protein tyrosine phosphatase.
    Biochemistry, 2008, Apr-15, Volume: 47, Issue:15

    Topics: Catalysis; Cysteine; Enzyme Inhibitors; Fluoresceins; Mutagenesis, Insertional; Organometallic Compounds; Phosphopeptides; Point Mutation; Protein Conformation; Protein Engineering; Protein Tyrosine Phosphatase, Non-Receptor Type 2

2008
Conformational detection of prion protein with biarsenical labeling and FlAsH fluorescence.
    Biochemical and biophysical research communications, 2009, Mar-13, Volume: 380, Issue:3

    Topics: Alzheimer Disease; Animals; Cysteine; Fluoresceins; Fluorescence; Huntington Disease; Luminescent Measurements; Mice; Neurodegenerative Diseases; Organometallic Compounds; Parkinson Disease; Prion Diseases; Protein Folding; Protein Structure, Secondary; PrPC Proteins; Recombinant Proteins

2009
ReAsH: another viable option for in vivo protein labelling in Dictyostelium.
    Journal of microscopy, 2009, Volume: 234, Issue:1

    Topics: Animals; Arsenicals; Contractile Proteins; Cysteine; Dictyostelium; Filamins; Fluoresceins; Fluorescence; Microfilament Proteins; Organometallic Compounds; Oxazines; Protozoan Proteins; Staining and Labeling

2009
A FlAsH-tetracysteine assay for quantifying the association and orientation of transmembrane α-helices.
    Chembiochem : a European journal of chemical biology, 2011, May-02, Volume: 12, Issue:7

    Topics: Cell Membrane; Cysteine; Fluoresceins; Fluorescence; Membrane Proteins; Micelles; Organometallic Compounds; Protein Structure, Secondary

2011
Site-specific labeling of the type 1 ryanodine receptor using biarsenical fluorophores targeted to engineered tetracysteine motifs.
    PloS one, 2013, Volume: 8, Issue:5

    Topics: Amino Acid Motifs; Amino Acid Sequence; Arsenicals; Cysteine; Dimercaprol; Fluoresceins; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; HEK293 Cells; Humans; Molecular Sequence Data; Organometallic Compounds; Oxazines; Protein Engineering; Ryanodine Receptor Calcium Release Channel; Staining and Labeling

2013
Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.
    Biochemistry, 2019, 10-01, Volume: 58, Issue:39

    Topics: Amino Acid Sequence; Amyotrophic Lateral Sclerosis; Cell Line, Tumor; Cell Nucleus; Cysteine; Cytosol; DNA-Binding Proteins; Electroporation; Fluoresceins; Fluorescent Dyes; Frontotemporal Lobar Degeneration; Humans; Kinetics; Models, Biological; Optical Imaging; Organometallic Compounds; Protein Transport; Sequence Tagged Sites; Time-Lapse Imaging; Transfection

2019