cysteine and biotin

cysteine has been researched along with biotin in 233 studies

Research

Studies (233)

TimeframeStudies, this research(%)All Research%
pre-199023 (9.87)18.7374
1990's32 (13.73)18.2507
2000's95 (40.77)29.6817
2010's70 (30.04)24.3611
2020's13 (5.58)2.80

Authors

AuthorsStudies
Novogrodsky, A; Wilchek, M; Wynne, D1
Slyter, LL; Weaver, JM1
Baggiolini, EG; Confalone, PN; Lollar, D; Pizzolato, G; Uskoković, MR2
Drexler, H; Kylberg, KJ1
Bhattacharyya, R; Samanta, TK1
Deziel, MR; Mau, MM1
Bayer, EA; Safars, M; Wilchek, M1
Adamik, R; Bobak, DA; Moss, J; Noda, M; Tsai, SC; Vaughan, M1
Kison, R; Meyer, HE; Schoner, W1
Rudman, D; Williams, PJ1
Drake, HL; Savage, MD1
Ycas, M1
Carlsson, J2
Bal, J; Balbin, E; Pieniazek, NJ1
Alberts, AW; Nervi, AM; Vagelos, PR1
Ilahi, M; Kelemen, MV; Rose, AH1
Duprè, S; Solinas, SP; Spoto, G1
Frappier, F; Marquet, A1
Harrell, AC; Jacobson, ES1
Chen, G; Chilkoti, A; Harris, JM; Hoffman, AS; Long, C; Shimoboji, T; Stayton, PS1
Capaldi, RA; Tang, C; Wilkens, S1
Finkelstein, A; Jakes, KS; Qiu, XQ; Slatin, SL1
Beckwith, J; Guilhot, C; Jander, G; Martin, NL1
Hendrickson, TL; Imperiali, B; Pathak, R1
Casey, PJ; Furfine, ES; Landavazo, A; Leban, JJ; Moomaw, JF1
Cantor, CR; Sano, T; Smith, CL1
Imai, Y; Komatsubara, S; Masuda, M; Sakurai, N; Tosa, T1
Bui, BT; Florentin, D; Izumi, Y; Marquet, A; Ohshiro, T; Yamamoto, M1
Breitling, F; Dübel, S; Kipriyanov, SM; Kontermann, RE; Little, M1
Bui, BT; Florentin, D; Izumi, Y; Marquet, A; Ohshiro, T1
Blume, AJ; Brown, AM; Dushin, RG; George, SM; Jacobsen, JS; Sonnenberg-Reines, J1
Schäfer, H; Schmidt, WE1
Bui, BT; Florentin, D; Izumi, Y; Marquet, A; Méjean, A; Ploux, O1
Frillingos, S; Kaback, HR1
Gravitt, KR; O'Brian, CA; Ward, NE1
Hubbell, WL; Kaback, HR; Voss, J; Wu, J1
Baumgartner, R; Buck, GA; Hymes, J; Norrgard, KJ; Pomponio, RJ; Reynolds, TR; Suormala, T; Wolf, B1
Hagting, A; Konings, WN; Poolman, B; vd Velde, J1
Cynshi, O; Ikeda, S; Kushima, Y; Sugawa, M; Takashima, Y1
Achremowicz, B; Udeh, KO1
Bös, C; Braun, V1
Chen, JG; Liu-Chen, S; Rudnick, G1
Asahara, H; Nishikawa, K; Ohtsuka, H; Yokogawa, T1
Blachly-Dyson, E; Colombini, M; Forte, M; Midson, C; Song, J2
Amara, SG; Leighton, BH; Seal, RP2
Boileau, AJ; Czajkowski, C; Davis, AF; Evers, AR1
Anderluh, G; Barlic, A; Gubensek, F; Macek, P; Menestrina, G; Podlesek, Z; Pungercar, J; Serra, MD; Zecchini, ML1
Begley, TP; Kinsland, C; McLafferty, F; Taylor, S; Xi, J1
Asai, Y; Homma, M; Kawagishi, I; Shoji, T1
Blaustein, RO; Collier, RJ; Finkelstein, A; Gordon, M; Oh, KJ; Senzel, L1
Levert, KL; Lloyd, RB; Waldrop, GL1
Bui, BT; Chottard, G; Escalettes, F; Florentin, D; Marquet, A1
Bures, EJ; Davis, MT; Kroemer, G; McGinley, MD; Patterson, SD; Robinson, JH; Spahr, CS; Susin, SA1
Kim, JR; Kwon, KS; Lee, SR; Rhee, SG; Yoon, HW1
Barber, MJ; Pollock, VV1
Fergusson, MM; Finkel, T; Levine, RL; Sullivan, DM; Wehr, NB1
Czajkowski, C; Wagner, DA1
Franke, I; Gast, FU; Meiss, G; Pingoud, A1
Alving, K; Anderson, DJ; Anderson, GA; Belov, ME; Chrisler, WB; Conrads, TP; Lipton, MS; Pasa-Tolić, L; Smith, RD; Thrall, BD; Udseth, HR; Veenstra, TD1
Bestagno, M; Burrone, OR; Mandiola, PA; Mukherjee, S; Sepúlveda, J; Vangelista, L1
Conti, LR; Radeke, CM; Shyng, SL; Vandenberg, CA1
Marquet, A1
Casey, JR; Taylor, AM; Zhu, Q1
Chiang, HJ; Farh, L; Hwang, SY; Shiuan, D; Steinrauf, L1
Jaffrey, SR; Snyder, SH1
Gross, SS; Hao, G; Lane, P1
Ennion, SJ; Evans, RJ1
Fontecave, M; Hewitson, KS; Münck, E; Ollagnier-de Choudens, S; Roach, P; Sanakis, Y1
Baldwin, JE; Fontecave, M; Hewitson, KS; Münck, E; Ollagnier-de Choudens, S; Roach, PL; Sanakis, Y; Shaw, NM1
Eaton, P; Fuller, W; Shattock, MJ1
Grunewald, M; Kanner, BI; Menaker, D1
Ferrer, JV; Javitch, JA; Kuhn, DM; Park, SU1
Donoghue, N; Hogg, PJ; Jiang, XM; Li, P; Matthias, LJ; Poumbourios, P; Vandegraaff, N; Yam, PT1
Finn, MG; Johnson, JE; Lin, T; Wang, Q1
Hatsuda, M; Mori, Y; Seki, M; Yamada, S1
Finkel, E1
Kobilka, BK; Neumann, L; Whelan, RJ; Wohland, T; Zare, RN1
Hytönen, VP; Kulomaa, MS; Laitinen, OH; Nordlund, HR; Nyholm, T; Slotte, JP; Uotila, ST1
Arora, PS; Eisenführ, A; Famulok, M; Nowick, JS; Sengle, G; Takaoka, LR1
Distefano, MD; Kale, TA1
Goldknopf, IL; Kone, BC; Kuncewicz, T; Sheta, EA1
Handel, S; Jose, J1
Beck-Sickinger, AG; Grouzmann, E; Machova, Z; von Eggelkraut-Gottanka, R1
Crabtree, M; Gross, SS; Hao, G1
Flintoff, WF; Sadlish, H; Williams, FM1
HUNGATE, RE1
NIMURA, T; SAHASHI, Y; SUZUKI, T3
Birk, DE; Brittingham, RJ; Colombo, M; Fertala, A; Klement, JF; Majsterek, I; Uitto, J1
Garfield, S; Gottesman, MM; Liang, XJ; Shen, DW1
Kim, JE; Tannenbaum, SR1
Duell, K; Konopka, JB; Lin, JC1
Chen, GY; Lesaicherre, ML; Lue, RY; Yao, SQ; Zhu, Q1
Chen, GY; Lesaicherre, ML; Uttamchandani, M; Yao, SQ1
Chillarón, J; Gasol, E; Jiménez-Vidal, M; Palacín, M; Zorzano, A1
Conrads, TP; Hood, BL; Kim, BJ; Song, BJ; Suh, SK; Veenstra, TD1
Hatsuda, M; Mori, Y; Seki, M; Shimizu, T; Yamada, S; Yoshida, S1
Bolzati, C; Cavazza-Ceccato, M; Marotta, E; Porchia, M; Refosco, F; Tisato, F; Tomasin, P; Traldi, P; Tubaro, M1
Czajkowski, C; McDevitt, RA; Newell, JG1
Arbabian, M; Artemyev, NO; Grant, JE; Guo, LW; Hajipour, AR; Muradov, H; Ruoho, AE1
Greenberg, MM; Sato, K1
Broniowska, KA; Hogg, N; Keszler, A; Zhang, Y1
Cataldi, TR; Nardiello, D1
González de Orduña, C; Higueras, MA; Lamas, S; López-Ferrer, D; Martínez-Ruiz, A; Rodríguez-Crespo, I; Tarín, C; Vázquez, J; Villanueva, L1
Chen, SS; Yan, W1
Freeman, ML; Hong, F; Liebler, DC; Sekhar, KR1
Berzat, AC; Buss, JE; Chenette, EJ; Cox, AD; Der, CJ; Minden, A; Shutes, A; Weinbaum, CA1
Dahout, C; Gallagher, M; Garin, J; Gonzalez de Peredo, A; Ramus, C1
Huri, DA; Kim, SF; Snyder, SH1
Dennehy, MK; Liebler, DC; Richards, KA; Shyr, Y; Wernke, GR1
Seki, M1
Adams, DG; Codreanu, SG; Dawson, ES; Liebler, DC; Wadzinski, BE1
Calder, G; Evans, DJ; Lomonossoff, GP; Steinmetz, NF1
Marletta, MA; Mitchell, DA; Morton, SU1
Dangprapai, Y; Pelis, RM; Wright, SH; Wunz, TM1
Forrester, MT; Foster, MW; Stamler, JS1
Cheng, P; Gregson, MW; Hackel, BJ; Kapila, A; Lipovsek, D; Lippow, SM; Wittrup, KD1
Chattopadhaya, S; Tan, LP; Yao, SQ1
Liebler, DC; Orton, CR1
Liebler, DC; Liu, Q; Shin, NY; Stamer, SL1
Anderson, DE; Chen, X; Kirk, KL; Shim, JY; Yang, F; Zhang, X1
Hanck, DA; Sheets, MF1
Cummins, TR1
Agostini, S; Bandoli, G; Bolzati, C; Caporale, A; Carta, D; Cavazza-Ceccato, M; Refosco, F; Schievano, E; Tisato, F1
Derakhshan, B; Gross, SS; Wille, PC1
Myers, AG; Siegrist, R; Wulff, JE1
Eggler, AL; Liu, D; Liu, G; Luo, Y; Mesecar, AD; van Breemen, RB1
Kamo, N; Kikukawa, T; Kouyama, T; Kurata, Y; Miyauchi, S; Nara, T1
Gladwin, MT; Hogg, N; Kettenhofen, NJ; Shiva, S; Wang, X1
Bhakta, S; Eigenbrot, C; Ervin, KE; Junutula, JR; Lowman, HB; Raab, H; Scheller, RH; Vandlen, R1
Chen, C; Han, P; Huang, B; Zhang, X; Zhou, X1
Imaizumi, K; Kimura, M; Masuda, Y; Matsuoka, R; Muto, A; Sato, M1
Fang, J; Iwasa, KH; Marriott, G; Sakata, T1
Borderie, D; Ekindjian, OG1
Corjon, S; Engler, T; Kochanek, S; Kreppel, F; van Rooijen, N; Wortmann, A1
Bachi, A; Malgaroli, A; Torta, F; Usuelli, V1
Briceño, J; Corrales, FJ; De La Mata, M; Ferrín, G; Gómez, MA; González, R; Hidalgo, AB; López-Cillero, P; López-Sánchez, LM; Muñoz-Castañeda, JR; Muntané, J; Ranchal, I; Rodríguez-Ariza, A1
Benhar, M; Forrester, MT; Foster, MW; Stamler, JS1
de la Mata, M; López-Sánchez, LM; Muntané, J; Rodríguez-Ariza, A1
Campos, SK; Ozbun, MA1
Yan, LJ1
Blonski, C; Chaisemartin, L; Chinestra, P; Favre, G; Faye, JC1
Forrester, MT; Foster, MW; Moseley, MA; Nogueira, L; Stamler, JS; Thompson, JW1
Abu Bakar, FB; Chattopadhaya, S; Yao, SQ1
Perkel, JM1
Bossy, B; Bossy-Wetzel, E; Chen, J; Klinglmayr, E; Knott, AB; Lütz-Meindl, U; Masliah, E; Petrilli, A; Schwarzenbacher, R1
Crawford, PA; Ivanenkov, VV; Kirley, TL; Sévigny, J; Toyama, A1
Chandrasena, RE; Edirisinghe, PD; Schiefer, IT; Sinha, V; Thatcher, GR; Wijewickrama, GT; Xu, H1
Fujishima, SH; Hamachi, I; Nonaka, H; Ojida, A; Uchinomiya, SH1
Chen, D; Deng, J; Gong, W; Hou, J; Wang, X; Xie, Z; Yang, F; Zhao, Z1
Chen, Y; Chung, KK; Fu, AK; Fu, WY; Ip, NY; Tsang, AH; Yu, PC; Zhang, P1
Nishiya, T; Uehara, T1
Burgoyne, JR; Eaton, P; Mayr, M; Rudyk, O1
Kazuta, Y; Matsuura, T; Yanagida, H; Yomo, T1
Branchaud, BP; Ying, LQ1
Hakalahti, L; Heikkinen, JJ; Hormi, OE; Hytönen, VP; Kivimäki, L; Kulomaa, MS; Määttä, JA; Mäkelä, I; Takkinen, K1
Burns, RN; Moniri, NH1
Clarke, DJ; Langridge-Smith, PR; Scotcher, J1
Brownfield, MS; Elfarra, AA; Irving, RM1
Ghosal, A; Said, HM1
Hytönen, VP; Jänis, J; Kulomaa, MS; Määttä, JA; Niederhauser, B; Siivonen, J1
Cole, RN; Murray, CI; O'Meally, RN; Uhrigshardt, H; Van Eyk, JE1
Chang, TC; Chien, WT; Jan, FD; Kuo, YY; Liang, CF; Lin, CC; Wu, HT; Yu, CC1
Inouye, S; Sato, J1
Bolbach, G; Clavier, S; Diebate, O; Guianvarc'h, D; Karoyan, P; Matheron, L; Sachon, E1
Chen, X; Cui, L; Guan, T; Kong, J; Li, C; Li, XM; Shang, H; Zhang, X1
Murray, CI; Van Eyk, JE1
Bhatnagar, A; Desnoyer, R; Jagannathan, R; Karnik, SS; Tirupula, K; Unal, H1
Berchner-Pfannschmidt, U; de Groot, H; Fandrey, J; Kirsch, M; Petrat, F; Wiesweg, M1
Daaka, Y; Dey, A; Qin, Y1
Gamper, N; Gigout, S; Ooi, L; Pettinger, L1
Chung, HS; Murray, CI; Uhrigshardt, H; Van Eyk, JE1
Hirakawa, H; Nagamune, T; Ohashi, H; Oshiba, Y; Tamaki, T; Yamaguchi, S; Yamaguchi, T1
Kaji, T; Kumagai, Y; Shinkai, Y; Toyama, T1
Avery, VM; Fechner, GA; Lock, A; Neve, J; Stevenson, GI; Yong, S1
Bai, L; Cao, Y; Chai, Y; Wang, H; Xiao, L; Yuan, R1
Ilyas, M; Ilyas, S; Mathur, S; van der Hoorn, RA1
Ahmed, N; Asif, AR; Khan, R; Oellerich, M; Zahid, S1
Carroll, KS; Devarie-Baez, NO; Filipovic, MR; Macinkovic, I; Pan, J; Park, CM; Xian, M; Zhang, D1
Cassia, R; Delena, RA; Lamattina, L; Tavares, CP; Terenzi, H; Vernal, J1
Fago, A; Gow, AJ; Helbo, S; Howes, BD; Jamil, A; Smulevich, G1
Ha, HJ; Ha, S; Kim, HJ; Lee, HY; Lee, JJ; Lee, KJ1
Ohno, K; Okuda, K; Uehara, T1
Filipovic, MR; Macinkovic, I; Park, CM; Xian, M1
Paul, BD; Snyder, SH1
Huang, J; Kast, J; Li, R1
Aroca, Á; Gotor, C; Romero, LC; Serna, A1
Gonzalez, P; Green, KN; Rodich, S; Scarborough, JH1
Carpenter, EP; Liesa, M; Qiu, W; Shirihai, OS1
Balkan, W; Bomgarden, RD; Chung, HS; Cole, RN; Crowgey, EL; Hare, JM; Kass, DA; Murray, CI; Rainer, PP; Rogers, JC; Van Eyk, JE; Venkatraman, V1
Fogen, D; Ng, KK; Wong, SL; Wu, SC1
Gottlieb, CD; Linder, ME; Zhang, S1
Seth, D; Stamler, JS1
Liu, WR; Tharp, JM1
Magwamba, CC; Palittapongarnpim, P; Rukseree, K1
Akaike, T; Fujii, S; Kasamatsu, S1
Johnstone, SR; Lohman, AW; Straub, AC1
Kast, J; Li, R1
Demizu, Y; Hattori, T; Kurihara, M; Misawa, T; Naito, M; Okitsu, K; Shoda, T1
Commandeur, U; Hu, H; Le, DHT; Steinmetz, NF1
de Jong, A; Flierman, D; Kooij, R; Ovaa, H; Witting, K1
Campuzano, IDG; Kilgour, DPA; Lippens, JL; Loo, JA; Netirojjanakul, C; Nshanian, M; Van Orden, S1
Bauer, MS; Gaub, HE; Kluger, C; Milles, LF; Pippig, DA; Schendel, LC; Sedlak, SM1
Chen, X; Cheng, B; Du, Y; Fan, X; Han, M; Hong, W; Huang, R; Liu, Y; Lv, P; Peng, L; Qin, K; Qin, W; Wang, C; Zhou, W; Zhu, Y1
Cabrera-García, JD; Izquierdo-Álvarez, A; Martínez-Ruiz, A; Tello, D1
Aimé, S; Hichami, S; Lamotte, O; Wendehenne, D1
Lindermayr, C; Shekariesfahlan, A1
Chen, Z; Cole, PA; Dempsey, DR; Jiang, H; Kalin, JH1
Bai, Y; Fu, Q; Huff-Lonergan, E; Liu, R; Lonergan, S; Xing, L; Zhang, L; Zhang, W; Zhou, G1
Cheng, R; DeGrado, WF; Li, S; Liu, J; Rozovsky, S; Wang, L; Wang, PG; Wu, H1
Huang, X; Kroetsch, A; Nguyen, VP; Ogoke, O; Parashurama, N; Park, S; Zhou, F1
Brun, YV; Dalia, AB; Dalia, TN; Ellison, CK1
Cook, KM1
Baranczak, A; Bogdan, AR; Rabalski, AJ1
Hu, Y; Li, X; Liang, Z; Shan, Y; Weng, Y; Wu, Q; Yuan, H; Zhang, L; Zhang, Y; Zhao, B1
Hacker, SM; Lewald, L; Zanon, PRA1
Abbott, D; Arvan, P; Bederman, I; Cameron, M; Chance, M; Chirieleison, SM; Gao, XH; Gao, Z; Hatzoglou, M; Krokowski, D; Li, L; Parisien, M; Wang, B; Willard, B; Wu, J1
Shusta, EV; Umlauf, BJ1
Chen, C; Chen, X; Gong, W; Hu, W; Liu, Z; Perrett, S; Wang, L; Wu, H; Wu, S; Yang, J; Zhang, H1
Berg, EA; Fishman, JB1
Backus, KM; Boatner, LM; Cao, J; Desai, HS; Jami-Alahmadi, Y; Palafox, MF; Yan, T; Yen, SL1
Diao, H; Jiang, N; Li, R; Pan, H; Qi, Q; Sun, S; Wan, X; Wang, J; Zhang, F; Zhang, L; Zhang, M1
Brandão-Neto, J; D'Muniz Pereira, H; Martins, PGA; Menegatti, ACO; Rocha, RF; Terenzi, H; Thiemann, OH1
Huang, D; Li, X; Liu, D; Liu, J; Liu, Y; Luo, K; Mai, K; Pan, M; Wu, Z; Zhang, W1
Hunter, R; Kaschula, CH; Katz, AA; Kusza, DA; Schäfer, G; Smith, M1
Bai, Q; Ma, G; Wang, H1
Chen, Z; Cheng, X; Jiang, Z; Liu, G; Niu, Y; Tang, B; Tong, L; Yang, Y; Zhang, G1
Aucagne, V; Berger, S; Kersaudy, F; Lefoulon, F; Morisset-Lopez, S; Novak, A; Pifferi, C1

