cyhalothrin and tebuconazole

The aim of this study was to investigate pesticide residues on Sultana Seedless Grapes harvested at different times and consequently evaluate the risk. Analyses were performed with the Quick-Easy-Cheap-Efficient-Rugged-Safe (QuEChERS)-liquid chromatography/tandem mass spectrometry (LC-MS/MS) procedure. Pesticide-free grapes were spiked at 3 levels. Chlorpyrifos-methyl, lambda-cyhalothrin and tebuconazole detection limits were 20, 10 and 1 µg kg

Topics: Chlorpyrifos; Chromatography, Liquid; Farmers; Food Contamination; Humans; Nitriles; Pesticide Residues; Pesticides; Pyrethrins; Tandem Mass Spectrometry; Triazoles; Vitis

Although jujube is a minor crop and very few pesticides are registered on it, the application of pesticides during the growth stage of jujube is inevitable to control the pests or diseases. This situation has led to pesticide misuse. A modified quick, easy, cheap, effective, rugged, and safe method using a novel sorbent, multiwalled carbon nanotubes, as a dispersive solid-phase extraction sorbent combined with gas chromatography with mass spectrometry, was developed for the determination of 16 pesticides in jujube. Under the optimized conditions, recoveries of 76.7-112.4% were obtained for the target analytes at three spiked concentration levels. The relative standard deviations ranged from 1.2 to 12.3%. Limits of detection and limits of quantification for 16 pesticides ranged from 1 to 10 and 3 to 30 μg/kg, respectively. The residues of chlorpyrifos, hexaconazole, tebuconazole, and cyhalothrin were detected from samples obtained from the market.

Topics: Adsorption; Chemistry, Pharmaceutical; Chlorpyrifos; Food Analysis; Food Contamination; Fruit; Gas Chromatography-Mass Spectrometry; Limit of Detection; Nanotechnology; Nanotubes, Carbon; Nitriles; Pesticide Residues; Pesticides; Pyrethrins; Solid Phase Extraction; Triazoles; Ziziphus

Changes in microbiological properties of a sandy loam soil in response to the addition of different concentrations of fungicide tebuconazole and pyrethroid insecticide lambda-cyhalothrin were assessed under laboratory conditions. To ascertain these changes, the potentially active soil microbial biomass, concentrations of ammonium and nitrate ions, numbers of total culturable bacteria, fungi, nitrogen-fixing bacteria, nitrifying and denitrifying bacteria were determined. Substrate-induced respiration (SIR) increased with time in both control (ranged from 13.7 to 23.7 mg/O(2)/kg(-1)/dry soil/h(-1)) and pesticide treated soil portions. For both pesticides, SIR values ranged from 12-13 to 23-25 mg/O(2)/kg(-1)/dry soil/h(-1) on days 1 and 28, respectively. Also, concentrations of nitrate and ammonium ions, numbers of total culturable bacteria, denitrifying bacteria, nitrogen-fixing bacteria (for the insecticide) and fungi (for the insecticide) were either unaffected or even stimulated by the pesticide treatments. The adverse impacts of the pesticides were observed for nitrate concentrations (on days 1 or 7), numbers of nitrifying bacteria (on day 1), denitrifying bacteria (for the insecticide on days 1 and 14), nitrogen-fixing bacteria (for tebuconazole on day 1) as well as numbers of fungi in tebuconazole-treated soil (on days 1 and 14).

Topics: Bacteria; Humans; Nitriles; Oxygen Consumption; Pesticides; Pyrethrins; Soil Microbiology; Species Specificity; Triazoles

Applications of the fungicide Matador to winter wheat (Slejpner) inoculated at earing with Fusarium culmorum resulted in a considerable decrease in the incidence of Fusarium headblight. In the study presented here, subsequent mycotoxin analyses by selected ion monitoring gas chromatography-mass spectrometry revealed higher levels of nivalenol (NIV) in samples singly treated with Matador at 1 L/ha. Amounts as high as 2432 micrograms NIV/kg and 860 micrograms NIV/kg, representing a 16- or six-fold increase as compared to controls, were found in wheat treated with the fungicide 3 h before or 24 h after inoculation of the grain with F. culmorum, respectively. In parallel, higher cytotoxic activities of these samples were obtained when testing crude sample extracts in the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-cell culture bioassay using swine kidney monolayers as target cells. It is concluded that treatment of grain with the fungicide Matador could result in a marked stimulation of the production of NIV by F. culmorum. As the incidence of Fusarium headblight did not correlate with the amount of mycotoxins found, the need for mycotoxin analyses combined with bioassays such as the MTT-cell culture assay is recommended for a meaningful assessment of the quality of grain treated in such a manner.

Topics: Animals; Biological Assay; Cells, Cultured; Fungicides, Industrial; Fusarium; Gas Chromatography-Mass Spectrometry; Mycotoxins; Nitriles; Pyrethrins; Swine; Triazoles; Trichothecenes; Triticum