cycloclavine has been researched along with cyclopropane* in 1 studies
1 other study(ies) available for cycloclavine and cyclopropane
| Article | Year |
|---|---|
Discovery and reconstitution of the cycloclavine biosynthetic pathway--enzymatic formation of a cyclopropyl group.
The ergot alkaloids, a class of fungal-derived natural products with important biological activities, are derived from a common intermediate, chanoclavine-I, which is elaborated into a set of diverse structures. Herein we report the discovery of the biosynthetic pathway of cycloclavine, a complex ergot alkaloid containing a cyclopropyl moiety. We used a yeast-based expression platform along with in vitro biochemical experiments to identify the enzyme that catalyzes a rearrangement of the chanoclavine-I intermediate to form a cyclopropyl moiety. The resulting compound, cycloclavine, was produced in yeast at titers of >500 mg L(-1) , thus demonstrating the feasibility of the heterologous expression of these complex alkaloids. Topics: Aspergillus fumigatus; Cyclopropanes; Enzymes; Ergot Alkaloids; Fungal Proteins; Indole Alkaloids; Multigene Family; Saccharomyces cerevisiae | 2015 |