cysteine has been researched along with biotin in 233 studies
Timeframe | Studies, this research(%) | All Research% |
---|---|---|
pre-1990 | 23 (9.87) | 18.7374 |
1990's | 32 (13.73) | 18.2507 |
2000's | 95 (40.77) | 29.6817 |
2010's | 70 (30.04) | 24.3611 |
2020's | 13 (5.58) | 2.80 |
Authors | Studies |
---|---|
Novogrodsky, A; Wilchek, M; Wynne, D | 1 |
Slyter, LL; Weaver, JM | 1 |
Baggiolini, EG; Confalone, PN; Lollar, D; Pizzolato, G; Uskoković, MR | 2 |
Drexler, H; Kylberg, KJ | 1 |
Bhattacharyya, R; Samanta, TK | 1 |
Deziel, MR; Mau, MM | 1 |
Bayer, EA; Safars, M; Wilchek, M | 1 |
Adamik, R; Bobak, DA; Moss, J; Noda, M; Tsai, SC; Vaughan, M | 1 |
Kison, R; Meyer, HE; Schoner, W | 1 |
Rudman, D; Williams, PJ | 1 |
Drake, HL; Savage, MD | 1 |
Ycas, M | 1 |
Carlsson, J | 2 |
Bal, J; Balbin, E; Pieniazek, NJ | 1 |
Alberts, AW; Nervi, AM; Vagelos, PR | 1 |
Ilahi, M; Kelemen, MV; Rose, AH | 1 |
Duprè, S; Solinas, SP; Spoto, G | 1 |
Frappier, F; Marquet, A | 1 |
Harrell, AC; Jacobson, ES | 1 |
Chen, G; Chilkoti, A; Harris, JM; Hoffman, AS; Long, C; Shimoboji, T; Stayton, PS | 1 |
Capaldi, RA; Tang, C; Wilkens, S | 1 |
Finkelstein, A; Jakes, KS; Qiu, XQ; Slatin, SL | 1 |
Beckwith, J; Guilhot, C; Jander, G; Martin, NL | 1 |
Hendrickson, TL; Imperiali, B; Pathak, R | 1 |
Casey, PJ; Furfine, ES; Landavazo, A; Leban, JJ; Moomaw, JF | 1 |
Cantor, CR; Sano, T; Smith, CL | 1 |
Imai, Y; Komatsubara, S; Masuda, M; Sakurai, N; Tosa, T | 1 |
Bui, BT; Florentin, D; Izumi, Y; Marquet, A; Ohshiro, T; Yamamoto, M | 1 |
Breitling, F; Dübel, S; Kipriyanov, SM; Kontermann, RE; Little, M | 1 |
Bui, BT; Florentin, D; Izumi, Y; Marquet, A; Ohshiro, T | 1 |
Blume, AJ; Brown, AM; Dushin, RG; George, SM; Jacobsen, JS; Sonnenberg-Reines, J | 1 |
Schäfer, H; Schmidt, WE | 1 |
Bui, BT; Florentin, D; Izumi, Y; Marquet, A; Méjean, A; Ploux, O | 1 |
Frillingos, S; Kaback, HR | 1 |
Gravitt, KR; O'Brian, CA; Ward, NE | 1 |
Hubbell, WL; Kaback, HR; Voss, J; Wu, J | 1 |
Baumgartner, R; Buck, GA; Hymes, J; Norrgard, KJ; Pomponio, RJ; Reynolds, TR; Suormala, T; Wolf, B | 1 |
Hagting, A; Konings, WN; Poolman, B; vd Velde, J | 1 |
Cynshi, O; Ikeda, S; Kushima, Y; Sugawa, M; Takashima, Y | 1 |
Achremowicz, B; Udeh, KO | 1 |
Bös, C; Braun, V | 1 |
Chen, JG; Liu-Chen, S; Rudnick, G | 1 |
Asahara, H; Nishikawa, K; Ohtsuka, H; Yokogawa, T | 1 |
Blachly-Dyson, E; Colombini, M; Forte, M; Midson, C; Song, J | 2 |
Amara, SG; Leighton, BH; Seal, RP | 2 |
Boileau, AJ; Czajkowski, C; Davis, AF; Evers, AR | 1 |
Anderluh, G; Barlic, A; Gubensek, F; Macek, P; Menestrina, G; Podlesek, Z; Pungercar, J; Serra, MD; Zecchini, ML | 1 |
Begley, TP; Kinsland, C; McLafferty, F; Taylor, S; Xi, J | 1 |
Asai, Y; Homma, M; Kawagishi, I; Shoji, T | 1 |
Blaustein, RO; Collier, RJ; Finkelstein, A; Gordon, M; Oh, KJ; Senzel, L | 1 |
Levert, KL; Lloyd, RB; Waldrop, GL | 1 |
Bui, BT; Chottard, G; Escalettes, F; Florentin, D; Marquet, A | 1 |
Bures, EJ; Davis, MT; Kroemer, G; McGinley, MD; Patterson, SD; Robinson, JH; Spahr, CS; Susin, SA | 1 |
Kim, JR; Kwon, KS; Lee, SR; Rhee, SG; Yoon, HW | 1 |
Barber, MJ; Pollock, VV | 1 |
Fergusson, MM; Finkel, T; Levine, RL; Sullivan, DM; Wehr, NB | 1 |
Czajkowski, C; Wagner, DA | 1 |
Franke, I; Gast, FU; Meiss, G; Pingoud, A | 1 |
Alving, K; Anderson, DJ; Anderson, GA; Belov, ME; Chrisler, WB; Conrads, TP; Lipton, MS; Pasa-Tolić, L; Smith, RD; Thrall, BD; Udseth, HR; Veenstra, TD | 1 |
Bestagno, M; Burrone, OR; Mandiola, PA; Mukherjee, S; Sepúlveda, J; Vangelista, L | 1 |
Conti, LR; Radeke, CM; Shyng, SL; Vandenberg, CA | 1 |
Marquet, A | 1 |
Casey, JR; Taylor, AM; Zhu, Q | 1 |
Chiang, HJ; Farh, L; Hwang, SY; Shiuan, D; Steinrauf, L | 1 |
Jaffrey, SR; Snyder, SH | 1 |
Gross, SS; Hao, G; Lane, P | 1 |
Ennion, SJ; Evans, RJ | 1 |
Fontecave, M; Hewitson, KS; Münck, E; Ollagnier-de Choudens, S; Roach, P; Sanakis, Y | 1 |
Baldwin, JE; Fontecave, M; Hewitson, KS; Münck, E; Ollagnier-de Choudens, S; Roach, PL; Sanakis, Y; Shaw, NM | 1 |
Eaton, P; Fuller, W; Shattock, MJ | 1 |
Grunewald, M; Kanner, BI; Menaker, D | 1 |
Ferrer, JV; Javitch, JA; Kuhn, DM; Park, SU | 1 |
Donoghue, N; Hogg, PJ; Jiang, XM; Li, P; Matthias, LJ; Poumbourios, P; Vandegraaff, N; Yam, PT | 1 |
Finn, MG; Johnson, JE; Lin, T; Wang, Q | 1 |
Hatsuda, M; Mori, Y; Seki, M; Yamada, S | 1 |
Finkel, E | 1 |
Kobilka, BK; Neumann, L; Whelan, RJ; Wohland, T; Zare, RN | 1 |
Hytönen, VP; Kulomaa, MS; Laitinen, OH; Nordlund, HR; Nyholm, T; Slotte, JP; Uotila, ST | 1 |
Arora, PS; Eisenführ, A; Famulok, M; Nowick, JS; Sengle, G; Takaoka, LR | 1 |
Distefano, MD; Kale, TA | 1 |
Goldknopf, IL; Kone, BC; Kuncewicz, T; Sheta, EA | 1 |
Handel, S; Jose, J | 1 |
Beck-Sickinger, AG; Grouzmann, E; Machova, Z; von Eggelkraut-Gottanka, R | 1 |
Crabtree, M; Gross, SS; Hao, G | 1 |
Flintoff, WF; Sadlish, H; Williams, FM | 1 |
HUNGATE, RE | 1 |
NIMURA, T; SAHASHI, Y; SUZUKI, T | 3 |
Birk, DE; Brittingham, RJ; Colombo, M; Fertala, A; Klement, JF; Majsterek, I; Uitto, J | 1 |
Garfield, S; Gottesman, MM; Liang, XJ; Shen, DW | 1 |
Kim, JE; Tannenbaum, SR | 1 |
Duell, K; Konopka, JB; Lin, JC | 1 |
Chen, GY; Lesaicherre, ML; Lue, RY; Yao, SQ; Zhu, Q | 1 |
Chen, GY; Lesaicherre, ML; Uttamchandani, M; Yao, SQ | 1 |
Chillarón, J; Gasol, E; Jiménez-Vidal, M; Palacín, M; Zorzano, A | 1 |
Conrads, TP; Hood, BL; Kim, BJ; Song, BJ; Suh, SK; Veenstra, TD | 1 |
Hatsuda, M; Mori, Y; Seki, M; Shimizu, T; Yamada, S; Yoshida, S | 1 |
Bolzati, C; Cavazza-Ceccato, M; Marotta, E; Porchia, M; Refosco, F; Tisato, F; Tomasin, P; Traldi, P; Tubaro, M | 1 |
Czajkowski, C; McDevitt, RA; Newell, JG | 1 |
Arbabian, M; Artemyev, NO; Grant, JE; Guo, LW; Hajipour, AR; Muradov, H; Ruoho, AE | 1 |
Greenberg, MM; Sato, K | 1 |
Broniowska, KA; Hogg, N; Keszler, A; Zhang, Y | 1 |
Cataldi, TR; Nardiello, D | 1 |
González de Orduña, C; Higueras, MA; Lamas, S; López-Ferrer, D; Martínez-Ruiz, A; Rodríguez-Crespo, I; Tarín, C; Vázquez, J; Villanueva, L | 1 |
Chen, SS; Yan, W | 1 |
Freeman, ML; Hong, F; Liebler, DC; Sekhar, KR | 1 |
Berzat, AC; Buss, JE; Chenette, EJ; Cox, AD; Der, CJ; Minden, A; Shutes, A; Weinbaum, CA | 1 |
Dahout, C; Gallagher, M; Garin, J; Gonzalez de Peredo, A; Ramus, C | 1 |
Huri, DA; Kim, SF; Snyder, SH | 1 |
Dennehy, MK; Liebler, DC; Richards, KA; Shyr, Y; Wernke, GR | 1 |
Seki, M | 1 |
Adams, DG; Codreanu, SG; Dawson, ES; Liebler, DC; Wadzinski, BE | 1 |
Calder, G; Evans, DJ; Lomonossoff, GP; Steinmetz, NF | 1 |
Marletta, MA; Mitchell, DA; Morton, SU | 1 |
Dangprapai, Y; Pelis, RM; Wright, SH; Wunz, TM | 1 |
Forrester, MT; Foster, MW; Stamler, JS | 1 |
Cheng, P; Gregson, MW; Hackel, BJ; Kapila, A; Lipovsek, D; Lippow, SM; Wittrup, KD | 1 |
Chattopadhaya, S; Tan, LP; Yao, SQ | 1 |
Liebler, DC; Orton, CR | 1 |
Liebler, DC; Liu, Q; Shin, NY; Stamer, SL | 1 |
Anderson, DE; Chen, X; Kirk, KL; Shim, JY; Yang, F; Zhang, X | 1 |
Hanck, DA; Sheets, MF | 1 |
Cummins, TR | 1 |
Agostini, S; Bandoli, G; Bolzati, C; Caporale, A; Carta, D; Cavazza-Ceccato, M; Refosco, F; Schievano, E; Tisato, F | 1 |
Derakhshan, B; Gross, SS; Wille, PC | 1 |
Myers, AG; Siegrist, R; Wulff, JE | 1 |
Eggler, AL; Liu, D; Liu, G; Luo, Y; Mesecar, AD; van Breemen, RB | 1 |
Kamo, N; Kikukawa, T; Kouyama, T; Kurata, Y; Miyauchi, S; Nara, T | 1 |
Gladwin, MT; Hogg, N; Kettenhofen, NJ; Shiva, S; Wang, X | 1 |
Bhakta, S; Eigenbrot, C; Ervin, KE; Junutula, JR; Lowman, HB; Raab, H; Scheller, RH; Vandlen, R | 1 |
Chen, C; Han, P; Huang, B; Zhang, X; Zhou, X | 1 |
Imaizumi, K; Kimura, M; Masuda, Y; Matsuoka, R; Muto, A; Sato, M | 1 |
Fang, J; Iwasa, KH; Marriott, G; Sakata, T | 1 |
Borderie, D; Ekindjian, OG | 1 |
Corjon, S; Engler, T; Kochanek, S; Kreppel, F; van Rooijen, N; Wortmann, A | 1 |
Bachi, A; Malgaroli, A; Torta, F; Usuelli, V | 1 |
Briceño, J; Corrales, FJ; De La Mata, M; Ferrín, G; Gómez, MA; González, R; Hidalgo, AB; López-Cillero, P; López-Sánchez, LM; Muñoz-Castañeda, JR; Muntané, J; Ranchal, I; Rodríguez-Ariza, A | 1 |
Benhar, M; Forrester, MT; Foster, MW; Stamler, JS | 1 |
de la Mata, M; López-Sánchez, LM; Muntané, J; Rodríguez-Ariza, A | 1 |
Campos, SK; Ozbun, MA | 1 |
Yan, LJ | 1 |
Blonski, C; Chaisemartin, L; Chinestra, P; Favre, G; Faye, JC | 1 |
Forrester, MT; Foster, MW; Moseley, MA; Nogueira, L; Stamler, JS; Thompson, JW | 1 |
Abu Bakar, FB; Chattopadhaya, S; Yao, SQ | 1 |
Perkel, JM | 1 |
Bossy, B; Bossy-Wetzel, E; Chen, J; Klinglmayr, E; Knott, AB; Lütz-Meindl, U; Masliah, E; Petrilli, A; Schwarzenbacher, R | 1 |
Crawford, PA; Ivanenkov, VV; Kirley, TL; Sévigny, J; Toyama, A | 1 |
Chandrasena, RE; Edirisinghe, PD; Schiefer, IT; Sinha, V; Thatcher, GR; Wijewickrama, GT; Xu, H | 1 |
Fujishima, SH; Hamachi, I; Nonaka, H; Ojida, A; Uchinomiya, SH | 1 |
Chen, D; Deng, J; Gong, W; Hou, J; Wang, X; Xie, Z; Yang, F; Zhao, Z | 1 |
Chen, Y; Chung, KK; Fu, AK; Fu, WY; Ip, NY; Tsang, AH; Yu, PC; Zhang, P | 1 |
Nishiya, T; Uehara, T | 1 |
Burgoyne, JR; Eaton, P; Mayr, M; Rudyk, O | 1 |
Kazuta, Y; Matsuura, T; Yanagida, H; Yomo, T | 1 |
Branchaud, BP; Ying, LQ | 1 |
Hakalahti, L; Heikkinen, JJ; Hormi, OE; Hytönen, VP; Kivimäki, L; Kulomaa, MS; Määttä, JA; Mäkelä, I; Takkinen, K | 1 |
Burns, RN; Moniri, NH | 1 |
Clarke, DJ; Langridge-Smith, PR; Scotcher, J | 1 |
Brownfield, MS; Elfarra, AA; Irving, RM | 1 |
Ghosal, A; Said, HM | 1 |
Hytönen, VP; Jänis, J; Kulomaa, MS; Määttä, JA; Niederhauser, B; Siivonen, J | 1 |
Cole, RN; Murray, CI; O'Meally, RN; Uhrigshardt, H; Van Eyk, JE | 1 |
Chang, TC; Chien, WT; Jan, FD; Kuo, YY; Liang, CF; Lin, CC; Wu, HT; Yu, CC | 1 |
Inouye, S; Sato, J | 1 |
Bolbach, G; Clavier, S; Diebate, O; Guianvarc'h, D; Karoyan, P; Matheron, L; Sachon, E | 1 |
Chen, X; Cui, L; Guan, T; Kong, J; Li, C; Li, XM; Shang, H; Zhang, X | 1 |
Murray, CI; Van Eyk, JE | 1 |
Bhatnagar, A; Desnoyer, R; Jagannathan, R; Karnik, SS; Tirupula, K; Unal, H | 1 |
