cysteine has been researched along with 4-chloromercuribenzenesulfonate in 70 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 8 (11.43) | 18.7374 |
| 1990's | 23 (32.86) | 18.2507 |
| 2000's | 35 (50.00) | 29.6817 |
| 2010's | 3 (4.29) | 24.3611 |
| 2020's | 1 (1.43) | 2.80 |
| Authors | Studies |
|---|---|
| Disalvo, J | 1 |
| Danpure, CJ; Fyfe, DA | 1 |
| Kobayashi, GS; Maresca, B; Medoff, G; Schlessinger, D | 1 |
| Keller, K; Monden, I; Wellner, M | 2 |
| Archer, J; Badakere, S; Lowdell, M; Macey, M; MacLean, L; Perrett, D; Whelan, M | 1 |
| Carraway, RE; Doh, PS; Han, DS; Lee, CJ; Miller, TB; Park, CS | 1 |
| de Jong, MD; van Hoek, AN; van Os, CH | 1 |
| Duncan, G; Emptage, NJ; Hightower, KR | 1 |
| Low, PS; Thevenin, BJ; Willardson, BM | 1 |
| Solomon, AK; Toon, MR | 1 |
| Chasan, B; Lukacovic, MF; Solomon, AK; Toon, MR | 1 |
| Benga, G; Holmes, RP; Ionescu, M; Pop, VI; Popescu, O | 1 |
| Katiyar, SS; Sheikh, S | 2 |
| Kachar, B; Kalinec, F | 1 |
| Maloney, PC; Yan, RT | 1 |
| Katiyar, S; Pandey, A; Sheikh, S | 1 |
| Connolly, DT; Glenn, K; Heuvelman, D | 1 |
| Lebendiker, M; Schuldiner, S | 1 |
| Lee, H; Zhang, Y | 1 |
| Carnall, N; Carrington, M; Webb, H | 1 |
| Keller, K; Monden, I; Olsowski, A | 1 |
| Ashcroft, FM; Trapp, S; Tucker, SJ | 1 |
| Camps, M; Devés, R; Estévez, R; Hediger, MA; Palacín, M; Rojas, AM; Testar, X; Zorzano, A | 1 |
| Hu, LA; King, SC | 1 |
| Matsuzaki, S; Tamai, E; Tsuchiya, T; Weissborn, AC; Wilson, TH | 1 |
| Mensa-Wilmot, K; Rashid, MB; Russell, M | 1 |
| Akabas, MH; Williams, DB | 2 |
| Gangula, R; Krajewski, SJ; Pajor, AM; Sun, N | 1 |
| Bannister, JP; Main, MJ; Sivaprasadarao, A; Wray, D; Young, BA | 1 |
| Keller, K; Krause, G; Monden, I; Olsowski, A | 1 |
| Milligan, CJ; Wray, D | 1 |
| Bravo, DT; Keller, JE; Parsons, SM | 1 |
| Ding, PZ; Wilson, TH | 2 |
| Detmers, FJ; Konings, WN; Lanfermeijer, FC; Poolman, B | 1 |
| Bragg, PD; Hou, C | 1 |
| Cordeiro, MN; Diniz, CR; Gomez, MV; Kalapothakis, E; Prado, MA; Reis, HJ; Romano-Silva, MA | 1 |
| Hruz, PW; Mueckler, MM | 1 |
| Hall, JA; Maloney, PC | 1 |
| Lynch, JW; Seebungkert, B | 1 |
| Berridge, G; Callaghan, R; Ford, RC; Higgins, CF; Kerr, ID; Linton, KJ; Martin, C; Rosenberg, MF; Schmidlin, A; Velarde, G; Wooding, C | 1 |
| Makepeace, C; Mueckler, M | 3 |
| Ivanenkov, VV; Kirley, TL; Murphy, DM | 1 |
| Gordienko, DV; Greenwood, IA; Large, WA; Leblanc, N | 1 |
| Mensa-Wilmot, K; Rashid, MB; Stanton, JD | 1 |
| Elhammer, AP; Kézdy, FJ; Kurosaka, A; Tenno, M; Toba, S | 1 |
| Ding, Z; Dorsam, RT; Jin, J; Kim, S; Kunapuli, SP | 1 |
| Keller, JE; Parsons, SM | 1 |
| Busch, A; Fann, MC; Maloney, PC | 1 |
| Ding, PZ | 2 |
| Heinze, M; Keller, K; Monden, I | 1 |
| Kuze, K; Tanaka, K; You, G; Zhou, F | 1 |
| Makepeace, C; Mueckler, M; Roach, W | 1 |
| Davies, AJ; Fox, JE; Halestrap, AP; Manoharan, C; Meredith, D; Wilson, MC | 1 |
| Aller, SG; Dawson, DC; Decker, SE; Forrest, JN; Mehr, AP; Sirota, JC; Weber, GJ | 1 |
| Bevensee, MO; McAlear, SD | 1 |
| Asaka, J; Inui, K; Katsura, T; Terada, T; Tsuda, M | 1 |
| Baldwin, SA; Cass, CE; Karpinski, E; Lin, CC; Ng, AM; Slugoski, MD; Smith, KM; Yao, SY; Young, JD; Zhang, J | 1 |
| Chiang, WC; Knowles, AF | 1 |
| Baldwin, SA; Cass, CE; Lin, CC; Mulinta, R; Ng, AML; Slugoski, MD; Yao, SYM; Young, JD | 1 |
| Bailey, MA; Devor, DC; Grabe, M | 1 |
| Akabas, MH; Bali, M; Parikh, RB | 1 |
