cysteine has been researched along with dithiothreitol in 782 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 233 (29.80) | 18.7374 |
| 1990's | 235 (30.05) | 18.2507 |
| 2000's | 233 (29.80) | 29.6817 |
| 2010's | 77 (9.85) | 24.3611 |
| 2020's | 4 (0.51) | 2.80 |
| Authors | Studies |
|---|---|
| Creighton, TE | 3 |
| Bazer, FW; Roberts, RM; Sander, EG; Schlosnagle, DC | 1 |
| Craven, PA; DeRubertis, FR | 1 |
| Glass, DB; Goldberg, ND; Haddox, MK; Holmes-Gray, B; Moser, ME; Stephenson, JH; White, JG | 1 |
| Schellenberger, A; Ulbrich, R | 1 |
| Bernheim, F | 1 |
| Kuo, SC; Lampen, JO | 1 |
| Chan, PC; Kindya, RJ | 1 |
| Porter, M; Weber, A | 1 |
| Leibach, FH; Mendicino, J; Pillion, DJ; Von Tersch, FJ | 1 |
| Palmer, FB | 1 |
| Sato, M; Sato, T; Sawada, M | 1 |
| Chang, TM; Dazord, A; Neville, DM | 1 |
| Kobayashi, GS; Maresca, B; Medoff, G; Schlessinger, D | 1 |
| Krzystyniak, K; Roszkowski-Sliź, W; Ryzewski, J | 2 |
| Bertone, G; Dianzani, MU | 1 |
| Bockaert, J; Hanoune, J; Lucas, M | 1 |
| Hartmann, GR; Neudecker, TJ | 1 |
| Dumont, JE; Pisarev, MA | 1 |
| Chatagner, F; Portemer, C | 1 |
| Hori, S | 1 |
| Kako, KJ; Liu, MS | 1 |
| Reitz, RC; Thompson, JA | 1 |
| Clinkenbeard, KD; Lane, MD; Miziorko, HM; Reed, WD | 1 |
| Fitzgerald, TJ; Johnson, RC; Miller, JN; Sykes, JA | 1 |
| Fontan, E; Truffa-Bachi, P | 1 |
| Fitzgerald, TJ; Miller, JN; Norris, SJ; Sykes, JA | 1 |
| Chalmers, WS; Taylor-Robinson, D | 1 |
| Barran, LR; Schneider, EF | 1 |
| Pourreau-Schneider, N | 1 |
| Arvidsson, A; Hagenfeldt, L; Larsson, A | 1 |
| Guikema, JA; Yocum, CF | 1 |
| Finlay, TH; Johnson, AJ; Marcus, DL | 1 |
| Murray, PR | 1 |
| Azocar, O; Muñoz, E | 1 |
| Kallistratos, G | 1 |
| Hayaishi, O; Miyamoto, T; Ogino, N; Yamamoto, S | 1 |
| Kamataki, T; Kitagawa, H; Ozawa, N; Sugita, O | 1 |
| Cline, MJ; Toohey, JI | 1 |
| Agarwal, HC; Nair, AM | 1 |
| Mickelson, MN | 1 |
| Nakayasu, M; Nishiuch, Y; Oikawa, A; Sasaki, M | 1 |
| Berry, RW; Weisblat, DA | 1 |
| Chuang, J; Zull, JE | 1 |
| D'Agostino, MA; Kalf, GF; Lowry, KM | 1 |
| Dinovo, EC; Miyada, DS; Nakamura, RM | 1 |
| Ishimoto, Y; Kasahara, K; Ubuka, T | 1 |
| Azuma, T; Hamaguchi, K; Kishida, F | 1 |
| Inatomi, K; Slaughter, JC | 1 |
| Mahi, CA; Yanagimachi, R | 1 |
| Blair, EB; Fishman, DL; Heatley, GJ; Tull, AH | 1 |
| Gravela, E | 1 |
| Dawson, RM; Hazlewood, GP | 1 |
| Hon, RB; Kaneko, M; Kobayashi, A; Masuda, H; Yamazaki, N; Yuan, G | 1 |
| Felton, JR; Hoover, CI; Ng, CY | 1 |
| Bonet, ML; Cadenas, E; Llorca, FI | 1 |
| Anderson, S; Falick, AM; Kosen, PA; Kuntz, ID; Marks, CB | 1 |
| Björk, I; Ylinenjärvi, K | 1 |
| Feng, Y; Forgac, M | 2 |
| Schwille, PO; Sharma, V | 1 |
| Aw, TY; Williams, MW | 1 |
| Chen, Q; Stevens, JL; Yu, K | 1 |
| Eisenbart, J; Fless, GM; Hinman, J; Makino, K; Pfaffinger, D; Scanu, AM | 1 |
| Cheatham, B; Kahn, CR | 1 |
| Chen, Q; Holbrook, NJ; Stevens, JL; Yu, K | 1 |
| Gradehandt, G; Hampl, J; Kalbacher, H; Rüde, E | 1 |
| Ivancheva, E; Liochev, S | 1 |
| Cuccaro, ML; Jacobs, LS; Lorenson, MY | 1 |
| Flatmark, T; Haavik, J; Martínez, A; Olafsdottir, S | 1 |
| Fujimoto, H; Haneda, M; Nakatsuka, M; Nisimoto, Y; Nomura, S; Ohta, T; Shibata, Y; Takeuchi, F; Tamai, T; Tsubouchi, R | 1 |
| Kimura, M; Koizumi, S; Otsuka, F | 1 |
| Kainosho, M; Miyake, Y; Uchida, K | 1 |
| Borges, KM; Boswell, JS; Liebross, RH; Wetterhahn, KE | 1 |
| Chen, SA; Rutherfurd, KJ; Shively, JE | 1 |
| Benson, BJ; Griffin, PR; Lesikar, DD; Moffat, B; Naidu, A; Stults, JT | 1 |
| Driessen, AJ; Kaback, HR; Konings, WN; Menick, DR; van Iwaarden, PR | 1 |
| Del Boccio, G; Federici, G; Lo Bello, M; Pennelli, A; Petruzzelli, R; Ricci, G; Whitehead, EP | 1 |
| Bannister, AJ; Cook, A; Kouzarides, T | 1 |
| Liedtke, AJ; Nellis, SH; Renstrom, B | 1 |
| Denton, ME; Scheraga, HA | 1 |
| Birwé, H; Hesse, A | 1 |
| Balhorn, R; Corzett, M; Mazrimas, J; Watkins, B | 1 |
| Karlström, AR; Levine, RL | 1 |
| Brüne, B; Lapetina, EG | 1 |
| Hiraoka, Y; Ikeguchi, M; Kuwajima, K; Sugai, S; Sugawara, T | 1 |
| Cieplak, W; Feron, C; Keith, JM; Lobet, Y; Locht, C | 1 |
| Caron, MG; DeBlasi, A; Dohlman, HG; Frielle, T; Lefkowitz, RJ | 1 |
| Chetty, CS; McBride, V; Rajanna, B; Rajanna, S | 1 |
| de Jong, MD; van Hoek, AN; van Os, CH | 1 |
| Hartman, FC; Porter, MA; Potter, MD | 1 |
| Birkett, DJ; Lelo, A; Miners, JO | 1 |
| Brown, PC; Chen, Q; Jones, TW; Stevens, JL | 1 |
| Brodie, AE; Reed, DJ | 1 |
| DeFulvio, JD; Thakur, ML | 1 |
| Kuzuhara, T; Matsuyama, K; Nakajima, Y; Natori, S | 1 |
| Agathos, SN; Demain, AL | 1 |
| Takahashi, K; Takita, T; Umezawa, H | 1 |
| Keleti, T; Leoncini, R; Marinello, E; Pagani, R | 1 |
| Naish, S; Riley, PA | 1 |
| Claiborne, A; Poole, LB | 1 |
| Bhatnagar, A; Das, B; Liu, SQ; Srivastava, SK | 1 |
| Koyano, H; Yamashita, T | 1 |
| Ahmad, S; Daniel, EE | 1 |
| Butler, J; Land, EJ; Prütz, WA; Swallow, AJ | 1 |
| Dennis, MF; Stratford, MR; Wardman, P; Watfa, RR | 1 |
| Bast, A; Haenen, GR; Timmerman, H; Vermeulen, NP | 1 |
| Douglas, KT; John, DC | 1 |
| Ballou, DP; Distefano, MD; Massey, V; Miller, SM; Moore, MJ; Walsh, CT; Williams, CH | 1 |
| Aberg, A; Ahgren, A; Hahne, S; Karlsson, M; Larsson, A; Ormö, M; Sjöberg, BM | 1 |
| Singh, K | 1 |
| Dalton, JP; Heffernan, M | 1 |
| Altamirano, MM; Calcagno, M; Mulliert, G | 1 |
| Hartman, FC; Porter, MA | 1 |
| Biessen, EA; Horn, AS; Norder, JA; Robillard, GT | 1 |
| Abarzua, M; Davis, JA; Rajan, T; Weinberg, JM | 1 |
| Fahey, RC; Fenton, SS | 1 |
| Fujise, N; Nishimura, M; Yagasaki, O | 1 |
| Lefkowitz, RJ; Nakata, H; Regan, JW | 1 |
| Lobban, MD; van Heyningen, S | 1 |
| Alberta, JA; Fisher, RR; Modrak, DE; Wu, LN | 1 |
| Altland, K; Becher, P; Bjellqvist, B; Rossmann, U | 1 |
| Gallin, JI; Nath, J | 1 |
| Chávez, E; Holguín, JA | 1 |
| Goto, T; Ishikawa, K; Isobe, M; Nagahama, Y; Yoshikuni, M | 1 |
| Decottignies, P; Gadal, P; Jacquot, JP; Le Maréchal, P; Miginiac-Maslow, M; Schmitter, JM | 1 |
| Neujahr, HY | 1 |
| May, JM | 1 |
| Maher, PA; Singer, SJ | 1 |
| Brockway, RA; Nakatani, M; Paglia, DE; Toohey, JI; Valentine, WN | 1 |
| Helgestad, J; Lie, SO; Storm-Mathisen, I | 1 |
| Powers, DB; Wells, JA | 1 |
| Barr, PJ; Gibson, HL; Hallewell, RA; Matheson, NR; Travis, J | 1 |
| Snyder, GH | 1 |
| Tampo, Y; Yonaha, M | 1 |
| Kitamura, S; Tatsumi, K | 1 |
| Beuzard, Y; Caburi-Martin, J; Domenget, C; Galacteros, F; Garel, MC; Prehu, C | 1 |
| Busse, D; Helbig, I; Pohl, B | 1 |
| Fukui, S; Ichikawa, M; Kakizono, T; Nihira, T; Taguchi, H; Toraya, T; Yasuda, T | 1 |
| López-Barea, J; Mata, AM; Pinto, MC | 1 |
| Clapper, DL; Davis, JA; Epel, D; Lamothe, PJ; Patton, C | 1 |
| Colman, RF; Tomich, JM | 1 |
| Adamson, L; Blombäck, B; Hessel, B; Procyk, R | 1 |
| Goodearl, AD; Moreton, K; Williams, J | 1 |
| Gibson, DD; Hawrylko, J; McCay, PB | 1 |
| Apffel, CA; Nathanson, IT; Walker, JE | 1 |
| Funaba, T; Izaki, K; Tashiro, Y | 1 |
| Chapman, JD; Greenstock, CL; Raleigh, JA; Reuvers, AP; Shiermen, E | 1 |
| Abrams, WR; Schiff, JA; Schmidt, A | 1 |
| Chapuis, RM; Koshland, ME | 1 |
| Botes, DP | 1 |
| Klee, CB | 1 |
| Dancewicz, AM; Sopata, I | 1 |
| Marzluf, GA | 1 |
| Horecker, BL; Nakashima, K | 1 |
| Halprin, KM; Levine, V; Okawara, A | 1 |
| Brown, SI; Bull, FG; Hook, CW; Iwanij, V | 1 |
| Abadiedebat, J; de Recondo, AM; Rossignol, JM; Tillit, J | 1 |
| Lukes, RJ; O'Brien, RL; Paolilli, P; Parker, JW; Steiner, J | 1 |
| Fleisch, JH; Krzan, MC; Titus, E | 1 |
| Allsop, J; Watts, RW; Westwick, WJ | 1 |
| Hartzell, RW; Neurath, AR; Rubin, BA; Vernon, SK | 1 |
| Natelson, S; Sasaki, M; Schultz, A | 1 |
| Catt, KJ; Dufau, ML; Ryan, D | 1 |
| Fan, CC; Plaut, GW | 1 |
| Katsui, G; Kawai, K; Nakao, M; Nakao, T | 1 |
| Bernhard, S; Seydoux, F | 1 |
| Abou-Issa, H; Mendicino, J | 1 |
| Ellis, G; Goldberg, DM | 1 |
| Stokes, GB; Stumpf, PK | 1 |
| Buecher, EJ; Hansen, EL; Perez-Mendez, G; Yarwood, E | 1 |
| Hassall, H; Soutar, AK | 1 |
| Church, RL; Tanzer, ML; Yaeger, JA | 1 |
| Adams, JB; Low, J | 1 |
| Akazawa, T; Nishimura, M | 1 |
| Cain, RB; Houghton, C; Wright, KA | 1 |
| O'Malley, KL; Terwilliger, RC | 1 |
| Adams, JB; Ellyard, RK; Low, J | 1 |
| Larmas, M | 2 |
| Dierich, MP; Ferrone, S; Pellegrino, MA; Reisfeld, RA | 1 |
| Carlisky, NJ; Sadnik, IL | 1 |
| Kurihara, T; Takahashi, Y | 1 |
| Bernstein, RS; Schraer, R | 1 |
| Truscoe, R; Turner, JC | 1 |
| Click, RE; Heber-Katz, E | 1 |
| Braunsberg, H; Killen, E; Richardson, SG | 1 |
| Fenselau, A; Moore, J | 1 |
| Bessman, SP; Geiger, PJ | 1 |
| Burger, PE; Dowdle, EB; Wilson, EL | 1 |
| Kallistratos, G; Malorny, G | 1 |
| Etherington, DJ | 1 |
| Bouma, JM; Gruber, M; Huisman, W | 1 |
| Kawachi-Takahashi, S; Kohno, H; Takahashi, M; Tanaka, K | 1 |
| Toda, N | 1 |
| Chang, M; Ferrendelli, JA; Johnson, EM; Needleman, P | 1 |
| Kanatani, H; Kishimoto, T | 1 |
| Doinel, C; Ropars, C; Salmon, C | 1 |
| Finkelstein, JD; Harris, B | 1 |
| Cannella, C; Federici, G; Santoro, L; Tomati, U | 1 |
| Gunsalus, IC; Yu, C | 1 |
| Boer, GJ | 1 |
| Cornell, JS; Pierce, JG | 1 |
| Page, WJ; Stock, JJ | 1 |
| Maluszyńska, GM; Pietrazek, A | 1 |
| Nakanishi, S; Natelson, S; Takahara, K | 1 |
| Fujita, T; Oata, M; Orimo, H; Yoshikawa, M | 1 |
| Madsen, A | 1 |
| Deutscher, MP | 1 |
| Blackburn, MN; Chirgwin, JM; James, GT; Kempe, TD; Noltmann, EA; Parsons, T; Register, AM; Schnackerz, KD | 1 |
| Knuuttila, ML; Mäkinen, KK | 1 |
| Warren, WA | 1 |
| Lukert, PD | 1 |
| Cornell, NW; Crivaro, KE | 1 |
| Arnold, WN | 1 |
| D'Souza, L; Freisheim, JH; Warwick, PE | 1 |
| Jerry, LM; Kunkel, HG | 1 |
| McIntosh, ME; Watt, DD | 1 |
| Mäkinen, KK; Mäkinen, PL | 1 |
| Buchwald, BM | 1 |
| Askonas, BA; Parkhouse, RM | 1 |
| Lightowler, JE; Lightowler, NM | 1 |
| Dinamarca, ML; Levenbook, L; Valdés, E | 1 |
| Marchant, RH | 1 |
| Dolder, F; Isliker, H; Jacot-Guillarmod, H; Pelet, BP | 1 |
| Acar, JF; Cayeux, P; Chabbert, YA | 1 |
| Paunio, IK; Paunio, KU | 1 |
| Bloemendal, H; Matze, R; Schoenmakers, JG; Van Poppel, M | 1 |
| Biaglow, JE; Gerweck, LE; Issels, RW; Jacobson, B; Mitchell, JB; Russo, A; Varnes, ME | 1 |
| Barry, BK; Edwards, JC; Gruetter, CA; Gruetter, DY; Ignarro, LJ | 1 |
| Baricos, WH; Barry, BK; Edwards, JC; Gruetter, CA; Gruetter, DY; Ignarro, LJ; Ohlstein, EH | 1 |
| Heinonen, J; Lahti, R | 1 |
| Di Cola, D; Federici, G | 1 |
| Famaey, JP; Fontaine, J; Reuse, J | 1 |
| Johnson, HM | 1 |
| Freedman, JH; Horwitz, SB; Peisach, J | 1 |
| Chua, BH; Giger, KE; Kleinhans, BJ; Morgan, HE; Robishaw, JD | 1 |
| Fullmer, CS; McCarty, RE; Moroney, JV | 1 |
| Esaki, N; Karai, N; Soda, K; Tanaka, H | 1 |
| Fabregat, I; Machado, A; Satrústegui, J | 1 |
| Schaffer, SW; Smith, GK | 1 |
| Diamond, LS; Gillin, FD | 1 |
| Britvan, LJ; Hiserodt, JC; Targan, SR | 1 |
| Aust, SD; Bucher, JR; Tien, M | 1 |
| Barnea, A; Cho, G | 1 |
| Harrop, HA; Held, KD; Michael, BD | 1 |
| Burnell, JN; Karle, JA; Shrift, A | 1 |
| Hayashi, S; Kameji, T; Murakami, Y | 1 |
| Morrison, M; Nakamura, M; Nakamura, S; Yamazaki, I | 1 |
| Chapman, CB; Mitchell, GF | 1 |
| Bear, JL; Howard, RA; Kimball, AP; Miller, JA; Serio, GF | 1 |
| Juliao, SF; Regen, DM; Schraw, WP; Tarpley, HL | 1 |
| Gershon, H; Shanks, L | 1 |
| Alper, CA; Babior, BM; Rosen, FS; Rutherford, CJ | 1 |
| Asano, M; Hidaka, H | 1 |
| Beamish, RE; Dhalla, NS; Singal, PK; Yates, JC | 1 |
| Litt, M; Marriott, C; Martin, R | 1 |
| Holmström, E; Johnson, U | 1 |
| Dhople, AM; Hanks, JH | 1 |
| Jung, CY; Mookerjee, BK | 1 |
| Anderson, R; Jones, PT | 1 |
| Berkich, D; Neely, JR; Robishaw, JD | 1 |
| Verdery, RB | 1 |
| Hillson, DA | 1 |
| Ozaki, H; Shiio, I | 1 |
| Annamalai, AE; Colman, RF | 1 |
| Cain, K; Skilleter, DN | 1 |
| Ascoli, D; Hughes, J; Joshi, S | 1 |
| Hsieh, DP; Wan, NC | 1 |
| Loor, F | 1 |
| Jörnvall, H; Philipson, L | 1 |
| Jones, GA; Pickard, MD | 1 |
| Gupta, CM; Khorana, HG; Takagaki, Y | 1 |
| Bennett, N; Ildefonse, M; Serre, V | 1 |
| Ballatori, N; Truong, AT | 1 |
| Eckardt, S; Köhr, G; Lüddens, H; Monyer, H; Seeburg, PH | 1 |
| Chen, HS; Escobar, W; Heinemann, SF; Lipton, SA; Sullivan, JM; Traynelis, SF | 1 |
| Katiyar, SS; Sheikh, S | 2 |
| Hemingway, J; Jayawardena, KG | 1 |
| Gershengorn, MC; Nussenzveig, DR; Perlman, JH; Wang, W | 1 |
| Hawrot, E; McLaughlin, JT; Yellen, G | 1 |
| Glockshuber, R; Hennecke, H; Loferer, H; Wunderlich, M | 1 |
| Hellekant, G; Markley, JL; Ming, D | 1 |
| Bockaert, J; Glowinski, J; Marin, P; Maus, M; Prémont, J | 1 |
| Diaz, I; Garcia-Olmedo, F; Piñeiro, M; Rodriguez-Palenzuela, P; Titarenko, E | 1 |
| Arvan, P; Huang, XF | 1 |
| Case, ST; Correia, JJ; Smith, SV | 1 |
| Dirr, H; Sluis-Cremer, N | 1 |
| Lei, Y; Liu, HW; Ploux, O; Vatanen, K | 1 |
| Hendrickson, TL; Imperiali, B; Pathak, R | 1 |
| Heilmeyer, LM; Meyer, H; Shoshan-Barmatz, V; Varsanyi, M; Weil, S | 1 |
| Koch-Brandt, C; Lösch, A | 1 |
| Arnone, MI; Di Lauro, R; Zannini, M | 1 |
| Kil, JO; Kim, GN; Park, I | 1 |
| Elling, L | 1 |
| Dröge, W; Galter, D; Mihm, S | 1 |
| Bilan, PJ; Yip, CC | 1 |
| Hong, JL; Hsiao, CD; Hsieh, CH; Liu, LF; Tam, MF; Tsai, SP; Wang, LY | 1 |
| Arvan, P; Kim, PS | 1 |
| Bravo, C; Chávez, E; Corona, N; Pichardo, J; Reyes-Vivas, H; Zazueta, C | 1 |
| Terada, T | 1 |
| Freeman, JA; Hess, DT; Lin, LH; Norden, JJ | 1 |
| Block, MA; Douce, R; Joyard, J; Maréchal, E; Miège, C | 1 |
| Lehrer, SS | 1 |
| Boelens, R; Crielaard, W; Devreese, B; Düx, P; Hård, K; Hellingwerf, KJ; Hoff, WD; Kaptein, R; Nugteren-Roodzant, IM; van Beeumen, J | 1 |
| Cornelis, S; Devos, R; Foley, LH; Guisez, Y; Plaetinck, G; Scheffler, JE; Tavernier, J; van der Heyden, J | 1 |
| Chae, HZ; Chung, SJ; Rhee, SG | 1 |
| Hirose, M; Takahashi, N; Tatsumi, E | 1 |
| Eckstein, JW; Finer-Moore, J; Foster, PG; Santi, DV; Wataya, Y | 1 |
| Hewlins, MJ; Roy, AB | 1 |
| Castelhano, AL; Copp, L; Feng, R; Krantz, A; Li, B; Stahl, M; Yuan, Z | 1 |
| Doi, K; Fukui, K; Kaneda, M; Takahashi, E; Washio, M | 1 |
| Arnold, SA; Birckbichler, PJ; Carter, HA; Fraij, BM; Lee, KN; Patterson, MK; Takeuchi, Y | 1 |
| Chirgwin, JM; Horowitz, PM; Miller-Martini, DM | 1 |
| Cooper, L; Creighton, TE; Zapun, A | 1 |
| Graham, RM; Karnik, SS; Noda, K; Saad, Y | 1 |
| Devi, BG; Karunakaran, T | 1 |
| Andrisano, V; Bonazzi, D; Cavrini, V; Di Pietra, AM; Gotti, R | 1 |
| Backer, J; Böhlen, P; Decker, MM; Dougher-Vermazen, M; Fairhurst, JL; Hulmes, JD; Kovesdi, I; Seddon, AP | 1 |
| Diaz, I; García-Olmedo, F; Piñeiro, M | 1 |
| Calvete, JJ; Einspanier, R; Karg, H; Klostermeyer, H; Krause, I; Töfper-Petersen, E | 1 |
| Findlay, G; Hirst, TR | 1 |
| Bhardwaj, L; Ramawat, KG | 1 |
| Inoue, H; Ohtsuka, E; Shimizu, M | 1 |
| Giedroc, DP; Kodadek, T; Qiu, H | 1 |
| Christensen, T; Jespersen, AM; Klausen, NK; Nielsen, F; Sørensen, HH | 1 |
| Aniya, Y; Daido, A | 1 |
| McGinnis, K; Wilson, DB | 2 |
| Kirley, TL; Treuheit, MJ | 1 |
| Kong, N; Lodish, HF | 1 |
| Camici, M; Cappiello, M; Del Corso, A; Mura, U | 1 |
| Janáky, R; Oja, SS; Saransaari, P; Varga, V | 1 |
| Fujioka, H; Horiike, K; Ishida, T; Kinoshita, M; Nozaki, M; Takahashi, M | 1 |
| Jespersen, S; Roepstorff, P; Talbo, G | 1 |
| Fiskerstrand, T; Kvalheim, G; Refsum, H; Ueland, PM | 1 |
| Haas, MJ; Joerger, RD | 1 |
| Castro, B; Chiche, L; el Hajji, M; Heitz, A; Le-Nguyen, D | 1 |
| Berggren, PO; Islam, MS; Larsson, O | 1 |
| Galindo, JD; Gómez-Skarmeta, JL; Lozano, JA; Peñafiel, R | 1 |
| Gomi, S; Imae, Y; Iwama, T; Lee, L | 1 |
| Malorni, A; Marino, G; Nitti, G; Pucci, P; Ruoppolo, M; Valsasina, B | 1 |
| Lin, JK; Lin, SJ; Liu, JY | 1 |
| Evans, BR; Herrmann, PC; Tate, J; Woodward, J | 1 |
| Buckley, JT; Hardie, KR; Parker, MW; Schulze, A | 1 |
| Jurman, ME; Liu, Y; Yellen, G | 1 |
| Aposhian, HV; Maiorino, RM; Xu, ZF | 1 |
| Connolly, DT; Glenn, K; Heuvelman, D | 1 |
| Dawes, IW; Grant, CM; MacIver, FH | 1 |
| Maley, F; Reddy, A | 1 |
| Bénitah, JP; Marban, E; Tomaselli, GF | 1 |
| Bigelow, DJ; Hühmer, AF; Schöneich, C; Viner, RI | 1 |
| Jacquier-Sarlin, MR; Polla, BS | 1 |
| Cook, JV; Eidne, KA; Lee, T; McGregor, A; Milligan, G | 1 |
| Eddes, JS; Moritz, RL; Reid, GE; Simpson, RJ | 1 |
| Ameyibor, E; Fu, W; Gardet-Salvi, L; Johnson, MK; Knaff, DB; Schürmann, P; Staples, CR; Stritt-Etter, AL | 1 |
| Gravitt, KR; O'Brian, CA; Ward, NE | 1 |
| Gasdaska, JR; Montfort, WR; Powis, G; Weichsel, A | 1 |
| Anderson, LE; Huppe, HC; Li, AD; Stevens, FJ | 1 |
| Haniu, M; Hui, JO; Katta, V; Le, J; Rohde, MF; Rosenfeld, R | 1 |
| Chu, Y; Lee, EY; Reimann, EM; Schlender, KK; Wilson, SE | 1 |
| Harris, C; Hiranruengchok, R | 1 |
| Chen, Q; Lentz, BR; Lord, ST | 1 |
| Oswald, RE; Wo, ZG | 1 |
| Kreimer, DI; Shin, I; Shnyrov, VL; Silman, I; Villar, E; Weiner, L | 1 |
| Dost, MH; Kok, J; Venema, G; Venema, K | 1 |
| de Gómez-Puyou, MT; Garza-Ramos, G; Gómez-Puyou, A; Pérez-Montfort, R; Rojo-Domínguez, A | 1 |
| Iznaga Escobar, N; Morales, A; Núñez, G | 1 |
| Herscovics, A; Lipari, F | 1 |
| Amitay, R; Bar-Nun, S; Elkabetz, Y; Samuelov, M; Shachar, I; Winitz, D | 1 |
| Casey, PJ; Fu, HW; Moomaw, CR; Moomaw, JF | 1 |
| Lamb, GD; Posterino, GS | 1 |
| Barnett, BL; Bauer, MD; Erwin, CR; Keough, TW; Lacey, MP; Sun, Y; Wang, ES | 1 |
| Ikuta, K; Kameoka, M; Kimura, T; Okada, Y; Tobiume, M | 1 |
| Chadwick, MP; May, FE; Westley, BR | 1 |
| Guzzo, A; Lee, MH; Oda, K; Walker, GC | 1 |
| Guzzo, A; Lee, MH; Walker, GC | 1 |
| Kruidering, M; Nagelkerke, JF; Van de Water, B | 1 |
| Baba, K; Odani, S; Sato, T; Takahashi, Y; Uchiumi, T | 1 |
| Rajalakshmi, M; Sivashanmugam, P | 1 |
| Buè, MC; Ciriolo, MR; De Vito, P; Garaci, E; Incerpi, S; Lafavia, E; Palamara, AT; Rotilio, G | 1 |
| Cherfils, J; Chueca, A; Jacquot, JP; Lemaire, S; Lopez-Gorge, J; Lopez-Jaramillo, J; Miginiac-Maslow, M | 1 |
| Karran, G; Legge, M | 1 |
| Le Gouill, C; Parent, JL; Rola-Pleszczynski, M; Stanková, J | 1 |
| Giusti, L; Lucacchini, A; Mazzoni, MR; Rossi, E; Taddei, S | 1 |
| Gorren, AC; Mayer, B; Schmidt, K; Schrammel, A | 1 |
| Chueh, PJ; DeHahn, T; Morré, DJ; Morré, DM; Penel, C | 1 |
| Fonnum, F; Mathisen, GA; Paulsen, RE | 1 |
| Burstein, Y; Peretz, M; Weiner, LM | 1 |
| Capeyrou, R; Dietrich, G; Emorine, LJ; Gaibelet, G | 1 |
| Cook, JV; Eidne, KA | 1 |
| Bialek, C; Bollen, A; De Neef, P; Di Paolo, E; Robberecht, P; Vilardaga, JP; Waelbroeck, M | 1 |
| Cynshi, O; Ikeda, S; Kushima, Y; Sugawa, M; Takashima, Y | 1 |
| Jiang, M; Tseng, GN; Yao, JA | 1 |
| Díaz, M; Riquelme, G; Sepúlveda, FV | 1 |
| Fahrenkrug, J; Knudsen, SM; Tams, JW; Wulff, BS | 1 |
| Halleck, MM; Liu, H; North, J; Stevens, JL | 1 |
| Beckman, DL; Goldman, BS; Kranz, RG; Monika, EM | 1 |
| Baralle, FE; Garcia, R; Pagani, F; Pariyarath, R; Stuani, C | 1 |
| Henriksen, CM; Kristiansen, KN; Nielsen, J; Theilgaard, HB | 1 |
| Ciucci, A; Manzini, S; Palma, C; Riitano, D; Werge, TM | 1 |
| LeMaster, DM; Springer, PA; Unkefer, CJ | 1 |
| Dunbar, SA; Winkler, HH | 1 |
| Berry, MJ; Harney, JW; Larsen, PR; Sun, BC | 1 |
| Chen, ZH; Gopalakrishna, R; Gundimeda, U | 1 |
| Hediger, MA; Nussberger, S; Trotti, D; Volterra, A | 1 |
| Eu, JP; Meissner, G; Stamler, JS; Xu, L | 1 |
| Holmgren, A; Nikitovic, D; Spyrou, G | 1 |
| Aust, SD; Spear, N | 1 |
| Banditelli, S; Cappiello, M; Cecconi, I; Dal Monte, M; Del Corso, A; Moschini, R; Mura, U; Tsai, L; Vilardo, PG | 1 |
| Cermák, J; Kárová, B; Krepela, E; Procházka, J; Roubková, H | 1 |
| Baines, IC; Kang, SW; Rhee, SG | 1 |
| Iwaoka, M; Juminaga, D; Scheraga, HA | 1 |
| Edmiston, PL; Saavedra, SS | 1 |
| Lauren, SL; Treuheit, MJ | 1 |
| Dixon, JE; Zhuo, S | 1 |
| Becker, K; Karplus, PA; Keese, M; Savvides, SN; Schirmer, RH | 1 |
| Bosoni, T; Montalbetti, L; Moratti, R; Rizzo, V; Scoglio, E; Valli, M | 1 |
| Katagiri, Y; Nakamura, M; Nakashima, S; Nozawa, Y | 1 |
| Biocca, S; Cattaneo, A; Pierandrei-Amaldi, P; Ruberti, F; Tafani, M | 1 |
| Beber, FA; Brigo, MJ; Pereira, CN; Rocha, JB; Silva, MC; Wollmeister, J | 1 |
| Mulrooney, SB; Veine, DM; Wang, PF; Williams, CH | 1 |
| Kitazume-Kawaguchi, S; Sevigny, MB; Troy, FA; Ye, J | 1 |
| De Caro, A; De Caro, J; Verger, R | 1 |
| Gooley, AA; Herbert, BR; Kett, WC; Packer, NH; Williams, KL; Yan, JX | 1 |
| Cusick, CL; Freeman, ML; Lu, S; Meredith, MJ; Sekhar, KS; Soltaninassab, S | 1 |
| Lennarz, WJ; Luz, JM | 1 |
| Bottje, WG; Cawthon, D; Wang, S | 1 |
| Figliomeni, S; Liu, SM | 1 |
| Kim, HG; Lim, CJ; Park, EH | 1 |
| Kudla, J; Liang, S; Luan, S; Xu, Q | 1 |
| Lynch, JW | 1 |
| Katayama, H; Miyakoshi, M; Murayama, T; Nomura, Y; Yamada, T | 1 |
| Radi, R; Souza, JM | 1 |
| Fakler, B; Hahn, H; Ruppersberg, JP; Schulte, U; Wiesinger, H | 1 |
| Cho, YW; Han, TJ; Jin, CD; Kim, JC; Lim, CJ | 1 |
| Demple, B; Ding, H | 1 |
| Briddon, A | 1 |
| Chen, C; Liu-Chen, LY; Shahabi, V; Xu, W | 1 |
| Davis, DA; Gillim, LA; Mitsuya, H; Newcomb, FM; Yarchoan, R; Yusa, K | 1 |
| Lundell, N; Schreitmüller, T | 1 |
| Swiedler, SJ; Wei, Z | 1 |
| Apostol, I; Lippincott, J | 1 |
| Griffiths, G; Locker, JK | 1 |
| Ito, K; Kobayashi, T | 1 |
| Murayama, T; Naganuma, T; Nomura, Y | 1 |
| Bertollo, DM; Moreira, CM; Oliveira, EM; Vassallo, DV; Veloso, TC | 1 |
| Ballicora, MA; Frueauf, JB; Fu, Y; Preiss, J | 1 |
| Gold, MH; Reddy, GV | 1 |
| Kozaki, A; Sasaki, Y | 1 |
| Tang, B; Wei, C; Yang, K; Zhang, Y | 1 |
| Harris, CR; Silhavy, TJ | 1 |
| Broekelmann, T; Mecham, RP; Ritty, TM; Tisdale, C; Trask, TM | 1 |
| Hisabori, T; Motohashi, K; Stumpp, MT | 1 |
| Hata, F; Ishii, T; Nakajima, H; Nishio, H; Sunami, O; Takeuchi, T | 1 |
| Metodiewa, D; Winterbourn, CC | 1 |
| Lee, SH; Park, JS; Park, SJ; Wang, M | 1 |
| Miller, DA; Suo, Z; Walsh, CT | 1 |
| Abraham, RT; Choi, M; Reiter, TA; Rusnak, F | 1 |
| Elbetieha, A; Hussein, E; Owais, WM; Saadoun, I | 1 |
| Capellari, S; Perry, G; Petersen, RB; Smith, MA; Urig, CB; Zaidi, SI | 1 |
| Brett, SE; Leary, SC; Leatherland, JF; Welsh, DG | 1 |
| Dulhunty, AF; Fink, RH; Haarmann, CS | 1 |
| Martínez-Chantar, ML; Pajares, MA | 1 |
| Nicoll, DA; Ottolia, M; Philipson, KD; Santacruz-Toloza, L | 1 |
| Arana, ME; Boehmer, PE; Sampson, DA | 1 |
| Asai, Y; Homma, M; Kawagishi, I; Shoji, T | 1 |
| Jiang, Sq; Paulus, H; Shingledecker, K | 1 |
| Liu, X; Wei, M; Wu, D; Yang, X | 1 |
| Lenzen, S; Richter, T; Tiedge, M | 1 |
| Johannes, C; Majcherczyk, A | 1 |
| Moulin, A; Puigserver, A; Smialowski-Fléter, S; Villard, C | 1 |
| Backx, PH; Cho, HC; Guy, HR; Nguyen, TT; Tsushima, RG | 1 |
| Cross, CE; Jones, AD; Reddy, S; Van Der Vliet, A; Wong, PS | 1 |
| Chen, X; Liu, Z; Wang, K; Wang, PG; Xian, M | 1 |
| Clarke, DM; Loo, TW | 2 |
| Berthelier, V; Boulla, G; Deterre, P; Laboureau, J; Schuber, F | 1 |
| Bedows, E; Darling, RJ; Perini, F; Ruddon, RW | 1 |
| Hoyer, S; Yun, SW | 1 |
| Chai, CL; Hurne, AM; Waring, P | 1 |
| Harvey, JR; Klemm, MF; Lang, RJ; McPhee, GJ | 1 |
| Gotoh, T; Kikuchi, K | 1 |
| Lipton, SA; Pan, ZH; Zhang, X | 1 |
| Alliegro, MC | 1 |
| Billiar, TR; Seol, DW | 1 |
| Arras, T; Becker, S; Holighaus, G; Ohlberger, G; Tran, QH; Unden, G | 1 |
| Hook, VY; Hwang, SR; Steineckert, B | 1 |
| Cordeiro, MN; Diniz, CR; Gomez, MV; Kalapothakis, E; Prado, MA; Reis, HJ; Romano-Silva, MA | 1 |
| Cha, MK; Kim, IH; Lee, H | 1 |
| Hayakari, M; Kimura, J; Kumano, T; Sawamura, D; Tsuchida, S; Yamazaki, T | 1 |
| Azza, S; Boschi-Muller, S; Branlant, G; Sanglier-Cianferani, S; Talfournier, F; Van Dorsselear, A | 1 |
| Hisatome, I; Igawa, O; Kitamura, F; Kurata, Y; Makita, N; Miake, J; Morisaki, H; Morisaki, T; Ogino, K; Sasaki, N; Sato, R; Shigemasa, C; Takeda, Si; Tanaka, Y; Taniguchi, Si; Tsuboi, M; Urashima, T; Yatsuhashi, T; Yoshida, A | 1 |
| Blank, VC; Peña, C; Roguin, LP | 1 |
| Patston, PA; Simonovic, I | 1 |
| Hara, N; Shimoyama, M; Terashima, M; Tsuchiya, M | 1 |
| Baganoff, M; Bruce, C; Connolly, DT; Duffin, K; Glenn, K; Hegde, S; Heuvelman, D; Hope, HR; Krul, E; Lee, L; Schilling, R; Smith, C; Tall, AR; Witherbee, B; Zablocki, J | 1 |
| Cho, H; Choi, MU; Kim, JH; Ryu, SE | 1 |
| Feng, YH; Karnik, SS; Saad, Y | 1 |
| Hunter, CN; Jensen, PE; Reid, JD | 1 |
| Berry, RW; Binder, LI; Gamblin, TC; King, ME; Kuret, J | 1 |
| Bedows, E; Darling, RJ; Ruddon, RW; Wilken, JA | 1 |
| Sissons, CH; Yakub, S | 1 |
| Chen, HV; Choi, Y; Lipton, SA | 1 |
| Garrison, G; George, GN; Heaton, DN; Winge, DR | 1 |
| Becker, K; Rahlfs, S | 1 |
| Dulhunty, A; Green, D; Haarmann, C; Hart, J | 1 |
| Chhajlani, V; Doufexis, M; Frändberg, PA; Kapas, S | 1 |
| Fontecave, M; Mulliez, E; Padovani, D | 1 |
| Gilbert, HF; Zheng, J | 1 |
| Haddrill, J; Han, NL; Lynch, JW; Pierce, KD; Schofield, PR | 1 |
| Cai, K; Itoh, Y; Khorana, HG | 1 |
| Kim, IG; Oh, TJ; Park, SY | 1 |
| Di Stasi, MA; Mallozzi, C; Minetti, M | 1 |
| Gupta, N; Porter, TD | 1 |
| Bhattacharya, K; Evans, DB; Rank, KB; Sharma, SK | 1 |
| Dieguez-Acuña, FJ; Ellis, ME; Kushleika, J; Woods, JS | 1 |
| Geczy, CL; Raftery, MJ; Valenzuela, SM; Yang, Z | 1 |
| Chaudhuri, AR; Khan, IA; Ludueña, RF | 1 |
| Smith, JW; Yan, B | 1 |
| Jevtovic-Todorovic, V; Mennerick, S; Meyenburg, A; Olney, JW; Perez-Reyes, E; Romano, C; Todorovic, SM; Zorumski, CF | 1 |
| Kozaki, A; Mayumi, K; Sasaki, Y | 1 |
| Kelley, MR; Parsons, SH | 1 |
| Kitabatake, N; Wada, R | 1 |
| Spies, HS; Steenkamp, DJ; Vogt, RN | 1 |
| Han, Y; Li, X; Pan, XM | 1 |
| Alessio, M; Arese, P; Bussolino, F; Ferrandi, C; Gruarin, P; Primo, L; Tandon, NN; Ulliers, D | 1 |
| Beniash, E; Hartgerink, JD; Stupp, SI | 1 |
| Bendahan, A; Brocke, L; Grunewald, M; Kanner, BI | 1 |
| Byers, HL; Eaton, P; Leeds, N; Shattock, MJ; Ward, MA | 1 |
| Ayene, IS; Biaglow, JE; Jenkins, SF; Koch, CJ; Mauldin, SK; Stamato, TD; Tuttle, SW | 1 |
| Ennion, SJ; Evans, RJ | 1 |
| Kaplowitz, N; Mittur, A; Wolkoff, AW | 1 |
| Gordienko, DV; Greenwood, IA; Large, WA; Leblanc, N | 1 |
| González, C; Latorre, R; Lissi, E; Soto, MA; Vergara, C | 1 |
| Klootwijk, W; Kuiper, GG; Visser, TJ | 1 |
| Clyne, JD; Hume, RI; Wang, LF | 1 |
| Rosenthal, PJ; Shenai, BR | 1 |
| Chauhan, R; Mande, SC | 1 |
| Hikasa, Y; Inaba, M; Matsuki, N; Ono, K; Sato, K; Tamahara, S | 1 |
| Pitts, KE; Summers, AO | 1 |
| Dubocovich, MI; Gerdin, MJ; Mseeh, F | 1 |
| Musib, R; Phillips, M; Reisler, E; Rubenstein, PA; Shvetsov, A | 1 |
| Beckman, DL; Enright, E; Liu, L; Mehta, P; Sun, P; Terrian, DM; Tsai, SY | 1 |
| Haddrill, JL; Lynch, JW; Shan, Q | 1 |
| Jeong, HS; Jeong, IC; Kang, HS; Kang, SW; Kim, A; Kim, YJ; Lee, SH; Park, JS | 1 |
| Leiper, J; McDonald, N; Murray-Rust, J; Vallance, P | 1 |
| Emi, Y; Ikushiro, S; Iyanagi, T | 1 |
| Binet, MB; Corker, H; Moir, AJ; Pittman, MS; Poole, RK; Wu, G | 1 |
| Jiang, M; Liu, J; Tseng, GN; Zhang, M | 1 |
| Demasi, M; Netto, LE; Silva, GM | 1 |
| Daou, MC; Jones, SN; Ross, AH; Schiffer, CA; Schonhoff, CM | 1 |
| Hatters, DM; Howlett, GJ; Lawrence, LJ; Pham, CL | 1 |
| Angelisova, P; Boyson, JE; Chiu, M; Erskine, R; Horejsi, V; Koopman, LA; Lau, JM; Strominger, JL; Valter, MM; Whitman, MC | 1 |
| Hayward, LJ; Tiwari, A | 1 |
| Braakman, I; Jansens, A; van Duijn, E | 1 |
| Berg, AH; Brownlee, M; Combs, TP; Du, X; Engel, J; Pajvani, UB; Rajala, MW; Scherer, PE; Schulthess, T | 1 |
| Calvete, JJ; Gasset, M; Pajares, MA; Sanchez-Perez, GF | 1 |
| Rozanski, GJ; Xu, Z | 1 |
| Gross, AK; Oprian, DD; Xie, G | 1 |
| Patton, AK; Stenson, TH; Weiss, AA | 1 |
| Anishkin, A; Chiang, CS; Gendel, V; Guy, HR; Sharifi, NA; Shirinian, L; Sukharev, S | 1 |
| Chollet, J; Gao, HF; Jiao, PY; Mei, JY; Tanner, M; Utzinger, J; Xiao, SH; You, JQ | 1 |
| Donovan, CM; Flagel, LE; Olhoft, PM; Somers, DA | 1 |
| Hultberg, B | 1 |
| Ishikawa, K; Mino, K | 1 |
| Jackson, W; Kumar, KS; Seed, TM; Singh, VK | 1 |
| Alfonso-Rubí, J; Armas, R; Castañera, P; Coll, Y; González, A; Hernández, CA; Ortego, F; Pérez, M; Pujol, M; Ruiz, M | 1 |
| Akimoto, S; Nakamura, H; Nakamura, K; Shiro, Y; Tanaka, A | 1 |
| Dailey, HA; Dailey, TA; Rose, JP; Wang, BC; Wu, CK | 1 |
| Ito, S; Koda, N; Niwa, H; Ohkubo, H; Ohmiya, Y; Tanikawa, N; Watanabe, K | 1 |
| Kristl, A; Legen, I | 1 |
| Ascenzi, P; Bolognesi, M; Bordo, D; Forlani, F; Pagani, S; Salvati, L; Spallarossa, A; Visca, P | 1 |
| Ding, K; Li, F; Sun, H; Yan, S | 1 |
| Chu, F; O'Brian, CA; Stewart, JR; Ward, NE | 1 |
| Koizumi, F; Matsuda, Y; Nakanishi, S | 1 |
| Allen, S; Lu, H; Thornton, D; Tokatlidis, K | 1 |
| Kots, A; Martin, E; Murad, F; Sharina, I | 1 |
| Gellon, JO; Girault, HH; Jensen, H; Lion, N | 1 |
| Bayley, H; Luchian, T; Shin, SH | 1 |
| Bryson, S; Chang, X; Datti, A; Lew, AM; Macher, BA; Takeshita, S; Tse, R; Tvaroska, I; Yen, TY | 1 |
| Borges, VC; Nogueira, CW; Rocha, JB; Zeni, G | 1 |
| Heuser, JE; Hug, C; Lee, DH; Lodish, HF; Murrey, HE; Ruderman, NB; Tomas, E; Tsao, TS | 1 |
| Allen, JW; Barker, PD; Ferguson, SJ | 1 |
| Barŕon, L; Michaelis, EK; Michaelis, ML; Schöneich, C; Sharov, VS; Zaidi, A | 1 |
| Hess, S; Pikis, A; Thompson, J | 1 |
| Grazziani, S; Gutova, M; Hamann, M; Han, S; Makmura, L; Momand, J; Nguyen, J; Sheppard, S; Sun, XZ; Thomas, J; Tran, D; Vinci, C; Zhou, F | 1 |
| Budde, AD; Jones, BL | 1 |
| Clark, E; Gandhi, CS; Isacoff, EY; Loots, E; Pralle, A | 1 |
| Caplan, JF; Filipenko, NR; Fitzpatrick, SL; Waisman, DM | 1 |
| Atmane, N; Dairou, J; Dupret, JM; Rodrigues-Lima, F | 1 |
| Graf, PC; Hoffmann, JH; Jakob, U; Lilie, H; Linke, K | 1 |
| Steenkamp, DJ; Vogt, RN | 1 |
| Ambahera, S; Beck, JL; de Jersey, J; Ralph, SF; Sheil, MM; Yong, SR | 1 |
| Sobolevsky, AI; Wollmuth, LP; Yelshansky, MV | 1 |
| Koreeda, H; Yamaguchi, T | 1 |
| Chevallet, M; Diemer, H; Leize-Wagner, E; Luche, S; Rabilloud, T; Tastet, C; Van Dorsselaer, A | 1 |
| Nogueira, CW; Rocha, JB; Santos, FW; Soares, FA | 1 |
| Cheng, Y; Hui, EF; Pan, JC; Zhou, HM | 1 |
| Bateman, KP; Dubé, D; Friesen, RW; Gresser, MJ; Huang, Z; LeRiche, TG; Pollex, R; Ramachandran, C; Sanghara, J; Trimble, L; Wang, Q | 1 |
| Bai, R; Copeland, TD; Covell, DG; Hamel, E; Kepler, JA; Nguyen, NY; Pettit, GR; Taylor, GF | 1 |
| Cross, JV; Templeton, DJ | 1 |
| Nomura, Y; Ogino, S; Tsuruma, K; Uehara, T | 1 |
| Biniossek, ML; Darie, CC; Jovine, L; Litscher, ES; Wassarman, PM | 1 |
| Gragen, S; Li, Y; Liu, J; Ma, Y; Matoney, L; Song, X; Yan, B; Yang, D | 1 |
| Benison, GC; Cosper, NJ; Di Lello, P; Legault, P; Omichinski, JG; Scott, RA; Shokes, JE | 1 |
| Amy Sang, QX; Kang, T; Tschesche, H | 1 |
| den Hertog, J; Overvoorde, J; van der Wijk, T | 1 |
| Leelavanichkul, K; Pitt, BR; St Croix, CM; Stitt, MS; Wasserloos, KJ; Watkins, SC | 1 |
| Esposito, EA; Martin, CT | 1 |
| Behshad, E; Bollinger, JM; Parkin, SE | 1 |
| Brinkmann, T; Götting, C; Kleesiek, K; Kuhn, J; Müller, S; Prante, C; Schön, S; Schöttler, M | 1 |
| Hayashi, S; Higashimoto, Y; Noguchi, M; Omata, Y; Sakamoto, H | 1 |
| Lockwood, TD | 2 |
| Akabas, MH; Horenstein, J; Riegelhaupt, P | 1 |
| Bontempo, D; Fish, BA; Heredia, KL; Maynard, HD | 1 |
| Eapen, A; Sharma, A; Subbarao, SK | 1 |
| Ens, W; Giorgianni, A; Hasinoff, BB; Jiang, Y; Krokhin, OV; Nitiss, JL; Sivaram, T; Standing, KG; Wu, X; Yalowich, JC; Yang, S | 1 |
| Kashlan, OB; Kleyman, TR; Perry, CJ; Sheng, S | 1 |
| Gautschi, I; Kellenberger, S; Pfister, Y; Schild, L | 1 |
| Bai, G; Jin, Y; Liu, Z; Yang, W | 1 |
| Adachi, T; Brenner, DA; Cohen, RA; Colucci, WS; Ido, Y; Kuster, GM; Liao, R; Pimentel, DR; Siwik, DA | 1 |
| Hirabayashi, T; Murayama, T; Nabemoto, M; Nakamura, H; Nomura, Y; Ohsawa, K; Okuma, Y; Saito, T | 1 |
| Gee, CL; Grunewald, GL; Hsin, AY; Martin, JL; McLeish, MJ; Nourse, A; Tyndall, JD; Wu, Q | 1 |
| Dellal, SS; Hume, RI; Nagaya, N; Saar, N; Tittle, RK | 1 |
| Aracena, P; Faúndez, M; Lepe, AM; Letelier, ME; Pimentel, A; Pino, P; Speisky, H | 1 |
| Inaba, K; Ito, K; Takahashi, YH | 1 |
| Feng, Z; Qin, G; Xicheng, W | 1 |
| Akabas, MH; Bera, AK | 1 |
| Aposhian, HV; Chowdhury, UK; Hernandez, A; Tsaprailis, G; Zakharyan, RA | 1 |
| Katayama, A; Nagahara, N | 1 |
| Cumming, RC; Schubert, D | 1 |
| Hori, S; Kakizuka, A; Kimura, Y; Koike, M; Manno, A; Murakami, K; Noguchi, M; Ohizumi, H; Takata, T | 1 |
| Benz, FW; Cai, J; Feng, W; Kang, YJ; Pierce, WM | 1 |
| Gladyshev, VN; Kim, HY | 1 |
| Chen, HZ; Chen, JS; Liang, XX; Qiu, PX; Sun, HH; Wu, YP; Yan, GM; Zhou, YN | 1 |
| Andrews, D; Davidson, D; Hill, BC; Imriskova-Sosova, I; Yachnin, B; Yam, K | 1 |
| Clark, DD; Ensign, SA; Nocek, B; Pandey, AS; Peters, JW | 1 |
| Chait, BT; Fushitani, K; Gorr, TA; Kao, WY; Knapp, JE; Qin, J; Riggs, AF; Riggs, CK; Smith, SS | 1 |
| Alexander, C; Borg, E; Dawson, DC; Liu, X; Serrano, J | 1 |
| Bissonnette, P; Gagnon, DG; Lapointe, JY | 1 |
| Blakytny, R; Brunner, G; Erkell, LJ | 1 |
| Chang, TS; Jeong, W; Lee, DY; Park, SJ; Rhee, SG | 1 |
| Bao, S; Ramanadham, S; Song, H; Turk, J | 1 |
| Chang, JY; Lin, CC; Lu, BY | 1 |
| Rudolph, J; Seth, D | 1 |
| Gupta, N; Pop, SM; Ragsdale, SW; Raza, AS | 1 |
| Barić, I; Beluzić, R; Cuk, M; Fumić, K; Jurak, I; Mudd, SH; Pavkov, T; Vugrek, O | 1 |
| Doig, AJ; Errington, N; Iqbalsyah, TM; Moutevelis, E; Warwicker, J | 1 |
| Jacquot, JP; Krimm, I; Lancelin, JM; Lemoine, J; Noguera-Mazon, V; Rouhier, N; Salvador, A; Walker, O | 1 |
| Cao, L; DiRienzo, JM; Korostoff, J; Volgina, A | 1 |
| Arisaka, F; Fusada, N; Hara, S; Hisabori, T; Hosoya-Matsuda, N; Kikuchi, N; Motohashi, K; Romano, PG | 1 |
| Aviram, M; Rozenberg, O | 1 |
| Eldakak, A; Hulett, FM | 1 |
| Amazonas, JN; Batista, EJ; De Menezes, LF; de Sá Pinheiro, AA; de Souza Barros, F; De Souza, W; Meyer-Fernandes, JR; Silva, EF | 1 |
| Abe, Y; Matsumura, T; Nagahara, N; Yoshii, T | 1 |
| Chen, L; Chen, R; Gu, HH; Han, DD; Hill, ER; Jiang, L; Wei, H | 1 |
| Cravatt, BF; Dubin, AE; Evans, MJ; Macpherson, LJ; Marr, F; Patapoutian, A; Schultz, PG | 1 |
| Hansen, RE; Nørgaard, P; Winther, JR; Østergaard, H | 1 |
| Bhargava, Y; Eisele, T; Marquez-Klaka, B; Nicke, A; Rettinger, J | 1 |
| Luo, A; Ma, J; Wang, W; Zhang, P; Zhou, H | 1 |
| Ayoub, MA; Berque-Bestel, I; Berthouze, M; Fischmeister, R; Jockers, R; Lezoualc'h, F; Lucas, A; Rivail, L; Russo, O; Sicsic, S | 1 |
| Duban, ME; Janik, D; Joachimiak, A; Kern, JW; Maresso, AW; Missiakas, DM; Schneewind, O; Wu, R; Zhang, R | 1 |
| Lee, JK; Plemper, RK; Prussia, A; Snyder, JP | 1 |
| Chen, M; Harmon, CM; Kesterson, RA; Yang, Y | 1 |
| Betté, S; Castagna, M; Mandela, PG; Mari, SA; Peres, A; Rudnick, G; Sacchi, VF; Santacroce, M; Soragna, A | 1 |
| Beavers, MP; Chiu, CH; Diamond, SL; Huryn, DM; Motlekar, N; Myers, MC; Napper, AD; Shah, PP; Smith, AB | 1 |
| Meng, FG; Mu, H; Park, YD; Yang, JM; Zhou, SM | 1 |
| Arshavsky, VY; Bowes Rickman, C; Ebright, JN; Kelly, U; Malek, G; McKay, BS; Rickman, DW; Saloupis, P; Yu, L | 1 |
| Li, M; Wang, XY; Zhu, WJ | 1 |
| Liu, X | 1 |
| Doe, I; Kawabata, A; Kubo, S; Kurokawa, Y; Masuko, T; Sekiguchi, F | 1 |
| Au, KW; Lau, CP; Li, RA; Siu, CW; Tse, HF | 1 |
| Buckley, BJ; Li, S; Whorton, AR | 1 |
| Ohisa, N; Ohno, T; Toeda, K; Tomatsu, M | 1 |
| Daiber, A; Kawamoto, T; Kleinert, H; Müller, J; Münzel, T; Oelze, M; Pautz, A; Schuhmacher, S; Schulz, E; Wenzel, P; Wojnowski, L; Zurmeyer, S | 1 |
| Brandstätter, L; Diederichs, K; Eicher, T; Pos, KM; Seeger, MA; Verrey, F; von Ballmoos, C | 1 |
| Hsieh, CP | 1 |
| Kang, HS; Kim, CH; Kim, DK; Kim, HE; Kim, S; Kim, SS; Kwak, IJ; Lee, YJ; Li, CR; Lu, M; Moon, JY; Park, JS | 1 |
| Corbier, C; Didierjean, C; Gelhaye, E; Hirasawa, M; Jacquot, JP; Knaff, DB; Koh, CS; Navrot, N; Rouhier, N; Samian, R; Wingsle, G | 1 |
| Chen, JG; Fu, H; Hu, ZL; Liu, J; Liu, RL; Long, LH; Wang, F; Xie, N | 1 |
| Dokholyan, NV; Goda, K; Hegedus, C; Hegedus, T; Laczkó, R; Litman, T; Ozvegy-Laczka, C; Sarkadi, B; Sorrentino, BP; Váradi, A; Várady, G | 1 |
| Cho, S; Dreyfuss, G; Ottinger, E; Wan, L | 1 |
| Ebrahim-Habibi, A; Nemat-Gorgani, M; Ramshini, H; Rezaei-Ghaleh, N; Saboury, AA | 1 |
| Kim, HY; Lee, TH | 1 |
| Beretta, M; Gorren, AC; Mayer, B; Schmidt, K; Sottler, A | 1 |
| Dos Santos, DB; Nogueira, CW; Paixão, MW; Rocha, JB; Schiar, VP; Zeni, G | 1 |
| Bayley, H; Chen, M; Li, QH | 1 |
| Brown, S; Sanguinetti, MC; Sonntag, DP | 1 |
| Bankston, JR; Chan, PJ; Chung, DY; Karlin, A; Kass, RS; Liu, G; Marx, SO; Yang, L | 1 |
| Buck, F; Hilz, H; Meyer, A; Weber, W | 1 |
| Chen, CY; Rudolph, J; Willard, D | 1 |
| Andries, M; Bollen, M; Derua, R; Jansen, S; Waelkens, E | 1 |
| Altenberg, GA; Retamal, MA; Reuss, L; Yin, S | 1 |
| Au, KW; Azene, EM; Lau, CP; Li, RA; Siu, CW; Tse, HF | 1 |
| Dobson, RL; Foertsch, LM; Gerberick, GF; Goebel, C; Lepoittevin, JP; Quijano, M; Troutman, JA; Vassallo, JD | 1 |
| Liu, YZ; Luo, ZF; Peng, LL; Shen, DK; Wu, H; Xu, XL; Zhang, LY | 1 |
| Crisman, TJ; Forrest, LR; Kanner, BI; Qu, S | 1 |
| Cruchaga, C; Gitcho, MA; Goate, A; Robberecht, W; Seijo-Martínez, M; Van Damme, P; Vidal, JM; Wang, J; Wang, L; Wu, JY | 1 |
| Dwivedi, N; Sau, AK; Srivastava, A | 1 |
| Aniya, Y; Nakama, S; Oshiro, N | 1 |
| Bossy, B; Bossy-Wetzel, E; Chen, J; Klinglmayr, E; Knott, AB; Lütz-Meindl, U; Masliah, E; Petrilli, A; Schwarzenbacher, R | 1 |
| Feustel, PJ; Jourd'heuil, D; Mongin, AA; Rudkouskaya, A; Shah, AA; Sim, V | 1 |
| Beyl, S; Hering, S; Karmazinova, M; Klugbauer, N; Lacinova, L; Stary-Weinzinger, A; Suwattanasophon, C | 1 |
| Anwer, MS; Ramasamy, U; Schonhoff, CM | 1 |
| de Sousa, VP; Pinto, JR; Sorenson, MM | 1 |
| Nimura, N; Shingaki, T | 1 |
| Chernorizov, KA; Elkina, JL; Muronetz, VI; Semenyuk, PI; Svedas, VK | 1 |
| Fujiki, Y; Matsumoto, Y; Misono, S; Miyata, N; Mukai, S; Okumoto, K | 1 |
| Neubauer, P; Siurkus, J | 1 |
| Carr, CM; O'Cualain, RD; Sims, PF | 1 |
| Dicesare, JC; Kennedy, S; Sheaff, RJ | 1 |
| Aleanzi, MC; Bosco, MB; Iglesias, AÁ | 1 |
| Chung, HY; Jeong, HO; Li, C; Liu, XX; Park, D; Sun, S; Zhou, HM | 1 |
| Alhamidi, M; Brox, V; Fagerheim, T; Kjeldsen Buvang, E; Lindal, S; Nilssen, Ø; Van Ghelue, M | 1 |
| Benner, J; Bollinger, JM; Considine, N; O'Neill, C; Perler, FB; Saleh, L; Southworth, MW | 1 |
| Kazi, R; Talukder, I; Wollmuth, LP | 1 |
| Bezbradica, D; Bolivar, JM; de las Rivas, B; Fernandez-Lorente, G; Godoy, CA; Guisan, JM; López-Gallego, F | 1 |
| Kot, M; Olech, Z | 1 |
| Booz, GW; Duhé, RJ; Gunter, BW; Patil, CN; Patlolla, S; Smith, JK | 1 |
| Lu, L; Shimizu, T; Tanaka, K; Yokotani, K | 1 |
| Fragoulis, EG; Kiritsi, MN; Sideris, DC | 1 |
| Glaring, MA; Issakidis-Bourguet, E; Moorhead, GB; Schriemer, DC; Silva, LP; Silver, DM | 1 |
| Cosentino-Gomes, D; Meyer-Fernandes, JR; Paletta-Silva, R; Vieira-Bernardo, R | 1 |
| Chen, L; Han, Y; Huang, C; Jia, H; Jia, W; Li, B; Liang, L; Ren, H; Sun, M; Wang, Y; Wu, Z; Xing, Y; Zhao, Y | 1 |
| Fu, B; Jiang, Q; Kapadia, MR; Kibbe, MR; Martinez, J; Tsihlis, ND; Vavra, AK | 1 |
| Althaus, M; Alvarez de la Rosa, D; Clauss, WG; Fronius, M; Giraldez, T; Krumm, P | 1 |
| Kil, IS; Park, JW; Shin, SW | 1 |
| Anderson, CM; Choi, YE; Eskandari, S; Maestas, MJ; Omoto, JJ; Rahnama-Vaghef, A; Salto, G; Sanchez, RV | 1 |
| Aseeva, E; Bonardi, V; Leister, D; Liu, Q; Pribil, M; Wunder, T | 1 |
| Forster, MJ; Sumien, N; Thangthaeng, N; Yan, LJ | 1 |
| Liu, X; Subramani, S | 1 |
| Baek, K; Lee, JG; Soetandyo, N; Ye, Y | 1 |
| Adámik, M; Brázdová, M; Fojta, M; Navrátilová, L; Tichý, V | 1 |
| Groeneweg, S; Lima de Souza, EC; Peeters, RP; Visser, TJ; Visser, WE | 1 |
| Jiang, W; Mao, L; Wang, D; Yang, L; Yu, P; Zhuang, X | 1 |
| Kumeta, M; Otsuka, S; Taga, M; Takeyasu, K; Yoshimura, SH | 1 |
| Cernocká, H; Ostatná, V; Paleček, E | 1 |
| Bechtold, E; Furdui, CM; King, SB; Poole, LB; Reisz, JA | 1 |
| Brodbelt, JS; Browning, KS; Mayberry, LK; Murphy, PA; O'Brien, JP | 1 |
| Farrens, DL; Fay, JF | 1 |
| Bermejo-Barrera, P; Domínguez-González, R; González Varela, L | 1 |
| Qu, S; Zhang, X; Zhang, Y | 1 |
| Dyrlund, TF; Enghild, JJ; Nielsen, NC; Nielsen, TA; Poulsen, ET; Risør, MW; Sanggaard, KW; Thomsen, LR | 1 |
| Gupta, N; Puri, N; Saini, S; Sharma, P; Sondhi, S | 1 |
| Bedhomme, M; Calvaresi, M; Fermani, S; Lemaire, SD; Marchand, CH; Michelet, L; Morisse, S; Trost, P; Zaffagnini, M | 1 |
| Dong, S; Gai, H; Sun, L; Wang, Q; Zhao, W | 1 |
| Adamus, J; Dębski, D; Kalyanaraman, B; Marcinek, A; Michałowski, B; Sikora, A; Smulik, R; Zielonka, J | 1 |
| Chen, HY; Li, Y; Xu, JJ; Yu, XD; Zheng, LQ | 1 |
| Brandl, CJ; Dubinsky, M; Gyenis, L; Innes, BT; Konermann, L; Litchfield, DW; Shilton, BH; Sowole, MA | 1 |
| Kaltashov, IA; Wang, S | 1 |
| Eger, BT; Kawaguchi, Y; Matsumura, T; Nishino, T; Okamoto, K; Pai, EF | 1 |
| Alam, S; Dierks, T; Mariappan, M; May, C; Peng, J; Radhakrishnan, K; Rudolph, MG; Schmidt, B; von Figura, K | 1 |
| Rogowska-Wrzesinska, A; Wojdyla, K | 1 |
| Bariola, PA; Grabstein, K; Graddis, TJ; Li, G; Nairn, NW; VanBrunt, MP; Wang, A | 1 |
| Hofele, R; Kohlbacher, O; Kramer, K; Lenz, C; Richter, FM; Sachsenberg, T; Urlaub, H; Zaman, U | 1 |
| Cserne Szappanos, H; Hool, L; Ingley, E; Muralidharan, P | 1 |
| Eastland, A; Hornick, J; Kawamura, R; Marko, JF; Nanavati, D | 1 |
| Cai, R; Chen, C; Grewer, C; Guo, Z; Wang, J; Yuan, Y; Zhang, Z | 1 |
| Giordano, M; Gontero, B; Lebrun, R; Prioretti, L | 1 |
| Chadalawada, S; Khandelwal, R; Sharma, AK; Sharma, Y | 1 |
| Grant, GA | 1 |
| Chen, H; Li, C; Liu, Y; Ma, Z; Mo, Z; Su, Z; Tan, L; Xie, J; Xu, Y; Yang, X; Zhou, J | 1 |
| Ebihara, Y; Kawakami, Y; Yamauchi, K | 1 |
| Alsudir, S; Lai, EPC | 1 |
| Müller, T; Winter, D | 1 |
| Couturier, J; Keech, O; Rouhier, N; Zannini, F | 1 |
| Akaike, T; Bogdándi, V; Dick, TP; Dustin, CM; Fukuto, JM; Heppner, DE; Hristova, M; Ida, T; Li, J; Mijuskovic, A; Nagy, P; van der Vliet, A | 1 |
| Mukhtasimova, N; Sine, SM | 1 |
| Cho, HJ; Cho, HY; Huh, TL; Kang, BS; Kwon, OS; Lee, HS; Park, JW | 1 |
| He, S; Qu, S; Zhang, W; Zhang, X | 1 |
| Endow, JK; Fukushima, J; Inoue, K; McKinnon, LJ; Theg, SM | 1 |
| Himeno, S; Ogawa, M; Okamoto, Y; Sumi, D; Suzukawa, K | 1 |
| Arvizu-Flores, AA; Cruz-Valencia, R; Rosas-Rodríguez, JA; Valenzuela-Soto, EM | 1 |
| Lees, WJ; Patel, AS | 1 |
| Demir, D; Gençer, N; Kucukislamoglu, M; Sonmez, F | 1 |
5 review(s) available for cysteine and dithiothreitol
| Article | Year |
|---|---|
Effect of vitamin C on tubulin tyrosinolation in polymorphonuclear leukocytes.