Berchner-Pfannschmidt, U; de Groot, H; Fandrey, J; Kirsch, M; Petrat, F; Wiesweg, M | 1 |
Daaka, Y; Dey, A; Qin, Y | 1 |
Gamper, N; Gigout, S; Ooi, L; Pettinger, L | 1 |
Chung, HS; Murray, CI; Uhrigshardt, H; Van Eyk, JE | 1 |
Hirakawa, H; Nagamune, T; Ohashi, H; Oshiba, Y; Tamaki, T; Yamaguchi, S; Yamaguchi, T | 1 |
Kaji, T; Kumagai, Y; Shinkai, Y; Toyama, T | 1 |
Avery, VM; Fechner, GA; Lock, A; Neve, J; Stevenson, GI; Yong, S | 1 |
Bai, L; Cao, Y; Chai, Y; Wang, H; Xiao, L; Yuan, R | 1 |
Ilyas, M; Ilyas, S; Mathur, S; van der Hoorn, RA | 1 |
Ahmed, N; Asif, AR; Khan, R; Oellerich, M; Zahid, S | 1 |
Carroll, KS; Devarie-Baez, NO; Filipovic, MR; Macinkovic, I; Pan, J; Park, CM; Xian, M; Zhang, D | 1 |
Cassia, R; Delena, RA; Lamattina, L; Tavares, CP; Terenzi, H; Vernal, J | 1 |
Fago, A; Gow, AJ; Helbo, S; Howes, BD; Jamil, A; Smulevich, G | 1 |
Ha, HJ; Ha, S; Kim, HJ; Lee, HY; Lee, JJ; Lee, KJ | 1 |
Ohno, K; Okuda, K; Uehara, T | 1 |
Filipovic, MR; Macinkovic, I; Park, CM; Xian, M | 1 |
Paul, BD; Snyder, SH | 1 |
Huang, J; Kast, J; Li, R | 1 |
Aroca, Á; Gotor, C; Romero, LC; Serna, A | 1 |
Gonzalez, P; Green, KN; Rodich, S; Scarborough, JH | 1 |
Carpenter, EP; Liesa, M; Qiu, W; Shirihai, OS | 1 |
Balkan, W; Bomgarden, RD; Chung, HS; Cole, RN; Crowgey, EL; Hare, JM; Kass, DA; Murray, CI; Rainer, PP; Rogers, JC; Van Eyk, JE; Venkatraman, V | 1 |
Fogen, D; Ng, KK; Wong, SL; Wu, SC | 1 |
Gottlieb, CD; Linder, ME; Zhang, S | 1 |
Seth, D; Stamler, JS | 1 |
Liu, WR; Tharp, JM | 1 |
Magwamba, CC; Palittapongarnpim, P; Rukseree, K | 1 |
Akaike, T; Fujii, S; Kasamatsu, S | 1 |
Johnstone, SR; Lohman, AW; Straub, AC | 1 |
Kast, J; Li, R | 1 |
Demizu, Y; Hattori, T; Kurihara, M; Misawa, T; Naito, M; Okitsu, K; Shoda, T | 1 |
Commandeur, U; Hu, H; Le, DHT; Steinmetz, NF | 1 |
de Jong, A; Flierman, D; Kooij, R; Ovaa, H; Witting, K | 1 |
Campuzano, IDG; Kilgour, DPA; Lippens, JL; Loo, JA; Netirojjanakul, C; Nshanian, M; Van Orden, S | 1 |
Bauer, MS; Gaub, HE; Kluger, C; Milles, LF; Pippig, DA; Schendel, LC; Sedlak, SM | 1 |
Chen, X; Cheng, B; Du, Y; Fan, X; Han, M; Hong, W; Huang, R; Liu, Y; Lv, P; Peng, L; Qin, K; Qin, W; Wang, C; Zhou, W; Zhu, Y | 1 |
Cabrera-García, JD; Izquierdo-Álvarez, A; Martínez-Ruiz, A; Tello, D | 1 |
Aimé, S; Hichami, S; Lamotte, O; Wendehenne, D | 1 |
Lindermayr, C; Shekariesfahlan, A | 1 |
Chen, Z; Cole, PA; Dempsey, DR; Jiang, H; Kalin, JH | 1 |
Bai, Y; Fu, Q; Huff-Lonergan, E; Liu, R; Lonergan, S; Xing, L; Zhang, L; Zhang, W; Zhou, G | 1 |
Cheng, R; DeGrado, WF; Li, S; Liu, J; Rozovsky, S; Wang, L; Wang, PG; Wu, H | 1 |
Huang, X; Kroetsch, A; Nguyen, VP; Ogoke, O; Parashurama, N; Park, S; Zhou, F | 1 |
Brun, YV; Dalia, AB; Dalia, TN; Ellison, CK | 1 |
Cook, KM | 1 |
Baranczak, A; Bogdan, AR; Rabalski, AJ | 1 |
Hu, Y; Li, X; Liang, Z; Shan, Y; Weng, Y; Wu, Q; Yuan, H; Zhang, L; Zhang, Y; Zhao, B | 1 |
Hacker, SM; Lewald, L; Zanon, PRA | 1 |
Abbott, D; Arvan, P; Bederman, I; Cameron, M; Chance, M; Chirieleison, SM; Gao, XH; Gao, Z; Hatzoglou, M; Krokowski, D; Li, L; Parisien, M; Wang, B; Willard, B; Wu, J | 1 |
Shusta, EV; Umlauf, BJ | 1 |
Chen, C; Chen, X; Gong, W; Hu, W; Liu, Z; Perrett, S; Wang, L; Wu, H; Wu, S; Yang, J; Zhang, H | 1 |
Berg, EA; Fishman, JB | 1 |
Backus, KM; Boatner, LM; Cao, J; Desai, HS; Jami-Alahmadi, Y; Palafox, MF; Yan, T; Yen, SL | 1 |
Diao, H; Jiang, N; Li, R; Pan, H; Qi, Q; Sun, S; Wan, X; Wang, J; Zhang, F; Zhang, L; Zhang, M | 1 |
Brandão-Neto, J; D'Muniz Pereira, H; Martins, PGA; Menegatti, ACO; Rocha, RF; Terenzi, H; Thiemann, OH | 1 |
Huang, D; Li, X; Liu, D; Liu, J; Liu, Y; Luo, K; Mai, K; Pan, M; Wu, Z; Zhang, W | 1 |
Hunter, R; Kaschula, CH; Katz, AA; Kusza, DA; Schäfer, G; Smith, M | 1 |
Bai, Q; Ma, G; Wang, H | 1 |
Chen, Z; Cheng, X; Jiang, Z; Liu, G; Niu, Y; Tang, B; Tong, L; Yang, Y; Zhang, G | 1 |
Aucagne, V; Berger, S; Kersaudy, F; Lefoulon, F; Morisset-Lopez, S; Novak, A; Pifferi, C | 1 |
13 review(s) available for cysteine and biotin
Article | Year |
---|---|
Nutrient deficiencies during total parenteral nutrition.
Topics: Adolescent; Adult; Aged; Animals; Biotin; Carnitine; Child; Choline Deficiency; Chromium; Copper; Cysteine; Deficiency Diseases; Dogs; Fatty Acids, Essential; Female; Folic Acid Deficiency; Guinea Pigs; Humans; Infant; Male; Middle Aged; Molybdenum; Parenteral Nutrition; Parenteral Nutrition, Total; Phosphates; Rats; Selenium; Sheep; Taurine; Tyrosine; Vitamin A Deficiency; Zinc | 1985 |
The enzymology of sulfur activation during thiamin and biotin biosynthesis.
Topics: Biotin; Cysteine; Models, Chemical; Phosphotransferases (Alcohol Group Acceptor); Sulfur; Sulfurtransferases; Thiamine | 1999 |
Enzymology of carbon-sulfur bond formation.
Topics: Acetyltransferases; Anaerobiosis; Bacterial Proteins; Biotin; Cysteine; Enzymes; Intramolecular Transferases; Iron-Sulfur Proteins; Models, Chemical; S-Adenosylmethionine; Sulfur; Sulfurtransferases | 2001 |
S-nitrosylation is emerging as a specific and fundamental posttranslational protein modification: head-to-head comparison with O-phosphorylation.
Topics: Animals; Argininosuccinate Synthase; Biotin; Biotinylation; Cysteine; Humans; Nitric Oxide; Phosphorylation; Protein Processing, Post-Translational; Signal Transduction; Spectrometry, Mass, Electrospray Ionization | 2001 |
Mass spectrometry-based quantitative proteomic profiling.
Topics: Biotin; Cysteine; Electrophoresis, Gel, Two-Dimensional; Ions; Mass Spectrometry; Peptides; Protein Structure, Tertiary; Proteomics; Software; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2005 |
Biological significance and development of practical synthesis of biotin.
Topics: Animals; Avidin; Biotin; Catalysis; Cysteine; Hypoglycemic Agents; Insulin; Insulin Secretion; Iron; Lactones; Models, Biological; Models, Chemical; Pimelic Acids; Rhizobium | 2006 |
Assessment of the nitrosylation process.
Topics: Biotin; Blotting, Western; Colorimetry; Cysteine; Luminescent Measurements; Mass Spectrometry; Nitric Oxide; Nitrosation; Proteins; Spectrometry, Fluorescence; Sulfhydryl Reagents | 2008 |
Proteomic analysis of protein S-nitrosylation.
Topics: Animals; Biotin; Cysteine; Electrophoresis, Gel, Two-Dimensional; Humans; Mass Spectrometry; Nitric Oxide; Protein Processing, Post-Translational; Proteins; Proteomics; Signal Transduction | 2008 |
Detection of protein S-nitrosylation with the biotin-switch technique.
Topics: Animals; Biotin; Cell Extracts; Chemistry Techniques, Analytical; Cysteine; Humans; Nitric Oxide; Oxidation-Reduction; Protein Processing, Post-Translational; S-Nitrosothiols; Signal Transduction; Sulfhydryl Compounds | 2009 |
Unraveling the S-nitrosoproteome: tools and strategies.
Topics: Animals; Biotin; Cell Communication; Cysteine; Humans; Mice; Nitric Oxide; Nitrosation; Oxidation-Reduction; Protein Processing, Post-Translational; Proteins; Proteomics; Rats; S-Nitrosothiols; Sulfhydryl Compounds | 2009 |
Screening systems for the identification of S-nitrosylated proteins.
Topics: Binding Sites; Biotin; Computational Biology; Cysteine; Disulfides; Mesylates; Nitric Oxide; Oxidation-Reduction; Protein Array Analysis; Protein Processing, Post-Translational; Proteins; Proteomics; S-Nitrosothiols; Substrate Specificity | 2011 |
Protein s-nitrosylation measurement.
Topics: Antibodies; Biotin; Cysteine; Humans; Luminescent Measurements; Mass Spectrometry; Nitric Oxide; Protein Processing, Post-Translational; Receptors, G-Protein-Coupled; S-Nitrosothiols; Signal Transduction; Staining and Labeling | 2013 |
The biological functions of protein S-sulfhydration in eukaryotes and the ever-increasing understanding of its effects on bacteria.
Topics: Animals; Bacteria; Biotin; Cysteine; Eukaryota; Hydrogen Sulfide; Mammals; Protein Processing, Post-Translational; Protein S | 2023 |
220 other study(ies) available for cysteine and biotin
Article | Year |
---|---|
A chemical approach for the localization of membrane sites involved in lymphocyte activation.
Topics: Animals; Avidin; Binding Sites; Biotin; Cell Aggregation; Cell Membrane; Cysteine; Dinitrophenols; DNA; DNA Replication; Hydrazines; Lymphocyte Activation; Mice; Mice, Inbred Strains; Mitogens; Periodic Acid; T-Lymphocytes | 1976 |
Tetrahydrofolate and other growth requirements of certain strains of Ruminococcus flavefaciens.
Topics: Acetates; Amino Acids; Ammonia; Animals; Biotin; Butyrates; Carbon Dioxide; Cattle; Culture Media; Cysteine; Peptococcaceae; Rumen; Tetrahydrofolates; Thiamine; Valerates | 1977 |
Stereospecific total synthesis of d-biotin from L(+)-cysteine.
Topics: Biotin; Cysteine; Methods; Stereoisomerism | 1977 |
Effect of UV irradiation on transduction by coliphage T1.
Topics: Arginine; Biotin; Coliphages; Cysteine; DNA Viruses; Drug Resistance, Microbial; Escherichia coli; Galactose; Genetic Linkage; Lactose; Lysogeny; Mutation; Proline; Radiation Effects; Streptomycin; Transduction, Genetic; Tryptophan; Ultraviolet Rays | 1975 |
Letter: A'stereospecific total synthesis of d-biotin from L-(+)-cysteine.
Topics: Biotin; Cysteine; Stereoisomerism | 1975 |
L-lysine production by S-2-aminoethyl-L-cysteine-resistant mutants of Arthrobacter globiformis.
Topics: Arthrobacter; Biotin; Culture Media; Cysteine; Drug Resistance, Microbial; Glucose; Lysine; Methylnitronitrosoguanidine; Mutagenesis; Mutation; Nitrogen; Species Specificity | 1991 |
Biotin-conjugated reagents as site-specific probes of membrane protein structure: application to the study of the human erythrocyte hexose transporter.
Topics: Biotin; Carrier Proteins; Cysteine; Erythrocyte Membrane; Galactose Oxidase; Hexoses; Humans; Hydrazines; Hydrolysis; Maleimides; Membrane Proteins; Serine Endopeptidases; Trypsin | 1990 |
Selective labeling of sulfhydryls and disulfides on blot transfers using avidin-biotin technology: studies on purified proteins and erythrocyte membranes.