| Groeneweg, S; Lima de Souza, EC; Peeters, RP; Visser, TJ; Visser, WE | 1 |
| Beitz, E; Geistlinger, K; Köpnick, AL | 1 |
70 other study(ies) available for cysteine and 4-chloromercuribenzenesulfonate
| Article | Year |
|---|---|
Differential effects of p-chloromercuriphenyl sulfonate on arterial responses to epinephrine and serotonin.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Arteries; Cysteine; Dose-Response Relationship, Drug; Epinephrine; Female; In Vitro Techniques; Isoproterenol; Male; Muscle Contraction; Muscle, Smooth; Phentolamine; Phenylmercury Compounds; Rats; Serotonin; Serotonin Antagonists; Time Factors; Vasomotor System | 1976 |
Inability of organomercurials to inhibit the reaction between aurothiomalate and thiols.
Topics: 4-Chloromercuribenzenesulfonate; Chemical Phenomena; Chemistry; Chloromercuribenzoates; Cysteine; Ethylmaleimide; Gold Sodium Thiomalate; Phenylmercury Compounds; Serum Albumin | 1976 |
Regulation of dimorphism in the pathogenic fungus Histoplasma capsulatum.
Topics: 4-Chloromercuribenzenesulfonate; Biological Transport; Cell Differentiation; Cyclic AMP; Cysteine; Cystine; Dithiothreitol; Histoplasma; Oxidation-Reduction; Oxygen Consumption; Temperature | 1977 |
The differential role of Cys-421 and Cys-429 of the Glut1 glucose transporter in transport inhibition by p-chloromercuribenzenesulfonic acid (pCMBS) or cytochalasin B (CB).
Topics: 4-Chloromercuribenzenesulfonate; Animals; Biological Transport; Cysteine; Cytochalasin B; Glucose; Glucose Transporter Type 1; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Xenopus | 1992 |
Sulphydryl reactivity of the HLA-B27 epitope: accessibility of the free cysteine studied by flow cytometry.
Topics: 4-Chloromercuribenzenesulfonate; Arthritis; Cysteine; Enzyme-Linked Immunosorbent Assay; Epitopes; Flow Cytometry; HLA-B27 Antigen; Humans | 1992 |
Cellular mechanism of stimulation of renin secretion by the mercurial diuretic mersalyl.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Chlorides; Cysteine; Ethacrynic Acid; In Vitro Techniques; Kidney Cortex; Mersalyl; Potassium; Rabbits; Renin; Sodium; Stimulation, Chemical | 1991 |
Effects of dimethylsulfoxide and mercurial sulfhydryl reagents on water and solute permeability of rat kidney brush border membranes.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Cell Membrane Permeability; Cysteine; Dimethyl Sulfoxide; Dithiothreitol; Kidney; Kinetics; Mannitol; Mercury; Microvilli; Osmosis; Rats; Sodium Chloride; Sulfhydryl Reagents; Urea; Water | 1990 |
p-chloro-mercuriphenyl sulphonate activates a quinine-sensitive potassium conductance in frog lens.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Cysteine; Dose-Response Relationship, Drug; Electric Conductivity; In Vitro Techniques; Ion Channels; Lens, Crystalline; Membrane Potentials; Phenylmercury Compounds; Potassium; Quinine; Rana pipiens | 1988 |
The redox state of cysteines 201 and 317 of the erythrocyte anion exchanger is critical for ankyrin binding.
Topics: 4-Chloromercuribenzenesulfonate; Anion Exchange Protein 1, Erythrocyte; Anion Transport Proteins; Ankyrins; Blood Proteins; Carrier Proteins; Cysteine; Erythrocyte Membrane; Ethylmaleimide; Glutathione; Humans; Iodoacetamide; Kinetics; Membrane Proteins; Molecular Weight; Oxidation-Reduction; Phenanthrolines | 1989 |
Control of red cell urea and water permeability by sulfhydryl reagents.