Topics: Ascorbic Acid; Chediak-Higashi Syndrome; Cysteine; Dithiothreitol; Glutathione; Humans; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Oxidation-Reduction; Protein Processing, Post-Translational; Tubulin; Tyrosine | 1987 |
Pyrene excimer fluorescence as a probe of protein conformational change.
Topics: Animals; Cysteine; Dithiothreitol; Histones; Kinetics; Protein Conformation; Protein Structure, Secondary; Proteins; Pyrenes; Spectrometry, Fluorescence; Tropomyosin; Troponin; Troponin C; Troponin I | 1995 |
How many cysteine residues regulate ryanodine receptor channel activity?
Topics: Animals; Calcium Signaling; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione; Heart; Ion Transport; Muscle Contraction; Muscle Proteins; Muscle, Skeletal; Myocardial Contraction; Myocardium; Oxidants; Oxidation-Reduction; Protein Conformation; Protein Structure, Tertiary; Protein Subunits; Pyridines; Reducing Agents; Ryanodine Receptor Calcium Release Channel; Sarcoplasmic Reticulum; Structure-Activity Relationship; Sulfhydryl Compounds; Thimerosal | 2000 |
Differential alkylation-based redox proteomics--Lessons learnt.
Topics: Alkylation; Animals; Arsenites; Ascorbic Acid; Cysteine; Dithiothreitol; Ethylmaleimide; Eukaryotic Cells; Humans; Iodoacetamide; Models, Biological; Oxidation-Reduction; Phosphines; Proteomics; Staining and Labeling; Sulfhydryl Compounds | 2015 |
Effect of 12-week of aerobic exercise on hormones and lipid profile status in adolescent girls with polycystic ovary syndrome: A study during COVID-19.
Topics: Actin Cytoskeleton; Actins; Adaptor Proteins, Signal Transducing; Adenocarcinoma; Adenosine Triphosphate; Adsorption; Adult; Africa, Eastern; Aged; Air Pollutants; Air Pollution; Air Pollution, Indoor; Alcohol Drinking; Allergens; Alzheimer Disease; Amyotrophic Lateral Sclerosis; Animals; Anti-Bacterial Agents; Antibodies; Antibodies, Immobilized; Antigen Presentation; Antigens, CD; Antineoplastic Agents; Antineoplastic Combined Chemotherapy Protocols; Antioxidants; Apoptosis; Aptamers, Nucleotide; Asthma; Asthma, Exercise-Induced; Atrophy; Autophagy; Azoospermia; Bacillus cereus; Bacterial Infections; Beclin-1; Bile Duct Neoplasms; Bile Ducts, Intrahepatic; Biofouling; Biological Monitoring; Biomarkers; Biomarkers, Tumor; Biosensing Techniques; Blastocyst; Bone Neoplasms; Bone Regeneration; Bronchoconstriction; Burkitt Lymphoma; C9orf72 Protein; Campylobacter; Campylobacter Infections; Campylobacter jejuni; Carcinogenesis; Carcinoma, Hepatocellular; Carcinoma, Pancreatic Ductal; Carcinoma, Squamous Cell; Cardiomyopathies; Caregivers; Carmine; Case-Control Studies; Catalysis; Cattle; Cause of Death; CCAAT-Enhancer-Binding Protein-alpha; CD8-Positive T-Lymphocytes; Cefepime; Cell Differentiation; Cell Line, Tumor; Cell Nucleus; Cell Transdifferentiation; Chelating Agents; Chemical and Drug Induced Liver Injury, Chronic; Chemoradiotherapy, Adjuvant; Child; Child, Preschool; China; Chlorquinaldol; Cholangiocarcinoma; Cholera; Chromatin; Clinical Trials as Topic; Cognitive Dysfunction; Cohort Studies; Colonic Neoplasms; Colorectal Neoplasms; Colorimetry; Cooking; Coordination Complexes; COVID-19; Creatinine; CRISPR-Cas Systems; Critical Care; Critical Illness; Cross-Sectional Studies; Cryopreservation; Cryoprotective Agents; Cysteine; Cytokines; Device Removal; Diet; Diet, High-Fat; Diet, Mediterranean; Dietary Supplements; Dimethyl Sulfoxide; Dipeptides; Disease Models, Animal; Dithiothreitol; DNA; DNA Repeat Expansion; DNA, Bacterial; DNA, Complementary; Dopamine; Electrochemical Techniques; Electrodes; Endocannabinoids; Environmental Exposure; Environmental Monitoring; Environmental Pollutants; Enzyme-Linked Immunosorbent Assay; Erlotinib Hydrochloride; Escherichia coli; Escherichia coli O157; Esophageal Neoplasms; Esophagitis, Peptic; Ethylene Glycol; Europium; Exanthema; Fallopian Tubes; Feces; Female; Fertilization in Vitro; Fluoresceins; Fluorescent Dyes; Follicle Stimulating Hormone; Follow-Up Studies; Food Microbiology; Forced Expiratory Volume; Forkhead Transcription Factors; Frontotemporal Dementia; G-Quadruplexes; Galactose; Gastroenteritis; Gastrointestinal Diseases; Gastrointestinal Microbiome; Gastrointestinal Neoplasms; Gastrointestinal Tract; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Genital Neoplasms, Female; Genome-Wide Association Study; Genome, Viral; Genomics; Genotype; Glucose; Glutathione; Glycerol; Gold; Graphite; GTPase-Activating Proteins; Heat-Shock Proteins; Heme Oxygenase-1; Hepacivirus; Hepatitis C; Hepatocytes; Histamine; Histocompatibility Antigens Class II; Hoarseness; Hospice and Palliative Care Nursing; Humans; Hydrogen; Hydrogen Peroxide; Hydrogen Sulfide; Hydroxybenzoates; Hydroxyl Radical; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Hyperthermia, Induced; Hysteroscopy; Immunoassay; Indigo Carmine; Inflammation; Inflammatory Bowel Diseases; Insulin Resistance; Intensive Care Units; Interleukin-11; Interleukin-6; Interleukins; Iodine Radioisotopes; Iran; Iridium; Islets of Langerhans; Kinetics; Lactation; Lactobacillus; Lactobacillus plantarum; Lamins; Latin America; Lead; Lectins; Leukopenia; Ligands; Limit of Detection; Lipopolysaccharides; Lipoprotein Lipase; Liver; Liver Cirrhosis; Liver Neoplasms; Lolium; Luminescent Measurements; Luminol; Lung; Luteinizing Hormone; Macrophages; Magnetic Phenomena; Magnetic Resonance Imaging; Male; Malnutrition; Maltose; Manganese Compounds; Maternal Nutritional Physiological Phenomena; Melatonin; Metabolic Engineering; Metal Nanoparticles; Metallocenes; Metaplasia; Methicillin-Resistant Staphylococcus aureus; Methylation; Mevalonic Acid; Mexico; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microbial Sensitivity Tests; Microbiota; MicroRNAs; Milk; Mitomycin; Molecular Diagnostic Techniques; Molecular Docking Simulation; Monte Carlo Method; Moringa oleifera; Multiple Sclerosis; Muscle Strength; Muscle, Skeletal; Nanocomposites; Nanotubes, Carbon; Neoadjuvant Therapy; Neoplasms; Neurodegenerative Diseases; Neurotransmitter Agents; NF-E2-Related Factor 2; Nickel; Nitrogen Dioxide; Non-alcoholic Fatty Liver Disease; Nucleic Acid Amplification Techniques; Nucleic Acid Hybridization; Nucleocapsid Proteins; Nutritional Status; Obesity; Osteogenesis; Osteosarcoma; Oxidation-Reduction; Oxides; Oxygen; Oxyquinoline; Pain; Palliative Care; Pancreatic Neoplasms; Pandemics; Particulate Matter; Peroxidase; Peroxidases; Phagocytosis; Phaseolus; Photothermal Therapy; Point-of-Care Systems; Polyethyleneimine; Polymers; Polymorphism, Single Nucleotide; Polysomnography; Postoperative Complications; Pregnancy; Pregnant Women; Prenatal Exposure Delayed Effects; Prevalence; Printing, Three-Dimensional; Probability; Probiotics; Prognosis; Prophages; Prospective Studies; Proteomics; Proto-Oncogene Proteins; Pseudomonas aeruginosa; Pseudomonas putida; Pulmonary Disease, Chronic Obstructive; Pulmonary Embolism; Pyridines; Pyrroles; Quality of Life; Quinolones; Rabbits; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Real-Time Polymerase Chain Reaction; Receptors, Histamine; Receptors, Histamine H2; Recombinases; Rectal Neoplasms; Reperfusion Injury; Respiration; Respiratory Function Tests; Respiratory Rate; Respiratory Sounds; Retrospective Studies; rho GTP-Binding Proteins; Risk Assessment; Risk Factors; RNA; RNA, Messenger; RNA, Ribosomal, 16S; Robotic Surgical Procedures; Running; Rural Population; Saccharomyces cerevisiae; Salpingectomy; Sarcopenia; SARS-CoV-2; Seeds; Semen; Sensitivity and Specificity; Sepsis; Shock, Septic; Signal Transduction; Silicon Dioxide; Silver; Sirtuin 1; Skin Neoplasms; Sleep Apnea, Obstructive; Soil; Spain; Spectrum Analysis, Raman; Sperm Retrieval; Spermatozoa; Spirometry; Staphylococcus aureus; STAT3 Transcription Factor; Stereoisomerism; Sterilization, Tubal; Stroke Volume; Sulfadiazine; Sulfites; Superoxide Dismutase; Surface Plasmon Resonance; tau Proteins; Testis; Testosterone; Thioredoxin-Disulfide Reductase; Thyroid Neoplasms; Thyroidectomy; Trans-Activators; Transcription Factor AP-1; Treatment Outcome; Triazoles; Triclosan; Trifluridine; Tumor Microenvironment; Tumor Necrosis Factor-alpha; United States; Uracil; Vagina; Vegetables; Ventricular Function, Left; Ventricular Pressure; Vibrio cholerae; Vietnam; Virulence; Vital Capacity; Vitrification; Walking; Water; Water Pollutants, Radioactive; Whole Genome Sequencing; Wind; YAP-Signaling Proteins; Zeolites; Zinc Oxide | 2023 |
1 trial(s) available for cysteine and dithiothreitol
| Article | Year |
|---|---|
Effect of 12-week of aerobic exercise on hormones and lipid profile status in adolescent girls with polycystic ovary syndrome: A study during COVID-19.
Topics: Actin Cytoskeleton; Actins; Adaptor Proteins, Signal Transducing; Adenocarcinoma; Adenosine Triphosphate; Adsorption; Adult; Africa, Eastern; Aged; Air Pollutants; Air Pollution; Air Pollution, Indoor; Alcohol Drinking; Allergens; Alzheimer Disease; Amyotrophic Lateral Sclerosis; Animals; Anti-Bacterial Agents; Antibodies; Antibodies, Immobilized; Antigen Presentation; Antigens, CD; Antineoplastic Agents; Antineoplastic Combined Chemotherapy Protocols; Antioxidants; Apoptosis; Aptamers, Nucleotide; Asthma; Asthma, Exercise-Induced; Atrophy; Autophagy; Azoospermia; Bacillus cereus; Bacterial Infections; Beclin-1; Bile Duct Neoplasms; Bile Ducts, Intrahepatic; Biofouling; Biological Monitoring; Biomarkers; Biomarkers, Tumor; Biosensing Techniques; Blastocyst; Bone Neoplasms; Bone Regeneration; Bronchoconstriction; Burkitt Lymphoma; C9orf72 Protein; Campylobacter; Campylobacter Infections; Campylobacter jejuni; Carcinogenesis; Carcinoma, Hepatocellular; Carcinoma, Pancreatic Ductal; Carcinoma, Squamous Cell; Cardiomyopathies; Caregivers; Carmine; Case-Control Studies; Catalysis; Cattle; Cause of Death; CCAAT-Enhancer-Binding Protein-alpha; CD8-Positive T-Lymphocytes; Cefepime; Cell Differentiation; Cell Line, Tumor; Cell Nucleus; Cell Transdifferentiation; Chelating Agents; Chemical and Drug Induced Liver Injury, Chronic; Chemoradiotherapy, Adjuvant; Child; Child, Preschool; China; Chlorquinaldol; Cholangiocarcinoma; Cholera; Chromatin; Clinical Trials as Topic; Cognitive Dysfunction; Cohort Studies; Colonic Neoplasms; Colorectal Neoplasms; Colorimetry; Cooking; Coordination Complexes; COVID-19; Creatinine; CRISPR-Cas Systems; Critical Care; Critical Illness; Cross-Sectional Studies; Cryopreservation; Cryoprotective Agents; Cysteine; Cytokines; Device Removal; Diet; Diet, High-Fat; Diet, Mediterranean; Dietary Supplements; Dimethyl Sulfoxide; Dipeptides; Disease Models, Animal; Dithiothreitol; DNA; DNA Repeat Expansion; DNA, Bacterial; DNA, Complementary; Dopamine; Electrochemical Techniques; Electrodes; Endocannabinoids; Environmental Exposure; Environmental Monitoring; Environmental Pollutants; Enzyme-Linked Immunosorbent Assay; Erlotinib Hydrochloride; Escherichia coli; Escherichia coli O157; Esophageal Neoplasms; Esophagitis, Peptic; Ethylene Glycol; Europium; Exanthema; Fallopian Tubes; Feces; Female; Fertilization in Vitro; Fluoresceins; Fluorescent Dyes; Follicle Stimulating Hormone; Follow-Up Studies; Food Microbiology; Forced Expiratory Volume; Forkhead Transcription Factors; Frontotemporal Dementia; G-Quadruplexes; Galactose; Gastroenteritis; Gastrointestinal Diseases; Gastrointestinal Microbiome; Gastrointestinal Neoplasms; Gastrointestinal Tract; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Genital Neoplasms, Female; Genome-Wide Association Study; Genome, Viral; Genomics; Genotype; Glucose; Glutathione; Glycerol; Gold; Graphite; GTPase-Activating Proteins; Heat-Shock Proteins; Heme Oxygenase-1; Hepacivirus; Hepatitis C; Hepatocytes; Histamine; Histocompatibility Antigens Class II; Hoarseness; Hospice and Palliative Care Nursing; Humans; Hydrogen; Hydrogen Peroxide; Hydrogen Sulfide; Hydroxybenzoates; Hydroxyl Radical; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Hyperthermia, Induced; Hysteroscopy; Immunoassay; Indigo Carmine; Inflammation; Inflammatory Bowel Diseases; Insulin Resistance; Intensive Care Units; Interleukin-11; Interleukin-6; Interleukins; Iodine Radioisotopes; Iran; Iridium; Islets of Langerhans; Kinetics; Lactation; Lactobacillus; Lactobacillus plantarum; Lamins; Latin America; Lead; Lectins; Leukopenia; Ligands; Limit of Detection; Lipopolysaccharides; Lipoprotein Lipase; Liver; Liver Cirrhosis; Liver Neoplasms; Lolium; Luminescent Measurements; Luminol; Lung; Luteinizing Hormone; Macrophages; Magnetic Phenomena; Magnetic Resonance Imaging; Male; Malnutrition; Maltose; Manganese Compounds; Maternal Nutritional Physiological Phenomena; Melatonin; Metabolic Engineering; Metal Nanoparticles; Metallocenes; Metaplasia; Methicillin-Resistant Staphylococcus aureus; Methylation; Mevalonic Acid; Mexico; Mice; Mice, Inbred C57BL; Mice, Transgenic; Microbial Sensitivity Tests; Microbiota; MicroRNAs; Milk; Mitomycin; Molecular Diagnostic Techniques; Molecular Docking Simulation; Monte Carlo Method; Moringa oleifera; Multiple Sclerosis; Muscle Strength; Muscle, Skeletal; Nanocomposites; Nanotubes, Carbon; Neoadjuvant Therapy; Neoplasms; Neurodegenerative Diseases; Neurotransmitter Agents; NF-E2-Related Factor 2; Nickel; Nitrogen Dioxide; Non-alcoholic Fatty Liver Disease; Nucleic Acid Amplification Techniques; Nucleic Acid Hybridization; Nucleocapsid Proteins; Nutritional Status; Obesity; Osteogenesis; Osteosarcoma; Oxidation-Reduction; Oxides; Oxygen; Oxyquinoline; Pain; Palliative Care; Pancreatic Neoplasms; Pandemics; Particulate Matter; Peroxidase; Peroxidases; Phagocytosis; Phaseolus; Photothermal Therapy; Point-of-Care Systems; Polyethyleneimine; Polymers; Polymorphism, Single Nucleotide; Polysomnography; Postoperative Complications; Pregnancy; Pregnant Women; Prenatal Exposure Delayed Effects; Prevalence; Printing, Three-Dimensional; Probability; Probiotics; Prognosis; Prophages; Prospective Studies; Proteomics; Proto-Oncogene Proteins; Pseudomonas aeruginosa; Pseudomonas putida; Pulmonary Disease, Chronic Obstructive; Pulmonary Embolism; Pyridines; Pyrroles; Quality of Life; Quinolones; Rabbits; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Real-Time Polymerase Chain Reaction; Receptors, Histamine; Receptors, Histamine H2; Recombinases; Rectal Neoplasms; Reperfusion Injury; Respiration; Respiratory Function Tests; Respiratory Rate; Respiratory Sounds; Retrospective Studies; rho GTP-Binding Proteins; Risk Assessment; Risk Factors; RNA; RNA, Messenger; RNA, Ribosomal, 16S; Robotic Surgical Procedures; Running; Rural Population; Saccharomyces cerevisiae; Salpingectomy; Sarcopenia; SARS-CoV-2; Seeds; Semen; Sensitivity and Specificity; Sepsis; Shock, Septic; Signal Transduction; Silicon Dioxide; Silver; Sirtuin 1; Skin Neoplasms; Sleep Apnea, Obstructive; Soil; Spain; Spectrum Analysis, Raman; Sperm Retrieval; Spermatozoa; Spirometry; Staphylococcus aureus; STAT3 Transcription Factor; Stereoisomerism; Sterilization, Tubal; Stroke Volume; Sulfadiazine; Sulfites; Superoxide Dismutase; Surface Plasmon Resonance; tau Proteins; Testis; Testosterone; Thioredoxin-Disulfide Reductase; Thyroid Neoplasms; Thyroidectomy; Trans-Activators; Transcription Factor AP-1; Treatment Outcome; Triazoles; Triclosan; Trifluridine; Tumor Microenvironment; Tumor Necrosis Factor-alpha; United States; Uracil; Vagina; Vegetables; Ventricular Function, Left; Ventricular Pressure; Vibrio cholerae; Vietnam; Virulence; Vital Capacity; Vitrification; Walking; Water; Water Pollutants, Radioactive; Whole Genome Sequencing; Wind; YAP-Signaling Proteins; Zeolites; Zinc Oxide | 2023 |
777 other study(ies) available for cysteine and dithiothreitol
| Article | Year |
|---|---|
Interactions between cysteine residues as probes of protein conformation: the disulphide bond between Cys-14 and Cys-38 of the pancreatic trypsin inhibitor.
Topics: Animals; Binding Sites; Cysteine; Disulfides; Dithiothreitol; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Pancreas; Protein Binding; Thermodynamics; Trypsin Inhibitors | 1975 |
Requirement of an essential thiol group and ferric iron for the activity of the progesterone-induced porcine uterine purple phosphatase.
Topics: Acid Phosphatase; Animals; Color; Cyanides; Cysteine; Dithionite; Dithiothreitol; Electron Spin Resonance Spectroscopy; Enzyme Activation; Enzyme Induction; Female; Ferric Compounds; Glutathione; Hydrogen-Ion Concentration; Iron; Kinetics; Mercaptoethanol; Progesterone; Sulfhydryl Compounds; Sulfites; Uterus | 1976 |
Activation of hepatic guanylate cyclase by N-methyl-N'-nitro-N-nitrosoguanidine. Effects of thiols, N-ethylmaleimide, and divalent cations.
Topics: Anaerobiosis; Animals; Ascorbic Acid; Cysteine; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Glutathione; Guanylate Cyclase; In Vitro Techniques; Kinetics; Liver; Magnesium; Male; Manganese; Methylnitronitrosoguanidine; Rats; Sulfhydryl Compounds | 1977 |
Ascorbic acid modulation of splenic cell cyclic GMP metabolism.
Topics: Animals; Ascorbic Acid; Ascorbic Acid Deficiency; Cyclic GMP; Cysteine; Dehydroascorbic Acid; Dithiothreitol; Guanylate Cyclase; Guinea Pigs; Spleen; Time Factors | 1979 |
Activation of fungal alpha-amylase by dithioerythritol.
Topics: alpha-Amylases; Amylases; Aspergillus oryzae; Cell-Free System; Cysteine; Dithioerythritol; Dithiothreitol; Enzyme Activation; Iodoacetamide; Mercaptoethanol | 1979 |
Effect of certain sulphydryl compounds and dimethyl sulphoxide on potassium release by certain amines and proteins from Pseudomonas aeruginosa.
Topics: Amines; Ammonium Chloride; Cysteine; Dimethyl Sulfoxide; Dithiothreitol; Mercaptoethanol; Potassium; Proteins; Pseudomonas aeruginosa; Sulfhydryl Compounds | 1977 |
Action of cytochalasin A, a sulfhydryl-reactive agent, on sugar metabolism and membrane-bound adenosine triphosphatase of yeast.
Topics: Adenosine Triphosphatases; Cysteine; Cytochalasins; Dicyclohexylcarbodiimide; Dithiothreitol; Glucose; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycolysis; Iodoacetates; Oligomycins; Saccharomyces; Sugar Phosphates | 1975 |
Effect of ozone on erythrocyte membrane adenosine triphosphatase.
Topics: Adenosine Triphosphatases; Ascorbic Acid; Cell Membrane; Cysteine; Dithiothreitol; Erythrocytes; Humans; In Vitro Techniques; Kinetics; Ouabain; Ozone | 1976 |
Non-cooperative response of actin-cystein 373 in cooperatively behaving regulated actin filaments.
Topics: Actins; Adenosine Triphosphatases; Animals; Cysteine; Dithiothreitol; Ethylmaleimide; Fluorescent Dyes; Iodoacetamide; Kinetics; Magnesium; Naphthalenesulfonates; Protein Binding; Spectrometry, Fluorescence | 1979 |
Specific inhibition of protein kinase by diazenedicarboxylic acid bis-(N,N-dimethylamide).
Topics: Animals; Azo Compounds; Cattle; Cysteine; Dialysis; Dithiothreitol; Formamides; Glutathione; Histones; Hydrazines; Kidney; Phosphorus Radioisotopes; Phosphotransferases; Protein Kinase Inhibitors; Swine | 1975 |
Hydrolysis of triphosphoinositides by a soluble fraction of Crithidia fasciculata.
Topics: Animals; Calcium; Cetrimonium Compounds; Cysteine; Dithiothreitol; Enzyme Activation; Fluorides; Glutathione; Kinetics; Magnesium; Phosphatidylinositols; Phosphoric Diester Hydrolases; Phosphoric Monoester Hydrolases; Trypanosoma | 1976 |
Effects of disulfide bond reduction on the excitatory and inhibitory postsynaptic responses of Aplysia ganglion cells.
Topics: Acetylcholine; Acetylcholinesterase; Affinity Labels; Animals; Cholinergic Fibers; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Dose-Response Relationship, Drug; gamma-Aminobutyric Acid; Ganglia; Mollusca; Neural Conduction; Neural Inhibition; Nitrobenzoates; Oxidation-Reduction; Receptors, Dopamine; Receptors, Muscarinic; Receptors, Neurotransmitter; Receptors, Nicotinic; Structure-Activity Relationship | 1976 |
Artificial hybrid protein containing a toxic protein fragment and a cell membrane receptor-binding moiety in a disulfide conjugate. II. Biochemical and biologic properties of diphtheria toxin fragment A-S-S-human placental lactogen.
Topics: Adenosine Diphosphate Sugars; Animals; Binding Sites; Cell Membrane; Cycloheximide; Cysteine; Diphtheria Toxin; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Kinetics; Macromolecular Substances; Mathematics; Molecular Weight; Pentosyltransferases; Placental Lactogen; Protein Binding; Receptors, Cell Surface | 1977 |
Regulation of dimorphism in the pathogenic fungus Histoplasma capsulatum.
Topics: 4-Chloromercuribenzenesulfonate; Biological Transport; Cell Differentiation; Cyclic AMP; Cysteine; Cystine; Dithiothreitol; Histoplasma; Oxidation-Reduction; Oxygen Consumption; Temperature | 1977 |
The action of thiols on lymphocyte membranes.
Topics: Animals; Cell Membrane; Cyclic AMP; Cysteine; Dithiothreitol; Epinephrine; Female; Glutathione; Lymphocytes; Rats; Rats, Inbred Strains; Time Factors | 1976 |
Inhibition by aldehydes as a possible further mechanism for glucose-6-phosphatase inactivation during CCl4-poisoning.
Topics: Aldehydes; Animals; Carbon Tetrachloride Poisoning; Cysteine; Dithiothreitol; Glucose-6-Phosphatase; Glutathione; Glycine; In Vitro Techniques; Male; Maleates; Microsomes, Liver; Rats; Time Factors | 1977 |
Chemical modification of the beta adrenergic receptors coupled with adenylate cyclase by disulfide bridge-reducing agents.
Topics: Adenylyl Cyclases; Alprenolol; Animals; Cells, Cultured; Cysteine; Dithiothreitol; Female; In Vitro Techniques; Isoproterenol; Kinetics; Mercaptoethanol; Oxidation-Reduction; Rats; Receptors, Adrenergic; Receptors, Adrenergic, beta; Stereoisomerism; Time Factors | 1978 |
On the modification of adenosine kinase by thiols.
Topics: Adenosine Kinase; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Kinetics; Mercaptoethanol; Phosphotransferases; Saccharomyces cerevisiae; Sulfhydryl Compounds | 1978 |
The role of reduced glutathione in thyroglobulin proteolysis iv vitro.
Topics: Animals; Ascorbic Acid; Caseins; Cysteine; Dithiothreitol; Dogs; Glutathione; Hemoglobins; Hydrogen-Ion Concentration; Hydrolysis; Insulin; Mercaptoethanol; Peptide Hydrolases; Stimulation, Chemical; Temperature; Thyroglobulin; Thyroid Gland; Time Factors | 1975 |
Rat liver cysteine sulfinate decarboxylase: purification, new appraisal of the molecular weight and determination of catalytic properties.
Topics: Animals; Borohydrides; Carboxy-Lyases; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Kinetics; Liver; Male; Molecular Weight; Norepinephrine; Penicillamine; Protein Conformation; Pyridoxal Phosphate; Rats; Sulfhydryl Compounds; Sulfinic Acids; Temperature | 1975 |
Effects of sulfhydryl agents on the activation of tryptophan-5-monooxygenase from bovine pineal glands.
Topics: 2,2'-Dipyridyl; Animals; Ascorbic Acid; Borohydrides; Cattle; Cysteine; Dithionite; Dithiothreitol; Enzyme Activation; Glutathione; Hydrogen-Ion Concentration; Iron; Kinetics; Mercaptoethanol; Mixed Function Oxygenases; Phenanthrolines; Pineal Gland; Sulfhydryl Reagents; Tryptophan Hydroxylase | 1975 |
Some properties and subcellular distribution of palmitoyl-CoA hydrolase and phosphatidate phosphohydrolase in rabbit hearts.
Topics: Animals; Carnitine; Cattle; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Hydrolysis; Male; Microsomes; Muscle Proteins; Myocardium; Palmitic Acids; Phospholipids; Phosphoric Monoester Hydrolases; Rabbits; Subcellular Fractions; Thiolester Hydrolases; Time Factors | 1975 |
Studies on the mechanism of the NADPH-catalyzed peroxidation of endogenous microsomal lipid.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Chelating Agents; Cysteine; Diphosphates; Dithiothreitol; Enzyme Activation; Glutathione; Kinetics; Lipids; Male; Microsomes, Liver; NADP; Oxidation-Reduction; Peroxidases; Phosphates; Protein Binding; Rats; Time Factors | 1975 |
3-Hydroxy-3-methylglutaryl coenzyme A synthase. Evidence for an acetyl-S-enzyme intermediate and identification of a cysteinyl sulfhydryl as the site of acetylation.
Topics: Acetylation; Animals; Binding Sites; Birds; Chromatography, Ion Exchange; Chromatography, Thin Layer; Cysteamine; Cysteine; Dithiothreitol; Electrophoresis, Paper; Electrophoresis, Polyacrylamide Gel; Glutathione; Hydroxymethylglutaryl-CoA Synthase; Kinetics; Liver; Oxo-Acid-Lyases; Pronase; Protein Binding; Time Factors | 1975 |
Interaction of Treponema pallidum (Nichols strain) with cultured mammalian cells: effects of oxygen, reducing agents, serum supplements, and different cell types.
Topics: Blood; Cell Line; Culture Media; Cysteine; Dithiothreitol; Glutathione; Movement; Oxygen; Serum Albumin, Bovine; Treponema pallidum | 1977 |
The threonine-sensitive homoserine dehydrogenase and aspartokinase activities of Escherichia coli K12. Carboxymethylation of a unique cysteine induces a conformational change of the enzyme.
Topics: Alkylation; Aspartokinase Homoserine Dehydrogenase; Cysteine; Dithiothreitol; Escherichia coli; Homoserine; Iodoacetates; Kinetics; Multienzyme Complexes; Protein Conformation | 1978 |
Influence of oxygen tension, sulfhydryl compounds, and serum on the motility and virulence of Treponema pallidum (Nichols strain) in a cell-free system.
Topics: Blood; Cell-Free System; Cells, Cultured; Cysteine; Dithiothreitol; Glutathione; Movement; Oxygen; Sulfhydryl Compounds; Treponema pallidum | 1978 |
The effect of reducing and other agents on the motility of Treponema pallidum in an acellular culture medium.
Topics: Culture Media; Cysteine; Dithiothreitol; Glutathione; Movement; Oxidation-Reduction; Serum Albumin, Bovine; Thioglycolates; Treponema pallidum | 1979 |
Effect of thiols on macroconidia of Fusarium sulphureum.
Topics: Amino Acids; Cysteine; Dithioerythritol; Dithiothreitol; Fusarium; Lipids; Mercaptoethanol; Spores, Fungal; Sulfhydryl Compounds; Thiourea | 1979 |
Dose-related enhancement of erythropoiesis by sulfhydryl compounds and its reversal with a thiol inhibitor.
Topics: Animals; Bone Marrow; Cysteine; Dithiothreitol; Erythropoiesis; Mercaptoethanol; Rats; Sulfhydryl Compounds | 1979 |
Glutathione and gamma-glutamylcysteine in whole blood, plasma and erythrocytes.
Topics: Adult; Cysteine; Dithiothreitol; Erythrocytes; Glutathione; Humans; Oxidation-Reduction; Plasma | 1978 |
Evidence for two sites of inhibition of photosynthetic electron transport by dibromothymoquinone.
Topics: Binding Sites; Chloroplasts; Cyanides; Cysteine; Depression, Chemical; Dibromothymoquinone; Dithiothreitol; Electron Transport; Ferricyanides; Mercury; Phenylenediamines; Photosynthesis; Plants; Quinones; Serum Albumin, Bovine | 1978 |
Purification and properties of porcine platelet aggregating factor.
Topics: Amino Acids; Animals; Blood Coagulation Factors; Carbohydrates; Cysteine; Dithiothreitol; Factor VIII; Guanidines; Lysine; Platelet Aggregation; Swine; Urea | 1978 |
Growth of clinical isolates of anaerobic bacteria on agar media: effects of media composition, storage conditions, and reduction under anaerobic conditions.
Topics: Agar; Anaerobiosis; Bacteria; Bacterial Infections; Cysteine; Dithiothreitol; Humans; Palladium | 1978 |
Molecular organization in bacterial cell membranes. Sulfhydryl groups and disulfide bridges in Streptomyces albus and Escherichia coli K 12 cytoplasmic membranes.
Topics: Bacterial Proteins; Binding Sites; Cell Membrane; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Macromolecular Substances; Protein Binding; Protein Conformation; Streptomyces; Sulfhydryl Compounds; Sulfhydryl Reagents | 1976 |
[Prevention of 3,4-benzpyrene carcinogenesis by naturally occurring and synthetic compounds].
Topics: Aconitic Acid; Animals; Antineoplastic Agents; Benzopyrenes; Cadaverine; Carcinogens; Cysteine; Dicarboxylic Acids; Dithiothreitol; Ethanolamines; Female; Fumarates; Maleates; Mice; Neoplasms, Experimental; Putrescine; Spermidine; Spermine; Sulfhydryl Compounds; Tricarboxylic Acids | 1975 |
Prostaglandin endoperoxide E isomerase from bovine vesicular gland microsomes, a glutathione-requiring enzyme.
Topics: Animals; Cattle; Cysteine; Dithiothreitol; Drug Stability; Glutathione; Isomerases; Kinetics; Male; Mercaptoethanol; Microsomes; Peroxides; Prostaglandins E; Seminal Vesicles | 1977 |
Stabilization and induction of a lipid peroxidation inhibitor present in the soluble fraction of rat liver homogenates.
Topics: Animals; Cysteine; Dithiothreitol; Glutathione; In Vitro Techniques; Lipid Metabolism; Liver; Male; Mercaptoethanol; Methylcholanthrene; Peroxides; Rats; Subcellular Fractions | 1977 |
The action of cysteine on PHA-stimulation of lymphocytes. I. Influence of DNA synthesis.
Topics: Animals; Cell Survival; Cysteine; Dithiothreitol; DNA; Glutathione; Lymphocyte Activation; Rats; Thymidine | 1977 |
Alkylthiolation. Evidence for involvement in cell division.
Topics: Animals; Cell Division; Cell Line; Cysteine; Disulfides; Dithiothreitol; Glutathione; Kinetics; Leukemia L1210; Mice; Structure-Activity Relationship; Sulfhydryl Compounds | 1976 |
Studies on the cholesterol ester hydrolase of Trogoderma (Coleoptera).
Topics: Animals; Carboxylic Ester Hydrolases; Cholesterol; Cholesterol Esters; Coleoptera; Cysteine; Dithiothreitol; Glutathione; Hydroxymercuribenzoates; Iodoacetates; Sterol Esterase | 1976 |
Effects of nutritional characteristics of Streptococcus agalactiae on inhibition of growth by lactoperoxidase-thiocyanate-hydrogen peroxide in chemically defined culture medium.
Topics: Culture Media; Cysteine; Cystine; Dithiothreitol; Glutathione; Hydrogen Peroxide; Lactoperoxidase; Oxygen Consumption; Peroxidases; Peroxides; Streptococcus; Thiocyanates | 1976 |
Cytotoxicity of cysteine in culture media.
Topics: Animals; Blood; Cattle; Cell Division; Cell Line; Cells, Cultured; Chloromercuribenzoates; Culture Media; Cysteine; Cytotoxicity Tests, Immunologic; Dithiothreitol; In Vitro Techniques; Mercaptoethanol; Pyruvates; Temperature | 1976 |
N-ethylmaleimide-induced conductance changes in the giant neuron of Aplysia.
Topics: Action Potentials; Animals; Cell Membrane; Cell Membrane Permeability; Chlorides; Cysteine; Dithiothreitol; Ethylmaleimide; Ganglia; In Vitro Techniques; Membrane Potentials; Mollusca; Neurons; Potassium; Sodium | 1975 |
Further studies on acetamidination as a technique for preparation of a biologically valid 3-H-labeled tracer for parathyroid hormone.
Topics: Adenylyl Cyclases; Amidines; Animals; Biological Assay; Calcium; Chromatography, Ion Exchange; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Isotope Labeling; Parathyroid Glands; Parathyroid Hormone; Phosphates; Rats; Tritium | 1975 |
DNA biosynthesis in rat liver mitochondria. Inhibition by sulfhydryl compounds and stimulation by cytoplasmic proteins.
Topics: Animals; Cysteine; Cytoplasm; Dithiothreitol; DNA, Mitochondrial; Ethidium; Glutathione; Liver Regeneration; Mercaptoethanol; Mice; Mitochondria, Liver; Proteins; Rats; Sulfhydryl Compounds | 1975 |
Creatine kinase reactivation by thiol compounds.
Topics: Creatine Kinase; Cysteine; Dithioerythritol; Dithiothreitol; Enzyme Activation; Glutathione; Humans; Kinetics; Mercaptoethanol; Sulfhydryl Compounds | 1975 |
Determination of 3-mercaptopyruvate-cysteine disulfide, a product of oxidative deamination of L-cystine by L-amino acid oxidase.
Topics: Amino Acid Oxidoreductases; Chromatography, Ion Exchange; Cysteine; Cystine; Disulfides; Dithiothreitol; Kinetics; L-Lactate Dehydrogenase; Pyruvates; Snake Venoms; Structure-Activity Relationship; Sulfhydryl Compounds; Time Factors | 1975 |
A type kappa Bence Jones protein containing a cysteinyl residue in the variable region.
Topics: Alkylation; Amino Acids; Ammonium Sulfate; Bence Jones Protein; Chromatography, Ion Exchange; Circular Dichroism; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin Fragments; Iodoacetamide; Iodoacetates; Molecular Weight; Multiple Myeloma; Nitrobenzoates; Sulfhydryl Compounds | 1975 |
Glutamate decarboxylase from barley embryos and roots. General properties and the occurrence of three enzymic forms.
Topics: Alcohol Oxidoreductases; Carboxy-Lyases; Catalase; Cysteine; Dithiothreitol; Edible Grain; Glutamate Decarboxylase; Hordeum; Isoenzymes; Kinetics; Mercaptoethanol; Molecular Weight; Oxygen | 1975 |
Induction of nuclear decondensation of mammalian spermatozoa in vitro.
Topics: Animals; Cell Nucleus; Cricetinae; Cysteine; Dithiothreitol; Dogs; Guinea Pigs; Hydroxides; In Vitro Techniques; Male; Mercaptoethanol; Rabbits; Rats; Sodium Dodecyl Sulfate; Spermatozoa; Sulfides; Thioglycolates | 1975 |
Significant enhancement of T-strain mycoplasmal growth on supplemented Shepard's A6 differential agar.
Topics: Agar; Cysteine; Dithiothreitol; Humans; Mercaptoethanol; Mycoplasma; Mycoplasma Infections; Thioglycolates | 1975 |
In vitro inhibition of polyphenylalanine synthesis by bromotrichloromethane. Evidence for an oxidative mechanism.
Topics: Animals; Bromotrichloromethane; Chloroform; Cysteine; Depression, Chemical; Dithiothreitol; In Vitro Techniques; Magnesium; Male; Malondialdehyde; Mitochondria, Liver; Oxidation-Reduction; Peptide Biosynthesis; Peptide Chain Initiation, Translational; Phenylalanine; Rats; Sulfhydryl Compounds | 1975 |
The two-disulphide intermediates and the folding pathway of reduced pancreatic trypsin inhibitor.
Topics: Chromatography, Gel; Chromatography, Ion Exchange; Cysteine; Disulfides; Dithiothreitol; Electrophoresis; Glutathione; Iodoacetates; Kinetics; Models, Chemical; Models, Molecular; Oxidation-Reduction; Protein Conformation; Thermolysin; Trypsin Inhibitors | 1975 |
A phospholipid-deacylating system of bacteria active in a frozen medium.
Topics: Calcium; Calorimetry; Cysteine; Dithiothreitol; Eubacterium; Freezing; Glutathione; Kinetics; Magnesium; Manganese; Mercaptoethanol; Oleic Acids; Phosphatidylcholines; Phospholipases; Sodium Dodecyl Sulfate; Temperature | 1976 |
Decrease in heart mitochondrial creatine kinase activity due to oxygen free radicals.
Topics: Animals; Creatine Kinase; Cysteine; Dithiothreitol; Free Radicals; Hydrogen Peroxide; Male; Mitochondria, Heart; Rats; Rats, Sprague-Dawley; Sulfhydryl Compounds; Superoxides; Xanthine; Xanthine Oxidase; Xanthines | 1992 |
Correlation of haemagglutination activity with trypsin-like protease activity of Porphyromonas gingivalis.
Topics: Animals; Cysteine; Dithiothreitol; Ethylmaleimide; Fusobacterium nucleatum; Gingiva; Hemagglutination; Humans; Macaca fascicularis; Mutagens; Nitrosoguanidines; Phenotype; Phenylmethylsulfonyl Fluoride; Porphyromonas gingivalis; Tosyllysine Chloromethyl Ketone; Trypsin | 1992 |
Involvement of thiol groups in the reaction mechanism of Mn(2+)-activated alkaline p-nitrophenylphosphate phosphatase of the extreme halophilic archaebacterium Halobacterium halobium.