Reviews

13 review(s) available for cysteine and biotin

ArticleYear
Nutrient deficiencies during total parenteral nutrition.
    Nutrition reviews, 1985, Volume: 43, Issue:1

    Topics: Adolescent; Adult; Aged; Animals; Biotin; Carnitine; Child; Choline Deficiency; Chromium; Copper; Cysteine; Deficiency Diseases; Dogs; Fatty Acids, Essential; Female; Folic Acid Deficiency; Guinea Pigs; Humans; Infant; Male; Middle Aged; Molybdenum; Parenteral Nutrition; Parenteral Nutrition, Total; Phosphates; Rats; Selenium; Sheep; Taurine; Tyrosine; Vitamin A Deficiency; Zinc

1985
The enzymology of sulfur activation during thiamin and biotin biosynthesis.
    Current opinion in chemical biology, 1999, Volume: 3, Issue:5

    Topics: Biotin; Cysteine; Models, Chemical; Phosphotransferases (Alcohol Group Acceptor); Sulfur; Sulfurtransferases; Thiamine

1999
Enzymology of carbon-sulfur bond formation.
    Current opinion in chemical biology, 2001, Volume: 5, Issue:5

    Topics: Acetyltransferases; Anaerobiosis; Bacterial Proteins; Biotin; Cysteine; Enzymes; Intramolecular Transferases; Iron-Sulfur Proteins; Models, Chemical; S-Adenosylmethionine; Sulfur; Sulfurtransferases

2001
S-nitrosylation is emerging as a specific and fundamental posttranslational protein modification: head-to-head comparison with O-phosphorylation.
    Science's STKE : signal transduction knowledge environment, 2001, Jun-12, Volume: 2001, Issue:86

    Topics: Animals; Argininosuccinate Synthase; Biotin; Biotinylation; Cysteine; Humans; Nitric Oxide; Phosphorylation; Protein Processing, Post-Translational; Signal Transduction; Spectrometry, Mass, Electrospray Ionization

2001
Mass spectrometry-based quantitative proteomic profiling.
    Briefings in functional genomics & proteomics, 2005, Volume: 4, Issue:1

    Topics: Biotin; Cysteine; Electrophoresis, Gel, Two-Dimensional; Ions; Mass Spectrometry; Peptides; Protein Structure, Tertiary; Proteomics; Software; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

2005
Biological significance and development of practical synthesis of biotin.
    Medicinal research reviews, 2006, Volume: 26, Issue:4

    Topics: Animals; Avidin; Biotin; Catalysis; Cysteine; Hypoglycemic Agents; Insulin; Insulin Secretion; Iron; Lactones; Models, Biological; Models, Chemical; Pimelic Acids; Rhizobium

2006
Assessment of the nitrosylation process.
    Current opinion in clinical nutrition and metabolic care, 2008, Volume: 11, Issue:5

    Topics: Biotin; Blotting, Western; Colorimetry; Cysteine; Luminescent Measurements; Mass Spectrometry; Nitric Oxide; Nitrosation; Proteins; Spectrometry, Fluorescence; Sulfhydryl Reagents

2008
Proteomic analysis of protein S-nitrosylation.
    Proteomics, 2008, Volume: 8, Issue:21

    Topics: Animals; Biotin; Cysteine; Electrophoresis, Gel, Two-Dimensional; Humans; Mass Spectrometry; Nitric Oxide; Protein Processing, Post-Translational; Proteins; Proteomics; Signal Transduction

2008
Detection of protein S-nitrosylation with the biotin-switch technique.
    Free radical biology & medicine, 2009, Jan-15, Volume: 46, Issue:2

    Topics: Animals; Biotin; Cell Extracts; Chemistry Techniques, Analytical; Cysteine; Humans; Nitric Oxide; Oxidation-Reduction; Protein Processing, Post-Translational; S-Nitrosothiols; Signal Transduction; Sulfhydryl Compounds

2009
Unraveling the S-nitrosoproteome: tools and strategies.
    Proteomics, 2009, Volume: 9, Issue:4

    Topics: Animals; Biotin; Cell Communication; Cysteine; Humans; Mice; Nitric Oxide; Nitrosation; Oxidation-Reduction; Protein Processing, Post-Translational; Proteins; Proteomics; Rats; S-Nitrosothiols; Sulfhydryl Compounds

2009
Screening systems for the identification of S-nitrosylated proteins.
    Nitric oxide : biology and chemistry, 2011, Aug-01, Volume: 25, Issue:2

    Topics: Binding Sites; Biotin; Computational Biology; Cysteine; Disulfides; Mesylates; Nitric Oxide; Oxidation-Reduction; Protein Array Analysis; Protein Processing, Post-Translational; Proteins; Proteomics; S-Nitrosothiols; Substrate Specificity

2011
Protein s-nitrosylation measurement.
    Methods in enzymology, 2013, Volume: 522

    Topics: Antibodies; Biotin; Cysteine; Humans; Luminescent Measurements; Mass Spectrometry; Nitric Oxide; Protein Processing, Post-Translational; Receptors, G-Protein-Coupled; S-Nitrosothiols; Signal Transduction; Staining and Labeling

2013
The biological functions of protein S-sulfhydration in eukaryotes and the ever-increasing understanding of its effects on bacteria.
    Microbiological research, 2023, Volume: 271

    Topics: Animals; Bacteria; Biotin; Cysteine; Eukaryota; Hydrogen Sulfide; Mammals; Protein Processing, Post-Translational; Protein S

2023

Other Studies

220 other study(ies) available for cysteine and biotin

ArticleYear
A chemical approach for the localization of membrane sites involved in lymphocyte activation.
    Biochemical and biophysical research communications, 1976, Feb-09, Volume: 68, Issue:3

    Topics: Animals; Avidin; Binding Sites; Biotin; Cell Aggregation; Cell Membrane; Cysteine; Dinitrophenols; DNA; DNA Replication; Hydrazines; Lymphocyte Activation; Mice; Mice, Inbred Strains; Mitogens; Periodic Acid; T-Lymphocytes

1976
Tetrahydrofolate and other growth requirements of certain strains of Ruminococcus flavefaciens.
    Applied and environmental microbiology, 1977, Volume: 33, Issue:2

    Topics: Acetates; Amino Acids; Ammonia; Animals; Biotin; Butyrates; Carbon Dioxide; Cattle; Culture Media; Cysteine; Peptococcaceae; Rumen; Tetrahydrofolates; Thiamine; Valerates

1977
Stereospecific total synthesis of d-biotin from L(+)-cysteine.
    Journal of the American Chemical Society, 1977, Oct-12, Volume: 99, Issue:21

    Topics: Biotin; Cysteine; Methods; Stereoisomerism

1977
Effect of UV irradiation on transduction by coliphage T1.
    Journal of virology, 1975, Volume: 16, Issue:2

    Topics: Arginine; Biotin; Coliphages; Cysteine; DNA Viruses; Drug Resistance, Microbial; Escherichia coli; Galactose; Genetic Linkage; Lactose; Lysogeny; Mutation; Proline; Radiation Effects; Streptomycin; Transduction, Genetic; Tryptophan; Ultraviolet Rays

1975
Letter: A'stereospecific total synthesis of d-biotin from L-(+)-cysteine.
    Journal of the American Chemical Society, 1975, Oct-01, Volume: 97, Issue:20

    Topics: Biotin; Cysteine; Stereoisomerism

1975
L-lysine production by S-2-aminoethyl-L-cysteine-resistant mutants of Arthrobacter globiformis.
    Folia microbiologica, 1991, Volume: 36, Issue:1

    Topics: Arthrobacter; Biotin; Culture Media; Cysteine; Drug Resistance, Microbial; Glucose; Lysine; Methylnitronitrosoguanidine; Mutagenesis; Mutation; Nitrogen; Species Specificity

1991
Biotin-conjugated reagents as site-specific probes of membrane protein structure: application to the study of the human erythrocyte hexose transporter.
    Analytical biochemistry, 1990, Nov-01, Volume: 190, Issue:2

    Topics: Biotin; Carrier Proteins; Cysteine; Erythrocyte Membrane; Galactose Oxidase; Hexoses; Humans; Hydrazines; Hydrolysis; Maleimides; Membrane Proteins; Serine Endopeptidases; Trypsin

1990
Selective labeling of sulfhydryls and disulfides on blot transfers using avidin-biotin technology: studies on purified proteins and erythrocyte membranes.
    Analytical biochemistry, 1987, Volume: 161, Issue:2

    Topics: Avidin; Biotin; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Erythrocyte Membrane; Humans; Lysine; Maleimides; Membrane Proteins; Mercaptoethanol; Proteins; Staining and Labeling; Sulfhydryl Compounds

1987
Activation of immobilized, biotinylated choleragen AI protein by a 19-kilodalton guanine nucleotide-binding protein.
    Biochemistry, 1989, Sep-19, Volume: 28, Issue:19

    Topics: Biotin; Cholera Toxin; Cysteine; Enzyme Activation; GTP-Binding Proteins; Molecular Weight; NAD; Poly(ADP-ribose) Polymerases

1989
Characterization of a cysteine-containing peptide after affinity labelling of Ca2+-ATPase of sarcoplasmic reticulum with the disulfide of 3'(2')-O-biotinyl-thioinosine triphosphate.
    European journal of biochemistry, 1989, May-01, Volume: 181, Issue:2

    Topics: Affinity Labels; Amino Acid Sequence; Animals; Biotin; Calcium; Calcium-Transporting ATPases; Cysteine; Kinetics; Molecular Sequence Data; Peptide Fragments; Rabbits; Sarcoplasmic Reticulum; Thionucleotides; Trypsin

1989
Adaptation of the acetogen Clostridium thermoautotrophicum to minimal medium.
    Journal of bacteriology, 1986, Volume: 165, Issue:1

    Topics: Acetates; Acetic Acid; Adaptation, Physiological; Biotin; Clostridium; Culture Media; Cysteine; Glucose; Methanol; Niacin

1986
On earlier states of the biochemical system.
    Journal of theoretical biology, 1974, Volume: 44, Issue:1

    Topics: Amino Acid Sequence; Bacillus cereus; Bacterial Proteins; Biological Evolution; Biotin; Coenzyme A; Cysteine; Enzymes; Escherichia coli; Genetic Code; Glucosephosphate Dehydrogenase; Ketoglutarate Dehydrogenase Complex; Methionine; Molecular Biology; Multienzyme Complexes; Mutation; Penicillins; Peptide Chain Termination, Translational; Pyruvate Dehydrogenase Complex

1974
Nutritional requirements of Streptococcus sanguis.
    Archives of oral biology, 1972, Volume: 17, Issue:9

    Topics: Biotin; Culture Media; Cysteine; Glucose; Mouth; Nicotinic Acids; Pantothenic Acid; Pyridoxine; Riboflavin; Spectrophotometry; Streptococcus; Thiamine; Tooth

1972
Method for isolating auxotrophic mutants in Aspergillus nidulans using N-glycosyl-polifungin.
    Journal of general microbiology, 1974, Volume: 84, Issue:1

    Topics: Aminobenzoates; Antifungal Agents; Aspergillus nidulans; Biotin; Cell Survival; Cysteine; Mutation; Nucleic Acids; Polyenes; Spores, Fungal; Ultraviolet Rays

1974
Acetyl CoA carboxylase. 3. Purification and properties of a biotin carboxyl carrier protein.
    Archives of biochemistry and biophysics, 1971, Volume: 143, Issue:2

    Topics: Amino Acids; Bacterial Proteins; Benzoates; Biotin; Catalysis; Chemical Phenomena; Chemistry; Chemistry, Physical; Chromatography, DEAE-Cellulose; Chromatography, Gel; Coenzyme A; Crystallization; Cysteine; Detergents; Diffusion; Electrophoresis, Disc; Escherichia coli; Ethanol; Ligases; Methods; Protein Binding; Protein Denaturation; Quaternary Ammonium Compounds; Sulfates; Sulfhydryl Compounds; Sulfides; Sulfuric Acids; Ultracentrifugation; Urea

1971
Studies on the biosynthesis of biotin. Production of biotin and biotin-like compounds by a pseudomonad.
    The Biochemical journal, 1965, Volume: 96, Issue:2

    Topics: Amino Acids; Biotin; Chromatography, Paper; Culture Media; Cysteine; Cystine; In Vitro Techniques; Lysine; Malonates; Pimelic Acids; Pseudomonas aeruginosa

1965
Nutritional requirements of Streptococcus salivarius.
    Journal of general microbiology, 1971, Volume: 67, Issue:1

    Topics: Ammonia; Biotin; Culture Media; Cysteine; Cystine; Glucose; Glutamates; Homocysteine; Homocystine; Humans; Mouth; Nicotinic Acids; Pantothenic Acid; Riboflavin; Streptococcus; Thiamine; Urea

1971
Cysteine as precursor and sulfur donor in the biosynthesis of natural sulfur-containing compounds.
    Progress in clinical and biological research, 1983, Volume: 125

    Topics: Animals; Biotin; Cysteine; Ergothioneine; Glutathione; Thiamine; Thioctic Acid; Tissue Distribution

1983
On the biosynthesis of biotin in Achromobacter IVSW. A reinvestigation.
    Biochemical and biophysical research communications, 1981, Dec-31, Volume: 103, Issue:4