Topics: Avidin; Biotin; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Erythrocyte Membrane; Humans; Lysine; Maleimides; Membrane Proteins; Mercaptoethanol; Proteins; Staining and Labeling; Sulfhydryl Compounds | 1987 |
Activation of immobilized, biotinylated choleragen AI protein by a 19-kilodalton guanine nucleotide-binding protein.
Topics: Biotin; Cholera Toxin; Cysteine; Enzyme Activation; GTP-Binding Proteins; Molecular Weight; NAD; Poly(ADP-ribose) Polymerases | 1989 |
Characterization of a cysteine-containing peptide after affinity labelling of Ca2+-ATPase of sarcoplasmic reticulum with the disulfide of 3'(2')-O-biotinyl-thioinosine triphosphate.
Topics: Affinity Labels; Amino Acid Sequence; Animals; Biotin; Calcium; Calcium-Transporting ATPases; Cysteine; Kinetics; Molecular Sequence Data; Peptide Fragments; Rabbits; Sarcoplasmic Reticulum; Thionucleotides; Trypsin | 1989 |
Adaptation of the acetogen Clostridium thermoautotrophicum to minimal medium.
Topics: Acetates; Acetic Acid; Adaptation, Physiological; Biotin; Clostridium; Culture Media; Cysteine; Glucose; Methanol; Niacin | 1986 |
On earlier states of the biochemical system.
Topics: Amino Acid Sequence; Bacillus cereus; Bacterial Proteins; Biological Evolution; Biotin; Coenzyme A; Cysteine; Enzymes; Escherichia coli; Genetic Code; Glucosephosphate Dehydrogenase; Ketoglutarate Dehydrogenase Complex; Methionine; Molecular Biology; Multienzyme Complexes; Mutation; Penicillins; Peptide Chain Termination, Translational; Pyruvate Dehydrogenase Complex | 1974 |
Nutritional requirements of Streptococcus sanguis.
Topics: Biotin; Culture Media; Cysteine; Glucose; Mouth; Nicotinic Acids; Pantothenic Acid; Pyridoxine; Riboflavin; Spectrophotometry; Streptococcus; Thiamine; Tooth | 1972 |
Method for isolating auxotrophic mutants in Aspergillus nidulans using N-glycosyl-polifungin.
Topics: Aminobenzoates; Antifungal Agents; Aspergillus nidulans; Biotin; Cell Survival; Cysteine; Mutation; Nucleic Acids; Polyenes; Spores, Fungal; Ultraviolet Rays | 1974 |
Acetyl CoA carboxylase. 3. Purification and properties of a biotin carboxyl carrier protein.
Topics: Amino Acids; Bacterial Proteins; Benzoates; Biotin; Catalysis; Chemical Phenomena; Chemistry; Chemistry, Physical; Chromatography, DEAE-Cellulose; Chromatography, Gel; Coenzyme A; Crystallization; Cysteine; Detergents; Diffusion; Electrophoresis, Disc; Escherichia coli; Ethanol; Ligases; Methods; Protein Binding; Protein Denaturation; Quaternary Ammonium Compounds; Sulfates; Sulfhydryl Compounds; Sulfides; Sulfuric Acids; Ultracentrifugation; Urea | 1971 |
Studies on the biosynthesis of biotin. Production of biotin and biotin-like compounds by a pseudomonad.
Topics: Amino Acids; Biotin; Chromatography, Paper; Culture Media; Cysteine; Cystine; In Vitro Techniques; Lysine; Malonates; Pimelic Acids; Pseudomonas aeruginosa | 1965 |
Nutritional requirements of Streptococcus salivarius.
Topics: Ammonia; Biotin; Culture Media; Cysteine; Cystine; Glucose; Glutamates; Homocysteine; Homocystine; Humans; Mouth; Nicotinic Acids; Pantothenic Acid; Riboflavin; Streptococcus; Thiamine; Urea | 1971 |
Cysteine as precursor and sulfur donor in the biosynthesis of natural sulfur-containing compounds.
Topics: Animals; Biotin; Cysteine; Ergothioneine; Glutathione; Thiamine; Thioctic Acid; Tissue Distribution | 1983 |
On the biosynthesis of biotin in Achromobacter IVSW. A reinvestigation.
Topics: Alcaligenes; Biotin; Carbon Radioisotopes; Cysteine; Radioisotope Dilution Technique; Sulfur Radioisotopes; Tritium | 1981 |
A prototrophic yeast-strain of Histoplasma capsulatum.
Topics: Animals; Biotin; Culture Media; Cysteine; Glucose; Histoplasma; Histoplasmosis; Mice; Sodium; Sulfates | 1982 |
Control of protein-ligand recognition using a stimuli-responsive polymer.
Topics: Acrylic Resins; Bacterial Proteins; Biotin; Cysteine; Cytochromes b5; Ligands; Models, Molecular; Mutagenesis, Site-Directed; Polymers; Protein Binding; Protein Conformation; Recombinant Proteins; Streptavidin; Sulfones; Temperature | 1995 |
Structure of the gamma subunit of Escherichia coli F1 ATPase probed in trypsin digestion and biotin-avidin binding studies.
Topics: Amino Acid Sequence; Antibodies, Monoclonal; Avidin; Biotin; Cysteine; Epitopes; Molecular Sequence Data; Peptide Mapping; Protein Structure, Secondary; Proton-Translocating ATPases; Structure-Activity Relationship; Trypsin | 1994 |
Identification of a translocated protein segment in a voltage-dependent channel.
Topics: Amino Acid Sequence; Bacterial Proteins; Biological Transport; Biotin; Colicins; Cysteine; Electrophysiology; Ion Channel Gating; Ion Channels; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Protein Conformation; Streptavidin | 1994 |
Evidence that the pathway of disulfide bond formation in Escherichia coli involves interactions between the cysteines of DsbB and DsbA.
Topics: Bacterial Proteins; Biotin; Blotting, Western; Cell Fractionation; Cysteine; Disulfides; Escherichia coli; Genes, Bacterial; Genes, Dominant; Isomerases; Membrane Proteins; Mutagenesis; Oxidation-Reduction; Protein Binding; Protein Disulfide-Isomerases; Recombinant Proteins | 1995 |
Sulfhydryl modification of the yeast Wbp1p inhibits oligosaccharyl transferase activity.
Topics: Amino Acid Sequence; Biotin; Carbohydrate Sequence; Catalysis; Cysteine; Dithiothreitol; Enzyme Stability; Hexosyltransferases; Kinetics; Macromolecular Substances; Membrane Proteins; Mesylates; Methyl Methanesulfonate; Microsomes; Molecular Sequence Data; Polyisoprenyl Phosphate Oligosaccharides; Saccharomyces cerevisiae; Structure-Activity Relationship; Sulfhydryl Compounds; Transferases | 1995 |
Protein farnesyltransferase: kinetics of farnesyl pyrophosphate binding and product release.
Topics: Alkyl and Aryl Transferases; Amino Acid Sequence; Binding Sites; Biotin; Catalysis; Cysteine; Kinetics; Molecular Sequence Data; Peptides; Polyisoprenyl Phosphates; Protein Prenylation; Sesquiterpenes; Spectrometry, Fluorescence; Transferases | 1995 |
A streptavidin mutant containing a cysteine stretch that facilitates production of a variety of specific streptavidin conjugates.
Topics: Amino Acid Sequence; Animals; Bacterial Proteins; Base Sequence; Biotin; Cell Membrane; Cloning, Molecular; Cysteine; DNA, Recombinant; Escherichia coli; Fluoresceins; Genetic Vectors; Methylation; Mice; Molecular Sequence Data; Streptavidin; Sulfhydryl Compounds; Tumor Cells, Cultured | 1993 |
Improvement of a d-biotin-hyperproducing recombinant strain of Serratia marcescens.
Topics: Biotin; Cysteine; Ethionine; Plasmids; Recombinant Proteins; Serratia marcescens | 1994 |
Stimulatory factors for enzymatic biotin synthesis from dethiobiotin in cell-free extracts of Escherichia coli.
Topics: Bacillus; Biotin; Cell Extracts; Cysteine; Escherichia coli; Flavin-Adenine Dinucleotide; Immunosuppressive Agents; NAD; NADP; S-Adenosylmethionine | 1995 |
Recombinant single-chain Fv fragments carrying C-terminal cysteine residues: production of bivalent and biotinylated miniantibodies.
Topics: Amino Acid Sequence; Animals; Antibodies, Bispecific; Antibody Affinity; Base Sequence; Biotin; Blotting, Western; Chromatography, Affinity; Cysteine; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Immunoglobulin Fragments; Immunoglobulin Variable Region; Mice; Molecular Sequence Data; Recombinant Proteins | 1994 |
On the mechanism of biotin synthase of Bacillus sphaericus.
Topics: Bacillus; Biotin; Cysteine; S-Adenosylmethionine; Sulfurtransferases | 1994 |
Biotinylated and cysteine-modified peptides as useful reagents for studying the inhibition of cathepsin G.
Topics: alpha 1-Antichymotrypsin; Amino Acid Sequence; Biotin; Cathepsin G; Cathepsins; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Peptides; Reproducibility of Results; Sensitivity and Specificity; Serine Endopeptidases; Tritium | 1994 |
Characterization and purification of the solubilized pituitary adenylate-cyclase-activating polypeptide-1 receptor from porcine brain using a biotinylated ligand.
Topics: Animals; Biotin; Brain; Chromatography, Affinity; Cysteine; Electrophoresis, Polyacrylamide Gel; Glycosylation; Ligands; Neuropeptides; Radioligand Assay; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; Receptors, Pituitary Hormone; Swine | 1993 |
Highly purified biotin synthase can transform dethiobiotin into biotin in the absence of any other protein, in the presence of photoreduced deazaflavin.
Topics: Amino Acid Sequence; Bacillus; Biotin; Cell-Free System; Cysteine; Flavins; Molecular Sequence Data; Spectrum Analysis; Sulfur; Sulfurtransferases | 1995 |
Probing the conformation of the lactose permease of Escherichia coli by in situ site-directed sulfhydryl modification.
Topics: Amino Acid Sequence; Biotin; Carbohydrate Sequence; Cell Membrane; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Kinetics; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Plasmids; Point Mutation; Protein Conformation; Recombinant Proteins; Sulfhydryl Compounds; Symporters; Thiogalactosides; Valine | 1996 |
Covalent modification of protein kinase C isozymes by the inactivating peptide substrate analog N-biotinyl-Arg-Arg-Arg-Cys-Leu-Arg-Arg-Leu. Evidence that the biotinylated peptide is an active-site affinity label.
Topics: Animals; Binding Sites; Biotin; Brain; Calcium; Cysteine; Dithiothreitol; Enzyme Inhibitors; Isoenzymes; Oligopeptides; Phosphatidylserines; Protein Binding; Protein Kinase C; Rats | 1996 |
Site-directed spin labeling and chemical crosslinking demonstrate that helix V is close to helices VII and VIII in the lactose permease of Escherichia coli.
Topics: Amino Acid Sequence; Biotin; Cell Membrane; Cysteine; Cytoplasm; Electron Spin Resonance Spectroscopy; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Membrane Transport Proteins; Models, Structural; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Recombinant Proteins; Spin Labels; Symporters | 1996 |
Arg538 to Cys mutation in a CpG dinucleotide of the human biotinidase gene is the second most common cause of profound biotinidase deficiency in symptomatic children.
Topics: Acyltransferases; Alleles; Amidohydrolases; Antibodies; Arginine; Automation; Biotin; Biotinidase; Cells, Cultured; Child; Cysteine; Dinucleoside Phosphates; DNA; Exons; Female; Fibroblasts; Humans; Male; Pedigree; Point Mutation; Sequence Analysis, DNA | 1997 |
Membrane topology of the di- and tripeptide transport protein of Lactococcus lactis.
Topics: Alkaline Phosphatase; Amino Acid Sequence; Animals; Bacterial Proteins; Base Sequence; Biotin; Carrier Proteins; Cell Membrane; Cysteine; Dipeptides; Escherichia coli; Humans; Lactococcus lactis; Membrane Transport Proteins; Models, Chemical; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptides; Plasmids; Polymerase Chain Reaction; Protein Structure, Secondary; Recombinant Fusion Proteins; Sequence Alignment | 1997 |
Oxidized low density lipoprotein caused CNS neuron cell death.
Topics: Animals; Antidotes; Antioxidants; Biotin; Cell Death; Cells, Cultured; Cerebral Cortex; Cysteine; Deoxyuracil Nucleotides; Dithiothreitol; DNA Fragmentation; Female; Fetus; Glutathione; Hippocampus; Humans; Lipoproteins, LDL; Neuroblastoma; Oxidation-Reduction; Oxidative Stress; Rabbits; Rats; Rats, Wistar; Staining and Labeling; Sulfhydryl Reagents; Thiobarbituric Acid Reactive Substances; Tumor Cells, Cultured; Vitamin E | 1997 |
High-glutathione containing yeast Saccharomyces cerevisiae: optimization of production.
Topics: Biomass; Biotin; Culture Media; Cysteine; Glucose; Glutathione; Peptones; Phosphates; Potassium Compounds; Saccharomyces cerevisiae | 1997 |
Specific in vivo thiol-labeling of the FhuA outer membrane ferrichrome transport protein of Escherichia coli K-12: evidence for a disulfide bridge in the predicted gating loop.
Topics: Amino Acid Sequence; Bacterial Outer Membrane Proteins; Biotin; Cysteine; Disulfides; Escherichia coli; Escherichia coli Proteins; Ferrichrome; Hot Temperature; Ligands; Maleimides; Molecular Sequence Data; Mutation; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Receptors, Virus; Sulfhydryl Reagents | 1997 |
Determination of external loop topology in the serotonin transporter by site-directed chemical labeling.
Topics: Biotin; Carrier Proteins; Cysteine; Lysine; Membrane Glycoproteins; Membrane Transport Proteins; Mutagenesis, Site-Directed; Nerve Tissue Proteins; Protein Conformation; Serotonin Plasma Membrane Transport Proteins; Succinimides | 1998 |
Use of biotinylated-cysteinyl-tRNA as a non-RI probe in protein synthesis.
Topics: Biotin; Cell-Free System; Cysteine; Escherichia coli; Models, Molecular; Protein Biosynthesis; Protein Conformation; Proteins; RNA, Messenger; RNA, Transfer, Amino Acyl; RNA, Transfer, Cys | 1997 |
The sensor regions of VDAC are translocated from within the membrane to the surface during the gating processes.
Topics: Amino Acid Sequence; Amino Acid Substitution; Biotin; Cysteine; Electric Conductivity; Electrophysiology; Intracellular Membranes; Ion Channel Gating; Lipid Bilayers; Membrane Potentials; Membrane Proteins; Mitochondria; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Neurospora crassa; Porins; Protein Conformation; Protein Folding; Recombinant Proteins; Saccharomyces cerevisiae; Streptavidin; Voltage-Dependent Anion Channels | 1998 |
The topology of VDAC as probed by biotin modification.