Topics: 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; 4-Chloromercuribenzenesulfonate; Anion Exchange Protein 1, Erythrocyte; Body Water; Cell Membrane Permeability; Cysteine; Erythrocyte Membrane; Erythrocytes; Humans; Kinetics; Protein Conformation; Stilbenes; Sulfhydryl Reagents; Urea | 1986 |
Effect of thiourea on PCMBS inhibition of osmotic water transport in human red cells.
Topics: 4-Chloromercuribenzenesulfonate; Anion Exchange Protein 1, Erythrocyte; Biological Transport; Cysteine; Drug Interactions; Erythrocyte Membrane; Humans; Kinetics; Osmosis; Phenylmercury Compounds; Sulfhydryl Compounds; Thiourea; Water | 1984 |
Irreversible inhibition of water transport in erythrocytes by fluoresceinmercuric acetate.
Topics: 4-Chloromercuribenzenesulfonate; Biological Transport; Chemical Phenomena; Chemistry; Cysteine; Diffusion; Erythrocyte Membrane; Erythrocytes; Fluoresceins; Humans; Models, Biological; Organomercury Compounds; Water | 1982 |
Investigation of the nature of o-phthalaldehyde reaction with octopine dehydrogenase.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Oxidoreductases; Arginine; Binding Sites; Cysteine; Dithiothreitol; Enzyme Activation; Kinetics; Lysine; Mercaptoethanol; NAD; o-Phthalaldehyde; Protein Conformation; Pyruvates; Spectrometry, Fluorescence; Spectrophotometry; Substrate Specificity; Valine | 1994 |
The role of cysteine residues in glucose-transporter-GLUT1-mediated transport and transport inhibition.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Biological Transport; Cells, Cultured; Cysteine; DNA, Complementary; Glucose Transporter Type 1; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Xenopus | 1994 |
Inhibition of outer hair cell electromotility by sulfhydryl specific reagents.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Cell Movement; Cysteine; Cystine; Diamide; Electric Stimulation; Ethacrynic Acid; Ethylmaleimide; Guinea Pigs; Hair Cells, Auditory, Outer; Membrane Proteins; Mersalyl; Nerve Tissue Proteins; Protein Conformation; Sulfhydryl Reagents | 1993 |
Involvement of different cysteines in the inactivation of octopine dehydrogenase by p-chloromercuricphenyl sulfonic acid and o-phthalaldehyde.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Oxidoreductases; Binding Sites; Cysteine; Dithiothreitol; Indoles; Lysine; NAD; o-Phthalaldehyde; Spectrometry, Fluorescence | 1993 |
Identification of a residue in the translocation pathway of a membrane carrier.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Bacterial Proteins; Base Sequence; Carrier Proteins; Cell Membrane; Cysteine; Escherichia coli; Escherichia coli Proteins; Glucose-6-Phosphate; Glucosephosphates; Kinetics; Models, Structural; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Oligodeoxyribonucleotides; Protein Structure, Secondary | 1993 |
Identification of cysteine and lysine residues present at the active site of beef liver glutamate dehydrogenase by o-phthalaldehyde.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Binding Sites; Cattle; Cysteine; Enzyme Inhibitors; Glutamate Dehydrogenase; Indoles; Ketoglutaric Acids; Kinetics; Liver; Lysine; Mercaptoethanol; Models, Chemical; NAD; o-Phthalaldehyde; Spectrometry, Fluorescence | 1996 |
Inactivation of cholesteryl ester transfer protein by cysteine modification.
Topics: 4-Chloromercuribenzenesulfonate; Alkylating Agents; Animals; Azides; Carrier Proteins; Cell Line; Cholesterol Ester Transfer Proteins; Cholesterol Esters; Cricetinae; Cysteine; Disulfides; Dithiothreitol; Ethylmaleimide; Glycoproteins; Humans; Iodoacetamide; Kinetics; Pyridines; Recombinant Proteins; Structure-Activity Relationship; Sulfhydryl Reagents; Transfection | 1996 |
Identification of residues in the translocation pathway of EmrE, a multidrug antiporter from Escherichia coli.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Antiporters; Bacterial Proteins; Biological Transport; Carrier Proteins; Chloromercuribenzoates; Cysteine; Escherichia coli; Escherichia coli Proteins; Membrane Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; p-Chloromercuribenzoic Acid; Protein Structure, Secondary; Sulfhydryl Reagents | 1996 |
Site-directed mutagenesis of the cysteine residues in the Pichia stipitis xylose reductase.