    Topics: Alcaligenes; Biotin; Carbon Radioisotopes; Cysteine; Radioisotope Dilution Technique; Sulfur Radioisotopes; Tritium

1981
A prototrophic yeast-strain of Histoplasma capsulatum.
    Mycopathologia, 1982, Feb-19, Volume: 77, Issue:2

    Topics: Animals; Biotin; Culture Media; Cysteine; Glucose; Histoplasma; Histoplasmosis; Mice; Sodium; Sulfates

1982
Control of protein-ligand recognition using a stimuli-responsive polymer.
    Nature, 1995, Nov-30, Volume: 378, Issue:6556

    Topics: Acrylic Resins; Bacterial Proteins; Biotin; Cysteine; Cytochromes b5; Ligands; Models, Molecular; Mutagenesis, Site-Directed; Polymers; Protein Binding; Protein Conformation; Recombinant Proteins; Streptavidin; Sulfones; Temperature

1995
Structure of the gamma subunit of Escherichia coli F1 ATPase probed in trypsin digestion and biotin-avidin binding studies.
    The Journal of biological chemistry, 1994, Feb-11, Volume: 269, Issue:6

    Topics: Amino Acid Sequence; Antibodies, Monoclonal; Avidin; Biotin; Cysteine; Epitopes; Molecular Sequence Data; Peptide Mapping; Protein Structure, Secondary; Proton-Translocating ATPases; Structure-Activity Relationship; Trypsin

1994
Identification of a translocated protein segment in a voltage-dependent channel.
    Nature, 1994, Sep-08, Volume: 371, Issue:6493

    Topics: Amino Acid Sequence; Bacterial Proteins; Biological Transport; Biotin; Colicins; Cysteine; Electrophysiology; Ion Channel Gating; Ion Channels; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Protein Conformation; Streptavidin

1994
Evidence that the pathway of disulfide bond formation in Escherichia coli involves interactions between the cysteines of DsbB and DsbA.
    Proceedings of the National Academy of Sciences of the United States of America, 1995, Oct-10, Volume: 92, Issue:21

    Topics: Bacterial Proteins; Biotin; Blotting, Western; Cell Fractionation; Cysteine; Disulfides; Escherichia coli; Genes, Bacterial; Genes, Dominant; Isomerases; Membrane Proteins; Mutagenesis; Oxidation-Reduction; Protein Binding; Protein Disulfide-Isomerases; Recombinant Proteins

1995
Sulfhydryl modification of the yeast Wbp1p inhibits oligosaccharyl transferase activity.
    Biochemistry, 1995, Apr-04, Volume: 34, Issue:13

    Topics: Amino Acid Sequence; Biotin; Carbohydrate Sequence; Catalysis; Cysteine; Dithiothreitol; Enzyme Stability; Hexosyltransferases; Kinetics; Macromolecular Substances; Membrane Proteins; Mesylates; Methyl Methanesulfonate; Microsomes; Molecular Sequence Data; Polyisoprenyl Phosphate Oligosaccharides; Saccharomyces cerevisiae; Structure-Activity Relationship; Sulfhydryl Compounds; Transferases

1995
Protein farnesyltransferase: kinetics of farnesyl pyrophosphate binding and product release.
    Biochemistry, 1995, May-23, Volume: 34, Issue:20

    Topics: Alkyl and Aryl Transferases; Amino Acid Sequence; Binding Sites; Biotin; Catalysis; Cysteine; Kinetics; Molecular Sequence Data; Peptides; Polyisoprenyl Phosphates; Protein Prenylation; Sesquiterpenes; Spectrometry, Fluorescence; Transferases

1995
A streptavidin mutant containing a cysteine stretch that facilitates production of a variety of specific streptavidin conjugates.
    Bio/technology (Nature Publishing Company), 1993, Volume: 11, Issue:2

    Topics: Amino Acid Sequence; Animals; Bacterial Proteins; Base Sequence; Biotin; Cell Membrane; Cloning, Molecular; Cysteine; DNA, Recombinant; Escherichia coli; Fluoresceins; Genetic Vectors; Methylation; Mice; Molecular Sequence Data; Streptavidin; Sulfhydryl Compounds; Tumor Cells, Cultured

1993
Improvement of a d-biotin-hyperproducing recombinant strain of Serratia marcescens.
    Journal of biotechnology, 1994, Jul-29, Volume: 36, Issue:1

    Topics: Biotin; Cysteine; Ethionine; Plasmids; Recombinant Proteins; Serratia marcescens

1994
Stimulatory factors for enzymatic biotin synthesis from dethiobiotin in cell-free extracts of Escherichia coli.
    Bioscience, biotechnology, and biochemistry, 1995, Volume: 59, Issue:5

    Topics: Bacillus; Biotin; Cell Extracts; Cysteine; Escherichia coli; Flavin-Adenine Dinucleotide; Immunosuppressive Agents; NAD; NADP; S-Adenosylmethionine

1995
Recombinant single-chain Fv fragments carrying C-terminal cysteine residues: production of bivalent and biotinylated miniantibodies.
    Molecular immunology, 1994, Volume: 31, Issue:14

    Topics: Amino Acid Sequence; Animals; Antibodies, Bispecific; Antibody Affinity; Base Sequence; Biotin; Blotting, Western; Chromatography, Affinity; Cysteine; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Immunoglobulin Fragments; Immunoglobulin Variable Region; Mice; Molecular Sequence Data; Recombinant Proteins

1994
On the mechanism of biotin synthase of Bacillus sphaericus.
    Comptes rendus de l'Academie des sciences. Serie III, Sciences de la vie, 1994, Volume: 317, Issue:6

    Topics: Bacillus; Biotin; Cysteine; S-Adenosylmethionine; Sulfurtransferases

1994
Biotinylated and cysteine-modified peptides as useful reagents for studying the inhibition of cathepsin G.
    Analytical biochemistry, 1994, Feb-15, Volume: 217, Issue:1

    Topics: alpha 1-Antichymotrypsin; Amino Acid Sequence; Biotin; Cathepsin G; Cathepsins; Cysteine; Ethylmaleimide; Hydrogen-Ion Concentration; Kinetics; Molecular Sequence Data; Peptides; Reproducibility of Results; Sensitivity and Specificity; Serine Endopeptidases; Tritium

1994
Characterization and purification of the solubilized pituitary adenylate-cyclase-activating polypeptide-1 receptor from porcine brain using a biotinylated ligand.
    European journal of biochemistry, 1993, Nov-01, Volume: 217, Issue:3

    Topics: Animals; Biotin; Brain; Chromatography, Affinity; Cysteine; Electrophoresis, Polyacrylamide Gel; Glycosylation; Ligands; Neuropeptides; Radioligand Assay; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; Receptors, Pituitary Hormone; Swine

1993
Highly purified biotin synthase can transform dethiobiotin into biotin in the absence of any other protein, in the presence of photoreduced deazaflavin.
    Biochemical and biophysical research communications, 1995, Dec-26, Volume: 217, Issue:3

    Topics: Amino Acid Sequence; Bacillus; Biotin; Cell-Free System; Cysteine; Flavins; Molecular Sequence Data; Spectrum Analysis; Sulfur; Sulfurtransferases

1995
Probing the conformation of the lactose permease of Escherichia coli by in situ site-directed sulfhydryl modification.
    Biochemistry, 1996, Apr-02, Volume: 35, Issue:13

    Topics: Amino Acid Sequence; Biotin; Carbohydrate Sequence; Cell Membrane; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Kinetics; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Plasmids; Point Mutation; Protein Conformation; Recombinant Proteins; Sulfhydryl Compounds; Symporters; Thiogalactosides; Valine

1996
Covalent modification of protein kinase C isozymes by the inactivating peptide substrate analog N-biotinyl-Arg-Arg-Arg-Cys-Leu-Arg-Arg-Leu. Evidence that the biotinylated peptide is an active-site affinity label.
    The Journal of biological chemistry, 1996, Sep-27, Volume: 271, Issue:39

    Topics: Animals; Binding Sites; Biotin; Brain; Calcium; Cysteine; Dithiothreitol; Enzyme Inhibitors; Isoenzymes; Oligopeptides; Phosphatidylserines; Protein Binding; Protein Kinase C; Rats

1996
Site-directed spin labeling and chemical crosslinking demonstrate that helix V is close to helices VII and VIII in the lactose permease of Escherichia coli.
    Proceedings of the National Academy of Sciences of the United States of America, 1996, Sep-17, Volume: 93, Issue:19

    Topics: Amino Acid Sequence; Biotin; Cell Membrane; Cysteine; Cytoplasm; Electron Spin Resonance Spectroscopy; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Membrane Transport Proteins; Models, Structural; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Recombinant Proteins; Spin Labels; Symporters

1996
Arg538 to Cys mutation in a CpG dinucleotide of the human biotinidase gene is the second most common cause of profound biotinidase deficiency in symptomatic children.
    Human genetics, 1997, Volume: 99, Issue:4

    Topics: Acyltransferases; Alleles; Amidohydrolases; Antibodies; Arginine; Automation; Biotin; Biotinidase; Cells, Cultured; Child; Cysteine; Dinucleoside Phosphates; DNA; Exons; Female; Fibroblasts; Humans; Male; Pedigree; Point Mutation; Sequence Analysis, DNA

1997
Membrane topology of the di- and tripeptide transport protein of Lactococcus lactis.
    Biochemistry, 1997, Jun-03, Volume: 36, Issue:22

    Topics: Alkaline Phosphatase; Amino Acid Sequence; Animals; Bacterial Proteins; Base Sequence; Biotin; Carrier Proteins; Cell Membrane; Cysteine; Dipeptides; Escherichia coli; Humans; Lactococcus lactis; Membrane Transport Proteins; Models, Chemical; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptides; Plasmids; Polymerase Chain Reaction; Protein Structure, Secondary; Recombinant Fusion Proteins; Sequence Alignment

1997
Oxidized low density lipoprotein caused CNS neuron cell death.
    Brain research, 1997, Jun-27, Volume: 761, Issue:1

    Topics: Animals; Antidotes; Antioxidants; Biotin; Cell Death; Cells, Cultured; Cerebral Cortex; Cysteine; Deoxyuracil Nucleotides; Dithiothreitol; DNA Fragmentation; Female; Fetus; Glutathione; Hippocampus; Humans; Lipoproteins, LDL; Neuroblastoma; Oxidation-Reduction; Oxidative Stress; Rabbits; Rats; Rats, Wistar; Staining and Labeling; Sulfhydryl Reagents; Thiobarbituric Acid Reactive Substances; Tumor Cells, Cultured; Vitamin E

1997
High-glutathione containing yeast Saccharomyces cerevisiae: optimization of production.
    Acta microbiologica Polonica, 1997, Volume: 46, Issue:1

    Topics: Biomass; Biotin; Culture Media; Cysteine; Glucose; Glutathione; Peptones; Phosphates; Potassium Compounds; Saccharomyces cerevisiae

1997
Specific in vivo thiol-labeling of the FhuA outer membrane ferrichrome transport protein of Escherichia coli K-12: evidence for a disulfide bridge in the predicted gating loop.
    FEMS microbiology letters, 1997, Aug-15, Volume: 153, Issue:2

    Topics: Amino Acid Sequence; Bacterial Outer Membrane Proteins; Biotin; Cysteine; Disulfides; Escherichia coli; Escherichia coli Proteins; Ferrichrome; Hot Temperature; Ligands; Maleimides; Molecular Sequence Data; Mutation; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Receptors, Virus; Sulfhydryl Reagents

1997
Determination of external loop topology in the serotonin transporter by site-directed chemical labeling.
    The Journal of biological chemistry, 1998, May-15, Volume: 273, Issue:20

    Topics: Biotin; Carrier Proteins; Cysteine; Lysine; Membrane Glycoproteins; Membrane Transport Proteins; Mutagenesis, Site-Directed; Nerve Tissue Proteins; Protein Conformation; Serotonin Plasma Membrane Transport Proteins; Succinimides

1998
Use of biotinylated-cysteinyl-tRNA as a non-RI probe in protein synthesis.
    Nucleic acids symposium series, 1997, Issue:37

    Topics: Biotin; Cell-Free System; Cysteine; Escherichia coli; Models, Molecular; Protein Biosynthesis; Protein Conformation; Proteins; RNA, Messenger; RNA, Transfer, Amino Acyl; RNA, Transfer, Cys

1997
The sensor regions of VDAC are translocated from within the membrane to the surface during the gating processes.
    Biophysical journal, 1998, Volume: 74, Issue:6

    Topics: Amino Acid Sequence; Amino Acid Substitution; Biotin; Cysteine; Electric Conductivity; Electrophysiology; Intracellular Membranes; Ion Channel Gating; Lipid Bilayers; Membrane Potentials; Membrane Proteins; Mitochondria; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Neurospora crassa; Porins; Protein Conformation; Protein Folding; Recombinant Proteins; Saccharomyces cerevisiae; Streptavidin; Voltage-Dependent Anion Channels

1998
The topology of VDAC as probed by biotin modification.
    The Journal of biological chemistry, 1998, Sep-18, Volume: 273, Issue:38

    Topics: Amino Acid Sequence; Binding Sites; Biotin; Biotinylation; Cysteine; Electrophysiology; Ion Channel Gating; Liposomes; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Neurospora crassa; Point Mutation; Porins; Protein Folding; Protein Structure, Secondary; Recombinant Proteins; Streptavidin; Voltage-Dependent Anion Channels

1998
Transmembrane topology mapping using biotin-containing sulfhydryl reagents.
    Methods in enzymology, 1998, Volume: 296

    Topics: Animals; Biotin; Biotinylation; Blotting, Western; Cell Membrane; Cells, Cultured; Cysteine; Electrophoresis, Polyacrylamide Gel; Indicators and Reagents; Membrane Proteins; Models, Molecular; Mutagenesis, Site-Directed; Protein Structure, Secondary; Recombinant Proteins; Sulfhydryl Reagents; Transfection

1998
Mapping the agonist binding site of the GABAA receptor: evidence for a beta-strand.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999, Jun-15, Volume: 19, Issue:12

    Topics: Animals; Binding Sites; Biotin; Chromosome Mapping; Cysteine; Dose-Response Relationship, Drug; Ethyl Methanesulfonate; GABA Agonists; gamma-Aminobutyric Acid; Humans; Indicators and Reagents; Kidney; Membrane Potentials; Models, Molecular; Molecular Sequence Data; Muscimol; Mutagenesis, Site-Directed; Oocytes; Protein Structure, Tertiary; Receptors, GABA-A; Sequence Homology, Amino Acid; Tritium; Xenopus

1999
Cysteine-scanning mutagenesis of an eukaryotic pore-forming toxin from sea anemone: topology in lipid membranes.
    European journal of biochemistry, 1999, Volume: 263, Issue:1

    Topics: 2-Naphthylamine; Amino Acid Sequence; Amino Acid Substitution; Animals; Avidin; Binding Sites; Biotin; Cloning, Molecular; Cnidarian Venoms; Cysteine; Liposomes; Membrane Lipids; Models, Molecular; Molecular Probes; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Sea Anemones; Solutions

1999
Cysteine-scanning mutagenesis of the periplasmic loop regions of PomA, a putative channel component of the sodium-driven flagellar motor in Vibrio alginolyticus.
    Journal of bacteriology, 2000, Volume: 182, Issue:4

    Topics: Amino Acid Sequence; Amino Acid Substitution; Bacterial Outer Membrane Proteins; Biotin; Cysteine; Dithiothreitol; Electroporation; Flagella; Maleimides; Molecular Sequence Data; Mutagenesis; Protein Structure, Secondary; Sodium; Vibrio

2000
Topography of diphtheria Toxin's T domain in the open channel state.
    The Journal of general physiology, 2000, Volume: 115, Issue:4

    Topics: Amino Acid Sequence; Amino Acid Substitution; Biotin; Cell Membrane; Cysteine; Diphtheria Toxin; Humans; Ion Channel Gating; Ion Channels; Lipid Bilayers; Maleimides; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Streptavidin

2000
Do cysteine 230 and lysine 238 of biotin carboxylase play a role in the activation of biotin?
    Biochemistry, 2000, Apr-11, Volume: 39, Issue:14

    Topics: Animals; Binding Sites; Biotin; Carbon-Nitrogen Ligases; Catalysis; Cysteine; Lysine; Mutagenesis, Site-Directed; Substrate Specificity

2000
A model for the topology of excitatory amino acid transporters determined by the extracellular accessibility of substituted cysteines.
    Neuron, 2000, Volume: 25, Issue:3

    Topics: Amino Acid Sequence; Amino Acid Transport System X-AG; Amino Acids; Animals; ATP-Binding Cassette Transporters; Biotin; COS Cells; Cysteine; Ethyl Methanesulfonate; Extracellular Space; Humans; Indicators and Reagents; Ion Channel Gating; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Tertiary

