cyclic-gmp and ethylene

cGMP promotes ethylene production and enhances the perception of ethylene. Endogenous ethylene or cGMP accumulation maintains ion homeostasis to enhancing salt resistance. etr1 - 3 is insensitive to cGMP under salt stress.. In the present study, we presented a signaling network involving ethylene and cGMP in salt resistance pathway of Arabidopsis roots. Results showed that the ethylene-insensitive mutant etr1-3 was more sensitive to salt stress than the wild type (WT). etr1-3 displayed a greater electrolyte leakage, thiobarbituric acid reactive substances and Na(+)/K(+) ratio, but a lower plasma membrane (PM) H(+)-ATPase activity compared to WT under the different NaCl contents. Application of 1-aminocyclopropane-1-carboxylic acid (ACC, an ethylene precursor) or 8-Br-cGMP (the cGMP analog) alleviated NaCl-induced injury by maintaining a lower Na(+)/K(+) ratio and increasing PM H(+)-ATPase activity in WT, but not in etr1-3. Roots treated with 8-Br-cGMP could promote ethylene production and enhance the expression of ACC synthase gene in WT. In addition, the 8-Br-cGMP action in NaCl stress was inhibited by aminooxyacetic acid (an inhibitor of ethylene biosynthesis), but 6-Anilino-5,8-quinolinedione (Ly83583, a guanylate cyclase inhibitor) could not affect ACC action in WT. These results suggest that ethylene functions as a downstream signal of cGMP that stimulates the PM H(+)-ATPase activity, which finally results in regulating ion homeostasis in Arabidopsis tolerance to salt. Moreover, cGMP enhanced the perception of ethylene in Arabidopsis under salt stress, which reversed the salt-induced increase of ETR1 and increased ERF1 at the transcript levels in WT. In a word, cGMP modulates salt resistance pathway of ethylene through regulating biosynthesis and perception of ethylene in Arabidopsis roots.

Topics: Amino Acids, Cyclic; Arabidopsis; Cyclic GMP; Ethylenes; Ions; Plant Roots; Sodium Chloride

MicroRNAs (miRNAs) are small noncoding RNAs which post-transcriptionally regulate gene expression by directing mRNA cleavage or translational inhibition. miRNAs play multiple roles in the growth, development and stress responses in plants. However, little is known of the wounding-responsive miRNAs and their regulation. Here, we investigated the expression patterns of microR828 (miR828) on wounding in sweet potato (Ipomoea batatas cv Tainung 57). The expression of miR828 was only detected in leaves, and was induced by wounding rather than by ethylene, hydrogen peroxide (H2O2), methyl jasmonate or nitric oxide (NO). Moreover, cyclic guanosine monophosphate (cGMP) was necessary for miR828 accumulation in leaves on wounding. Two miR828 target candidates, named IbMYB and IbTLD, were obtained by cDNA cloning, and their mRNA cleavage caused by miR828 was confirmed by cleavage site mapping, agro-infiltration and transgenics studies. The reduction in IbMYB and IbTLD expression coincided with the induction of miR828, demonstrating that IbMYB and IbTLD might be miR828 targets. Furthermore, transgenic sweet potato overexpressing miR828 precursor affected lignin and H2O2 contents. These results showed that cGMP could regulate wounding-responsive miR828, which repressed the expression of IbMYB and IbTLD. Subsequently, lignin and H2O2 were accumulated to participate in defense mechanisms.

Topics: Acetates; Agrobacterium; Antioxidants; Base Sequence; Calcium; Cyclic ADP-Ribose; Cyclic GMP; Cyclopentanes; Ethylenes; Gene Expression Profiling; Gene Expression Regulation, Plant; Hydrogen Peroxide; Ipomoea batatas; Lignin; MicroRNAs; Molecular Sequence Data; Niacinamide; Nitric Oxide; Okadaic Acid; Oxylipins; Phosphoprotein Phosphatases; Plant Proteins; Plants, Genetically Modified; Propanols; Protein Kinases; RNA, Messenger; Staurosporine; Stress, Mechanical