cyclo(prolylprolyl) and 4-hydroxyphenylacetic-acid

Low molecular weight compounds were isolated by high-performance liquid chromatography from the maggot or haemolymph extracts of Lucilia sericata (Meigen) (Diptera: Calliphoridae). Using gas chromatography-mass spectrometry analysis, three compounds were obtained: p-hydroxybenzoic acid (molecular weight 138 Da), p-hydroxyphenylacetic acid (molecular weight 152 Da) and octahydro-dipyrrolo[1,2-a;1',2'-d] pyrazine-5,10-dione (molecular weight 194 Da), also known as the cyclic dimer of proline (or proline diketopiperazine or cyclo[Pro,Pro]). All three molecules revealed antibacterial activity when tested against Micrococcus luteus and/or Pseudomonas aeruginosa, and the effect was even more pronounced when these molecules were tested in combination and caused lysis of these bacteria.

Topics: Animals; Anti-Bacterial Agents; Chromatography, High Pressure Liquid; Diptera; Hemolymph; Hydroxybenzoates; Larva; Microbial Sensitivity Tests; Micrococcus luteus; Molecular Weight; Peptides, Cyclic; Phenylacetates; Pseudomonas aeruginosa; Stem Cells; Time Factors