2000
Enzyme-mediated sulfide production for the reconstitution of [2Fe-2S] clusters into apo-biotin synthase of Escherichia coli. Sulfide transfer from cysteine to biotin.
    European journal of biochemistry, 2000, Volume: 267, Issue:9

    Topics: Bacterial Proteins; Biotin; Cysteine; Escherichia coli; Iron-Sulfur Proteins; Sulfides; Sulfurtransferases

2000
Simplification of complex peptide mixtures for proteomic analysis: reversible biotinylation of cysteinyl peptides.
    Electrophoresis, 2000, Volume: 21, Issue:9

    Topics: Animals; Biotin; Biotinylation; Cysteine; Humans; Mitochondria; Peptides; Proteome

2000
Identification of proteins containing cysteine residues that are sensitive to oxidation by hydrogen peroxide at neutral pH.
    Analytical biochemistry, 2000, Aug-01, Volume: 283, Issue:2

    Topics: Alkylation; Amino Acid Sequence; Animals; Biotin; Creatine Kinase; Cysteine; Ethylenediamines; Hydrogen Peroxide; Hydrogen-Ion Concentration; Molecular Sequence Data; Oxidation-Reduction; PC12 Cells; Proteins; Rabbits; Rats; Streptavidin

2000
Serine 121 is an essential amino acid for biotin sulfoxide reductase functionality.
    The Journal of biological chemistry, 2000, Nov-10, Volume: 275, Issue:45

    Topics: Alanine; Amino Acid Sequence; Amino Acids; Binding Sites; Biotin; Catalysis; Chromatography, High Pressure Liquid; Coenzymes; Conserved Sequence; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Factor Xa; Glutathione Transferase; Guanine Nucleotides; Iron-Sulfur Proteins; Ligands; Mass Spectrometry; Metalloproteins; Molecular Sequence Data; Molybdenum Cofactors; Mutagenesis, Site-Directed; NADP; Oxidative Stress; Oxidoreductases; Oxygen; Paraquat; Pteridines; Pterins; Recombinant Fusion Proteins; Rhodobacter sphaeroides; Sequence Homology, Amino Acid; Serine; Spectrometry, Fluorescence; Threonine; Time Factors

2000
Identification of oxidant-sensitive proteins: TNF-alpha induces protein glutathiolation.
    Biochemistry, 2000, Sep-12, Volume: 39, Issue:36

    Topics: Animals; Annexin A2; Biotin; Cattle; Cysteine; Dose-Response Relationship, Drug; Glutathione; Glyceraldehyde-3-Phosphate Dehydrogenases; HeLa Cells; Humans; Hydrogen Peroxide; Neoplasm Proteins; Oxidants; Peroxidases; Peroxiredoxin III; Peroxiredoxins; Proteins; Reactive Oxygen Species; Solubility; Succinimides; Tumor Necrosis Factor-alpha

2000
Structure and dynamics of the GABA binding pocket: A narrowing cleft that constricts during activation.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001, Jan-01, Volume: 21, Issue:1

    Topics: Animals; Binding Sites; Biotin; Cells, Cultured; Cysteine; Dose-Response Relationship, Drug; GABA Agonists; GABA Antagonists; GABA Modulators; gamma-Aminobutyric Acid; Ion Channel Gating; Ligands; Mutagenesis, Site-Directed; Oocytes; Patch-Clamp Techniques; Protein Structure, Tertiary; Rats; Receptors, GABA-A; Structure-Activity Relationship; Sulfhydryl Reagents

2001
Immobilization of sugar-non-specific nucleases by utilizing the streptavidin--biotin interaction.
    Journal of biotechnology, 2001, May-04, Volume: 87, Issue:2

    Topics: Amino Acid Sequence; Base Sequence; Biotin; Carbohydrate Metabolism; Chromatography, Gel; Codon; Cysteine; Endodeoxyribonucleases; Endonucleases; Endoribonucleases; Enzymes, Immobilized; Molecular Sequence Data; Phosphoric Diester Hydrolases; Protein Engineering; Receptors, Growth Factor; Streptavidin; Surface Plasmon Resonance; Ultrafiltration

2001
Quantitative analysis of bacterial and mammalian proteomes using a combination of cysteine affinity tags and 15N-metabolic labeling.
    Analytical chemistry, 2001, May-01, Volume: 73, Issue:9

    Topics: Animals; Avidin; Bacteria; Biotin; Cells, Cultured; Chromatography, Affinity; Chromatography, Liquid; Cysteine; Melanoma, Experimental; Mice; Nitrogen Isotopes; Peptide Mapping; Peptides; Proteome; Spectrometry, Mass, Electrospray Ionization; Spectroscopy, Fourier Transform Infrared; Trypsin

2001
Membrane immunoglobulins are stabilized by interchain disulfide bonds occurring within the extracellular membrane-proximal domain.
    Biochemistry, 2001, Sep-04, Volume: 40, Issue:35

    Topics: Amino Acid Sequence; Biotin; Cysteine; Disulfides; Humans; Molecular Sequence Data; Mutation; Receptors, Antigen, B-Cell; Sequence Alignment

2001
Transmembrane topology of the sulfonylurea receptor SUR1.
    The Journal of biological chemistry, 2001, Nov-02, Volume: 276, Issue:44

    Topics: Animals; ATP-Binding Cassette Transporters; Biotin; COS Cells; Cysteine; Models, Molecular; Mutagenesis; Patch-Clamp Techniques; Potassium Channels; Potassium Channels, Inwardly Rectifying; Receptors, Drug; Sulfonylurea Receptors

2001
Cysteine-directed cross-linking localizes regions of the human erythrocyte anion-exchange protein (AE1) relative to the dimeric interface.
    The Biochemical journal, 2001, Nov-01, Volume: 359, Issue:Pt 3

    Topics: Amino Acid Sequence; Anion Exchange Protein 1, Erythrocyte; Biotin; Cell Line; Cross-Linking Reagents; Cysteine; Dimerization; Humans; Maleimides; Molecular Sequence Data; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Transfection

2001
Structure-function studies of Escherichia coli biotin synthase via a chemical modification and site-directed mutagenesis approach.
    Journal of biochemistry, 2001, Volume: 130, Issue:5

    Topics: Amino Acid Sequence; Binding Sites; Biotin; Cysteine; Dimerization; Enzyme Activation; Escherichia coli; Iron-Sulfur Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Sequence Homology, Amino Acid; Structure-Activity Relationship; Sulfurtransferases

2001
The biotin switch method for the detection of S-nitrosylated proteins.
    Science's STKE : signal transduction knowledge environment, 2001, Jun-12, Volume: 2001, Issue:86

    Topics: Animals; Biotin; Biotinylation; Cysteine; False Positive Reactions; Humans; Immunoblotting; Proteins; S-Nitrosothiols; Signal Transduction

2001
Conserved cysteine residues in the extracellular loop of the human P2X(1) receptor form disulfide bonds and are involved in receptor trafficking to the cell surface.
    Molecular pharmacology, 2002, Volume: 61, Issue:2

    Topics: Adenosine Triphosphate; Alanine; Amino Acid Sequence; Amino Acid Substitution; Animals; Biological Transport; Biotin; Biotinylation; Conserved Sequence; Cysteine; Disulfides; Dithiothreitol; Electrophysiology; Humans; Membrane Proteins; Mercaptoethanol; Molecular Sequence Data; Mutation; Oocytes; Protein Structure, Tertiary; Receptors, Purinergic P2; Receptors, Purinergic P2X; Reducing Agents; Sequence Homology, Amino Acid; Xenopus laevis

2002
Reductive cleavage of S-adenosylmethionine by biotin synthase from Escherichia coli.
    The Journal of biological chemistry, 2002, Apr-19, Volume: 277, Issue:16

    Topics: Biotin; Cysteine; Electron Spin Resonance Spectroscopy; Escherichia coli; Kinetics; Methionine; Models, Chemical; Mutation; S-Adenosylmethionine; Spectroscopy, Mossbauer; Sulfurtransferases; Time Factors

2002
The iron-sulfur center of biotin synthase: site-directed mutants.
    Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 2002, Volume: 7, Issue:1-2

    Topics: Acetyltransferases; Amino Acid Motifs; Amino Acid Substitution; Biotin; Cysteine; Electron Spin Resonance Spectroscopy; Enzymes; Escherichia coli; Intramolecular Transferases; Iron; Iron Chelating Agents; Iron-Sulfur Proteins; Mutagenesis, Site-Directed; Oxidation-Reduction; Sulfurtransferases

2002
S-thiolation of HSP27 regulates its multimeric aggregate size independently of phosphorylation.
    The Journal of biological chemistry, 2002, Jun-14, Volume: 277, Issue:24

    Topics: Animals; Biotin; Biotinylation; Blotting, Western; Cysteine; Enzyme Inhibitors; Heart; Heat-Shock Proteins; HSP27 Heat-Shock Proteins; Hydrogen Peroxide; Male; Neoplasm Proteins; Oxidative Stress; Perfusion; Phosphorylation; Rats; Rats, Wistar; Serine; Sulfhydryl Compounds; Time Factors

2002
Cysteine-scanning mutagenesis reveals a conformationally sensitive reentrant pore-loop in the glutamate transporter GLT-1.
    The Journal of biological chemistry, 2002, Jul-19, Volume: 277, Issue:29

    Topics: Amino Acid Substitution; Biotin; Cysteine; Excitatory Amino Acid Transporter 2; Glutamic Acid; HeLa Cells; Humans; Indicators and Reagents; Kainic Acid; Models, Molecular; Mutagenesis, Site-Directed; Protein Conformation; Protein Structure, Secondary; Sodium; Structure-Activity Relationship

2002
Peroxynitrite inactivates the human dopamine transporter by modification of cysteine 342: potential mechanism of neurotoxicity in dopamine neurons.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 2002, Jun-01, Volume: 22, Issue:11

    Topics: Antioxidants; Biological Transport; Biotin; Cell Line; Cysteine; Dopamine; Dopamine Plasma Membrane Transport Proteins; Dose-Response Relationship, Drug; Free Radical Scavengers; Humans; Kidney; Membrane Glycoproteins; Membrane Transport Modulators; Membrane Transport Proteins; Mutagenesis, Site-Directed; Nerve Tissue Proteins; Neurons; Neurotoxicity Syndromes; Peroxynitrous Acid; Structure-Activity Relationship; Substrate Specificity; Sulfhydryl Reagents; Transfection

2002
Disulfide exchange in domain 2 of CD4 is required for entry of HIV-1.
    Nature immunology, 2002, Volume: 3, Issue:8

    Topics: Arsenicals; Biotin; CD4 Antigens; CD4-Positive T-Lymphocytes; Cysteine; Glutathione; HIV-1; Humans; Immunoblotting; Lysine; Maleimides; Mutation; Oxidation-Reduction; Protein Structure, Tertiary; Receptors, HIV; Succinimides; Sulfhydryl Compounds; Thioredoxins

2002
Natural supramolecular building blocks. Cysteine-added mutants of cowpea mosaic virus.
    Chemistry & biology, 2002, Volume: 9, Issue:7

    Topics: Avidin; Binding Sites; Biotin; Capsid; Chromatography, Liquid; Comovirus; Cysteine; Electrophoresis, Polyacrylamide Gel; Fluorescein; Gold Colloid; Microscopy, Electron; Models, Molecular; Mutagenesis, Insertional; Nanotechnology; Protein Subunits; Reverse Transcriptase Polymerase Chain Reaction; Rhodamines; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds

2002
A novel synthesis of (+)-biotin from L-cysteine.
    The Journal of organic chemistry, 2002, Aug-09, Volume: 67, Issue:16

    Topics: Biotin; Cysteine; Indicators and Reagents; Models, Molecular; Molecular Conformation; Stereoisomerism

2002
Disulfide bond switches.
    Nature biotechnology, 2002, Volume: 20, Issue:9

    Topics: Arsenicals; Biotin; CD4 Antigens; CD4-Positive T-Lymphocytes; Cysteine; Disulfides; Glutathione; HIV-1; Humans; Immunoblotting; Lysine; Maleimides; Mutation; Oxidation-Reduction; Protein Structure, Tertiary; Receptors, HIV; Succinimides; Sulfhydryl Compounds; Thioredoxins

2002
Functional immobilization of a ligand-activated G-protein-coupled receptor.
    Chembiochem : a European journal of chemical biology, 2002, Oct-04, Volume: 3, Issue:10

    Topics: Animals; Antibodies, Monoclonal; Avidin; Biotin; Cells, Cultured; Cysteine; Epitopes; Fluoresceins; GTP-Binding Proteins; Humans; Insecta; Kinetics; Ligands; Microscopy, Fluorescence; Protein Conformation; Receptors, Adrenergic, beta-2; Substrate Specificity; Surface Plasmon Resonance

2002
Enhancing the thermal stability of avidin. Introduction of disulfide bridges between subunit interfaces.
    The Journal of biological chemistry, 2003, Jan-24, Volume: 278, Issue:4

    Topics: Animals; Avidin; Biotin; Calorimetry; Cell Line; Chickens; Cysteine; Disulfides; DNA, Complementary; Dose-Response Relationship, Drug; Insecta; Isoleucine; Models, Molecular; Mutation; Protein Binding; Protein Conformation; Protein Denaturation; Temperature; Time Factors

2003
A ribozyme with michaelase activity: synthesis of the substrate precursors.
    Bioorganic & medicinal chemistry, 2003, Jan-17, Volume: 11, Issue:2

    Topics: Amides; Biotin; Cysteine; Gene Library; Guanosine; Kinetics; Oligonucleotides; Phosphorus Isotopes; Photochemistry; Reverse Transcriptase Polymerase Chain Reaction; RNA, Catalytic; Substrate Specificity; Sulfhydryl Compounds; Templates, Genetic; Thymidylate Synthase

2003
Diazotrifluoropropionamido-containing prenylcysteines: syntheses and applications for studying isoprenoid-protein interactions.
    Organic letters, 2003, Mar-06, Volume: 5, Issue:5

    Topics: Amides; Autoradiography; Benzophenones; Binding Sites; Binding, Competitive; Biotin; Cross-Linking Reagents; Cysteine; Diazonium Compounds; Guanine Nucleotide Dissociation Inhibitors; Photoaffinity Labels; Photolysis; Protein Prenylation; rho-Specific Guanine Nucleotide Dissociation Inhibitors; Streptavidin

2003
Proteomic analysis of S-nitrosylated proteins in mesangial cells.
    Molecular & cellular proteomics : MCP, 2003, Volume: 2, Issue:3

    Topics: Animals; Biotin; Cell Line; Cysteine; Electrophoresis, Gel, Two-Dimensional; Glomerular Mesangium; Mice; Nitric Oxide; Nitric Oxide Donors; Nitric Oxide Synthase Type II; Proteome; S-Nitrosoglutathione; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

2003
Monitoring the cellular surface display of recombinant proteins by cysteine labeling and flow cytometry.
    Chembiochem : a European journal of chemical biology, 2003, May-09, Volume: 4, Issue:5

    Topics: Adrenodoxin; Amino Acid Sequence; Animals; Bacterial Outer Membrane Proteins; Base Sequence; Biotin; Cattle; Cysteine; Escherichia coli; Escherichia coli Proteins; Flow Cytometry; Fluorescein; Maleimides; Models, Molecular; Molecular Sequence Data; Protein Transport; Recombinant Fusion Proteins; Staining and Labeling

2003
Semisynthesis and characterization of the first analogues of pro-neuropeptide y.
    Chembiochem : a European journal of chemical biology, 2003, May-09, Volume: 4, Issue:5

    Topics: Amino Acid Sequence; Biotin; Cysteine; Escherichia coli; Esters; Fluoresceins; Molecular Sequence Data; Neuropeptide Y; Peptide Fragments; Proline; Protein Precursors; Protein Splicing; Recombinant Fusion Proteins; Staining and Labeling; Sulfhydryl Compounds

2003
Detection of cysteine S-nitrosylation and tyrosine 3-nitration in kidney proteins.
    Methods in molecular medicine, 2003, Volume: 86

    Topics: Animals; Biotin; Blotting, Western; Chromatography, High Pressure Liquid; Cysteine; Electrochemistry; In Vitro Techniques; Kidney; Mice; Nitric Oxide; Proteins; Rats; Tyrosine

2003
The region between transmembrane domains 1 and 2 of the reduced folate carrier forms part of the substrate-binding pocket.
    The Journal of biological chemistry, 2003, Oct-17, Volume: 278, Issue:42

    Topics: Alanine; Amino Acid Sequence; Animals; Bacterial Proteins; Biotin; Biotinylation; Carrier Proteins; Cell Membrane; Cricetinae; Cysteine; Cytosol; Folic Acid; Green Fluorescent Proteins; Kinetics; Luminescent Proteins; Membrane Transport Proteins; Methotrexate; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Recombinant Fusion Proteins; Reduced Folate Carrier Protein; RNA; Streptolysins