Topics: Amino Acid Sequence; Binding Sites; Biotin; Biotinylation; Cysteine; Electrophysiology; Ion Channel Gating; Liposomes; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Neurospora crassa; Point Mutation; Porins; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Streptavidin; Voltage-Dependent Anion Channels | 1998 |
Transmembrane topology mapping using biotin-containing sulfhydryl reagents.
Topics: Animals; Biotin; Biotinylation; Blotting, Western; Cell Membrane; Cells, Cultured; Cysteine; Electrophoresis, Polyacrylamide Gel; Indicators and Reagents; Membrane Proteins; Models, Molecular; Mutagenesis, Site-Directed; Protein Structure, Secondary; Recombinant Proteins; Sulfhydryl Reagents; Transfection | 1998 |
Mapping the agonist binding site of the GABAA receptor: evidence for a beta-strand.
Topics: Animals; Binding Sites; Biotin; Chromosome Mapping; Cysteine; Dose-Response Relationship, Drug; Ethyl Methanesulfonate; GABA Agonists; gamma-Aminobutyric Acid; Humans; Indicators and Reagents; Kidney; Membrane Potentials; Models, Molecular; Molecular Sequence Data; Muscimol; Mutagenesis, Site-Directed; Oocytes; Protein Structure, Tertiary; Receptors, GABA-A; Sequence Homology, Amino Acid; Tritium; Xenopus | 1999 |
Cysteine-scanning mutagenesis of an eukaryotic pore-forming toxin from sea anemone: topology in lipid membranes.
Topics: 2-Naphthylamine; Amino Acid Sequence; Amino Acid Substitution; Animals; Avidin; Binding Sites; Biotin; Cloning, Molecular; Cnidarian Venoms; Cysteine; Liposomes; Membrane Lipids; Models, Molecular; Molecular Probes; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Sea Anemones; Solutions | 1999 |
Cysteine-scanning mutagenesis of the periplasmic loop regions of PomA, a putative channel component of the sodium-driven flagellar motor in Vibrio alginolyticus.
Topics: Amino Acid Sequence; Amino Acid Substitution; Bacterial Outer Membrane Proteins; Biotin; Cysteine; Dithiothreitol; Electroporation; Flagella; Maleimides; Molecular Sequence Data; Mutagenesis; Protein Structure, Secondary; Sodium; Vibrio | 2000 |
Topography of diphtheria Toxin's T domain in the open channel state.
Topics: Amino Acid Sequence; Amino Acid Substitution; Biotin; Cell Membrane; Cysteine; Diphtheria Toxin; Humans; Ion Channel Gating; Ion Channels; Lipid Bilayers; Maleimides; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Streptavidin | 2000 |
Do cysteine 230 and lysine 238 of biotin carboxylase play a role in the activation of biotin?
Topics: Animals; Binding Sites; Biotin; Carbon-Nitrogen Ligases; Catalysis; Cysteine; Lysine; Mutagenesis, Site-Directed; Substrate Specificity | 2000 |
A model for the topology of excitatory amino acid transporters determined by the extracellular accessibility of substituted cysteines.
Topics: Amino Acid Sequence; Amino Acid Transport System X-AG; Amino Acids; Animals; ATP-Binding Cassette Transporters; Biotin; COS Cells; Cysteine; Ethyl Methanesulfonate; Extracellular Space; Humans; Indicators and Reagents; Ion Channel Gating; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Tertiary | 2000 |
Enzyme-mediated sulfide production for the reconstitution of [2Fe-2S] clusters into apo-biotin synthase of Escherichia coli. Sulfide transfer from cysteine to biotin.
Topics: Bacterial Proteins; Biotin; Cysteine; Escherichia coli; Iron-Sulfur Proteins; Sulfides; Sulfurtransferases | 2000 |
Simplification of complex peptide mixtures for proteomic analysis: reversible biotinylation of cysteinyl peptides.
Topics: Animals; Biotin; Biotinylation; Cysteine; Humans; Mitochondria; Peptides; Proteome | 2000 |
Identification of proteins containing cysteine residues that are sensitive to oxidation by hydrogen peroxide at neutral pH.
Topics: Alkylation; Amino Acid Sequence; Animals; Biotin; Creatine Kinase; Cysteine; Ethylenediamines; Hydrogen Peroxide; Hydrogen-Ion Concentration; Molecular Sequence Data; Oxidation-Reduction; PC12 Cells; Proteins; Rabbits; Rats; Streptavidin | 2000 |
Serine 121 is an essential amino acid for biotin sulfoxide reductase functionality.
Topics: Alanine; Amino Acid Sequence; Amino Acids; Binding Sites; Biotin; Catalysis; Chromatography, High Pressure Liquid; Coenzymes; Conserved Sequence; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Factor Xa; Glutathione Transferase; Guanine Nucleotides; Iron-Sulfur Proteins; Ligands; Mass Spectrometry; Metalloproteins; Molecular Sequence Data; Molybdenum Cofactors; Mutagenesis, Site-Directed; NADP; Oxidative Stress; Oxidoreductases; Oxygen; Paraquat; Pteridines; Pterins; Recombinant Fusion Proteins; Rhodobacter sphaeroides; Sequence Homology, Amino Acid; Serine; Spectrometry, Fluorescence; Threonine; Time Factors | 2000 |
Identification of oxidant-sensitive proteins: TNF-alpha induces protein glutathiolation.
Topics: Animals; Annexin A2; Biotin; Cattle; Cysteine; Dose-Response Relationship, Drug; Glutathione; Glyceraldehyde-3-Phosphate Dehydrogenases; HeLa Cells; Humans; Hydrogen Peroxide; Neoplasm Proteins; Oxidants; Peroxidases; Peroxiredoxin III; Peroxiredoxins; Proteins; Reactive Oxygen Species; Solubility; Succinimides; Tumor Necrosis Factor-alpha | 2000 |
Structure and dynamics of the GABA binding pocket: A narrowing cleft that constricts during activation.
Topics: Animals; Binding Sites; Biotin; Cells, Cultured; Cysteine; Dose-Response Relationship, Drug; GABA Agonists; GABA Antagonists; GABA Modulators; gamma-Aminobutyric Acid; Ion Channel Gating; Ligands; Mutagenesis, Site-Directed; Oocytes; Patch-Clamp Techniques; Protein Structure, Tertiary; Rats; Receptors, GABA-A; Structure-Activity Relationship; Sulfhydryl Reagents | 2001 |
Immobilization of sugar-non-specific nucleases by utilizing the streptavidin--biotin interaction.
Topics: Amino Acid Sequence; Base Sequence; Biotin; Carbohydrate Metabolism; Chromatography, Gel; Codon; Cysteine; Endodeoxyribonucleases; Endonucleases; Endoribonucleases; Enzymes, Immobilized; Molecular Sequence Data; Phosphoric Diester Hydrolases; Protein Engineering; Receptors, Growth Factor; Streptavidin; Surface Plasmon Resonance; Ultrafiltration | 2001 |
Quantitative analysis of bacterial and mammalian proteomes using a combination of cysteine affinity tags and 15N-metabolic labeling.
Topics: Animals; Avidin; Bacteria; Biotin; Cells, Cultured; Chromatography, Affinity; Chromatography, Liquid; Cysteine; Melanoma, Experimental; Mice; Nitrogen Isotopes; Peptide Mapping; Peptides; Proteome; Spectrometry, Mass, Electrospray Ionization; Spectroscopy, Fourier Transform Infrared; Trypsin | 2001 |
Membrane immunoglobulins are stabilized by interchain disulfide bonds occurring within the extracellular membrane-proximal domain.
Topics: Amino Acid Sequence; Biotin; Cysteine; Disulfides; Humans; Molecular Sequence Data; Mutation; Receptors, Antigen, B-Cell; Sequence Alignment | 2001 |
Transmembrane topology of the sulfonylurea receptor SUR1.
Topics: Animals; ATP-Binding Cassette Transporters; Biotin; COS Cells; Cysteine; Models, Molecular; Mutagenesis; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Inwardly Rectifying; Receptors, Drug; Sulfonylurea Receptors | 2001 |
Cysteine-directed cross-linking localizes regions of the human erythrocyte anion-exchange protein (AE1) relative to the dimeric interface.
Topics: Amino Acid Sequence; Anion Exchange Protein 1, Erythrocyte; Biotin; Cell Line; Cross-Linking Reagents; Cysteine; Dimerization; Humans; Maleimides; Molecular Sequence Data; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Transfection | 2001 |
Structure-function studies of Escherichia coli biotin synthase via a chemical modification and site-directed mutagenesis approach.
Topics: Amino Acid Sequence; Binding Sites; Biotin; Cysteine; Dimerization; Enzyme Activation; Escherichia coli; Iron-Sulfur Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Sequence Homology, Amino Acid; Structure-Activity Relationship; Sulfurtransferases | 2001 |
The biotin switch method for the detection of S-nitrosylated proteins.
Topics: Animals; Biotin; Biotinylation; Cysteine; False Positive Reactions; Humans; Immunoblotting; Proteins; S-Nitrosothiols; Signal Transduction | 2001 |
Conserved cysteine residues in the extracellular loop of the human P2X(1) receptor form disulfide bonds and are involved in receptor trafficking to the cell surface.
Topics: Adenosine Triphosphate; Alanine; Amino Acid Sequence; Amino Acid Substitution; Animals; Biological Transport; Biotin; Biotinylation; Conserved Sequence; Cysteine; Disulfides; Dithiothreitol; Electrophysiology; Humans; Membrane Proteins; Mercaptoethanol; Molecular Sequence Data; Mutation; Oocytes; Protein Structure, Tertiary; Receptors, Purinergic P2; Receptors, Purinergic P2X; Reducing Agents; Sequence Homology, Amino Acid; Xenopus laevis | 2002 |
Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli.
Topics: Biotin; Cysteine; Electron Spin Resonance Spectroscopy; Escherichia coli; Kinetics; Methionine; Models, Chemical; Mutation; S-Adenosylmethionine; Spectroscopy, Mossbauer; Sulfurtransferases; Time Factors | 2002 |
The iron-sulfur center of biotin synthase: site-directed mutants.
Topics: Acetyltransferases; Amino Acid Motifs; Amino Acid Substitution; Biotin; Cysteine; Electron Spin Resonance Spectroscopy; Enzymes; Escherichia coli; Intramolecular Transferases; Iron; Iron Chelating Agents; Iron-Sulfur Proteins; Mutagenesis, Site-Directed; Oxidation-Reduction; Sulfurtransferases | 2002 |
S-thiolation of HSP27 regulates its multimeric aggregate size independently of phosphorylation.
Topics: Animals; Biotin; Biotinylation; Blotting, Western; Cysteine; Enzyme Inhibitors; Heart; Heat-Shock Proteins; HSP27 Heat-Shock Proteins; Hydrogen Peroxide; Male; Neoplasm Proteins; Oxidative Stress; Perfusion; Phosphorylation; Rats; Rats, Wistar; Serine; Sulfhydryl Compounds; Time Factors | 2002 |
Cysteine-scanning mutagenesis reveals a conformationally sensitive reentrant pore-loop in the glutamate transporter GLT-1.
Topics: Amino Acid Substitution; Biotin; Cysteine; Excitatory Amino Acid Transporter 2; Glutamic Acid; HeLa Cells; Humans; Indicators and Reagents; Kainic Acid; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Protein Structure, Secondary; Sodium; Structure-Activity Relationship | 2002 |
Peroxynitrite inactivates the human dopamine transporter by modification of cysteine 342: potential mechanism of neurotoxicity in dopamine neurons.
Topics: Antioxidants; Biological Transport; Biotin; Cell Line; Cysteine; Dopamine; Dopamine Plasma Membrane Transport Proteins; Dose-Response Relationship, Drug; Free Radical Scavengers; Humans; Kidney; Membrane Glycoproteins; Membrane Transport Modulators; Membrane Transport Proteins; Mutagenesis, Site-Directed; Nerve Tissue Proteins; Neurons; Neurotoxicity Syndromes; Peroxynitrous Acid; Structure-Activity Relationship; Substrate Specificity; Sulfhydryl Reagents; Transfection | 2002 |
Disulfide exchange in domain 2 of CD4 is required for entry of HIV-1.
Topics: Arsenicals; Biotin; CD4 Antigens; CD4-Positive T-Lymphocytes; Cysteine; Glutathione; HIV-1; Humans; Immunoblotting; Lysine; Maleimides; Mutation; Oxidation-Reduction; Protein Structure, Tertiary; Receptors, HIV; Succinimides; Sulfhydryl Compounds; Thioredoxins | 2002 |
Natural supramolecular building blocks. Cysteine-added mutants of cowpea mosaic virus.
Topics: Avidin; Binding Sites; Biotin; Capsid; Chromatography, Liquid; Comovirus; Cysteine; Electrophoresis, Polyacrylamide Gel; Fluorescein; Gold Colloid; Microscopy, Electron; Models, Molecular; Mutagenesis, Insertional; Nanotechnology; Protein Subunits; Reverse Transcriptase Polymerase Chain Reaction; Rhodamines; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds | 2002 |
A novel synthesis of (+)-biotin from L-cysteine.
Topics: Biotin; Cysteine; Indicators and Reagents; Models, Molecular; Molecular Conformation; Stereoisomerism | 2002 |
Disulfide bond switches.
Topics: Arsenicals; Biotin; CD4 Antigens; CD4-Positive T-Lymphocytes; Cysteine; Disulfides; Glutathione; HIV-1; Humans; Immunoblotting; Lysine; Maleimides; Mutation; Oxidation-Reduction; Protein Structure, Tertiary; Receptors, HIV; Succinimides; Sulfhydryl Compounds; Thioredoxins | 2002 |
Functional immobilization of a ligand-activated G-protein-coupled receptor.
Topics: Animals; Antibodies, Monoclonal; Avidin; Biotin; Cells, Cultured; Cysteine; Epitopes; Fluoresceins; GTP-Binding Proteins; Humans; Insecta; Kinetics; Ligands; Microscopy, Fluorescence; Protein Conformation; Receptors, Adrenergic, beta-2; Substrate Specificity; Surface Plasmon Resonance | 2002 |
Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces.
Topics: Animals; Avidin; Biotin; Calorimetry; Cell Line; Chickens; Cysteine; Disulfides; DNA, Complementary; Dose-Response Relationship, Drug; Insecta; Isoleucine; Models, Molecular; Mutation; Protein Binding; Protein Conformation; Protein Denaturation; Temperature; Time Factors | 2003 |
A ribozyme with michaelase activity: synthesis of the substrate precursors.
Topics: Amides; Biotin; Cysteine; Gene Library; Guanosine; Kinetics; Oligonucleotides; Phosphorus Isotopes; Photochemistry; Reverse Transcriptase Polymerase Chain Reaction; RNA, Catalytic; Substrate Specificity; Sulfhydryl Compounds; Templates, Genetic; Thymidylate Synthase | 2003 |
Diazotrifluoropropionamido-containing prenylcysteines: syntheses and applications for studying isoprenoid-protein interactions.