Topics: 4-Nitrophenylphosphatase; Catalysis; Cations, Divalent; Cysteine; Dithiothreitol; Enzyme Activation; Halobacterium salinarum; Hydrogen-Ion Concentration; Hydroxymercuribenzoates; Kinetics; Manganese; Mercaptoethanol; Potassium Cyanide; Sulfhydryl Compounds | 1992 |
Disulfide bond-coupled folding of bovine pancreatic trypsin inhibitor derivatives missing one or two disulfide bonds.
Topics: Alanine; Alkylation; Aprotinin; Cysteine; Disulfides; Dithiothreitol; Glutathione; Kinetics; Mass Spectrometry; Oxidation-Reduction; Protein Conformation | 1992 |
Different roles of the two disulfide bonds of the cysteine proteinase inhibitor, chicken cystatin, for the conformation of the active protein.
Topics: Animals; Chickens; Circular Dichroism; Cystatins; Cysteine; Cystine; Dithiothreitol; Isoelectric Point; Protein Conformation; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Thioredoxins | 1992 |
A novel mechanism for regulation of vacuolar acidification.
Topics: Animals; Brain; Cattle; Clathrin; Coated Pits, Cell-Membrane; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Hydrogen-Ion Concentration; Oxidation-Reduction; Proton-Translocating ATPases; Vacuoles | 1992 |
Oxalate production from glyoxylate by lactate dehydrogenase in vitro: inhibition by reduced glutathione, cysteine, cysteamine.
Topics: Animals; Cysteamine; Cysteine; Dithiothreitol; Glutathione; Glyoxylates; L-Lactate Dehydrogenase; Liver; Muscle Proteins; Oxalates; Rabbits; Rats; Sulfhydryl Reagents | 1992 |
Intestinal absorption and lymphatic transport of peroxidized lipids in rats: effect of exogenous GSH.
Topics: Animals; Biological Transport; Cysteine; Dithiothreitol; Glutathione; Intestinal Absorption; Intestinal Mucosa; Lipid Peroxides; Lymphatic System; Osmolar Concentration; Rats | 1992 |
Regulation of the cellular stress response by reactive electrophiles. The role of covalent binding and cellular thiols in transcriptional activation of the 70-kilodalton heat shock protein gene by nephrotoxic cysteine conjugates.
Topics: Aminooxyacetic Acid; Animals; Calcium; Cell Line; Cycloheximide; Cysteine; Dactinomycin; Dithiothreitol; Gene Expression Regulation; Heat-Shock Proteins; Hydrocarbons, Fluorinated; Kidney; Kinetics; Lipid Peroxidation; Molecular Weight; Protein Conformation; RNA, Messenger; Signal Transduction; Transcription, Genetic | 1992 |
Cysteine 254 of the 73-kDa A subunit is responsible for inhibition of the coated vesicle (H+)-ATPase upon modification by sulfhydryl reagents.
Topics: Amino Acid Sequence; Animals; Binding Sites; Brain; Cattle; Clathrin; Coated Pits, Cell-Membrane; Cysteine; Dithiothreitol; Ethylmaleimide; Kinetics; Macromolecular Substances; Molecular Sequence Data; Molecular Weight; Proton-Translocating ATPases; Sulfhydryl Reagents | 1992 |
Attenuation of immunologic reactivity of lipoprotein(a) by thiols and cysteine-containing compounds. Structural implications.
Topics: Acetylcysteine; Apolipoproteins A; Apolipoproteins B; Blotting, Western; Cysteine; Dithiothreitol; Enzyme-Linked Immunosorbent Assay; Homocysteine; Humans; Lipoprotein(a); Lipoproteins; Male; Mercaptoethanol; Molecular Structure; Sulfhydryl Compounds | 1992 |
Cysteine 647 in the insulin receptor is required for normal covalent interaction between alpha- and beta-subunits and signal transduction.
Topics: Affinity Labels; Animals; Base Sequence; CHO Cells; Cricetinae; Cross-Linking Reagents; Cysteine; Dithiothreitol; DNA; Electrophoresis, Polyacrylamide Gel; Insulin; Iodine Radioisotopes; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Phosphorylation; Precipitin Tests; Receptor, Insulin; Serine; Signal Transduction; Transfection | 1992 |
Activation of the growth arrest and DNA damage-inducible gene gadd 153 by nephrotoxic cysteine conjugates and dithiothreitol.
Topics: Aminooxyacetic Acid; Calcium; Cations, Divalent; Cell Line; Cycloheximide; Cysteine; Dactinomycin; Dithiothreitol; DNA Damage; Drug Interactions; Kidney; Protein Biosynthesis; RNA, Messenger; Transcription, Genetic | 1992 |
In vitro processing of insulin for recognition by murine T cells results in the generation of A chains with free CysSH.
Topics: Animals; Antigen-Presenting Cells; Chromatography, Gel; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Glutathione; In Vitro Techniques; Insulin; Interleukin-3; Mercaptoethanol; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; Oxidation-Reduction; T-Lymphocytes | 1992 |
Vanadyl causes hydroxyl radical mediated degradation of deoxyribose.
Topics: Catalase; Cysteine; Deoxyribose; Dithiothreitol; Ethanol; HEPES; Hydrogen Peroxide; Hydroxides; Hydroxyl Radical; Iron; NAD; Oxidation-Reduction; Oxygen; Superoxide Dismutase; Vanadates | 1991 |
Calcium release from pituitary secretory granules: modulation by thiols, disulfides, and dihydropyridine calcium channel blockers.
Topics: 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; Animals; Calcium; Calcium Channel Blockers; Cattle; Cysteine; Cystine; Cytoplasmic Granules; Dihydropyridines; Disulfides; Dithiothreitol; Glutathione; Hydrogen-Ion Concentration; Mercaptoethanol; Nifedipine; Nimodipine; Pituitary Gland; Potassium Chloride; Sulfhydryl Compounds | 1990 |
Inactivation of purified phenylalanine hydroxylase by dithiothreitol.
Topics: Anaerobiosis; Animals; Ascorbic Acid; Catalase; Cysteine; Dithiothreitol; Glutathione; Kinetics; Liver; Mercaptoethanol; Norepinephrine; Phenylalanine Hydroxylase; Rats | 1992 |
On kynureninase activity.
Topics: Animals; Apoenzymes; Benzothiazoles; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Hydrolases; Kynurenine; Liver; Magnesium; Male; Phenylpyruvic Acids; Pyridoxal Phosphate; Rats; Rats, Inbred Strains; Tryptophan; Tyrosine; Xanthurenates | 1991 |
Detection of carboxymethylmetallothionein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Topics: Animals; Autoradiography; Cells, Cultured; Cysteine; Cytosol; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Indicators and Reagents; Iodoacetates; Iodoacetic Acid; Liver; Metallothionein; Sodium Dodecyl Sulfate; Sulfur Radioisotopes | 1991 |
Reductive cleavage and regeneration of the disulfide bonds in Streptomyces subtilisin inhibitor (SSI) as studied by the carbonyl 13C NMR resonances of cysteinyl residues.
Topics: Bacterial Proteins; Carbon Isotopes; Cysteine; Cystine; Dithiothreitol; Magnetic Resonance Spectroscopy; Models, Molecular; Mutagenesis, Site-Directed; Oxidation-Reduction; Proline; Protein Conformation; Serine Proteinase Inhibitors; Streptomyces; Subtilisins | 1991 |
Activation of chromium(VI) by thiols results in chromium(V) formation, chromium binding to DNA and altered DNA conformation.
Topics: Chromium; Cysteine; Dithiothreitol; DNA; DNA Damage; Electron Spin Resonance Spectroscopy; Electrophoresis, Agar Gel; Glutathione; Mercaptoethanol; Microscopy, Electron; Nucleic Acid Conformation; Oxidation-Reduction; Plasmids; Spectrum Analysis; Sulfhydryl Compounds | 1991 |
Affinity labeling of bovine adrenal 3 beta-hydroxysteroid dehydrogenase/steroid isomerase by 5'-[p-(fluorosulfonyl)benzoyl]adenosine.
Topics: 3-Hydroxysteroid Dehydrogenases; Adenosine; Adrenal Glands; Affinity Labels; Amino Acid Sequence; Animals; Binding Sites; Cattle; Cysteine; Dithiothreitol; Enzyme Activation; Molecular Sequence Data; NAD; Steroid Isomerases; Sulfhydryl Compounds | 1991 |
Lung surfactant protein SP-C from human, bovine, and canine sources contains palmityl cysteine thioester linkages.
Topics: Acylation; Amino Acid Sequence; Animals; Cattle; Chromatography, Gas; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Dogs; Humans; Molecular Sequence Data; Palmitic Acid; Palmitic Acids; Proteolipids; Pulmonary Surfactants; Spectrum Analysis; Sulfhydryl Compounds | 1991 |
Characterization of purified, reconstituted site-directed cysteine mutants of the lactose permease of Escherichia coli.
Topics: Amino Acid Sequence; Biological Transport; Cell Membrane; Cysteine; Dithiothreitol; Escherichia coli; Escherichia coli Proteins; Lactose; Membrane Potentials; Membrane Transport Modulators; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Naphthoquinones; Plasmids; Proteolipids; Symporters | 1991 |
Interaction of glutathione transferase from horse erythrocytes with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole.
Topics: 4-Chloro-7-nitrobenzofurazan; Animals; Cysteine; Dithiothreitol; Erythrocytes; Glutathione; Glutathione Transferase; Horses; Hydrogen-Ion Concentration; Lysine; Oxidation-Reduction; Spectrometry, Fluorescence; Spectrum Analysis; Sulfhydryl Reagents; Tetranitromethane; Tyrosine | 1991 |
In vitro DNA binding activity of Fos/Jun and BZLF1 but not C/EBP is affected by redox changes.
Topics: Amino Acid Sequence; Binding Sites; CCAAT-Enhancer-Binding Proteins; Cysteine; Dithiothreitol; DNA; DNA-Binding Proteins; Escherichia coli; Fungal Proteins; Glutathione Peroxidase; Hydrogen Peroxide; Leucine Zippers; Macromolecular Substances; Molecular Sequence Data; Nuclear Proteins; Oxidation-Reduction; Protein Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-jun; Recombinant Fusion Proteins; Recombinant Proteins; Saccharomyces cerevisiae Proteins; Trans-Activators; Transcription Factors; Viral Proteins | 1991 |
The effects of pantothenic acid, cysteine and dithiothreitol in intact, reperfused pig hearts.
Topics: Animals; Cysteine; Dithiothreitol; Fasting; Fatty Acids; Heart; Myocardial Contraction; Oxygen Consumption; Pantothenic Acid; Reperfusion Injury; Swine | 1991 |
Spectroscopic, immunochemical, and thermodynamic properties of carboxymethyl(Cys6, Cys127)-hen egg white lysozyme.
Topics: Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Hydrogen-Ion Concentration; Iodine Radioisotopes; Iodoacetates; Iodoacetic Acid; Methylation; Muramidase; Peptide Mapping; Protein Conformation; Thermodynamics; Trypsin | 1991 |
High-performance liquid chromatographic determination of urinary cysteine and cystine.
Topics: Chromatography, High Pressure Liquid; Cysteine; Cystinuria; Dithiothreitol; Humans; Indicators and Reagents; o-Phthalaldehyde; Oxidation-Reduction; Spectrometry, Fluorescence | 1991 |
Identification of bull protamine disulfides.
Topics: Amino Acid Sequence; Animals; Cattle; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Iodoacetates; Iodoacetic Acid; Male; Models, Molecular; Molecular Sequence Data; Peptide Fragments; Protamines; Protein Conformation; Spermatozoa; Tritium; Trypsin | 1991 |
Copper inhibits the protease from human immunodeficiency virus 1 by both cysteine-dependent and cysteine-independent mechanisms.
Topics: Cations; Copper; Cysteine; Dithiothreitol; HIV Protease; HIV Protease Inhibitors; In Vitro Techniques; Mercury; Oxygen | 1991 |
Properties of a novel nitric oxide-stimulated ADP-ribosyltransferase.
Topics: Adenosine Diphosphate Ribose; Blood Platelets; Cysteine; Cytosol; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Glutathione; Humans; Nitric Oxide; Nitroprusside; Poly(ADP-ribose) Polymerases | 1990 |
Kinetics of disulfide bond reduction in alpha-lactalbumin by dithiothreitol and molecular basis of superreactivity of the Cys6-Cys120 disulfide bond.
Topics: Chemical Phenomena; Chemistry, Physical; Circular Dichroism; Cysteine; Cystine; Dithiothreitol; Edetic Acid; Kinetics; Lactalbumin; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Spectrophotometry, Ultraviolet; Urea | 1990 |
The role of cysteine 41 in the enzymatic activities of the pertussis toxin S1 subunit as investigated by site-directed mutagenesis.
Topics: Adenosine Triphosphate; Base Sequence; Binding Sites; Catalysis; Cholic Acids; Cloning, Molecular; Codon; Cysteine; Dithiothreitol; Escherichia coli; Kinetics; Molecular Sequence Data; Mutation; NAD; NAD+ Nucleosidase; Pertussis Toxin; Poly(ADP-ribose) Polymerases; Structure-Activity Relationship; Sulfhydryl Compounds; Virulence Factors, Bordetella | 1990 |
Role of extracellular disulfide-bonded cysteines in the ligand binding function of the beta 2-adrenergic receptor.
Topics: Animals; Binding Sites; Binding, Competitive; Cricetinae; Cysteine; Disulfides; Dithiothreitol; Extracellular Space; Kinetics; Ligands; Mutation; Receptors, Adrenergic, beta | 1990 |
Effects of lead on K(+)-para-nitrophenyl phosphatase activity and protection by thiol reagents.
Topics: 4-Nitrophenylphosphatase; Animals; Binding, Competitive; Brain; Cysteine; Dithiothreitol; Enzyme Activation; Glutathione; Kinetics; Lead; Male; Phosphoric Monoester Hydrolases; Potassium; Rats; Rats, Inbred Strains; Sulfhydryl Compounds | 1990 |
Effects of dimethylsulfoxide and mercurial sulfhydryl reagents on water and solute permeability of rat kidney brush border membranes.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Cell Membrane Permeability; Cysteine; Dimethyl Sulfoxide; Dithiothreitol; Kidney; Kinetics; Mannitol; Mercury; Microvilli; Osmosis; Rats; Sodium Chloride; Sulfhydryl Reagents; Urea; Water | 1990 |
Affinity labeling of spinach phosphoribulokinase subsequent to S-methylation at Cys16.
Topics: Adenosine Triphosphate; Affinity Labels; Alkylation; Binding Sites; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Ethanolamines; Kinetics; Methylation; Peptide Mapping; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Plants; Protein Denaturation; Ribulosephosphates | 1990 |
Mechanism of formation of 6-amino-5-(N-methylformylamino)-1-methyluracil and 3,7-dimethyluric acid from theobromine in the rat in vitro: involvement of cytochrome P-450 and a cellular thiol.
Topics: Acetylcysteine; Animals; Cimetidine; Cysteine; Cytochrome P-450 Enzyme System; Dithiothreitol; Glutathione; Kinetics; Male; Metyrapone; Microsomes, Liver; Pyridines; Rats; Rats, Inbred Strains; Sulfhydryl Compounds; Theobromine; Uracil; Uric Acid | 1990 |
The mechanism of cysteine conjugate cytotoxicity in renal epithelial cells. Covalent binding leads to thiol depletion and lipid peroxidation.
Topics: Animals; Antioxidants; Carbon-Sulfur Lyases; Cell Line; Cell Survival; Cysteine; Deferoxamine; Dithiothreitol; Epithelium; In Vitro Techniques; Kidney; Lipid Peroxides; Lyases; Phenylenediamines; Rats; Sulfhydryl Compounds | 1990 |
Cellular recovery of glyceraldehyde-3-phosphate dehydrogenase activity and thiol status after exposure to hydroperoxides.
Topics: Cell Line; Cysteamine; Cysteine; Cystine; Dithiothreitol; Ethacrynic Acid; Ethylmaleimide; Glutathione; Glutathione Disulfide; Glyceraldehyde-3-Phosphate Dehydrogenases; Humans; Hydrogen Peroxide; Kinetics; Lung Neoplasms; Oxidation-Reduction; Peroxides; Sulfhydryl Compounds; tert-Butylhydroperoxide; Ultrasonics | 1990 |
Determination of reduced disulfide groups in monoclonal antibodies.
Topics: Antibodies, Monoclonal; Ascorbic Acid; Cysteine; Disulfides; Dithioerythritol; Dithiothreitol; Mercaptoethanol; Molybdenum; Ninhydrin; Nitroprusside; Oxidation-Reduction; Spectrophotometry; Tin; Tin Compounds | 1990 |
Determination of the disulfide array in sapecin, an antibacterial peptide of Sarcophaga peregrina (flesh fly).
Topics: Amino Acid Sequence; Animals; Anti-Infective Agents; Cells, Cultured; Chromatography, High Pressure Liquid; Cysteine; Diptera; Disulfides; Dithiothreitol; Insect Hormones; Insect Proteins; Molecular Sequence Data; Solubility; Thermolysin | 1990 |
Studies on in vivo inactivation of gramicidin S synthetase and its retardation.
Topics: 2,4-Dinitrophenol; Amino Acid Isomerases; Bacillus; Bacterial Proteins; Cysteine; Dinitrophenols; Dithiothreitol; Energy Metabolism; Fructose; Glucose; Glycerol; Gramicidin; Half-Life; Inositol; Multienzyme Complexes; Oxidation-Reduction; Peptide Synthases; Sorbitol; Time Factors | 1986 |
The nature of thiol-compounds which trap cuprous ion reductively liberated from bleomycin-Cu(II) in cells.
Topics: Alcohol Oxidoreductases; Animals; Bleomycin; Chromatography, High Pressure Liquid; Copper; Cysteine; Dithiothreitol; Female; Liver; Metallothionein; Rats; Sulfhydryl Compounds; Zinc | 1987 |
Double inhibition of L-threonine dehydratase by aminothiols.
Topics: Animals; Binding Sites; Binding, Competitive; Cysteamine; Cysteine; Dithiothreitol; Edetic Acid; Liver; Male; Pyridoxal Phosphate; Rats; Rats, Inbred Strains; Threonine Dehydratase | 1989 |
Studies on the kinetics of oxidation of 4-hydroxyanisole by tyrosinase.
Topics: Amino Acids; Anisoles; Basidiomycota; Catechol Oxidase; Cysteine; Dithiothreitol; Kinetics; Monophenol Monooxygenase; Oxidation-Reduction; Oxygen | 1989 |
The non-flavin redox center of the streptococcal NADH peroxidase. II. Evidence for a stabilized cysteine-sulfenic acid.
Topics: Amino Acid Sequence; Cysteine; Dithiothreitol; Enterococcus faecalis; Flavin-Adenine Dinucleotide; Hydrogen Peroxide; Isoelectric Focusing; Kinetics; Molecular Sequence Data; NAD; Neurotransmitter Agents; Nitrobenzoates; Oxidation-Reduction; Peptide Fragments; Peroxidases; Protein Denaturation; Spectrophotometry; Sulfhydryl Compounds; Trypsin | 1989 |
Functional cysteinyl residues in human placental aldose reductase.
Topics: Aldehyde Reductase; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Ethylmaleimide; Female; Humans; Kinetics; Models, Theoretical; Placenta; Pregnancy; Protein Binding; Substrate Specificity; Sugar Alcohol Dehydrogenases | 1989 |
Metabolism of leukotriene D4 by rat peritoneal mast cells.
Topics: Animals; beta-N-Acetylhexosaminidases; Chromatography, High Pressure Liquid; Chymases; Cysteine; Diazonium Compounds; Dipeptidases; Dithiothreitol; Histamine; Kinetics; L-Lactate Dehydrogenase; Leukotriene E4; Mast Cells; Peritoneum; Phenanthrolines; Rats; Rats, Inbred Strains; Serine Endopeptidases; SRS-A; Sulfanilic Acids | 1989 |
Neurotensin receptors on circular smooth muscle of canine small intestine: role of disulfide bridges.
Topics: Animals; Cell Membrane; Cysteine; Cystine; Disulfides; Dithiothreitol; Dogs; Intestine, Small; Kinetics; Muscle Contraction; Muscle, Smooth; Neurotensin; Receptors, Neurotensin; Receptors, Neurotransmitter | 1989 |
The role of sulphur peptide functions in free radical transfer: a pulse radiolysis study.
Topics: Chemical Phenomena; Chemistry; Cysteine; Disulfides; Dithiothreitol; Free Radicals; Glutathione; Methionine; Pulse Radiolysis; Spectrum Analysis; Sulfhydryl Compounds | 1989 |
Increase in intracellular cysteine after exposure to dithiothreitol: implications in radiobiology.
Topics: Animals; Cricetinae; Cysteine; Dithiothreitol; Fibroblasts; In Vitro Techniques; Radiation Tolerance; Radiation-Protective Agents; Stimulation, Chemical | 1989 |
Effect of thiols on lipid peroxidation in rat liver microsomes.
Topics: Acetylcysteine; Animals; Cysteamine; Cysteine; Dithiothreitol; Glutathione; Kinetics; Lipid Peroxidation; Male; Microsomes, Liver; Penicillamine; Rats; Rats, Inbred Strains; Structure-Activity Relationship; Sulfhydryl Compounds | 1989 |
Apparent sequence preference in cleavage of linear B-DNA by the Cu(II):thiol system.
Topics: 3-Mercaptopropionic Acid; Cations, Divalent; Copper; Cysteine; Deoxyribonuclease EcoRI; Dithiothreitol; DNA; DNA Damage; DNA, Superhelical; Glutathione; Nucleic Acid Conformation; Plasmids; Solutions; Sulfhydryl Compounds; Tyrosine | 1989 |
Evidence for the participation of Cys558 and Cys559 at the active site of mercuric reductase.
Topics: Binding Sites; Cysteine; Disulfides; Dithionite; Dithiothreitol; Escherichia coli; Kinetics; NADP; Oxidoreductases; Protein Binding; Spectrophotometry | 1989 |
Evidence for two different classes of redox-active cysteines in ribonucleotide reductase of Escherichia coli.
Topics: Allosteric Site; Base Sequence; Binding Sites; Cysteine; Dithiothreitol; Escherichia coli; Kinetics; Macromolecular Substances; Molecular Sequence Data; Mutation; Oligodeoxyribonucleotides; Oxidation-Reduction; Ribonucleotide Reductases | 1989 |
Effect of tributyltin on Escherichia coli: role of modifying factors.
Topics: Chlorides; Culture Media; Cysteine; Dithiothreitol; Escherichia coli; Glucose; Hydrogen-Ion Concentration; Succinates; Sulfates; Tin; Tin Compounds; Trialkyltin Compounds | 1989 |
Thiol proteases released in vitro by Fasciola hepatica.
Topics: Animals; Cysteine; Cysteine Endopeptidases; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Fasciola hepatica; In Vitro Techniques; Protease Inhibitors | 1989 |
Sulfhydryl groups of glucosamine-6-phosphate isomerase deaminase from Escherichia coli.
Topics: Allosteric Regulation; Chemical Phenomena; Chemistry; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Enzyme Reactivators; Escherichia coli; Glucose-6-Phosphate Isomerase; Hydrocarbons, Iodinated; Kinetics; Periodic Acid; Protein Denaturation; Structure-Activity Relationship; Sulfhydryl Compounds; Urea | 1987 |
Catalytic nonessentiality of an active-site cysteinyl residue of phosphoribulokinase.
Topics: Benzenesulfonates; Binding Sites; Cysteine; Dithiothreitol; Kinetics; Peptide Mapping; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Plants; Trypsin | 1988 |
Evidence for the existence of at least two different binding sites for 5HT-reuptake inhibitors within the 5HT-reuptake system from human platelets.
Topics: Arsenicals; Binding Sites; Blood Platelets; Carrier Proteins; Cysteine; Dithiothreitol; Ethylmaleimide; Humans; Imipramine; Neurotransmitter Uptake Inhibitors; Quinolines; Receptors, Drug; Receptors, Neurotransmitter; Serotonin | 1988 |
Cytoprotective effects of glycine and glutathione against hypoxic injury to renal tubules.
Topics: Adenosine Triphosphate; Animals; Cysteine; Dithiothreitol; Female; Glutamates; Glutamic Acid; Glutathione; Glycine; Kidney Diseases; Kidney Tubules, Proximal; Oxygen; Oxygen Consumption; Potassium; Rabbits | 1987 |
Analysis of biological thiols: determination of thiol components of disulfides and thioesters.
Topics: Animals; Bridged Bicyclo Compounds; Coenzyme A; Cysteine; Disulfides; Dithiothreitol; Esters; Ethylmaleimide; Glutathione; Hydroxylamine; Hydroxylamines; Male; Mitochondria, Liver; Neurospora crassa; Rats; Sulfhydryl Compounds | 1986 |
Effects of sulfhydryl agents on neuromuscular transmission in the presence or absence of cadmium.
Topics: Animals; Cadmium; Calcium; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; In Vitro Techniques; Mice; Neuromuscular Junction; Sulfhydryl Compounds; Synaptic Transmission | 1986 |
Chemical modification of alpha 2-adrenoceptors. Possible role for tyrosine in the ligand binding site.
Topics: Binding Sites; Cysteine; Dithiothreitol; Humans; Ligands; Receptors, Adrenergic, alpha; Tetranitromethane; Tyrosine | 1986 |
Thiol reagents are substrates for the ADP-ribosyltransferase activity of pertussis toxin.
Topics: Cysteine; Dithiothreitol; Kinetics; NAD; Pertussis Toxin; Poly(ADP-ribose) Polymerases; Substrate Specificity; Virulence Factors, Bordetella | 1988 |
Conformational features of bovine heart mitochondrial transhydrogenase.
Topics: Animals; Binding Sites; Cattle; Cross-Linking Reagents; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Ethylmaleimide; In Vitro Techniques; Mitochondria, Heart; NADH, NADPH Oxidoreductases; NADP; NADP Transhydrogenases; Protein Conformation; Sulfhydryl Reagents | 1988 |
Isoelectric focusing of basic proteins: the problem of oxidation of cysteines.
Topics: Adult; Alkylation; Cysteine; Dithiothreitol; Erythrocytes; Gels; Globins; Humans; Hydrogen-Ion Concentration; Infant, Newborn; Isoelectric Focusing; Macromolecular Substances; Mercaptoethanol; Oxidation-Reduction | 1988 |
Mitochondrial calcium release as induced by Hg2+.
Topics: Animals; Calcium; Cysteine; Dithiothreitol; Kidney; Kinetics; Membrane Potentials; Mercury; Mersalyl; Mitochondria; Rats; Submitochondrial Particles | 1988 |
Synergistic actions of disulfide-reducing agents on 1-methyladenine-induced oocyte maturation in starfish.
Topics: Adenine; Animals; Cysteine; Dimercaprol; Disulfides; Dithiothreitol; Drug Synergism; Oocytes; Oxidation-Reduction; Starfish | 1988 |
Primary structure of the light-dependent regulatory site of corn NADP-malate dehydrogenase.
Topics: Amino Acid Sequence; Carbocysteine; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Iodoacetates; Iodoacetic Acid; Light; Malate Dehydrogenase; Molecular Sequence Data; Oxidation-Reduction; Peptide Fragments; Plants; Thioredoxins; Trypsin; Zea mays | 1988 |
Thiol- and pH-modulated slow conformational changes and cooperativity of phenol-binding sites in phenol hydroxylase.
Topics: Binding Sites; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Kinetics; Mixed Function Oxygenases; Phenol; Phenols; Protein Binding; Protein Conformation; Trichosporon | 1988 |
Reaction of an exofacial sulfhydryl group on the erythrocyte hexose carrier with an impermeant maleimide. Relevance to the mechanism of hexose transport.
Topics: 3-O-Methylglucose; Affinity Labels; Binding, Competitive; Biological Transport; Cysteine; Cytochalasin B; Dithiothreitol; Drug Synergism; Erythrocytes; Humans; Maleimides; Maltose; Methylglucosides; Monosaccharide Transport Proteins; Phloretin; Photochemistry; Protein Conformation; Sulfhydryl Compounds | 1988 |
Disulfide bonds and the translocation of proteins across membranes.
Topics: Biological Transport; Cysteine; Disulfides; Dithiothreitol; Endoplasmic Reticulum; In Vitro Techniques; Membrane Proteins; Microsomes; Prolactin; Protein Conformation; Protein Precursors; Protein Processing, Post-Translational; Proteins | 1986 |
Modification of erythrocyte enzyme activities by persulfides and methanethiol: possible regulatory role.
Topics: Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Erythrocytes; Humans; Kinetics; Pyridoxal; Pyruvate Kinase; Sulfhydryl Compounds; Sulfides | 1987 |
Vitamin C and thiol reagents promote the in vitro growth of murine granulocyte/macrophage progenitor cells by neutralizing endogenous inhibitor(s).
Topics: Animals; Antioxidants; Ascorbic Acid; Cells, Cultured; Colony-Forming Units Assay; Colony-Stimulating Factors; Culture Media; Cysteine; Dithiothreitol; Free Radicals; Glutathione; Granulocytes; Hematopoiesis; Hematopoietic Stem Cells; Macrophages; Male; Mercaptoethanol; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Proteins | 1986 |
In vivo formation and stability of engineered disulfide bonds in subtilisin.
Topics: Autolysis; Bacillus; Computers; Cysteine; Disulfides; Dithiothreitol; Half-Life; Models, Molecular; Mutation; Subtilisins; Temperature; Urea | 1986 |
Recombinant DNA-derived forms of human alpha 1-proteinase inhibitor. Studies on the alanine 358 and cysteine 358 substituted mutants.
Topics: Alanine; alpha 1-Antitrypsin; Blood Proteins; Chemical Phenomena; Chemistry; Cysteine; Dithiothreitol; DNA, Recombinant; Endopeptidases; Humans; Kinetics; Macromolecular Substances; Mutation; Neutrophils; Pancreas; Pancreatic Elastase; Saccharomyces cerevisiae; Serine Endopeptidases; Structure-Activity Relationship | 1986 |
Intramolecular disulfide loop formation in a peptide containing two cysteines.
Topics: Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Glutathione; Guanidine; Guanidines; Iodoacetates; Kinetics; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Trypsin Inhibitor, Kunitz Soybean; Trypsin Inhibitors; Urea | 1987 |
Bromosulfophthalein abolishes glutathione-dependent protection against lipid peroxidation in rat liver mitochondria.
Topics: Animals; Ascorbic Acid; Cysteine; Dithiothreitol; Glutathione; Glutathione Peroxidase; Glutathione Transferase; Lipid Peroxides; Malondialdehyde; Mercaptoethanol; Mitochondria, Liver; Rats; Sulfobromophthalein; Vitamin E | 1987 |
N-hydroxy-2-acetylaminofluorene as effective inhibitor of aldehyde oxidase.
Topics: 2-Acetylaminofluorene; Aldehyde Oxidase; Aldehyde Oxidoreductases; Aminobiphenyl Compounds; Animals; Cysteine; Dithiothreitol; Fluorenes; Glutathione; Hydroxamic Acids; Hydroxyacetylaminofluorene; Kinetics; Male; Phenacetin; Rabbits; Serum Albumin, Bovine | 1986 |
Covalent binding of glutathione to hemoglobin. I. Inhibition of hemoglobin S polymerization.
Topics: Binding Sites; Cysteine; Dithiothreitol; Glutathione; Hemoglobin, Sickle; Hemoglobins; Humans; Kinetics; Macromolecular Substances; Protein Binding | 1986 |
The nonenzymatic oxidation of glutathione in the presence of plasmalike concentrations of disulfides and copper ions.
Topics: Copper; Cysteine; Dipeptides; Disulfides; Dithiothreitol; Glutathione; Glutathione Disulfide; Humans; Oxidation-Reduction | 1987 |
Functional role of cysteinyl residues in tryptophanase.
Topics: Catalysis; Chemical Phenomena; Chemistry; Cysteine; Dithiothreitol; Escherichia coli; Hydrogen-Ion Concentration; Kinetics; Lyases; Oxidation-Reduction; Specimen Handling; Thiocyanates; Tryptophanase | 1985 |
Redox interconversion of glutathione reductase from Escherichia coli. A study with pure enzyme and cell-free extracts.
Topics: Bacterial Proteins; Cysteine; Dithiothreitol; Enzyme Activation; Escherichia coli; Ferricyanides; Glutathione; Glutathione Reductase; Molecular Weight; NAD; NADP; Oxidation-Reduction; Oxygen | 1985 |
Involvement of zinc in the regulation of pHi, motility, and acrosome reactions in sea urchin sperm.
Topics: 2,2'-Dipyridyl; Acrosome; Ammonium Chloride; Animals; Cations; Cysteine; Dithiothreitol; Edetic Acid; Elements; Fertilization; Male; Phenanthrolines; Sea Urchins; Seawater; Sperm Motility; Spermatozoa; Zinc | 1985 |
Reaction of 5'-p-fluorosulfonylbenzoyl-1,N6-ethenoadenosine with histidine and cysteine residues in the active site of rabbit muscle pyruvate kinase.
Topics: Adenosine; Animals; Binding Sites; Cysteine; Dithiothreitol; Fluorescent Dyes; Histidine; Kinetics; Mathematics; Muscles; Pyruvate Kinase; Rabbits; Tetrazoles | 1985 |
FXIII induced gelation of human fibrinogen--an alternative thiol enhanced, thrombin independent pathway.
Topics: Calcium; Cysteine; Dithiothreitol; Fibrinogen; Gels; Humans; Mercaptoethanol; Nephelometry and Turbidimetry; Thrombin; Thromboplastin | 1985 |
Selective reduction of a disulphide bridge in hen ovotransferrin.
Topics: Alkylation; Animals; Chickens; Conalbumin; Cysteine; Disulfides; Dithiothreitol; Egg Proteins; Female; Iron; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Protein Denaturation; Urea | 1985 |
GSH-dependent inhibition of lipid peroxidation: properties of a potent cytosolic system which protects cell membranes.
Topics: Animals; Cell Membrane; Cysteine; Cytosol; Dithiothreitol; Glutathione; Iodoacetates; Iodoacetic Acid; Kinetics; Lipid Peroxides; Liver; Male; Mercaptoethanol; Oxygen Consumption; Rats; Rats, Inbred Strains | 1985 |
Tumor growth and disulfide reduction: possible dependence on protein-disulfide reductase.
Topics: Animals; Antigens, Neoplasm; Arsenic; Ascitic Fluid; Cell Survival; Cell-Free System; Cysteine; Disulfides; Dithiothreitol; Edetic Acid; Ethylmaleimide; Glutathione; Glutathione Reductase; Hot Temperature; Iodoacetates; Mice; NADH, NADPH Oxidoreductases; Neoplasm Transplantation; Neoplasms, Experimental; Oxidation-Reduction; Transplantation, Homologous | 1973 |
Mechanism of mercuric chloride resistance in microorganisms. 3. Purification and properties of a mercuric ion reducing enzyme from Escherichia coli bearing R factor.
Topics: Ammonium Sulfate; Apoproteins; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Thin Layer; Cysteine; D-Amino-Acid Oxidase; Dithiothreitol; Drug Resistance, Microbial; Escherichia coli; Extrachromosomal Inheritance; Flavin Mononucleotide; Flavin-Adenine Dinucleotide; Glutathione; Mercaptoethanol; Mercury; NADP; Spectrophotometry | 1974 |
Competitive radioprotection and radiosensitization in chemical systems.
Topics: Acetophenones; Ascorbic Acid; Binding, Competitive; Cyclic N-Oxides; Cysteamine; Cysteine; Dithiothreitol; DNA; Ethylmaleimide; Histamine; Metronidazole; NAD; NADP; Nitro Compounds; Nitrobenzenes; Nitrofurantoin; Nitrofurazone; Nitrophenols; Oxygen; Paraquat; Promethazine; Pyridinium Compounds; Quinones; Radiation Effects; Radiation-Protective Agents; Radiation-Sensitizing Agents; Tetrazolium Salts; Vitamin K | 1974 |
Reduction of adenosine 5'-phosphosulfate to cysteine in extracts from Chlorella and mutants blocked for sulfate reduction.
Topics: Adenosine Monophosphate; Carrier Proteins; Cell-Free System; Chlorella; Cysteine; Dithiothreitol; Ferredoxins; Glutathione; Kinetics; Models, Chemical; Multienzyme Complexes; Mutation; NADH, NADPH Oxidoreductases; Nucleotidyltransferases; Oxidation-Reduction; Protein Binding; Subcellular Fractions; Sulfides; Sulfites; Sulfur Radioisotopes; Sulfuric Acids; Sulfurtransferases; Thiosulfonic Acids | 1974 |
Mechanism of IgM polymerization.
Topics: Alkylation; Carbon Radioisotopes; Chemical Phenomena; Chemistry; Cysteine; Cystine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Humans; Immunoglobulin J-Chains; Immunoglobulin M; Iodoacetates; Oxidation-Reduction; Polymers; Tritium | 1974 |
Snake venom toxins. The reactivity of the disulphide bonds of Naja nivea toxin alpha.
Topics: Animals; Aspartic Acid; Binding Sites; Biological Assay; Carbon Radioisotopes; Chromatography, Gel; Chromatography, Ion Exchange; Chromatography, Paper; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Paper; Glycine; Immunodiffusion; Iodoacetates; Lethal Dose 50; Mice; Peptide Fragments; Phenylalanine; Protein Binding; Protein Conformation; Snakes; Spectrophotometry, Ultraviolet; Toxins, Biological; Tryptophan; Venoms | 1974 |
Stereospecific irreversible inhibition of histidine ammonia-lyase by L-cysteine.
Topics: Ammonia-Lyases; Animals; Binding Sites; Binding, Competitive; Carbon Radioisotopes; Cattle; Chromatography, Gel; Cysteine; Dithiothreitol; Edetic Acid; Erythrocytes; Histidine; Histidine Ammonia-Lyase; Homocysteine; Hydrogen-Ion Concentration; Isomerism; Kinetics; Macromolecular Substances; Mercaptoethanol; Oxygen; Protein Binding; Pseudomonas; Spectrophotometry, Ultraviolet; Structure-Activity Relationship; Sulfhydryl Compounds; Sulfur Radioisotopes; Superoxide Dismutase; Time Factors | 1974 |
Presence of a gelatin-specific proteinase and its latent form in human leucocytes.
Topics: Arthritis, Rheumatoid; Chloromercuribenzoates; Chromatography, Gel; Chromatography, Ion Exchange; Collagen; Cysteine; Dithiothreitol; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Gelatin; Humans; Hydrogen-Ion Concentration; Hydroxyproline; Iodoacetates; Isoflurophate; Kinetics; Leukocytes; Peptide Hydrolases; Structure-Activity Relationship; Synovial Fluid; Temperature; Trypsin Inhibitors; Viscosity | 1974 |
Uptake and efflux of sulfate in Neurospora crassa.
Topics: Biological Transport; Chloromercuribenzoates; Chromates; Cysteine; Dithiothreitol; Kinetics; Methionine; Mutation; Neurospora; Neurospora crassa; Selenium; Sulfates; Sulfites; Sulfur Radioisotopes; Thiosulfates; Time Factors | 1974 |
Modification of the catalytic properties of rabbit liver fructose diphosphatase by a particulate fraction from liver.
Topics: Adenosine Monophosphate; Adenosine Triphosphate; Ammonium Sulfate; Animals; Carrier Proteins; Catalysis; Chemical Precipitation; Chromatography, Ion Exchange; Coenzyme A; Cysteine; Disulfides; Dithiothreitol; Enzyme Activation; Filtration; Fructose-Bisphosphatase; Fructosephosphates; Homocystine; Hydrogen-Ion Concentration; Kinetics; Liver; Magnesium; Organophosphonates; Phenols; Phosphoric Monoester Hydrolases; Rabbits | 1971 |
Glycogen synthetase (UDP glucose: -1,4-glucan -4-glucosyl transferase (EC 2.4.1.11) I and D interconversion in the normal human epidermis.
Topics: Adenosine Triphosphate; Autoradiography; Carbon Isotopes; Cyclic AMP; Cysteine; Dithiothreitol; Glucose; Glucosyltransferases; Humans; Magnesium; Male; Mercaptoethanol; Skin | 1972 |
Purification of corneal collagenases.
Topics: Animals; Burns, Chemical; Calcium; Chromatography, Gel; Chromatography, Ion Exchange; Cornea; Cysteine; Dithiothreitol; Edetic Acid; Electrophoresis, Disc; Eye Burns; Immune Sera; Keratitis; Microbial Collagenase; Molecular Weight; Peptide Hydrolases; Rabbits; Sodium | 1972 |
[DNA polymerases in rat liver: thiol groups and enzyme activity].
Topics: Animals; Carcinoma, Hepatocellular; Chloromercuribenzoates; Cysteine; Dithiothreitol; DNA; DNA Nucleotidyltransferases; Imides; Liver; Liver Neoplasms; Liver Regeneration; Maleates; Maleimides; Mercaptoethanol; Rats | 1972 |
Periodate induced lymphocyte transformation. I. Inhibition by sulfhydryl reducing agents and sugars.
Topics: Alcohols; Carbohydrates; Cysteine; Dithiothreitol; Glutathione; Humans; Lymphocyte Activation; Lymphocytes; Oxidation-Reduction; Periodic Acid; Sulfhydryl Reagents; Sulfites; Thymidine; Tritium | 1972 |
Pharmacologic receptor activity of rabbit aorta. Effect of dithiothreitol and N-ethylmaleimide.
Topics: Amine Oxidase (Copper-Containing); Angiotensin II; Animals; Aorta; Cysteine; Cystine; Dithiothreitol; Erythritol; Ethylmaleimide; Histamine; In Vitro Techniques; Male; Methyltransferases; Muscle Contraction; Muscle, Smooth; Norepinephrine; Phentolamine; Potassium Chloride; Rabbits; Receptors, Drug; Serotonin | 1973 |
The effect of gold salts on the biosynthesis of uridine nucleotides in human granulocytes.
Topics: Amidohydrolases; Aspartate Carbamoyltransferase; Blood Proteins; Carbon Radioisotopes; Carboxy-Lyases; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Gold Sodium Thiomalate; Humans; In Vitro Techniques; Leukocytes; Mercaptoethanol; Orotic Acid; Oxidoreductases; Pentosyltransferases; Phosphotransferases; Sulfhydryl Compounds; Ultrasonics; Uracil Nucleotides | 1974 |
Virocidal cleavage of disulphide bonds within structural proteins of Sendai virus.
Topics: Acetamides; Animals; Carbon Radioisotopes; Centrifugation, Density Gradient; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Hemagglutination Inhibition Tests; Hemagglutinins, Viral; Hemolysin Proteins; Hemolysis; Immune Sera; Mercaptoethanol; Microscopy, Electron; Neuraminidase; Neutralization Tests; Parainfluenza Virus 1, Human; Peptides; Protein Denaturation; Rabbits; Viral Proteins | 1973 |
Comparison of the ribonucleotide with the canavanine reductase system.
Topics: Amino Acid Oxidoreductases; Animals; Canavanine; Carcinoma, Hepatocellular; Cell Division; Cells, Cultured; Cysteine; Dimercaprol; Dithiothreitol; Edetic Acid; Female; Hydroxylamines; Hydroxyurea; Iron; Liver Neoplasms; Neoplasm Transplantation; Neoplasms, Experimental; Oxidation-Reduction; Rats; Ribonucleotide Reductases; Succinates; Sulfhydryl Compounds; Thioctic Acid; Transferrin; Transplantation, Homologous | 1974 |
Disulphide groups of gonadotropin receptors are essential for specific binding of human chorionic gonadotropin.
Topics: Animals; Binding Sites; Centrifugation, Density Gradient; Chorionic Gonadotropin; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Female; Humans; Iodine Radioisotopes; Male; Methylene Blue; Oxygen; Protein Binding; Receptors, Cell Surface; Testis | 1974 |
Functional groups of diphosphopyridine nucleotide-linked isocitrate dehydrogenase from bovine heart. Studies of cysteine residues.
Topics: Amino Acids; Animals; Benzoates; Binding Sites; Cattle; Chloromercuribenzoates; Cyanides; Cysteine; Dithiothreitol; Ethylmaleimide; Hydrogen-Ion Concentration; Iodoacetates; Isocitrate Dehydrogenase; Kinetics; Mercaptoethanol; Molecular Weight; Myocardium; NAD; Nitro Compounds; Oxidation-Reduction; Protein Binding; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Temperature; Thiosulfates; Time Factors | 1974 |
Inhibition of membrane adenosine triphosphatase by alpha-tocopherol and its derivatives.
Topics: Acetates; Acetylcholinesterase; Adenosine Triphosphatases; Animals; Brain; Butylated Hydroxytoluene; Calcium; Cysteine; Dithiothreitol; Erythrocytes; Glucose-6-Phosphatase; Humans; Kinetics; Liver; Membranes; Microsomes; Muscles; Nucleotidases; Ouabain; Potassium; Quinones; Rabbits; Sodium; Swine; Vitamin E | 1974 |
On site heterogeneity in sturgeon muscle GPDH: a kinetic approach.
Topics: Animals; Apoenzymes; Benzoates; Binding Sites; Computers; Cysteine; Disulfides; Dithiothreitol; Fishes; Glyceraldehyde-3-Phosphate Dehydrogenases; Kinetics; Macromolecular Substances; Models, Chemical; Muscles; NAD; Nitro Compounds; Spectrophotometry | 1974 |
Conversion of UDP-D-glucuronic acid to UDP-D-apiose and UDP-D-xylose by an enzyme isolated from Lemna minor.
Topics: Carbon Radioisotopes; Carboxy-Lyases; Centrifugation, Density Gradient; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Ion Exchange; Chromatography, Paper; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Glucuronates; Hydroxyapatites; Isotope Labeling; Kinetics; NAD; Pentoses; Seaweed; Structure-Activity Relationship; Time Factors; Tritium; Uridine Diphosphate Sugars; Xylose | 1974 |
Automation of a kinetic spectrophotometric assay for ATP: creatine phosphotransferase activity suitable for human serum and muscle extracts.
Topics: Autoanalysis; Creatine Kinase; Cysteine; Dithiothreitol; Enzyme Activation; Female; Glucosephosphate Dehydrogenase; Glutathione; Glutathione Reductase; Humans; Male; Mercaptoethanol; Methods; Muscles; NADP; Spectrophotometry; Ultraviolet Rays | 1972 |
Fat metabolism in higher plants. The nonenzymatic acylation of dithiothreitol by acyl coenzyme A.
Topics: Acetates; Carbon Radioisotopes; Chloroplasts; Chromatography, Gas; Chromatography, Thin Layer; Citrate (si)-Synthase; Coenzyme A; Cysteine; Darkness; Dithiothreitol; Drug Stability; Fatty Acids; Glutathione; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Mass Spectrometry; Mathematics; Mercaptoethanol; Oxidation-Reduction; Plants | 1974 |
Sulfhydryl compounds under controlled gas in culture of Schistosoma mansoni sporocysts.
Topics: Animals; Carbon Dioxide; Culture Media; Cysteine; Dithiothreitol; Glutathione; Oxygen; Schistosoma mansoni; Snails; Spores; Sulfhydryl Compounds | 1974 |
Amino acid sequence of a peptide containing the active cysteine residue of histidine ammonia-lyase.
Topics: Amino Acid Sequence; Amino Acids; Ammonia-Lyases; Autoradiography; Carbon Radioisotopes; Cell Fractionation; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Paper; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Histidine Ammonia-Lyase; Iodoacetates; Kinetics; Methylation; Peptides; Pseudomonas; Sulfhydryl Compounds; Thermolysin; Trypsin | 1974 |
Isolation and partial characterization of procollagen fractions produced by a clonal strain of calf dermatosparactic cells.
Topics: Amino Acids; Animals; Cattle; Cattle Diseases; Chromatography, DEAE-Cellulose; Clone Cells; Collagen; Collagen Diseases; Culture Media; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Fibroblasts; Microscopy, Electron; Molecular Weight; Peptides; Protein Precursors; Skin; Spectrum Analysis; Tritium; Tryptophan | 1974 |
Enzymic synthesis of steroid sulphates. X. Isolation of oestrogen sulphotransferase from bovine placenta and comparison of its properties with adrenal oestrogen sulphotransferase.
Topics: Adenine Nucleotides; Adrenal Cortex; Adrenal Glands; Amino Acids; Animals; Cattle; Chromatography, DEAE-Cellulose; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Estrogens; Estrone; Female; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; Mercaptoethanol; Molecular Weight; Organ Specificity; Placenta; Pregnancy; Sulfurtransferases; Time Factors; Ultracentrifugation | 1974 |
Structure and function of chloroplast proteins. XXII. Dissociation and reconstitution of spinach leaf ribulose-1,5-diphosphate carboxylase.
Topics: Carboxy-Lyases; Centrifugation, Density Gradient; Chromatography, Gel; Cysteine; Dithiothreitol; Electrophoresis, Disc; Glutathione; Glyceric Acids; Hydrogen-Ion Concentration; Mercaptoethanol; Mercuribenzoates; Pentosephosphates; Plants; Protein Conformation; Time Factors; Ultracentrifugation | 1974 |
Microbial metabolism of the pyridine ring. Metabolism of 2- and 3-hydroxypyridines by the maleamate pathway in Achromobacter sp.
Topics: Alcaligenes; Amides; Ammonia; Ammonium Sulfate; Centrifugation; Chelating Agents; Chromatography, Paper; Cysteine; Dithiothreitol; Fumarates; Glutathione; Iron; Maleates; Oxygenases; Pyridines | 1974 |
Structure and properties of arginase from the polychaete annelid Pista pacifica Berkeley.
Topics: Arginase; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Kinetics; Manganese; Mercaptoethanol; Polychaeta; Temperature | 1974 |
Enzymic synthesis of steroid sulphates. IX. Physical and chemical properties of purified oestrogen sulphotransferase from bovine adrenal glands, the nature of its isoenzymic forms and a proposed model to explain its wave-like kinetics.
Topics: Adenine Nucleotides; Adrenal Glands; Amino Acids; Anilino Naphthalenesulfonates; Animals; Cattle; Chemical Phenomena; Chemistry, Physical; Chromatography, DEAE-Cellulose; Chromatography, Ion Exchange; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Estrogens; Estrone; Hydroxymercuribenzoates; Iodoacetates; Isoenzymes; Kinetics; Maleates; Molecular Weight; Protein Conformation; Sulfur Radioisotopes; Sulfurtransferases; Time Factors; Ultracentrifugation | 1974 |
A chromatographic and histochemical study of nonspecific esterases in human carious dentine.
Topics: Bile Acids and Salts; Chromatography, DEAE-Cellulose; Chromatography, Gas; Cysteine; Dental Caries; Dentin; Dithiothreitol; Esterases; Fatty Acids; Histocytochemistry; Humans; Hydrogen-Ion Concentration; Mercury; Naphthols; Ultrasonics | 1972 |
Alanine and aspartate aminotransferases in sound and carious human dentine.
Topics: Alanine Transaminase; Aspartate Aminotransferases; Chromatography, DEAE-Cellulose; Cysteine; Dental Caries; Dentin; Dithiothreitol; Humans; Hydrogen-Ion Concentration; Mercury; Temperature | 1972 |
Chemical modulation of cell surfaces by sulfhydryl compounds: effect on C3b receptors.
Topics: ABO Blood-Group System; Acetamides; Animals; beta-Aminoethyl Isothiourea; Binding Sites, Antibody; Cell Line; Cell Membrane; Cell Separation; Complement System Proteins; Concanavalin A; Cysteine; Cytotoxicity Tests, Immunologic; Dithiothreitol; Dose-Response Relationship, Drug; Erythrocytes; Glutathione; Hemagglutination Tests; Hemoglobinuria, Paroxysmal; Humans; Hydrogen-Ion Concentration; Hypersplenism; Immune Adherence Reaction; Isoantibodies; Leukemia, Lymphoid; Lupus Erythematosus, Systemic; Lymphocytes; Mercaptoethanol; Microscopy, Electron, Scanning; Sheep; Sulfhydryl Compounds | 1974 |
Properties of amphibian renal arginase. I. The effect of dialysis and sulphydryl compounds.
Topics: Acetone; Animals; Anura; Arginase; Cattle; Chemical Precipitation; Chloromercuribenzoates; Chromatography, Ion Exchange; Cysteine; Dialysis; Dithiothreitol; Enzyme Activation; Glutathione; Kidney; Liver; Mercaptoethanol; Microsomes; Microsomes, Liver; Rana catesbeiana; Rana pipiens; Rats; Species Specificity | 1972 |
Potentiometric and colorimetric methods for the assay of 2',3'-cyclic nucleotide 3'-phosphohydrolase.
Topics: Alkaline Phosphatase; Animals; Buffers; Cerebral Cortex; Colorimetry; Cysteine; Dithiothreitol; Escherichia coli; Hydrogen-Ion Concentration; Mathematics; Mercaptoethanol; Methods; Nucleotides; Phosphates; Potentiometry; Proteins; Swine; Time Factors | 1973 |
Purification and properties of an avian carbonic anhydrase from the erythrocytes of Gallus domesticus.
Topics: Acetazolamide; Amino Acids; Animals; Carbonic Anhydrases; Chickens; Chromatography, Gel; Chromatography, Ion Exchange; Circular Dichroism; Cysteine; Dithiothreitol; Drug Stability; Electrophoresis; Erythrocytes; Esterases; Female; Kinetics; Mercaptoethanol; Optical Rotatory Dispersion; Oxidation-Reduction; Species Specificity; Spectrophotometry; Ultracentrifugation; Ultraviolet Rays; Zinc | 1972 |
Effect of copper on urate oxidase.
Topics: Acetone; Ammonia; Animals; Catalysis; Cattle; Copper; Cysteine; Dithiothreitol; Edetic Acid; Hot Temperature; Hydrogen-Ion Concentration; Kidney; Mitochondria; Osmolar Concentration; Oxidation-Reduction; Protein Conformation; Spectrophotometry; Sulfates; Time Factors; Tromethamine; Urate Oxidase | 1972 |
Immune responses in vitro. V. Role of mercaptoethanol in the mixed-leukocyte reaction.
Topics: Animals; Cattle; Cell Survival; Cysteine; Dithiothreitol; Ethanol; Fetus; Glutathione; Histocompatibility Testing; Kinetics; Lymphocyte Activation; Lymphocytes; Male; Mercaptoethanol; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Serum Albumin, Bovine; Spleen; Thymidine; Time Factors; Tritium | 1972 |
The effect of thiols on oestradiol binding by components of human malignant breast tumours.
Topics: Binding Sites; Breast Neoplasms; Cysteine; Dithiothreitol; Estradiol; Female; Humans; Mercaptoethanol; Sulfhydryl Compounds | 1972 |
Selective cysteine modification in glyceraldehyde-3-phosphate dehydrogenase.