2003
POLYSACCHARIDE STORAGE AND GROWTH EFFICIENCY IN RUMINOCOCCUS ALBUS.
    Journal of bacteriology, 1963, Volume: 86

    Topics: 4-Aminobenzoic Acid; Animals; Bacteria; Benzoates; Biotin; Butyrates; Cellobiose; Culture Media; Cysteine; Disaccharides; Fatty Acids; Fermentation; Polysaccharides; Polysaccharides, Bacterial; Pyridoxine; Research; Rumen; Ruminococcus; Sulfides

1963
STUDIES ON THE FORMATION OF BIOTIN FROM DESTHIOBIOTIN AND SULFATE IN SACCHAROMYCES CEREVISIAE. I. ON BIOTIN FORMATION FROM DESTHIOBIOTIN BY WASHED CELL SUSPENSION OF YEAST.
    The Journal of vitaminology, 1964, Sep-10, Volume: 10

    Topics: Biotin; Cysteine; Glucose; Glutathione; Homocysteine; Metabolism; Methionine; Pharmacology; Research; Saccharomyces; Saccharomyces cerevisiae; Sulfates; Sulfides; Sulfur Oxides; Yeast, Dried

1964
STUDIES ON THE FORMATION OF BIOTIN FROM DESTHIOBIOTIN AND SULFATE IN SACCHAROMYCES CEREVISIAE. II. ON SULFUR SOURCES OF BIOTIN FORMATION BY WASHED CELL SUSPENSION OF YEAST.
    The Journal of vitaminology, 1964, Sep-10, Volume: 10

    Topics: Biotin; Choline; Culture Media; Cysteine; Cystine; Ethionine; Glucose; Glutathione; Homocysteine; Metabolism; Methionine; Research; Saccharomyces; Saccharomyces cerevisiae; Sulfates; Sulfur; Sulfur Oxides; Yeast, Dried

1964
STUDIES ON THE FORMATION OF BIOTIN FROM DESTHIOBIOTIN AND SULFATE IN SACCHAROMYCES CEREVISIAE. 3. ISOTOPIC STUDIES ON BIOTIN FORMATION FROM DESTHIOBIOTIN AND S35-LABELLED SULFATE BY WASHED CELL SUSPENSION OF YEAST.
    The Journal of vitaminology, 1964, Sep-10, Volume: 10

    Topics: Biotin; Chromatography; Culture Media; Cysteine; Cystine; Glucose; Metabolism; Methionine; Neurospora; Research; Saccharomyces; Saccharomyces cerevisiae; Sulfates; Yeast, Dried

1964
Procollagen VII self-assembly depends on site-specific interactions and is promoted by cleavage of the NC2 domain with procollagen C-proteinase.
    Biochemistry, 2003, Oct-07, Volume: 42, Issue:39

    Topics: Amino Acid Sequence; Animals; Binding Sites; Biotin; Bone Morphogenetic Protein 1; Bone Morphogenetic Proteins; Cloning, Molecular; Collagen Type VII; Colorimetry; Cysteine; Dimerization; Disulfides; Humans; Metalloendopeptidases; Mice; Microscopy, Electron; Models, Molecular; Molecular Sequence Data; Protein Binding; Protein Structure, Secondary; Protein Structure, Tertiary; Recombinant Proteins; Sequence Deletion

2003
Mislocalization of membrane proteins associated with multidrug resistance in cisplatin-resistant cancer cell lines.
    Cancer research, 2003, Sep-15, Volume: 63, Issue:18

    Topics: Antineoplastic Agents; ATP Binding Cassette Transporter, Subfamily B, Member 1; Biotin; Carbon Radioisotopes; Carboplatin; Carrier Proteins; Cell Membrane; Cisplatin; Cysteine; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Endoplasmic Reticulum Chaperone BiP; Folate Receptors, GPI-Anchored; HeLa Cells; Humans; KB Cells; Lysosomes; Methionine; Methotrexate; Receptors, Cell Surface; Succinimides; Sulfur Radioisotopes; Triiodobenzoic Acids; Tritium

2003
S-Nitrosation regulates the activation of endogenous procaspase-9 in HT-29 human colon carcinoma cells.
    The Journal of biological chemistry, 2004, Mar-12, Volume: 279, Issue:11

    Topics: Apoptosis; Ascorbic Acid; Biotin; Blotting, Western; Caspase 3; Caspase 8; Caspase 9; Caspases; Cell Death; Colonic Neoplasms; Cysteine; DNA; Enzyme Activation; Enzyme Inhibitors; Enzyme-Linked Immunosorbent Assay; HT29 Cells; Humans; Interferon-gamma; Models, Biological; Nitric Oxide; Nitric Oxide Synthase; Nitrosation; Precipitin Tests; Time Factors; Tumor Necrosis Factor-alpha

2004
A microdomain formed by the extracellular ends of the transmembrane domains promotes activation of the G protein-coupled alpha-factor receptor.
    Molecular and cellular biology, 2004, Volume: 24, Issue:5

    Topics: Amino Acid Sequence; Binding Sites; Biotin; Cell Division; Cysteine; Genes, Reporter; Ligands; Mesylates; Models, Molecular; Mutation; Phenotype; Pheromones; Protein Binding; Protein Structure, Secondary; Receptors, G-Protein-Coupled; Receptors, Mating Factor; Receptors, Peptide; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Signal Transduction; Transcription Factors

2004
Site-specific immobilization of biotinylated proteins for protein microarray analysis.
    Methods in molecular biology (Clifton, N.J.), 2004, Volume: 264

    Topics: Avidin; Base Sequence; Biotin; Cysteine; Genetic Vectors; Humans; Molecular Sequence Data; Molecular Structure; Protein Array Analysis; Proteins; Recombinant Fusion Proteins

2004
Site-specific peptide immobilization strategies for the rapid detection of kinase activity on microarrays.
    Methods in molecular biology (Clifton, N.J.), 2004, Volume: 264

    Topics: Antibodies; Avidin; Biotin; Cysteine; Esters; Fluorescent Dyes; Molecular Structure; Peptides; Polyethylene Glycols; Protein Array Analysis; Protein Kinases

2004
Membrane topology of system xc- light subunit reveals a re-entrant loop with substrate-restricted accessibility.
    The Journal of biological chemistry, 2004, Jul-23, Volume: 279, Issue:30

    Topics: Amino Acid Transport System y+; Amino Acid Transport Systems; Binding Sites; Biotin; Cell Membrane; Cysteine; HeLa Cells; Humans; Kinetics; Models, Molecular; Mutagenesis, Site-Directed; Protein Subunits; Recombinant Proteins

2004
Identification of oxidized mitochondrial proteins in alcohol-exposed human hepatoma cells and mouse liver.
    Proteomics, 2004, Volume: 4, Issue:11

    Topics: Alcohol Drinking; Animals; Bacterial Proteins; Biotin; Carcinoma, Hepatocellular; Cysteine; Electrophoresis, Gel, Two-Dimensional; Ethanol; Humans; Liver; Mice; Mitochondria; Mitochondrial Proteins; Oxidation-Reduction; Sepharose; Sequence Analysis, Protein

2004
A practical synthesis of (+)-biotin from L-cysteine.
    Chemistry (Weinheim an der Bergstrasse, Germany), 2004, Nov-19, Volume: 10, Issue:23

    Topics: Biotin; Cysteine; Molecular Structure; Vitamin B Complex

2004
Electrospray mass spectrometry of a series of mixed nitrido 99gTc- heterocomplexes conjugated with bioactive molecules.
    European journal of mass spectrometry (Chichester, England), 2004, Volume: 10, Issue:5

    Topics: Biotin; Cysteine; Nitrogen Compounds; Piperazine; Piperazines; Radioisotopes; Spectrometry, Mass, Electrospray Ionization; Technetium; Technetium Compounds

2004
Mutation of glutamate 155 of the GABAA receptor beta2 subunit produces a spontaneously open channel: a trigger for channel activation.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004, Dec-15, Volume: 24, Issue:50

    Topics: Animals; Binding Sites; Biotin; Cysteine; GABA-A Receptor Agonists; Glutamic Acid; Ion Channel Gating; Kinetics; Ligands; Mutagenesis, Site-Directed; Mutation; Patch-Clamp Techniques; Pentobarbital; Protein Conformation; Rats; Receptors, GABA-A; Recombinant Proteins; Xenopus laevis

2004
Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits.
    The Journal of biological chemistry, 2005, Apr-01, Volume: 280, Issue:13

    Topics: 3',5'-Cyclic-GMP Phosphodiesterases; Animals; Biotin; Blotting, Western; Catalysis; Catalytic Domain; Cattle; Cross-Linking Reagents; Cyclic Nucleotide Phosphodiesterases, Type 6; Cysteine; Dimerization; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Eye Proteins; Light; Mass Spectrometry; Models, Biological; Models, Chemical; Peptides; Phosphoric Diester Hydrolases; Protein Binding; Protein Structure, Tertiary; Retinal Rod Photoreceptor Cells; Serine; Ultraviolet Rays; Vision, Ocular

2005
Selective detection of 2-deoxyribonolactone in DNA.
    Journal of the American Chemical Society, 2005, Mar-09, Volume: 127, Issue:9

    Topics: Autoradiography; Biotin; Cysteine; DNA; DNA Damage; Gas Chromatography-Mass Spectrometry; Horseradish Peroxidase; Oxidation-Reduction; Sensitivity and Specificity; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sugar Acids

2005
Characterization and application of the biotin-switch assay for the identification of S-nitrosated proteins.
    Free radical biology & medicine, 2005, Apr-01, Volume: 38, Issue:7

    Topics: Animals; Ascorbic Acid; Biotin; Cells, Cultured; Cysteine; Mice; Nitrosation; Oxidation-Reduction; Proteins; Proteomics; S-Nitrosothiols

2005
A pulsed potential waveform displaying enhanced detection capabilities towards sulfur-containing compounds at a gold working electrode.
    Journal of chromatography. A, 2005, Feb-25, Volume: 1066, Issue:1-2

    Topics: Acetylcysteine; Biotin; Chromatography, High Pressure Liquid; Chromatography, Ion Exchange; Cysteine; Cystine; Electrochemistry; Electrodes; Glutathione; Gold; Homocysteine; Methionine; Oxidation-Reduction; Reproducibility of Results; Sensitivity and Specificity; Sulfur Compounds; Thioctic Acid

2005
S-nitrosylation of Hsp90 promotes the inhibition of its ATPase and endothelial nitric oxide synthase regulatory activities.
    Proceedings of the National Academy of Sciences of the United States of America, 2005, Jun-14, Volume: 102, Issue:24

    Topics: Adenosine Triphosphatases; Amino Acid Sequence; Biotin; Cysteine; Endothelial Cells; HSP90 Heat-Shock Proteins; Humans; Luminescent Measurements; Mass Spectrometry; Models, Molecular; Molecular Sequence Data; Nitric Oxide Synthase; Nitric Oxide Synthase Type III; Ozone; Protein Processing, Post-Translational; S-Nitrosothiols; Signal Transduction

2005
Specific patterns of electrophile adduction trigger Keap1 ubiquitination and Nrf2 activation.
    The Journal of biological chemistry, 2005, Sep-09, Volume: 280, Issue:36

    Topics: Biotin; Cell Cycle Proteins; Cell Line; Cullin Proteins; Cysteine; DNA-Binding Proteins; Electrophoresis, Polyacrylamide Gel; Humans; Intracellular Signaling Peptides and Proteins; Kelch-Like ECH-Associated Protein 1; Mass Spectrometry; NF-E2-Related Factor 2; Peptides; Proteins; Trans-Activators; Transfection; Ubiquitin

2005
Transforming activity of the Rho family GTPase, Wrch-1, a Wnt-regulated Cdc42 homolog, is dependent on a novel carboxyl-terminal palmitoylation motif.
    The Journal of biological chemistry, 2005, Sep-23, Volume: 280, Issue:38

    Topics: Amino Acid Motifs; Amino Acid Sequence; Animals; Biotin; Blotting, Western; cdc42 GTP-Binding Protein; Cell Adhesion; Cell Membrane; Cell Proliferation; Cysteine; Cytosol; Endosomes; Esters; Green Fluorescent Proteins; Mice; Microscopy, Fluorescence; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; NIH 3T3 Cells; Palmitic Acid; Protein Binding; Protein Structure, Tertiary; Recombinant Proteins; rho GTP-Binding Proteins; Sequence Homology, Amino Acid; Signal Transduction; Transfection

2005
An optimized strategy for ICAT quantification of membrane proteins.
    Molecular & cellular proteomics : MCP, 2006, Volume: 5, Issue:1

    Topics: Affinity Labels; Animals; Biotin; Cattle; Chickens; Cysteine; Electrophoresis, Gel, Two-Dimensional; Embryo, Mammalian; Embryo, Nonmammalian; Isotope Labeling; Lactoglobulins; Membrane Proteins; Mice; Ovalbumin; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stem Cells; Trypsin; Urea

2006
Inducible nitric oxide synthase binds, S-nitrosylates, and activates cyclooxygenase-2.
    Science (New York, N.Y.), 2005, Dec-23, Volume: 310, Issue:5756

    Topics: Animals; Biotin; Catalysis; Cell Line; Cyclooxygenase 2; Cysteine; Dinoprostone; Enzyme Activation; Humans; Mice; Nitric Oxide Donors; Nitric Oxide Synthase Type II; Nitroso Compounds; Protein Binding; S-Nitrosoglutathione

2005
Cytosolic and nuclear protein targets of thiol-reactive electrophiles.
    Chemical research in toxicology, 2006, Volume: 19, Issue:1

    Topics: Alkylation; Amino Acid Sequence; Biotin; Cell Line; Cell-Free System; Cysteine; Cytosol; Humans; Nuclear Proteins; Peptide Fragments; Peptide Mapping; Protein Binding; Protein Conformation; Proteins; Proteomics; Sulfhydryl Compounds

2006
Inhibition of protein phosphatase 2A activity by selective electrophile alkylation damage.
    Biochemistry, 2006, Aug-22, Volume: 45, Issue:33

    Topics: Alkylation; Biotin; Blotting, Western; Catalysis; Cell Line; Cysteine; Enzyme Activation; Enzyme Inhibitors; Holoenzymes; Humans; Phosphoprotein Phosphatases; Phosphorylation; Protein Conformation; Protein Phosphatase 2; Signal Transduction; Sulfhydryl Compounds

2006
Plant viral capsids as nanobuilding blocks: construction of arrays on solid supports.
    Langmuir : the ACS journal of surfaces and colloids, 2006, Nov-21, Volume: 22, Issue:24

    Topics: Biotin; Biotinylation; Capsid; Cysteine; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Gold; Models, Chemical; Molecular Conformation; Nanoparticles; Nanostructures; Nanotechnology; Plant Viruses; Protein Array Analysis; Spectrometry, Fluorescence

2006
Design and characterization of an active site selective caspase-3 transnitrosating agent.
    ACS chemical biology, 2006, Nov-21, Volume: 1, Issue:10

    Topics: Apoptosis; Binding Sites; Biotin; Caspase 3; Caspase 9; Chemistry, Pharmaceutical; Cyclic GMP; Cysteine; Drug Design; Enzyme Activation; Humans; Kinetics; Nitric Oxide; Nitrosation; Peptides

2006
Inorganic mercury interacts with cysteine residues (C451 and C474) of hOCT2 to reduce its transport activity.
    American journal of physiology. Renal physiology, 2007, Volume: 292, Issue:5

    Topics: Animals; Biological Transport; Biotin; CHO Cells; Cricetinae; Cricetulus; Cysteine; Humans; Kinetics; Mercuric Chloride; Organic Cation Transport Proteins; Organic Cation Transporter 2; Substrate Specificity; Tetraethylammonium

2007
Assessment and application of the biotin switch technique for examining protein S-nitrosylation under conditions of pharmacologically induced oxidative stress.
    The Journal of biological chemistry, 2007, May-11, Volume: 282, Issue:19

    Topics: Ascorbic Acid; Biotin; Cells, Cultured; Cysteine; Glutathione; Humans; Kidney; NG-Nitroarginine Methyl Ester; Nitric Oxide; Nitric Oxide Synthase; Nitrogen; Oxidation-Reduction; Oxidative Stress; Photolysis; Protein Tyrosine Phosphatase, Non-Receptor Type 1; Protein Tyrosine Phosphatases; Sulfhydryl Compounds; Sunlight

2007
Evolution of an interloop disulfide bond in high-affinity antibody mimics based on fibronectin type III domain and selected by yeast surface display: molecular convergence with single-domain camelid and shark antibodies.
    Journal of molecular biology, 2007, May-11, Volume: 368, Issue:4

    Topics: Amino Acid Sequence; Animals; Antibodies; Biomimetics; Biotin; Camelids, New World; Cysteine; Disulfides; Fibronectins; Humans; Models, Molecular; Molecular Sequence Data; Muramidase; Mutation; Peptide Library; Protein Structure, Tertiary; RNA, Messenger; Saccharomyces cerevisiae; Sharks

2007
Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays.
    Nature protocols, 2006, Volume: 1, Issue:5