Topics: Amides; Autoradiography; Benzophenones; Binding Sites; Binding, Competitive; Biotin; Cross-Linking Reagents; Cysteine; Diazonium Compounds; Guanine Nucleotide Dissociation Inhibitors; Photoaffinity Labels; Photolysis; Protein Prenylation; rho-Specific Guanine Nucleotide Dissociation Inhibitors; Streptavidin | 2003 |
Proteomic analysis of S-nitrosylated proteins in mesangial cells.
Topics: Animals; Biotin; Cell Line; Cysteine; Electrophoresis, Gel, Two-Dimensional; Glomerular Mesangium; Mice; Nitric Oxide; Nitric Oxide Donors; Nitric Oxide Synthase Type II; Proteome; S-Nitrosoglutathione; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2003 |
Monitoring the cellular surface display of recombinant proteins by cysteine labeling and flow cytometry.
Topics: Adrenodoxin; Amino Acid Sequence; Animals; Bacterial Outer Membrane Proteins; Base Sequence; Biotin; Cattle; Cysteine; Escherichia coli; Escherichia coli Proteins; Flow Cytometry; Fluorescein; Maleimides; Models, Molecular; Molecular Sequence Data; Protein Transport; Recombinant Fusion Proteins; Staining and Labeling | 2003 |
Semisynthesis and characterization of the first analogues of pro-neuropeptide y.
Topics: Amino Acid Sequence; Biotin; Cysteine; Escherichia coli; Esters; Fluoresceins; Molecular Sequence Data; Neuropeptide Y; Peptide Fragments; Proline; Protein Precursors; Protein Splicing; Recombinant Fusion Proteins; Staining and Labeling; Sulfhydryl Compounds | 2003 |
Detection of cysteine S-nitrosylation and tyrosine 3-nitration in kidney proteins.
Topics: Animals; Biotin; Blotting, Western; Chromatography, High Pressure Liquid; Cysteine; Electrochemistry; In Vitro Techniques; Kidney; Mice; Nitric Oxide; Proteins; Rats; Tyrosine | 2003 |
The region between transmembrane domains 1 and 2 of the reduced folate carrier forms part of the substrate-binding pocket.
Topics: Alanine; Amino Acid Sequence; Animals; Bacterial Proteins; Biotin; Biotinylation; Carrier Proteins; Cell Membrane; Cricetinae; Cysteine; Cytosol; Folic Acid; Green Fluorescent Proteins; Kinetics; Luminescent Proteins; Membrane Transport Proteins; Methotrexate; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Recombinant Fusion Proteins; Reduced Folate Carrier Protein; RNA; Streptolysins | 2003 |
POLYSACCHARIDE STORAGE AND GROWTH EFFICIENCY IN RUMINOCOCCUS ALBUS.
Topics: 4-Aminobenzoic Acid; Animals; Bacteria; Benzoates; Biotin; Butyrates; Cellobiose; Culture Media; Cysteine; Disaccharides; Fatty Acids; Fermentation; Polysaccharides; Polysaccharides, Bacterial; Pyridoxine; Research; Rumen; Ruminococcus; Sulfides | 1963 |
STUDIES ON THE FORMATION OF BIOTIN FROM DESTHIOBIOTIN AND SULFATE IN SACCHAROMYCES CEREVISIAE. I. ON BIOTIN FORMATION FROM DESTHIOBIOTIN BY WASHED CELL SUSPENSION OF YEAST.
Topics: Biotin; Cysteine; Glucose; Glutathione; Homocysteine; Metabolism; Methionine; Pharmacology; Research; Saccharomyces; Saccharomyces cerevisiae; Sulfates; Sulfides; Sulfur Oxides; Yeast, Dried | 1964 |
STUDIES ON THE FORMATION OF BIOTIN FROM DESTHIOBIOTIN AND SULFATE IN SACCHAROMYCES CEREVISIAE. II. ON SULFUR SOURCES OF BIOTIN FORMATION BY WASHED CELL SUSPENSION OF YEAST.
Topics: Biotin; Choline; Culture Media; Cysteine; Cystine; Ethionine; Glucose; Glutathione; Homocysteine; Metabolism; Methionine; Research; Saccharomyces; Saccharomyces cerevisiae; Sulfates; Sulfur; Sulfur Oxides; Yeast, Dried | 1964 |
STUDIES ON THE FORMATION OF BIOTIN FROM DESTHIOBIOTIN AND SULFATE IN SACCHAROMYCES CEREVISIAE. 3. ISOTOPIC STUDIES ON BIOTIN FORMATION FROM DESTHIOBIOTIN AND S35-LABELLED SULFATE BY WASHED CELL SUSPENSION OF YEAST.
Topics: Biotin; Chromatography; Culture Media; Cysteine; Cystine; Glucose; Metabolism; Methionine; Neurospora; Research; Saccharomyces; Saccharomyces cerevisiae; Sulfates; Yeast, Dried | 1964 |
Procollagen VII self-assembly depends on site-specific interactions and is promoted by cleavage of the NC2 domain with procollagen C-proteinase.
Topics: Amino Acid Sequence; Animals; Binding Sites; Biotin; Bone Morphogenetic Protein 1; Bone Morphogenetic Proteins; Cloning, Molecular; Collagen Type VII; Colorimetry; Cysteine; Dimerization; Disulfides; Humans; Metalloendopeptidases; Mice; Microscopy, Electron; Models, Molecular; Molecular Sequence Data; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Deletion | 2003 |
Mislocalization of membrane proteins associated with multidrug resistance in cisplatin-resistant cancer cell lines.
Topics: Antineoplastic Agents; ATP Binding Cassette Transporter, Subfamily B, Member 1; Biotin; Carbon Radioisotopes; Carboplatin; Carrier Proteins; Cell Membrane; Cisplatin; Cysteine; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Endoplasmic Reticulum Chaperone BiP; Folate Receptors, GPI-Anchored; HeLa Cells; Humans; KB Cells; Lysosomes; Methionine; Methotrexate; Receptors, Cell Surface; Succinimides; Sulfur Radioisotopes; Triiodobenzoic Acids; Tritium | 2003 |
S-Nitrosation regulates the activation of endogenous procaspase-9 in HT-29 human colon carcinoma cells.
Topics: Apoptosis; Ascorbic Acid; Biotin; Blotting, Western; Caspase 3; Caspase 8; Caspase 9; Caspases; Cell Death; Colonic Neoplasms; Cysteine; DNA; Enzyme Activation; Enzyme Inhibitors; Enzyme-Linked Immunosorbent Assay; HT29 Cells; Humans; Interferon-gamma; Models, Biological; Nitric Oxide; Nitric Oxide Synthase; Nitrosation; Precipitin Tests; Time Factors; Tumor Necrosis Factor-alpha | 2004 |
A microdomain formed by the extracellular ends of the transmembrane domains promotes activation of the G protein-coupled alpha-factor receptor.
Topics: Amino Acid Sequence; Binding Sites; Biotin; Cell Division; Cysteine; Genes, Reporter; Ligands; Mesylates; Models, Molecular; Mutation; Phenotype; Pheromones; Protein Binding; Protein Structure, Secondary; Receptors, G-Protein-Coupled; Receptors, Mating Factor; Receptors, Peptide; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Signal Transduction; Transcription Factors | 2004 |
Site-specific immobilization of biotinylated proteins for protein microarray analysis.
Topics: Avidin; Base Sequence; Biotin; Cysteine; Genetic Vectors; Humans; Molecular Sequence Data; Molecular Structure; Protein Array Analysis; Proteins; Recombinant Fusion Proteins | 2004 |
Site-specific peptide immobilization strategies for the rapid detection of kinase activity on microarrays.
Topics: Antibodies; Avidin; Biotin; Cysteine; Esters; Fluorescent Dyes; Molecular Structure; Peptides; Polyethylene Glycols; Protein Array Analysis; Protein Kinases | 2004 |
Membrane topology of system xc- light subunit reveals a re-entrant loop with substrate-restricted accessibility.
Topics: Amino Acid Transport System y+; Amino Acid Transport Systems; Binding Sites; Biotin; Cell Membrane; Cysteine; HeLa Cells; Humans; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Subunits; Recombinant Proteins | 2004 |
Identification of oxidized mitochondrial proteins in alcohol-exposed human hepatoma cells and mouse liver.
Topics: Alcohol Drinking; Animals; Bacterial Proteins; Biotin; Carcinoma, Hepatocellular; Cysteine; Electrophoresis, Gel, Two-Dimensional; Ethanol; Humans; Liver; Mice; Mitochondria; Mitochondrial Proteins; Oxidation-Reduction; Sepharose; Sequence Analysis, Protein | 2004 |
A practical synthesis of (+)-biotin from L-cysteine.
Topics: Biotin; Cysteine; Molecular Structure; Vitamin B Complex | 2004 |
Electrospray mass spectrometry of a series of mixed nitrido 99gTc- heterocomplexes conjugated with bioactive molecules.
Topics: Biotin; Cysteine; Nitrogen Compounds; Piperazine; Piperazines; Radioisotopes; Spectrometry, Mass, Electrospray Ionization; Technetium; Technetium Compounds | 2004 |
Mutation of glutamate 155 of the GABAA receptor beta2 subunit produces a spontaneously open channel: a trigger for channel activation.
Topics: Animals; Binding Sites; Biotin; Cysteine; GABA-A Receptor Agonists; Glutamic Acid; Ion Channel Gating; Kinetics; Ligands; Mutagenesis, Site-Directed; Mutation; Patch-Clamp Techniques; Pentobarbital; Protein Conformation; Rats; Receptors, GABA-A; Recombinant Proteins; Xenopus laevis | 2004 |
Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits.
Topics: 3',5'-Cyclic-GMP Phosphodiesterases; Animals; Biotin; Blotting, Western; Catalysis; Catalytic Domain; Cattle; Cross-Linking Reagents; Cyclic Nucleotide Phosphodiesterases, Type 6; Cysteine; Dimerization; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Eye Proteins; Light; Mass Spectrometry; Models, Biological; Models, Chemical; Peptides; Phosphoric Diester Hydrolases; Protein Binding; Protein Structure, Tertiary; Retinal Rod Photoreceptor Cells; Serine; Ultraviolet Rays; Vision, Ocular | 2005 |
Selective detection of 2-deoxyribonolactone in DNA.
Topics: Autoradiography; Biotin; Cysteine; DNA; DNA Damage; Gas Chromatography-Mass Spectrometry; Horseradish Peroxidase; Oxidation-Reduction; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sugar Acids | 2005 |
Characterization and application of the biotin-switch assay for the identification of S-nitrosated proteins.
Topics: Animals; Ascorbic Acid; Biotin; Cells, Cultured; Cysteine; Mice; Nitrosation; Oxidation-Reduction; Proteins; Proteomics; S-Nitrosothiols | 2005 |
A pulsed potential waveform displaying enhanced detection capabilities towards sulfur-containing compounds at a gold working electrode.
Topics: Acetylcysteine; Biotin; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cysteine; Cystine; Electrochemistry; Electrodes; Glutathione; Gold; Homocysteine; Methionine; Oxidation-Reduction; Reproducibility of Results; Sensitivity and Specificity; Sulfur Compounds; Thioctic Acid | 2005 |
S-nitrosylation of Hsp90 promotes the inhibition of its ATPase and endothelial nitric oxide synthase regulatory activities.
Topics: Adenosine Triphosphatases; Amino Acid Sequence; Biotin; Cysteine; Endothelial Cells; HSP90 Heat-Shock Proteins; Humans; Luminescent Measurements; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Nitric Oxide Synthase; Nitric Oxide Synthase Type III; Ozone; Protein Processing, Post-Translational; S-Nitrosothiols; Signal Transduction | 2005 |
Specific patterns of electrophile adduction trigger Keap1 ubiquitination and Nrf2 activation.
Topics: Biotin; Cell Cycle Proteins; Cell Line; Cullin Proteins; Cysteine; DNA-Binding Proteins; Electrophoresis, Polyacrylamide Gel; Humans; Intracellular Signaling Peptides and Proteins; Kelch-Like ECH-Associated Protein 1; Mass Spectrometry; NF-E2-Related Factor 2; Peptides; Proteins; Trans-Activators; Transfection; Ubiquitin | 2005 |
Transforming activity of the Rho family GTPase, Wrch-1, a Wnt-regulated Cdc42 homolog, is dependent on a novel carboxyl-terminal palmitoylation motif.
Topics: Amino Acid Motifs; Amino Acid Sequence; Animals; Biotin; Blotting, Western; cdc42 GTP-Binding Protein; Cell Adhesion; Cell Membrane; Cell Proliferation; Cysteine; Cytosol; Endosomes; Esters; Green Fluorescent Proteins; Mice; Microscopy, Fluorescence; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; NIH 3T3 Cells; Palmitic Acid; Protein Binding; Protein Structure, Tertiary; Recombinant Proteins; rho GTP-Binding Proteins; Sequence Homology, Amino Acid; Signal Transduction; Transfection | 2005 |
An optimized strategy for ICAT quantification of membrane proteins.
Topics: Affinity Labels; Animals; Biotin; Cattle; Chickens; Cysteine; Electrophoresis, Gel, Two-Dimensional; Embryo, Mammalian; Embryo, Nonmammalian; Isotope Labeling; Lactoglobulins; Membrane Proteins; Mice; Ovalbumin; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stem Cells; Trypsin; Urea | 2006 |
Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2.
Topics: Animals; Biotin; Catalysis; Cell Line; Cyclooxygenase 2; Cysteine; Dinoprostone; Enzyme Activation; Humans; Mice; Nitric Oxide Donors; Nitric Oxide Synthase Type II; Nitroso Compounds; Protein Binding; S-Nitrosoglutathione | 2005 |
Cytosolic and nuclear protein targets of thiol-reactive electrophiles.
Topics: Alkylation; Amino Acid Sequence; Biotin; Cell Line; Cell-Free System; Cysteine; Cytosol; Humans; Nuclear Proteins; Peptide Fragments; Peptide Mapping; Protein Binding; Protein Conformation; Proteins; Proteomics; Sulfhydryl Compounds | 2006 |
Inhibition of protein phosphatase 2A activity by selective electrophile alkylation damage.
Topics: Alkylation; Biotin; Blotting, Western; Catalysis; Cell Line; Cysteine; Enzyme Activation; Enzyme Inhibitors; Holoenzymes; Humans; Phosphoprotein Phosphatases; Phosphorylation; Protein Conformation; Protein Phosphatase 2; Signal Transduction; Sulfhydryl Compounds | 2006 |
Plant viral capsids as nanobuilding blocks: construction of arrays on solid supports.
Topics: Biotin; Biotinylation; Capsid; Cysteine; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Gold; Models, Chemical; Molecular Conformation; Nanoparticles; Nanostructures; Nanotechnology; Plant Viruses; Protein Array Analysis; Spectrometry, Fluorescence | 2006 |
Design and characterization of an active site selective caspase-3 transnitrosating agent.