Topics: Acetone; Alkylation; Amino Acids; Animals; Benzoates; Binding Sites; Bromine; Carbon Isotopes; Chemical Phenomena; Chemistry; Chromatography, Paper; Cysteine; Dithiothreitol; Electrophoresis, Paper; Enzyme Activation; Ethylmaleimide; Glyceraldehyde-3-Phosphate Dehydrogenases; Iodine; Iodoacetates; Mercaptoethanol; Muscles; Papain; Peptides; Rabbits; Thiosulfates; Trypsin | 1972 |
Protein determination by Lowry's method in the presence of sulfhydryl reagents.
Topics: Animals; Cattle; Copper; Cysteine; Dithiothreitol; Glutathione; Hydrogen Peroxide; Kinetics; Mercaptoethanol; Methods; Phenols; Proteins; Serum Albumin, Bovine; Spectrophotometry; Temperature | 1972 |
Beef-liver 5-aminolevulinic acid dehydratase. Purification and properties.
Topics: Amino Acids; Animals; Cadmium; Cattle; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Chromatography, Gel; Chromatography, Ion Exchange; Cysteine; Dithiothreitol; Electrophoresis, Disc; Guanidines; Hydro-Lyases; Hydrogen-Ion Concentration; Hydrolysis; Lead; Levulinic Acids; Liver; Macromolecular Substances; Mercaptoethanol; Molecular Weight; Sodium Dodecyl Sulfate; Ultracentrifugation; Zinc | 1972 |
[Experimental studies on the chemical dissolution of cystine stones].
Topics: Animals; Cysteamine; Cysteine; Cystine; Disulfides; Dithiothreitol; Glutathione; Glycine; Isomerism; Kidney Calculi; Lethal Dose 50; Mercaptopurine; Mice; Penicillamine; Rabbits; Rats; Solubility; Spectrophotometry, Infrared; Sulfhydryl Compounds; Water | 1972 |
Collagenolytic-cathepsin and acid-proteinase activities in the rat uterus during post partum involution.
Topics: Animals; Cathepsins; Chloromercuribenzoates; Collagen; Cysteine; Dithiothreitol; Enzyme Activation; Female; Hemoglobins; Hydrogen-Ion Concentration; Peptide Hydrolases; Postpartum Period; Pregnancy; Rats; Time Factors; Uterus | 1973 |
Influence of thiols, ATP and CoA on protein breakdown by subcellular fractions from rat liver.
Topics: Adenosine Triphosphate; Animals; Carbon Isotopes; Coenzyme A; Cysteine; Dithiothreitol; Glutathione; Hydrogen-Ion Concentration; In Vitro Techniques; Liver; Lysosomes; Mitochondria, Liver; Phenylalanine; Rats; Serum Albumin; Subcellular Fractions; Sulfhydryl Compounds; Surface-Active Agents | 1973 |
Effect of reducing agents on the structure and the function of the third component of guinea pig complement (C3). I. Inhibition of specific binding activity of guinea pig complement C3.
Topics: Acetamides; Alkylation; Animals; Cell Membrane; Complement System Proteins; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Guinea Pigs; Hemolysis; Immune Adherence Reaction; Mercaptoethanol; Oxidation-Reduction; Protein Binding | 1973 |
Influence of cadmium ions on the transmembrane potential and contractility of isolated rabbit left atria.
Topics: Action Potentials; Animals; Cadmium; Calcium; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Electric Stimulation; Glutathione; Heart Atria; In Vitro Techniques; Membrane Potentials; Muscle Contraction; Rabbits; Time Factors | 1973 |
Inhibition of brain adenylate cyclase by ethacrynic acid and dithiobisnitrobenzoic acid.
Topics: Adenylyl Cyclase Inhibitors; Animals; Benzoates; Brain; Cerebral Cortex; Cysteine; Disulfides; Dithiothreitol; Ethacrynic Acid; Female; Mice; Nitro Compounds | 1973 |
Induction of starfish oocyte maturation by disulfide-reducing agents.
Topics: Adenine; Amides; Animals; Chloromercuribenzoates; Cysteine; Dimercaprol; Disulfides; Dithiothreitol; Ethylmaleimide; Female; Fertilization; Glutathione; Insemination; Iodides; Iodoacetates; Iodobenzoates; Larva; Male; Ovum; Peroxides; Starfish; Time Factors | 1973 |
Agglutinating activities of the subunits of one IgM cold agglutinin treated with various reducing agents.
Topics: Alkylation; Antibodies; Chromatography, Gel; Cold Temperature; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Hemagglutination; Immunoglobulin Fragments; Immunoglobulin M; Mercaptoethanol; Mercaptoethylamines; Oxidation-Reduction; Peptides; Proteins | 1973 |
Methionine metabolism in mammals: synthesis of S-adenosylhomocysteine in rat tissues.
Topics: Adenosine; Animals; Carbon Radioisotopes; Chromatography, Ion Exchange; Chromatography, Paper; Cysteine; Dithiothreitol; Glucagon; Glutathione; Growth Hormone; Homocysteine; Hydrocortisone; Isotope Labeling; Liver; Male; Methionine; Organ Specificity; Rats; S-Adenosylmethionine; Thyroxine; Tritium | 1973 |
[Purification and properties of L-cysteinsulfinic decarboxylase from horse kidneys].
Topics: Animals; Carboxy-Lyases; Chromatography; Cysteamine; Cysteine; Dithiothreitol; Horses; Hydrogen-Ion Concentration; Kidney; Mercaptoethanol; Methods; Sulfhydryl Compounds; Sulfinic Acids; Taurine | 1973 |
Cytochrome P-450cam. II. Interconversion with P-420.
Topics: Acetone; Camphor; Carbon Monoxide; Chloromercuribenzoates; Cysteine; Cytochrome P-450 Enzyme Inhibitors; Cytochrome P-450 Enzyme System; Deoxycholic Acid; Dithiothreitol; Homocysteine; Mercaptoethanol; Oxidation-Reduction; Pseudomonas; Spectrophotometry; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds; Thioglycolates; Urea | 1974 |
A microassay for arylamidase activity.
Topics: Aminopeptidases; Animals; Cerebral Cortex; Cysteine; Dithiothreitol; Enzyme Activation; Evaluation Studies as Topic; Freeze Drying; Hydrogen-Ion Concentration; Hydroxymercuribenzoates; Male; Phenylalanine; Pituitary Gland, Posterior; Rats; Spectrometry, Fluorescence; Temperature | 1974 |
Studies on the disulfide bonds of glycoprotein hormones. Locations in the alpha chain based on partial reductions and formation of 14C-labeled S-carboxymethyl derivatives.
Topics: Acetates; Alkylation; Amino Acid Sequence; Amino Acids; Animals; Carbon Radioisotopes; Cattle; Chromatography, Gel; Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Electrophoresis; Glycopeptides; Iodoacetates; Luteinizing Hormone; Molecular Conformation; Oxidation-Reduction; Thermolysin; Thyrotropin; Time Factors; Trypsin | 1974 |
Phosphate-mediated alteration of the Microsporum gypseum germination protease specificity for substrate: enhanced keratinase activity.
Topics: Animals; Caseins; Chickens; Chromatography; Cysteine; Dithiothreitol; Endopeptidases; Enzyme Induction; Esterases; Feathers; Guinea Pigs; Hair; Horses; Humans; Hydrogen-Ion Concentration; Keratins; Microsporum; Models, Biological; Molecular Weight; Multienzyme Complexes; Phosphates; Spores, Fungal; Sulfites | 1974 |
The use of dithiothreitol in in vitro studies on rumen microflora.
Topics: Animals; Bacteria; Bacteriological Techniques; Chemical Phenomena; Chemistry; Culture Media; Cysteine; Dithiothreitol; Hydrochloric Acid; Rumen; Sheep; Sodium; Sulfites | 1971 |
Studies on the reductive cleavage of canavanine and canavaninosuccinic acid.
Topics: Adenosine Triphosphate; Anaerobiosis; Animals; Bicarbonates; Canavanine; Carrier Proteins; Chromatography, DEAE-Cellulose; Chromatography, Gel; Cobalt; Cyanides; Cysteine; Deoxyribonucleotides; Dimercaprol; Dithiothreitol; Dogs; Edetic Acid; Electrophoresis; Guanidines; Haplorhini; Homoserine; Humans; Hydrogen-Ion Concentration; Iron; Kinetics; Liver; Oxidation-Reduction; Papio; Quaternary Ammonium Compounds; Serum Albumin; Sodium; Spectrophotometry; Succinates; Thioctic Acid; Transferrin; Ultraviolet Rays | 1971 |
Properties of rat bone inorganic pyrophosphatase studied by means of 32 P-radioassay.
Topics: Animals; Ascorbic Acid; Barium; Bone and Bones; Chloromercuribenzoates; Cobalt; Cysteine; Dithiothreitol; Edetic Acid; Hydrogen-Ion Concentration; Iodoacetates; Iron; Magnesium; Manganese; Mercury; Phosphorus Isotopes; Pyrophosphatases; Rats; Tibia; Zinc | 1971 |
Creatine phosphokinase isoenzymes in human tissue with special reference to brain extract.
Topics: Brain; Creatine Kinase; Cysteine; Dithiothreitol; Electrophoresis; Enzyme Activation; Glutathione; Humans; Isoenzymes; Muscles; Muscular Dystrophies; Myocardium | 1972 |
Reactions at the 3' terminus of transfer ribonucleic acid. II. Purification and physical and chemical properties of rabbit liver transfer ribonucleic acid nucleotidyltransferase.
Topics: Amino Acids; Animals; Chromatography; Chromatography, DEAE-Cellulose; Chromatography, Gel; Chromatography, Ion Exchange; Cysteine; Detergents; Dithiothreitol; Drug Stability; Electrophoresis; Hot Temperature; Hydroxyapatites; Liver; Macromolecular Substances; Molecular Weight; Protein Binding; Rabbits; RNA Nucleotidyltransferases; RNA, Transfer; Sulfhydryl Reagents; Sulfuric Acids; Ultracentrifugation | 1972 |
Pseudoisoenzymes of rabbit muscle phosphoglucose isomerase.
Topics: Amino Acids; Animals; Chromatography, Ion Exchange; Crystallization; Cysteine; Dithiothreitol; Evaluation Studies as Topic; Glucose-6-Phosphate Isomerase; Hydrogen-Ion Concentration; Isoelectric Focusing; Isoenzymes; Mercuribenzoates; Muscles; Oxidation-Reduction; Rabbits; Sulfhydryl Compounds | 1972 |
Concerning the similarities between three alkaline phosphatases of human foetal parietal bones.
Topics: Acrylamides; Alkaline Phosphatase; Calcium; Cysteine; Dithiothreitol; Electrophoresis; Fetus; Gestational Age; Humans; Hydrolysis; Ions; Magnesium; Parietal Bone; Quaternary Ammonium Compounds | 1972 |
Activation of serum creatine kinase by dithiothreitol.
Topics: Acetates; Clinical Enzyme Tests; Creatine Kinase; Cysteine; Dithiothreitol; Enzyme Activation; Female; Glutathione; Humans; Kinetics; Male; Mercaptoethanol; Muscular Diseases; Sex Factors; Spectrophotometry; Sulfhydryl Reagents; Thermodynamics | 1972 |
Chemical characterization of avian infectious bronchitis virus receptor sites.
Topics: Animals; Ascorbic Acid; Binding Sites; Bird Diseases; Bronchitis; Cells, Cultured; Chick Embryo; Cysteine; Dithiothreitol; Glutathione; Hydroxymercuribenzoates; Kidney; Mercaptoethanol; Neuraminic Acids; Neuraminidase; Newcastle disease virus; Oxidation-Reduction; RNA Viruses; Salicylates; Sulfhydryl Compounds; Swine; Virus Diseases | 1972 |
Stability constant for the zinc-dithiothreitol complex.
Topics: Animals; Chelating Agents; Cysteine; Dithiothreitol; Drug Stability; Enzyme Activation; Histidine; Liver; Lyases; Mercaptoethanol; Methods; Rats; Zinc | 1972 |
p-Toluenethiol as an initiator of autolysis in bakers' yeast.
Topics: Autolysis; Cell Wall; Cresols; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Methods; Saccharomyces; Sucrase; Time Factors; Toluene | 1972 |
Role of tryptophan in dihydrofolate reductase.
Topics: Bromine; Catalysis; Circular Dichroism; Cysteine; Dithiothreitol; Enzyme Activation; Folic Acid Antagonists; Mathematics; Oxidation-Reduction; Spectrophotometry, Ultraviolet; Streptococcus; Succinimides; Tetrahydrofolate Dehydrogenase; Tryptophan; Ultraviolet Rays | 1972 |
The unique reassociation of human IgA2 immunoglobulins from dimer subunits.
Topics: Alkylation; Amides; Chromatography, Gel; Cysteine; Disulfides; Dithiothreitol; Electrophoresis; Electrophoresis, Polyacrylamide Gel; Humans; Immunoelectrophoresis; Immunoglobulin A; Immunoglobulin Fragments; Iodine Isotopes; Iodoacetates; Molecular Conformation; Myeloma Proteins; Oxidation-Reduction | 1972 |
Effects on lethality of toxins in venom from the scorpion Centruroides sculpturatus by group specific reagents.
Topics: Amino Acids; Animals; Antitoxins; Azo Compounds; Benzene Derivatives; Chloromercuribenzoates; Cysteamine; Cysteine; Dithiothreitol; Iodoacetates; Iodobenzoates; Methyl Ethers; Mice; Proteins; Scorpions; Sulfhydryl Reagents; Sulfonic Acids; Thioglycolates; Toxins, Biological; Urea; Venoms | 1972 |
Evidence on erythrocyte aminopeptidase B.
Topics: Aminopeptidases; Animals; Arginine; Borates; Chloromercuribenzoates; Cysteine; Dithiothreitol; Drug Stability; Electrophoresis, Disc; Enzyme Activation; Erythrocytes; Ethylmaleimide; Hot Temperature; Isoenzymes; Kinetics; Liver; Lysine; Mathematics; Methylene Blue; Naphthalenes; Phosphates; Photolysis; Radiation Effects; Rats; Sodium Chloride | 1971 |
An immunoglobulin light chain of subgroup III-1 of lambda type with a free sulfhydryl group.
Topics: Amino Acid Sequence; Ammonium Sulfate; Bence Jones Protein; Chemical Precipitation; Chromatography, Gel; Cysteine; Dansyl Compounds; Dithiothreitol; Electrophoresis; Guanidines; Humans; Immunoglobulin G; Immunoglobulins; Iodine Isotopes; Iodoacetates; Methylation; Multiple Myeloma; Myeloma Proteins; Oxidation-Reduction; Peptides; Protein Denaturation; Sulfhydryl Compounds; Tryptophan; Tyrosine; Urea; Valine | 1971 |
Assembly of immunoglobulin M. Blocked thiol groups of intracellular 7S subunits.
Topics: Alkylating Agents; Animals; Centrifugation, Density Gradient; Chromatography, Gel; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Disc; Immunoglobulin G; Immunoglobulins; In Vitro Techniques; Iodoacetates; Leucine; Mice; Multiple Myeloma; Neoplasms, Experimental; Oxidation-Reduction; Polymers; Tritium | 1971 |
Comparative mucolytic studies on dithiothreitol, N-acetyl-cysteine and L-cysteine on human respiratory mucus in vitro and their effects on the rate of flow of mucus in the exposed trachea of the rat on topical application.
Topics: Acetylcysteine; Animals; Bronchi; Cysteine; Dithiothreitol; Humans; In Vitro Techniques; Irritants; Models, Biological; Mucus; Rats; Rheology; Stimulation, Chemical; Trachea; Viscosity | 1971 |
DDT-dehydrochlorinase. II. Subunits, sulfhydryl groups, and chemical composition.
Topics: Amino Acids; Animals; Centrifugation, Density Gradient; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Chromatography, Gel; Chromatography, Ion Exchange; Chromatography, Paper; Cysteine; DDT; Dithiothreitol; Electrophoresis, Disc; Electrophoresis, Paper; Enzyme Activation; Glutathione; Houseflies; Kinetics; Lipoproteins; Lyases; Macromolecular Substances; Mercaptoethanol; Molecular Weight; Peptides; Trypsin | 1971 |
On the process of induction of adenosine triphosphate hydrolysis by chloroplasts with cysteine as activating thiol.
Topics: Adenosine Triphosphate; Ammonium Chloride; Chloroplasts; Cysteine; Dithiothreitol; Diuron; Electron Transport; Flavin Mononucleotide; Hydrolysis; Kinetics; Light; Magnesium; Oxygen; Phenazines; Plants; Radiation Effects; Sulfites | 1971 |
Kinetic aspects of the reaggregation of a human immunoglobulin M.
Topics: Alkylation; Amino Acids; Borohydrides; Complement Fixation Tests; Cysteine; Disulfides; Dithiothreitol; Humans; Hydrogen-Ion Concentration; Imidazoles; Immunochemistry; Immunoglobulins; Kinetics; Mercaptoethanol; Models, Chemical; Oxidation-Reduction; Sulfhydryl Compounds; Ultracentrifugation | 1971 |
Bacterial persistence in streptococcal endocarditis due to thiol-requiring mutants.
Topics: Adolescent; Adult; Culture Media; Cysteine; Dithiothreitol; Endocarditis, Bacterial; Female; Glutathione; Humans; L Forms; Male; Middle Aged; Penicillin G; Penicillin Resistance; Streptococcal Infections; Streptococcus; Streptomycin; Thioglycolates | 1971 |
Enzymatic degradation of L-cysteine in human dental plaque.
Topics: Aluminum; Calcium; Chromatography; Chromium; Coenzymes; Copper; Cyanides; Cysteine; Cystine; Dental Plaque; Dithiothreitol; Humans; Hydrogen-Ion Concentration; Iron; Keto Acids; Mercaptoethanol; Oxidoreductases; Temperature; Time Factors; Ultracentrifugation | 1971 |
Isolation of non-identical polypeptide chains of -crystallin.
Topics: Alkylation; Amino Acids; Animals; Cattle; Chemical Precipitation; Chromatography, Gel; Chromatography, Ion Exchange; Crystallins; Cysteine; Detergents; Disulfides; Dithiothreitol; Electrophoresis; Ethanol; Formates; Hydrogen-Ion Concentration; Hydrolysis; Iodoacetates; Macromolecular Substances; Mercaptoethanol; Oxidation-Reduction; Peptides; Sulfhydryl Compounds; Sulfuric Acids; Ultracentrifugation; Urea | 1969 |
Factors influencing the oxidation of cysteamine and other thiols: implications for hyperthermic sensitization and radiation protection.
Topics: Catalase; Culture Media; Cysteamine; Cysteine; Dithiothreitol; Ditiocarb; Edetic Acid; Glutathione; Hot Temperature; Oxidation-Reduction; Pentetic Acid; Peroxides; Radiation-Protective Agents; Superoxide Dismutase | 1984 |
Possible involvement of S-nitrosothiols in the activation of guanylate cyclase by nitroso compounds.
Topics: Acetylcysteine; Animals; Cattle; Coronary Vessels; Cysteine; Dithiothreitol; Enzyme Activation; Ferricyanides; Glutathione; Guanylate Cyclase; Kinetics; Liver; Methemoglobin; Myoglobin; Nitroso Compounds; Protein Binding; Rats; S-Nitrosoglutathione; S-Nitrosothiols; Sulfhydryl Compounds; Thioglucosides; Valine | 1980 |
Guanylate cyclase activation of nitroprusside and nitrosoguanidine is related to formation of S-nitrosothiol intermediates.
Topics: Animals; Cysteine; Dithiothreitol; Enzyme Activation; Ferricyanides; Glutathione; Guanylate Cyclase; Kinetics; Liver; Mercaptoethylamines; Nitroprusside; Nitroso Compounds; Nitrosoguanidines; S-Nitrosoglutathione; S-Nitrosothiols; Structure-Activity Relationship; Thioglucosides; Valine | 1980 |
Reversible changes in the activity of inorganic pyrophosphatase of Streptococcus faecalis. The effect of compounds containing SH-groups.
Topics: Bacteriological Techniques; Cysteine; Dithiothreitol; Enterococcus faecalis; Hydrogen-Ion Concentration; Mercaptoethanol; Pyrophosphatases; Sulfhydryl Compounds; Temperature | 1981 |
Effect of physiological reducing compounds on the inactivation of tyrosine aminotransferase from guinea-pig liver.
Topics: Animals; Cysteamine; Cysteine; Dithiothreitol; Glutathione; Guinea Pigs; In Vitro Techniques; Liver; Male; Mercaptoethanol; Microsomes, Liver; NADP; Oxidation-Reduction; Tyrosine Transaminase | 1982 |
Potentiation by sulphydryl agents of the responses of guinea-pig isolated ileum to various agonists.
Topics: Acetylcholine; Animals; Cysteine; Dithiothreitol; Drug Synergism; Female; Glutathione; Glycerol; Guinea Pigs; Ileum; In Vitro Techniques; Male; Mercaptoethanol; Muscle Contraction; Nicotine; Sulfhydryl Compounds | 1984 |
Similarities in the suppression of the immune response by interferon and by a thiol-oxidizing agent.
Topics: Animals; Antibody Formation; Cysteine; Dithiothreitol; Glutathione; Immune Tolerance; Interferons; L Cells; Mercaptoethanol; Mice; Oxidation-Reduction; Spleen; Sulfhydryl Reagents | 1980 |
Reduction of copper(II)-bleomycin: a model for in vivo drug activity.
Topics: Animals; Bleomycin; Cattle; Cysteine; Dithiothreitol; DNA; Glutathione; Hydrogen-Ion Concentration; In Vitro Techniques; Models, Biological; Oxidation-Reduction | 1982 |
Differential effects of cysteine on protein and coenzyme A synthesis in rat heart.
Topics: Animals; Bicarbonates; Buffers; Carbon Radioisotopes; Coenzyme A; Cysteine; Cystine; Dextrans; Dithiothreitol; Heart; Male; Myocardium; Pantothenic Acid; Perfusion; Phenanthrolines; Phenylalanine; Protein Biosynthesis; Rats; Rats, Inbred Strains; Sulfhydryl Compounds; Sulfur Radioisotopes | 1984 |
Characterization of the cysteinyl-containing peptides of the gamma subunit of coupling factor 1.
Topics: Amino Acids; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Ethylmaleimide; Macromolecular Substances; Peptide Fragments; Plants; Proton-Translocating ATPases; Pyridines; Trypsin | 1984 |
Reaction and regulation mechanisms of selenocysteine beta-lyase.
Topics: Aluminum; Animals; Cysteine; Dithiothreitol; Keto Acids; Liver; Lyases; Magnetic Resonance Spectroscopy; Pyridoxal Phosphate; Pyridoxamine; Selenium; Selenocysteine; Swine | 1984 |
Interaction with protein SH groups could be involved in adriamycin cardiotoxicity.
Topics: Animals; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Doxorubicin; Enzyme Inhibitors; Heart; Hexokinase; Isocitrate Dehydrogenase; Malate Dehydrogenase; Male; Myocardium; Phosphofructokinase-1; Phosphogluconate Dehydrogenase; Protein Binding; Rats; Rats, Inbred Strains; Sulfhydryl Compounds | 1984 |
Regeneration of reduced-denatured seminal ribonuclease: effect of modification at cysteines 31 and 32.
Topics: Animals; Aziridines; Cattle; Cysteine; Dithiothreitol; Endonucleases; Glutathione; Iodoacetamide; Iodoacetates; Kinetics; Male; Maleimides; Oxidation-Reduction; Protein Denaturation; Ribonuclease, Pancreatic; Ribonucleases; Semen; Temperature | 1980 |
Entamoeba histolytica and Giardia lamblia: growth responses to reducing agents.
Topics: Animals; Ascorbic Acid; Cysteine; Cystine; Dithiothreitol; Entamoeba histolytica; Giardia; Oxidation-Reduction; Species Specificity; Sulfhydryl Compounds | 1981 |
Differential effects of various pharmacological agents on the cytolytic reaction mechanism of the human natural killer lymphocyte: further resolution of programming for lysis and KCIL into discrete stages.
Topics: Calcium; Cyclic AMP; Cysteine; Cytotoxicity, Immunologic; Dimethyl Sulfoxide; Dinoprostone; Dithiothreitol; Humans; Killer Cells, Natural; Mercaptoethanol; Neoplasms, Experimental; Prostaglandins E; Verapamil | 1982 |
Thiol-dependent lipid peroxidation.
Topics: Anaerobiosis; Animals; Cysteine; Cytochrome c Group; Dithiothreitol; Glutathione; Iron; Kinetics; Lipid Peroxides; Liposomes; Microsomes, Liver; Oxidation-Reduction; Rats; Superoxide Dismutase; Xanthine Oxidase | 1982 |
Evidence that copper-amino acid complexes are potent stimulators of the release of luteinizing hormone-releasing hormone from isolated hypothalamic granules.
Topics: Animals; Chelating Agents; Copper; Cysteine; Cytoplasmic Granules; Dithiothreitol; Gonadotropin-Releasing Hormone; Histidine; Hypothalamus, Middle; Kinetics; Male; Oligopeptides; Rats; Serum Albumin, Bovine; Temperature | 1984 |
Effects of oxygen and sulphydryl-containing compounds on irradiated transforming DNA. II. Glutathione, cysteine and cysteamine.
Topics: Bacillus subtilis; Cobalt Radioisotopes; Cysteamine; Cysteine; Dithiothreitol; DNA, Bacterial; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Gamma Rays; Glutathione; Oxygen; Radiation-Protective Agents; Sulfhydryl Compounds; Transformation, Bacterial | 1984 |
Reduction of DL-selenocystine and isolation of L-seleoncysteine.
Topics: Amino Acyl-tRNA Synthetases; Borohydrides; Chemistry, Organic; Cysteine; Cystine; Dithiothreitol; Mercaptoethanol; Methods; Organic Chemistry Phenomena; Organoselenium Compounds; Oxidation-Reduction; Paracoccus denitrificans; Plants; Selenium; Selenocysteine; Substrate Specificity | 1980 |
Disulfide bond formation in proteins.
Topics: Chemical Phenomena; Chemistry; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Paper; Electrophoresis, Polyacrylamide Gel; Glutathione; Indicators and Reagents; Iodoacetates; Iodoacetic Acid; Kinetics; Oxidation-Reduction; Protein Conformation; Protein Processing, Post-Translational; Proteins | 1984 |
Cysteine-dependent inactivation of hepatic ornithine decarboxylase.
Topics: Animals; Cell-Free System; Cysteine; Cytosol; Dithiothreitol; In Vitro Techniques; Iron; Liver; Male; Microsomes, Liver; Ornithine Decarboxylase Inhibitors; Rats; Rats, Inbred Strains; Spectrophotometry; Vitamin B 6 Deficiency | 1984 |
Reactions of ferryl lactoperoxidase (compound II) with sulfide and sulfhydryl compounds.
Topics: Animals; Cattle; Cysteine; Dithiothreitol; Female; Hydrogen Peroxide; Kinetics; Methimazole; Spectrophotometry; Sulfhydryl Compounds; Sulfides | 1984 |
Proteolytic cleavage of immunoglobulin by enzymes released by Fasciola hepatica.
Topics: Animals; Cysteine; Cysteine Endopeptidases; Dithiothreitol; Endopeptidases; Fasciola hepatica; Hydrogen-Ion Concentration; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Mice; Protease Inhibitors; Rabbits; Rats; Sheep; Temperature | 1982 |
Affinity labeling of a cysteine at or near the catalytic center of Escherichia coli B DNA-dependent RNA polymerase.
Topics: Affinity Labels; Binding Sites; Cysteine; Dithiothreitol; DNA-Directed RNA Polymerases; Escherichia coli; Mercaptopurine; Peptide Fragments; Pronase | 1980 |
Effects of thiol reagents on glucose transport in thymocytes.
Topics: Amino Acids; Animals; Biological Transport, Active; Calcimycin; Calcium; Cysteine; Dithiothreitol; Edetic Acid; Ethylmaleimide; Glutathione; In Vitro Techniques; Kinetics; Methylglucosides; Methylglycosides; Rats; Sulfhydryl Compounds; Thymus Gland | 1981 |
Reversal of fungitoxicity of 8-quinolinols and their copper(II) bischelates. II. Reversal of the action of 8-quinolinol by DL-alpha-lipoic acid.
Topics: Amino Acids; Aspergillus; Chelating Agents; Citric Acid Cycle; Copper; Cysteine; Dithiothreitol; Glutathione; Hydroxyquinolines; Oxyquinoline; Thioctic Acid; Vitamins | 1981 |
Attachment of [35S]cysteine to the labile site of the third component of complement via the alternative pathway.
Topics: Complement Activation; Complement C3; Complement C3b; Complement Pathway, Alternative; Cysteine; Dithiothreitol; Humans; Isotope Labeling; Sepharose | 1983 |
Alterations in pharmacological receptor activities of rabbit arteries by sulfhydryl reagents.
Topics: Animals; Calcium; Cysteine; Dithiothreitol; Ethylmaleimide; Female; In Vitro Techniques; Male; Mercaptoethanol; Mesenteric Arteries; Muscle Contraction; Muscle, Smooth, Vascular; Rabbits; Receptors, Drug; Sulfhydryl Reagents | 1983 |
Influence of reducing agents on adrenochrome-induced changes in the heart.
Topics: Adrenochrome; Animals; Ascorbic Acid; Cysteine; Dithiothreitol; Epinephrine; Heart; Male; Microscopy, Electron; Myocardium; Oxidation-Reduction; Rats; Time Factors | 1981 |
The effect of mucolytic agents on the rheologic and transport properties of canine tracheal mucus.
Topics: Acetylcysteine; Animals; Carbocysteine; Cysteine; Dithiothreitol; Dogs; Elasticity; Expectorants; In Vitro Techniques; Mucus; Potassium Iodide; Rana catesbeiana; Rheology; Trachea; Urea; Viscosity | 1980 |
C3 fragmentation in human serum: formation of a mixed disulphide between C3d and albumin.
Topics: Animals; Complement Activation; Complement C3; Complement C3d; Cysteine; Disulfides; Dithiothreitol; Humans; Immunoelectrophoresis, Two-Dimensional; Mercaptoethanol; Molecular Weight; Rabbits; Serum Albumin | 1982 |
Role of sulfhydryls in in vitro growth of Mycobacterium lepraemurium.
Topics: Adenosine Triphosphate; Chemical Phenomena; Chemistry; Culture Media; Cysteine; Dithiothreitol; Mycobacterium lepraemurium; Oxidation-Reduction; Sulfhydryl Compounds; Thioglycolates | 1981 |
The effects of cytochalasins on lymphocytes: mechanism of action of Cytochalasin A on responses to phytomitogens.
Topics: Binding Sites; Cysteine; Cytochalasin B; Cytochalasins; Dithiothreitol; Humans; Lectins; Lymphocyte Activation; Mercaptoethanol; Receptors, Mitogen | 1982 |
Increased leucoattractant binding and reversible inhibition of neutrophil motility mediated by the peroxidase/H2O2/halide system: effects of ascorbate, cysteine, dithiothreitol, levamisole and thiamine.
Topics: Adult; Ascorbic Acid; Cell Adhesion; Cell Migration Inhibition; Cysteine; Dithiothreitol; Horseradish Peroxidase; Humans; Hydrogen Peroxide; Levamisole; Neutrophils; Oxidation-Reduction; Thiamine | 1982 |
Rate-limiting step and control of coenzyme A synthesis in cardiac muscle.
Topics: Animals; Chromatography, High Pressure Liquid; Coenzyme A; Cysteine; Cytosol; Dithiothreitol; Heart; Kinetics; Male; Mitochondria, Heart; Myocardium; Pantothenic Acid; Perfusion; Rats; Rats, Inbred Strains; Subcellular Fractions | 1982 |
Contrasting effects of various sulfhydryl reagents on the activity of lecithin: cholesterol acyltransferase.
Topics: Cysteine; Dithiothreitol; Glutathione; Humans; Mercaptoethanol; Phosphatidylcholine-Sterol O-Acyltransferase; Sulfhydryl Compounds | 1981 |
Resolution of thiol-containing proteins by sequential-elution covalent chromatography.
Topics: Animals; Buffers; Cattle; Chromatography, Affinity; Chromatography, Ion Exchange; Cysteine; Disulfides; Dithiothreitol; Glutathione; Isomerases; Liver; Oxidoreductases; Protein Disulfide Reductase (Glutathione); Protein Disulfide-Isomerases | 1981 |
Feedback inhibition by methionine and S-adenosylmethionine, and desensitization of homoserine O-acetyltransferase in Brevibacterium flavum.
Topics: Acetyltransferases; Brevibacterium; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Feedback; Homoserine; Hydrogen-Ion Concentration; Hydroxymercuribenzoates; Methionine; S-Adenosylmethionine | 1981 |
Reaction of the adenine nucleotide analogue 5'-p-fluorosulfonylbenzoyl adenosine at distinct tyrosine and cysteine residues of rabbit muscle pyruvate kinase.
Topics: Adenosine; Affinity Labels; Animals; Cysteine; Dithiothreitol; Kinetics; Magnesium; Muscles; Pyruvate Kinase; Rabbits; Tyrosine | 1981 |
Effects of thiol agents on the accumulation of cadmium by rat liver parenchymal and Kupffer cells.
Topics: Animals; Cadmium; Cysteine; Dimercaprol; Dithiothreitol; Kupffer Cells; Liver; Male; Penicillamine; Rats; Sulfhydryl Reagents; Tissue Distribution | 1981 |
Elimination of thiol reagent interference during Lowry protein determination.
Topics: Colorimetry; Cysteine; Dithiothreitol; Membranes; Mercaptoethanol; Proteins; Sulfhydryl Reagents | 1981 |
Enzymatic formation of the bisfuran structure in aflatoxin biosynthesis.
Topics: Aflatoxins; Anthraquinones; Aspergillus; Chemical Phenomena; Chemistry; Cysteine; Dichlorvos; Dithiothreitol; NADP; Oxygen; Pigments, Biological | 1980 |
Capping revisited. I. Inhibition by some thiols.
Topics: Amino Acids; Animals; Concanavalin A; Cysteine; Dithiothreitol; Glycoproteins; Histocompatibility Antigens; Immunologic Capping; Isoantigens; Macromolecular Substances; Mercaptoethanol; Mice; Mice, Nude; Receptors, Antigen, B-Cell; Sulfhydryl Compounds; Thioglycolates | 1980 |
Limited proteolysis and a reactive cysteine residue define accesible regions in the native conformation of the adenovirus hexon protein.
Topics: Adenoviruses, Human; Amino Acid Sequence; Capsid Proteins; Chymotrypsin; Cyanogen Bromide; Cysteine; Dithiothreitol; Iodoacetates; Molecular Weight; Peptide Fragments; Protein Binding; Protein Conformation; Trypsin; Viral Proteins | 1980 |
Effect of titanium (III) citrate as reducing agent on growth of rumen bacteria.
Topics: Anaerobiosis; Animals; Bacteria; Bacteroides; Cysteine; Dithiothreitol; Eubacterium; Gram-Negative Anaerobic Bacteria; Oxidation-Reduction; Peptococcaceae; Rumen; Streptococcus; Titanium | 1980 |
Thiols and the diazo group in photoaffinity labels.
Topics: Affinity Labels; Cysteine; Diazonium Compounds; Dithiothreitol; Glutathione; Mercaptoethanol; Micelles; Phospholipids; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds | 1980 |
Effects of cysteine modification on the activity of the cGMP-gated channel from retinal rods.
Topics: Animals; Blotting, Western; Calcium; Calcium Channels; Cattle; Cyclic GMP; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Diamide; Dithiothreitol; Ethylmaleimide; Glutathione; Glutathione Disulfide; Ion Channel Gating; Ion Channels; Lipid Bilayers; Mersalyl; Nitric Oxide; Rod Cell Outer Segment; Sodium; Sulfhydryl Reagents | 1995 |
Mechanisms of hepatic methylmercury uptake.
Topics: Animals; Bile; Chlorides; Cysteine; Dithiothreitol; Glutathione; Ligands; Liver; Male; Methylmercury Compounds; Rats; Rats, Sprague-Dawley; Serum Albumin | 1995 |
NMDA receptor channels: subunit-specific potentiation by reducing agents.
Topics: Alanine; Animals; Base Sequence; Cell Line; Cloning, Molecular; Cysteine; Dithiothreitol; Drug Synergism; Ethylmaleimide; Glutamates; Glutamic Acid; Glutathione; Humans; Ion Channels; Kidney; Kinetics; Macromolecular Substances; Membrane Potentials; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Rats; Receptors, N-Methyl-D-Aspartate; Recombinant Fusion Proteins; Serine; Transfection | 1994 |
Identification of two cysteine residues that are required for redox modulation of the NMDA subtype of glutamate receptor.
Topics: Animals; Cysteine; Dithiothreitol; Electrophysiology; Female; Ion Channel Gating; Ion Channels; Molecular Sequence Data; Mutagenesis, Site-Directed; N-Methylaspartate; Oocytes; Oxidation-Reduction; Patch-Clamp Techniques; Rats; Receptors, N-Methyl-D-Aspartate; Recombinant Proteins; Spermine; Structure-Activity Relationship; Xenopus | 1994 |
Investigation of the nature of o-phthalaldehyde reaction with octopine dehydrogenase.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Oxidoreductases; Arginine; Binding Sites; Cysteine; Dithiothreitol; Enzyme Activation; Kinetics; Lysine; Mercaptoethanol; NAD; o-Phthalaldehyde; Protein Conformation; Pyruvates; Spectrometry, Fluorescence; Spectrophotometry; Substrate Specificity; Valine | 1994 |
Cysteine residues modify the electrophoretic mobility of amplified esterases associated with insecticide resistance in Culex mosquitoes.
Topics: Animals; Culex; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Esterases; Insecticide Resistance; Peptide Hydrolases | 1995 |
A disulfide bond between conserved extracellular cysteines in the thyrotropin-releasing hormone receptor is critical for binding.
Topics: Animals; Cells, Cultured; Cysteine; Disulfides; Dithiothreitol; Mice; Protein Conformation; Receptors, Thyrotropin-Releasing Hormone; Structure-Activity Relationship | 1995 |
Covalent modification of engineered cysteines in the nicotinic acetylcholine receptor agonist-binding domain inhibits receptor activation.
Topics: Acetylcholine; Amino Acid Sequence; Animals; Binding Sites; Bungarotoxins; Cysteine; Disulfides; Dithiothreitol; Female; Macromolecular Substances; Mice; Muscle, Skeletal; Mutagenesis, Site-Directed; Nicotinic Agonists; Nicotinic Antagonists; Oocytes; Point Mutation; Protein Engineering; Receptors, Nicotinic; Recombinant Proteins; Xenopus laevis | 1995 |
A bacterial thioredoxin-like protein that is exposed to the periplasm has redox properties comparable with those of cytoplasmic thioredoxins.
Topics: Bacterial Proteins; Base Sequence; Binding Sites; Circular Dichroism; Cloning, Molecular; Cysteine; Dithiothreitol; DNA Primers; Escherichia coli; Glutathione; Glutathione Disulfide; Kinetics; Molecular Sequence Data; Oxidation-Reduction; Protein Conformation; Recombinant Fusion Proteins; Restriction Mapping; Rhizobiaceae; Thioredoxins | 1995 |
Quantification of cysteinyl sulfhydryl residues in peptides and proteins by ESI-MS or MALDI-MS.
Topics: Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Guanidine; Guanidines; Indicators and Reagents; Mass Spectrometry; Peptides; Plant Proteins; Plants; Pyridines; Sulfhydryl Compounds | 1995 |
Oxygen free radicals enhance the nitric oxide-induced covalent NAD(+)-linkage to neuronal glyceraldehyde-3-phosphate dehydrogenase.
Topics: Animals; Cells, Cultured; Corpus Striatum; Cysteine; Dithiothreitol; Free Radicals; Glutathione; Glyceraldehyde-3-Phosphate Dehydrogenases; Mice; Molsidomine; NAD; Neurons; Nitric Oxide; Nitroprusside; Oxidation-Reduction; Oxygen; Rats; Superoxides | 1995 |
Selective disulphide linkage of plant thionins with other proteins.
Topics: Antimicrobial Cationic Peptides; Bacterial Proteins; Cysteine; Cytochrome c Group; Disulfides; Dithiothreitol; Ethylmaleimide; Glucuronidase; Glutathione; Glutathione Disulfide; Kanamycin Kinase; Ovalbumin; Peptides; Phosphotransferases (Alcohol Group Acceptor); Plant Proteins; Proteins; Pseudomonas; Serum Albumin, Bovine; Toxins, Biological | 1995 |
Intracellular transport of proinsulin in pancreatic beta-cells. Structural maturation probed by disulfide accessibility.
Topics: Animals; Cells, Cultured; Chloroquine; Cysteine; Cytoplasmic Granules; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Insulin; Islets of Langerhans; Methionine; Mice; Mice, Inbred Strains; Proinsulin; Protein Conformation; Protein Processing, Post-Translational; Sulfur Radioisotopes; Zinc | 1995 |
Disulfide bonds in a recombinant protein modeled after a core repeat in an aquatic insect's silk protein.
Topics: Amino Acid Sequence; Amino Acids; Animals; Base Sequence; Chironomidae; Cysteine; Disulfides; Dithiothreitol; Genes, Synthetic; Insect Proteins; Mass Spectrometry; Molecular Sequence Data; Peptides; Protein Denaturation; Protein Folding; Proteins; Recombinant Proteins; Repetitive Sequences, Nucleic Acid; Sequence Analysis; Silk; Sulfhydryl Compounds; Trypsin; Ultracentrifugation | 1995 |
Conformational stability of Cys45-alkylated and hydrogen peroxide-oxidised glutathione S-transferase.
Topics: Alkylation; Animals; Cysteine; Dithiothreitol; Enzyme Stability; Glutathione; Glutathione Transferase; Guanidine; Guanidines; Hydrogen Peroxide; Naphthalenesulfonates; Oxidation-Reduction; Protein Conformation; Spectrometry, Fluorescence; Structure-Activity Relationship; Swine; Thermodynamics | 1995 |
Mechanistic studies on CDP-6-deoxy-delta 3,4-glucoseen reductase: the role of cysteine residues in catalysis as probed by chemical modification and site-directed mutagenesis.
Topics: Amino Acid Sequence; Base Sequence; Binding Sites; Catalysis; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Escherichia coli; Ethylmaleimide; Gene Expression; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; NAD; Oxidoreductases; Recombinant Proteins; Structure-Activity Relationship; Trypsin; Yersinia pseudotuberculosis | 1995 |
Sulfhydryl modification of the yeast Wbp1p inhibits oligosaccharyl transferase activity.
Topics: Amino Acid Sequence; Biotin; Carbohydrate Sequence; Catalysis; Cysteine; Dithiothreitol; Enzyme Stability; Hexosyltransferases; Kinetics; Macromolecular Substances; Membrane Proteins; Mesylates; Methyl Methanesulfonate; Microsomes; Molecular Sequence Data; Polyisoprenyl Phosphate Oligosaccharides; Saccharomyces cerevisiae; Structure-Activity Relationship; Sulfhydryl Compounds; Transferases | 1995 |
Endogenous, Ca(2+)-dependent cysteine-protease cleaves specifically the ryanodine receptor/Ca2+ release channel in skeletal muscle.
Topics: Adenosine Triphosphate; Amino Acid Sequence; Animals; Calcium; Calcium Channels; Calpain; Cysteine; Dithiothreitol; Iodoacetamide; Leupeptins; Magnesium; Mercaptoethanol; Mercuric Chloride; Molecular Sequence Data; Molecular Weight; Muscle Proteins; Muscle, Skeletal; Rabbits; Ryanodine Receptor Calcium Release Channel; Sarcoplasmic Reticulum; Sodium Chloride; Substrate Specificity | 1994 |
Dithiothreitol treatment of Madin-Darby canine kidney cells reversibly blocks export from the endoplasmic reticulum but does not affect vectorial targeting of secretory proteins.
Topics: Animals; Cell Line; Cell Membrane; Clusterin; Cysteine; Dithiothreitol; Dogs; Endoplasmic Reticulum; Epithelium; Glycoproteins; Kidney; Membrane Potentials; Methionine; Molecular Chaperones; Oxidation-Reduction; Protein Conformation; Protein Folding; Protein Processing, Post-Translational; Salivary Proteins and Peptides; Sulfur Radioisotopes | 1995 |
The DNA binding activity and the dimerization ability of the thyroid transcription factor I are redox regulated.
Topics: Alkylation; Animals; Base Sequence; Binding Sites; Consensus Sequence; Cysteine; Cystine; Diamide; Dithiothreitol; DNA; DNA-Binding Proteins; Glutathione; HeLa Cells; Homeodomain Proteins; Humans; Molecular Sequence Data; Oxidation-Reduction; Promoter Regions, Genetic; Protein Conformation; Rats; Recombinant Fusion Proteins; Sequence Alignment; Sequence Homology, Nucleic Acid; Thyroid Neoplasms; Tumor Cells, Cultured | 1995 |
Extraction of extracellular L-asparaginase from Candida utilis.
Topics: Asparaginase; Candida; Culture Media; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Indicators and Reagents; Mercaptoethanol; Oxidation-Reduction; Temperature | 1995 |
Effect of metal ions on sucrose synthase from rice grains--a study on enzyme inhibition and enzyme topography.
Topics: Cations, Divalent; Chromatography, Affinity; Copper; Cysteine; Diethyl Pyrocarbonate; Dithiothreitol; Edetic Acid; Glucosyltransferases; Glutathione; Histidine; Hydrogen-Ion Concentration; Imidazoles; Kinetics; Mercaptoethanol; Oryza; Seeds | 1995 |
Modulation of transcription factor NF kappa B activity by intracellular glutathione levels and by variations of the extracellular cysteine supply.
Topics: Acetylcysteine; Animals; Base Sequence; Binding Sites; Cell Line; Cell Nucleus; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Glutathione; Glutathione Disulfide; HIV; HIV Infections; Humans; Kinetics; Macaca mulatta; Molecular Sequence Data; NF-kappa B; Oligodeoxyribonucleotides; Simian Acquired Immunodeficiency Syndrome; Simian Immunodeficiency Virus; Sulfhydryl Compounds; T-Lymphocytes; Transcriptional Activation | 1995 |
Unusual insulin binding to cells expressing an insulin receptor mutated at cysteine 524.
Topics: Amino Acids; Binding Sites; Biological Transport, Active; Cell Line; Cysteine; Dithiothreitol; Humans; In Vitro Techniques; Insulin; Kinetics; Mutagenesis, Site-Directed; Phosphorylation; Point Mutation; Protein Conformation; Receptor, Insulin | 1994 |
Modification of glutathione S-transferase 3-3 mutants with 2-(S-glutathionyl)-3,5,6-trichloro-1,4-benzoquinone. Identification of the C-terminal tryptic fragment as part of the H-site and evidence that 2-(S-glutathionyl)-3,5,6-trichloro-1,4-benzoquinone i
Topics: Amino Acid Sequence; Animals; Binding Sites; Chloranil; Cysteine; Dithiothreitol; Enzyme Inhibitors; Glutathione; Glutathione Transferase; Mass Spectrometry; Molecular Sequence Data; Mutation; Peptide Fragments; Peptide Mapping; Rats; Serine | 1994 |
Calnexin and BiP act as sequential molecular chaperones during thyroglobulin folding in the endoplasmic reticulum.
Topics: Animals; Calcium-Binding Proteins; Calnexin; Carrier Proteins; Cells, Cultured; Chaperonins; Cysteine; Dithiothreitol; Endoplasmic Reticulum; Endoplasmic Reticulum Chaperone BiP; Glycosylation; Heat-Shock Proteins; Immunoblotting; Kinetics; Macromolecular Substances; Methionine; Molecular Chaperones; Phosphoproteins; Protein Binding; Protein Folding; Sulfur Radioisotopes; Swine; Thymus Gland; Thyroglobulin | 1995 |
Triphenyltin as inductor of mitochondrial membrane permeability transition.
Topics: Animals; Calcium; Cyclosporine; Cysteine; Dithiothreitol; Intracellular Membranes; Kidney; Membrane Proteins; Mitochondria; Organotin Compounds; Permeability; Rats | 1994 |
Thioltransferase can utilize cysteamine as same as glutathione as a reductant during the restoration of cystamine-treated glucose 6-phosphate dehydrogenase activity.
Topics: Animals; Cystamine; Cysteamine; Cysteine; Dithiothreitol; Enzyme Reactivators; Glucosephosphate Dehydrogenase; Glutaredoxins; Glutathione; Humans; Liver; Mercaptoethanol; Oxidation-Reduction; Oxidoreductases; Protein Disulfide Reductase (Glutathione); Rabbits | 1994 |
Modification of cysteine residues within G(o) and other neuronal proteins by exposure to nitric oxide.
Topics: Animals; Cattle; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; GTP-Binding Proteins; In Vitro Techniques; Iodoacetamide; Mercury; Nerve Tissue Proteins; Nitric Oxide; Pertussis Toxin; Protein Binding; Rats; Synaptic Membranes; Synaptosomes; Virulence Factors, Bordetella | 1994 |
The catalytic site of monogalactosyldiacylglycerol synthase from spinach chloroplast envelope membranes. Biochemical analysis of the structure and of the metal content.
Topics: Binding Sites; Cations, Divalent; Chelating Agents; Chlorides; Chloroplasts; Cysteine; Dithiothreitol; Ethylmaleimide; Galactosyltransferases; Histidine; Intracellular Membranes; Kinetics; Molecular Weight; Spinacia oleracea; Sulfur Radioisotopes; Uridine Diphosphate; Zinc; Zinc Compounds | 1995 |
Thiol ester-linked p-coumaric acid as a new photoactive prosthetic group in a protein with rhodopsin-like photochemistry.
Topics: Chromatiaceae; Chromatography, High Pressure Liquid; Coumaric Acids; Cysteine; Disulfides; Dithiothreitol; Esters; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Photochemistry; Propionates; Rhodopsin; Sodium Hydroxide; Sulfhydryl Compounds | 1994 |
Covalent modification of the interleukin-5 receptor by isothiazolones leads to inhibition of the binding of interleukin-5.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cell Line; Cysteine; Dithiothreitol; Granulocyte-Macrophage Colony-Stimulating Factor; Interleukin-5; Kidney; Macaca; Molecular Sequence Data; Mutagenesis, Site-Directed; Receptors, Interleukin; Receptors, Interleukin-5; Recombinant Proteins; Sulfhydryl Compounds; Thiazoles | 1994 |
Thioredoxin-dependent peroxide reductase from yeast.
Topics: Amino Acid Sequence; Antioxidants; Ascorbic Acid; Base Sequence; Cloning, Molecular; Cysteine; Dithiothreitol; Fungal Proteins; Genes, Fungal; Molecular Sequence Data; Molecular Weight; Mutagenesis, Site-Directed; NADP; Neoplasm Proteins; Oxidation-Reduction; Peroxidases; Peroxiredoxins; Protein Conformation; Proteins; Saccharomyces cerevisiae; Structure-Activity Relationship; Thioredoxin-Disulfide Reductase; Thioredoxins | 1994 |
Denatured state of ovalbumin in high concentrations of urea as evaluated by disulfide rearrangement analysis.
Topics: Amino Acid Sequence; Animals; Chickens; Chromatography, High Pressure Liquid; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Female; Fluorescent Dyes; Kinetics; Molecular Sequence Data; Ovalbumin; Peptide Fragments; Peptide Mapping; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Urea | 1994 |
Mechanism-based inhibition of thymidylate synthase by 5-(trifluoromethyl)-2'-deoxyuridine 5'-monophosphate.
Topics: Amino Acid Sequence; Binding Sites; Chromatography, Gel; Chromatography, High Pressure Liquid; Collodion; Crystallization; Crystallography, X-Ray; Cysteine; Dithiothreitol; Fluorides; Lacticaseibacillus casei; Mass Spectrometry; Mercaptoethanol; Models, Molecular; Molecular Sequence Data; Molecular Structure; Spectrophotometry, Ultraviolet; Thymidylate Synthase; Thymine Nucleotides; Tyrosine | 1994 |
On the reactions of the carbapenem, meropenem, in the soluble fraction of rat kidney.
Topics: Animals; Carbapenems; Cysteine; Cytosol; Dithiothreitol; Endopeptidases; Hydrogen-Ion Concentration; Hydrolysis; Kidney; Kinetics; Magnetic Resonance Spectroscopy; Meropenem; Rats; Sulfhydryl Compounds; Thienamycins | 1994 |
Inactivation of a cytosolic phospholipase A2 by thiol-modifying reagents: cysteine residues as potential targets of phospholipase A2.
Topics: Amino Acid Sequence; Cell Line; Cysteine; Cytosol; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Stability; Humans; Hydrogen-Ion Concentration; Iodoacetamide; Kinetics; Molecular Sequence Data; Phospholipases A; Phospholipases A2; Recombinant Proteins; Sulfhydryl Reagents | 1994 |
Protective effects of sulfhydryl compounds on HOCl-induced intracellular Ca2+ increase in single rat ventricular myocytes.
Topics: Animals; Calcium; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Drug Interactions; Fura-2; Glutathione; Heart Ventricles; Hypochlorous Acid; Male; Myocardium; Rats; Rats, Sprague-Dawley; Sulfhydryl Compounds | 1994 |
Site-directed mutagenesis of human tissue transglutaminase: Cys-277 is essential for transglutaminase activity but not for GTPase activity.
Topics: Binding Sites; Cysteine; Dithiothreitol; Erythrocytes; GTP Phosphohydrolases; Humans; Iodoacetamide; Mutagenesis, Site-Directed; Recombinant Proteins; Serine; Transglutaminases | 1993 |
Mutations of noncatalytic sulfhydryl groups influence the stability, folding, and oxidative susceptibility of rhodanese.
Topics: Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cysteine; Dithiothreitol; Enzyme Stability; Escherichia coli; Kinetics; Models, Structural; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Oxidation-Reduction; Point Mutation; Protein Conformation; Protein Folding; Recombinant Proteins; Thiosulfate Sulfurtransferase; Urea | 1994 |
Replacement of the active-site cysteine residues of DsbA, a protein required for disulfide bond formation in vivo.
Topics: Base Sequence; Binding Sites; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Glutathione; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Isomerases; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Polymerase Chain Reaction; Protein Conformation; Protein Disulfide-Isomerases; Serine; Thermodynamics | 1994 |
The high affinity state of the beta 2-adrenergic receptor requires unique interaction between conserved and non-conserved extracellular loop cysteines.