    Topics: Biotin; Cell-Free System; Cysteine; Protein Array Analysis; Proteins

2006
Analysis of protein adduction kinetics by quantitative mass spectrometry: competing adduction reactions of glutathione-S-transferase P1-1 with electrophiles.
    Chemico-biological interactions, 2007, Jun-30, Volume: 168, Issue:2

    Topics: Biotin; Cysteine; Glutathione S-Transferase pi; Isocyanates; Kinetics; Naphthalenesulfonates; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry

2007
Protein targets of reactive electrophiles in human liver microsomes.
    Chemical research in toxicology, 2007, Volume: 20, Issue:6

    Topics: Acetaminophen; Alkylating Agents; Biotin; Blotting, Western; Calreticulin; Cell Line; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Cysteine; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Glutathione Transferase; Humans; Iodoacetamide; Microsomes, Liver; Molecular Structure; Protein Binding; Proteins; Tandem Mass Spectrometry

2007
Identification of arsenic-binding proteins in human breast cancer cells.
    Cancer letters, 2007, Sep-18, Volume: 255, Issue:1

    Topics: Antineoplastic Agents; Arsenic; Biotin; Breast Neoplasms; Carrier Proteins; Cell Line, Tumor; Cysteine; Humans; Models, Chemical; Models, Molecular; Molecular Conformation; Protein Binding; Pyruvate Kinase; Tubulin

2007
Outward stabilization of the S4 segments in domains III and IV enhances lidocaine block of sodium channels.
    The Journal of physiology, 2007, Jul-01, Volume: 582, Issue:Pt 1

    Topics: Anesthetics, Local; Anti-Arrhythmia Agents; Arginine; Binding Sites; Biotin; Cell Line; Cysteine; Dose-Response Relationship, Drug; Humans; Ion Channel Gating; Kinetics; Lidocaine; Membrane Potentials; Muscle Proteins; Mutation; NAV1.5 Voltage-Gated Sodium Channel; Protein Conformation; Protein Structure, Tertiary; Sodium Channel Blockers; Sodium Channels; Transfection

2007
Setting up for the block: the mechanism underlying lidocaine's use-dependent inhibition of sodium channels.
    The Journal of physiology, 2007, Jul-01, Volume: 582, Issue:Pt 1

    Topics: Anesthetics, Local; Anti-Arrhythmia Agents; Arginine; Binding Sites; Biotin; Cell Line; Cysteine; Humans; Ion Channel Gating; Kinetics; Lidocaine; Membrane Potentials; Muscle Proteins; Mutation; NAV1.5 Voltage-Gated Sodium Channel; Protein Conformation; Protein Structure, Tertiary; Sodium Channel Blockers; Sodium Channels; Transfection

2007
Avidin-biotin system: a small library of cysteine biotinylated derivatives designed for the [99mTc(N)(PNP)]2+ metal fragment.
    Nuclear medicine and biology, 2007, Volume: 34, Issue:5

    Topics: Animals; Avidin; Biotin; Biotinylation; Cysteine; Drug Design; Drug Stability; Humans; Male; Metabolic Clearance Rate; Metals; Mice; Mice, Inbred BALB C; Organ Specificity; Peptide Library; Radiopharmaceuticals; Rats; Technetium; Tissue Distribution

2007
Unbiased identification of cysteine S-nitrosylation sites on proteins.
    Nature protocols, 2007, Volume: 2, Issue:7

    Topics: Animals; Ascorbic Acid; Biotin; Cysteine; Mice; Nerve Tissue Proteins; Nitric Oxide; Nitric Oxide Synthase; Nitroso Compounds; Proteome; Proteomics; Trypsin

2007
The natural product avrainvillamide binds to the oncoprotein nucleophosmin.
    Journal of the American Chemical Society, 2007, Nov-21, Volume: 129, Issue:46

    Topics: Alanine; Animals; Antineoplastic Agents; Binding Sites; Biological Products; Biotin; Blotting, Western; Chlorocebus aethiops; COS Cells; Cysteine; HeLa Cells; Humans; Indoles; Microscopy, Fluorescence; Nuclear Proteins; Nucleophosmin; Oncogene Proteins; Tumor Cells, Cultured

2007
Sites of alkylation of human Keap1 by natural chemoprevention agents.
    Journal of the American Society for Mass Spectrometry, 2007, Volume: 18, Issue:12

    Topics: Alkylation; Amino Acid Sequence; Biological Products; Biotin; Chemoprevention; Chromatography, Liquid; Cysteine; Humans; Intracellular Signaling Peptides and Proteins; Kelch-Like ECH-Associated Protein 1; Mass Spectrometry; Molecular Sequence Data; Peptides; Protein Processing, Post-Translational

2007
Anti-parallel membrane topology of a homo-dimeric multidrug transporter, EmrE.
    Journal of biochemistry, 2007, Volume: 142, Issue:5

    Topics: Antiporters; Biological Transport; Biotin; Cell Membrane Permeability; Cysteine; Cytoplasm; Dimerization; Escherichia coli Proteins; Maleimides; Membrane Transport Proteins; Multidrug Resistance-Associated Proteins; Mutation; Periplasm; Polyethylene; Proline; Protein Structure, Secondary; Threonine

2007
Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins.
    Free radical biology & medicine, 2008, Apr-01, Volume: 44, Issue:7

    Topics: Biochemistry; Biotin; Bronchi; Carbocyanines; Copper; Cysteine; Epithelial Cells; Free Radicals; Hemoglobins; Humans; Models, Biological; Nitric Oxide; Nitrogen; S-Nitrosothiols; Sulfhydryl Compounds

2008
Rapid identification of reactive cysteine residues for site-specific labeling of antibody-Fabs.
    Journal of immunological methods, 2008, Mar-20, Volume: 332, Issue:1-2

    Topics: Antibodies, Monoclonal; Antigen-Antibody Reactions; Binding Sites; Biotin; Cell Line, Tumor; Chromatography, Liquid; Cysteine; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Gene Expression Regulation; Genetic Engineering; Genetic Variation; Humans; Immunoglobulin Fab Fragments; Maleimides; Mass Spectrometry; Models, Molecular; Mutagenesis, Site-Directed; Plasmids; Sensitivity and Specificity; Staining and Labeling; Surface Properties

2008
On-gel fluorescent visualization and the site identification of S-nitrosylated proteins.
    Analytical biochemistry, 2008, Jun-15, Volume: 377, Issue:2

    Topics: Acetic Acid; Animals; Binding Sites; Biotin; Cattle; Chromatography, Liquid; Cysteine; Fluorescence; Gels; Nitric Oxide; Proteins; Serum Albumin, Bovine; Staining and Labeling; Tandem Mass Spectrometry; Time Factors

2008
Mechanism for the cholesterol-lowering action of egg white protein in rats.
    Bioscience, biotechnology, and biochemistry, 2008, Volume: 72, Issue:6

    Topics: Animals; Biotin; Cholesterol; Cysteine; Egg Proteins; Feces; Intestinal Mucosa; Intestines; Lipid Metabolism; Liver; Male; Micelles; Rats; Rats, Sprague-Dawley; Solid Phase Extraction

2008
Probing conformational changes of prestin with thiol-reactive optical switches.
    Biophysical journal, 2008, Sep-15, Volume: 95, Issue:6

    Topics: Animals; Anion Transport Proteins; Biotin; Cell Membrane; Cysteine; Electric Capacitance; Guinea Pigs; Hair Cells, Auditory, Outer; Humans; Iodoacetamide; Maleimides; Optics and Photonics; Protein Conformation; Sulfhydryl Compounds; Transfection

2008
Targeting of adenovirus vectors to the LRP receptor family with the high-affinity ligand RAP via combined genetic and chemical modification of the pIX capsomere.
    Molecular therapy : the journal of the American Society of Gene Therapy, 2008, Volume: 16, Issue:11

    Topics: Adenoviridae; Animals; Biotin; Capsid Proteins; Cell Line; Cysteine; Female; Genetic Vectors; Humans; LDL-Receptor Related Protein-Associated Protein; LDL-Receptor Related Proteins; Ligands; Maleimides; Mice; Mice, Inbred BALB C; Receptors, Transferrin; Transferrin; Virion

2008
Alteration of S-nitrosothiol homeostasis and targets for protein S-nitrosation in human hepatocytes.
    Proteomics, 2008, Volume: 8, Issue:22

    Topics: Adult; Aged; Aldehyde Oxidoreductases; Analysis of Variance; Biotin; Chromatography, Liquid; Cysteine; Female; Hepatocytes; Homeostasis; Humans; Liver Diseases; Male; Middle Aged; Nitric Oxide; Nitrosation; Phosphatidylethanolamine Binding Protein; Protein Disulfide-Isomerases; Proteins; S-Nitrosothiols; Tandem Mass Spectrometry; Tumor Cells, Cultured

2008
Two highly conserved cysteine residues in HPV16 L2 form an intramolecular disulfide bond and are critical for infectivity in human keratinocytes.
    PloS one, 2009, Volume: 4, Issue:2

    Topics: Amino Acid Sequence; Animals; Biotin; Capsid Proteins; Cell Line; Cysteine; Disulfides; Human papillomavirus 16; Humans; Keratinocytes; Molecular Sequence Data; Oncogene Proteins, Viral; Oxidation-Reduction; Sequence Alignment; Virion

2009
Analysis of oxidative modification of proteins.
    Current protocols in protein science, 2009, Volume: Chapter 14

    Topics: Biochemistry; Biotin; Borohydrides; Cysteine; Isoaspartic Acid; Isotope Labeling; Oxidation-Reduction; Phenylhydrazines; Protein Binding; Proteins; Sulfhydryl Compounds; Tyrosine

2009
Synthesis and application of a N-1' fluorescent biotinyl derivative inducing the specific carboxy-terminal dual labeling of a novel RhoB-selective scFv.
    Bioconjugate chemistry, 2009, May-20, Volume: 20, Issue:5

    Topics: Antibody Specificity; Binding Sites; Biotin; Cysteine; Esters; Fluorescent Antibody Technique; Fluorescent Dyes; HeLa Cells; Humans; Inteins; Nitrogen; Protein Splicing; rhoB GTP-Binding Protein; Single-Chain Antibodies; Staining and Labeling; Substrate Specificity

2009
Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture.
    Nature biotechnology, 2009, Volume: 27, Issue:6

    Topics: Biotin; Cell Line; Cysteine; Glyceraldehyde-3-Phosphate Dehydrogenases; Humans; Mass Spectrometry; Proteome; Proteomics; S-Nitrosothiols; Sensitivity and Specificity; Sulfhydryl Compounds

2009
Use of intein-mediated protein ligation strategies for the fabrication of functional protein arrays.
    Methods in enzymology, 2009, Volume: 462

    Topics: Animals; Avidin; Biotin; Biotinylation; Cysteine; Gene Expression; Green Fluorescent Proteins; Humans; Immobilized Proteins; Inteins; Peptides; Protein Array Analysis; Protein Splicing; Protein Structure, Tertiary; Proteins; Puromycin; Recombinant Fusion Proteins; Sulfhydryl Reagents

2009
Novel twist on the biotin switch cracks endothelial cell S-nitrosoproteome.
    Journal of proteome research, 2009, Volume: 8, Issue:10

    Topics: Biotin; Cysteine; Penicillamine; Proteins; Proteomics; S-Nitroso-N-Acetylpenicillamine; S-Nitrosothiols

2009
S-Nitrosylation of DRP1 does not affect enzymatic activity and is not specific to Alzheimer's disease.
    Journal of Alzheimer's disease : JAD, 2010, Volume: 20 Suppl 2

    Topics: Aging; Alzheimer Disease; Biotin; Brain; Cell Line, Transformed; Cysteine; Dithiothreitol; Dynamins; GTP Phosphohydrolases; Humans; Microscopy, Electron, Transmission; Microtubule-Associated Proteins; Mitochondrial Diseases; Mitochondrial Proteins; Postmortem Changes; Protein Multimerization

2010
Epitope mapping in cell surface proteins by site-directed masking: defining the structural elements of NTPDase3 inhibition by a monoclonal antibody.
    Protein engineering, design & selection : PEDS, 2010, Volume: 23, Issue:7

    Topics: Adenosine Triphosphatases; Animals; Antibodies, Monoclonal; Binding Sites, Antibody; Biotin; Chlorocebus aethiops; COS Cells; Cysteine; Enzymes, Immobilized; Epitope Mapping; Epitopes; Humans; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Rats

2010
Proteomic and mass spectroscopic quantitation of protein S-nitrosation differentiates NO-donors.
    ACS chemical biology, 2010, Jul-16, Volume: 5, Issue:7

    Topics: Biotin; Cells, Cultured; Cysteine; Glutathione S-Transferase pi; Humans; Mass Spectrometry; Neuroblastoma; Nitric Oxide; Nitric Oxide Donors; Nitrosation; Proteins; Proteomics; S-Nitrosoglutathione; S-Nitrosothiols

2010
Selective covalent labeling of tag-fused GPCR proteins on live cell surface with a synthetic probe for their functional analysis.
    Journal of the American Chemical Society, 2010, Jul-14, Volume: 132, Issue:27

    Topics: Biotin; Cell Line; Cysteine; Endocytosis; Endosomes; Fluoresceins; Humans; Molecular Probe Techniques; Molecular Probes; Oligopeptides; Organometallic Compounds; Receptors, G-Protein-Coupled; Staining and Labeling

2010
Acyl-biotinyl exchange chemistry and mass spectrometry-based analysis of palmitoylation sites of in vitro palmitoylated rat brain tubulin.
    The protein journal, 2010, Volume: 29, Issue:8

    Topics: Amino Acid Sequence; Animals; Bacterial Proteins; Binding Sites; Biotin; Brain; Cysteine; Lipoylation; Mass Spectrometry; Molecular Sequence Data; Palmitic Acid; Protein Engineering; Proteomics; Rats; Sequence Analysis; Signal Transduction; Tubulin

2010
S-nitrosylation of cyclin-dependent kinase 5 (cdk5) regulates its kinase activity and dendrite growth during neuronal development.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 2010, Oct-27, Volume: 30, Issue:43

    Topics: Animals; Biotin; Brain Chemistry; Cells, Cultured; Cyclin-Dependent Kinase 5; Cysteine; Dendrites; DNA, Complementary; Hippocampus; Humans; Mice; Neurons; Nitroso Compounds; Protein Processing, Post-Translational; Recombinant Fusion Proteins; Transfection

2010
Nitrosative protein oxidation is modulated during early endotoxemia.
    Nitric oxide : biology and chemistry, 2011, Aug-01, Volume: 25, Issue:2

    Topics: Animals; Biotin; Blood Pressure; Cysteine; Endotoxemia; Escherichia coli; Heart; HEK293 Cells; Humans; Hypotension; Immunoblotting; In Vitro Techniques; Lipopolysaccharides; Mass Spectrometry; Metalloporphyrins; Mice; Mice, Inbred C57BL; Models, Animal; Nitric Oxide; Nitric Oxide Synthase; Oxidation-Reduction; Peroxynitrous Acid; Protein Processing, Post-Translational; Proteins; Rats; Rats, Wistar; S-Nitroso-N-Acetylpenicillamine; Sensitivity and Specificity; Sepsis; Signal Transduction; Telemetry; Tyrosine

2011
In vitro selection of proteins that undergo covalent labeling with small molecules by thiol-disulfide exchange by using ribosome display.
    Chembiochem : a European journal of chemical biology, 2011, Apr-11, Volume: 12, Issue:6

    Topics: Amino Acid Sequence; Amino Acid Substitution; Biotin; Cysteine; Disulfides; Methionine; Molecular Sequence Data; Mutant Proteins; Mutation; Peptides; Protein Engineering; Protein Structure, Tertiary; Ribosomes; Sulfhydryl Compounds; Sulfur Radioisotopes

2011
Purification of tetracysteine-tagged proteins by affinity chromatography using a non-fluorescent, photochemically stable bisarsenical affinity ligand.
    Bioconjugate chemistry, 2011, May-18, Volume: 22, Issue:5

    Topics: Arsenicals; Biotin; Chromatography, Affinity; Cysteine; Kinetics; Ligands; Molecular Structure; Photochemical Processes; Proteins; Stereoisomerism

2011
Versatile bio-ink for covalent immobilization of chimeric avidin on sol-gel substrates.
    Colloids and surfaces. B, Biointerfaces, 2011, Oct-15, Volume: 87, Issue:2

    Topics: Avidin; Biocompatible Materials; Biotin; Chromatography, Affinity; Cloning, Molecular; Cross-Linking Reagents; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Gels; Immobilized Proteins; Ink; Phase Transition; Plasmids; Polymerase Chain Reaction; Polymers; Propylamines; Protein Binding; Protein Engineering; Recombinant Proteins; Silanes; Succinimides; Surface Properties

2011
Agonist- and hydrogen peroxide-mediated oxidation of the β2 adrenergic receptor: evidence of receptor s-sulfenation as detected by a modified biotin-switch assay.
    The Journal of pharmacology and experimental therapeutics, 2011, Volume: 339, Issue:3