Topics: Apoptosis; Binding Sites; Biotin; Caspase 3; Caspase 9; Chemistry, Pharmaceutical; Cyclic GMP; Cysteine; Drug Design; Enzyme Activation; Humans; Kinetics; Nitric Oxide; Nitrosation; Peptides | 2006 |
Inorganic mercury interacts with cysteine residues (C451 and C474) of hOCT2 to reduce its transport activity.
Topics: Animals; Biological Transport; Biotin; CHO Cells; Cricetinae; Cricetulus; Cysteine; Humans; Kinetics; Mercuric Chloride; Organic Cation Transport Proteins; Organic Cation Transporter 2; Substrate Specificity; Tetraethylammonium | 2007 |
Assessment and application of the biotin switch technique for examining protein S-nitrosylation under conditions of pharmacologically induced oxidative stress.
Topics: Ascorbic Acid; Biotin; Cells, Cultured; Cysteine; Glutathione; Humans; Kidney; NG-Nitroarginine Methyl Ester; Nitric Oxide; Nitric Oxide Synthase; Nitrogen; Oxidation-Reduction; Oxidative Stress; Photolysis; Protein Tyrosine Phosphatase, Non-Receptor Type 1; Protein Tyrosine Phosphatases; Sulfhydryl Compounds; Sunlight | 2007 |
Evolution of an interloop disulfide bond in high-affinity antibody mimics based on fibronectin type III domain and selected by yeast surface display: molecular convergence with single-domain camelid and shark antibodies.
Topics: Amino Acid Sequence; Animals; Antibodies; Biomimetics; Biotin; Camelids, New World; Cysteine; Disulfides; Fibronectins; Humans; Models, Molecular; Molecular Sequence Data; Muramidase; Mutation; Peptide Library; Protein Structure, Tertiary; RNA, Messenger; Saccharomyces cerevisiae; Sharks | 2007 |
Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays.
Topics: Biotin; Cell-Free System; Cysteine; Protein Array Analysis; Proteins | 2006 |
Analysis of protein adduction kinetics by quantitative mass spectrometry: competing adduction reactions of glutathione-S-transferase P1-1 with electrophiles.
Topics: Biotin; Cysteine; Glutathione S-Transferase pi; Isocyanates; Kinetics; Naphthalenesulfonates; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry | 2007 |
Protein targets of reactive electrophiles in human liver microsomes.
Topics: Acetaminophen; Alkylating Agents; Biotin; Blotting, Western; Calreticulin; Cell Line; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Cysteine; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Glutathione Transferase; Humans; Iodoacetamide; Microsomes, Liver; Molecular Structure; Protein Binding; Proteins; Tandem Mass Spectrometry | 2007 |
Identification of arsenic-binding proteins in human breast cancer cells.
Topics: Antineoplastic Agents; Arsenic; Biotin; Breast Neoplasms; Carrier Proteins; Cell Line, Tumor; Cysteine; Humans; Models, Chemical; Models, Molecular; Molecular Conformation; Protein Binding; Pyruvate Kinase; Tubulin | 2007 |
Outward stabilization of the S4 segments in domains III and IV enhances lidocaine block of sodium channels.
Topics: Anesthetics, Local; Anti-Arrhythmia Agents; Arginine; Binding Sites; Biotin; Cell Line; Cysteine; Dose-Response Relationship, Drug; Humans; Ion Channel Gating; Kinetics; Lidocaine; Membrane Potentials; Muscle Proteins; Mutation; NAV1.5 Voltage-Gated Sodium Channel; Protein Conformation; Protein Structure, Tertiary; Sodium Channel Blockers; Sodium Channels; Transfection | 2007 |
Setting up for the block: the mechanism underlying lidocaine's use-dependent inhibition of sodium channels.
Topics: Anesthetics, Local; Anti-Arrhythmia Agents; Arginine; Binding Sites; Biotin; Cell Line; Cysteine; Humans; Ion Channel Gating; Kinetics; Lidocaine; Membrane Potentials; Muscle Proteins; Mutation; NAV1.5 Voltage-Gated Sodium Channel; Protein Conformation; Protein Structure, Tertiary; Sodium Channel Blockers; Sodium Channels; Transfection | 2007 |
Avidin-biotin system: a small library of cysteine biotinylated derivatives designed for the [99mTc(N)(PNP)]2+ metal fragment.
Topics: Animals; Avidin; Biotin; Biotinylation; Cysteine; Drug Design; Drug Stability; Humans; Male; Metabolic Clearance Rate; Metals; Mice; Mice, Inbred BALB C; Organ Specificity; Peptide Library; Radiopharmaceuticals; Rats; Technetium; Tissue Distribution | 2007 |
Unbiased identification of cysteine S-nitrosylation sites on proteins.
Topics: Animals; Ascorbic Acid; Biotin; Cysteine; Mice; Nerve Tissue Proteins; Nitric Oxide; Nitric Oxide Synthase; Nitroso Compounds; Proteome; Proteomics; Trypsin | 2007 |
The natural product avrainvillamide binds to the oncoprotein nucleophosmin.
Topics: Alanine; Animals; Antineoplastic Agents; Binding Sites; Biological Products; Biotin; Blotting, Western; Chlorocebus aethiops; COS Cells; Cysteine; HeLa Cells; Humans; Indoles; Microscopy, Fluorescence; Nuclear Proteins; Nucleophosmin; Oncogene Proteins; Tumor Cells, Cultured | 2007 |
Sites of alkylation of human Keap1 by natural chemoprevention agents.
Topics: Alkylation; Amino Acid Sequence; Biological Products; Biotin; Chemoprevention; Chromatography, Liquid; Cysteine; Humans; Intracellular Signaling Peptides and Proteins; Kelch-Like ECH-Associated Protein 1; Mass Spectrometry; Molecular Sequence Data; Peptides; Protein Processing, Post-Translational | 2007 |
Anti-parallel membrane topology of a homo-dimeric multidrug transporter, EmrE.
Topics: Antiporters; Biological Transport; Biotin; Cell Membrane Permeability; Cysteine; Cytoplasm; Dimerization; Escherichia coli Proteins; Maleimides; Membrane Transport Proteins; Multidrug Resistance-Associated Proteins; Mutation; Periplasm; Polyethylene; Proline; Protein Structure, Secondary; Threonine | 2007 |
Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins.
Topics: Biochemistry; Biotin; Bronchi; Carbocyanines; Copper; Cysteine; Epithelial Cells; Free Radicals; Hemoglobins; Humans; Models, Biological; Nitric Oxide; Nitrogen; S-Nitrosothiols; Sulfhydryl Compounds | 2008 |
Rapid identification of reactive cysteine residues for site-specific labeling of antibody-Fabs.
Topics: Antibodies, Monoclonal; Antigen-Antibody Reactions; Binding Sites; Biotin; Cell Line, Tumor; Chromatography, Liquid; Cysteine; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Gene Expression Regulation; Genetic Engineering; Genetic Variation; Humans; Immunoglobulin Fab Fragments; Maleimides; Mass Spectrometry; Models, Molecular; Mutagenesis, Site-Directed; Plasmids; Sensitivity and Specificity; Staining and Labeling; Surface Properties | 2008 |
On-gel fluorescent visualization and the site identification of S-nitrosylated proteins.
Topics: Acetic Acid; Animals; Binding Sites; Biotin; Cattle; Chromatography, Liquid; Cysteine; Fluorescence; Gels; Nitric Oxide; Proteins; Serum Albumin, Bovine; Staining and Labeling; Tandem Mass Spectrometry; Time Factors | 2008 |
Mechanism for the cholesterol-lowering action of egg white protein in rats.
Topics: Animals; Biotin; Cholesterol; Cysteine; Egg Proteins; Feces; Intestinal Mucosa; Intestines; Lipid Metabolism; Liver; Male; Micelles; Rats; Rats, Sprague-Dawley; Solid Phase Extraction | 2008 |
Probing conformational changes of prestin with thiol-reactive optical switches.
Topics: Animals; Anion Transport Proteins; Biotin; Cell Membrane; Cysteine; Electric Capacitance; Guinea Pigs; Hair Cells, Auditory, Outer; Humans; Iodoacetamide; Maleimides; Optics and Photonics; Protein Conformation; Sulfhydryl Compounds; Transfection | 2008 |
Targeting of adenovirus vectors to the LRP receptor family with the high-affinity ligand RAP via combined genetic and chemical modification of the pIX capsomere.
Topics: Adenoviridae; Animals; Biotin; Capsid Proteins; Cell Line; Cysteine; Female; Genetic Vectors; Humans; LDL-Receptor Related Protein-Associated Protein; LDL-Receptor Related Proteins; Ligands; Maleimides; Mice; Mice, Inbred BALB C; Receptors, Transferrin; Transferrin; Virion | 2008 |
Alteration of S-nitrosothiol homeostasis and targets for protein S-nitrosation in human hepatocytes.
Topics: Adult; Aged; Aldehyde Oxidoreductases; Analysis of Variance; Biotin; Chromatography, Liquid; Cysteine; Female; Hepatocytes; Homeostasis; Humans; Liver Diseases; Male; Middle Aged; Nitric Oxide; Nitrosation; Phosphatidylethanolamine Binding Protein; Protein Disulfide-Isomerases; Proteins; S-Nitrosothiols; Tandem Mass Spectrometry; Tumor Cells, Cultured | 2008 |
Two highly conserved cysteine residues in HPV16 L2 form an intramolecular disulfide bond and are critical for infectivity in human keratinocytes.
Topics: Amino Acid Sequence; Animals; Biotin; Capsid Proteins; Cell Line; Cysteine; Disulfides; Human papillomavirus 16; Humans; Keratinocytes; Molecular Sequence Data; Oncogene Proteins, Viral; Oxidation-Reduction; Sequence Alignment; Virion | 2009 |
Analysis of oxidative modification of proteins.
Topics: Biochemistry; Biotin; Borohydrides; Cysteine; Isoaspartic Acid; Isotope Labeling; Oxidation-Reduction; Phenylhydrazines; Protein Binding; Proteins; Sulfhydryl Compounds; Tyrosine | 2009 |
Synthesis and application of a N-1' fluorescent biotinyl derivative inducing the specific carboxy-terminal dual labeling of a novel RhoB-selective scFv.
Topics: Antibody Specificity; Binding Sites; Biotin; Cysteine; Esters; Fluorescent Antibody Technique; Fluorescent Dyes; HeLa Cells; Humans; Inteins; Nitrogen; Protein Splicing; rhoB GTP-Binding Protein; Single-Chain Antibodies; Staining and Labeling; Substrate Specificity | 2009 |
Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.
Topics: Biotin; Cell Line; Cysteine; Glyceraldehyde-3-Phosphate Dehydrogenases; Humans; Mass Spectrometry; Proteome; Proteomics; S-Nitrosothiols; Sensitivity and Specificity; Sulfhydryl Compounds | 2009 |
Use of intein-mediated protein ligation strategies for the fabrication of functional protein arrays.
Topics: Animals; Avidin; Biotin; Biotinylation; Cysteine; Gene Expression; Green Fluorescent Proteins; Humans; Immobilized Proteins; Inteins; Peptides; Protein Array Analysis; Protein Splicing; Protein Structure, Tertiary; Proteins; Puromycin; Recombinant Fusion Proteins; Sulfhydryl Reagents | 2009 |
Novel twist on the biotin switch cracks endothelial cell S-nitrosoproteome.
Topics: Biotin; Cysteine; Penicillamine; Proteins; Proteomics; S-Nitroso-N-Acetylpenicillamine; S-Nitrosothiols | 2009 |
S-Nitrosylation of DRP1 does not affect enzymatic activity and is not specific to Alzheimer's disease.
Topics: Aging; Alzheimer Disease; Biotin; Brain; Cell Line, Transformed; Cysteine; Dithiothreitol; Dynamins; GTP Phosphohydrolases; Humans; Microscopy, Electron, Transmission; Microtubule-Associated Proteins; Mitochondrial Diseases; Mitochondrial Proteins; Postmortem Changes; Protein Multimerization | 2010 |
Epitope mapping in cell surface proteins by site-directed masking: defining the structural elements of NTPDase3 inhibition by a monoclonal antibody.
Topics: Adenosine Triphosphatases; Animals; Antibodies, Monoclonal; Binding Sites, Antibody; Biotin; Chlorocebus aethiops; COS Cells; Cysteine; Enzymes, Immobilized; Epitope Mapping; Epitopes; Humans; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Rats | 2010 |
Proteomic and mass spectroscopic quantitation of protein S-nitrosation differentiates NO-donors.
Topics: Biotin; Cells, Cultured; Cysteine; Glutathione S-Transferase pi; Humans; Mass Spectrometry; Neuroblastoma; Nitric Oxide; Nitric Oxide Donors; Nitrosation; Proteins; Proteomics; S-Nitrosoglutathione; S-Nitrosothiols | 2010 |
Selective covalent labeling of tag-fused GPCR proteins on live cell surface with a synthetic probe for their functional analysis.
Topics: Biotin; Cell Line; Cysteine; Endocytosis; Endosomes; Fluoresceins; Humans; Molecular Probe Techniques; Molecular Probes; Oligopeptides; Organometallic Compounds; Receptors, G-Protein-Coupled; Staining and Labeling | 2010 |
Acyl-biotinyl exchange chemistry and mass spectrometry-based analysis of palmitoylation sites of in vitro palmitoylated rat brain tubulin.
Topics: Amino Acid Sequence; Animals; Bacterial Proteins; Binding Sites; Biotin; Brain; Cysteine; Lipoylation; Mass Spectrometry; Molecular Sequence Data; Palmitic Acid; Protein Engineering; Proteomics; Rats; Sequence Analysis; Signal Transduction; Tubulin | 2010 |
S-nitrosylation of cyclin-dependent kinase 5 (cdk5) regulates its kinase activity and dendrite growth during neuronal development.
Topics: Animals; Biotin; Brain Chemistry; Cells, Cultured; Cyclin-Dependent Kinase 5; Cysteine; Dendrites; DNA, Complementary; Hippocampus; Humans; Mice; Neurons; Nitroso Compounds; Protein Processing, Post-Translational; Recombinant Fusion Proteins; Transfection | 2010 |
Nitrosative protein oxidation is modulated during early endotoxemia.
Topics: Animals; Biotin; Blood Pressure; Cysteine; Endotoxemia; Escherichia coli; Heart; HEK293 Cells; Humans; Hypotension; Immunoblotting; In Vitro Techniques; Lipopolysaccharides; Mass Spectrometry; Metalloporphyrins; Mice; Mice, Inbred C57BL; Models, Animal; Nitric Oxide; Nitric Oxide Synthase; Oxidation-Reduction; Peroxynitrous Acid; Protein Processing, Post-Translational; Proteins; Rats; Rats, Wistar; S-Nitroso-N-Acetylpenicillamine; Sensitivity and Specificity; Sepsis; Signal Transduction; Telemetry; Tyrosine | 2011 |
In vitro selection of proteins that undergo covalent labeling with small molecules by thiol-disulfide exchange by using ribosome display.