Topics: Amino Acid Sequence; Animals; Binding Sites; CHO Cells; Cloning, Molecular; Conserved Sequence; Cricetinae; Cysteine; Dithiothreitol; DNA, Complementary; Electrophoresis, Polyacrylamide Gel; Immunoblotting; Iodocyanopindolol; Kinetics; Lung; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Pindolol; Point Mutation; Protein Structure, Secondary; Receptors, Adrenergic, beta-2; Transfection | 1994 |
Characterisation of haemolytic activity from Aeromonas caviae.
Topics: 2,2'-Dipyridyl; Aeromonas; Bacterial Toxins; Calcium; Cell Division; Culture Media; Cysteine; Dithiothreitol; Edetic Acid; Ferrous Compounds; Gastroenteritis; Gene Expression Regulation, Bacterial; Glutathione; Hemolysin Proteins; Humans; Magnesium; Mercaptoethanol | 1994 |
HPLC determination of glutathione and L-cysteine in pharmaceuticals after derivatization with ethacrynic acid.
Topics: Calibration; Chemistry, Pharmaceutical; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Ethacrynic Acid; Glutathione; Oxidation-Reduction; Powders; Stereoisomerism; Tablets | 1994 |
Refolding and characterization of human recombinant heparin-binding neurite-promoting factor.
Topics: Amino Acid Sequence; Animals; Carrier Proteins; Cattle; Cell Line; Cricetinae; Cysteine; Cystine; Cytokines; Dithiothreitol; DNA, Complementary; Escherichia coli; Fibroblast Growth Factors; Humans; Mesocricetus; Midkine; Molecular Sequence Data; Protein Folding; Receptors, Fibroblast Growth Factor; Recombinant Fusion Proteins; Sequence Homology, Amino Acid | 1994 |
Redox modulation of the expression of bacterial genes encoding cysteine-rich proteins in plant protoplasts.
Topics: Cells, Cultured; Cysteine; Dithiothreitol; Gene Expression Regulation, Bacterial; In Vitro Techniques; Kanamycin Kinase; Nicotiana; Oxidation-Reduction; Phosphotransferases (Alcohol Group Acceptor); Plants, Toxic; Promoter Regions, Genetic; Recombinant Proteins; RNA, Messenger; Transcription, Genetic | 1994 |
Bovine seminal plasma aSFP: localization of disulfide bridges and detection of three different isoelectric forms.
Topics: Amino Acid Sequence; Animals; Cattle; Cysteine; Disulfides; Dithiothreitol; Isoelectric Focusing; Isoelectric Point; Male; Models, Molecular; Molecular Sequence Data; Oxidation-Reduction; Peptide Fragments; Prostatic Secretory Proteins; Proteins; Semen; Seminal Plasma Proteins; Trypsin | 1994 |
Escherichia coli and Vibrio cholerae strains deficient in an enzyme involved in disulphide bond formation (DsbA) show an increase in sensitivity to dithiothreitol.
Topics: Cysteine; Dithiothreitol; Escherichia coli; Genes, Bacterial; Isomerases; Protein Disulfide-Isomerases; Vibrio cholerae | 1994 |
Effect of anti-oxidants and adsorbents on tissue browning associated metabolism in Cocculus pendulus callus cultures.
Topics: Antioxidants; Ascorbic Acid; Charcoal; Cysteine; Dithiothreitol; Plants, Medicinal; Povidone | 1993 |
Detailed study of sequence-specific DNA cleavage of triplex-forming oligonucleotides linked to 1,10-phenanthroline.
Topics: 3-Mercaptopropionic Acid; Ascorbic Acid; Base Sequence; Binding Sites; Chelating Agents; Copper; Cysteine; Dithiothreitol; DNA; Drug Stability; Glutathione; Hot Temperature; Hydrogen Bonding; Molecular Sequence Data; Molecular Structure; Nucleic Acid Conformation; Nucleic Acid Denaturation; Oligodeoxyribonucleotides; Phenanthrolines | 1994 |
Zinc-free and reduced T4 gene 32 protein binds single-stranded DNA weakly and fails to stimulate UvsX-catalyzed homologous pairing.
Topics: Apoproteins; Binding Sites; Circular Dichroism; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; DNA-Binding Proteins; DNA, Single-Stranded; Edetic Acid; Methylation; Oxidation-Reduction; Protein Binding; Protein Conformation; Recombinant Proteins; Recombination, Genetic; Structure-Activity Relationship; Viral Proteins; Zinc | 1994 |
Characterisation of a trisulphide derivative of biosynthetic human growth hormone produced in Escherichia coli.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Cloning, Molecular; Cysteine; Cystine; Dithiothreitol; Escherichia coli; Growth Hormone; Humans; Mass Spectrometry; Molecular Sequence Data; Peptide Fragments; Peptide Mapping; Recombinant Proteins; Sulfides | 1994 |
Organic hydroperoxide-induced activation of liver microsomal glutathione S-transferase of rats in vitro.
Topics: Animals; Benzene Derivatives; Cysteine; Dithiothreitol; Enzyme Activation; Glutathione Transferase; In Vitro Techniques; Lipid Peroxides; Male; Microsomes, Liver; Oxidants; Peroxides; Rats; Rats, Sprague-Dawley; Sulfhydryl Compounds; tert-Butylhydroperoxide | 1993 |
Disulfide arrangement and chemical modification of beta-1,4-endoglucanase E2 from Thermomonospora fusca.
Topics: Actinomycetales; Amino Acid Sequence; Cellobiose; Cellulase; Cellulose; Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Ethyldimethylaminopropyl Carbodiimide; Hot Temperature; Iodoacetamide; Methylation; Molecular Sequence Data; Oxidation-Reduction; Protein Denaturation | 1993 |
Disulfide arrangement and functional domains of beta-1,4-endoglucanse E5 from Thermomonospora fusca.
Topics: Actinomycetales; Amino Acid Sequence; Binding Sites; Cellulase; Cellulose; Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Iodoacetamide; Methylation; Molecular Sequence Data; Oxidation-Reduction; Peptide Fragments; Sulfhydryl Compounds | 1993 |
Reversibility of cysteine labeling by 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole.
Topics: Alkylation; Animals; Cattle; Cysteine; Dithiothreitol; Drug Stability; Fluorescent Dyes; Hydrogen-Ion Concentration; In Vitro Techniques; Kinetics; Oxadiazoles; Proteins; Serum Albumin, Bovine | 1993 |
Involvement of different cysteines in the inactivation of octopine dehydrogenase by p-chloromercuricphenyl sulfonic acid and o-phthalaldehyde.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Oxidoreductases; Binding Sites; Cysteine; Dithiothreitol; Indoles; Lysine; NAD; o-Phthalaldehyde; Spectrometry, Fluorescence | 1993 |
The secretory pathway is normal in dithiothreitol-treated cells, but disulfide-bonded proteins are reduced and reversibly retained in the endoplasmic reticulum.
Topics: alpha 1-Antitrypsin; Asialoglycoprotein Receptor; Asialoglycoproteins; Autoradiography; Calcimycin; Carcinoma, Hepatocellular; Centrifugation, Density Gradient; Cysteine; Disulfides; Dithiothreitol; Endoplasmic Reticulum; Humans; Liver Neoplasms; Macromolecular Substances; Methionine; Neoplasm Proteins; Oxidation-Reduction; Receptors, Immunologic; Ribonucleases; Serum Albumin; Subcellular Fractions; Sulfur Radioisotopes; Transferrin; Tumor Cells, Cultured | 1993 |
Thiol and disulfide determination by free zone capillary electrophoresis.
Topics: Animals; Cattle; Cystamine; Cysteamine; Cysteine; Cystine; Disulfides; Dithiothreitol; Electrophoresis; Glutathione; Glutathione Disulfide; Oxidation-Reduction; Sensitivity and Specificity; Sulfhydryl Compounds | 1993 |
Glutathione modulates the N-methyl-D-aspartate receptor-activated calcium influx into cultured rat cerebellar granule cells.
Topics: Animals; Biological Transport; Calcium; Cells, Cultured; Cerebellum; Cysteine; Dithiothreitol; Glutathione; Glutathione Disulfide; Kainic Acid; Kinetics; N-Methylaspartate; Neurons; Quisqualic Acid; Rats; Rats, Wistar; Receptors, N-Methyl-D-Aspartate | 1993 |
Triphasic vascular effects of thiol compounds and their oxidized forms on dog coronary arteries.
Topics: Animals; Coronary Vessels; Cysteamine; Cysteine; Dithiothreitol; Dogs; Endothelium, Vascular; Female; Glutathione; In Vitro Techniques; Male; Mercaptoethanol; Sulfhydryl Compounds | 1993 |
Optimization of sample recovery from the nitrocellulose support used in plasma desorption mass spectrometry and its use for multiple analyses of insulin.
Topics: Amino Acid Sequence; Animals; Cattle; Chromatography, High Pressure Liquid; Collodion; Cysteine; Dithiothreitol; Humans; Insulin; Iodine Radioisotopes; Mass Spectrometry; Membranes, Artificial; Molecular Sequence Data; Oxidation-Reduction; Polyvinyls | 1993 |
Homocysteine and other thiols in plasma and urine: automated determination and sample stability.
Topics: Anticoagulants; Autoanalysis; Chemical Precipitation; Chromatography, High Pressure Liquid; Citrates; Citric Acid; Cysteine; Dipeptides; Dithiothreitol; Drug Stability; Edetic Acid; Hemolysis; Heparin; Homocysteine; Humans; Hydrogen-Ion Concentration; Sulfhydryl Compounds | 1993 |
Overexpression of a Rhizopus delemar lipase gene in Escherichia coli.
Topics: Amino Acid Sequence; Base Sequence; Cloning, Molecular; Cysteine; Cystine; Dithiothreitol; DNA; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gene Expression; Hydrogen-Ion Concentration; Lipase; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Folding; Recombinant Proteins; Rhizopus; Solubility; Urea | 1993 |
Characterization and 2D NMR study of the stable [9-21, 15-27] 2 disulfide intermediate in the folding of the 3 disulfide trypsin inhibitor EETI II.
Topics: Amino Acid Sequence; Cysteine; Disulfides; Dithiothreitol; Hydrogen Bonding; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; Oxidation-Reduction; Plant Proteins; Protein Conformation; Protein Folding; Sequence Analysis; Trypsin Inhibitors | 1993 |
Sulfhydryl oxidation induces rapid and reversible closure of the ATP-regulated K+ channel in the pancreatic beta-cell.
Topics: Adenosine Triphosphate; Animals; Cysteine; Dithiothreitol; Ion Channel Gating; Islets of Langerhans; Mice; Mice, Obese; Oxidation-Reduction; Potassium Channels; Pyridines; Sulfhydryl Compounds; Thimerosal | 1993 |
Inactivation of ornithine decarboxylase by intermediates of tyrosinase-catalyzed reaction.
Topics: Animals; Ascorbic Acid; Catechols; Cysteine; Dithiothreitol; Female; Glutathione; Kidney; Kinetics; Melanins; Mice; Monophenol Monooxygenase; Ornithine; Ornithine Decarboxylase Inhibitors; Oxidation-Reduction; Placenta; Rats; Spectrophotometry | 1993 |
Inhibition of aspartate chemotaxis of Escherichia coli by site-directed sulfhydryl modification of the receptor.
Topics: Aspartic Acid; Bacterial Proteins; Base Sequence; Chemoreceptor Cells; Chemotaxis; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Escherichia coli; Escherichia coli Proteins; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Receptors, Cell Surface; Sulfhydryl Compounds; Threonine | 1993 |
Disulfide isoform intermediates in the reoxidation of recombinant human basic fibroblast growth factor.
Topics: Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Fibroblast Growth Factor 2; Glutathione; Humans; Isomerases; Mass Spectrometry; Molecular Structure; Oxidation-Reduction; Peptide Mapping; Protein Disulfide-Isomerases; Recombinant Proteins; Spectrometry, Mass, Fast Atom Bombardment | 1993 |
Inhibitory effects of curcumin on protein kinase C activity induced by 12-O-tetradecanoyl-phorbol-13-acetate in NIH 3T3 cells.
Topics: 3T3 Cells; Animals; Blotting, Western; Chromatography, DEAE-Cellulose; Curcumin; Cysteine; Cytosol; Dithiothreitol; Glutathione; Kinetics; Mercaptoethanol; Mice; Protein Kinase C; Subcellular Fractions; Tetradecanoylphorbol Acetate | 1993 |
Comparison of Ellman's reagent with N-(1-pyrenyl)maleimide for the determination of free sulfhydryl groups in reduced cellobiohydrolase I from Trichoderma reesei.
Topics: Cellulose 1,4-beta-Cellobiosidase; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Drug Interactions; Fluorescent Dyes; Glycoside Hydrolases; Maleimides; Mercaptoethanol; Oxidation-Reduction; Sulfhydryl Compounds; Trichoderma | 1993 |
Vibrio spp. secrete proaerolysin as a folded dimer without the need for disulphide bond formation.
Topics: Aeromonas; Aeromonas hydrophila; Bacterial Proteins; Bacterial Toxins; Base Sequence; Cysteine; Cystine; Dithiothreitol; Escherichia coli; Isomerases; Mercaptoethanol; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Pore Forming Cytotoxic Proteins; Protein Conformation; Protein Disulfide-Isomerases; Protein Folding; Protein Precursors; Recombinant Proteins; Sulfhydryl Reagents; Vibrio cholerae | 1995 |
Dynamic rearrangement of the outer mouth of a K+ channel during gating.
Topics: Cell Line; Cross-Linking Reagents; Cysteine; Dithiothreitol; Electric Conductivity; Embryo, Mammalian; Humans; Ion Channel Gating; Kidney; Macromolecular Substances; Membrane Potentials; Methyl Methanesulfonate; Mutagenesis, Site-Directed; Potassium Channels; Protein Conformation; Structure-Activity Relationship; Transfection | 1996 |
Determination and metabolism of dithiol chelating agents. XVII. In humans, sodium 2,3-dimercapto-1-propanesulfonate is bound to plasma albumin via mixed disulfide formation and is found in the urine as cyclic polymeric disulfides.
Topics: Adult; Captopril; Chelating Agents; Chromatography, Thin Layer; Cysteine; Disulfides; Dithiothreitol; Humans; Male; Penicillamine; Protein Binding; Serum Albumin; Unithiol | 1996 |
Inactivation of cholesteryl ester transfer protein by cysteine modification.
Topics: 4-Chloromercuribenzenesulfonate; Alkylating Agents; Animals; Azides; Carrier Proteins; Cell Line; Cholesterol Ester Transfer Proteins; Cholesterol Esters; Cricetinae; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Glycoproteins; Humans; Iodoacetamide; Kinetics; Pyridines; Recombinant Proteins; Structure-Activity Relationship; Sulfhydryl Reagents; Transfection | 1996 |
Glutathione is an essential metabolite required for resistance to oxidative stress in the yeast Saccharomyces cerevisiae.
Topics: Culture Media; Cysteine; Dithiothreitol; Glutathione; Mercaptoethanol; Mutation; Oxidative Stress; Saccharomyces cerevisiae | 1996 |
Studies on identifying the catalytic role of Glu-204 in the active site of yeast invertase.
Topics: Amino Acid Sequence; Bacteria; Base Sequence; beta-Fructofuranosidase; Binding Sites; Catalysis; Cysteine; Dithiothreitol; Glutamic Acid; Glycoside Hydrolases; Histidine; Hydrogen-Ion Concentration; Iodine; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Plants; Point Mutation; Recombinant Proteins; Saccharomyces cerevisiae; Sequence Homology, Amino Acid | 1996 |
Adjacent pore-lining residues within sodium channels identified by paired cysteine mutagenesis.
Topics: Amino Acid Sequence; Animals; Cadmium; Cysteine; Dithiothreitol; Electrophysiology; Female; Glutathione; Ion Channel Gating; Kinetics; Membrane Potentials; Mutagenesis, Site-Directed; Oocytes; Point Mutation; Protein Structure, Secondary; Rats; Recombinant Proteins; Sodium Channels; Xenopus laevis; Zinc | 1996 |
The oxidative inactivation of sarcoplasmic reticulum Ca(2+)-ATPase by peroxynitrite.
Topics: Animals; Borohydrides; Calcium-Transporting ATPases; Cysteine; Disulfides; Dithiothreitol; Glutathione; Kinetics; Lipid Peroxidation; Muscle Fibers, Fast-Twitch; Muscle, Skeletal; Nitrates; Oxidation-Reduction; Rabbits; Sarcoplasmic Reticulum; Trypsin | 1996 |
Dual regulation of heat-shock transcription factor (HSF) activation and DNA-binding activity by H2O2: role of thioredoxin.
Topics: Base Sequence; Cell Line; Cysteine; Dithiothreitol; DNA; DNA-Binding Proteins; Endopeptidases; Heat Shock Transcription Factors; Heat-Shock Proteins; Humans; Hydrogen Peroxide; Kinetics; Molecular Sequence Data; Nuclear Proteins; Oligodeoxyribonucleotides; Oncogene Proteins; Oncogene Proteins, Fusion; Oxidation-Reduction; Proto-Oncogene Proteins; Reactive Oxygen Species; Thioredoxins; Transcription Factors; Ubiquitin Thiolesterase | 1996 |
A disulfide bonding interaction role for cysteines in the extracellular domain of the thyrotropin-releasing hormone receptor.
Topics: Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Binding, Competitive; Cell Line; Cell Membrane; Chlorocebus aethiops; Cysteine; Disulfides; Dithiothreitol; DNA Primers; GTP-Binding Proteins; Humans; Kinetics; Molecular Sequence Data; Mutagenesis, Insertional; Mutagenesis, Site-Directed; Pituitary Gland, Anterior; Point Mutation; Polymerase Chain Reaction; Protein Structure, Secondary; Rats; Receptors, Thyrotropin-Releasing Hormone; Recombinant Proteins; Thyrotropin-Releasing Hormone; Transfection | 1996 |
S-pyridylethylation of intact polyacrylamide gels and in situ digestion of electrophoretically separated proteins: a rapid mass spectrometric method for identifying cysteine-containing peptides.
Topics: Acrylic Resins; Amino Acid Sequence; Chromatography, High Pressure Liquid; Cysteine; Databases, Factual; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Gels; Mass Spectrometry; Molecular Sequence Data; Peptides; Proteins; Pyridines; Trypsin | 1996 |
The function and properties of the iron-sulfur center in spinach ferredoxin: thioredoxin reductase: a new biological role for iron-sulfur clusters.
Topics: Chloroplasts; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electron Spin Resonance Spectroscopy; Electron Transport; Ethylmaleimide; Ferredoxins; Ferricyanides; Iron; Iron-Sulfur Proteins; NADP; Oxidoreductases; Spectrophotometry; Spectrum Analysis, Raman; Spinacia oleracea; Sulfur | 1996 |
Covalent modification of protein kinase C isozymes by the inactivating peptide substrate analog N-biotinyl-Arg-Arg-Arg-Cys-Leu-Arg-Arg-Leu. Evidence that the biotinylated peptide is an active-site affinity label.
Topics: Animals; Binding Sites; Biotin; Brain; Calcium; Cysteine; Dithiothreitol; Enzyme Inhibitors; Isoenzymes; Oligopeptides; Phosphatidylserines; Protein Binding; Protein Kinase C; Rats | 1996 |
Crystal structures of reduced, oxidized, and mutated human thioredoxins: evidence for a regulatory homodimer.
Topics: Amino Acid Sequence; Binding Sites; Crystallization; Crystallography, X-Ray; Cysteine; Disulfides; Dithiothreitol; Humans; Hydrogen Bonding; Models, Molecular; Molecular Sequence Data; Mutation; Oxidation-Reduction; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Sequence Alignment; Serine; Software; Thioredoxins | 1996 |
Identification of a potential redox-sensitive interdomain disulfide in the sedoheptulose bisphosphatase of Chlamydomonas reinhardtii.
Topics: Amino Acid Sequence; Animals; Chlamydomonas reinhardtii; Cysteine; Disulfides; Dithiothreitol; Enzyme Activation; Fructose-Bisphosphatase; Magnesium; Mercaptoethanol; Models, Molecular; Molecular Sequence Data; Oxidation-Reduction; Phosphoric Monoester Hydrolases; Phylogeny; Protein Structure, Tertiary; Reducing Agents; Sequence Alignment; Sulfhydryl Reagents; Swine | 1996 |
Human neurotrophin-3: a one-step peptide mapping method and complete disulfide characterization of the recombinant protein.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Chymotrypsin; Cysteine; Disulfides; Dithiothreitol; Escherichia coli; Guanidine; Guanidines; Humans; Molecular Sequence Data; Nerve Growth Factors; Neurotrophin 3; Peptide Fragments; Peptide Mapping; Protein Denaturation; Recombinant Proteins; Thermolysin | 1996 |
Effect of activation of protein phosphatase 1 on sulfhydryl reactivity.
Topics: Animals; Binding Sites; Cattle; Cobalt; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; In Vitro Techniques; Kinetics; Manganese; Myocardium; Phosphoprotein Phosphatases; Protein Conformation; Protein Denaturation; Protein Phosphatase 1; Recombinant Proteins; Sulfhydryl Reagents; Thiocyanates | 1996 |
Formation of protein-glutathione mixed disulfides in the developing rat conceptus following diamide treatment in vitro.
Topics: Animals; Cysteine; Diamide; Dithiothreitol; Dose-Response Relationship, Drug; Embryo, Mammalian; Embryonic and Fetal Development; Ethylmaleimide; Female; Glutathione; Organ Culture Techniques; Pregnancy; Protein Disulfide Reductase (Glutathione); Rats; Rats, Sprague-Dawley; Sulfhydryl Reagents; Tissue Distribution | 1995 |
Construction, properties and specific fluorescent labeling of a bovine prothrombin mutant engineered with a free C-terminal cysteine.
Topics: Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Carboxypeptidases; Cathepsin A; Cattle; CHO Cells; Cricetinae; Cricetulus; Cysteine; Dithiothreitol; DNA, Complementary; Enzyme Activation; Fluorescein; Fluoresceins; Fluorescent Dyes; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Peptide Hydrolases; Phosphatidylserines; Protein Conformation; Prothrombin; Recombinant Fusion Proteins; Thrombin; Thromboplastin | 1996 |
Ligand-binding characteristics and related structural features of the expressed goldfish kainate receptors: identification of a conserved disulfide bond and three residues important for ligand binding.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cells, Cultured; Cerebellum; Cloning, Molecular; Cysteine; Disulfides; Dithiothreitol; DNA Mutational Analysis; Glutamic Acid; Goldfish; Humans; Kainic Acid; Kidney; Kinetics; Ligands; Membranes; Molecular Sequence Data; Receptors, Kainic Acid; Transfection; Tritium | 1996 |
Two partially unfolded states of Torpedo californica acetylcholinesterase.
Topics: Acetylcholinesterase; Animals; Calorimetry, Differential Scanning; Chemical Phenomena; Chemistry, Physical; Cholinesterase Inhibitors; Circular Dichroism; Cysteine; Disulfides; Dithiothreitol; Electric Organ; Electron Spin Resonance Spectroscopy; Guanidine; Guanidines; Macromolecular Substances; Protein Folding; Spectrometry, Fluorescence; Sulfhydryl Reagents; Torpedo | 1996 |
Mutational analysis and chemical modification of Cys24 of lactococcin B, a bacteriocin produced by Lactococcus lactis.
Topics: Bacteriocins; Copper Sulfate; Cysteine; Dithiothreitol; Ethylmaleimide; Formates; Lactococcus lactis; Membrane Potentials; Mercuribenzoates; Mercuric Chloride; Mutagenesis, Site-Directed; Oxidants; Oxidation-Reduction; Reducing Agents | 1996 |
Species-specific inhibition of homologous enzymes by modification of nonconserved amino acids residues. The cysteine residues of triosephosphate isomerase.
Topics: Animals; Chickens; Conserved Sequence; Cysteine; Dithiothreitol; Enzyme Inhibitors; Escherichia coli; Kinetics; Models, Chemical; Models, Molecular; Molecular Sequence Data; Molecular Structure; Protein Conformation; Rabbits; Saccharomyces; Species Specificity; Sulfhydryl Reagents; Thiosulfonic Acids; Triose-Phosphate Isomerase | 1996 |
A computer program for quantification of SH groups generated after reduction of monoclonal antibodies.
Topics: Animals; Antibodies, Monoclonal; Ascorbic Acid; Carcinoembryonic Antigen; Cysteine; Dithioerythritol; Dithiothreitol; ErbB Receptors; Gastric Mucosa; Humans; Immunoglobulin G; Intestinal Mucosa; Least-Squares Analysis; Mercaptoethanol; Mice; Oxidation-Reduction; Regression Analysis; Software; Sulfhydryl Compounds; Sulfhydryl Reagents; Tin Compounds | 1996 |
Role of the cysteine residues in the alpha1,2-mannosidase involved in N-glycan biosynthesis in Saccharomyces cerevisiae. The conserved Cys340 and Cys385 residues form an essential disulfide bond.
Topics: Amino Acid Sequence; Animals; Binding Sites; Carbohydrate Sequence; Chromatography, High Pressure Liquid; Conserved Sequence; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Drosophila; Humans; Mannosidases; Mice; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Peptide Mapping; Polymerase Chain Reaction; Polysaccharides; Rabbits; Recombinant Proteins; Saccharomyces cerevisiae; Sequence Homology, Amino Acid | 1996 |
Degradation of distinct assembly forms of immunoglobulin M occurs in multiple sites in permeabilized B cells.
Topics: Animals; B-Lymphocytes; Bacterial Proteins; Brefeldin A; Cell Line; Cell Membrane Permeability; Cyclopentanes; Cysteine; Digitonin; Dithiothreitol; Endoplasmic Reticulum; Golgi Apparatus; Hybridomas; Immunoglobulin Light Chains; Immunoglobulin M; Kinetics; Methionine; Mice; Protein Synthesis Inhibitors; Streptolysins; Sulfhydryl Compounds; Sulfones | 1996 |
Identification of a cysteine residue essential for activity of protein farnesyltransferase. Cys299 is exposed only upon removal of zinc from the enzyme.
Topics: Alkyl and Aryl Transferases; Chromatography, Ion Exchange; Cysteine; Dimethylallyltranstransferase; Dithiothreitol; Ethylmaleimide; Kinetics; Mutagenesis, Site-Directed; Structure-Activity Relationship; Sulfhydryl Reagents; Transferases; Trypsin; Zinc | 1996 |
Effects of reducing agents and oxidants on excitation-contraction coupling in skeletal muscle fibres of rat and toad.
Topics: 2,2'-Dipyridyl; Animals; Bufo marinus; Calcium; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione; In Vitro Techniques; Membrane Potentials; Muscle Contraction; Muscle Fibers, Skeletal; Muscle, Skeletal; Oxidants; Rats; Reducing Agents; Sarcoplasmic Reticulum; Sulfhydryl Reagents | 1996 |
Rapid analysis of single-cysteine variants of recombinant proteins.
Topics: Animals; Cysteine; Dithiothreitol; Molecular Weight; Peptide Mapping; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 1996 |
Intracellular glutathione as a possible direct blocker of HIV type 1 reverse transcription.
Topics: Acetylcysteine; Aspartic Acid; Cysteine; Dithiothreitol; DNA, Viral; Glutathione; HIV Reverse Transcriptase; HIV-1; Humans; Transcription, Genetic; Tumor Cells, Cultured | 1996 |
Production and comparison of mature single-domain 'trefoil' peptides pNR-2/pS2 Cys58 and pNR-2/pS2 Ser58.
Topics: Amino Acid Sequence; Blotting, Western; Breast Neoplasms; Chromatography, Gel; Chromatography, Ion Exchange; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Glutathione; Humans; Molecular Sequence Data; Molecular Weight; Mutagenesis, Site-Directed; Mutation; Neoplasm Proteins; Peptides; Plasmids; Polymerase Chain Reaction; Proteins; Recombinant Proteins; Serine; Trefoil Factor-1; Tumor Suppressor Proteins | 1995 |
Analysis of the region between amino acids 30 and 42 of intact UmuD by a monocysteine approach.
Topics: Amino Acid Sequence; Bacterial Proteins; Cross-Linking Reagents; Cysteine; Dithiothreitol; DNA-Directed DNA Polymerase; Escherichia coli; Escherichia coli Proteins; Iodine; Iodoacetates; Iodoacetic Acid; Molecular Sequence Data; Mutagenesis; Phenanthrolines; Rec A Recombinases; Sequence Homology, Amino Acid; Structure-Activity Relationship; Ultraviolet Rays | 1996 |
Inhibition of RecA-mediated cleavage in covalent dimers of UmuD.
Topics: Bacterial Proteins; Cysteine; Disulfides; Dithiothreitol; DNA-Directed DNA Polymerase; Escherichia coli; Escherichia coli Proteins; Rec A Recombinases; Substrate Specificity | 1996 |
F-actin disorganization in apoptotic cell death of cultured rat renal proximal tubular cells.
Topics: Actins; Animals; Antioxidants; Apoptosis; Calcium; Cells, Cultured; Cycloheximide; Cysteine; Dactinomycin; Dithiothreitol; Kidney Tubules, Proximal; Lyases; Male; Phenylenediamines; Rats; Rats, Wistar | 1996 |
Rat liver fatty acid-binding protein: identification of a molecular species having a mixed disulfide with cysteine at cysteine-69 and enhanced protease susceptibility.
Topics: Amino Acids; Animals; Carrier Proteins; Cattle; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Endopeptidases; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Liver; Male; Myelin P2 Protein; Neoplasm Proteins; Nerve Tissue Proteins; Peptide Mapping; Rats; Rats, Sprague-Dawley | 1996 |
Sperm maturation in rhesus monkey: changes in ultrastructure, chromatin condensation, and organization of lipid bilayer.
Topics: Animals; Cell Membrane; Chromatin; Cysteine; Dithiothreitol; Ejaculation; Epididymis; Fluorescent Dyes; In Vitro Techniques; Macaca mulatta; Male; Pyrimidinones; Spermatozoa | 1997 |
Loss of GSH, oxidative stress, and decrease of intracellular pH as sequential steps in viral infection.
Topics: Adsorption; Animals; Buthionine Sulfoximine; Cell Line; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Dogs; Glutathione; Glutathione Disulfide; Hydrogen-Ion Concentration; Kidney; L-Lactate Dehydrogenase; Methionine; Oxidative Stress; Respirovirus; Sodium-Hydrogen Exchangers; Ultraviolet Rays; Virus Replication | 1997 |
Cysteine-153 is required for redox regulation of pea chloroplast fructose-1,6-bisphosphatase.
Topics: Amino Acid Sequence; Binding Sites; Catalysis; Chloroplast Thioredoxins; Chloroplasts; Cysteine; Dithiothreitol; Enzyme Activation; Fructose-Bisphosphatase; Light; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Pisum sativum; Recombinant Proteins; Thioredoxins | 1997 |
Non-enzymatic formation of formaldehyde in mouse oocyte freezing mixtures.
Topics: Animals; Cryopreservation; Cryoprotective Agents; Culture Media; Cysteine; Dimethyl Sulfoxide; Dithiothreitol; Female; Formaldehyde; Glutathione; Glycerol; Mice; Oocytes; Propylene Glycol; Propylene Glycols; Serum Albumin, Bovine; Zona Pellucida | 1996 |
Role of the Cys90, Cys95 and Cys173 residues in the structure and function of the human platelet-activating factor receptor.
Topics: Amino Acid Sequence; Animals; Azepines; Binding, Competitive; CHO Cells; COS Cells; Cricetinae; Cysteine; Dithiothreitol; DNA Primers; Humans; Inositol Phosphates; Kinetics; Models, Structural; Mutagenesis, Site-Directed; Platelet Activating Factor; Platelet Membrane Glycoproteins; Polymerase Chain Reaction; Protein Structure, Secondary; Proto-Oncogene Proteins c-myc; Receptors, Cell Surface; Receptors, G-Protein-Coupled; Recombinant Proteins; Transfection; Triazoles | 1997 |
Role of cysteine residues of rat A2a adenosine receptors in agonist binding.
Topics: Adenosine; Adenosine-5'-(N-ethylcarboxamide); Animals; Cell Membrane; Corpus Striatum; Cysteine; Dithiothreitol; Ethylmaleimide; Guanosine 5'-O-(3-Thiotriphosphate); Guanylyl Imidodiphosphate; Male; Phenethylamines; Purinergic P1 Receptor Agonists; Purinergic P1 Receptor Antagonists; Radioligand Assay; Rats; Rats, Sprague-Dawley; Receptors, Purinergic P1; Sulfhydryl Reagents | 1997 |
Thiols and neuronal nitric oxide synthase: complex formation, competitive inhibition, and enzyme stabilization.
Topics: Animals; Arginine; Biopterins; Calmodulin; Cysteine; Dithiothreitol; Enzyme Inhibitors; Enzyme Stability; Flavin-Adenine Dinucleotide; Heme; Mercaptoethanol; NADP; Neurons; Nitric Oxide Synthase; Protein Binding; Rats; Spectrometry, Fluorescence; Spectrophotometry; Sulfhydryl Compounds; Temperature | 1997 |
The hormone-responsive NADH oxidase of the plant plasma membrane has properties of a NADH:protein disulfide reductase.
Topics: 2,4-Dichlorophenoxyacetic Acid; Cell Membrane; Cysteine; Darkness; Disulfides; Dithiothreitol; Glycine max; Hypocotyl; Indoleacetic Acids; Multienzyme Complexes; NAD; NADH, NADPH Oxidoreductases; Oxidation-Reduction; Oxygen; Plant Proteins; Sulfhydryl Compounds | 1997 |
Contributing mechanisms for cysteine excitotoxicity in cultured cerebellar granule cells.
Topics: 6-Cyano-7-nitroquinoxaline-2,3-dione; Animals; Aspartic Acid; Bicarbonates; Biological Transport; Cell Death; Cells, Cultured; Cerebellum; Culture Media; Cysteine; Dithiothreitol; Dizocilpine Maleate; Glutamic Acid; Hydrogen-Ion Concentration; Kinetics; Magnesium; Neurons; Neurotoxins; Rats; Rats, Wistar | 1996 |
Cysteine reactivity in Thermoanaerobacter brockii alcohol dehydrogenase.
Topics: Alcohol Dehydrogenase; Bacteria, Anaerobic; Cysteine; Disulfides; Dithiothreitol; Electron Spin Resonance Spectroscopy; Gram-Positive Asporogenous Rods, Irregular; Guanidine; Guanidines; Iodoacetates; Iodoacetic Acid; Kinetics; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Proteins; Spin Labels; Thermodynamics | 1997 |
Identification in the mu-opioid receptor of cysteine residues responsible for inactivation of ligand binding by thiol alkylating and reducing agents.
Topics: Alkylating Agents; Alkylation; Animals; Binding Sites; Cloning, Molecular; COS Cells; Cysteine; Diprenorphine; Disulfides; Dithiothreitol; Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; Enkephalins; Ethylmaleimide; Humans; Ligands; Mutagenesis, Site-Directed; Narcotic Antagonists; Protein Binding; Receptors, Opioid, mu; Reducing Agents; Sulfhydryl Reagents; Transfection | 1997 |
An intramolecular disulfide bond between conserved extracellular cysteines in the gonadotropin-releasing hormone receptor is essential for binding and activation.
Topics: Amino Acid Sequence; Animals; COS Cells; Cysteine; Disulfides; Dithiothreitol; Enzyme-Linked Immunosorbent Assay; Kinetics; Ligands; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Rats; Receptors, LHRH; Sequence Alignment; Sulfhydryl Reagents; Surface Properties; Transfection | 1997 |
Mutational analysis of extracellular cysteine residues of rat secretin receptor shows that disulfide bridges are essential for receptor function.
Topics: Adenylyl Cyclases; Amino Acid Sequence; Animals; Cell Membrane; CHO Cells; Cricetinae; Cysteine; Disulfides; Dithiothreitol; DNA Mutational Analysis; Enzyme Activation; Molecular Sequence Data; Point Mutation; Protein Binding; Protein Sorting Signals; Protein Structure, Secondary; Rats; Receptors, G-Protein-Coupled; Receptors, Gastrointestinal Hormone; Secretin | 1997 |
Oxidized low density lipoprotein caused CNS neuron cell death.
Topics: Animals; Antidotes; Antioxidants; Biotin; Cell Death; Cells, Cultured; Cerebral Cortex; Cysteine; Deoxyuracil Nucleotides; Dithiothreitol; DNA Fragmentation; Female; Fetus; Glutathione; Hippocampus; Humans; Lipoproteins, LDL; Neuroblastoma; Oxidation-Reduction; Oxidative Stress; Rabbits; Rats; Rats, Wistar; Staining and Labeling; Sulfhydryl Reagents; Thiobarbituric Acid Reactive Substances; Tumor Cells, Cultured; Vitamin E | 1997 |
Mechanism of enhancement of slow delayed rectifier current by extracellular sulfhydryl modification.
Topics: Animals; Calcium Channels; Cells, Cultured; Cysteine; Dithiothreitol; Dogs; Female; Heart; Humans; Ion Channel Gating; Kinetics; Macromolecular Substances; Membrane Potentials; Models, Structural; Mutagenesis, Site-Directed; Myocardium; Oocytes; Point Mutation; Potassium Channels; Potassium Channels, Voltage-Gated; Protein Conformation; Protein Kinases; Recombinant Proteins; Regression Analysis; Thimerosal; Time Factors; Xenopus laevis | 1997 |
Possible thiol group involvement in intracellular pH effect on low-conductance Ca(2+)-dependent K+ channels.
Topics: Calcium; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Electric Stimulation; Ethylmaleimide; HeLa Cells; Humans; Hydrogen-Ion Concentration; Intracellular Fluid; Ion Channel Gating; Kinetics; Membrane Potentials; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Inwardly Rectifying; Probability; Sulfhydryl Reagents; Time Factors | 1997 |
A disulfide bond between conserved cysteines in the extracellular loops of the human VIP receptor is required for binding and activation.
Topics: Binding, Competitive; Cell Line; Cyclic AMP; Cysteine; Disulfides; Dithiothreitol; Humans; Mutagenesis, Site-Directed; Protein Structure, Secondary; Receptors, Vasoactive Intestinal Peptide; Structure-Activity Relationship; Transfection; Vasoactive Intestinal Peptide | 1997 |
Reduction of trans-4,5-dihydroxy-1,2-dithiane by cellular oxidoreductases activates gadd153/chop and grp78 transcription and induces cellular tolerance in kidney epithelial cells.
Topics: Animals; Carrier Proteins; CCAAT-Enhancer-Binding Proteins; Cell Line; Cell Survival; Cysteine; Disulfides; Dithiothreitol; DNA Damage; DNA-Binding Proteins; Endoplasmic Reticulum Chaperone BiP; Heat-Shock Proteins; Heterocyclic Compounds; Heterocyclic Compounds, 1-Ring; Hydrocarbons, Fluorinated; Molecular Chaperones; Nuclear Proteins; Oxidation-Reduction; Oxidoreductases; Protein Processing, Post-Translational; Radiation-Protective Agents; Stress, Physiological; Transcription Factor CHOP; Transcription Factors; Transcription, Genetic | 1997 |
A thioreduction pathway tethered to the membrane for periplasmic cytochromes c biogenesis; in vitro and in vivo studies.
Topics: Amino Acid Sequence; Bacterial Proteins; Cell Membrane; Cysteine; Cytochrome c Group; Dithiothreitol; Escherichia coli; Genes, Bacterial; Glutathione; Iodoacetamide; Iodoacetates; Iodoacetic Acid; Lyases; Membrane Proteins; Molecular Sequence Data; Oxidation-Reduction; Protein Sorting Signals; Recombinant Fusion Proteins; Rhodobacter capsulatus; Sulfhydryl Reagents | 1997 |
Cysteine residues in human lysosomal acid lipase are involved in selective cholesteryl esterase activity.
Topics: Cysteine; Dithiothreitol; Enzyme Activation; HeLa Cells; Humans; Lipase; Lysosomes; Mutagenesis, Site-Directed; Reducing Agents; Sterol Esterase; Substrate Specificity | 1997 |
Purification and characterization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase from Penicillium chrysogenum.
Topics: 2-Aminoadipic Acid; Adenosine Triphosphate; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Glucose; Hydrogen-Ion Concentration; Kinetics; Magnesium; Molecular Weight; Oligopeptides; Penicillium chrysogenum; Peptide Synthases; Protein Conformation; Valine | 1997 |
Gly166 in the NK1 receptor regulates tachykinin selectivity and receptor conformation.
Topics: Amino Acid Substitution; Animals; Binding Sites; CHO Cells; COS Cells; Cricetinae; Cysteine; Dithiothreitol; Ethylmaleimide; Glycine; Humans; Kinetics; Mutagenesis, Site-Directed; Neurokinin A; Neurokinin B; Protein Conformation; Rats; Receptors, Neurokinin-1; Receptors, Tachykinin; Recombinant Proteins; Substance P | 1997 |
The role of the buried aspartate of Escherichia coli thioredoxin in the activation of the mixed disulfide intermediate.
Topics: Amino Acid Substitution; Aspartic Acid; Bacterial Proteins; Binding Sites; Cysteine; Disulfides; Dithiothreitol; Escherichia coli; Kinetics; Oxidation-Reduction; Protein Disulfide-Isomerases; Thioredoxins | 1997 |
Increased and controlled expression of the Rickettsia prowazekii ATP/ADP translocase and analysis of cysteine-less mutant translocase.
Topics: Adenosine Triphosphate; Bacteriophage T7; Biological Transport; Cysteine; Dithiothreitol; Escherichia coli; Gene Expression; Kinetics; Mercaptoethanol; Mitochondrial ADP, ATP Translocases; Mutagenesis, Site-Directed; Mutation; Promoter Regions, Genetic; Recombinant Fusion Proteins; Reducing Agents; Rickettsia prowazekii | 1997 |
The role of the active site cysteine in catalysis by type 1 iodothyronine deiodinase.
Topics: Amino Acid Sequence; Animals; Catalysis; Conserved Sequence; Cysteine; Dithiothreitol; Glutathione; Iodide Peroxidase; Iodine; Isoenzymes; Molecular Sequence Data; Mutation; Osmolar Concentration; Propylthiouracil; Rats | 1997 |
Cancer-preventive selenocompounds induce a specific redox modification of cysteine-rich regions in Ca(2+)-dependent isoenzymes of protein kinase C.
Topics: Animals; Anticarcinogenic Agents; Binding Sites; Brain; Calcium; Cyclic AMP-Dependent Protein Kinases; Cysteine; Cystine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione; Isoenzymes; Kinetics; Nitrobenzoates; Organoselenium Compounds; Oxidation-Reduction; Phosphoprotein Phosphatases; Phosphorylase Kinase; Protein Kinase C; Protein Kinase C beta; Protein Kinase C-alpha; Protein Kinase C-delta; Protein Phosphatase 2; Rats; Sodium Selenite | 1997 |
Differential modulation of the uptake currents by redox interconversion of cysteine residues in the human neuronal glutamate transporter EAAC1.
Topics: Amino Acid Transport System X-AG; Animals; Carrier Proteins; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Evoked Potentials; Excitatory Amino Acid Transporter 3; Female; Glutamate Plasma Membrane Transport Proteins; Glutamates; Humans; Neurons; Nitrates; Oocytes; Oxidants; Oxidation-Reduction; Patch-Clamp Techniques; Symporters; Xenopus | 1997 |
Activation of the cardiac calcium release channel (ryanodine receptor) by poly-S-nitrosylation.
Topics: Animals; Calcium; Cyclic GMP; Cysteine; Dithiothreitol; Dogs; Electric Conductivity; Ethylmaleimide; Glutathione; Liposomes; Mercaptoethanol; Molsidomine; Myocardium; Nitric Oxide; Nitrosation; Nitroso Compounds; Oxidation-Reduction; Proteolipids; Ryanodine Receptor Calcium Release Channel; S-Nitrosoglutathione; S-Nitrosothiols; Sulfhydryl Compounds | 1998 |
Inhibition of AP-1 DNA binding by nitric oxide involving conserved cysteine residues in Jun and Fos.
Topics: Amino Acid Sequence; Conserved Sequence; Cysteine; Dithiothreitol; DNA; DNA Mutational Analysis; DNA-Binding Proteins; Nitric Oxide; Oxidation-Reduction; Protein Binding; Protein Processing, Post-Translational; Proto-Oncogene Proteins c-fos; Proto-Oncogene Proteins c-jun; Thioredoxins; Transcription Factor AP-1 | 1998 |
The effects of different buffers on the oxidation of DNA by thiols and ferric iron.
Topics: 8-Hydroxy-2'-Deoxyguanosine; Buffers; Cysteine; Deoxyguanosine; Dithiothreitol; DNA; Ferric Compounds; Oxidation-Reduction | 1998 |
Site-specific inactivation of aldose reductase by 4-hydroxynonenal.
Topics: Aldehyde Reductase; Aldehydes; Animals; Antioxidants; Captopril; Cattle; Cysteine; Dithiothreitol; Glutathione Disulfide; Lens, Crystalline; NADP | 1998 |
Cathepsin B, thiols and cysteine protease inhibitors in squamous-cell lung cancer.
Topics: Adult; Aged; Carcinoma, Squamous Cell; Cathepsin B; Cysteine; Cysteine Proteinase Inhibitors; Dithiothreitol; Female; gamma-Glutamyltransferase; Glutathione; Humans; Isoelectric Focusing; Lung Neoplasms; Male; Middle Aged; Sulfhydryl Compounds; Temperature; Time Factors | 1997 |
Characterization of a mammalian peroxiredoxin that contains one conserved cysteine.
Topics: Amino Acid Sequence; Conserved Sequence; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione Peroxidase; Humans; Hydrogen Peroxide; Models, Chemical; Molecular Sequence Data; Neoplasm Proteins; Oxidation-Reduction; Peroxidases; Peroxiredoxin III; Peroxiredoxins; Phospholipases A; Phospholipases A2; Proteins; Recombinant Proteins; Selenocysteine; Sequence Homology, Amino Acid; Subcellular Fractions | 1998 |
Regeneration of three-disulfide mutants of bovine pancreatic ribonuclease A missing the 65-72 disulfide bond: characterization of a minor folding pathway of ribonuclease A and kinetic roles of Cys65 and Cys72.
Topics: Animals; Cattle; Cysteine; Disulfides; Dithiothreitol; Entropy; Ethyl Methanesulfonate; Indicators and Reagents; Kinetics; Models, Chemical; Mutation; Oxidants; Oxidation-Reduction; Protein Folding; Recombinant Proteins; Ribonuclease, Pancreatic | 1998 |
Molecular orientation distributions in protein films: III. Yeast cytochrome c immobilized on pyridyl disulfide-capped phospholipid bilayers.
Topics: Adsorption; Binding Sites; Cysteine; Cytochrome c Group; Dimerization; Dithiothreitol; Lipid Bilayers; Models, Molecular; Molecular Conformation; Normal Distribution; Oxidation-Reduction; Phosphatidylcholines; Phosphatidylethanolamines; Pressure; Saccharomyces cerevisiae | 1998 |
Removal of the fluorescent 4-(aminosulfonyl)-2,1,3-benzoxadiazole label from cysteine-containing peptides.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Fluorescent Dyes; Hydrogen-Ion Concentration; Mass Spectrometry; Molecular Sequence Data; Molecular Weight; Oxadiazoles; Peptide Mapping; Peptides; Reducing Agents | 1998 |
Effects of sulfhydryl regents on the activity of lambda Ser/Thr phosphoprotein phosphatase and inhibition of the enzyme by zinc ion.
Topics: Animals; Binding Sites; Binding, Competitive; Cattle; Chelating Agents; Cysteine; Dithiothreitol; Enzyme Activation; Enzyme Inhibitors; Glycerol; Humans; Kinetics; Manganese; Mutagenesis; Nitrophenols; Organophosphorus Compounds; Oxidation-Reduction; Phosphoprotein Phosphatases; Serum Albumin, Bovine; Sulfhydryl Reagents; Tromethamine; Zinc | 1997 |
Enzyme inactivation through sulfhydryl oxidation by physiologic NO-carriers.
Topics: Amino Acid Sequence; Computer Simulation; Crystallography, X-Ray; Cysteine; Dithiothreitol; Glutathione; Glutathione Reductase; Humans; Models, Molecular; Molecular Conformation; Molecular Sequence Data; Nitric Oxide; Nitroso Compounds; Protein Conformation; S-Nitrosoglutathione; Software | 1998 |
Study of factors affecting the determination of total plasma 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD)-thiol derivatives by liquid chromatography.
Topics: Adult; Borohydrides; Chromatography, High Pressure Liquid; Cysteine; Dipeptides; Dithiothreitol; Female; Fluorescent Dyes; Fluorobenzenes; Homocysteine; Humans; Hydrogen-Ion Concentration; Male; Middle Aged; Phosphines; Reducing Agents; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence; Sulfhydryl Reagents | 1998 |
Involvement of tyrosine phosphorylation in inhibition of fMLP-induced PLD activation by N-acetyl-L-cysteine in differentiated HL60 cells.
Topics: Acetylcysteine; Androstadienes; Cell Differentiation; Chelating Agents; Cysteine; Dithiothreitol; Enzyme Activation; Free Radical Scavengers; HL-60 Cells; Humans; Inositol Phosphates; N-Formylmethionine Leucyl-Phenylalanine; Oxidants; Phosphodiesterase Inhibitors; Phospholipase D; Phosphorylation; Sulfhydryl Reagents; Tyrosine; Unithiol; Wortmannin | 1998 |
Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria.
Topics: Amino Acid Sequence; Animals; COS Cells; Cysteine; Cytosol; Disulfides; Dithiothreitol; Electron Transport Complex IV; Endoplasmic Reticulum; Fluorescent Antibody Technique; Gene Expression; Immunoglobulin Fragments; Mitochondria; Molecular Sequence Data; Oxidation-Reduction; Protein Sorting Signals; Proto-Oncogene Proteins p21(ras); ras Proteins; Recombinant Fusion Proteins; Transfection | 1995 |
delta-Aminolevulinate dehydratase inhibition by ascorbic acid is mediated by an oxidation system existing in the hepatic supernatant.
Topics: Animals; Ascorbic Acid; Binding Sites; Cysteine; Dithiothreitol; Edetic Acid; Enzyme Inhibitors; Free Radical Scavengers; Glutathione; Histidine; Liver; Mannitol; NAD; Oxidation-Reduction; Porphobilinogen Synthase; Rats; Sulfhydryl Compounds; Superoxide Dismutase | 1998 |
Formation and properties of mixed disulfides between thioredoxin reductase from Escherichia coli and thioredoxin: evidence that cysteine-138 functions to initiate dithiol-disulfide interchange and to accept the reducing equivalent from reduced flavin.
Topics: Cysteine; Disulfides; Dithionite; Dithionitrobenzoic Acid; Dithiothreitol; Escherichia coli; Flavin-Adenine Dinucleotide; Hydrogen-Ion Concentration; Mutagenesis, Site-Directed; NADP; Osmolar Concentration; Oxidation-Reduction; Protein Conformation; Protein Engineering; Spectrometry, Fluorescence; Structure-Activity Relationship; Sulfhydryl Compounds; Thioredoxin-Disulfide Reductase; Thioredoxins | 1998 |
Developmental expression and characterization of the alpha2,8-polysialyltransferase activity in embryonic chick brain.
Topics: Alkylating Agents; Amino Acid Sequence; Amino Acids, Diamino; Animals; Brain; Chick Embryo; Cholic Acids; Chromatography, Affinity; Conserved Sequence; Cysteine; Cytidine Monophosphate N-Acetylneuraminic Acid; Dithiothreitol; Gangliosides; Membrane Proteins; Oxidation-Reduction; Sialyltransferases; Solubility | 1998 |
An enzymatically active truncated form (-55 N-terminal residues) of rabbit gastric lipase. Correlation between the enzymatic activity and disulfide bond oxydo-reduction state.
Topics: Amino Acid Sequence; Animals; Cysteine; Cystine; Disulfides; Dithiothreitol; Lipase; Lipolysis; Molecular Sequence Data; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Protein Folding; Rabbits; Sequence Analysis; Stomach; Sulfhydryl Reagents; Trypsin | 1998 |
Identification and quantitation of cysteine in proteins separated by gel electrophoresis.
Topics: Alkylation; Amino Acids; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Fluorenes; Hydrolysis; Mass Spectrometry; Membranes, Artificial; Phosphines; Proteins; Sulfhydryl Reagents | 1998 |
Expression of Bcl-2 increases intracellular glutathione by inhibiting methionine-dependent GSH efflux.
Topics: Culture Media; Cysteine; Dithiothreitol; Glutamate-Cysteine Ligase; Glutathione; HeLa Cells; Humans; Kinetics; Methionine; Proto-Oncogene Proteins c-bcl-2; Recombinant Proteins; Transfection | 1998 |
The nonactive site cysteine residues of yeast protein disulfide isomerase are not required for cell viability.
Topics: Amino Acid Sequence; Animals; Binding Sites; Caffeine; Cysteine; Dithiothreitol; Mammals; Point Mutation; Protein Disulfide-Isomerases; Saccharomyces cerevisiae; Serine | 1998 |
Age-related changes of plasma glutathione and cysteine in broilers: effect of dithiothreitol reduction vitro on free and bound pools.
Topics: Aging; Animals; Blood Proteins; Chickens; Cysteine; Cystine; Dithiothreitol; Glutathione; Glutathione Disulfide; Male; Oxidation-Reduction | 1998 |
Gas chromatography/mass spectrometry analyses of [2,3,3-d3] serine, [2,3,3-d3] cysteine and [3-13 C] cysteine in plasma and skin protein: measurement of transsulphuration in young sheep.
Topics: Amino Acids, Sulfur; Animals; Blood Proteins; Cysteine; Diet; Dithiothreitol; Female; Gas Chromatography-Mass Spectrometry; Hydrolysis; Indicators and Reagents; Proteins; Serine; Sheep; Skin | 1998 |
Thioltransferase from Schizosaccharomyces pombe: purification to homogeneity and some properties.
Topics: Antidotes; Cations; Cysteine; Dithiothreitol; Enzyme Stability; Glutaredoxins; Glutathione; Hot Temperature; Hydrogen-Ion Concentration; Isoelectric Point; Kinetics; Mercaptoethanol; Metals; Molecular Weight; Oxidoreductases; Protein Disulfide Reductase (Glutathione); Schizosaccharomyces; Substrate Specificity; Sulfhydryl Reagents | 1998 |
Molecular characterization of a plant FKBP12 that does not mediate action of FK506 and rapamycin.
Topics: Amino Acid Sequence; Calcineurin; Cloning, Molecular; Cysteine; Disulfides; Dithiothreitol; Fabaceae; Genes, Plant; Genetic Complementation Test; Immunophilins; Immunosuppressive Agents; Molecular Sequence Data; Mutation; Plants, Medicinal; Protein Binding; Protein Structure, Tertiary; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sirolimus; Sulfhydryl Reagents; Tacrolimus; Tacrolimus Binding Proteins | 1998 |
Nitric oxide inhibition of the rat olfactory cyclic nucleotide-gated cation channel.