Topics: 4-Chloromercuribenzenesulfonate; Aldehyde Reductase; Binding Sites; Cloning, Molecular; Coenzymes; Cysteine; Enzyme Inhibitors; Mutagenesis, Site-Directed; NAD; NADP; Pichia; Plasmids; Polymerase Chain Reaction; Recombinant Proteins; Recombination, Genetic; Serine; Xylose | 1997 |
Mutagenesis study of the glycosylphosphatidylinositol phospholipase C of Trypanosoma brucei.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Animals; Bacillus cereus; Binding Sites; Cysteine; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Escherichia coli; Glycosylphosphatidylinositol Diacylglycerol-Lyase; Histidine; Listeria monocytogenes; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphatidylinositol Diacylglycerol-Lyase; Phosphoinositide Phospholipase C; Recombinant Proteins; Sequence Alignment; Sulfhydryl Reagents; Trypanosoma brucei brucei; Type C Phospholipases | 1997 |
Cysteine-scanning mutagenesis of flanking regions at the boundary between external loop I or IV and transmembrane segment II or VII in the GLUT1 glucose transporter.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Amino Acid Substitution; Animals; Biological Transport; Cysteine; Glucose; Glucose Transporter Type 1; Humans; Membrane Proteins; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Serine; Xenopus laevis | 1998 |
Mechanism of ATP-sensitive K channel inhibition by sulfhydryl modification.
Topics: 4-Chloromercuribenzenesulfonate; Adenosine Triphosphate; Animals; Cysteine; Ion Channel Gating; Mice; Mutagenesis, Site-Directed; Oocytes; Potassium Channels; Potassium Channels, Inwardly Rectifying; Sulfhydryl Reagents; Xenopus laevis | 1998 |
The amino acid transport system y+L/4F2hc is a heteromultimeric complex.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acids; Animals; Antigens, CD; Biological Transport; Carrier Proteins; Cysteine; Fusion Regulatory Protein-1; Gene Expression Regulation; Humans; Macromolecular Substances; Mercaptoethanol; Mercuric Chloride; Mutagenesis, Site-Directed; Oocytes; p-Chloromercuribenzoic Acid; Protein Conformation; Protein Processing, Post-Translational; Recombinant Fusion Proteins; RNA; Xenopus laevis | 1998 |
Identification of the amine-polyamine-choline transporter superfamily 'consensus amphipathic region' as the target for inactivation of the Escherichia coli GABA transporter GabP by thiol modification reagents. Role of Cys-300 in restoring thiol sensitivit
Topics: 4-Chloromercuribenzenesulfonate; Amines; Amino Acid Substitution; Biological Transport; Cell Membrane Permeability; Choline; Consensus Sequence; Cysteine; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Kinetics; Membrane Proteins; Membrane Transport Modulators; Membrane Transport Proteins; Mesylates; Models, Biological; Organic Anion Transporters; Polyamines; Protein Conformation; Substrate Specificity; Sulfhydryl Compounds; Sulfhydryl Reagents | 1999 |
Melibiose carrier of Escherichia coli: use of cysteine mutagenesis to identify the amino acids on the hydrophilic face of transmembrane helix 2.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Biological Transport, Active; Catalytic Domain; Cysteine; Escherichia coli; Melibiose; Membrane Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Sulfhydryl Reagents; Symporters | 1999 |
Roles of Gln81 and Cys80 in catalysis by glycosylphosphatidylinositol-phospholipase C from Trypanosoma brucei.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Animals; Base Sequence; Catalytic Domain; Cysteine; DNA Primers; Enzyme Inhibitors; Escherichia coli; Glutamine; Kinetics; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphatidylinositol Diacylglycerol-Lyase; Recombinant Proteins; Sequence Homology, Amino Acid; Trypanosoma brucei brucei; Type C Phospholipases | 1999 |
Gamma-aminobutyric acid increases the water accessibility of M3 membrane-spanning segment residues in gamma-aminobutyric acid type A receptors.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Substitution; Animals; Cell Membrane; Cysteine; gamma-Aminobutyric Acid; Hydrophobic and Hydrophilic Interactions; Ion Channel Gating; Mesylates; Mutation; Protein Binding; Protein Conformation; Rats; Receptors, GABA-A; Sulfhydryl Compounds; Surface Properties; Water | 1999 |
Cysteine residues in the Na+/dicarboxylate co-transporter, NaDC-1.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Base Sequence; Binding Sites; Carrier Proteins; Cysteine; Dicarboxylic Acid Transporters; DNA Primers; Female; In Vitro Techniques; Membrane Proteins; Mutagenesis, Site-Directed; Oocytes; Organic Anion Transporters, Sodium-Dependent; Protein Structure, Secondary; Recombinant Proteins; Sulfhydryl Reagents; Symporters; Xenopus laevis | 1999 |
The effects of oxidizing and cysteine-reactive reagents on the inward rectifier potassium channels Kir2.3 and Kir1.1.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Cysteine; Extracellular Space; Female; Hydrogen Peroxide; Mutation; Oocytes; Oxidants; Potassium Channel Blockers; Potassium Channels; Potassium Channels, Inwardly Rectifying; Thimerosal; Xenopus | 1999 |
Cysteine scanning mutagenesis of helices 2 and 7 in GLUT1 identifies an exofacial cleft in both transmembrane segments.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Substitution; Computer Simulation; Cysteine; Ethylmaleimide; Glucose Transporter Type 1; Glutamine; Humans; Isoleucine; Membrane Proteins; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Structure, Secondary; Sulfhydryl Reagents | 2000 |
Local movement in the S2 region of the voltage-gated potassium channel hKv2.1 studied using cysteine mutagenesis.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Base Sequence; Biophysical Phenomena; Biophysics; Cysteine; Delayed Rectifier Potassium Channels; DNA Primers; Escherichia coli; Female; Humans; In Vitro Techniques; Mutagenesis, Site-Directed; Oocytes; Potassium Channels; Potassium Channels, Voltage-Gated; Recombinant Proteins; Sulfhydryl Reagents; Xenopus | 2000 |
Modification of cysteines reveals linkage to acetylcholine and vesamicol binding sites in the vesicular acetylcholine transporter of Torpedo californica.
