cyclin-d1 and isoprene

cyclin-d1 has been researched along with isoprene* in 2 studies

Other Studies

2 other study(ies) available for cyclin-d1 and isoprene

ArticleYear
Cigarette smoke extract and isoprene resulted in the induction of apoptosis and autophagy in human placenta choriocarcinoma JEG-3 cells.
    Environmental toxicology, 2018, Volume: 33, Issue:2

    In this study, the effects of cigarette smoke (CS) on the induction of apoptosis via reactive oxygen species (ROS) production and endoplasmic reticulum stress (ER stress) of JEG-3 human choriocarcinoma cells were examined to confirm the relationship between CS and placenta development. Upon TUNEL assay, CS extract (3R4F; 0.3 and 2.1 μM) increased JEG-3 apoptosis. Western blot assay revealed that the protein expressions of p53, Bax, and CCAAT-enhancer-binding protein homologous protein (CHOP) increased, while the levels of Bcl-2 were reduced following CS extract treatment. Moreover, 2',7'-dichlorofluorescein diacetate (DCFH-DA) assay revealed increased ROS production. Upon 3-(4-5-dimethylthiazol-2-yl)-2.5-dyhphenyltetrazolium bromide (MTT) assay, isoprene (IP), one of ingredients of CS, deceased JEG-3 cell viability (10

    Topics: Apoptosis; Autophagy; bcl-2-Associated X Protein; Butadienes; Cell Line, Tumor; Choriocarcinoma; Cyclin D1; Cyclin E; Endoplasmic Reticulum Stress; Female; Hemiterpenes; Humans; Kelch-Like ECH-Associated Protein 1; NF-E2-Related Factor 2; Nicotiana; Oncogene Proteins; Pentanes; Pregnancy; Reactive Oxygen Species; Sequestosome-1 Protein; Smoke; Transcription Factor CHOP; Tumor Suppressor Protein p53; Uterine Neoplasms

2018
Three components of cigarette smoke altered the growth and apoptosis of metastatic colon cancer cells via inducing the synthesis of reactive oxygen species and endoplasmic reticulum stress.
    Environmental toxicology and pharmacology, 2016, Volume: 45

    Cigarette smoke (CS) is a well-known risk factor for carcinogenesis and has been found to be related to the occurrence and development of colon cancer. In this study, the effect of formaldehyde (FA), benzene (Bz), and isoprene (IP), which are included in main components of CS, on cell viability and apoptosis of SW620 colorectal cancer cells was examined to identify the connection between CS components and colon cancer. In cell viability assay, FA, Bz, and IP decreased cell viability of SW620 cells in a dose dependent manner. In Western blot assay, the protein expression of cell cycle related genes, cyclin D1 & E1, was decreased by FA, Bz, and IP, which corresponded to their inhibitory effect on cell viability. In addition, FA, Bz, and IP increased the protein expression of pro-apoptotic genes, C/EBP homologous protein (CHOP) and Bax, and reduced the protein expression of anti-apoptotic gene, Bcl-2. In reactive oxygen species (ROS) assay using dichlorofluorescin diacetate (DCFH-DA), FA, Bz, and IP increased the ROS production in SW620 cells. In the measurement of apoptotic cells, the numbers of apoptotic cells were increased by the treatment of FA, Bz, and IP. As CHOP is an endoplasmic reticulum (ER)-stress related apoptosis marker of which production is induced by ROS, it was considered that these CS components induce apoptosis of SW620 cells by increasing ROS synthesis and ER-stress. Taken together, these results showed that CS components, i.e., FA, Bz, and IP, inhibited the cell viability of SW620 cells by down-regulating the protein expression of cyclin D1 & E1 and induced apoptosis of SW620 cells by increasing ROS production and simultaneously activating ER-stress.

    Topics: Apoptosis; bcl-2-Associated X Protein; Benzene; Butadienes; Cell Line, Tumor; Cell Proliferation; Colonic Neoplasms; Cyclin D1; Cyclin E; Endoplasmic Reticulum Stress; Formaldehyde; Hemiterpenes; Humans; Nicotiana; Oncogene Proteins; Pentanes; Proto-Oncogene Proteins c-bcl-2; Reactive Oxygen Species; Smoke; Transcription Factor CHOP

2016
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