Topics: Amino Acid Sequence; Amino Acid Substitution; Biotin; Cysteine; Disulfides; Methionine; Molecular Sequence Data; Mutant Proteins; Mutation; Peptides; Protein Engineering; Protein Structure, Tertiary; Ribosomes; Sulfhydryl Compounds; Sulfur Radioisotopes | 2011 |
Purification of tetracysteine-tagged proteins by affinity chromatography using a non-fluorescent, photochemically stable bisarsenical affinity ligand.
Topics: Arsenicals; Biotin; Chromatography, Affinity; Cysteine; Kinetics; Ligands; Molecular Structure; Photochemical Processes; Proteins; Stereoisomerism | 2011 |
Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates.
Topics: Avidin; Biocompatible Materials; Biotin; Chromatography, Affinity; Cloning, Molecular; Cross-Linking Reagents; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gels; Immobilized Proteins; Ink; Phase Transition; Plasmids; Polymerase Chain Reaction; Polymers; Propylamines; Protein Binding; Protein Engineering; Recombinant Proteins; Silanes; Succinimides; Surface Properties | 2011 |
Agonist- and hydrogen peroxide-mediated oxidation of the β2 adrenergic receptor: evidence of receptor s-sulfenation as detected by a modified biotin-switch assay.
Topics: Adrenergic beta-2 Receptor Agonists; Alkylation; Biological Assay; Biotin; Cyclohexanones; Cysteine; Dose-Response Relationship, Drug; Epithelial Cells; Ethanolamines; Formoterol Fumarate; HEK293 Cells; Humans; Hydrogen Peroxide; Isoproterenol; Oxidation-Reduction; Pulmonary Alveoli; Reactive Oxygen Species; Receptors, Adrenergic, beta-2; Signal Transduction; Sulfenic Acids; Time Factors | 2011 |
An affinity purification procedure to isolate oxidized p53.
Topics: Avidin; Biotin; Chromatography, Affinity; Cysteine; Humans; Oxidation-Reduction; Sulfhydryl Compounds; Tumor Suppressor Protein p53 | 2012 |
N-biotinyl-S-(1,2-dichlorovinyl)-L-cysteine sulfoxide as a potential model for S-(1,2-dichlorovinyl)-L-cysteine sulfoxide: characterization of stability and reactivity with glutathione and kidney proteins in vitro.
Topics: Animals; Binding Sites; Binding, Competitive; Biotin; Biotinylation; Blotting, Western; Cysteine; Dose-Response Relationship, Drug; Drug Stability; Electrophoresis, Polyacrylamide Gel; Glutathione Reductase; Half-Life; Humans; Indicators and Reagents; Kidney; Kidney Neoplasms; Malate Dehydrogenase; Protein Binding; Rats, Sprague-Dawley | 2011 |
Cys(294) is essential for the function of the human sodium-dependent multivitamin transporter.
Topics: Biological Transport; Biotin; Biotinylation; Cell Membrane; Cysteine; DNA Primers; Epithelial Cells; Humans; Kinetics; Mutagenesis, Site-Directed; Mutation; Polymerase Chain Reaction; Retina; Structure-Activity Relationship; Symporters; Transfection | 2012 |
DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools.
Topics: Amino Acid Sequence; Animals; Avidin; Biotin; Calorimetry, Differential Scanning; Chickens; Cysteine; Directed Molecular Evolution; DNA Shuffling; Molecular Sequence Data; Mutation; Protein Stability; Recombinant Fusion Proteins; Sequence Alignment | 2012 |
Identification and quantification of S-nitrosylation by cysteine reactive tandem mass tag switch assay.
Topics: Biotin; Blotting, Western; Cells, Cultured; Cysteine; Endothelium, Vascular; Humans; Nitric Oxide; Oxidation-Reduction; Protein Processing, Post-Translational; Proteomics; Pulmonary Artery; S-Nitrosoglutathione; Sulfhydryl Compounds; Tandem Mass Spectrometry | 2012 |
Site-specific immobilization of enzymes on magnetic nanoparticles and their use in organic synthesis.
Topics: Binding Sites; Biotin; Chemistry Techniques, Synthetic; Cysteine; Enzymes, Immobilized; Kinetics; Magnetite Nanoparticles; Neisseria gonorrhoeae; Oxo-Acid-Lyases; Pasteurella multocida; Polyethylene Glycols; Sialyltransferases; Streptavidin; Substrate Specificity | 2012 |
Purification of histidine-tagged aequorin with a reactive cysteine residue for chemical conjugations and its application for bioluminescent sandwich immunoassays.
Topics: Aequorin; alpha-Fetoproteins; Biotin; Cysteine; Histidine; Immunoassay; Luminescent Measurements; Maleimides; Models, Chemical; Recombinant Fusion Proteins; Sensitivity and Specificity; Streptavidin | 2012 |
Improving the selectivity of the phosphoric acid β-elimination on a biotinylated phosphopeptide.
Topics: Acetonitriles; Biotin; Cysteine; Hydrogen Sulfide; Limit of Detection; Phosphopeptides; Phosphoric Acids; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature; Water | 2012 |
S-nitrosylated protein disulfide isomerase contributes to mutant SOD1 aggregates in amyotrophic lateral sclerosis.
Topics: Amyotrophic Lateral Sclerosis; Animals; Arginine; Biotin; Cell Line, Tumor; Cysteine; Disease Models, Animal; Disease Progression; Humans; Male; Mice; Mice, Transgenic; Mutation; Neuroblastoma; Nitric Oxide; Nitric Oxide Synthase; Nitric Oxide Synthase Type II; Protein Disulfide-Isomerases; Protein Folding; S-Nitrosothiols; Spinal Cord; Subcellular Fractions; Superoxide Dismutase; Up-Regulation | 2013 |
Chasing cysteine oxidative modifications: proteomic tools for characterizing cysteine redox status.
Topics: Acylation; Biotin; Chromatography, High Pressure Liquid; Cysteine; Glutathione; Humans; Oxidation-Reduction; Proteomics; Reactive Oxygen Species; Sulfhydryl Compounds; Sulfinic Acids; Sulfonic Acids; Tandem Mass Spectrometry | 2012 |
Long range effect of mutations on specific conformational changes in the extracellular loop 2 of angiotensin II type 1 receptor.
Topics: Animals; Biotin; Calcium; Chlorocebus aethiops; COS Cells; Cysteine; Ligands; Molecular Conformation; Molecular Dynamics Simulation; Mutagenesis; Mutation; Protein Conformation; Protein Structure, Tertiary; Rats; Receptor, Angiotensin, Type 1; Receptors, G-Protein-Coupled; Signal Transduction | 2013 |
Rocket fuel for the quantification of S-nitrosothiols. Highly specific reduction of S-nitrosothiols to thiols by methylhydrazine.
Topics: Ascorbic Acid; Biotin; Cysteine; Glutathione; Magnetic Resonance Spectroscopy; Methylhydrazines; Nitric Oxide; Nitrosation; Oxidation-Reduction; Proteins; Reactive Nitrogen Species; Reactive Oxygen Species; S-Nitrosothiols; Sulfhydryl Compounds | 2013 |
Triple cysteine module within M-type K+ channels mediates reciprocal channel modulation by nitric oxide and reactive oxygen species.
Topics: Action Potentials; Aniline Compounds; Animals; Animals, Newborn; Anthracenes; Benzoates; Biotin; Calcitonin Gene-Related Peptide; Carbamates; Cells, Cultured; Cysteine; Enzyme Inhibitors; Female; Humans; Imidazoles; KCNQ Potassium Channels; Male; Membrane Transport Modulators; Models, Molecular; Mutation; Neurons; Nitric Oxide; Nitric Oxide Donors; Patch-Clamp Techniques; Phenylenediamines; Potassium Channel Blockers; Rats; Reactive Oxygen Species; S-Nitroso-N-Acetylpenicillamine; Transfection; Trigeminal Ganglion | 2013 |
Quantification of mitochondrial S-nitrosylation by CysTMT⁶ switch assay.
Topics: Animals; Biological Assay; Biotin; Cysteine; Mitochondria, Heart; Mitochondrial Proteins; Myocardium; Nitric Oxide; Oxidation-Reduction; Protein Processing, Post-Translational; Rats; S-Nitrosoglutathione | 2013 |
Effect of length of molecular recognition moiety on enzymatic activity switching.
Topics: Avidin; Biotin; Camphor 5-Monooxygenase; Catalytic Domain; Cysteine; Mutagenesis, Site-Directed; Polyethylene Glycols | 2013 |
Convenient method to assess chemical modification of protein thiols by electrophilic metals.
Topics: Adaptor Proteins, Signal Transducing; Animals; Biotin; Cysteine; Cytoskeletal Proteins; Electrophoresis, Polyacrylamide Gel; Hepatocytes; Humans; Kelch-Like ECH-Associated Protein 1; Male; Maleimides; Methylmercury Compounds; Mice; Mice, Inbred C57BL; Neuroblastoma; Sulfhydryl Compounds; Tumor Cells, Cultured | 2013 |
Solid-phase synthesis of Biotin-S-Farnesyl-L-Cysteine, a surrogate substrate for isoprenylcysteine Carboxylmethyltransferase (ICMT).
Topics: Biotin; Cysteine; High-Throughput Screening Assays; Kinetics; Protein Methyltransferases; Solid-Phase Synthesis Techniques; Substrate Specificity; Trityl Compounds | 2013 |
Amplified electrochemiluminescence of luminol based on hybridization chain reaction and in situ generate co-reactant for highly sensitive immunoassay.
Topics: Animals; Antibodies, Anti-Idiotypic; Biotin; Biotinylation; Cysteine; DNA, Single-Stranded; Electrodes; Enzymes, Immobilized; Glucose; Glucose Oxidase; Goats; Graphite; Humans; Hydrogen Peroxide; Immunoassay; Immunoglobulin G; Limit of Detection; Luminescent Measurements; Luminol; Mice; Oxides; Streptavidin | 2013 |
Selective conjugation of proteins by mining active proteomes through click-functionalized magnetic nanoparticles.
Topics: Azides; Biotin; Click Chemistry; Cysteine; Data Mining; Ferric Compounds; Leucine; Luminescence; Magnetics; Microwaves; Nanoparticles; Nanotechnology; Peptide Hydrolases; Plant Proteins; Proteome; Rhodamines; Spectroscopy, Fourier Transform Infrared; Streptavidin; Thermogravimetry | 2013 |
Differential S-nitrosylation of proteins in Alzheimer's disease.
Topics: Aged; Alzheimer Disease; Biotin; Brain; Case-Control Studies; Cysteine; Electrophoresis, Gel, Two-Dimensional; Female; Fructose-Bisphosphate Aldolase; Humans; Male; Proteomics; S-Nitrosothiols; Superoxide Dismutase; Tandem Mass Spectrometry; Voltage-Dependent Anion Channel 2 | 2014 |
Detection of protein S-sulfhydration by a tag-switch technique.
Topics: Biotin; Cysteine; Glutathione Peroxidase; Human Umbilical Vein Endothelial Cells; Humans; Hydrogen Sulfide; Jurkat Cells; Microscopy, Fluorescence; Protein Processing, Post-Translational; Serum Albumin, Bovine; Sulfhydryl Compounds; Sulfides | 2014 |
S-nitrosylation influences the structure and DNA binding activity of AtMYB30 transcription factor from Arabidopsis thaliana.
Topics: Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Binding Sites; Biological Assay; Biotin; Circular Dichroism; Cysteine; DNA, Plant; Escherichia coli; Molecular Sequence Data; Mutagenesis, Site-Directed; Nitric Oxide; Nitric Oxide Donors; Protein Binding; Protein Structure, Secondary; Recombinant Proteins; Sequence Alignment; Transcription Factors | 2014 |
Oxygen-linked S-nitrosation in fish myoglobins: a cysteine-specific tertiary allosteric effect.
Topics: Allosteric Regulation; Amino Acid Sequence; Animals; Biotin; Cysteine; Fish Proteins; Fishes; Heme; Humans; Kinetics; Molecular Sequence Data; Myocardium; Myoglobin; Nitric Oxide; Nitrosation; Oxygen; Species Specificity | 2014 |
Sulfhydryl-specific probe for monitoring protein redox sensitivity.
Topics: Amino Acid Sequence; Biotin; Biotinylation; Cysteine; HeLa Cells; Humans; Hydrogen Peroxide; Molecular Probes; Molecular Sequence Data; Nitrobenzoates; Oxidation-Reduction; Oxidative Stress; Proteins; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining and Labeling; Streptavidin; Sulfhydryl Compounds; Sulfonamides | 2014 |
Endogenous S-sulfhydration of PTEN helps protect against modification by nitric oxide.
Topics: Biotin; Cell Line, Tumor; Cystathionine beta-Synthase; Cysteine; Humans; Hydrogen Sulfide; Mutagenesis, Site-Directed; Neuroblastoma; Neurons; Nitric Oxide; Oxidative Stress; Plasmids; Proto-Oncogene Proteins c-akt; PTEN Phosphohydrolase; RNA, Small Interfering; Signal Transduction; Sulfur; Transfection | 2015 |
Use of the "tag-switch" method for the detection of protein S-sulfhydration.
Topics: Acetates; Benzothiazoles; Biotin; Cysteine; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); Human Umbilical Vein Endothelial Cells; Humans; Hydrogen Sulfide; Jurkat Cells; Microscopy, Fluorescence; Oxidation-Reduction; Protein Processing, Post-Translational; Serum Albumin, Bovine; Signal Transduction; Spectrometry, Mass, Electrospray Ionization; Staining and Labeling; Streptavidin; Sulfides | 2015 |
Protein sulfhydration.
Topics: Biotin; Cell Line; Cysteine; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); Humans; Hydrogen Sulfide; Maleimides; Morpholines; Organothiophosphorus Compounds; Oxidation-Reduction; Phenanthrolines; Protein Processing, Post-Translational; Signal Transduction; Staining and Labeling; Streptavidin; Sulfides | 2015 |
Identification of total reversible cysteine oxidation in an atherosclerosis model using a modified biotin switch assay.
Topics: Amino Acid Sequence; Atherosclerosis; Biological Assay; Biotin; Blood Platelets; Cell Extracts; Cell Line; Chromatography, Liquid; Cysteine; Humans; Metabolic Networks and Pathways; Models, Biological; Molecular Sequence Annotation; Molecular Sequence Data; Monocytes; NADPH Oxidases; Oxidation-Reduction; Peptides; Primary Cell Culture; Proteome; Reactive Oxygen Species; Tandem Mass Spectrometry | 2015 |
S-sulfhydration: a cysteine posttranslational modification in plant systems.
Topics: Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Ascorbate Peroxidases; Biotin; Cysteine; Cytosol; Immunoblotting; Mass Spectrometry; Molecular Sequence Data; Protein Processing, Post-Translational; Recombinant Proteins; Sulfides | 2015 |
Synthetic methodology for asymmetric ferrocene derived bio-conjugate systems via solid phase resin-based methodology.