Topics: Animals; Cyclic AMP; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Ethylmaleimide; Ion Channel Gating; Ion Channels; Molsidomine; Nitric Oxide Donors; Nitroso Compounds; Olfactory Receptor Neurons; Rats; S-Nitrosothiols; Sulfhydryl Reagents | 1998 |
Regulation of cyclic GMP and cyclic AMP production by S-nitroso-cysteine in rat thymocytes.
Topics: Adenylyl Cyclase Inhibitors; Animals; Cell Membrane; Cyclic AMP; Cyclic GMP; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Male; Nitric Oxide; Nitroso Compounds; Rats; Rats, Sprague-Dawley; S-Nitrosothiols; Sulfhydryl Reagents; Thymus Gland | 1998 |
Glyceraldehyde-3-phosphate dehydrogenase inactivation by peroxynitrite.
Topics: Animals; Arsenites; Binding, Competitive; Cysteine; Dithiothreitol; Enzyme Inhibitors; Free Radical Scavengers; Glutathione; Glyceraldehyde 3-Phosphate; Glyceraldehyde-3-Phosphate Dehydrogenases; Hydrogen-Ion Concentration; Iodobenzoates; Kinetics; Methionine; Muscle, Skeletal; Nitrates; Nitric Oxide; Oxidation-Reduction; Rabbits; Sulfhydryl Compounds | 1998 |
pH-dependent gating of ROMK (Kir1.1) channels involves conformational changes in both N and C termini.
Topics: Amino Acid Substitution; Animals; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Female; Hydrogen-Ion Concentration; In Vitro Techniques; Ion Channel Gating; Kidney; Membrane Potentials; Mutagenesis, Site-Directed; Oocytes; Oxidation-Reduction; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Inwardly Rectifying; Protein Conformation; Recombinant Proteins; Xenopus laevis | 1998 |
Thioltransferase from Arabidopsis thaliana seed: purification to homogeneity and characterization.
Topics: Arabidopsis; Cysteine; Dithiothreitol; Enzyme Activation; Enzyme Stability; Glutaredoxins; Glutathione; Hot Temperature; Hydrogen-Ion Concentration; Isoelectric Point; Mercaptoethanol; Molecular Weight; Oxidoreductases; Protein Disulfide Reductase (Glutathione); Seeds; Substrate Specificity; Sulfhydryl Reagents | 1998 |
Thiol-mediated disassembly and reassembly of [2Fe-2S] clusters in the redox-regulated transcription factor SoxR.
Topics: Apoproteins; Bacterial Proteins; Cysteine; Dithiothreitol; Electron Spin Resonance Spectroscopy; Escherichia coli; Glutathione; Iron-Sulfur Proteins; Oxidation-Reduction; Sulfhydryl Compounds; Thioredoxins; Transcription Factors | 1998 |
Total plasma homocysteine as part of the routine aminogram by ion-exchange chromatography.
Topics: Amino Acids; Blood Chemical Analysis; Chromatography, Ion Exchange; Cysteine; Dithiothreitol; Glutamic Acid; Glutamine; Homocysteine; Humans; Time Factors | 1998 |
Palmitoylation of the rat mu opioid receptor.
Topics: Amino Acid Sequence; Animals; Binding Sites; CHO Cells; Cricetinae; Cysteine; Dithiothreitol; Hydroxylamine; Kinetics; Molecular Sequence Data; Morphine; Mutagenesis, Site-Directed; Palmitic Acid; Peptide Fragments; Protein Processing, Post-Translational; Rats; Receptors, Opioid, mu; Recombinant Proteins; Transfection | 1998 |
Conserved cysteines of the human immunodeficiency virus type 1 protease are involved in regulation of polyprotein processing and viral maturation of immature virions.
Topics: Alanine; Amino Acid Sequence; Amino Acid Substitution; Animals; Conserved Sequence; COS Cells; Cysteine; Diamines; Dithiothreitol; Gene Products, gag; HIV Protease; HIV Protease Inhibitors; HIV-1; Humans; Microtomy; Molecular Sequence Data; Oligopeptides; Protein Precursors; Protein Processing, Post-Translational; Proteins; Virion; Virus Assembly | 1999 |
Sample preparation for peptide mapping--A pharmaceutical quality-control perspective.
Topics: Alkylation; Aziridines; Chemistry, Pharmaceutical; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Indicators and Reagents; Interferons; Iodoacetamide; Iodoacetic Acid; Oxidation-Reduction; Peptide Mapping; Peptides; Phosphines; Protein Denaturation; Pyridines; Quality Control; Solubility; Solvents; Trypsin | 1999 |
Functional analysis of conserved cysteines in heparan sulfate N-deacetylase-N-sulfotransferases.
Topics: Alanine; Amidohydrolases; Amino Acid Substitution; Animals; Catalysis; Cysteine; Dithiothreitol; Enzyme Inhibitors; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Liver; Rats; Recombinant Proteins; Sulfotransferases | 1999 |
Carbamylation of cysteine: a potential artifact in peptide mapping of hemoglobins in the presence of urea.
Topics: Alkylation; Amino Acid Sequence; Cysteine; Dithiothreitol; Gas Chromatography-Mass Spectrometry; Hemoglobins; Humans; Hydrogen-Ion Concentration; Hydroxylamine; Peptide Mapping; Recombinant Proteins; Serine Endopeptidases; Urea | 1999 |
An unconventional role for cytoplasmic disulfide bonds in vaccinia virus proteins.
Topics: Antiviral Agents; Cysteine; Cytoplasm; Cytosol; Dimerization; Disulfides; Dithiothreitol; Ethylmaleimide; HeLa Cells; Humans; Kinetics; Sulfhydryl Reagents; Tritium; Vaccinia virus; Viral Core Proteins; Viral Envelope Proteins | 1999 |
Respiratory chain strongly oxidizes the CXXC motif of DsbB in the Escherichia coli disulfide bond formation pathway.
Topics: Bacterial Proteins; Binding Sites; Cysteine; Disulfides; Dithiothreitol; Electron Transport; Escherichia coli; Membrane Proteins; Mutation; Oxidation-Reduction; Oxygen; Protein Disulfide-Isomerases; Stilbenes; Sulfonic Acids | 1999 |
Modifications of Ca2+ mobilization and noradrenaline release by S-nitroso-cysteine in PC12 cells.
Topics: Adenosine Triphosphate; Animals; Biological Transport; Calcium; Cysteine; Dithiothreitol; Ethylmaleimide; Nitric Oxide Donors; Nitroso Compounds; Norepinephrine; PC12 Cells; Rats; Receptors, Purinergic; S-Nitrosothiols; Sulfhydryl Compounds | 1999 |
Effects of mercury on the isolated heart muscle are prevented by DTT and cysteine.
Topics: Animals; Cysteine; Dithiothreitol; Heart; In Vitro Techniques; Isometric Contraction; Mercury; Myocardial Contraction; Myocardium; Myosins; Papillary Muscles; Rats; Sulfhydryl Reagents | 1999 |
Heat stability of the potato tuber ADP-glucose pyrophosphorylase: role of Cys residue 12 in the small subunit.
Topics: Adenosine Diphosphate Glucose; Amino Acid Sequence; Amino Acid Substitution; Cysteine; Disulfides; Dithiothreitol; Enzyme Stability; Glucose-1-Phosphate Adenylyltransferase; Hot Temperature; Molecular Sequence Data; Nucleotidyltransferases; Oxidation-Reduction; Plant Roots; Protein Binding; Protein Conformation; Sequence Alignment; Sequence Deletion; Solanum tuberosum | 1999 |
A two-component tetrachlorohydroquinone reductive dehalogenase system from the lignin-degrading basidiomycete Phanerochaete chrysosporium.
Topics: Cell Fractionation; Cell Membrane; Chlorine; Chlorophenols; Chromatography, High Pressure Liquid; Cysteine; Cytosol; Dithiothreitol; Glutathione; Glutathione Transferase; Halogens; Hydrogen-Ion Concentration; Hydroquinones; Lignin; Mass Spectrometry; Oxidoreductases; Phanerochaete; Reducing Agents; Solubility; Substrate Specificity; Temperature | 1999 |
Light-dependent changes in redox status of the plastidic acetyl-CoA carboxylase and its regulatory component.
Topics: Acetyl-CoA Carboxylase; Carbon-Nitrogen Ligases; Carboxyl and Carbamoyl Transferases; Carrier Proteins; Cell Extracts; Chloroplasts; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Fatty Acid Synthase, Type II; Hydrogen-Ion Concentration; Light; Malonyl Coenzyme A; Multienzyme Complexes; Oxidation-Reduction; Pisum sativum; Plant Leaves; Time Factors | 1999 |
Oxidative refolding of recombinant prochymosin.
Topics: Air; Chymosin; Circular Dichroism; Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Enzyme Precursors; Escherichia coli; Glutathione; Hydrogen-Ion Concentration; Inclusion Bodies; Models, Chemical; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Protein Denaturation; Protein Folding; Recombinant Proteins; Solubility; Spectrometry, Fluorescence; Sulfhydryl Compounds; Urea | 1999 |
Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking.
Topics: Alleles; Amino Acid Substitution; Arabinose; Bacterial Proteins; Binding Sites; Cell Membrane Permeability; Cysteine; Disulfides; Dithiothreitol; Escherichia coli; Escherichia coli Proteins; Genes, Dominant; Genes, Lethal; Membrane Proteins; Molecular Weight; Mutagenesis; Periplasm; Phenotype; Protein Binding; Protein Conformation; Protein Disulfide-Isomerases; SEC Translocation Channels | 1999 |
N-terminal domains of fibrillin 1 and fibrillin 2 direct the formation of homodimers: a possible first step in microfibril assembly.
Topics: Alkylation; Animals; Blotting, Western; CHO Cells; Cricetinae; Cysteine; Dimerization; Disulfides; Dithiothreitol; Fibrillins; Glycine; Microfilament Proteins; Molecular Weight; Peptide Fragments; Precipitin Tests; Proline; Protein Binding; Sequence Deletion; Time Factors; Transfection | 1999 |
Chloroplast thioredoxin mutants without active-site cysteines facilitate the reduction of the regulatory disulphide bridge on the gamma-subunit of chloroplast ATP synthase.
Topics: Binding Sites; Chloroplast Thioredoxins; Chloroplasts; Cysteine; Disulfides; Dithiothreitol; Enzyme Activation; Models, Biological; Mutation; Oxidation-Reduction; Protein Binding; Proton-Translocating ATPases; Spinacia oleracea; Sulfhydryl Compounds; Thioredoxins | 1999 |
Critical role of sulfenic acid formation of thiols in the inactivation of glyceraldehyde-3-phosphate dehydrogenase by nitric oxide.
Topics: Binding Sites; Cysteine; Dithiothreitol; Enzyme Repression; Glyceraldehyde-3-Phosphate Dehydrogenases; Hydrogen Peroxide; NAD; Nitric Oxide; Nitric Oxide Donors; Nitroprusside; Oxidation-Reduction; Penicillamine; Sulfenic Acids; Sulfhydryl Compounds | 1999 |
Reactivity of biologically important thiol compounds with superoxide and hydrogen peroxide.
Topics: Acetylcysteine; Angiotensin-Converting Enzyme Inhibitors; Captopril; Cysteamine; Cysteine; Dithiothreitol; Glutathione; Hydrogen Peroxide; Oxygen Consumption; Penicillamine; Reactive Oxygen Species; Sulfhydryl Compounds; Superoxides; Xanthine Oxidase | 1999 |
Zinc finger of replication protein A, a non-DNA binding element, regulates its DNA binding activity through redox.
Topics: Amino Acid Sequence; Chelating Agents; Cysteine; Diamide; Dithiothreitol; DNA Helicases; DNA-Binding Proteins; DNA, Single-Stranded; Humans; Models, Molecular; Molecular Sequence Data; Mutation; Oxidation-Reduction; Phenanthrolines; Proteins; Sulfhydryl Reagents; Thioredoxins; Trans-Activators; Zinc Fingers | 1999 |
Tandem heterocyclization activity of the multidomain 230 kDa HMWP2 subunit of Yersinia pestis yersiniabactin synthetase: interaction of the 1-1382 and 1383-2035 fragments.
Topics: Acyl Carrier Protein; Amino Acid Sequence; Bacterial Outer Membrane Proteins; Bacterial Proteins; Carrier Proteins; Catalysis; Cloning, Molecular; Cysteine; Dithiothreitol; Iron-Binding Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Periplasmic Binding Proteins; Phenols; Protein Structure, Tertiary; Siderophores; Substrate Specificity; Sulfhydryl Compounds; Thiazoles; Yersinia pestis | 1999 |
Redox regulation of calcineurin in T-lymphocytes.
Topics: Actins; Calcineurin; Cysteine; Dithiothreitol; Electron Spin Resonance Spectroscopy; Gene Expression Regulation; Genes, Reporter; Humans; Hydrogen Peroxide; Jurkat Cells; Mutation; Oxidation-Reduction; Phosphoprotein Phosphatases; Promoter Regions, Genetic; T-Lymphocytes; Temperature; Time Factors; Transcription Factor AP-1; Transcription, Genetic | 1999 |
Effect of glutathione L-cystein and L-djenkolic acid in the synthesis and mutagenicity of azide metabolite in Bacillus subtilis ATCC 6633 strain.
Topics: Bacillus subtilis; Cysteine; Dithiothreitol; Glutathione; Mutagens; Serine; Sodium Azide | 1999 |
Prion protein glycosylation is sensitive to redox change.
Topics: Cysteine; Disulfides; Dithiothreitol; Endoplasmic Reticulum; Glycosylation; Humans; Immunoblotting; Mutagenesis; Oxidation-Reduction; Oxidative Stress; Precipitin Tests; Prions; PrPC Proteins; Time Factors; Transfection; Tumor Cells, Cultured | 1999 |
Efflux of T4 from the in situ perfused liver of rainbow trout: effect of T4, dithiothreitol and cysteine in the perfusate.
Topics: Animals; Cysteine; Dithiothreitol; Kinetics; Liver; Oncorhynchus mykiss; Perfusion; Thyroxine | 1999 |
Oxidation and reduction of pig skeletal muscle ryanodine receptors.
Topics: Animals; Biophysical Phenomena; Biophysics; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; In Vitro Techniques; Malignant Hyperthermia; Muscle, Skeletal; Myocardium; Oxidation-Reduction; Point Mutation; Pyridines; Ryanodine Receptor Calcium Release Channel; Sulfhydryl Reagents; Swine; Swine Diseases | 1999 |
Assignment of a single disulfide bridge in rat liver methionine adenosyltransferase.
Topics: Amino Acid Sequence; Animals; Cellulase; Cysteine; Dimerization; Disulfides; Dithiothreitol; Ethylmaleimide; Liver; Methionine Adenosyltransferase; Molecular Sequence Data; Nitric Oxide Donors; Peptide Fragments; Peptide Mapping; Protein Denaturation; Protein Structure, Quaternary; Rats; Sequence Analysis, Protein; Trypsin; Urea | 2000 |
Functional analysis of a disulfide bond in the cardiac Na(+)-Ca(2+) exchanger.
Topics: Cysteine; Disulfides; Dithiothreitol; Electric Conductivity; Electrophoresis, Polyacrylamide Gel; Ferrous Compounds; Mutagenesis, Site-Directed; Myocardium; Oxidation-Reduction; Patch-Clamp Techniques; Recombinant Proteins; Sodium-Calcium Exchanger | 2000 |
Cysteine 111 affects coupling of single-stranded DNA binding to ATP hydrolysis in the herpes simplex virus type-1 origin-binding protein.
Topics: Adenosine Triphosphate; Alanine; Amino Acid Sequence; Cysteine; Dithiothreitol; DNA Helicases; DNA Replication; DNA-Binding Proteins; DNA, Single-Stranded; Ethylmaleimide; Hydrolysis; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Sequence Homology, Amino Acid; Viral Proteins | 2000 |
Cysteine-scanning mutagenesis of the periplasmic loop regions of PomA, a putative channel component of the sodium-driven flagellar motor in Vibrio alginolyticus.
Topics: Amino Acid Sequence; Amino Acid Substitution; Bacterial Outer Membrane Proteins; Biotin; Cysteine; Dithiothreitol; Electroporation; Flagella; Maleimides; Molecular Sequence Data; Mutagenesis; Protein Structure, Secondary; Sodium; Vibrio | 2000 |
Reactivity of the cysteine residues in the protein splicing active center of the Mycobacterium tuberculosis RecA intein.
Topics: Amino Acid Substitution; Bacterial Proteins; Binding Sites; Cysteine; Dithiothreitol; Fungal Proteins; Hydrogen-Ion Concentration; Hydroxylamine; Iodoacetamide; Mycobacterium tuberculosis; Protein Splicing; Rec A Recombinases; Recombinant Fusion Proteins; Saccharomyces cerevisiae; Structure-Activity Relationship; Sulfhydryl Compounds; Sulfhydryl Reagents | 2000 |
[Regeneration of functionally active rat brain muscarinic receptor in vitro after inhibition with methylmercury chloride].
Topics: Animals; Binding Sites; Brain; Cysteine; Dithiothreitol; Glutathione; In Vitro Techniques; Male; Methylmercury Compounds; Quinuclidinyl Benzilate; Rats; Rats, Sprague-Dawley; Receptors, Muscarinic; Sulfhydryl Reagents; Unithiol | 1997 |
Importance of cysteine residues for the stability and catalytic activity of human pancreatic beta cell glucokinase.
Topics: Alloxan; Base Sequence; Binding Sites; Catalysis; Cysteine; Disulfides; Dithiothreitol; Endopeptidase K; Enzyme Stability; Glucokinase; Hexoses; Humans; Inhibitory Concentration 50; Islets of Langerhans; Kinetics; Molecular Weight; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Proteins; Sulfhydryl Compounds; Sulfhydryl Reagents; Temperature | 2000 |
Laccase activity tests and laccase inhibitors.
Topics: Benzothiazoles; Cysteine; Dithiothreitol; Ditiocarb; Enzyme Inhibitors; Laccase; Oxidation-Reduction; Oxidoreductases; Oxygen Consumption; Pyrogallol; Sodium Azide; Sulfonic Acids; Thioglycolates | 2000 |
Structure-function relationships in the carboxylic-ester-hydrolase superfamily. Disulfide bridge arrangement in porcine intestinal glycerol-ester hydrolase.
Topics: Animals; Binding Sites; Carboxylic Ester Hydrolases; Cholinesterases; Cyanogen Bromide; Cysteine; Disulfides; Dithiothreitol; Enzyme Stability; Intestinal Mucosa; Iodoacetamide; Lipase; Peptide Fragments; Peptide Mapping; Protein Binding; Protein Conformation; Sequence Analysis; Structure-Activity Relationship; Substrate Specificity; Swine | 2000 |
Two critical cysteine residues implicated in disulfide bond formation and proper folding of Kir2.1.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Conserved Sequence; Cysteine; Disulfides; Dithiothreitol; Electric Conductivity; Genes, Dominant; Models, Molecular; Molecular Sequence Data; Molecular Weight; Mutation; Oocytes; Oxidation-Reduction; Potassium Channels; Potassium Channels, Inwardly Rectifying; Protein Folding; Protein Structure, Quaternary; Protein Structure, Secondary; Reducing Agents; Sequence Alignment; Serine Endopeptidases; Structure-Activity Relationship; Xenopus laevis | 2000 |
Inactivation of creatine kinase by S-glutathionylation of the active-site cysteine residue.
Topics: Amino Acid Sequence; Animals; Binding Sites; Chromatography, High Pressure Liquid; Creatine Kinase; Cysteine; Dithiothreitol; Glutathione; Glutathione Disulfide; Isoenzymes; Kinetics; Molecular Sequence Data; Molecular Weight; Muscle, Skeletal; Oxidative Stress; Peptide Fragments; Rabbits; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds; Thermodynamics; Trypsin | 2000 |
Inhibition of papain by S-nitrosothiols. Formation of mixed disulfides.
Topics: Binding Sites; Cysteine; Disulfides; Dithiothreitol; Enzyme Activation; Enzyme Inhibitors; Fluorescent Dyes; Glutathione; Kinetics; Mercaptoethanol; Molecular Structure; Nitric Oxide; Nitroso Compounds; Papain; S-Nitrosoglutathione; S-Nitrosothiols; Spectrometry, Fluorescence; Sulfhydryl Compounds | 2000 |
Drug-stimulated ATPase activity of human P-glycoprotein is blocked by disulfide cross-linking between the nucleotide-binding sites.
Topics: Adenosine Triphosphatases; ATP Binding Cassette Transporter, Subfamily B, Member 1; Binding Sites; Cell Line; Cell Membrane; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Humans; Models, Biological; Mutagenesis; Nucleotides; Oxygen; Protein Structure, Tertiary; Sulfhydryl Reagents; Verapamil | 2000 |
Probing ligand-induced conformational changes of human CD38.
Topics: ADP-ribosyl Cyclase; ADP-ribosyl Cyclase 1; Antigens, CD; Antigens, Differentiation; Binding Sites; Catalysis; Cysteine; Disulfides; Dithiothreitol; Epitopes; Flow Cytometry; HL-60 Cells; Humans; Kinetics; Ligands; Membrane Glycoproteins; Models, Biological; NAD; NAD+ Nucleosidase; Protein Binding; Protein Conformation; Transfection; Trypsin | 2000 |
Cystine knot mutations affect the folding of the glycoprotein hormone alpha-subunit. Differential secretion and assembly of partially folded intermediates.
Topics: Alanine; Amino Acid Substitution; Cell Line; Cysteine; Cystine; Disulfides; Dithiothreitol; Glycoprotein Hormones, alpha Subunit; Humans; Models, Molecular; Mutagenesis, Site-Directed; Point Mutation; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Transfection | 2000 |
Effects of low-level lead on glycolytic enzymes and pyruvate dehydrogenase of rat brain in vitro: relevance to sporadic Alzheimer's disease?
Topics: Adenosine Triphosphate; Alzheimer Disease; Animals; Brain; Chelating Agents; Cysteine; Dithiothreitol; Edetic Acid; Enzyme Activation; Glyceraldehyde-3-Phosphate Dehydrogenases; Glycolysis; Hexokinase; In Vitro Techniques; Lead; Male; Organometallic Compounds; Pyruvate Dehydrogenase Complex; Pyruvate Kinase; Rats; Rats, Wistar | 2000 |
Inactivation of rabbit muscle creatine kinase by reversible formation of an internal disulfide bond induced by the fungal toxin gliotoxin.
Topics: Animals; Creatine Kinase; Cysteine; Dimerization; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Fungal Proteins; Gliotoxin; Glutathione; Immunosuppressive Agents; Models, Biological; Muscles; Oxidation-Reduction; Oxygen; Rabbits; Reducing Agents; Sulfhydryl Compounds; Time Factors | 2000 |
Nitric oxide and thiol reagent modulation of Ca2+-activated K+ (BKCa) channels in myocytes of the guinea-pig taenia caeci.
Topics: Animals; Calcium; Cecum; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Glutathione; Guinea Pigs; Immunohistochemistry; In Vitro Techniques; Membrane Potentials; Muscle, Smooth; Nitric Oxide; Nitric Oxide Donors; Nitroprusside; Nitroso Compounds; Oxidation-Reduction; Potassium Channels; Pyridines; S-Nitrosothiols; Sulfhydryl Reagents; Thimerosal | 2000 |
Contamination of an anion-exchange membrane by glutathione.
Topics: Adsorption; Anions; Cysteine; Dialysis; Dithiothreitol; Glutathione; Hydrogen-Ion Concentration; Membranes, Artificial; Oxidation-Reduction | 2000 |
Redox modulation of recombinant human GABA(A) receptors.
Topics: Animals; Chelating Agents; Cysteine; Dithiothreitol; Humans; Oocytes; Oxidation-Reduction; Receptors, GABA-A; Recombinant Proteins; Xenopus laevis; Zinc | 2000 |
Effects of dithiothreitol on protein activity unrelated to thiol-disulfide exchange: for consideration in the analysis of protein function with Cleland's reagent.
Topics: Amino Acid Sequence; Animals; Base Sequence; Carbohydrate Metabolism; Cattle; Cloning, Molecular; Cysteine; Disulfides; Dithiothreitol; DNA-Binding Proteins; Edetic Acid; Enzyme Inhibitors; Extracellular Matrix Proteins; Glycoproteins; Lectins; Molecular Sequence Data; Nuclear Proteins; Phenanthrolines; Plasmids; Protein Conformation; Protein Structure, Tertiary; Recombinant Proteins; RNA-Binding Proteins; Sea Urchins; Sugar Alcohols; Sulfhydryl Compounds | 2000 |
Cysteine 230 modulates tumor necrosis factor-related apoptosis-inducing ligand activity.
Topics: Apoptosis; Apoptosis Regulatory Proteins; Blotting, Western; Cysteine; Dimerization; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Glycine; HeLa Cells; Humans; Membrane Glycoproteins; Mutagenesis, Site-Directed; Protein Binding; Recombinant Proteins; TNF-Related Apoptosis-Inducing Ligand; Tumor Necrosis Factor-alpha | 2000 |
Role of glutathione in the formation of the active form of the oxygen sensor FNR ([4Fe-4S].FNR) and in the control of FNR function.
Topics: Bacterial Proteins; Cysteine; Dithiothreitol; Escherichia coli; Escherichia coli Proteins; Genes, Reporter; Genotype; Glutaredoxins; Glutathione; Glutathione Reductase; Iron; Iron-Sulfur Proteins; Kinetics; Models, Biological; Oxidoreductases; Oxygen; Plasmids; Proteins; Recombinant Fusion Proteins; Spectrophotometry; Time Factors; Ultraviolet Rays | 2000 |
Expression and mutagenesis of the novel serpin endopin 2 demonstrates a requirement for cysteine-374 for dithiothreitol-sensitive inhibition of elastase.
Topics: Amino Acid Sequence; Animals; Cattle; Cysteine; Dithiothreitol; Drug Synergism; Glutathione; Humans; Maleimides; Molecular Sequence Data; Mutagenesis, Site-Directed; Pancreatic Elastase; Protease Inhibitors; Protein Conformation; Recombinant Proteins; Reducing Agents; Sequence Homology, Amino Acid; Serpins; Structure-Activity Relationship | 2000 |
Inhibition of glutamate uptake by Tx3-4 is dependent on the redox state of cysteine residues.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Biological Transport; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Glutamic Acid; Hippocampus; Neuropeptides; Neurotoxins; Oxidation-Reduction; Rats; Rats, Wistar; Spider Venoms; Synaptosomes | 2000 |
Activation of thiol-dependent antioxidant activity of human serum albumin by alkaline pH is due to the B-like conformational change.
Topics: Antioxidants; Cysteine; Dithiothreitol; Humans; Hydrogen-Ion Concentration; In Vitro Techniques; Lipid Peroxides; Peroxidases; Protein Conformation; Serum Albumin; Sulfhydryl Compounds; Sulfhydryl Reagents | 2000 |
Polymorphism of the glutathione transferase subunit 3 in Sprague-Dawley rats involves a reactive cysteine residue.
Topics: Animals; Chromatography, Agarose; Chromatography, High Pressure Liquid; Cloning, Molecular; Cysteine; Dithiothreitol; DNA, Complementary; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Glutathione; Glutathione Transferase; Hydrogen Peroxide; Hydrogen-Ion Concentration; Immunoblotting; Liver; Male; Oxidative Stress; Polymorphism, Genetic; Rats; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, Protein; Species Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Substrate Specificity; Time Factors; Trypsin; Xanthine; Xanthine Oxidase | 2000 |
A sulfenic acid enzyme intermediate is involved in the catalytic mechanism of peptide methionine sulfoxide reductase from Escherichia coli.
Topics: Binding Sites; Catalysis; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Escherichia coli; Methionine; Methionine Sulfoxide Reductases; Models, Chemical; Molecular Weight; Mutation; Oxidoreductases; Peptides; Reducing Agents; Spectrometry, Mass, Electrospray Ionization; Sulfenic Acids; Sulfhydryl Compounds; Thioredoxins | 2000 |
Block of sodium channels by divalent mercury: role of specific cysteinyl residues in the P-loop region.
Topics: 2,2'-Dipyridyl; Amino Acid Substitution; Animals; Cysteine; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Humans; Membrane Potentials; Mercuric Chloride; Muscle, Skeletal; Mutagenesis, Site-Directed; Patch-Clamp Techniques; Rats; Recombinant Proteins; Sodium Channels; Thimerosal; Transfection | 2000 |
Suitable experimental conditions are required to characterize interferon-alpha2b synthetic peptides.
Topics: Binding Sites; Cell Division; Cell Line; Cell Membrane; Chromatography, Gel; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Epitopes; Humans; Interferon alpha-2; Interferon-alpha; Mercaptoethanol; Peptides; Protein Binding; Recombinant Proteins; Urea | 2000 |
The native metastable fold of C1-inhibitor is stabilized by disulfide bonds.
Topics: alpha 1-Antitrypsin; Binding Sites; Complement C1 Inactivator Proteins; Complement C1 Inhibitor Protein; Cysteine; Cysteine Proteinase Inhibitors; Disulfides; Dithiothreitol; Hot Temperature; Humans; Oxidation-Reduction; Protein Conformation; Protein Folding; Protein Structure, Secondary; Urea | 2000 |
Mouse T-cell antigen rt6.1 has thiol-dependent NAD glycohydrolase activity.
Topics: Adenosine Diphosphate Ribose; ADP Ribose Transferases; Animals; Antigens, Differentiation, T-Lymphocyte; COS Cells; Cysteine; Dithiothreitol; Escherichia coli; Ethylmaleimide; Histocompatibility Antigens; Hydrolysis; Kinetics; Macrophages, Peritoneal; Membrane Glycoproteins; Mice; Mice, Inbred BALB C; Mutation; NAD; NAD+ Nucleosidase; Poly(ADP-ribose) Polymerases; Recombinant Fusion Proteins; Sulfhydryl Compounds; T-Lymphocytes; Transfection | 2000 |
Inhibition of cholesteryl ester transfer protein by substituted dithiobisnicotinic acid dimethyl ester: involvement Of a critical cysteine.
Topics: Amino Acid Substitution; Base Sequence; Binding Sites; Carrier Proteins; Cholesterol Ester Transfer Proteins; Chromatography, Liquid; Cysteine; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Glycoproteins; Humans; Kinetics; Mass Spectrometry; Molecular Sequence Data; Molecular Structure; Mutagenesis, Site-Directed; Nicotinic Acids; Oxidation-Reduction; Peptide Fragments; Protein Binding; Sulfides; Time Factors | 2000 |
Effects of metal ions on the activity of protein tyrosine phosphatase VHR: highly potent and reversible oxidative inactivation by Cu2+ ion.
Topics: Binding Sites; Copper; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Dual Specificity Phosphatase 3; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Glutathione; Hydrogen Peroxide; Ions; Mass Spectrometry; Models, Chemical; Mutagenesis, Site-Directed; Oxygen; Protein Tyrosine Phosphatases; Time Factors; Zinc | 2000 |
Reversible inactivation of AT(2) angiotensin II receptor from cysteine-disulfide bond exchange.
Topics: Alanine; Amino Acid Sequence; Amino Acid Substitution; Animals; Binding, Competitive; COS Cells; Cysteine; Disulfides; Dithiothreitol; Molecular Sequence Data; Mutation; Protein Conformation; Rats; Rats, Inbred SHR; Receptor, Angiotensin, Type 1; Receptor, Angiotensin, Type 2; Receptors, Angiotensin; Recombinant Proteins | 2000 |
Modification of cysteine residues in the ChlI and ChlH subunits of magnesium chelatase results in enzyme inactivation.
Topics: Adenosine Triphosphatases; Bacterial Proteins; Cysteine; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Lyases | 2000 |
Oxidative regulation of fatty acid-induced tau polymerization.
Topics: Actin Cytoskeleton; Alzheimer Disease; Antioxidants; Buffers; Butylated Hydroxytoluene; Cysteine; Dithiothreitol; Fatty Acids, Nonesterified; Humans; Microscopy, Electron; Mutagenesis, Site-Directed; Oxidation-Reduction; Oxidative Stress; Polymers; tau Proteins | 2000 |
Intracellular folding pathway of the cystine knot-containing glycoprotein hormone alpha-subunit.
Topics: Amino Acid Motifs; Cell Line; Chorionic Gonadotropin, beta Subunit, Human; Cysteine; Cystine; Dithiothreitol; Glycoprotein Hormones, alpha Subunit; Humans; Intracellular Fluid; Mutagenesis, Site-Directed; Peptide Fragments; Protein Folding; Protein Structure, Secondary; Protein Structure, Tertiary; Reducing Agents | 2001 |
Suppression of urease levels in Streptococcus salivarius by cysteine, related compounds and by sulfide.
Topics: Bacterial Proteins; Bacteriological Techniques; Cysteamine; Cysteine; Dental Plaque; Dithiothreitol; Enzyme Inhibitors; Glutathione; Humans; Hydrogen-Ion Concentration; Hydrolysis; Mercaptoethanol; Penicillamine; Periodontal Diseases; Species Specificity; Streptococcus; Sulfides; Urease | 2000 |
Three pairs of cysteine residues mediate both redox and zn2+ modulation of the nmda receptor.
Topics: Animals; Binding Sites; Cells, Cultured; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Drug Synergism; Glycine; Histidine; Mesylates; Mutagenesis, Site-Directed; N-Methylaspartate; Oocytes; Oxidation-Reduction; Patch-Clamp Techniques; Receptors, N-Methyl-D-Aspartate; Structure-Activity Relationship; Sulfhydryl Reagents; Transfection; Xenopus; Zinc | 2001 |
The mitochondrial copper metallochaperone Cox17 exists as an oligomeric, polycopper complex.
Topics: Blotting, Western; Cation Transport Proteins; Copper; Cysteine; Dimerization; Dithiothreitol; Electron Transport Complex IV; Escherichia coli; Glutathione Transferase; Macromolecular Substances; Metalloproteins; Metallothionein; Mitochondria; Molecular Chaperones; Mutagenesis, Site-Directed; Plasmids; Protein Biosynthesis; Proteins; Recombinant Proteins; Spectrophotometry, Ultraviolet; Spectrum Analysis; X-Rays | 2001 |
Thioredoxin peroxidases of the malarial parasite Plasmodium falciparum.
Topics: Amino Acid Sequence; Animals; Antioxidants; Cloning, Molecular; Cysteine; Disulfides; Dithiothreitol; Hydrogen Peroxide; Kinetics; Molecular Sequence Data; Molecular Weight; Neoplasm Proteins; Open Reading Frames; Peroxidases; Peroxiredoxins; Plasmodium falciparum; Recombinant Proteins; Sequence Alignment; Substrate Specificity | 2001 |
Cysteine residues are involved in structure and function of melanocortin 1 receptor: Substitution of a cysteine residue in transmembrane segment two converts an agonist to antagonist.
Topics: Adrenocorticotropic Hormone; alpha-MSH; Amino Acid Sequence; Amino Acid Substitution; Animals; Binding, Competitive; COS Cells; Cyclic AMP; Cysteine; Dithiothreitol; DNA, Recombinant; Dose-Response Relationship, Drug; Humans; Iodine Radioisotopes; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Radioligand Assay; Receptors, Corticotropin; Receptors, Melanocortin; Structure-Activity Relationship; Transfection | 2001 |
Activation of class III ribonucleotide reductase by thioredoxin.
Topics: Animals; Arabidopsis; Chlamydomonas reinhardtii; Cysteine; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Enzyme Activation; Escherichia coli; Free Radicals; NADP; Protein Structure, Tertiary; Ribonucleotide Reductases; S-Adenosylmethionine; Spectrophotometry; Thioredoxins; Time Factors; Ultraviolet Rays | 2001 |
Discrimination between native and non-native disulfides by protein-disulfide isomerase.
Topics: Animals; Aprotinin; Cattle; Cysteine; Disulfides; Dithiothreitol; Kinetics; Oxidation-Reduction; Protein Disulfide-Isomerases; Protein Folding; Ribonuclease, Pancreatic; Substrate Specificity | 2001 |
The surface accessibility of the glycine receptor M2-M3 loop is increased in the channel open state.
Topics: Amino Acid Substitution; Cell Line; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Glycine; Humans; Ion Channel Gating; Kidney; Mesylates; Mutagenesis, Site-Directed; Patch-Clamp Techniques; Protein Binding; Protein Conformation; Receptors, Glycine; Reducing Agents; Reflex, Startle; Transfection | 2001 |
Mapping of contact sites in complex formation between transducin and light-activated rhodopsin by covalent crosslinking: use of a photoactivatable reagent.
Topics: Amino Acid Sequence; Animals; Azides; Binding Sites; Cattle; COS Cells; Cross-Linking Reagents; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Guanosine Diphosphate; Light; Lysine; Maleimides; Models, Molecular; Molecular Sequence Data; Mutation; Photolysis; Protein Binding; Protein Structure, Secondary; Pyridines; Rhodopsin; Sepharose; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Transducin; Trypsin; Ultraviolet Rays | 2001 |
Dithiothreitol induces the sacrificial antioxidant property of human serum albumin in a metal-catalyzed oxidation and gamma-irradiation system.
Topics: Antioxidants; Ascorbic Acid; Catalysis; Cysteine; Disulfides; Dithiothreitol; DNA; DNA Damage; Dose-Response Relationship, Drug; Dose-Response Relationship, Radiation; Electrophoresis, Polyacrylamide Gel; Gamma Rays; Glutathione; Humans; Hydrogen Peroxide; Metals; Nitrobenzoates; Serum Albumin; Sulfhydryl Compounds; Time Factors | 2001 |
Peroxynitrite-dependent activation of src tyrosine kinases lyn and hck in erythrocytes is under mechanistically different pathways of redox control.
Topics: 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; Alkylation; Cysteine; Diffusion; Dithiothreitol; Enzyme Activation; Erythrocytes; Ethylmaleimide; Glucose; Humans; Hydrogen Peroxide; Immunochemistry; Nitrates; Oxidation-Reduction; Protein Tyrosine Phosphatases; Protein-Tyrosine Kinases; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-hck; Sequence Homology; Signal Transduction; Sodium Bicarbonate; src-Family Kinases; Substrate Specificity | 2001 |
Garlic and garlic-derived compounds inhibit human squalene monooxygenase.
Topics: Cysteine; Cystine; Cytochrome P-450 Enzyme Inhibitors; Dimercaprol; Dithiothreitol; Dose-Response Relationship, Drug; Garlic; Glutathione; Humans; Organoselenium Compounds; Oxygenases; Plant Extracts; Plants, Medicinal; Squalene Monooxygenase | 2001 |
Role of cysteine-291 and cysteine-322 in the polymerization of human tau into Alzheimer-like filaments.
Topics: Alzheimer Disease; Biopolymers; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Humans; Microscopy, Electron; Protein Isoforms; tau Proteins | 2001 |
Mercuric ion attenuates nuclear factor-kappaB activation and DNA binding in normal rat kidney epithelial cells: implications for mercury-induced nephrotoxicity.
Topics: Animals; Binding, Competitive; Biological Transport; Cell Line; Cell Nucleus; Cysteine; Dithiothreitol; DNA; DNA-Binding Proteins; Epithelial Cells; Escherichia coli; Glutathione; I-kappa B Proteins; Kidney; Kidney Diseases; Lipopolysaccharides; Luciferases; Mercury; NF-kappa B; NF-KappaB Inhibitor alpha; Phosphorylation; Rats; Transcription, Genetic; Transfection | 2001 |
Novel intra- and inter-molecular sulfinamide bonds in S100A8 produced by hypochlorite oxidation.
Topics: Amino Acid Sequence; Antigens, Differentiation; Aspartic Acid; Binding Sites; Calcium-Binding Proteins; Calgranulin A; Chromatography, High Pressure Liquid; Cyclohexanones; Cysteine; Dimerization; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Hydrogen Peroxide; Hypochlorous Acid; Lysine; Models, Chemical; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Neutrophils; Oxygen; Peptides; Peroxidase; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfenic Acids; Sulfur; Tetradecanoylphorbol Acetate; Time Factors | 2001 |
Detection of disulfide bonds in bovine brain tubulin and their role in protein folding and microtubule assembly in vitro: a novel disulfide detection approach.
Topics: Ammonium Sulfate; Animals; Brain Chemistry; Cattle; Colchicine; Cysteine; Disulfides; Dithiothreitol; Microtubules; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Reducing Agents; Spectrometry, Fluorescence; Tryptophan; Tubulin; Urea | 2001 |
Mechanism of integrin activation by disulfide bond reduction.
Topics: Alkylating Agents; Amino Acid Sequence; Cysteine; Disulfides; Dithiothreitol; Humans; Hydrolysis; Iodoacetamide; Ligands; Molecular Sequence Data; Oxidation-Reduction; Peptide Mapping; Platelet Glycoprotein GPIIb-IIIa Complex; Protein Binding; Protein Conformation; Reducing Agents; Serine Endopeptidases; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2001 |
Redox modulation of T-type calcium channels in rat peripheral nociceptors.
Topics: Analysis of Variance; Animals; Calcium Channels, T-Type; Cell Line; Cells, Cultured; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Female; Ganglia, Spinal; Hot Temperature; Humans; Hyperalgesia; Membrane Potentials; Neurons; Neurons, Afferent; Nociceptors; Oxidation-Reduction; Pain; Patch-Clamp Techniques; Rats; Rats, Sprague-Dawley; Recombinant Proteins; Skin; Transfection | 2001 |
Thiol-disulfide exchange between nuclear-encoded and chloroplast-encoded subunits of pea acetyl-CoA carboxylase.
Topics: Acetyl-CoA Carboxylase; Adaptation, Biological; Amino Acid Sequence; Cell Nucleus; Chloroplasts; Cysteine; Cystine; Dithiothreitol; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Light; Molecular Sequence Data; Oxidation-Reduction; Pisum sativum; Protein Subunits; Sequence Homology, Amino Acid | 2001 |
Redox regulation of the DNA repair function of the human AP endonuclease Ape1/ref-1.
Topics: Apurinic Acid; Carbon-Oxygen Lyases; Cysteine; Diamide; Dithiothreitol; DNA Damage; DNA Repair; DNA-(Apurinic or Apyrimidinic Site) Lyase; Humans; Hydrogen Peroxide; Kinetics; Models, Biological; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Oxidants; Oxidation-Reduction; Protein Processing, Post-Translational; Recombinant Fusion Proteins; Structure-Activity Relationship; Transcription Factors | 2001 |
beta-Lactoglobulin A with N-ethylmaleimide-modified sulfhydryl residue, polymerized through intermolecular disulfide bridge on heating in the presence of dithiothreitol.
Topics: Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Hot Temperature; Lactoglobulins; Polymers; Sodium Chloride; Sulfhydryl Compounds | 2001 |
The biosynthesis of ovothiol A (N-methyl-4-mercaptohistidine). Identification of S-(4'-L-histidyl)-L-cysteine sulfoxide as an intermediate and the products of the sulfoxide lyase reaction.
Topics: Animals; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Crithidia fasciculata; Cysteine; Dipeptides; Dithiothreitol; Lyases; Magnetic Resonance Spectroscopy; Mass Spectrometry; Methylhistidines; Methyltransferases; Pyridoxal Phosphate; Sulfoxides; Time Factors | 2001 |
Cysteine-25 of adenylate kinase reacts with dithiothreitol to form an adduct upon aging of the enzyme.
Topics: Adenylate Kinase; Binding Sites; Cysteine; Dithiothreitol; Protein Conformation | 2001 |
Cytoadherence of Plasmodium falciparum-infected erythrocytes is mediated by a redox-dependent conformational fraction of CD36.
Topics: Acetylcysteine; Amino Acid Sequence; Animals; Antibodies, Monoclonal; Antigen-Antibody Reactions; CD36 Antigens; Cell Adhesion; Cell Line; Chemical Fractionation; COS Cells; Cysteine; Dithiothreitol; Endothelium, Vascular; Epitopes; Erythrocyte Aggregation; Erythrocytes; Humans; Molecular Sequence Data; Oxidation-Reduction; Plasmodium falciparum; Protein Conformation; Reducing Agents; U937 Cells | 2001 |
Self-assembly and mineralization of peptide-amphiphile nanofibers.
Topics: Bioartificial Organs; Biopolymers; Bone and Bones; Calcification, Physiologic; Calcium; Collagen; Crystallization; Cysteine; Disulfides; Dithiothreitol; Durapatite; Extracellular Matrix; Hydrogen-Ion Concentration; Micelles; Microscopy, Electron; Models, Molecular; Peptides; Phosphates; Phosphoserine; Protein Structure, Quaternary; Regeneration; Tissue Engineering | 2001 |
Proximity of two oppositely oriented reentrant loops in the glutamate transporter GLT-1 identified by paired cysteine mutagenesis.
Topics: Binding Sites; Cadmium; Cysteine; Dithiothreitol; Excitatory Amino Acid Transporter 2; HeLa Cells; Humans; Mutagenesis; Protein Conformation | 2002 |
Detection, quantitation, purification, and identification of cardiac proteins S-thiolated during ischemia and reperfusion.
Topics: Aconitate Hydratase; Animals; Biotinylation; Blotting, Western; Chromatography, High Pressure Liquid; Cysteine; Cytoskeleton; Cytosol; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); Heat-Shock Proteins; HSP27 Heat-Shock Proteins; Ischemia; Isoenzymes; Models, Chemical; Myeloma Proteins; Myocardium; Myoglobin; Neoplasm Proteins; Nucleoside-Diphosphate Kinase; Oxidative Stress; Protein Binding; Protein Kinase C; Protein Kinase C-alpha; Protein Tyrosine Phosphatase, Non-Receptor Type 1; Protein Tyrosine Phosphatases; Rats; Reperfusion; Reperfusion Injury; Sepharose; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Streptavidin; Subcellular Fractions; Sulfhydryl Compounds; Triose-Phosphate Isomerase | 2002 |
Mutation in the glucose-6-phosphate dehydrogenase gene leads to inactivation of Ku DNA end binding during oxidative stress.
Topics: Animals; Antigens, Nuclear; Blotting, Western; Cell Nucleus; CHO Cells; Chromatography, High Pressure Liquid; Cricetinae; Cysteine; Disulfides; Dithiothreitol; DNA; DNA Damage; DNA Helicases; DNA Repair; DNA-Binding Proteins; Ethanol; Glucosephosphate Dehydrogenase; Ku Autoantigen; Models, Chemical; Mutation; NAD; NADP; Nuclear Proteins; Oxidative Stress; Protein Binding; Reducing Agents; Sulfhydryl Compounds; Time Factors; Transfection | 2002 |
Conserved cysteine residues in the extracellular loop of the human P2X(1) receptor form disulfide bonds and are involved in receptor trafficking to the cell surface.
Topics: Adenosine Triphosphate; Alanine; Amino Acid Sequence; Amino Acid Substitution; Animals; Biological Transport; Biotin; Biotinylation; Conserved Sequence; Cysteine; Disulfides; Dithiothreitol; Electrophysiology; Humans; Membrane Proteins; Mercaptoethanol; Molecular Sequence Data; Mutation; Oocytes; Protein Structure, Tertiary; Receptors, Purinergic P2; Receptors, Purinergic P2X; Reducing Agents; Sequence Homology, Amino Acid; Xenopus laevis | 2002 |
The thiol sensitivity of glutathione transport in sidedness-sorted basolateral liver plasma membrane and in Oatp1-expressing HeLa cell membrane.
Topics: Animals; Biological Transport; Cell Membrane; Cysteine; Dithiothreitol; Glutathione; HeLa Cells; Humans; Kinetics; Liver; Male; Membrane Potentials; Organic Anion Transporters, Sodium-Independent; Protons; Rats; Rats, Sprague-Dawley; Sulfhydryl Compounds; Transfection | 2002 |
Modulation of ICl(Ca) in vascular smooth muscle cells by oxidizing and cysteine-reactive reagents.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Calcium; Chloride Channels; Cysteine; Diamide; Dithiothreitol; Hydrogen Peroxide; In Vitro Techniques; Membrane Potentials; Muscle, Smooth, Vascular; Oxidants; Oxidation-Reduction; Patch-Clamp Techniques; Portal Vein; Rabbits; Sulfhydryl Reagents | 2002 |
Ca(2+)-activated K+ channel inhibition by reactive oxygen species.
Topics: Animals; Calcium; Cysteine; Deferoxamine; Dithiothreitol; Female; Humans; Hydrogen Peroxide; Hydroxyl Radical; Ion Channel Gating; Iron Chelating Agents; Large-Conductance Calcium-Activated Potassium Channel alpha Subunits; Large-Conductance Calcium-Activated Potassium Channels; Lipid Bilayers; Muscle, Skeletal; Oocytes; Oxidants; Patch-Clamp Techniques; Potassium Channels, Calcium-Activated; Reactive Oxygen Species; Xenopus laevis | 2002 |
Substitution of cysteine for a conserved alanine residue in the catalytic center of type II iodothyronine deiodinase alters interaction with reducing cofactor.
Topics: Alanine; Amino Acid Substitution; Animals; Catalysis; COS Cells; Cysteine; Dithiothreitol; Genetic Vectors; Humans; Iodide Peroxidase; Iodothyronine Deiodinase Type II; Kinetics; Mutagenesis, Site-Directed; Mutation; Oxidation-Reduction; Selenocysteine; Thyroxine; Triiodothyronine | 2002 |
Mutational analysis of the conserved cysteines of the rat P2X2 purinoceptor.
Topics: Adenosine Triphosphate; Animals; Cell Line; Conserved Sequence; Cysteine; Dithiothreitol; DNA Mutational Analysis; Dose-Response Relationship, Drug; Humans; Kidney; Membrane Potentials; Mesylates; Mutagenesis, Site-Directed; Oocytes; Rats; Receptors, Purinergic P2; Receptors, Purinergic P2X2; Reducing Agents; Structure-Activity Relationship; Sulfhydryl Reagents; Xenopus; Zinc | 2002 |
Reducing requirements for hemoglobin hydrolysis by Plasmodium falciparum cysteine proteases.
Topics: Animals; Cysteine; Cysteine Endopeptidases; Dithiothreitol; Glutathione; Hemoglobins; Hydrolysis; Oxidation-Reduction; Plasmodium falciparum; Time Factors | 2002 |
Site-directed mutagenesis reveals a novel catalytic mechanism of Mycobacterium tuberculosis alkylhydroperoxidase C.
Topics: Alanine; Binding Sites; Catalysis; Chromatography, Gel; Cysteine; Disulfides; Dithiothreitol; DNA, Superhelical; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Glutamate-Ammonia Ligase; Iron; Kinetics; Models, Biological; Mutagenesis, Site-Directed; Mutation; Mycobacterium tuberculosis; Oxygen; Peroxidases; Peroxiredoxins; Plasmids; Sulfhydryl Compounds | 2002 |
Non-essential roles of cysteine residues in functional expression and redox regulatory pathways for canine glutamate/aspartate transporter based on mutagenic analysis.
Topics: Amino Acid Transport System X-AG; Animals; Biological Transport; Cell Membrane; Cloning, Molecular; COS Cells; Cysteine; Dithiothreitol; DNA, Complementary; Dogs; Glutamic Acid; Kinetics; Mercury; Microscopy, Fluorescence; Mutagenesis, Site-Directed; Mutation; Oxidation-Reduction; Oxygen; Sodium; Telencephalon; Time Factors; Transfection | 2002 |
The roles of thiols in the bacterial organomercurial lyase (MerB).
Topics: Amino Acid Sequence; Bacterial Proteins; Cysteine; Cytosol; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Activation; Ethylmaleimide; Glutathione; Hydroxymercuribenzoates; Iodoacetamide; Kinetics; Lyases; Molecular Sequence Data; Mutagenesis, Site-Directed; Organomercury Compounds; Sequence Homology, Amino Acid; Substrate Specificity; Sulfhydryl Compounds | 2002 |
Identification of cysteines involved in ligand binding to the human melatonin MT(2) receptor.
Topics: Alkylating Agents; Cysteine; Dithiothreitol; DNA, Complementary; Epitopes; Ethylmaleimide; Humans; Immunohistochemistry; Ligands; Melatonin; Microscopy, Confocal; Mutagenesis, Site-Directed; Oligopeptides; Peptides; Receptors, Cell Surface; Receptors, Cytoplasmic and Nuclear; Receptors, Melatonin; Sulfhydryl Compounds; Transfection | 2002 |
Locking the hydrophobic loop 262-274 to G-actin surface by a disulfide bridge prevents filament formation.
Topics: Actin Cytoskeleton; Actins; Alanine; Cross-Linking Reagents; Cysteine; Disulfides; Dithiothreitol; Hydrophobic and Hydrophilic Interactions; Leucine; Magnesium Chloride; Mutagenesis, Site-Directed; Oxidation-Reduction; Peptide Fragments; Phalloidine; Polymers; Protein Engineering; Protein Structure, Tertiary; Saccharomyces cerevisiae Proteins | 2002 |
Inactivation of annexin II tetramer by S-nitrosoglutathione.
Topics: Animals; Annexin A2; Calcium; Cattle; Chromaffin Granules; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Ethylmaleimide; Glutathione; In Vitro Techniques; Liposomes; Lung; Membrane Fusion; Membranes, Artificial; Nitrates; Nitric Oxide Donors; Nitrites; Nitroprusside; Protein Conformation; S-Nitrosoglutathione | 2002 |
Comparative surface accessibility of a pore-lining threonine residue (T6') in the glycine and GABA(A) receptors.
Topics: Amino Acid Sequence; Animals; Cell Line; Copper; Cysteine; Disulfides; Dithiothreitol; Ethyl Methanesulfonate; GABA Antagonists; Glycine; Humans; Indicators and Reagents; Mesylates; Molecular Sequence Data; Mutation; Oocytes; Patch-Clamp Techniques; Phenanthrolines; Picrotoxin; Protein Subunits; Rats; Receptors, GABA-A; Receptors, Glycine; Sequence Alignment; Statistics as Topic; Sulfhydryl Reagents; Threonine; Xenopus laevis | 2002 |
Cloning of the large subunit of replication protein A (RPA) from yeast Saccharomyces cerevisiae and its DNA binding activity through redox potential.
Topics: Base Sequence; Cloning, Molecular; Cysteine; Dithiothreitol; DNA Replication; DNA-Binding Proteins; DNA, Fungal; DNA, Single-Stranded; Electrophoretic Mobility Shift Assay; Escherichia coli; Humans; Oxidation-Reduction; Protein Subunits; Recombinant Proteins; Replication Protein A; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Zinc Fingers | 2002 |
S-nitrosylation of dimethylarginine dimethylaminohydrolase regulates enzyme activity: further interactions between nitric oxide synthase and dimethylarginine dimethylaminohydrolase.