Topics: 4-Chloromercuribenzenesulfonate; Acetylcholine; Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Binding, Competitive; Carrier Proteins; Cysteine; Cytoplasm; Dose-Response Relationship, Drug; Glutathione; Membrane Transport Proteins; Methyl Methanesulfonate; Methylmercury Compounds; Molecular Sequence Data; Neuromuscular Depolarizing Agents; Organomercury Compounds; Phenylmercury Compounds; Piperidines; Protein Structure, Tertiary; Torpedo; Vesicular Acetylcholine Transport Proteins; Vesicular Transport Proteins | 2000 |
The melibiose carrier of Escherichia coli: cysteine substitutions for individual residues in helix XI.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Substitution; Biological Transport; Cell Membrane; Cysteine; Escherichia coli; Lithium; Melibiose; Membrane Transport Proteins; Mutagenesis, Site-Directed; Phenotype; Sodium; Symporters | 2000 |
On the binding mechanism of the peptide receptor of the oligopeptide transport system of Lactococcus lactis.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Aza Compounds; Bacterial Proteins; Binding Sites; Biological Transport; Cysteine; Fluorescent Dyes; Lactococcus lactis; Membrane Transport Proteins; Molecular Structure; Naphthalenesulfonates; Oligopeptides; Protein Binding; Receptors, Cell Surface; Spectrometry, Fluorescence; Tryptophan | 2000 |
The presence of an aqueous cavity in the proton-pumping pathway of the pyridine nucleotide transhydrogenase of Escherichia coli is suggested by the reaction of the enzyme with sulfhydryl inhibitors.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Cell Membrane; Cysteine; Electrophoresis, Polyacrylamide Gel; Enzyme Inhibitors; Escherichia coli; Ethylmaleimide; Molecular Sequence Data; Mutagenesis, Site-Directed; NADP Transhydrogenases; Plasmids; Protein Structure, Tertiary; Protons; Sulfhydryl Reagents; Time Factors; Water | 2000 |
Inhibition of glutamate uptake by Tx3-4 is dependent on the redox state of cysteine residues.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Biological Transport; Cysteine; Dithionitrobenzoic Acid; Dithiothreitol; Glutamic Acid; Hippocampus; Neuropeptides; Neurotoxins; Oxidation-Reduction; Rats; Rats, Wistar; Spider Venoms; Synaptosomes | 2000 |
Cysteine-scanning mutagenesis of transmembrane segment 11 of the GLUT1 facilitative glucose transporter.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Substitution; Animals; Biological Transport; Cysteine; Deoxyglucose; Genetic Vectors; Glucose; Glucose Transporter Type 1; Humans; Membrane Proteins; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Oocytes; Peptide Fragments; Point Mutation; Sulfhydryl Reagents; Xenopus laevis | 2000 |
Benzodiazepines induce a conformational change in the region of the gamma-aminobutyric acid type A receptor alpha(1)-subunit M3 membrane-spanning segment.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Substitution; Animals; Benzodiazepines; Carbolines; Cysteine; Diazepam; Flumazenil; GABA Modulators; Membrane Proteins; Oocytes; Protein Conformation; Rats; Receptors, GABA-A; Xenopus laevis | 2000 |
Cysteine mutagenesis of the amino acid residues of transmembrane helix I in the melibiose carrier of Escherichia coli.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Amino Acid Substitution; Biological Transport; Cations, Monovalent; Cell Membrane; Cysteine; Escherichia coli; Immune Sera; Melibiose; Membrane Transport Modulators; Membrane Transport Proteins; Molecular Sequence Data; Mutagenesis, Site-Directed; Peptide Fragments; Protein Structure, Secondary; Symporters | 2001 |
Transmembrane segment 11 of UhpT, the sugar phosphate carrier of Escherichia coli, is an alpha-helix that carries determinants of substrate selectivity.