Topics: Biotin; Cysteine; Ferrous Compounds; Gold; Metallocenes; Plastics; Solid-Phase Synthesis Techniques; Surface Properties | 2015 |
ATP Binding and Hydrolysis Properties of ABCB10 and Their Regulation by Glutathione.
Topics: Adenosine Triphosphate; Aminolevulinic Acid; Animals; ATP-Binding Cassette Transporters; Azides; Biotin; Conserved Sequence; Cysteine; Glutathione; Glutathione Disulfide; HEK293 Cells; Humans; Hydrolysis; Mice; Mitochondria; Mutation; Protein Structure, Tertiary; Submitochondrial Particles | 2015 |
Dual Labeling Biotin Switch Assay to Reduce Bias Derived From Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection.
Topics: Alcohol Dehydrogenase; Animals; Biotin; Cysteine; Female; HEK293 Cells; Humans; Male; Mice, Inbred C57BL; Mice, Knockout; Molecular Probes; Myocardium; Nitrosation; Nitroso Compounds; Protein Processing, Post-Translational; Proteomics; Reproducibility of Results; Tandem Mass Spectrometry | 2015 |
Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling.
Topics: Amino Acid Sequence; Biotin; Biotinylation; Carrier Proteins; Chromatography, Affinity; Cysteine; Electrophoresis, Polyacrylamide Gel; Kinetics; Ligands; Maltose-Binding Proteins; Models, Molecular; Molecular Sequence Data; Peptides; Protein Binding; Protein Engineering; Recombinant Fusion Proteins; Streptavidin; Sulfhydryl Compounds | 2015 |
The Cysteine-rich Domain of the DHHC3 Palmitoyltransferase Is Palmitoylated and Contains Tightly Bound Zinc.
Topics: Amino Acid Motifs; Animals; Biotin; Catalytic Domain; Chelating Agents; Cysteine; Iodoacetamide; Ions; Lipoylation; Lysine; Mass Spectrometry; Membrane Proteins; Metals; Mice; Mutation; Protein Binding; Protein Processing, Post-Translational; Sf9 Cells; Spodoptera; Zinc | 2015 |
SNOs Differ: Methodological and Biological Implications.
Topics: Animals; Biotin; Cysteine; Female; Humans; Male; Molecular Probes; Myocardium; Nitroso Compounds; Proteomics | 2015 |
The "π-Clamp" Offers a New Strategy for Site-Selective Protein Modification.
Topics: Biotin; Catalysis; Cysteine; Fluorocarbons; Glutathione Transferase; Protein Processing, Post-Translational; Proteins | 2016 |
Cloning, expression and characterization of histidine-tagged biotin synthase of Mycobacterium tuberculosis.
Topics: Antitubercular Agents; Bacterial Proteins; Biotin; Cloning, Molecular; Cysteine; Deoxyadenosines; Enzyme Inhibitors; Enzyme Stability; Histidine; Hydrogen-Ion Concentration; Kinetics; Microbial Sensitivity Tests; Mycobacterium tuberculosis; Protein Engineering; Protein Multimerization; Recombinant Fusion Proteins; S-Adenosylhomocysteine; S-Adenosylmethionine; Substrate Specificity; Sulfurtransferases; Temperature | 2016 |
[Reactive sulfur species-modified protein thiols: new methods for polysulfurated protein analysis].
Topics: Biotin; Cyanates; Cysteine; Disulfides; Humans; Proteins; Proteomics; Reagent Kits, Diagnostic; Sulfhydryl Compounds; Sulfides; Sulfur; Tumor Cells, Cultured | 2016 |
Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells.
Topics: Biotin; Blotting, Western; Cells, Cultured; Connexin 43; Cysteine; Fluorescent Antibody Technique; Human Umbilical Vein Endothelial Cells; Humans; Phosphorylation; Primary Cell Culture; Protein Processing, Post-Translational; S-Nitrosothiols; Serine; Threonine; Tyrosine | 2016 |
Biotin Switch Assays for Quantitation of Reversible Cysteine Oxidation.
Topics: Biological Assay; Biotin; Cysteine; Oxidation-Reduction | 2017 |
Development of a Small Hybrid Molecule That Mediates Degradation of His-Tag Fused Proteins.
Topics: Biotin; Cell Line; Cysteine; Histidine; Humans; Leupeptins; Maleimides; Nitrilotriacetic Acid; Organometallic Compounds; Proteasome Inhibitors; Proteolysis; Receptors, Retinoic Acid; Recombinant Fusion Proteins; Smad2 Protein; Ubiquitin-Protein Ligases | 2018 |
Chemical addressability of potato virus X for its applications in bio/nanotechnology.
Topics: Biotechnology; Biotin; Biotinylation; Contrast Media; Cysteine; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Heterocyclic Compounds; Immunohistochemistry; Magnetic Resonance Imaging; Microscopy, Electron, Transmission; Nanoparticles; Nanotechnology; Organometallic Compounds; Potexvirus; Tandem Mass Spectrometry | 2017 |
Release of Enzymatically Active Deubiquitinating Enzymes upon Reversible Capture by Disulfide Ubiquitin Reagents.
Topics: Biotin; Catalytic Domain; Cysteine; Deubiquitinating Enzymes; Disulfides; HeLa Cells; Humans; Polyethylene Glycols; Proteins; Ubiquitin; Ubiquitination | 2017 |
Native-MS Analysis of Monoclonal Antibody Conjugates by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
Topics: Antibodies, Monoclonal; Biotin; Cysteine; Immunoconjugates; Immunoglobulin G; Lysine; Mass Spectrometry | 2018 |
Monodisperse measurement of the biotin-streptavidin interaction strength in a well-defined pulling geometry.
Topics: Biotin; Calorimetry; Cysteine; Electrophoresis, Polyacrylamide Gel; Microscopy, Atomic Force; Streptavidin | 2017 |
Artificial Cysteine S-Glycosylation Induced by Per-O-Acetylated Unnatural Monosaccharides during Metabolic Glycan Labeling.
Topics: Acetylation; Alkynes; Azides; Biotin; Cysteine; Glutathione; Glycosylation; HeLa Cells; Humans; Metabolic Engineering; Monosaccharides; Peptides; Tandem Mass Spectrometry | 2018 |
Identification of S-Nitrosylated and Reversibly Oxidized Proteins by Fluorescence Switch and Complementary Techniques.
Topics: Biotin; Cysteine; Fluorescence; Mass Spectrometry; Nitric Oxide; Oxidation-Reduction; Protein Processing, Post-Translational; Proteomics; Staining and Labeling | 2018 |
Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Technique.
Topics: Biotin; Cysteine; Nitric Oxide; Nitrosation; Oxidation-Reduction; Protein Conformation; Protein Processing, Post-Translational; Recombinant Proteins | 2018 |
Identification of NO-Sensitive Cysteine Residues Using Cysteine Mutants of Recombinant Proteins.
Topics: Arabidopsis; Arabidopsis Proteins; Biotin; Chromatography, Affinity; Cloning, Molecular; Cysteine; Gene Expression; Mutagenesis, Site-Directed; Mutation; Nitric Oxide; Nitrosation; Recombinant Proteins | 2018 |
Site-Specific Protein Labeling with N-Hydroxysuccinimide-Esters and the Analysis of Ubiquitin Ligase Mechanisms.
Topics: Animals; Biotin; Cysteine; Escherichia coli; Esters; Fluoresceins; Fluorescent Dyes; Glutathione Transferase; Humans; Protein Binding; PTEN Phosphohydrolase; Rhodamines; Saccharomyces cerevisiae; Spodoptera; Succinimides; Ubiquitin; Ubiquitin-Protein Ligases; Ubiquitination; Uracil-DNA Glycosidase | 2018 |
Identification of S-nitrosylated proteins in postmortem pork muscle using modified biotin switch method coupled with isobaric tags.
Topics: Animals; Biotin; Chromatography, Liquid; Cysteine; Dietary Proteins; Food Analysis; Humans; Muscle Proteins; Muscles; Nitric Oxide; Nitroso Compounds; Peptides; Postmortem Changes; Proteolysis; Red Meat; S-Nitrosoglutathione; Sulfhydryl Compounds; Swine; Tandem Mass Spectrometry | 2018 |
Building and Breaking Bonds via a Compact S-Propargyl-Cysteine to Chemically Control Enzymes and Modify Proteins.
Topics: 3C Viral Proteases; Archaeal Proteins; Biotin; Catalysis; Catalytic Domain; Click Chemistry; Cysteine; Cysteine Endopeptidases; Enterovirus; Green Fluorescent Proteins; Humans; Methanosarcina; Mutagenesis, Site-Directed; Palladium; Pargyline; Thioredoxins; Viral Proteins | 2018 |
High-Affinity Antibody Detection with a Bivalent Circularized Peptide Containing Antibody-Binding Domains.
Topics: Amino Acid Sequence; Antibody Affinity; Bacterial Proteins; Binding Sites; Binding Sites, Antibody; Biotin; Chromatography, Affinity; Chromatography, Ion Exchange; Cysteine; Enzyme-Linked Immunosorbent Assay; Fungal Proteins; Humans; Immobilization; Immobilized Proteins; Immunoglobulin Fab Fragments; Immunoglobulin G; Kinetics; Models, Molecular; Molecular Probe Techniques; Peptide Hydrolases; Peptides; Protein Binding; Protein Domains; Substrate Specificity; Yeasts | 2019 |
Real-time microscopy and physical perturbation of bacterial pili using maleimide-conjugated molecules.
Topics: Biotin; Caulobacter; Cysteine; Fimbriae, Bacterial; Fluorescent Dyes; Maleimides; Microscopy, Fluorescence; Models, Molecular; Optical Imaging; Staining and Labeling; Vibrio cholerae | 2019 |
Determining the Redox Potential of a Protein Disulphide Bond.
Topics: Biotin; Cysteine; Disulfides; Maleimides; Oxidation-Reduction; Protein Domains; Protein Folding; Proteins; Sulfhydryl Compounds; Tandem Mass Spectrometry | 2019 |
Evaluation of Chemically-Cleavable Linkers for Quantitative Mapping of Small Molecule-Cysteinome Reactivity.
Topics: Azo Compounds; Biotin; Cell Line, Tumor; Cysteine; Humans; Protein Binding; Proteome; Proteomics; Quinazolinones; Silanes | 2019 |
Site-Specific Quantification of Persulfidome by Combining an Isotope-Coded Affinity Tag with Strong Cation-Exchange-Based Fractionation.
Topics: A549 Cells; Biotin; Carbon Isotopes; Chromatography, Ion Exchange; Cysteine; Epithelial-Mesenchymal Transition; HeLa Cells; Humans; Isotope Labeling; Protein Processing, Post-Translational; Proteome; Reference Standards; Serum Albumin, Bovine; Streptavidin; Sulfides; Tandem Mass Spectrometry | 2019 |
Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome.
Topics: Azides; Biotin; Cysteine; Isotope Labeling; Molecular Structure; Staphylococcus aureus | 2020 |
Discovery of a Redox Thiol Switch: Implications for Cellular Energy Metabolism.
Topics: Activating Transcription Factor 4; Animals; Biological Assay; Biotin; Cell Line; Cysteine; Disulfides; Energy Metabolism; Glycolysis; Hepatocytes; Humans; Hydrogen Sulfide; Insulin-Secreting Cells; Mass Spectrometry; Metabolic Flux Analysis; Mitochondria; Oxidation-Reduction; Proteome; Proteomics; Rats; Sulfhydryl Compounds; Sulfides | 2020 |
Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation.
Topics: Azides; Biotin; Blotting, Western; Cell Surface Display Techniques; Chemistry Techniques, Synthetic; Click Chemistry; Cysteine; Fungal Proteins; Gene Expression; Inteins; Protein Engineering; Protein Splicing; Recombinant Proteins; Saccharomyces cerevisiae; Single-Chain Antibodies | 2020 |
Topics: Binding Sites; Biotin; Cysteine; Glutathione; HeLa Cells; HSP70 Heat-Shock Proteins; Humans; Magnetic Resonance Spectroscopy; Mutant Proteins; Mutation; Peptides; Protein Stability; Protein Structure, Secondary; Protein Unfolding; Structure-Activity Relationship; Substrate Specificity | 2020 |
Labeling Antibodies.
Topics: Alzheimer Disease; Amyloid beta-Peptides; Antibodies; Biotin; Carbohydrates; Cross-Linking Reagents; Cysteine; Gold Colloid; Humans; Immunoconjugates; Isotope Labeling; Luminescent Proteins; Staining and Labeling; Sulfhydryl Compounds | 2020 |
SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*.
Topics: Biotin; Cycloaddition Reaction; Cysteine; HEK293 Cells; Humans; Iodoacetamide; Ion Mobility Spectrometry; Peptides; Proteomics; Solid-Phase Synthesis Techniques | 2021 |
A novel posttranslational modification of histone, H3 S-sulfhydration, is down-regulated in asthenozoospermic sperm.
Topics: Amino Acid Sequence; Animals; Asthenozoospermia; Biotin; Cysteine; Gene Expression Regulation; Histones; Humans; Hydrogen Sulfide; Infertility; Male; Mice; Mice, Inbred C57BL; Mutation; Protein Processing, Post-Translational; Spermatogenesis; Spermatozoa; Sulfhydryl Compounds; Sulfides | 2021 |
Crystal structure of the Cys-NO modified YopH tyrosine phosphatase.
Topics: Bacterial Outer Membrane Proteins; Biotin; Catalysis; Crystallography, X-Ray; Cysteine; Humans; Mass Spectrometry; Molecular Structure; Nitric Oxide; Oxidation-Reduction; Protein Tyrosine Phosphatases; Signal Transduction; Yersinia enterocolitica | 2022 |
Biotin alleviates hepatic and intestinal inflammation and apoptosis induced by high dietary carbohydrate in juvenile turbot (Scophthalmus maximus L.).
Topics: Animal Feed; Animals; Apoptosis; bcl-2-Associated X Protein; Biotin; Caspase 3; Cysteine; Diet; Dietary Carbohydrates; Dietary Supplements; Flatfishes; Inflammation; Interleukin-10; Interleukin-1beta; Interleukin-6; Interleukin-8; Liver; NF-kappa B; RNA, Messenger; Transforming Growth Factor beta; Transforming Growth Factors; Tumor Necrosis Factor-alpha | 2022 |
Activity-Based Proteomic Identification of the
Topics: Antioxidants; Biotin; Breast Neoplasms; Cysteine; Disulfides; Female; Garlic; Humans; Proteomics; Sulfoxides | 2022 |
Detection of Cysteine Sulfenic Acid on
Topics: Biotin; Cysteine; Escherichia coli; Escherichia coli Proteins; Oxidation-Reduction; Sulfenic Acids | 2023 |
An efficient site-selective, dual bioconjugation approach exploiting N-terminal cysteines as minimalistic handles to engineer tailored anti-HER2 affibody conjugates.
Topics: Biotin; Cell Line; Cysteine; Esters; Immunoconjugates | 2023 |