cyclin-d1 has been researched along with tylophorine* in 1 studies
1 other study(ies) available for cyclin-d1 and tylophorine
| Article | Year |
|---|---|
Tylophorine reduces protein biosynthesis and rapidly decreases cyclin D1, inhibiting vascular smooth muscle cell proliferation in vitro and in organ culture.
Tylophorine (TYL) is an alkaloid with antiproliferative action in cancer cells. Vascular smooth muscle cell (VSMC) proliferation and neointima formation contribute to restenosis after percutaneous coronary interventions.. Our goal was to examine the potential of TYL to inhibit VSMC proliferation and migration, and to dissect underlying signaling pathways.. TYL was administered to platelet-derived growth factor (PDGF-BB)-stimulated, serum-stimulated, quiescent and unsynchronized VSMC of rat and human origin. BrdU incorporation and resazurin conversion were used to assess cell proliferation. Cell cycle progression was analyzed by flow cytometry of propidium iodide-stained nuclei. Expression profiles of proteins and mRNAs were determined using western blot analysis and RT-qPCR. The Click-iT OPP Alexa Fluor 488 assay was used to monitor protein biosynthesis.. This unique activity profile renders TYL an interesting lead for the treatment of vasculo-proliferative disorders, such as restenosis. Topics: Alkaloids; Animals; Becaplermin; Cell Cycle; Cell Proliferation; Cyclin D1; Down-Regulation; Gene Expression Regulation; Humans; Indolizines; Myocytes, Smooth Muscle; Organ Culture Techniques; Phenanthrenes; Protein Biosynthesis; Rats; Rats, Sprague-Dawley; Signal Transduction; Umbilical Veins | 2019 |