    Topics: Adrenergic beta-2 Receptor Agonists; Alkylation; Biological Assay; Biotin; Cyclohexanones; Cysteine; Dose-Response Relationship, Drug; Epithelial Cells; Ethanolamines; Formoterol Fumarate; HEK293 Cells; Humans; Hydrogen Peroxide; Isoproterenol; Oxidation-Reduction; Pulmonary Alveoli; Reactive Oxygen Species; Receptors, Adrenergic, beta-2; Signal Transduction; Sulfenic Acids; Time Factors

2011
An affinity purification procedure to isolate oxidized p53.
    Analytical biochemistry, 2012, Jan-01, Volume: 420, Issue:1

    Topics: Avidin; Biotin; Chromatography, Affinity; Cysteine; Humans; Oxidation-Reduction; Sulfhydryl Compounds; Tumor Suppressor Protein p53

2012
N-biotinyl-S-(1,2-dichlorovinyl)-L-cysteine sulfoxide as a potential model for S-(1,2-dichlorovinyl)-L-cysteine sulfoxide: characterization of stability and reactivity with glutathione and kidney proteins in vitro.
    Chemical research in toxicology, 2011, Nov-21, Volume: 24, Issue:11

    Topics: Animals; Binding Sites; Binding, Competitive; Biotin; Biotinylation; Blotting, Western; Cysteine; Dose-Response Relationship, Drug; Drug Stability; Electrophoresis, Polyacrylamide Gel; Glutathione Reductase; Half-Life; Humans; Indicators and Reagents; Kidney; Kidney Neoplasms; Malate Dehydrogenase; Protein Binding; Rats, Sprague-Dawley

2011
Cys(294) is essential for the function of the human sodium-dependent multivitamin transporter.
    Biochimica et biophysica acta, 2012, Volume: 1818, Issue:1

    Topics: Biological Transport; Biotin; Biotinylation; Cell Membrane; Cysteine; DNA Primers; Epithelial Cells; Humans; Kinetics; Mutagenesis, Site-Directed; Mutation; Polymerase Chain Reaction; Retina; Structure-Activity Relationship; Symporters; Transfection

2012
DNA family shuffling within the chicken avidin protein family - A shortcut to more powerful protein tools.
    Journal of biotechnology, 2012, Volume: 157, Issue:1

    Topics: Amino Acid Sequence; Animals; Avidin; Biotin; Calorimetry, Differential Scanning; Chickens; Cysteine; Directed Molecular Evolution; DNA Shuffling; Molecular Sequence Data; Mutation; Protein Stability; Recombinant Fusion Proteins; Sequence Alignment

2012
Identification and quantification of S-nitrosylation by cysteine reactive tandem mass tag switch assay.
    Molecular & cellular proteomics : MCP, 2012, Volume: 11, Issue:2

    Topics: Biotin; Blotting, Western; Cells, Cultured; Cysteine; Endothelium, Vascular; Humans; Nitric Oxide; Oxidation-Reduction; Protein Processing, Post-Translational; Proteomics; Pulmonary Artery; S-Nitrosoglutathione; Sulfhydryl Compounds; Tandem Mass Spectrometry

2012
Site-specific immobilization of enzymes on magnetic nanoparticles and their use in organic synthesis.
    Bioconjugate chemistry, 2012, Apr-18, Volume: 23, Issue:4

    Topics: Binding Sites; Biotin; Chemistry Techniques, Synthetic; Cysteine; Enzymes, Immobilized; Kinetics; Magnetite Nanoparticles; Neisseria gonorrhoeae; Oxo-Acid-Lyases; Pasteurella multocida; Polyethylene Glycols; Sialyltransferases; Streptavidin; Substrate Specificity

2012
Purification of histidine-tagged aequorin with a reactive cysteine residue for chemical conjugations and its application for bioluminescent sandwich immunoassays.
    Protein expression and purification, 2012, Volume: 83, Issue:2

    Topics: Aequorin; alpha-Fetoproteins; Biotin; Cysteine; Histidine; Immunoassay; Luminescent Measurements; Maleimides; Models, Chemical; Recombinant Fusion Proteins; Sensitivity and Specificity; Streptavidin

2012
Improving the selectivity of the phosphoric acid β-elimination on a biotinylated phosphopeptide.
    Journal of the American Society for Mass Spectrometry, 2012, Volume: 23, Issue:11

    Topics: Acetonitriles; Biotin; Cysteine; Hydrogen Sulfide; Limit of Detection; Phosphopeptides; Phosphoric Acids; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Temperature; Water

2012
S-nitrosylated protein disulfide isomerase contributes to mutant SOD1 aggregates in amyotrophic lateral sclerosis.
    Journal of neurochemistry, 2013, Volume: 124, Issue:1

    Topics: Amyotrophic Lateral Sclerosis; Animals; Arginine; Biotin; Cell Line, Tumor; Cysteine; Disease Models, Animal; Disease Progression; Humans; Male; Mice; Mice, Transgenic; Mutation; Neuroblastoma; Nitric Oxide; Nitric Oxide Synthase; Nitric Oxide Synthase Type II; Protein Disulfide-Isomerases; Protein Folding; S-Nitrosothiols; Spinal Cord; Subcellular Fractions; Superoxide Dismutase; Up-Regulation

2013
Chasing cysteine oxidative modifications: proteomic tools for characterizing cysteine redox status.
    Circulation. Cardiovascular genetics, 2012, Oct-01, Volume: 5, Issue:5

    Topics: Acylation; Biotin; Chromatography, High Pressure Liquid; Cysteine; Glutathione; Humans; Oxidation-Reduction; Proteomics; Reactive Oxygen Species; Sulfhydryl Compounds; Sulfinic Acids; Sulfonic Acids; Tandem Mass Spectrometry

2012
Long range effect of mutations on specific conformational changes in the extracellular loop 2 of angiotensin II type 1 receptor.
    The Journal of biological chemistry, 2013, Jan-04, Volume: 288, Issue:1

    Topics: Animals; Biotin; Calcium; Chlorocebus aethiops; COS Cells; Cysteine; Ligands; Molecular Conformation; Molecular Dynamics Simulation; Mutagenesis; Mutation; Protein Conformation; Protein Structure, Tertiary; Rats; Receptor, Angiotensin, Type 1; Receptors, G-Protein-Coupled; Signal Transduction

2013
Rocket fuel for the quantification of S-nitrosothiols. Highly specific reduction of S-nitrosothiols to thiols by methylhydrazine.
    Free radical research, 2013, Volume: 47, Issue:2

    Topics: Ascorbic Acid; Biotin; Cysteine; Glutathione; Magnetic Resonance Spectroscopy; Methylhydrazines; Nitric Oxide; Nitrosation; Oxidation-Reduction; Proteins; Reactive Nitrogen Species; Reactive Oxygen Species; S-Nitrosothiols; Sulfhydryl Compounds

2013
Triple cysteine module within M-type K+ channels mediates reciprocal channel modulation by nitric oxide and reactive oxygen species.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 2013, Apr-03, Volume: 33, Issue:14

    Topics: Action Potentials; Aniline Compounds; Animals; Animals, Newborn; Anthracenes; Benzoates; Biotin; Calcitonin Gene-Related Peptide; Carbamates; Cells, Cultured; Cysteine; Enzyme Inhibitors; Female; Humans; Imidazoles; KCNQ Potassium Channels; Male; Membrane Transport Modulators; Models, Molecular; Mutation; Neurons; Nitric Oxide; Nitric Oxide Donors; Patch-Clamp Techniques; Phenylenediamines; Potassium Channel Blockers; Rats; Reactive Oxygen Species; S-Nitroso-N-Acetylpenicillamine; Transfection; Trigeminal Ganglion

2013
Quantification of mitochondrial S-nitrosylation by CysTMT⁶ switch assay.
    Methods in molecular biology (Clifton, N.J.), 2013, Volume: 1005

    Topics: Animals; Biological Assay; Biotin; Cysteine; Mitochondria, Heart; Mitochondrial Proteins; Myocardium; Nitric Oxide; Oxidation-Reduction; Protein Processing, Post-Translational; Rats; S-Nitrosoglutathione

2013
Effect of length of molecular recognition moiety on enzymatic activity switching.
    Journal of bioscience and bioengineering, 2013, Volume: 116, Issue:4

    Topics: Avidin; Biotin; Camphor 5-Monooxygenase; Catalytic Domain; Cysteine; Mutagenesis, Site-Directed; Polyethylene Glycols

2013
Convenient method to assess chemical modification of protein thiols by electrophilic metals.
    The Journal of toxicological sciences, 2013, Volume: 38, Issue:3

    Topics: Adaptor Proteins, Signal Transducing; Animals; Biotin; Cysteine; Cytoskeletal Proteins; Electrophoresis, Polyacrylamide Gel; Hepatocytes; Humans; Kelch-Like ECH-Associated Protein 1; Male; Maleimides; Methylmercury Compounds; Mice; Mice, Inbred C57BL; Neuroblastoma; Sulfhydryl Compounds; Tumor Cells, Cultured

2013
Solid-phase synthesis of Biotin-S-Farnesyl-L-Cysteine, a surrogate substrate for isoprenylcysteine Carboxylmethyltransferase (ICMT).
    Bioorganic & medicinal chemistry letters, 2013, Oct-15, Volume: 23, Issue:20

    Topics: Biotin; Cysteine; High-Throughput Screening Assays; Kinetics; Protein Methyltransferases; Solid-Phase Synthesis Techniques; Substrate Specificity; Trityl Compounds

2013
Amplified electrochemiluminescence of luminol based on hybridization chain reaction and in situ generate co-reactant for highly sensitive immunoassay.
    Talanta, 2013, Oct-15, Volume: 115

    Topics: Animals; Antibodies, Anti-Idiotypic; Biotin; Biotinylation; Cysteine; DNA, Single-Stranded; Electrodes; Enzymes, Immobilized; Glucose; Glucose Oxidase; Goats; Graphite; Humans; Hydrogen Peroxide; Immunoassay; Immunoglobulin G; Limit of Detection; Luminescent Measurements; Luminol; Mice; Oxides; Streptavidin

2013
Selective conjugation of proteins by mining active proteomes through click-functionalized magnetic nanoparticles.
    ACS nano, 2013, Nov-26, Volume: 7, Issue:11

    Topics: Azides; Biotin; Click Chemistry; Cysteine; Data Mining; Ferric Compounds; Leucine; Luminescence; Magnetics; Microwaves; Nanoparticles; Nanotechnology; Peptide Hydrolases; Plant Proteins; Proteome; Rhodamines; Spectroscopy, Fourier Transform Infrared; Streptavidin; Thermogravimetry

2013
Differential S-nitrosylation of proteins in Alzheimer's disease.
    Neuroscience, 2014, Jan-03, Volume: 256

    Topics: Aged; Alzheimer Disease; Biotin; Brain; Case-Control Studies; Cysteine; Electrophoresis, Gel, Two-Dimensional; Female; Fructose-Bisphosphate Aldolase; Humans; Male; Proteomics; S-Nitrosothiols; Superoxide Dismutase; Tandem Mass Spectrometry; Voltage-Dependent Anion Channel 2

2014
Detection of protein S-sulfhydration by a tag-switch technique.
    Angewandte Chemie (International ed. in English), 2014, Jan-07, Volume: 53, Issue:2

    Topics: Biotin; Cysteine; Glutathione Peroxidase; Human Umbilical Vein Endothelial Cells; Humans; Hydrogen Sulfide; Jurkat Cells; Microscopy, Fluorescence; Protein Processing, Post-Translational; Serum Albumin, Bovine; Sulfhydryl Compounds; Sulfides

2014
S-nitrosylation influences the structure and DNA binding activity of AtMYB30 transcription factor from Arabidopsis thaliana.
    Biochimica et biophysica acta, 2014, Volume: 1844, Issue:4

    Topics: Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Binding Sites; Biological Assay; Biotin; Circular Dichroism; Cysteine; DNA, Plant; Escherichia coli; Molecular Sequence Data; Mutagenesis, Site-Directed; Nitric Oxide; Nitric Oxide Donors; Protein Binding; Protein Structure, Secondary; Recombinant Proteins; Sequence Alignment; Transcription Factors

2014
Oxygen-linked S-nitrosation in fish myoglobins: a cysteine-specific tertiary allosteric effect.
    PloS one, 2014, Volume: 9, Issue:5

    Topics: Allosteric Regulation; Amino Acid Sequence; Animals; Biotin; Cysteine; Fish Proteins; Fishes; Heme; Humans; Kinetics; Molecular Sequence Data; Myocardium; Myoglobin; Nitric Oxide; Nitrosation; Oxygen; Species Specificity

2014
Sulfhydryl-specific probe for monitoring protein redox sensitivity.
    ACS chemical biology, 2014, Dec-19, Volume: 9, Issue:12

    Topics: Amino Acid Sequence; Biotin; Biotinylation; Cysteine; HeLa Cells; Humans; Hydrogen Peroxide; Molecular Probes; Molecular Sequence Data; Nitrobenzoates; Oxidation-Reduction; Oxidative Stress; Proteins; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining and Labeling; Streptavidin; Sulfhydryl Compounds; Sulfonamides

2014
Endogenous S-sulfhydration of PTEN helps protect against modification by nitric oxide.
    Biochemical and biophysical research communications, 2015, Jan-02, Volume: 456, Issue:1

    Topics: Biotin; Cell Line, Tumor; Cystathionine beta-Synthase; Cysteine; Humans; Hydrogen Sulfide; Mutagenesis, Site-Directed; Neuroblastoma; Neurons; Nitric Oxide; Oxidative Stress; Plasmids; Proto-Oncogene Proteins c-akt; PTEN Phosphohydrolase; RNA, Small Interfering; Signal Transduction; Sulfur; Transfection

2015
Use of the "tag-switch" method for the detection of protein S-sulfhydration.
    Methods in enzymology, 2015, Volume: 555

    Topics: Acetates; Benzothiazoles; Biotin; Cysteine; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); Human Umbilical Vein Endothelial Cells; Humans; Hydrogen Sulfide; Jurkat Cells; Microscopy, Fluorescence; Oxidation-Reduction; Protein Processing, Post-Translational; Serum Albumin, Bovine; Signal Transduction; Spectrometry, Mass, Electrospray Ionization; Staining and Labeling; Streptavidin; Sulfides

2015
Protein sulfhydration.
    Methods in enzymology, 2015, Volume: 555

    Topics: Biotin; Cell Line; Cysteine; Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); Humans; Hydrogen Sulfide; Maleimides; Morpholines; Organothiophosphorus Compounds; Oxidation-Reduction; Phenanthrolines; Protein Processing, Post-Translational; Signal Transduction; Staining and Labeling; Streptavidin; Sulfides

2015
Identification of total reversible cysteine oxidation in an atherosclerosis model using a modified biotin switch assay.
    Journal of proteome research, 2015, May-01, Volume: 14, Issue:5

    Topics: Amino Acid Sequence; Atherosclerosis; Biological Assay; Biotin; Blood Platelets; Cell Extracts; Cell Line; Chromatography, Liquid; Cysteine; Humans; Metabolic Networks and Pathways; Models, Biological; Molecular Sequence Annotation; Molecular Sequence Data; Monocytes; NADPH Oxidases; Oxidation-Reduction; Peptides; Primary Cell Culture; Proteome; Reactive Oxygen Species; Tandem Mass Spectrometry

2015
S-sulfhydration: a cysteine posttranslational modification in plant systems.
    Plant physiology, 2015, Volume: 168, Issue:1

    Topics: Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Ascorbate Peroxidases; Biotin; Cysteine; Cytosol; Immunoblotting; Mass Spectrometry; Molecular Sequence Data; Protein Processing, Post-Translational; Recombinant Proteins; Sulfides

2015
Synthetic methodology for asymmetric ferrocene derived bio-conjugate systems via solid phase resin-based methodology.
    Journal of visualized experiments : JoVE, 2015, Mar-12, Issue:97

    Topics: Biotin; Cysteine; Ferrous Compounds; Gold; Metallocenes; Plastics; Solid-Phase Synthesis Techniques; Surface Properties

2015
ATP Binding and Hydrolysis Properties of ABCB10 and Their Regulation by Glutathione.
    PloS one, 2015, Volume: 10, Issue:6

    Topics: Adenosine Triphosphate; Aminolevulinic Acid; Animals; ATP-Binding Cassette Transporters; Azides; Biotin; Conserved Sequence; Cysteine; Glutathione; Glutathione Disulfide; HEK293 Cells; Humans; Hydrolysis; Mice; Mitochondria; Mutation; Protein Structure, Tertiary; Submitochondrial Particles

2015
Dual Labeling Biotin Switch Assay to Reduce Bias Derived From Different Cysteine Subpopulations: A Method to Maximize S-Nitrosylation Detection.
    Circulation research, 2015, Oct-23, Volume: 117, Issue:10