Topics: 4-Chloromercuribenzenesulfonate; Bacterial Proteins; Carrier Proteins; Cysteine; Escherichia coli; Escherichia coli Proteins; Kinetics; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Pentose Phosphate Pathway; Protein Binding; Protein Structure, Secondary; Structure-Activity Relationship | 2001 |
A common inhibitory binding site for zinc and odorants at the voltage-gated K(+) channel of rat olfactory receptor neurons.
Topics: 4-Chloromercuribenzenesulfonate; Acetophenones; Animals; Binding Sites; Carbon Monoxide; Cells, Cultured; Cyclohexenes; Cysteine; Diethyl Pyrocarbonate; Dose-Response Relationship, Drug; Histidine; Limonene; Membrane Potentials; Mesylates; Neural Inhibition; Nitric Oxide; Olfactory Receptor Neurons; Patch-Clamp Techniques; Pentanols; Potassium; Potassium Channels; Rats; Receptors, Odorant; Smell; Sulfhydryl Reagents; Terpenes; Zinc | 2001 |
Repacking of the transmembrane domains of P-glycoprotein during the transport ATPase cycle.
Topics: 4-Chloromercuribenzenesulfonate; Adenosine Diphosphate; Adenosine Triphosphatases; Adenosine Triphosphate; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Binding Sites; Catalysis; Cell Line; CHO Cells; Cricetinae; Cricetulus; Cryoelectron Microscopy; Crystallization; Crystallography, X-Ray; Cysteine; Drug Resistance, Multiple; Enzyme Inhibitors; Hydrolysis; Insecta; Models, Molecular; Mutagenesis, Site-Directed; p-Chloromercuribenzoic Acid; Protein Binding; Protein Conformation; Protein Structure, Tertiary; Protein Transport; Recombinant Fusion Proteins; Structure-Activity Relationship | 2001 |
Analysis of transmembrane segment 10 of the Glut1 glucose transporter by cysteine-scanning mutagenesis and substituted cysteine accessibility.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Amino Acid Substitution; Animals; Cell Membrane; Cysteine; DNA, Complementary; Female; Glucose; Glucose Transporter Type 1; Humans; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Oocytes; Protein Structure, Secondary; Recombinant Proteins; Xenopus laevis | 2002 |
Identification of cysteine residues responsible for oxidative cross-linking and chemical inhibition of human nucleoside-triphosphate diphosphohydrolase 3.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Brain; Cross-Linking Reagents; Cysteine; Humans; Kinetics; Macromolecular Substances; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Phosphodiesterase Inhibitors; Protein Conformation; Pyrophosphatases; Recombinant Proteins; Sulfhydryl Compounds | 2002 |
Modulation of ICl(Ca) in vascular smooth muscle cells by oxidizing and cysteine-reactive reagents.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Calcium; Chloride Channels; Cysteine; Diamide; Dithiothreitol; Hydrogen Peroxide; In Vitro Techniques; Membrane Potentials; Muscle, Smooth, Vascular; Oxidants; Oxidation-Reduction; Patch-Clamp Techniques; Portal Vein; Rabbits; Sulfhydryl Reagents | 2002 |
Cysteine-less glycosylphosphatidylinositol-specific phospholipase C is inhibited competitively by a thiol reagent: evidence for glyco-mimicry by p-chloromercuriphenylsulphonate.
Topics: 4-Chloromercuribenzenesulfonate; Allosteric Site; Animals; Bacillus cereus; Binding Sites; Binding, Competitive; Catalysis; Chelating Agents; Cysteine; Dose-Response Relationship, Drug; Edetic Acid; Enzyme Inhibitors; Glycosylphosphatidylinositols; Kinetics; Mercaptoethanol; Mercury; Protein Binding; Substrate Specificity; Trypanosoma brucei brucei; Type C Phospholipases | 2002 |
Identification of two cysteine residues involved in the binding of UDP-GalNAc to UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1).
Topics: 4-Chloromercuribenzenesulfonate; Animals; Binding Sites; Cattle; COS Cells; Cysteine; DNA Primers; Enzyme Activation; Gene Deletion; Glycosylation; Humans; Isoflurophate; Kinetics; Mutagenesis, Site-Directed; N-Acetylgalactosaminyltransferases; Polymerase Chain Reaction; Polypeptide N-acetylgalactosaminyltransferase; Rats; Recombinant Proteins; Serine; Transfection; Uridine Diphosphate; Uridine Diphosphate N-Acetylgalactosamine | 2002 |
Inactivation of the human P2Y12 receptor by thiol reagents requires interaction with both extracellular cysteine residues, Cys17 and Cys270.