Topics: Amidohydrolases; Amino Acid Motifs; Amino Acid Sequence; Animals; Catalytic Domain; Cell Line; Cysteine; Dithiothreitol; Enzyme Inhibitors; Humans; In Vitro Techniques; Mice; Models, Molecular; Nitric Oxide; Nitric Oxide Donors; Nitric Oxide Synthase; Nitric Oxide Synthase Type II; Pseudomonas aeruginosa | 2002 |
Activation of glucuronidation through reduction of a disulfide bond in rat UDP-glucuronosyltransferase 1A6.
Topics: Animals; Biotransformation; COS Cells; Cysteine; Disulfides; Dithiothreitol; Enzyme Inhibitors; Ethylmaleimide; Glucuronides; Glucuronosyltransferase; Isoenzymes; Male; Maleimides; Microsomes, Liver; Nitrophenols; Oxidation-Reduction; Rats; Rats, Gunn; Rats, Wistar; Reducing Agents | 2002 |
Cysteine is exported from the Escherichia coli cytoplasm by CydDC, an ATP-binding cassette-type transporter required for cytochrome assembly.
Topics: Adenosine Triphosphate; ATP-Binding Cassette Transporters; Biological Transport; Cell Membrane; Cell Movement; Cysteine; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Lactose; Mutation; Oxidation-Reduction; Penicillin G; Periplasm; Phenotype; Protein Structure, Tertiary; Protein Transport; Subcellular Fractions; Time Factors | 2002 |
Structural and functional role of the extracellular s5-p linker in the HERG potassium channel.
Topics: Amino Acid Substitution; Animals; Cation Transport Proteins; Cysteine; Dithiothreitol; Ether-A-Go-Go Potassium Channels; Ion Channel Gating; Membrane Potentials; Mesylates; Models, Molecular; Mutagenesis, Site-Directed; Oocytes; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Voltage-Gated; Protein Structure, Tertiary; Structure-Activity Relationship; Xenopus laevis | 2002 |
20 S proteasome from Saccharomyces cerevisiae is responsive to redox modifications and is S-glutathionylated.
Topics: Bridged Bicyclo Compounds; Cell Survival; Chelating Agents; Cysteine; Cysteine Endopeptidases; Dithiothreitol; Fluorescent Dyes; Glutathione; Hydrogen Peroxide; Multienzyme Complexes; Oxidants; Oxidation-Reduction; Pentetic Acid; Proteasome Endopeptidase Complex; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Signal Transduction; Sulfenic Acids; Sulfhydryl Compounds; Sulfhydryl Reagents | 2003 |
Nitric oxide-mediated inhibition of Hdm2-p53 binding.
Topics: Amino Acid Sequence; Binding Sites; Cysteine; Dithiothreitol; Enzyme-Linked Immunosorbent Assay; Glutathione; Glutathione Transferase; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Neoplasm Proteins; Nuclear Proteins; Protein Binding; Protein Conformation; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-mdm2; Recombinant Fusion Proteins; Sequence Homology, Amino Acid; Triazenes; Tumor Suppressor Protein p53 | 2002 |
Cross-linking and amyloid formation by N- and C-terminal cysteine derivatives of human apolipoprotein C-II.
Topics: Amyloid; Apolipoprotein C-II; Apolipoproteins C; Cross-Linking Reagents; Cysteine; Dimerization; Disulfides; Dithiothreitol; Humans; Microscopy, Electron; Mutagenesis, Site-Directed; Peptide Fragments | 2002 |
Disulfide bond-mediated dimerization of HLA-G on the cell surface.
Topics: Amino Acid Substitution; Antigens, Surface; Choriocarcinoma; Cysteine; Cystine; Dimerization; Dithiothreitol; Female; Genes, MHC Class II; Histocompatibility Antigens Class I; HLA Antigens; HLA-G Antigens; Humans; Molecular Weight; Oxidation-Reduction; Pregnancy; Protein Interaction Mapping; Receptors, Immunologic; Receptors, KIR; Receptors, KIR2DL4; Recombinant Fusion Proteins; Transfection; Tumor Cells, Cultured; Uterine Neoplasms | 2002 |
Familial amyotrophic lateral sclerosis mutants of copper/zinc superoxide dismutase are susceptible to disulfide reduction.
Topics: Amino Acid Substitution; Animals; Binding Sites; Cysteine; Disulfides; Dithiothreitol; Humans; Iodoacetamide; Kinetics; Mice; Motor Neuron Disease; Mutagenesis, Site-Directed; Mutation; Organ Specificity; Oxidation-Reduction; Protein Structure, Secondary; Recombinant Proteins; Superoxide Dismutase | 2003 |
Coordinated nonvectorial folding in a newly synthesized multidomain protein.
Topics: Cysteine; Dithiothreitol; Endoplasmic Reticulum; Epitopes; Glycosylation; Golgi Apparatus; HeLa Cells; Humans; Ligands; Molecular Weight; Oxidation-Reduction; Precipitin Tests; Protein Binding; Protein Biosynthesis; Protein Denaturation; Protein Folding; Protein Structure, Tertiary; Protein Transport; Receptors, LDL | 2002 |
Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications fpr metabolic regulation and bioactivity.
Topics: Adipocytes; Adiponectin; Amino Acid Sequence; Animals; Blood Glucose; Cell Line; Chromatography, Gel; Cysteine; Dimerization; Disulfides; Dithiothreitol; Female; Glucose; Hepatocytes; Humans; Hydrogen-Ion Concentration; Immunoblotting; Insulin; Intercellular Signaling Peptides and Proteins; Ligands; Male; Mice; Mice, Inbred C57BL; Microscopy, Electron; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Oxidoreductases; Protein Structure, Tertiary; Proteins; Rats; Recombinant Proteins; Sequence Homology, Amino Acid; Sex Factors; Structure-Activity Relationship; Temperature; Time Factors; Transfection | 2003 |
Role of an intrasubunit disulfide in the association state of the cytosolic homo-oligomer methionine adenosyltransferase.
Topics: Animals; Chromatography; Chromatography, Gel; Crystallography, X-Ray; Cysteine; Cytosol; Dimerization; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Glutathione; Glutathione Transferase; Kinetics; Liver; Mass Spectrometry; Methionine Adenosyltransferase; Mutation; Protein Binding; Protein Folding; Protein Structure, Tertiary; Rats; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Thermodynamics; Time Factors; Trypsin | 2003 |
Sulfhydryl modulation of K+ channels in rat ventricular myocytes.
Topics: Animals; Cysteine; Dithiothreitol; Electric Conductivity; Glutathione Disulfide; Heart Ventricles; Male; Myocytes, Cardiac; Potassium Channel Blockers; Potassium Channels; Rats; Rats, Sprague-Dawley; Temperature; Ventricular Function | 2002 |
An opsin mutant with increased thermal stability.
Topics: Amino Acid Sequence; Animals; Asparagine; Aspartic Acid; Cattle; COS Cells; Cross-Linking Reagents; Cysteine; Disulfides; Dithiothreitol; Light; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Denaturation; Recombinant Proteins; Retinaldehyde; Rhodopsin; Thermodynamics; Transducin; Transfection | 2003 |
Reduced glutathione is required for pertussis toxin secretion by Bordetella pertussis.
Topics: Animals; Bordetella pertussis; CHO Cells; Cricetinae; Cysteine; Dithiothreitol; Glutathione; Operon; Oxidation-Reduction; Pertussis Toxin; Transcription, Genetic | 2003 |
On the conformation of the COOH-terminal domain of the large mechanosensitive channel MscL.
Topics: Amino Acid Sequence; Amino Acid Substitution; Biomarkers; Cell Line; Cell Survival; Computer Simulation; Cysteine; Cytoplasm; Dithiothreitol; Dose-Response Relationship, Drug; Electric Conductivity; Escherichia coli Proteins; Hydrogen Peroxide; Ion Channel Gating; Ion Channels; Models, Molecular; Molecular Conformation; Mutation; Osmotic Pressure; Oxidants; Oxygen; Protein Structure, Tertiary; Reducing Agents | 2003 |
Schistosoma japonicum: effect of artemether on glutathione S-transferase and superoxide dismutase.
Topics: Animals; Artemether; Artemisinins; Cysteine; Dithiothreitol; Female; Glutathione Transferase; Male; Mice; Schistosoma japonicum; Schistosomiasis japonica; Schistosomicides; Sesquiterpenes; Superoxide Dismutase | 2002 |
Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method.
Topics: Agrobacterium tumefaciens; Cotyledon; Culture Techniques; Cysteine; Dithiothreitol; DNA, Bacterial; Genetic Complementation Test; Genetic Vectors; Glycine max; Hygromycin B; Phenotype; Plants, Genetically Modified; Thiosulfates; Transfection | 2003 |
Modulation of extracellular homocysteine concentration in human cell lines.
Topics: Acetylcysteine; Amino Acid Transport Systems; Carcinoma, Hepatocellular; Copper; Cysteine; Dithiothreitol; Extracellular Fluid; HeLa Cells; Homocysteine; Humans; Oxidation-Reduction; Sulfhydryl Compounds; Sulfonic Acids; Tumor Cells, Cultured | 2003 |
Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1.
Topics: Amino Acid Sequence; Archaeal Proteins; Binding Sites; Cystathionine; Cysteine; Cysteine Synthase; Desulfurococcaceae; Dithiothreitol; Enzyme Activation; Enzyme Stability; Homocysteine; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Sequence Homology, Amino Acid; Serine; Substrate Specificity; Sulfides; Temperature | 2003 |
Inhibition of LPS-induced nitric oxide production in RAW cells by radioprotective thiols.
Topics: Acetylcysteine; Amifostine; Animals; Cell Line; Cysteine; Disulfiram; Dithiothreitol; Lipopolysaccharides; Macrophages; Mercaptoethylamines; Mice; Nitric Oxide; Radiation-Protective Agents; Sulfhydryl Compounds | 2003 |
Proteolytic gut activities in the rice water weevil, Lissorhoptrus brevirostris Suffrian (Coleoptera: Curculionidae).
Topics: Animals; Coleoptera; Cysteine; Digestive System; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Endopeptidases; Enzyme Activation; Enzyme Inhibitors; Hydrogen-Ion Concentration; Larva; Protease Inhibitors; Substrate Specificity | 2003 |
O2-specific regulation of the ferrous heme-based sensor kinase FixL from Sinorhizobium meliloti and its aberrant inactivation in the ferric form.
Topics: Bacterial Proteins; Carbon Monoxide; Cysteine; Dithiothreitol; Down-Regulation; Ferric Compounds; Ferrous Compounds; Hemeproteins; Histidine Kinase; Ligands; Nitric Oxide; Oxidation-Reduction; Oxygen; Phosphorylation; Sinorhizobium meliloti; Sulfites | 2003 |
The crystal structure of augmenter of liver regeneration: A mammalian FAD-dependent sulfhydryl oxidase.
Topics: Amino Acid Sequence; Animals; Crystallography, X-Ray; Cysteine; Dithiothreitol; Flavin-Adenine Dinucleotide; Models, Molecular; Molecular Structure; Mutagenesis, Site-Directed; Oxidation-Reduction; Oxidoreductases; Protein Conformation; Protein Structure, Quaternary; Proteins; Rats; Sequence Alignment | 2003 |
Essential 110Cys in active site of membrane-associated prostaglandin E synthase-2.
Topics: Amino Acid Sequence; Binding Sites; Catalytic Domain; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Glutaredoxins; Humans; Intramolecular Oxidoreductases; Kinetics; Leucine; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Oxidation-Reduction; Oxidative Stress; Oxidoreductases; Prostaglandin-E Synthases; Protein Binding; Protein Structure, Tertiary; Proteins; Serine; Thioctic Acid; Thioredoxins | 2003 |
Factors affecting the microclimate pH of the rat jejunum in ringer bicarbonate buffer.
Topics: Amiloride; Animals; Bicarbonates; Buffers; Cysteine; Dithiothreitol; Glucose; Hydrogen-Ion Concentration; In Vitro Techniques; Intestinal Mucosa; Isotonic Solutions; Jejunum; Male; Microelectrodes; Rats; Rats, Wistar; Ringer's Solution; Sodium Azide; Sodium-Hydrogen Exchangers | 2003 |
Inhibition of Azotobacter vinelandii rhodanese by NO-donors.
Topics: Azotobacter vinelandii; Catalytic Domain; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Kinetics; Models, Molecular; Nitric Oxide; Nitric Oxide Donors; Nitrogen; Protein Structure, Tertiary; Recombinant Proteins; Spectrophotometry; Temperature; Thiosulfate Sulfurtransferase; Time Factors | 2003 |
Reduction of pentavalent antimony by trypanothione and formation of a binary and ternary complex of antimony(III) and trypanothione.
Topics: Antimony; Antiprotozoal Agents; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Glutathione; Hydrogen-Ion Concentration; Kinetics; Magnetic Resonance Spectroscopy; Molecular Structure; Oxidation-Reduction; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry, Ultraviolet; Spermidine | 2003 |
Irreversible inactivation of protein kinase C isozymes by thiol-reactive peptide substrate analogs.
Topics: Binding Sites; Cysteine; Disulfides; Dithiothreitol; Isoenzymes; Peptides; Protein Kinase C; Sulfhydryl Compounds | 2003 |
EI-1941-1 and -2, novel interleukin-1beta converting enzyme inhibitors produced by Farrowia sp. E-1941. I. Biochemical characterization of EI-1941-1 and -2.
Topics: Cyclohexanones; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Drug Stability; Epoxy Compounds; Glutathione; Humans; Interleukin-1; Serpins; Viral Proteins | 2003 |
Juxtaposition of the two distal CX3C motifs via intrachain disulfide bonding is essential for the folding of Tim10.
Topics: Amino Acid Motifs; Circular Dichroism; Cysteine; Detergents; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Hydrogen-Ion Concentration; Isoelectric Focusing; Mass Spectrometry; Membrane Proteins; Membrane Transport Proteins; Mitochondria; Mitochondrial Membrane Transport Proteins; Mitochondrial Precursor Protein Import Complex Proteins; Mitochondrial Proteins; Models, Biological; Molecular Chaperones; Mutation; Protein Binding; Protein Folding; Protein Structure, Secondary; Recombinant Fusion Proteins; Saccharomyces cerevisiae Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds; Time Factors; Trypsin | 2003 |
A constitutively activated mutant of human soluble guanylyl cyclase (sGC): implication for the mechanism of sGC activation.
Topics: Animals; Cell Line; Cysteine; Detergents; Dithiothreitol; DNA, Complementary; Dose-Response Relationship, Drug; Enzyme Activation; Genetic Vectors; Guanylate Cyclase; Heme; Hemin; Humans; Insecta; Iron; Ligands; Models, Chemical; Mutation; Nitric Oxide; Protein Binding; Protein Conformation; Receptors, Cytoplasmic and Nuclear; Soluble Guanylyl Cyclase; Spectrophotometry; Transfection; Tumor Cells, Cultured | 2003 |
On-chip protein sample desalting and preparation for direct coupling with electrospray ionization mass spectrometry.
Topics: Adsorption; Cysteine; Cytochromes c; Dithiothreitol; Indicators and Reagents; Insulin; Iodoacetamide; Lactoglobulins; Propranolol; Protein Array Analysis; Proteins; Salts; Spectrometry, Mass, Electrospray Ionization; Urea | 2003 |
Single-molecule covalent chemistry with spatially separated reactants.
Topics: Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; Hemolysin Proteins; Hydrogen-Ion Concentration; Kinetics; Membranes, Artificial; Molecular Conformation; Sulfhydryl Reagents | 2003 |
Highly conserved cysteines of mouse core 2 beta1,6-N-acetylglucosaminyltransferase I form a network of disulfide bonds and include a thiol that affects enzyme activity.
Topics: Amino Acid Sequence; Animals; Bacteriophage T4; Binding Sites; Cations; Cell Adhesion; Chymotrypsin; Conserved Sequence; Cysteine; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Glucosyltransferases; Kinetics; Mice; Models, Molecular; Molecular Sequence Data; Mutation; N-Acetylglucosaminyltransferases; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase; Polysaccharides; Protein Folding; Protein Isoforms; Recombinant Proteins; Reducing Agents; Sequence Homology, Amino Acid; Serine; Serine Endopeptidases; Sulfhydryl Compounds; Time Factors; Trypsin | 2003 |
Organochalcogens effects on delta-aminolevulinate dehydratase activity from human erythrocytic cells in vitro.
Topics: Antioxidants; Azoles; Benzene Derivatives; Cysteine; Disulfides; Dithiothreitol; Drug Interactions; Erythrocytes; Glutathione Transferase; Humans; Isoindoles; Organometallic Compounds; Organoselenium Compounds; Porphobilinogen Synthase; Zinc | 2003 |
Role of disulfide bonds in Acrp30/adiponectin structure and signaling specificity. Different oligomers activate different signal transduction pathways.
Topics: Adiponectin; Alanine; Amino Acid Sequence; Animals; Blotting, Western; Cell Line; Chromatography, Gel; Cryoelectron Microscopy; Cysteine; Dimerization; Disulfides; Dithiothreitol; Genes, Reporter; Humans; Intercellular Signaling Peptides and Proteins; Ligands; Luciferases; Mice; Molecular Sequence Data; Muscles; Mutagenesis, Site-Directed; NF-kappa B; Protein Conformation; Protein Isoforms; Proteins; Sequence Homology, Amino Acid; Signal Transduction | 2003 |
A cytochrome b562 variant with a c-type cytochrome CXXCH heme-binding motif as a probe of the Escherichia coli cytochrome c maturation system.
Topics: Amino Acid Motifs; Cysteine; Cytochrome b Group; Cytochromes c; Disulfides; Dithionite; Dithiothreitol; Escherichia coli; Escherichia coli Proteins; Heme; Hemin; Mutation; Plasmids; Protein Binding; Protein Disulfide-Isomerases; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry; Subcellular Fractions | 2003 |
Oxidative inactivation of purified plasma membrane Ca2+-ATPase by hydrogen peroxide and protection by calmodulin.
Topics: Animals; Calcium-Transporting ATPases; Calmodulin; Cation Transport Proteins; Cattle; Cysteine; Dithiothreitol; Enzyme Activation; Erythrocyte Membrane; Histidine; Humans; Hydrogen Peroxide; Leucine; Oxidation-Reduction; Plasma Membrane Calcium-Transporting ATPases; Protein Conformation; Serine; Spectrometry, Fluorescence; Tyrosine; Valine | 2003 |
Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+-dependent phospho-alpha-glucosidase(s).
Topics: alpha-Glucosidases; Amino Acid Sequence; Catalysis; Cell-Free System; Chromatography; Chromatography, High Pressure Liquid; Clostridium; Cysteine; Disaccharides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Fusobacterium; Genome, Bacterial; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Manganese; Molecular Sequence Data; Mutagenesis, Site-Directed; NAD; Operon; Protein Isoforms; Proteome; Recombinant Proteins; Sequence Homology, Amino Acid; Spectrometry, Mass, Electrospray Ionization; Spectrophotometry; Substrate Specificity; Sucrose; Time Factors | 2004 |
Formation of disulfide bond in p53 correlates with inhibition of DNA binding and tetramerization.
Topics: Binding Sites; Blotting, Western; Chromatography, Gel; Cysteine; Disulfides; Dithionitrobenzoic Acid; Dithiothreitol; DNA; DNA-Binding Proteins; Electrophoretic Mobility Shift Assay; GADD45 Proteins; Humans; Intracellular Signaling Peptides and Proteins; Maleimides; Microscopy, Atomic Force; Models, Molecular; Molecular Weight; Nitrobenzoates; Nuclear Proteins; Oligonucleotides; Oxidation-Reduction; Polyethylene Glycols; Promoter Regions, Genetic; Protein Binding; Protein Structure, Quaternary; Proteins; Proto-Oncogene Proteins; Proto-Oncogene Proteins c-mdm2; Recombinant Proteins; Sulfenic Acids; Sulfhydryl Compounds; Sulfinic Acids; Sulfonic Acids; Tumor Suppressor Protein p53 | 2003 |
Effect of reducing and oxidizing agents and pH on malt endoproteolytic activities and brewing mashes.
Topics: Cysteine; Diamide; Dithiothreitol; Edible Grain; Endopeptidases; Food Handling; Hordeum; Hot Temperature; Hydrogen Peroxide; Hydrogen-Ion Concentration; Hydrolysis; Mercaptoethanol; Oxidants; Protease Inhibitors; Reducing Agents | 2003 |
The orientation and molecular movement of a k(+) channel voltage-sensing domain.
Topics: Animals; Cysteine; Dithiothreitol; Drosophila; Drosophila Proteins; Electric Conductivity; Hydrogen Peroxide; Indicators and Reagents; Membrane Potentials; Mesylates; Models, Biological; Molecular Biology; Mutation; Oocytes; Oxidants; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Voltage-Gated; Protein Conformation; Protein Structure, Tertiary; Rhodamines; Sequence Alignment; Shaker Superfamily of Potassium Channels; Structure-Activity Relationship; Time Factors; Xenopus laevis | 2003 |
Regulation of annexin A2 by reversible glutathionylation.
Topics: Actins; Animals; Annexin A2; Biotinylation; Cattle; Cysteine; Diamide; Dithiothreitol; Glutaredoxins; Glutathione; Homeostasis; Hydrogen Peroxide; Liposomes; Mass Spectrometry; Oxidants; Oxidation-Reduction; Oxidative Stress; Oxidoreductases; Phospholipids; Proteins; Structure-Activity Relationship; Tumor Necrosis Factor-alpha | 2004 |
Peroxynitrite irreversibly inactivates the human xenobiotic-metabolizing enzyme arylamine N-acetyltransferase 1 (NAT1) in human breast cancer cells: a cellular and mechanistic study.
Topics: Arylamine N-Acetyltransferase; Breast Neoplasms; Cysteine; Dithiothreitol; Enzyme Inhibitors; Glutathione; Humans; Isoenzymes; Kinetics; Molsidomine; Oxidation-Reduction; Peroxynitrous Acid; Recombinant Proteins; Tumor Cells, Cultured | 2004 |
Identification of a redox-regulated chaperone network.
Topics: Animals; Cattle; Citrate (si)-Synthase; Cysteine; Dimerization; Disulfides; Dithiothreitol; Fluorescence Polarization; Heat-Shock Proteins; Luciferases; Models, Biological; Molecular Chaperones; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Folding; Protein Processing, Post-Translational; Reducing Agents; Serum Albumin, Bovine; Substrate Specificity; Swine; Temperature; Time Factors | 2004 |
Preparation and utilization of a reagent for the isolation and purification of low-molecular-mass thiols.
Topics: Chromatography, High Pressure Liquid; Chromatography, Thin Layer; Cysteine; Disaccharides; Dithiothreitol; Glutathione; Glycopeptides; Inositol; Mycobacterium smegmatis; Naphthalenes; Pyrazoles; Sulfhydryl Compounds; Sulfhydryl Reagents | 2004 |
Direct observation of covalent adducts with Cys34 of human serum albumin using mass spectrometry.
Topics: Cysteine; Dithiothreitol; Glutathione; Gold Compounds; Humans; Iodoacetamide; Serum Albumin; Spectrometry, Mass, Electrospray Ionization | 2004 |
The outer pore of the glutamate receptor channel has 2-fold rotational symmetry.
Topics: 6-Cyano-7-nitroquinoxaline-2,3-dione; Amino Acid Motifs; Amino Acid Substitution; Animals; Binding Sites; Cadmium; Chelating Agents; Conserved Sequence; Copper; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Drug Interactions; Excitatory Amino Acid Agonists; Excitatory Amino Acid Antagonists; Extracellular Space; Glutamic Acid; Ion Channel Gating; Kinetics; Ligands; Membrane Potentials; Microinjections; Models, Molecular; Oocytes; Patch-Clamp Techniques; Phenanthrolines; Protein Structure, Tertiary; Receptors, AMPA; Sulfhydryl Compounds; Unithiol; Xenopus | 2004 |
Distribution and characterization of hemolytic activity by an oral anaerobe from the Streptococcus milleri group.
Topics: Adult; Animals; Bacterial Toxins; Child; Cholesterol; Chymotrypsin; Cysteine; Dithiothreitol; Endopeptidase K; Glutathione; Hemolysin Proteins; Humans; Mercaptoethanol; Mouth; Pronase; Protease Inhibitors; Rabbits; Streptococcus milleri Group; Subtilisin; Trypsin | 2004 |
About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis.
Topics: Alkylation; Animals; Cattle; Cysteine; Cystine; Disulfides; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Gels; Hydrogen Peroxide; Hydrogen-Ion Concentration; Iodoacetamide; Maleimides; Mass Spectrometry; Mitochondria; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds; Temperature; Time Factors; Water | 2004 |
2,3-Dimercaptopropanol, 2,3-dimercaptopropane-1-sulfonic acid, and meso-2,3-dimercaptosuccinic acid inhibit delta-aminolevulinate dehydratase from human erythrocytes in vitro.
Topics: Chelating Agents; Chlorides; Cysteine; Dimercaprol; Dithiothreitol; Dose-Response Relationship, Drug; Erythrocytes; Humans; In Vitro Techniques; Porphobilinogen Synthase; Reducing Agents; Succimer; Unithiol; Zinc Compounds | 2004 |
Implications of the role of reactive cystein in arginine kinase: reactivation kinetics of 5,5'-dithiobis-(2-nitrobenzoic acid)-modified arginine kinase reactivated by dithiothreitol.
Topics: Arginine Kinase; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Reactivators; Kinetics; Magnesium; Substrate Specificity | 2004 |
Catalytic inactivation of protein tyrosine phosphatase CD45 and protein tyrosine phosphatase 1B by polyaromatic quinones.
Topics: Catalysis; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Enzyme Inhibitors; Enzyme Reactivators; Humans; Hydroxylamine; Intracellular Signaling Peptides and Proteins; Iodine Radioisotopes; Leukocyte Common Antigens; Membrane Proteins; Oxidation-Reduction; Oxygen; Phenanthrenes; Phosphoproteins; Protein Tyrosine Phosphatase, Non-Receptor Type 1; Protein Tyrosine Phosphatases; Quinones; Structure-Activity Relationship; Sulfenic Acids; Sulfonic Acids | 2004 |
Direct photoaffinity labeling by dolastatin 10 of the amino-terminal peptide of beta-tubulin containing cysteine 12.
Topics: Animals; Antineoplastic Agents; Binding Sites; Brain; Cattle; Computer Simulation; Cysteine; Depsipeptides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Guanosine Diphosphate; Kinetics; Ligands; Light; Models, Chemical; Models, Molecular; Oligopeptides; Peptides; Peptides, Cyclic; Photoaffinity Labels; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Thiazoles; Time Factors; Tubulin; Ultraviolet Rays | 2004 |
Oxidative stress inhibits MEKK1 by site-specific glutathionylation in the ATP-binding domain.
Topics: Adenosine Triphosphate; Alkylation; Amino Acid Sequence; Amino Acid Substitution; Binding Sites; Catalytic Domain; Cell Line, Tumor; Cysteine; Dithiothreitol; Enzyme Inhibitors; Ethylmaleimide; Glutathione; Humans; Lymph Nodes; Male; MAP Kinase Kinase Kinase 1; Molecular Sequence Data; Mutation; Oxidants; Oxidation-Reduction; Oxidative Stress; Peptides; Prostatic Neoplasms; Protein Structure, Tertiary; Valine | 2004 |
Herbimycin A abrogates nuclear factor-kappaB activation by interacting preferentially with the IkappaB kinase beta subunit.
Topics: Benzoquinones; Catalytic Domain; Cell Line; Cells, Cultured; Cysteine; Dithiothreitol; Enzyme Inhibitors; Humans; I-kappa B Kinase; I-kappa B Proteins; Immunohistochemistry; Lactams, Macrocyclic; Mutagenesis, Site-Directed; NF-kappa B; NF-KappaB Inhibitor alpha; Phosphorylation; Protein Serine-Threonine Kinases; Quinones; Rifabutin; Signal Transduction; Transfection | 2004 |
Structural characterization of fish egg vitelline envelope proteins by mass spectrometry.
Topics: Amino Acid Sequence; Animals; Cysteine; Disulfides; Dithiothreitol; Egg Proteins; Molecular Sequence Data; Oncorhynchus mykiss; Ovum; Sequence Alignment; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2004 |
Intramolecular disulfide bonds are required for folding hydrolase B into a catalytically active conformation but not for maintaining it during catalysis.
Topics: Animals; Carboxylesterase; Carboxylic Ester Hydrolases; Cysteine; Disulfides; Dithiothreitol; Microsomes, Liver; Mutagenesis, Site-Directed; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Protein Folding; Rats; Urea | 2004 |
A stable mercury-containing complex of the organomercurial lyase MerB: catalysis, product release, and direct transfer to MerA.
Topics: Bacterial Proteins; Carbon; Catalysis; Cysteine; Dithiothreitol; Ions; Kinetics; Ligands; Lyases; Magnetic Resonance Spectroscopy; Mercury; Models, Chemical; Oxidoreductases; Protein Binding; Spectrophotometry, Atomic; Substrate Specificity; Time Factors | 2004 |
Evidence for disulfide involvement in the regulation of intramolecular autolytic processing by human adamalysin19/ADAM19.
Topics: ADAM Proteins; Animals; Catalytic Domain; Chlorocebus aethiops; COS Cells; Cysteine; Dipeptides; Disintegrins; Disulfides; Dithiothreitol; Dogs; Humans; Hydrogen-Ion Concentration; Metalloendopeptidases; Mutagenesis, Site-Directed; Protein Processing, Post-Translational; Protein Structure, Tertiary; Serine | 2004 |
H2O2-induced intermolecular disulfide bond formation between receptor protein-tyrosine phosphatases.
Topics: Animals; Catalysis; Coloring Agents; COS Cells; Cysteine; Dimerization; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Genetic Vectors; Humans; Hydrogen Peroxide; Immunoprecipitation; Mutation; Oxidation-Reduction; Oxygen; Protein Conformation; Protein Folding; Protein Structure, Tertiary; Protein Tyrosine Phosphatases; Receptor-Like Protein Tyrosine Phosphatases, Class 4; Receptors, Cell Surface; Time Factors; Transfection; Vanadates | 2004 |
Nitric oxide-induced modification of protein thiolate clusters as determined by spectral fluorescence resonance energy transfer in live endothelial cells.
Topics: Animals; Cells, Cultured; Cyclic GMP; Cysteine; Dithiothreitol; Endothelium, Vascular; Enzyme Activation; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Free Radicals; Genes, Reporter; Green Fluorescent Proteins; Guanylate Cyclase; Heme; Microscopy, Fluorescence; Nitric Oxide; Nitroso Compounds; Peptides; Protein Conformation; Proteins; Receptors, Cytoplasmic and Nuclear; Recombinant Fusion Proteins; Sheep; Signal Transduction; Soluble Guanylyl Cyclase; Spectrometry, Fluorescence; Sulfhydryl Compounds | 2004 |
Cross-linking of promoter DNA to T7 RNA polymerase does not prevent formation of a stable elongation complex.
Topics: Base Sequence; Chromatography, Gel; Cross-Linking Reagents; Cysteine; Disulfides; Dithiothreitol; DNA; DNA-Directed RNA Polymerases; Escherichia coli; Glutathione; Models, Molecular; Molecular Sequence Data; Mutation; Oligonucleotides; Oxygen; Promoter Regions, Genetic; Proteins; RNA; Transcription, Genetic; Viral Proteins | 2004 |
Mechanism of cysteine desulfurase Slr0387 from Synechocystis sp. PCC 6803: kinetic analysis of cleavage of the persulfide intermediate by chemical reductants.
Topics: Carbon-Sulfur Lyases; Catalysis; Cyanobacteria; Cysteine; Dithiothreitol; Hydrogen-Ion Concentration; Kinetics; Mercaptoethanol; Phosphines; Pyridoxal Phosphate; Reducing Agents; Spectrum Analysis; Sulfhydryl Compounds; Sulfides; Temperature | 2004 |
Human xylosyltransferase I: functional and biochemical characterization of cysteine residues required for enzymic activity.
Topics: Amino Acid Sequence; Animals; Catalysis; Cell Line; Chondroitin Sulfates; Cloning, Molecular; Cysteine; Disulfides; Dithiothreitol; Enzyme Inhibitors; Epitopes; Heparin; Heparitin Sulfate; Humans; Insecta; Maleimides; Microspheres; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Oxidation-Reduction; Pentosyltransferases; Recombinant Proteins; Solubility; UDP Xylose-Protein Xylosyltransferase; Uridine Diphosphate | 2005 |
Purification and characterization of human uroporphyrinogen III synthase expressed in Escherichia coli.
Topics: Biochemistry; Crystallography, X-Ray; Cysteine; Dithiothreitol; DNA, Complementary; Edetic Acid; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Genetic Vectors; Glycerol; Hot Temperature; Humans; Hydroxymethylbilane Synthase; Kinetics; Models, Chemical; Oxygen; Porphyrins; Sulfhydryl Compounds; Temperature; Time Factors; Uroporphyrinogen III Synthetase | 2004 |
Cys-His proteases are among the wired proteins of the cell.
Topics: Animals; Binding Sites; Biophysics; Cathepsin B; Cattle; Copper; Cysteine; Cystine; Dithiothreitol; Dose-Response Relationship, Drug; Enzyme Inhibitors; Glutathione; Histidine; Hydrogen-Ion Concentration; Ions; Iron; Lysosomes; Models, Biological; Models, Molecular; Oxidation-Reduction; Oxygen; Peptide Hydrolases; Protein Binding; Protons; Spleen; Time Factors; Zinc | 2004 |
Differential protein mobility of the gamma-aminobutyric acid, type A, receptor alpha and beta subunit channel-lining segments.
Topics: Animals; Binding Sites; Cysteine; Diazepam; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Electric Conductivity; Ethylmaleimide; gamma-Aminobutyric Acid; Inhibitory Concentration 50; Models, Molecular; Movement; Oocytes; Oxidation-Reduction; Protein Conformation; Protein Subunits; Rats; Receptors, GABA-A; RNA, Messenger; Zinc | 2005 |
Cysteine-reactive polymers synthesized by atom transfer radical polymerization for conjugation to proteins.
Topics: Cysteine; Disulfides; Dithiothreitol; Kinetics; Polyamines; Polyhydroxyethyl Methacrylate; Serum Albumin, Bovine | 2004 |
Parasite killing in Plasmodium vivax malaria by nitric oxide: implication of aspartic protease inhibition.
Topics: Animals; Aspartic Acid; Aspartic Acid Endopeptidases; Catalase; Catalytic Domain; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Erythrocytes; Gelatin; Glutamate Dehydrogenase (NADP+); Glutathione; Hydrogen-Ion Concentration; Inhibitory Concentration 50; Malaria, Vivax; Molsidomine; Nitric Oxide; Nitric Oxide Donors; Nitroprusside; Pepsin A; Pepstatins; Plasmodium; Plasmodium vivax; Protease Inhibitors; Reactive Nitrogen Species; S-Nitrosoglutathione; Time Factors; Up-Regulation | 2004 |
Biochemical and proteomics approaches to characterize topoisomerase IIalpha cysteines and DNA as targets responsible for cisplatin-induced inhibition of topoisomerase IIalpha.
Topics: Antigens, Neoplasm; Cell Division; Cisplatin; Cysteine; Disulfides; Dithiothreitol; DNA Topoisomerases, Type II; DNA-Binding Proteins; Humans; K562 Cells; Kinetics; Plasmids; Proteome; Topoisomerase II Inhibitors | 2005 |
Side chain orientation of residues lining the selectivity filter of epithelial Na+ channels.
Topics: Alanine; Amiloride; Amino Acid Sequence; Animals; Binding Sites; Cadmium; Cations; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Electrophysiology; Epithelial Sodium Channels; Mice; Models, Biological; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Oocytes; Protein Binding; Protein Structure, Tertiary; RNA, Complementary; Sequence Homology, Amino Acid; Serine; Sodium Channels; Time Factors; Xenopus | 2005 |
Intracellular thiol-mediated modulation of epithelial sodium channel activity.
Topics: Animals; Cadmium; Calcium; Cell Membrane; Chelating Agents; Copper; Cysteine; Dithiothreitol; Edetic Acid; Egtazic Acid; Electrophysiology; Epithelial Sodium Channels; Female; Hydrogen-Ion Concentration; Ions; Mesylates; Models, Biological; Mutagenesis, Site-Directed; Oocytes; Oxygen; Protein Conformation; Protein Structure, Tertiary; RNA, Complementary; Sodium; Sodium Channels; Sulfhydryl Compounds; Time Factors; Xenopus laevis; Zinc | 2005 |
[Determination of L-cysteine in enzymatic reaction mixture with pre-column derivatization by high performance liquid chromatography].
Topics: Chromatography, High Pressure Liquid; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Mercaptoethanol; Photoelectron Spectroscopy | 2004 |
Alpha-adrenergic receptor-stimulated hypertrophy in adult rat ventricular myocytes is mediated via thioredoxin-1-sensitive oxidative modification of thiols on Ras.
Topics: Animals; Catalase; Cysteine; Dicarboxylic Acids; Dithiothreitol; Enzyme Activation; Heart Ventricles; Hypertrophy; Male; MAP Kinase Kinase 1; MAP Kinase Kinase 2; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Myocytes, Cardiac; Norepinephrine; Oxidation-Reduction; Peroxidase; Phosphorylation; Protein Processing, Post-Translational; Proto-Oncogene Proteins p21(ras); Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Receptors, Adrenergic, alpha; Recombinant Fusion Proteins; Ribosomal Protein S6 Kinases, 90-kDa; Sarcomeres; Signal Transduction; Sulfhydryl Compounds; Thioredoxins | 2005 |
Reversible activation of secretory phospholipase A2 by sulfhydryl reagents.
Topics: Animals; Arsenicals; Calcium; Cysteine; Dithiothreitol; Enzyme Activation; Enzyme Inhibitors; Glutathione; Group II Phospholipases A2; Humans; PC12 Cells; Phospholipases A; Phospholipases A2; Rats; Receptors, Cell Surface; Sulfhydryl Reagents; Thimerosal; Tumor Cells, Cultured | 2005 |
Disulfide-linked dimers of human adrenaline synthesizing enzyme PNMT are catalytically active.
Topics: Binding Sites; Catalysis; Crystallization; Crystallography, X-Ray; Cysteine; Dimerization; Disulfides; Dithiothreitol; Humans; Models, Molecular; Phenylethanolamine N-Methyltransferase; Protein Conformation; Reducing Agents; Solutions; Ultracentrifugation | 2005 |
An intersubunit zinc binding site in rat P2X2 receptors.
Topics: Adenosine Triphosphate; Allosteric Site; Animals; Binding Sites; Blotting, Western; Cell Membrane; Cysteine; Dimerization; Disulfides; Dithiothreitol; DNA, Complementary; Dose-Response Relationship, Drug; Histidine; Humans; Ions; Ligands; Mesylates; Models, Biological; Mutagenesis, Site-Directed; Mutation; Oocytes; Protein Binding; Protein Structure, Tertiary; Rats; Receptors, Purinergic P2; Receptors, Purinergic P2X2; Sulfhydryl Reagents; Xenopus; Zinc | 2005 |
Microsomal UDP-glucuronyltransferase in rat liver: oxidative activation.
Topics: Animals; Ascorbic Acid; Cysteine; Dithiothreitol; Enzyme Activation; Glucuronosyltransferase; Glutathione; In Vitro Techniques; Iron; Lipid Peroxidation; Male; Microsomes, Liver; Octoxynol; Oxidation-Reduction; Rats; Rats, Sprague-Dawley; Sulfhydryl Compounds | 2005 |
Reactivities of quinone-free DsbB from Escherichia coli.
Topics: Bacterial Proteins; Benzoquinones; Cell Membrane; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Dose-Response Relationship, Drug; Escherichia coli; Kinetics; Mass Spectrometry; Membrane Proteins; Microscopy, Fluorescence; Models, Biological; Mutation; Oxidants; Oxidation-Reduction; Oxygen; Protein Folding; Protein Structure, Tertiary; Sarcosine; Time Factors; Tryptophan; Vitamin K 2 | 2005 |
The kinetic study of arginine kinase from the sea cucumber Stichopus japonicus with 5,5'-dithiobis-(2-nitrobenzoic acid).
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Arginine Kinase; Binding Sites; Catalysis; Cysteine; Dimerization; Dithionitrobenzoic Acid; Dithiothreitol; Dose-Response Relationship, Drug; Kinetics; Magnesium; Models, Chemical; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Sea Cucumbers; Spectrometry, Fluorescence; Substrate Specificity; Sulfhydryl Compounds; Time Factors; Tryptophan | 2005 |
Spontaneous thermal motion of the GABA(A) receptor M2 channel-lining segments.
Topics: Animals; Cysteine; Disulfides; Dithiothreitol; Electrophysiology; Hot Temperature; Models, Molecular; Mutagenesis; Mutation; Oocytes; Oxygen; Phenanthrolines; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Rats; Receptors, Cholinergic; Receptors, GABA-A; Time Factors; Xenopus laevis | 2005 |
Interactions of sodium selenite, glutathione, arsenic species, and omega class human glutathione transferase.
Topics: Amino Acid Sequence; Arsenicals; Cysteine; Dithiothreitol; Enzyme Inhibitors; Glutathione; Glutathione Transferase; Humans; Kinetics; Molecular Sequence Data; Oxidation-Reduction; Reactive Oxygen Species; Recombinant Proteins; Sodium Selenite; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stereoisomerism; Sulfhydryl Compounds | 2005 |
Post-translational regulation of mercaptopyruvate sulfurtransferase via a low redox potential cysteine-sulfenate in the maintenance of redox homeostasis.
Topics: Animals; Catalysis; Catalytic Domain; Cysteine; Dithiothreitol; DNA Primers; DNA, Complementary; Dose-Response Relationship, Drug; Glutathione; Homeostasis; Hydrogen Peroxide; Iodoacetates; Kinetics; Models, Chemical; Mutagenesis; NADP; Oxidants; Oxidation-Reduction; Oxidative Stress; Oxygen; Peroxidase; Protein Processing, Post-Translational; Rats; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfenic Acids; Sulfur; Sulfurtransferases; Tetrathionic Acid; Thioredoxin-Disulfide Reductase; Thioredoxins; Thiosulfate Sulfurtransferase; Time Factors | 2005 |
Amyloid-beta induces disulfide bonding and aggregation of GAPDH in Alzheimer's disease.
Topics: Aged; Aged, 80 and over; Alzheimer Disease; Amyloid beta-Peptides; Animals; Cell Line; Cell Nucleus; Cysteine; Cytoplasm; Cytosol; Detergents; Digitonin; Disulfides; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Polyacrylamide Gel; Female; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Glycolysis; Humans; Hydrogen Peroxide; Immunoblotting; Male; Mice; Mice, Transgenic; Models, Biological; Neurodegenerative Diseases; Neurons; Oxidants; Oxidation-Reduction; Polymorphism, Genetic; Protein Isoforms; Rats; Reactive Oxygen Species; Risk; Time Factors | 2005 |
ATPase activity of p97/valosin-containing protein is regulated by oxidative modification of the evolutionally conserved cysteine 522 residue in Walker A motif.
Topics: Adaptor Proteins, Vesicular Transport; Adenosine Triphosphatases; Amino Acid Motifs; Amino Acid Sequence; Animals; Cell Cycle Proteins; Cell Line; Cysteine; Cystic Fibrosis Transmembrane Conductance Regulator; Dithiothreitol; DNA, Complementary; Dose-Response Relationship, Drug; Endoplasmic Reticulum; Evolution, Molecular; Flow Cytometry; Gene Deletion; Glutathione; Humans; Intracellular Signaling Peptides and Proteins; Molecular Sequence Data; Mutation; Neurodegenerative Diseases; Oxidative Stress; Oxygen; Phenotype; Protein Binding; Proteins; Recombinant Proteins; Sequence Homology, Amino Acid; Threonine; Ubiquitin; Valosin Containing Protein | 2005 |
Metallothionein disulfides are present in metallothionein-overexpressing transgenic mouse heart and increase under conditions of oxidative stress.
Topics: Animals; Antineoplastic Agents; Blotting, Western; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Doxorubicin; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Iodoacetamide; Mass Spectrometry; Metallothionein; Mice; Mice, Transgenic; Myocardium; Oxidative Stress; Peptides; Protein Structure, Tertiary; Spectrometry, Mass, Electrospray Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Time Factors; Trypsin; Zinc | 2006 |
Different catalytic mechanisms in mammalian selenocysteine- and cysteine-containing methionine-R-sulfoxide reductases.
Topics: Amino Acid Sequence; Animals; Binding Sites; Catalysis; Conserved Sequence; Cysteine; Dithiothreitol; Evolution, Molecular; Humans; Kinetics; Methionine Sulfoxide Reductases; Molecular Sequence Data; Mutation; Oxidoreductases; Selenocysteine; Sequence Alignment; Thioredoxins | 2005 |
Enzymological characterization of FII(a), a fibrinolytic enzyme from Agkistrodon acutus venom.
Topics: Agkistrodon; Animals; Calcium; Calcium Chloride; Chlorides; Crotalid Venoms; Cysteine; Dithiothreitol; Edetic Acid; Egtazic Acid; Fibrinogen; Fibrinolysis; Fibrinolytic Agents; Magnesium Chloride; Metalloendopeptidases; Metalloproteases; Potassium; Zinc; Zinc Compounds | 2005 |
Characterization of the redox and metal binding activity of BsSco, a protein implicated in the assembly of cytochrome c oxidase.
Topics: Amino Acid Substitution; Bacterial Proteins; Calorimetry; Cations; Cations, Divalent; Cations, Monovalent; Copper; Cysteine; Cystine; Disulfides; Dithiothreitol; Electron Spin Resonance Spectroscopy; Electron Transport Complex IV; Kinetics; Membrane Proteins; Metals; Mutation; Oxidation-Reduction; Phosphines; Protein Binding; Pyridines; Recombinant Proteins; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Thermodynamics | 2005 |
Mechanistic implications of the structure of the mixed-disulfide intermediate of the disulfide oxidoreductase, 2-ketopropyl-coenzyme M oxidoreductase/carboxylase.
Topics: Acetoacetates; Binding Sites; Carbon Dioxide; Carboxy-Lyases; Crystallography, X-Ray; Cysteine; Disulfides; Dithiothreitol; Enzyme Stability; Hydrophobic and Hydrophilic Interactions; Ketone Oxidoreductases; Models, Chemical; NADP; Oxidoreductases; Substrate Specificity | 2006 |
Linker chains of the gigantic hemoglobin of the earthworm Lumbricus terrestris: primary structures of linkers L2, L3, and L4 and analysis of the connectivity of the disulfide bonds in linker L1.
Topics: Amino Acid Sequence; Animals; Aspartic Acid; Base Sequence; Binding Sites; Calcium; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; DNA, Complementary; Glutamic Acid; Heme; Hemoglobins; Histidine; Humans; Ligands; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Oligochaeta; Protein Binding; Protein Processing, Post-Translational; Protein Structure, Tertiary; Receptors, LDL; RNA, Messenger; Sequence Homology, Amino Acid; Sodium Dodecyl Sulfate; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Superoxide Dismutase | 2006 |
Variable reactivity of an engineered cysteine at position 338 in cystic fibrosis transmembrane conductance regulator reflects different chemical states of the thiol.
Topics: Alkylating Agents; Animals; Catalysis; Copper; Cysteine; Cystic Fibrosis Transmembrane Conductance Regulator; Disulfides; Dithiothreitol; Glutathione; Humans; Hydrogen Peroxide; Hydrogen-Ion Concentration; Iodoacetamide; Mercaptoethanol; Mesylates; Metals; Models, Biological; Oocytes; Oxygen; Phenotype; Protein Engineering; Sulfhydryl Compounds; Sulfinic Acids; Sulfonic Acids; Time Factors; Xenopus | 2006 |
Identification of a disulfide bridge linking the fourth and the seventh extracellular loops of the Na+/glucose cotransporter.
Topics: Animals; Cysteine; Disulfides; Dithiothreitol; DNA; Female; Fluorescent Dyes; Humans; Membrane Potentials; Models, Biological; Mutation; Oocytes; Patch-Clamp Techniques; Reducing Agents; Sodium-Glucose Transporter 1; Xenopus laevis | 2006 |
Inactivation of active and latent transforming growth factor beta by free thiols: potential redox regulation of biological action.
Topics: Bacitracin; Blotting, Western; Cysteine; Dimerization; Dithiothreitol; Dose-Response Relationship, Drug; Glutathione; Homocysteine; Humans; Oxidation-Reduction; Recombinant Proteins; Sulfhydryl Compounds; Temperature; Transforming Growth Factor beta | 2006 |
Molecular mechanism of the reduction of cysteine sulfinic acid of peroxiredoxin to cysteine by mammalian sulfiredoxin.
Topics: Adenosine Diphosphate; Adenosine Triphosphate; Animals; Catalysis; Cysteine; Disulfides; Dithiothreitol; Humans; Hydrolysis; Models, Chemical; Models, Molecular; Mutation; Oxidoreductases; Oxidoreductases Acting on Sulfur Group Donors; Peroxidases; Peroxiredoxins; Phosphates; Phosphorylation; Rats | 2006 |
Effects of biological oxidants on the catalytic activity and structure of group VIA phospholipase A2.
Topics: Amino Acid Sequence; Animals; Catalysis; Cells, Cultured; Chromatography, Liquid; Cysteine; Dithiothreitol; Group VI Phospholipases A2; Hydrogen Peroxide; Hypochlorous Acid; Methionine; Molecular Sequence Data; Nitric Oxide; Oxidants; Oxidation-Reduction; Oxidative Stress; Peptide Hydrolases; Phospholipases A; Phospholipases A2; Spectrometry, Mass, Electrospray Ionization; Spodoptera; Temperature; Time Factors; Tryptophan | 2006 |
Conformational stability of secretory leucocyte protease inhibitor: a protein with no hydrophobic core and very little secondary structure.
Topics: Chromatography, High Pressure Liquid; Cysteine; Cystine; Dithiothreitol; Guanidine; Guanidines; Humans; Hydrophobic and Hydrophilic Interactions; Isothiocyanates; Protein Conformation; Protein Denaturation; Protein Folding; Protein Structure, Secondary; Proteinase Inhibitory Proteins, Secretory; Proteins; Recombinant Proteins; Secretory Leukocyte Peptidase Inhibitor; Thermodynamics; Urea | 2006 |
Redox regulation of MAP kinase phosphatase 3.
Topics: Amides; Catalytic Domain; Cysteine; Dithiothreitol; Dual Specificity Phosphatase 6; Humans; Hydrogen Peroxide; Mass Spectrometry; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Protein Tyrosine Phosphatases; Trypsin | 2006 |
Transcriptional activation of dehalorespiration. Identification of redox-active cysteines regulating dimerization and DNA binding.
Topics: Amino Acid Sequence; Bacterial Proteins; Cysteine; Desulfitobacterium; Diamide; Dimerization; Disulfides; Dithiothreitol; DNA-Binding Proteins; Gene Expression Regulation, Bacterial; Iron-Sulfur Proteins; Molecular Sequence Data; Multigene Family; Mutagenesis, Site-Directed; Oxidation-Reduction; Oxygen; Peptides; Polychlorinated Biphenyls; Protein Structure, Quaternary; Protein Subunits; Sulfhydryl Reagents; Transcription Factors; Transcriptional Activation | 2006 |
A single mutation at Tyr143 of human S-adenosylhomocysteine hydrolase renders the enzyme thermosensitive and affects the oxidation state of bound cofactor nicotinamide-adenine dinucleotide.
Topics: Adenosylhomocysteinase; Amino Acid Substitution; Catalysis; Cysteine; Dithiothreitol; Enzyme Stability; Escherichia coli; Hot Temperature; Humans; Hydrogen Bonding; Hydrolysis; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Mutation; NAD; Oxidation-Reduction; Protein Folding; Protein Structure, Quaternary; Recombinant Proteins; Reducing Agents; Structure-Activity Relationship; Tyrosine | 2006 |
The CXXC motif at the N terminus of an alpha-helical peptide.
Topics: Amino Acid Motifs; Amino Acid Sequence; Circular Dichroism; Computer Simulation; Cysteine; Dithiothreitol; Humans; Hydrogen-Ion Concentration; Oxidation-Reduction; Protein Folding; Protein Structure, Secondary; Thioredoxins | 2006 |
Glutathionylation induces the dissociation of 1-Cys D-peroxiredoxin non-covalent homodimer.
Topics: Binding Sites; Cysteine; Dimerization; Disulfides; Dithiothreitol; Glutathione; Glutathione Transferase; Hydrogen-Ion Concentration; Magnetic Resonance Spectroscopy; Models, Molecular; Oxidation-Reduction; Peroxidases; Peroxiredoxins; Populus; Protein Binding | 2006 |
Role of intrachain disulfides in the activities of the CdtA and CdtC subunits of the cytolethal distending toxin of Actinobacillus actinomycetemcomitans.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Bacterial Toxins; CHO Cells; Cricetinae; Cricetulus; Cysteine; Disulfides; Dithiothreitol; Molecular Sequence Data; Point Mutation; Protein Conformation; Protein Subunits | 2006 |
Thioredoxin-h1 reduces and reactivates the oxidized cytosolic malate dehydrogenase dimer in higher plants.
Topics: Arabidopsis; Chromatography, Affinity; Cysteine; Cytosol; Dimerization; Disulfides; Dithiothreitol; Escherichia coli; Malate Dehydrogenase; Mutation; NADP; Oxidation-Reduction; Peptide Mapping; Recombinant Proteins; Thioredoxins | 2006 |
S-Glutathionylation regulates HDL-associated paraoxonase 1 (PON1) activity.
Topics: Animals; Aryldialkylphosphatase; Cell Line; Cholesterol; Cysteine; Dithiothreitol; Enzyme Activation; Glutathione; Glutathione Disulfide; Lipoproteins, HDL; Macrophages; Mice; Oxidation-Reduction; Reducing Agents | 2006 |
Cys303 in the histidine kinase PhoR is crucial for the phosphotransfer reaction in the PhoPR two-component system in Bacillus subtilis.
Topics: Alanine; Bacillus subtilis; Bacterial Proteins; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Gene Expression Regulation, Bacterial; Histidine Kinase; Mutation; Operon; Phosphorylation; Plasmids; Protein Kinases; Signal Transduction | 2007 |
Entamoeba histolytica: an ecto-phosphatase activity regulated by oxidation-reduction reactions.