    Topics: Alcohol Dehydrogenase; Animals; Biotin; Cysteine; Female; HEK293 Cells; Humans; Male; Mice, Inbred C57BL; Mice, Knockout; Molecular Probes; Myocardium; Nitrosation; Nitroso Compounds; Protein Processing, Post-Translational; Proteomics; Reproducibility of Results; Tandem Mass Spectrometry

2015
Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling.
    PloS one, 2015, Volume: 10, Issue:9

    Topics: Amino Acid Sequence; Biotin; Biotinylation; Carrier Proteins; Chromatography, Affinity; Cysteine; Electrophoresis, Polyacrylamide Gel; Kinetics; Ligands; Maltose-Binding Proteins; Models, Molecular; Molecular Sequence Data; Peptides; Protein Binding; Protein Engineering; Recombinant Fusion Proteins; Streptavidin; Sulfhydryl Compounds

2015
The Cysteine-rich Domain of the DHHC3 Palmitoyltransferase Is Palmitoylated and Contains Tightly Bound Zinc.
    The Journal of biological chemistry, 2015, Dec-04, Volume: 290, Issue:49

    Topics: Amino Acid Motifs; Animals; Biotin; Catalytic Domain; Chelating Agents; Cysteine; Iodoacetamide; Ions; Lipoylation; Lysine; Mass Spectrometry; Membrane Proteins; Metals; Mice; Mutation; Protein Binding; Protein Processing, Post-Translational; Sf9 Cells; Spodoptera; Zinc

2015
SNOs Differ: Methodological and Biological Implications.
    Circulation research, 2015, Oct-23, Volume: 117, Issue:10

    Topics: Animals; Biotin; Cysteine; Female; Humans; Male; Molecular Probes; Myocardium; Nitroso Compounds; Proteomics

2015
The "π-Clamp" Offers a New Strategy for Site-Selective Protein Modification.
    Chembiochem : a European journal of chemical biology, 2016, 05-17, Volume: 17, Issue:10

    Topics: Biotin; Catalysis; Cysteine; Fluorocarbons; Glutathione Transferase; Protein Processing, Post-Translational; Proteins

2016
Cloning, expression and characterization of histidine-tagged biotin synthase of Mycobacterium tuberculosis.
    Tuberculosis (Edinburgh, Scotland), 2016, Volume: 98

    Topics: Antitubercular Agents; Bacterial Proteins; Biotin; Cloning, Molecular; Cysteine; Deoxyadenosines; Enzyme Inhibitors; Enzyme Stability; Histidine; Hydrogen-Ion Concentration; Kinetics; Microbial Sensitivity Tests; Mycobacterium tuberculosis; Protein Engineering; Protein Multimerization; Recombinant Fusion Proteins; S-Adenosylhomocysteine; S-Adenosylmethionine; Substrate Specificity; Sulfurtransferases; Temperature

2016
[Reactive sulfur species-modified protein thiols: new methods for polysulfurated protein analysis].
    Nihon yakurigaku zasshi. Folia pharmacologica Japonica, 2016, Volume: 147, Issue:5

    Topics: Biotin; Cyanates; Cysteine; Disulfides; Humans; Proteins; Proteomics; Reagent Kits, Diagnostic; Sulfhydryl Compounds; Sulfides; Sulfur; Tumor Cells, Cultured

2016
Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells.
    Methods in molecular biology (Clifton, N.J.), 2016, Volume: 1437

    Topics: Biotin; Blotting, Western; Cells, Cultured; Connexin 43; Cysteine; Fluorescent Antibody Technique; Human Umbilical Vein Endothelial Cells; Humans; Phosphorylation; Primary Cell Culture; Protein Processing, Post-Translational; S-Nitrosothiols; Serine; Threonine; Tyrosine

2016
Biotin Switch Assays for Quantitation of Reversible Cysteine Oxidation.
    Methods in enzymology, 2017, Volume: 585

    Topics: Biological Assay; Biotin; Cysteine; Oxidation-Reduction

2017
Development of a Small Hybrid Molecule That Mediates Degradation of His-Tag Fused Proteins.
    Journal of medicinal chemistry, 2018, 01-25, Volume: 61, Issue:2

    Topics: Biotin; Cell Line; Cysteine; Histidine; Humans; Leupeptins; Maleimides; Nitrilotriacetic Acid; Organometallic Compounds; Proteasome Inhibitors; Proteolysis; Receptors, Retinoic Acid; Recombinant Fusion Proteins; Smad2 Protein; Ubiquitin-Protein Ligases

2018
Chemical addressability of potato virus X for its applications in bio/nanotechnology.
    Journal of structural biology, 2017, Volume: 200, Issue:3

    Topics: Biotechnology; Biotin; Biotinylation; Contrast Media; Cysteine; Electrophoresis, Polyacrylamide Gel; Fluorescent Dyes; Heterocyclic Compounds; Immunohistochemistry; Magnetic Resonance Imaging; Microscopy, Electron, Transmission; Nanoparticles; Nanotechnology; Organometallic Compounds; Potexvirus; Tandem Mass Spectrometry

2017
Release of Enzymatically Active Deubiquitinating Enzymes upon Reversible Capture by Disulfide Ubiquitin Reagents.
    Angewandte Chemie (International ed. in English), 2017, 10-09, Volume: 56, Issue:42

    Topics: Biotin; Catalytic Domain; Cysteine; Deubiquitinating Enzymes; Disulfides; HeLa Cells; Humans; Polyethylene Glycols; Proteins; Ubiquitin; Ubiquitination

2017
Native-MS Analysis of Monoclonal Antibody Conjugates by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
    Analytical chemistry, 2018, 01-02, Volume: 90, Issue:1

    Topics: Antibodies, Monoclonal; Biotin; Cysteine; Immunoconjugates; Immunoglobulin G; Lysine; Mass Spectrometry

2018
Monodisperse measurement of the biotin-streptavidin interaction strength in a well-defined pulling geometry.
    PloS one, 2017, Volume: 12, Issue:12

    Topics: Biotin; Calorimetry; Cysteine; Electrophoresis, Polyacrylamide Gel; Microscopy, Atomic Force; Streptavidin

2017
Artificial Cysteine S-Glycosylation Induced by Per-O-Acetylated Unnatural Monosaccharides during Metabolic Glycan Labeling.
    Angewandte Chemie (International ed. in English), 2018, 02-12, Volume: 57, Issue:7

    Topics: Acetylation; Alkynes; Azides; Biotin; Cysteine; Glutathione; Glycosylation; HeLa Cells; Humans; Metabolic Engineering; Monosaccharides; Peptides; Tandem Mass Spectrometry

2018
Identification of S-Nitrosylated and Reversibly Oxidized Proteins by Fluorescence Switch and Complementary Techniques.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1747

    Topics: Biotin; Cysteine; Fluorescence; Mass Spectrometry; Nitric Oxide; Oxidation-Reduction; Protein Processing, Post-Translational; Proteomics; Staining and Labeling

2018
Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Technique.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1747

    Topics: Biotin; Cysteine; Nitric Oxide; Nitrosation; Oxidation-Reduction; Protein Conformation; Protein Processing, Post-Translational; Recombinant Proteins

2018
Identification of NO-Sensitive Cysteine Residues Using Cysteine Mutants of Recombinant Proteins.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1747

    Topics: Arabidopsis; Arabidopsis Proteins; Biotin; Chromatography, Affinity; Cloning, Molecular; Cysteine; Gene Expression; Mutagenesis, Site-Directed; Mutation; Nitric Oxide; Nitrosation; Recombinant Proteins

2018
Site-Specific Protein Labeling with N-Hydroxysuccinimide-Esters and the Analysis of Ubiquitin Ligase Mechanisms.
    Journal of the American Chemical Society, 2018, 08-01, Volume: 140, Issue:30

    Topics: Animals; Biotin; Cysteine; Escherichia coli; Esters; Fluoresceins; Fluorescent Dyes; Glutathione Transferase; Humans; Protein Binding; PTEN Phosphohydrolase; Rhodamines; Saccharomyces cerevisiae; Spodoptera; Succinimides; Ubiquitin; Ubiquitin-Protein Ligases; Ubiquitination; Uracil-DNA Glycosidase

2018
Identification of S-nitrosylated proteins in postmortem pork muscle using modified biotin switch method coupled with isobaric tags.
    Meat science, 2018, Volume: 145

    Topics: Animals; Biotin; Chromatography, Liquid; Cysteine; Dietary Proteins; Food Analysis; Humans; Muscle Proteins; Muscles; Nitric Oxide; Nitroso Compounds; Peptides; Postmortem Changes; Proteolysis; Red Meat; S-Nitrosoglutathione; Sulfhydryl Compounds; Swine; Tandem Mass Spectrometry

2018
Building and Breaking Bonds via a Compact S-Propargyl-Cysteine to Chemically Control Enzymes and Modify Proteins.
    Angewandte Chemie (International ed. in English), 2018, 09-24, Volume: 57, Issue:39

    Topics: 3C Viral Proteases; Archaeal Proteins; Biotin; Catalysis; Catalytic Domain; Click Chemistry; Cysteine; Cysteine Endopeptidases; Enterovirus; Green Fluorescent Proteins; Humans; Methanosarcina; Mutagenesis, Site-Directed; Palladium; Pargyline; Thioredoxins; Viral Proteins

2018
High-Affinity Antibody Detection with a Bivalent Circularized Peptide Containing Antibody-Binding Domains.
    Biotechnology journal, 2019, Volume: 14, Issue:5

    Topics: Amino Acid Sequence; Antibody Affinity; Bacterial Proteins; Binding Sites; Binding Sites, Antibody; Biotin; Chromatography, Affinity; Chromatography, Ion Exchange; Cysteine; Enzyme-Linked Immunosorbent Assay; Fungal Proteins; Humans; Immobilization; Immobilized Proteins; Immunoglobulin Fab Fragments; Immunoglobulin G; Kinetics; Models, Molecular; Molecular Probe Techniques; Peptide Hydrolases; Peptides; Protein Binding; Protein Domains; Substrate Specificity; Yeasts

2019
Real-time microscopy and physical perturbation of bacterial pili using maleimide-conjugated molecules.
    Nature protocols, 2019, Volume: 14, Issue:6

    Topics: Biotin; Caulobacter; Cysteine; Fimbriae, Bacterial; Fluorescent Dyes; Maleimides; Microscopy, Fluorescence; Models, Molecular; Optical Imaging; Staining and Labeling; Vibrio cholerae

2019
Determining the Redox Potential of a Protein Disulphide Bond.
    Methods in molecular biology (Clifton, N.J.), 2019, Volume: 1967

    Topics: Biotin; Cysteine; Disulfides; Maleimides; Oxidation-Reduction; Protein Domains; Protein Folding; Proteins; Sulfhydryl Compounds; Tandem Mass Spectrometry

2019
Evaluation of Chemically-Cleavable Linkers for Quantitative Mapping of Small Molecule-Cysteinome Reactivity.
    ACS chemical biology, 2019, 09-20, Volume: 14, Issue:9

    Topics: Azo Compounds; Biotin; Cell Line, Tumor; Cysteine; Humans; Protein Binding; Proteome; Proteomics; Quinazolinones; Silanes

2019
Site-Specific Quantification of Persulfidome by Combining an Isotope-Coded Affinity Tag with Strong Cation-Exchange-Based Fractionation.
    Analytical chemistry, 2019, 12-03, Volume: 91, Issue:23

    Topics: A549 Cells; Biotin; Carbon Isotopes; Chromatography, Ion Exchange; Cysteine; Epithelial-Mesenchymal Transition; HeLa Cells; Humans; Isotope Labeling; Protein Processing, Post-Translational; Proteome; Reference Standards; Serum Albumin, Bovine; Streptavidin; Sulfides; Tandem Mass Spectrometry

2019
Isotopically Labeled Desthiobiotin Azide (isoDTB) Tags Enable Global Profiling of the Bacterial Cysteinome.
    Angewandte Chemie (International ed. in English), 2020, 02-10, Volume: 59, Issue:7

    Topics: Azides; Biotin; Cysteine; Isotope Labeling; Molecular Structure; Staphylococcus aureus

2020
Discovery of a Redox Thiol Switch: Implications for Cellular Energy Metabolism.
    Molecular & cellular proteomics : MCP, 2020, Volume: 19, Issue:5

    Topics: Activating Transcription Factor 4; Animals; Biological Assay; Biotin; Cell Line; Cysteine; Disulfides; Energy Metabolism; Glycolysis; Hepatocytes; Humans; Hydrogen Sulfide; Insulin-Secreting Cells; Mass Spectrometry; Metabolic Flux Analysis; Mitochondria; Oxidation-Reduction; Proteome; Proteomics; Rats; Sulfhydryl Compounds; Sulfides

2020
Site-Directed Modification of Yeast-Produced Proteins Using Expressed Protein Ligation.
    Methods in molecular biology (Clifton, N.J.), 2020, Volume: 2133

    Topics: Azides; Biotin; Blotting, Western; Cell Surface Display Techniques; Chemistry Techniques, Synthetic; Click Chemistry; Cysteine; Fungal Proteins; Gene Expression; Inteins; Protein Engineering; Protein Splicing; Recombinant Proteins; Saccharomyces cerevisiae; Single-Chain Antibodies

2020
    The Journal of biological chemistry, 2020, 06-12, Volume: 295, Issue:24

    Topics: Binding Sites; Biotin; Cysteine; Glutathione; HeLa Cells; HSP70 Heat-Shock Proteins; Humans; Magnetic Resonance Spectroscopy; Mutant Proteins; Mutation; Peptides; Protein Stability; Protein Structure, Secondary; Protein Unfolding; Structure-Activity Relationship; Substrate Specificity

2020
Labeling Antibodies.
    Cold Spring Harbor protocols, 2020, 07-01, Volume: 2020, Issue:7

    Topics: Alzheimer Disease; Amyloid beta-Peptides; Antibodies; Biotin; Carbohydrates; Cross-Linking Reagents; Cysteine; Gold Colloid; Humans; Immunoconjugates; Isotope Labeling; Luminescent Proteins; Staining and Labeling; Sulfhydryl Compounds

2020
SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*.
    Chembiochem : a European journal of chemical biology, 2021, 05-14, Volume: 22, Issue:10

    Topics: Biotin; Cycloaddition Reaction; Cysteine; HEK293 Cells; Humans; Iodoacetamide; Ion Mobility Spectrometry; Peptides; Proteomics; Solid-Phase Synthesis Techniques

2021
A novel posttranslational modification of histone, H3 S-sulfhydration, is down-regulated in asthenozoospermic sperm.
    Journal of assisted reproduction and genetics, 2021, Volume: 38, Issue:12

    Topics: Amino Acid Sequence; Animals; Asthenozoospermia; Biotin; Cysteine; Gene Expression Regulation; Histones; Humans; Hydrogen Sulfide; Infertility; Male; Mice; Mice, Inbred C57BL; Mutation; Protein Processing, Post-Translational; Spermatogenesis; Spermatozoa; Sulfhydryl Compounds; Sulfides

2021
Crystal structure of the Cys-NO modified YopH tyrosine phosphatase.
    Biochimica et biophysica acta. Proteins and proteomics, 2022, 03-01, Volume: 1870, Issue:3

    Topics: Bacterial Outer Membrane Proteins; Biotin; Catalysis; Crystallography, X-Ray; Cysteine; Humans; Mass Spectrometry; Molecular Structure; Nitric Oxide; Oxidation-Reduction; Protein Tyrosine Phosphatases; Signal Transduction; Yersinia enterocolitica

2022
Biotin alleviates hepatic and intestinal inflammation and apoptosis induced by high dietary carbohydrate in juvenile turbot (Scophthalmus maximus L.).
    Fish & shellfish immunology, 2022, Volume: 130

    Topics: Animal Feed; Animals; Apoptosis; bcl-2-Associated X Protein; Biotin; Caspase 3; Cysteine; Diet; Dietary Carbohydrates; Dietary Supplements; Flatfishes; Inflammation; Interleukin-10; Interleukin-1beta; Interleukin-6; Interleukin-8; Liver; NF-kappa B; RNA, Messenger; Transforming Growth Factor beta; Transforming Growth Factors; Tumor Necrosis Factor-alpha

2022
Activity-Based Proteomic Identification of the
    Journal of agricultural and food chemistry, 2022, Nov-23, Volume: 70, Issue:46

    Topics: Antioxidants; Biotin; Breast Neoplasms; Cysteine; Disulfides; Female; Garlic; Humans; Proteomics; Sulfoxides

2022
Detection of Cysteine Sulfenic Acid on
    ACS chemical biology, 2023, 06-16, Volume: 18, Issue:6

    Topics: Biotin; Cysteine; Escherichia coli; Escherichia coli Proteins; Oxidation-Reduction; Sulfenic Acids

2023
An efficient site-selective, dual bioconjugation approach exploiting N-terminal cysteines as minimalistic handles to engineer tailored anti-HER2 affibody conjugates.
    European journal of medicinal chemistry, 2023, Nov-15, Volume: 260

    Topics: Biotin; Cell Line; Cysteine; Esters; Immunoconjugates

2023