Topics: 4-Chloromercuribenzenesulfonate; Adenosine Diphosphate; Amino Acid Sequence; Animals; Base Sequence; Binding Sites; CHO Cells; Cricetinae; Cyclic AMP; Cysteine; DNA Primers; Humans; Inositol Phosphates; Kinetics; Membrane Proteins; Molecular Sequence Data; Purinergic P2 Receptor Antagonists; Receptors, Purinergic P2; Receptors, Purinergic P2Y1; Receptors, Purinergic P2Y12; Recombinant Proteins; Sequence Alignment; Sequence Homology, Amino Acid; Sulfhydryl Compounds; Transfection | 2003 |
Diffusion pathways to critical cysteines in the vesicular acetylcholine transporter of Torpedo.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Carrier Proteins; Cysteine; Diffusion; Ions; Membrane Transport Proteins; Organomercury Compounds; Osmolar Concentration; p-Chloromercuribenzoic Acid; Permeability; Phenylmercury Compounds; Sodium Chloride; Synaptic Vesicles; Torpedo; Vesicular Acetylcholine Transport Proteins; Vesicular Transport Proteins | 2003 |
Functional characterization of cysteine residues in GlpT, the glycerol 3-phosphate transporter of Escherichia coli.
Topics: 4-Chloromercuribenzenesulfonate; Biological Transport; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Histidine; Immunoblotting; Membrane Transport Proteins; Mesylates; Mutagenesis, Site-Directed; Proteolipids; Structure-Activity Relationship; Substrate Specificity; Sulfhydryl Reagents | 2003 |
An investigation of cysteine mutants on the cytoplasmic loop X/XI in the melibiose transporter of Escherichia coli by using thiol reagents: implication of structural conservation of charged residues.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acids; Animals; Biological Transport; Cells, Cultured; Cysteine; Escherichia coli Proteins; Iodoacetic Acid; Melibiose; Mesylates; Mutation; Protein Structure, Secondary; Sulfhydryl Reagents; Symporters | 2003 |
Analysis of transmembrane segment 8 of the GLUT1 glucose transporter by cysteine-scanning mutagenesis and substituted cysteine accessibility.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Binding Sites; Biological Transport; Blotting, Western; Cell Membrane; Cysteine; Deoxyglucose; DNA, Complementary; Glucose; Glucose Transport Proteins, Facilitative; Humans; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis; Mutation; Oocytes; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Xenopus laevis | 2004 |
Cysteine-scanning mutagenesis of transmembrane segment 1 of glucose transporter GLUT1: extracellular accessibility of helix positions.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Substitution; Animals; Cell Membrane; Cell Membrane Permeability; Cysteine; Ethylmaleimide; Extracellular Fluid; Glucose; Glucose Transporter Type 1; Humans; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Oocytes; Peptide Fragments; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Transport; Sulfhydryl Reagents; Xenopus | 2004 |
Loop X/XI, the largest cytoplasmic loop in the membrane-bound melibiose carrier of Escherichia coli, is a functional re-entrant loop.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Cysteine; Cytoplasm; Escherichia coli; Melibiose; Molecular Sequence Data; Periplasm; Plasmids; Sulfhydryl Reagents; Symporters | 2004 |
Cysteine residues in the organic anion transporter mOAT1.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Substitution; Animals; Biological Transport; Cysteine; HeLa Cells; Humans; Inactivation, Metabolic; Kidney; Membrane Proteins; Mice; Models, Molecular; Mutagenesis, Site-Directed; Organic Anion Transport Protein 1; Organic Anion Transporters; p-Aminohippuric Acid; Protein Structure, Secondary; Protein Transport; Recombinant Fusion Proteins; Structure-Activity Relationship | 2004 |
Transmembrane segment 3 of the Glut1 glucose transporter is an outer helix.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Binding Sites; Catalysis; Cysteine; Cystine; Deoxyglucose; DNA, Complementary; Enzyme Inhibitors; Glucose; Glucose Transporter Type 1; Humans; Models, Molecular; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Structure, Secondary; RNA, Messenger; Substrate Specificity; Sulfhydryl Reagents; Xenopus laevis | 2004 |
Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70).
Topics: 4-Chloromercuribenzenesulfonate; Animals; Antigens, CD; Basigin; Cell Membrane; Cells, Cultured; Cysteine; Erythrocytes; Glycoproteins; Humans; Membrane Glycoproteins; Membrane Proteins; Molecular Chaperones; Monocarboxylic Acid Transporters; Muscle Proteins; Mutagenesis, Site-Directed; Organomercury Compounds; Protein Isoforms; Protein Transport; Rabbits; Rats; Symporters; Transfection; Xenopus | 2005 |
Mercury and zinc differentially inhibit shark and human CFTR orthologues: involvement of shark cysteine 102.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Cell Membrane; Cysteine; Cystic Fibrosis Transmembrane Conductance Regulator; Electric Conductivity; Humans; Mercuric Chloride; Mutagenesis, Site-Directed; Mutation; Oocytes; Sharks; Species Specificity; Xenopus laevis; Zinc Acetate | 2006 |
A cysteine-scanning mutagenesis study of transmembrane domain 8 of the electrogenic sodium/bicarbonate cotransporter NBCe1.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Biological Transport; Cysteine; Female; Gene Expression Regulation; Mesylates; Microelectrodes; Mutagenesis; Oocytes; Patch-Clamp Techniques; Protein Structure, Tertiary; Rats; RNA, Complementary; Sodium-Bicarbonate Symporters; Sulfhydryl Reagents; Xenopus laevis | 2006 |
Transmembrane segment 12 of the Glut1 glucose transporter is an outer helix and is not directly involved in the transport mechanism.