Topics: Animals; Chlorides; Cysteine; Dithiothreitol; Entamoeba histolytica; Glutathione; Histidine; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Molybdenum; Nitrophenols; Organophosphorus Compounds; Oxidation-Reduction; Phosphoric Monoester Hydrolases; Sodium Fluoride; Virulence; Zinc Compounds | 2007 |
Thioredoxin-dependent enzymatic activation of mercaptopyruvate sulfurtransferase. An intersubunit disulfide bond serves as a redox switch for activation.
Topics: Amino Acid Sequence; Animals; Binding Sites; Cysteine; Disulfides; Dithiothreitol; Enzyme Activation; Escherichia coli; Molecular Sequence Data; Oxidation-Reduction; Protein Binding; Rats; Sequence Homology, Amino Acid; Sulfurtransferases; Thioredoxins | 2007 |
Direct evidence that two cysteines in the dopamine transporter form a disulfide bond.
Topics: Amino Acid Sequence; Animals; Biological Transport; Cysteine; Disulfides; Dithiothreitol; Dopamine; Dopamine Plasma Membrane Transport Proteins; Drosophila melanogaster; Kinetics; Molecular Sequence Data; Mutant Proteins; Mutation; Structure-Activity Relationship | 2007 |
Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines.
Topics: Acrolein; Animals; Cysteine; Disulfides; Dithiothreitol; Electric Conductivity; Ethyl Methanesulfonate; Humans; Ion Channel Gating; Mice; Mustard Plant; Noxae; Pain; Plant Oils; Transient Receptor Potential Channels | 2007 |
Quantification of protein thiols and dithiols in the picomolar range using sodium borohydride and 4,4'-dithiodipyridine.
Topics: Animals; Borohydrides; Carboxypeptidases; Cathepsin A; Cattle; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Dithiothreitol; Muramidase; Oxidation-Reduction; Proteins; Pyridines; Ribonuclease, Pancreatic; Saccharomyces cerevisiae Proteins; Serum Albumin; Sulfhydryl Compounds; Toluene | 2007 |
Identification of an intersubunit cross-link between substituted cysteine residues located in the putative ATP binding site of the P2X1 receptor.
Topics: Adenosine Triphosphate; Amino Acid Substitution; Animals; Binding Sites; Cysteine; Cystine; Dimerization; Dithiothreitol; Female; Glycosylation; Models, Molecular; Mutagenesis, Site-Directed; Oocytes; Oxidation-Reduction; Patch-Clamp Techniques; Protein Conformation; Protein Folding; Protein Processing, Post-Translational; Protein Subunits; Rats; Receptors, Purinergic P2; Receptors, Purinergic P2X; Recombinant Fusion Proteins; Xenopus laevis | 2007 |
Sodium channel gating modes during redox reaction.
Topics: Action Potentials; Animals; Cysteine; Dithiothreitol; Electric Conductivity; Female; Guinea Pigs; Hydrogen Peroxide; Ion Channel Gating; Male; Myocytes, Cardiac; Oxidation-Reduction; Sodium Channels | 2007 |
Two transmembrane Cys residues are involved in 5-HT4 receptor dimerization.
Topics: Amino Acid Sequence; Animals; Cell Membrane; CHO Cells; Cricetinae; Cricetulus; Cysteine; Dimerization; Disulfides; Dithiothreitol; Humans; Immunoprecipitation; Luminescent Measurements; Point Mutation; Receptors, Adrenergic, beta-2; Receptors, Serotonin, 5-HT4 | 2007 |
Activation of inhibitors by sortase triggers irreversible modification of the active site.
Topics: Alkenes; Aminoacyltransferases; Bacillus anthracis; Bacterial Proteins; Binding Sites; Cysteine; Cysteine Endopeptidases; Dithiothreitol; Drug Design; Enzyme Activation; Enzyme Inhibitors; Inhibitory Concentration 50; Ketones; Kinetics; Models, Biological; Protein Conformation | 2007 |
Reversible inhibition of the fusion activity of measles virus F protein by an engineered intersubunit disulfide bridge.
Topics: Animals; Antiviral Agents; Biotinylation; Chlorocebus aethiops; Cysteine; Disulfides; Dithiothreitol; Drug Design; Measles virus; Membrane Fusion; Molecular Sequence Data; Mutation; Protein Conformation; Protein Engineering; Streptavidin; Vero Cells; Viral Fusion Proteins | 2007 |
Structural insights into the role of the ACTH receptor cysteine residues on receptor function.
Topics: Adrenocorticotropic Hormone; Amino Acid Sequence; Cell Line; Cyclic AMP; Cysteine; Disulfides; Dithiothreitol; Humans; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Receptor, Melanocortin, Type 2; Sulfhydryl Reagents; Transfection | 2007 |
Interaction between lysine 102 and aspartate 338 in the insect amino acid cotransporter KAAT1.
Topics: Amino Acid Sequence; Amino Acid Substitution; Amino Acid Transport Systems, Neutral; Animals; Aspartic Acid; Binding Sites; Biological Transport; Cross-Linking Reagents; Cysteine; Dithiothreitol; Female; Insect Proteins; Kinetics; Lepidoptera; Lysine; Models, Molecular; Molecular Sequence Data; Oocytes; Phenanthrolines; Potassium; Protein Structure, Tertiary; Sequence Homology, Amino Acid; Sodium; Tryptophan; Xenopus laevis | 2007 |
Identification and characterization of 3-substituted pyrazolyl esters as alternate substrates for cathepsin B: the confounding effects of DTT and cysteine in biological assays.
Topics: Biological Assay; Carbon; Cathepsin B; Chemistry, Pharmaceutical; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Drug Design; Esters; Humans; Inhibitory Concentration 50; Models, Chemical; Protein Binding; Reducing Agents; Time Factors | 2007 |
Towards creatine kinase aggregation due to the cysteine modification at the flexible active site and refolding pathway.
Topics: Anilino Naphthalenesulfonates; Animals; Binding Sites; Chromatography, Gel; Circular Dichroism; Creatine Kinase; Cysteine; Dimerization; Dithionitrobenzoic Acid; Dithiothreitol; Dose-Response Relationship, Drug; Fluorescent Dyes; Guanidine; Kinetics; Models, Biological; Muscles; Protein Denaturation; Protein Folding; Protein Renaturation; Protein Structure, Secondary; Protein Structure, Tertiary; Rabbits; Spectrophotometry, Ultraviolet | 2007 |
Oxidative stress-induced expression and modulation of Phosphatase of Regenerating Liver-1 (PRL-1) in mammalian retina.
Topics: Animals; Catalytic Domain; Cell Cycle Proteins; Cell Line; Cells, Cultured; Cycloheximide; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Glutathione; Hydrogen Peroxide; Immediate-Early Proteins; Membrane Proteins; Mice; Oxidants; Oxidation-Reduction; Oxidative Stress; Protein Synthesis Inhibitors; Protein Tyrosine Phosphatases; Rats; Rats, Sprague-Dawley; Recombinant Proteins; Reducing Agents; Retina; Retinal Cone Photoreceptor Cells; Reverse Transcriptase Polymerase Chain Reaction; Swine | 2007 |
Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site.
Topics: Animals; Arginine; Arginine Kinase; Binding Sites; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Enzyme Inhibitors; Grasshoppers; Insect Proteins; Kinetics; Spectrophotometry; Thermodynamics | 2007 |
A possible role for intracellular GSH in spontaneous reaction of a cysteine (T338C) engineered into the Cystic Fibrosis Transmembrane Conductance Regulator.
Topics: Animals; Carmustine; Cysteine; Cystic Fibrosis Transmembrane Conductance Regulator; Dithiothreitol; Electrophysiology; Enzyme Inhibitors; Glutathione; Glutathione Reductase; Mercaptoethanol; Oocytes; Patch-Clamp Techniques; Protein Engineering; Xenopus laevis | 2008 |
Hydrogen sulfide inhibits activity of three isoforms of recombinant nitric oxide synthase.
Topics: Air Pollutants; Biopterins; Cysteine; Dithiothreitol; Enzyme Inhibitors; Humans; Hydrogen Sulfide; NADP; Nitric Oxide Synthase Type I; Nitric Oxide Synthase Type II; Nitric Oxide Synthase Type III; Recombinant Proteins; Sulfates | 2007 |
Structural and functional determinants in the S5-P region of HCN-encoded pacemaker channels revealed by cysteine-scanning substitutions.
Topics: Amino Acid Sequence; Animals; Cadmium Chloride; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Dithiothreitol; Ethyl Methanesulfonate; Female; Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels; Ion Channel Gating; Kinetics; Membrane Potentials; Mice; Microinjections; Models, Molecular; Molecular Sequence Data; Mutation; Oocytes; Permeability; Potassium Channels; Protein Conformation; Protein Structure, Tertiary; Reducing Agents; Structure-Activity Relationship; Sulfhydryl Compounds; Sulfhydryl Reagents; Xenopus laevis | 2008 |
Keap1 modification and nuclear accumulation in response to S-nitrosocysteine.
Topics: Active Transport, Cell Nucleus; Cell Nucleus; Cells, Cultured; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Humans; Intracellular Signaling Peptides and Proteins; Kelch-Like ECH-Associated Protein 1; NF-E2-Related Factor 2; S-Nitrosothiols; Spermine | 2008 |
Is dihydrolipoic acid among the reductive activators of parasite CysHis proteases?
Topics: Animals; Antioxidants; Cathepsin B; Cysteine; Cysteine Endopeptidases; Dithiothreitol; Enzyme Activation; Enzyme Reactivators; Glutathione; Histidine; Iron; Mammals; Oxidation-Reduction; Plasmodium falciparum; Protozoan Proteins; Thioctic Acid; Trypanosoma cruzi | 2008 |
Texture of cooked rice prepared from aged rice and its improvement by reducing agents.
Topics: Cooking; Cysteine; Dithiothreitol; Oryza; Reducing Agents; Sulfites | 2007 |
ALDH-2 deficiency increases cardiovascular oxidative stress--evidence for indirect antioxidative properties.
Topics: Acetaldehyde; Aldehyde Dehydrogenase; Aldehyde Dehydrogenase, Mitochondrial; Animals; Antioxidants; Cardiovascular System; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Doxorubicin; Glutathione; Humans; Mice; Mice, Inbred C57BL; Mitochondria; Models, Biological; Nitroglycerin; Oxidative Stress; Reactive Oxygen Species | 2008 |
Engineered disulfide bonds support the functional rotation mechanism of multidrug efflux pump AcrB.
Topics: 1-Naphthylamine; Amino Acid Substitution; Anti-Bacterial Agents; Cysteine; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Gene Deletion; Microbial Sensitivity Tests; Models, Molecular; Multidrug Resistance-Associated Proteins; Mutagenesis, Site-Directed; Mutant Proteins; Protein Structure, Tertiary; Reducing Agents | 2008 |
Redox modulation of A-type K+ currents in pain-sensing dorsal root ganglion neurons.
Topics: Animals; Chloramines; Cysteine; Dithiothreitol; Ganglia, Spinal; Kv Channel-Interacting Proteins; Kv1.4 Potassium Channel; Neurons, Afferent; Oxidants; Oxidation-Reduction; Pain; Pyridines; Rats; Rats, Wistar; Tosyl Compounds | 2008 |
Two distinct disulfide bonds formed in human heat shock transcription factor 1 act in opposition to regulate its DNA binding activity.
Topics: Binding Sites; Cysteine; Dithiothreitol; DNA; DNA-Binding Proteins; Heat Shock Transcription Factors; Humans; Models, Biological; Oxidation-Reduction; Transcription Factors | 2008 |
An atypical catalytic mechanism involving three cysteines of thioredoxin.
Topics: Amino Acid Sequence; Binding Sites; Catalysis; Cloning, Molecular; Crystallography, X-Ray; Cysteine; Dithiothreitol; Glutaredoxins; Molecular Sequence Data; Mutation; Plant Proteins; Sequence Homology, Amino Acid; Spectrometry, Mass, Electrospray Ionization; Thioredoxin-Disulfide Reductase; Thioredoxins | 2008 |
Differential effects of methionine and cysteine oxidation on [Ca2+] i in cultured hippocampal neurons.
Topics: Animals; Animals, Newborn; Calcium; Calcium Signaling; Cells, Cultured; Cysteine; Dithiothreitol; Hippocampus; Indoles; Inositol 1,4,5-Trisphosphate; Methionine; Neurons; Oxidants; Oxidation-Reduction; Rats; Rats, Sprague-Dawley; Reducing Agents | 2009 |
Interaction with the 5D3 monoclonal antibody is regulated by intramolecular rearrangements but not by covalent dimer formation of the human ABCG2 multidrug transporter.
Topics: Antibodies, Monoclonal; ATP Binding Cassette Transporter, Subfamily G, Member 2; ATP-Binding Cassette Transporters; Cross-Linking Reagents; Cysteine; Dimerization; Dithiothreitol; Epitopes; Formaldehyde; Humans; Membrane Transport Proteins; Models, Biological; Mutation; Neoplasm Proteins; Polymers; Protein Binding; Protein Conformation; Protein Folding | 2008 |
Inactivation of the SMN complex by oxidative stress.
Topics: Amino Acid Sequence; Cross-Linking Reagents; Cyclic AMP Response Element-Binding Protein; Cysteine; Disulfides; Dithiothreitol; HeLa Cells; Humans; Molecular Sequence Data; Naphthoquinones; Nerve Tissue Proteins; Oxidation-Reduction; Oxidative Stress; Reactive Oxygen Species; Ribonucleoproteins, Small Nuclear; RNA-Binding Proteins; Sequence Alignment; Small Molecule Libraries; SMN Complex Proteins | 2008 |
Thermally induced changes in the structure and activity of yeast hexokinase B.
Topics: Amides; Caseins; Chromatography, Gel; Circular Dichroism; Cysteine; Dithiothreitol; Enzyme Stability; gamma-Cyclodextrins; Hexokinase; Hot Temperature; Isoenzymes; Protein Denaturation; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Transition Temperature | 2008 |
An anaerobic bacterial MsrB model reveals catalytic mechanisms, advantages, and disadvantages provided by selenocysteine and cysteine in reduction of methionine-R-sulfoxide.
Topics: Amino Acid Sequence; Bacterial Proteins; Base Sequence; Catalytic Domain; Cell Line; Clostridium; Cysteine; Dithiothreitol; DNA Primers; Humans; Kinetics; Methionine; Methionine Sulfoxide Reductases; Models, Biological; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Oxidation-Reduction; Oxidoreductases; Recombinant Proteins; Selenocysteine; Sequence Homology, Amino Acid; Thioredoxins | 2008 |
Partially irreversible inactivation of mitochondrial aldehyde dehydrogenase by nitroglycerin.
Topics: Acetaldehyde; Aldehyde Dehydrogenase; Aldehyde Dehydrogenase, Mitochondrial; Ascorbic Acid; Cysteine; Dithiothreitol; Drug Tolerance; Enzyme Activation; Glutathione; Humans; Mitochondrial Proteins; NAD; Nitroglycerin; Recombinant Proteins; Vasodilator Agents | 2008 |
Human erythrocyte hemolysis induced by selenium and tellurium compounds increased by GSH or glucose: a possible involvement of reactive oxygen species.
Topics: Adult; Chalcogens; Cysteine; Dithiothreitol; Erythrocytes; Glucose; Glutathione; Hemolysis; Humans; Oxidation-Reduction; Reactive Oxygen Species; Selenium Compounds; Superoxides; Tellurium; Time Factors | 2009 |
Orientation of the monomeric porin OmpG in planar lipid bilayers.
Topics: Bacterial Outer Membrane Proteins; Cyclodextrins; Cysteine; Dithiothreitol; Escherichia coli Proteins; Lipid Bilayers; Porins; Voltage-Dependent Anion Channels | 2008 |
A highly conserved alanine in the S6 domain of the hERG1 K+ channel is required for normal gating.
Topics: Alanine; Amino Acid Sequence; Amino Acid Substitution; Animals; Biophysical Phenomena; Conserved Sequence; Cysteine; Dithiothreitol; Ether-A-Go-Go Potassium Channels; Hydrophobic and Hydrophilic Interactions; Ion Channel Gating; Molecular Sequence Data; Mutant Proteins; Mutation; Oxidation-Reduction; Protein Structure, Secondary; Protein Structure, Tertiary; Structure-Activity Relationship; Xenopus laevis | 2008 |
Location of KCNE1 relative to KCNQ1 in the I(KS) potassium channel by disulfide cross-linking of substituted cysteines.
Topics: Amino Acid Sequence; Animals; CHO Cells; Cricetinae; Cricetulus; Cysteine; Disulfides; Dithiothreitol; Humans; KCNQ1 Potassium Channel; Molecular Sequence Data; Potassium Channels, Voltage-Gated; Protein Structure, Secondary | 2009 |
Prostate specific antigen: one out of five disulfide bridges determines inactivation by reduction.
Topics: Amino Acid Sequence; Cysteine; Disulfides; Dithiothreitol; Humans; Male; Molecular Sequence Data; Oxidation-Reduction; Prostate-Specific Antigen; Protein Conformation; Tissue Kallikreins | 2009 |
Redox regulation of SH2-domain-containing protein tyrosine phosphatases by two backdoor cysteines.
Topics: Biocatalysis; Catalytic Domain; Cysteine; Disulfides; Dithiothreitol; Enzyme Activation; Glutathione; Humans; Hydrogen Peroxide; Kinetics; Mutagenesis; Mutant Proteins; Oxidation-Reduction; SH2 Domain-Containing Protein Tyrosine Phosphatases; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Structure-Activity Relationship; Thioredoxin-Disulfide Reductase; Thioredoxins; Time Factors | 2009 |
Domain interplay mediated by an essential disulfide linkage is critical for the activity and secretion of the metastasis-promoting enzyme autotaxin.
Topics: Amino Acid Sequence; Animals; Biocatalysis; Cell Line; Cysteine; Disulfides; Dithiothreitol; Endopeptidases; Enzyme Activation; Humans; Lysine; Molecular Sequence Data; Neoplasm Metastasis; Phosphoric Diester Hydrolases; Protein Structure, Tertiary; Pyrophosphatases; Rats; Structure-Activity Relationship | 2009 |
Modulation of Cx46 hemichannels by nitric oxide.
Topics: Animals; Cataract; Connexin 43; Connexins; Cysteine; Dithiothreitol; Fluorescent Dyes; Gap Junctions; Ion Channel Gating; Kinetics; Membrane Potentials; Mutation; Nitric Oxide; Nitric Oxide Donors; Oocytes; Oxidation-Reduction; Permeability; Rats; Recombinant Fusion Proteins; Reducing Agents; S-Nitrosoglutathione; Xenopus laevis | 2009 |
State-dependent accessibility of the P-S6 linker of pacemaker (HCN) channels supports a dynamic pore-to-gate coupling model.
Topics: Animals; Blotting, Western; Cadmium; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Dithiothreitol; Electrophysiology; Mice; Models, Biological; Oocytes; Structure-Activity Relationship; Xenopus | 2009 |
Investigation of peptide reactivity of pro-hapten skin sensitizers using a peroxidase-peroxide oxidation system.
Topics: Catechols; Chromatography, High Pressure Liquid; Cysteine; Dithiothreitol; Haptens; Oxidation-Reduction; Peptides; Peroxidases; Peroxides; Skin; Tandem Mass Spectrometry | 2009 |
Small-molecule reductants inhibit multicatalytic activity of AA-NADase from Agkistrodon acutus venom by reducing the disulfide-bonds and Cu(II) of enzyme.
Topics: Animals; Copper; Crotalid Venoms; Cysteine; Disulfides; Dithiothreitol; Glutathione; NAD+ Nucleosidase; Oxidation-Reduction; Phosphines | 2010 |
Inward-facing conformation of glutamate transporters as revealed by their inverted-topology structural repeats.
Topics: Amino Acid Transport System X-AG; Animals; Archaeal Proteins; Biological Transport; Cross-Linking Reagents; Cysteine; Cytoplasm; Dithiothreitol; Models, Molecular; Mutant Proteins; Phenanthrolines; Protein Structure, Secondary; Protein Structure, Tertiary; Pyrococcus horikoshii; Rabbits; Repetitive Sequences, Amino Acid; Substrate Specificity; Xenopus | 2009 |
Pathogenic cysteine mutations affect progranulin function and production of mature granulins.
Topics: Animals; Arginine; Axons; Brain; Cell Enlargement; Cell Line, Transformed; Cysteine; Dithiothreitol; Embryo, Mammalian; Enzyme-Linked Immunosorbent Assay; Family Health; Frontotemporal Dementia; Gene Expression Regulation; Genetic Predisposition to Disease; Green Fluorescent Proteins; Humans; Intercellular Signaling Peptides and Proteins; Motor Neurons; Mutation; Neurites; Pancreatic Elastase; Progranulins; Rats; Rats, Wistar; RNA, Messenger; Transfection; Tyrosine | 2010 |
Role of a disulphide bond in Helicobacter pylori arginase.
Topics: Arginase; Catalysis; Cobalt; Cysteine; Dithiothreitol; DNA Mutational Analysis; Enzyme Stability; Helicobacter pylori; Manganese; Mutation; Protein Denaturation | 2010 |
Activation of rat liver microsomal glutathione transferase by hepsin.
Topics: Animals; Benzamidines; Cysteine; Dimerization; Disulfides; Dithiothreitol; Enzyme Activation; Glutathione Transferase; Hydrolysis; Male; Microsomes, Liver; Protein Structure, Tertiary; Rats; Rats, Sprague-Dawley; Serine Endopeptidases; Serine Proteinase Inhibitors | 2010 |
S-Nitrosylation of DRP1 does not affect enzymatic activity and is not specific to Alzheimer's disease.
Topics: Aging; Alzheimer Disease; Biotin; Brain; Cell Line, Transformed; Cysteine; Dithiothreitol; Dynamins; GTP Phosphohydrolases; Humans; Microscopy, Electron, Transmission; Microtubule-Associated Proteins; Mitochondrial Diseases; Mitochondrial Proteins; Postmortem Changes; Protein Multimerization | 2010 |
Long-lasting inhibition of presynaptic metabolism and neurotransmitter release by protein S-nitrosylation.
Topics: Animals; Cysteine; Dithiothreitol; Down-Regulation; Glyceraldehyde-3-Phosphate Dehydrogenase (NADP+)(Phosphorylating); Male; Neurotransmitter Agents; Presynaptic Terminals; Protein Processing, Post-Translational; Pyruvic Acid; Rats; Rats, Sprague-Dawley; S-Nitrosothiols; Synaptic Transmission; Time Factors | 2010 |
Cysteines in the loop between IS5 and the pore helix of Ca(V)3.1 are essential for channel gating.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Calcium Channels, T-Type; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; HEK293 Cells; Humans; Ion Channel Gating; Mice; Models, Molecular; Molecular Sequence Data; Patch-Clamp Techniques; Protein Structure, Tertiary | 2010 |
Nitric oxide-mediated inhibition of taurocholate uptake involves S-nitrosylation of NTCP.
Topics: Cell Line, Tumor; Cell Membrane; Cysteine; Dithiothreitol; Humans; Nitric Oxide; Organic Anion Transporters, Sodium-Dependent; Phosphorylation; S-Nitrosothiols; Symporters; Taurocholic Acid | 2011 |
Redox state of troponin C cysteine in the D/E helix alters the C-domain affinity for the thin filament of vertebrate striated muscle.
Topics: Animals; Chickens; Cysteine; Dithiothreitol; Muscle, Striated; Oxidation-Reduction; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Rabbits; Rats; Recombinant Proteins; Troponin C; Troponin I; Troponin T; Vertebrates | 2011 |
Improvement of translation efficiency in an Escherichia coli cell-free protein system using cysteine.
Topics: Cell Fractionation; Cell-Free System; Chromatography, High Pressure Liquid; Cysteine; Cystine; Disulfides; Dithiothreitol; Escherichia coli; Glutathione; Glutathione Disulfide; Glutathione Transferase; Green Fluorescent Proteins; Protein Biosynthesis; RNA, Messenger; Subcellular Fractions | 2011 |
Novel inhibitors of glyceraldehyde-3-phosphate dehydrogenase: covalent modification of NAD-binding site by aromatic thiols.
Topics: Animals; Catalytic Domain; Cysteine; Dithiothreitol; Enzyme Assays; Enzyme Inhibitors; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating); Models, Molecular; Rabbits; Sulfhydryl Compounds; Thermodynamics; Titrimetry | 2010 |
Cysteine ubiquitination of PTS1 receptor Pex5p regulates Pex5p recycling.
Topics: Amino Acid Motifs; Animals; Cells, Cultured; CHO Cells; Cricetinae; Cricetulus; Cysteine; Cytosol; Dithiothreitol; HeLa Cells; Humans; Membrane Proteins; Mutation; Peroxisomal Targeting Signal 2 Receptor; Peroxisome-Targeting Signal 1 Receptor; Peroxisomes; Protein Binding; Protein Structure, Tertiary; Protein Transport; Rats; Receptors, Cytoplasmic and Nuclear; Repressor Proteins; Tumor Cells, Cultured; Ubiquitination | 2011 |
Reducing conditions are the key for efficient production of active ribonuclease inhibitor in Escherichia coli.
Topics: Amino Acid Motifs; Cysteine; Dithiothreitol; Escherichia coli; Leucine-Rich Repeat Proteins; Periplasm; Proteins; Recombinant Proteins; Reducing Agents | 2011 |
Structural analysis of alpha-helical proteins from wool using cysteine labelling and mass spectrometry.
Topics: Amino Acid Sequence; Animals; Cysteine; Dithiothreitol; Indicators and Reagents; Keratins; Mass Spectrometry; Molecular Sequence Data; Protein Structure, Secondary; Staining and Labeling; Wool | 2011 |
Topoisomerase I inactivation by a novel thiol reactive naphthoquinone.
Topics: Cysteine; Dithiothreitol; DNA Topoisomerases, Type I; HeLa Cells; Humans; Naphthoquinones; Reducing Agents; Topoisomerase I Inhibitors | 2011 |
Plastidic phosphoglycerate kinase from Phaeodactylum tricornutum: on the critical role of cysteine residues for the enzyme function.
Topics: Amino Acid Sequence; Cloning, Molecular; Cysteine; Diamide; Diatoms; Disulfides; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Enzyme Assays; Enzyme Inhibitors; Escherichia coli; Glutathione; Hydrogen Peroxide; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Nitroprusside; Oxidation-Reduction; Phosphoglycerate Kinase; Plasmids; Plastids; Recombinant Proteins; Sequence Alignment; Sulfenic Acids; Thioredoxins | 2012 |
Hydrogen peroxide targets the cysteine at the active site and irreversibly inactivates creatine kinase.
Topics: Acetylcysteine; Anilino Naphthalenesulfonates; Binding Sites; Brain; Catalytic Domain; Creatine Kinase, BB Form; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Glutathione; Humans; Hydrogen Peroxide; Hydrogen-Ion Concentration; Kinetics; Models, Molecular; Protein Binding; Reactive Oxygen Species; Spectrometry, Fluorescence; Structure-Activity Relationship; Sulfhydryl Compounds | 2011 |
Fukutin-related protein resides in the Golgi cisternae of skeletal muscle fibres and forms disulfide-linked homodimers via an N-terminal interaction.
Topics: Animals; Biomarkers; Cell Line; Cell Survival; Cysteine; Disulfides; Dithiothreitol; Glycosylation; Golgi Apparatus; Humans; Immunoprecipitation; Mannose; Models, Biological; Multiprotein Complexes; Muscle Fibers, Skeletal; Myofibrils; Oligosaccharides; Oxidation-Reduction; Pentosyltransferases; Protein Binding; Protein Multimerization; Protein Transport; Proteins; Receptors, Fibroblast Growth Factor; Recombinant Proteins; Sialoglycoproteins; Two-Hybrid System Techniques | 2011 |
Branched intermediate formation is the slowest step in the protein splicing reaction of the Ala1 KlbA intein from Methanococcus jannaschii.
Topics: Amino Acid Sequence; Archaeal Proteins; Base Sequence; Cysteine; Dithiothreitol; Exteins; Fluorescent Dyes; Hydrogen-Ion Concentration; Inteins; Kinetics; Methanococcus; Molecular Sequence Data; Peptides; Protein Splicing; Structure-Activity Relationship; Tyrosine | 2011 |
GluN1-specific redox effects on the kinetic mechanism of NMDA receptor activation.
Topics: Animals; Cysteine; Disulfides; Dithiothreitol; Ion Channel Gating; Kinetics; Ligands; Oxidation-Reduction; Protein Structure, Tertiary; Protein Subunits; Rats; Receptors, N-Methyl-D-Aspartate; Time Factors; Xenopus | 2011 |
Reactivation of a thermostable lipase by solid phase unfolding/refolding effect of cysteine residues on refolding efficiency.
Topics: Bacterial Proteins; Biotechnology; Cysteine; Dithiothreitol; Enzyme Activation; Enzyme Stability; Enzymes, Immobilized; Geobacillus; Glyoxylates; Lipase; Models, Molecular; Oxidation-Reduction; Protein Refolding; Sepharose | 2011 |
Influence of 2,5-dichloro-1,4-benzoquinone on jack bean urease activity. Inhibitory effect, total reducing capacity and DPPH radical scavenging activity.
Topics: Benzoquinones; Biphenyl Compounds; Canavalia; Cysteine; Dithiothreitol; Enzyme Activation; Enzyme Inhibitors; Enzyme Reactivators; Free Radical Scavengers; Glutathione; Oxidation-Reduction; Photolysis; Picrates; Solutions; Spectrophotometry; Urease | 2011 |
Identification of a redox-sensitive switch within the JAK2 catalytic domain.
Topics: Animals; Catalytic Domain; Cell Line; Cysteine; Diabetes Mellitus; Disease Models, Animal; Dithiothreitol; Enzyme Activation; Hydrogen Peroxide; Insulin-Secreting Cells; Janus Kinase 2; Mice; Mutagenesis, Site-Directed; Mutation; Oxidation-Reduction; Oxidative Stress; Phosphorylation; STAT5 Transcription Factor | 2012 |
Possible involvement of S-nitrosylation of brain cyclooxygenase-1 in bombesin-induced central activation of adrenomedullary outflow in rats.
Topics: 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; Adrenal Medulla; Animals; Benzoates; Bombesin; Brain; Catecholamines; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Ethylmaleimide; Imidazoles; Injections, Intraventricular; Male; Molsidomine; Nitric Oxide Donors; Oxadiazoles; Paraventricular Hypothalamic Nucleus; Quinoxalines; Rats; Rats, Wistar; S-Nitrosothiols; Sulfhydryl Reagents | 2012 |
Essential cysteine residues for human RNase κ catalytic activity.
Topics: Animals; Catalysis; Cysteine; Disulfides; Dithiothreitol; Endoribonucleases; Humans; Pichia; Recombinant Proteins | 2012 |
Insight into the redox regulation of the phosphoglucan phosphatase SEX4 involved in starch degradation.
Topics: Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Catalytic Domain; Chromatography, Liquid; Cysteine; Disulfides; Dithiothreitol; Dual-Specificity Phosphatases; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Glucans; Hydrogen Peroxide; Kinetics; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Mutation; Nitrophenols; Organophosphorus Compounds; Oxidants; Oxidation-Reduction; Phosphorylation; Starch | 2013 |
Leishmania amazonensis: inhibition of 3'-nucleotidase activity by Cu2+ ions.
Topics: Animals; Ascorbic Acid; Copper; Cricetinae; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Glutathione; Humans; Hydrogen Peroxide; Hydrogen-Ion Concentration; Leishmania mexicana; Mercaptoethanol; Nucleotidases; Oxidation-Reduction; Oxygen; Phenanthrolines; Reactive Oxygen Species | 2012 |
Purification and characterization of ZmRIP1, a novel reductant-inhibited protein tyrosine phosphatase from maize.
Topics: Amino Acid Sequence; Arabidopsis; Arsenicals; Cell Nucleus; Chloroplasts; Cloning, Molecular; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Activation; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Glutathione Transferase; Molecular Sequence Data; Oxidation-Reduction; Plant Proteins; Plants, Genetically Modified; Point Mutation; Protein Transport; Protein Tyrosine Phosphatases; Protoplasts; Signal Transduction; Zea mays | 2012 |
Nitric oxide decreases activity and levels of the 11S proteasome activator PA28 in the vasculature.
Topics: Analysis of Variance; Animals; Aorta; Carotid Arteries; Carotid Artery Injuries; Caspases; Cells, Cultured; Chymotrypsin; Cysteine; Dithiothreitol; Mercuric Chloride; Myocytes, Smooth Muscle; Nitric Oxide; Proteasome Endopeptidase Complex; Rats; Rats, Sprague-Dawley; Reducing Agents; S-Nitrosothiols; Trypsin | 2012 |
Thiol-reactive compounds from garlic inhibit the epithelial sodium channel (ENaC).
Topics: Animals; Cysteine; Disulfides; Dithiothreitol; Epithelial Sodium Channel Blockers; Epithelial Sodium Channels; Garlic; Humans; Membrane Potentials; Microelectrodes; Oocytes; Patch-Clamp Techniques; Plant Exudates; Sulfhydryl Compounds; Sulfinic Acids; Xenopus laevis | 2012 |
S-glutathionylation regulates GTP-binding of Rac2.
Topics: Animals; Cysteine; Dithiothreitol; Enzyme Activation; Glutathione; Guanosine Triphosphate; NADP; rac GTP-Binding Proteins; RAC2 GTP-Binding Protein; Recombinant Proteins | 2012 |
Functional consequences of sulfhydryl modification of the γ-aminobutyric acid transporter 1 at a single solvent-exposed cysteine residue.
Topics: Animals; Cysteine; Dithiothreitol; Ethylmaleimide; Female; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Gene Expression; Humans; Kinetics; Mesylates; Mutation; Oocytes; Patch-Clamp Techniques; Rhodamines; Sodium Chloride; Sulfhydryl Reagents; Transfection; Valproic Acid; Xenopus laevis | 2012 |
Control of STN7 transcript abundance and transient STN7 dimerisation are involved in the regulation of STN7 activity.
Topics: Arabidopsis; Arabidopsis Proteins; Cysteine; Cytochrome b6f Complex; Disulfides; Dithiothreitol; Diuron; Enzyme Activation; Light; Light-Harvesting Protein Complexes; Oxidation-Reduction; Phosphorylation; Photosystem II Protein Complex; Protein Multimerization; Protein Processing, Post-Translational; Protein Serine-Threonine Kinases; RNA, Messenger; Serine; Time Factors | 2013 |
Reversible inactivation of dihydrolipoamide dehydrogenase by mitochondrial hydrogen peroxide.
Topics: Animals; Antimycin A; Brain; Catalase; Cysteine; Dihydrolipoamide Dehydrogenase; Dithiothreitol; Electron Transport Complex I; Electron Transport Complex III; Ethylmaleimide; Glutathione; Hydrogen Peroxide; Malates; Male; Mitochondria; Oxidation-Reduction; Oxidative Stress; Pyruvic Acid; Rats; Rats, Sprague-Dawley; Rotenone; Succinic Acid; Superoxides | 2013 |
Unique requirements for mono- and polyubiquitination of the peroxisomal targeting signal co-receptor, Pex20.
Topics: Binding Sites; Cysteine; Dithiothreitol; Fungal Proteins; Immunoblotting; Lysine; Microscopy, Fluorescence; Models, Biological; Mutation; Peroxisomal Targeting Signal 2 Receptor; Peroxisomes; Pichia; Polyubiquitin; Promoter Regions, Genetic; Receptors, Cytoplasmic and Nuclear; RING Finger Domains; Ubiquitin-Conjugating Enzymes; Ubiquitin-Protein Ligases; Ubiquitination | 2013 |
Reversible inactivation of deubiquitinases by reactive oxygen species in vitro and in cells.
Topics: Animals; Biocatalysis; Cysteine; Dithiothreitol; Endopeptidases; Enzyme Activation; HEK293 Cells; Hep G2 Cells; Humans; Hydrogen Peroxide; Kinetics; Mice; Models, Biological; Oxidation-Reduction; Oxidative Stress; Proliferating Cell Nuclear Antigen; Reactive Oxygen Species; Ubiquitination | 2013 |
Redox state of p63 and p73 core domains regulates sequence-specific DNA binding.
Topics: Base Sequence; Cysteine; Diamide; Dithiothreitol; DNA; DNA-Binding Proteins; Edetic Acid; Electrophoresis, Agar Gel; Electrophoretic Mobility Shift Assay; Humans; Nuclear Proteins; Oxidation-Reduction; Protein Interaction Mapping; Protein Structure, Tertiary; Tumor Protein p73; Tumor Suppressor Protein p53; Tumor Suppressor Proteins; Zinc | 2013 |
Importance of cysteine residues in the thyroid hormone transporter MCT8.
Topics: 4-Chloromercuribenzenesulfonate; Alanine; Amino Acid Substitution; Cell Line, Tumor; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Humans; Monocarboxylic Acid Transporters; Mutagenesis, Site-Directed; Point Mutation; Sulfhydryl Reagents; Symporters; Time Factors; Transfection | 2013 |
Cysteine-modulated colorimetric sensing of extracellular Mg2+ in rat brain based on the strong chelation interaction between dithiothreitol and Mg2+.
Topics: Animals; Cerebral Cortex; Chelating Agents; Colorimetry; Cysteine; Dithiothreitol; Magnesium; Male; Rats; Rats, Sprague-Dawley; Surface Properties | 2013 |
Intermolecular disulfide bonds between nucleoporins regulate karyopherin-dependent nuclear transport.
Topics: Active Transport, Cell Nucleus; beta Karyopherins; Cell Membrane Permeability; Cysteine; Dithiothreitol; Gene Knockdown Techniques; HeLa Cells; Humans; Hydrogen Peroxide; Mutagenesis, Site-Directed; Mutation; Nuclear Pore; Nuclear Pore Complex Proteins; Oxidation-Reduction; Reactive Oxygen Species | 2013 |
Enzymatic activity and catalytic hydrogen evolution in reduced and oxidized urease at mercury surfaces.
Topics: Adsorption; Catalysis; Cysteine; Disulfides; Dithiothreitol; Electrochemical Techniques; Electrodes; Mercury; Oxidation-Reduction; Protein Denaturation; Protein Folding; Sulfhydryl Compounds; Surface Properties; Temperature; Urease | 2013 |
Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids.
Topics: Ascorbic Acid; Cyclohexanones; Cysteine; Dithiothreitol; Ethylmaleimide; Iodoacetamide; Methyl Methanesulfonate; Oxidation-Reduction; Proteins; Reactive Oxygen Species; Spectrometry, Mass, Electrospray Ionization; Sulfenic Acids; Sulfhydryl Compounds | 2013 |
Evaluating the conformation and binding interface of cap-binding proteins and complexes via ultraviolet photodissociation mass spectrometry.
Topics: Amino Acid Sequence; Binding Sites; Cysteine; Dithiothreitol; Eukaryotic Initiation Factor-4E; Eukaryotic Initiation Factor-4F; Eukaryotic Initiation Factor-4G; Guanosine Triphosphate; Lysine; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Plant Proteins; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Alignment; Triticum | 2013 |
The membrane proximal region of the cannabinoid receptor CB1 N-terminus can allosterically modulate ligand affinity.
Topics: Allosteric Regulation; Amino Acid Sequence; Animals; Binding Sites; Chlorocebus aethiops; COS Cells; Cyclohexanols; Cysteine; Disulfides; Dithiothreitol; Humans; Indoles; Ligands; Phenylurea Compounds; Piperidines; Pyridines; Rats; Receptor, Cannabinoid, CB1 | 2013 |
Functionalized gold nanoparticles for the detection of arsenic in water.
Topics: Arsenic; Cysteine; Dithiothreitol; Glutathione; Gold; Limit of Detection; Metal Nanoparticles; Water Pollutants, Chemical | 2014 |
Cysteine mutagenesis reveals alternate proximity between transmembrane domain 2 and hairpin loop 1 of the glutamate transporter EAAT1.
Topics: Amino Acid Sequence; Aspartic Acid; Cadmium Chloride; Cross-Linking Reagents; Cysteine; Dithiothreitol; Excitatory Amino Acid Transporter 1; Glutamic Acid; HeLa Cells; Humans; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Phenanthrolines; Protein Structure, Tertiary; Structure-Activity Relationship | 2014 |
The autolysis of human HtrA1 is governed by the redox state of its N-terminal domain.
Topics: Biocatalysis; Cysteine; Cystine; Databases, Protein; Dithiothreitol; Enzyme Stability; Glutathione; High-Temperature Requirement A Serine Peptidase 1; Humans; Models, Molecular; Osmolar Concentration; Oxidation-Reduction; Oxidative Stress; Peptide Fragments; Protein Structure, Tertiary; Protein Unfolding; Proteolysis; Recombinant Proteins; Reducing Agents; Serine Endopeptidases; Thioredoxins | 2014 |
Purification and characterization of an extracellular, thermo-alkali-stable, metal tolerant laccase from Bacillus tequilensis SN4.
Topics: Analysis of Variance; Bacillus; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Enzyme Stability; Hydrogen-Ion Concentration; Kinetics; Laccase; Mercaptoethanol; Oxidoreductases; Pyrogallol; Sodium Azide; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Substrate Specificity; Temperature | 2014 |
Cysteines introduced into extracellular loops 1 and 4 of human P-glycoprotein that are close only in the open conformation spontaneously form a disulfide bond that inhibits drug efflux and ATPase activity.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Animals; ATP Binding Cassette Transporter, Subfamily B; Binding Sites; Cell Line; Cysteine; Disulfides; Dithiothreitol; Drug Resistance, Multiple; Glycoside Hydrolases; HEK293 Cells; Humans; Hydrolysis; Immunoblotting; Models, Molecular; Mutation; Pharmaceutical Preparations; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary | 2014 |
Thioredoxin-dependent redox regulation of chloroplastic phosphoglycerate kinase from Chlamydomonas reinhardtii.
Topics: Animals; Chlamydomonas reinhardtii; Chloroplast Thioredoxins; Chloroplasts; Conserved Sequence; Cysteine; Disulfides; Dithiothreitol; Humans; Hydrogen-Ion Concentration; Kinetics; Light; Models, Molecular; Mutagenesis, Site-Directed; Mutant Proteins; Oxidation-Reduction; Peptide Mapping; Phosphoglycerate Kinase; Protein Structure, Tertiary; Sequence Analysis, Protein; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sus scrofa | 2014 |
Investigating the photostability of quantum dots at the single-molecule level.
Topics: Cysteine; Dithiothreitol; Gallic Acid; Glutathione; Photobleaching; Quantum Dots; Thioglycolates; Thiourea | 2014 |
Nitroxyl (HNO) reacts with molecular oxygen and forms peroxynitrite at physiological pH. Biological Implications.
Topics: Acetylcysteine; Algorithms; Animals; Boron Compounds; Boronic Acids; Captopril; Cattle; Chromatography, High Pressure Liquid; Coumarins; Cysteine; Dithiothreitol; Humans; Hydrogen Sulfide; Hydrogen-Ion Concentration; Kinetics; Mass Spectrometry; Models, Chemical; Nitrogen Oxides; Oxidation-Reduction; Oxygen; Peroxynitrous Acid; Serum Albumin; Spectrophotometry; Sulfhydryl Compounds | 2014 |
A sensitive and selective detection method for thiol compounds using novel fluorescence probe.
Topics: Cysteine; Dithiothreitol; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Glutathione; Limit of Detection; Sulfhydryl Compounds | 2014 |
Peroxide-mediated oxidation and inhibition of the peptidyl-prolyl isomerase Pin1.
Topics: Biocatalysis; Catalytic Domain; Crystallography, X-Ray; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Humans; Hydrogen Peroxide; Kinetics; Models, Molecular; NIMA-Interacting Peptidylprolyl Isomerase; Oxidants; Oxidation-Reduction; Peptidylprolyl Isomerase; Protein Structure, Tertiary; Spectrometry, Mass, Electrospray Ionization; Sulfenic Acids; Sulfinic Acids; Time Factors | 2015 |
Identification of reduction-susceptible disulfide bonds in transferrin by differential alkylation using O(16)/O(18) labeled iodoacetic acid.
Topics: Alkylation; Chromatography, High Pressure Liquid; Cysteine; Cystine; Dithiothreitol; Feasibility Studies; Humans; Indicators and Reagents; Iodoacetic Acid; Models, Molecular; Oxidation-Reduction; Oxygen Isotopes; Protein Conformation; Protein Stability; Reducing Agents; Tandem Mass Spectrometry; Transferrin | 2015 |
The C-terminal peptide plays a role in the formation of an intermediate form during the transition between xanthine dehydrogenase and xanthine oxidase.
Topics: Amino Acid Sequence; Amino Acid Substitution; Animals; Crystallography, X-Ray; Cysteine; Dithiothreitol; Guanidine; Liver; Models, Biological; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; NAD; Protein Conformation; Rats; Recombinant Proteins; Sequence Deletion; Sequence Homology, Amino Acid; Static Electricity; Sulfhydryl Reagents; Xanthine Dehydrogenase; Xanthine Oxidase | 2015 |
Eukaryotic formylglycine-generating enzyme catalyses a monooxygenase type of reaction.
Topics: Alanine; Animals; Baculoviridae; Biocatalysis; Catalytic Domain; Cysteine; Disulfides; Dithiothreitol; Enzyme Assays; Gene Expression; Glycine; Humans; Kinetics; Mixed Function Oxygenases; Oxidation-Reduction; Oxidoreductases Acting on Sulfur Group Donors; Oxygen; Recombinant Proteins; Sf9 Cells; Spodoptera; Sulfatases | 2015 |
Cysteine as a Monothiol Reducing Agent to Prevent Copper-Mediated Oxidation of Interferon Beta During PEGylation by CuAAC.
Topics: Amino Acid Substitution; Catalysis; Copper; Cycloaddition Reaction; Cysteine; Dithiothreitol; Interferon beta-1b; Oxidation-Reduction; Polyethylene Glycols; Reducing Agents | 2015 |
Dithiothreitol (DTT) Acts as a Specific, UV-inducible Cross-linker in Elucidation of Protein-RNA Interactions.
Topics: Cross-Linking Reagents; Cysteine; Dithiothreitol; Mass Spectrometry; Proteins; RNA; Uracil | 2015 |
Evidence for redox sensing by a human cardiac calcium channel.
Topics: Calcium Channels, L-Type; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Humans; Mutation; Myocardium; Oxidants; Oxidation-Reduction; Protein Folding; Protein Isoforms; Sulfhydryl Compounds | 2016 |
Dependence of the structure and mechanics of metaphase chromosomes on oxidized cysteines.
Topics: Adenosine Triphosphatases; Animals; Cell Cycle Proteins; Cell Line; Chromosomal Proteins, Non-Histone; Chromosomes, Human; Cohesins; Cysteine; Dithiothreitol; DNA-Binding Proteins; Electrophoresis, Gel, Two-Dimensional; Guanine Nucleotide Exchange Factors; HEK293 Cells; Humans; Karyotype; Mass Spectrometry; Metaphase; Micromanipulation; Multiprotein Complexes; Notophthalmus viridescens; Nuclear Matrix-Associated Proteins; Nuclear Proteins; Octamer Transcription Factors; Oxidation-Reduction; Phosphines; RNA-Binding Proteins | 2016 |
Identification of a Disulfide Bridge in Sodium-Coupled Neutral Amino Acid Transporter 2(SNAT2) by Chemical Modification.
Topics: Alanine; Amino Acid Transport System A; Amino Acid Transport Systems; Animals; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; HEK293 Cells; Humans; Ion Transport; Mutagenesis; Mutation; Polyethylene Glycols; Rats; Sodium; Sulfhydryl Compounds | 2016 |
Redox regulation of ATP sulfurylase in microalgae.
Topics: Amino Acid Sequence; Chromatography, Liquid; Cysteine; Dithiothreitol; Microalgae; Models, Molecular; Oxidation-Reduction; Peptides; Sequence Alignment; Sulfate Adenylyltransferase; Tandem Mass Spectrometry | 2016 |
Secretagogin Is a Redox-Responsive Ca
Topics: Animals; Binding Sites; Calcium; Cell Line, Tumor; Cell Nucleus; Cloning, Molecular; Cysteine; Cytosol; Dithiothreitol; Endoplasmic Reticulum; Escherichia coli; Gene Expression Regulation; Insulin-Secreting Cells; Mice; Models, Molecular; Oxidation-Reduction; Protein Binding; Protein Domains; Protein Multimerization; Protein Structure, Secondary; Recombinant Proteins; Secretagogins | 2017 |
Modification of Cysteine.
Topics: Alkylation; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Formates; Indicators and Reagents; Iodoacetamide; Iodoacetic Acid; Oxidation-Reduction; Peptides; Proteins; Pyridines | 2017 |
Epiberberine, a natural protoberberine alkaloid, inhibits urease of Helicobacter pylori and jack bean: Susceptibility and mechanism.
Topics: Berberine; Canavalia; Coptis chinensis; Cysteine; Dithiothreitol; Drugs, Chinese Herbal; Glutathione; Helicobacter pylori; Hydroxamic Acids; Kinetics; Molecular Docking Simulation; Molecular Structure; Plant Proteins; Urease | 2017 |
Redox status of serum apolipoprotein E and its impact on HDL cholesterol levels.
Topics: Apolipoprotein A-II; Apolipoprotein E2; Apolipoprotein E3; Apolipoprotein E4; Apolipoproteins E; Cholesterol, HDL; Cysteine; Diamide; Dimerization; Dithiothreitol; Electrophoretic Mobility Shift Assay; HEK293 Cells; Humans; Indicators and Reagents; Molecular Weight; Oxidation-Reduction; Photochemical Processes; Polyethylene Glycols; Solubility; Sulfhydryl Reagents; Ultraviolet Rays | 2017 |
Selective detection of ZnO nanoparticles in aqueous suspension by capillary electrophoresis analysis using dithiothreitol and L-cysteine adsorbates.
Topics: Cysteine; Dithiothreitol; Electrophoresis, Capillary; Nanoparticles; Suspensions; Water; Zinc Oxide | 2017 |
Systematic Evaluation of Protein Reduction and Alkylation Reveals Massive Unspecific Side Effects by Iodine-containing Reagents.
Topics: Alkylation; Cysteine; Dithiothreitol; HeLa Cells; Humans; Indicators and Reagents; Iodine; Mercaptoethanol; Methionine; Proteins; Proteomics; Tandem Mass Spectrometry | 2017 |
In Vitro Alkylation Methods for Assessing the Protein Redox State.
Topics: Alkylation; Chloroplasts; Cysteine; Dithiothreitol; Electrophoresis, Polyacrylamide Gel; Gene Expression; Glutaredoxins; Glutathione Disulfide; Hydrogen Peroxide; Kinetics; Maleimides; Oxidation-Reduction; Plant Proteins; Protein Processing, Post-Translational; Recombinant Proteins; Thioredoxins | 2017 |
Cysteine perthiosulfenic acid (Cys-SSOH): A novel intermediate in thiol-based redox signaling?
Topics: Cyclohexanones; Cysteine; Dithiothreitol; HEK293 Cells; Humans; Hydrogen Peroxide; Models, Molecular; NADPH Oxidases; Oxidation-Reduction; Protein-Tyrosine Kinases; Proteins; Signal Transduction; Sulfenic Acids; Sulfhydryl Compounds | 2018 |
Full and partial agonists evoke distinct structural changes in opening the muscle acetylcholine receptor channel.
Topics: Amino Acid Substitution; Cell Line; Cross-Linking Reagents; Cysteine; Dithiothreitol; Humans; Hydrogen Peroxide; Ion Channel Gating; Receptors, Cholinergic; Sulfhydryl Reagents | 2018 |
NADP
Topics: Animals; Cadmium; Calorimetry; Chelating Agents; Crystallography, X-Ray; Cysteine; Cytosol; Dithiothreitol; Enzyme Activation; Glutathione; Isocitrate Dehydrogenase; Mice; NADP; Protein Conformation; Spectrophotometry, Ultraviolet | 2018 |
Paired-Cysteine Scanning Reveals Conformationally Sensitive Proximity between the TM4b-4c Loop and TM8 of the Glutamate Transporter EAAT1.
Topics: Cysteine; Dithiothreitol; Excitatory Amino Acid Transporter 1; HeLa Cells; Humans; Molecular Conformation; Mutagenesis; Protein Synthesis Inhibitors; Structure-Activity Relationship; Sulfhydryl Compounds; Tetraspanins | 2019 |
Membrane Chaperoning of a Thylakoid Protease Whose Structural Stability Is Modified by the Protonmotive Force.
Topics: Arabidopsis; Arabidopsis Proteins; Circadian Rhythm; Cysteine; Disulfides; Dithiothreitol; Enzyme Stability; Escherichia coli; Genes, Suppressor; Intracellular Membranes; Lipid Bilayers; Models, Biological; Molecular Chaperones; Mutation; Oxidation-Reduction; Protein Conformation; Proton-Motive Force; Serine Endopeptidases; Thylakoids | 2020 |
Arsenite suppresses the transcriptional activity of EVI1 through the binding to CCHC-type Zn finger domain.
Topics: Alanine; Amino Acid Substitution; Arsenites; Binding Sites; Cell Nucleus; Complex Mixtures; Cysteine; Dithiothreitol; Electrophoretic Mobility Shift Assay; GATA2 Transcription Factor; Gene Expression Regulation; Humans; K562 Cells; MDS1 and EVI1 Complex Locus Protein; Peptides; Protein Binding; Recombinant Proteins; Signal Transduction; Sodium Compounds; Zinc Fingers | 2020 |
Effect of the drug cyclophosphamide on the activity of porcine kidney betaine aldehyde dehydrogenase.
Topics: Animals; Betaine; Betaine-Aldehyde Dehydrogenase; Catalysis; Catalytic Domain; Chlorine; Cyclophosphamide; Cysteine; Disulfides; Dithiothreitol; Escherichia coli; Kidney; Kinetics; Ligands; Mercaptoethanol; Models, Molecular; Molecular Conformation; Molecular Docking Simulation; Oxidation-Reduction; Oxygen; Pharmaceutical Preparations; Protein Conformation; Reducing Agents; Swine | 2021 |
Oxidative folding of lysozyme with aromatic dithiols, and aliphatic and aromatic monothiols.
Topics: Disulfides; Glutathione Disulfide; Hydrogen-Ion Concentration; Muramidase; Oxidation-Reduction; Protein Folding; Quaternary Ammonium Compounds; Sulfhydryl Compounds | 2012 |
New saccharin derivatives as tyrosinase inhibitors.
Topics: Enzyme Activation; Enzyme Inhibitors; Kinetics; Monophenol Monooxygenase; Musa; Quantum Theory; Saccharin; Software; Structure-Activity Relationship | 2012 |