Topics: 4-Chloromercuribenzenesulfonate; Animals; Binding Sites; Biological Transport; Cysteine; Enzyme Inhibitors; Female; Gene Expression Regulation; Glucose Transport Proteins, Facilitative; Glucose Transporter Type 1; Mutation; Oocytes; Point Mutation; Protein Structure, Secondary; Protein Structure, Tertiary; Xenopus laevis | 2006 |
Identification of essential histidine and cysteine residues of the H+/organic cation antiporter multidrug and toxin extrusion (MATE).
Topics: 4-Chloromercuribenzenesulfonate; Animals; Antiporters; Biological Transport; Cysteine; Diethyl Pyrocarbonate; Gene Expression; Histidine; Humans; Mutagenesis, Site-Directed; Organic Cation Transport Proteins; Rats; Tetraethylammonium; Toxins, Biological | 2007 |
A proton-mediated conformational shift identifies a mobile pore-lining cysteine residue (Cys-561) in human concentrative nucleoside transporter 3.
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Sequence; Animals; Cysteine; Electrophysiology; Humans; Membrane Transport Proteins; Mesylates; Models, Molecular; Molecular Sequence Data; Mutation; Oocytes; Patch-Clamp Techniques; Protein Structure, Tertiary; Protons; Sequence Alignment; Uridine; Xenopus laevis | 2008 |
Inhibition of human NTPDase 2 by modification of an intramembrane cysteine by p-chloromercuriphenylsulfonate and oxidative cross-linking of the transmembrane domains.
Topics: 4-Chloromercuribenzenesulfonate; Adenosine Triphosphatases; Amino Acid Sequence; Cell Line; Cell Membrane; Cysteine; Gene Expression Regulation; Humans; Molecular Sequence Data; Mutation; Oxidation-Reduction; Protein Structure, Tertiary | 2008 |
Substituted cysteine accessibility method analysis of human concentrative nucleoside transporter hCNT3 reveals a novel discontinuous region of functional importance within the CNT family motif (G/A)XKX3NEFVA(Y/M/F).
Topics: 4-Chloromercuribenzenesulfonate; Amino Acid Motifs; Amino Acid Sequence; Amino Acid Substitution; Animals; Binding Sites; Cysteine; Female; Humans; In Vitro Techniques; Membrane Transport Proteins; Models, Molecular; Mutagenesis, Site-Directed; Nucleosides; Oocytes; Protein Structure, Secondary; Recombinant Proteins; Uridine; Xenopus laevis | 2009 |
Characterization of the PCMBS-dependent modification of KCa3.1 channel gating.
Topics: 4-Chloromercuribenzenesulfonate; Calcium; Cells, Cultured; Cysteine; Humans; Intermediate-Conductance Calcium-Activated Potassium Channels; Ion Channel Gating; Kinetics; Leucine; Structure-Activity Relationship | 2010 |
Structure of the M2 transmembrane segment of GLIC, a prokaryotic Cys loop receptor homologue from Gloeobacter violaceus, probed by substituted cysteine accessibility.
Topics: 4-Chloromercuribenzenesulfonate; Crystallography, X-Ray; Cyanobacteria; Cysteine; Hydrogen-Ion Concentration; Ion Channels; Membrane Proteins; Mutation; Neurotransmitter Agents; Protein Structure, Secondary; Protein Structure, Tertiary; Protons; Receptors, GABA; Receptors, Nicotinic; Receptors, Serotonin, 5-HT3 | 2011 |
Importance of cysteine residues in the thyroid hormone transporter MCT8.
Topics: 4-Chloromercuribenzenesulfonate; Alanine; Amino Acid Substitution; Cell Line, Tumor; Cysteine; Dithiothreitol; Dose-Response Relationship, Drug; Humans; Monocarboxylic Acid Transporters; Mutagenesis, Site-Directed; Point Mutation; Sulfhydryl Reagents; Symporters; Time Factors; Transfection | 2013 |
Cysteine 159 delineates a hinge region of the alternating access monocarboxylate transporter 1 and is targeted by cysteine-modifying inhibitors.
Topics: 4-Chloromercuribenzenesulfonate; Basigin; Cysteine; Enzyme Inhibitors; Humans; Monocarboxylic Acid Transporters; Protein Conformation; Small Molecule Libraries; Symporters | 2021 |