cyanogen bromide has been researched along with sepharose in 166 studies
| Timeframe | Studies, this research(%) | All Research% |
|---|---|---|
| pre-1990 | 119 (71.69) | 18.7374 |
| 1990's | 26 (15.66) | 18.2507 |
| 2000's | 11 (6.63) | 29.6817 |
| 2010's | 9 (5.42) | 24.3611 |
| 2020's | 1 (0.60) | 2.80 |
| Authors | Studies |
|---|---|
| Kosugi, Y; Suzuki, H | 1 |
| Goheer, MA; Gould, BJ; Parke, DV | 1 |
| Goheer, MA; Gould, BJ | 1 |
| Coombe, RG; George, AM | 1 |
| Galka, M; Ostrowski, W; Torchilin, VP | 1 |
| Bevilacqua, R; Chaiken, IM; Veronese, FM | 1 |
| Deelder, AM; Streefkerk, JG | 1 |
| Dankert, J; Goudeau, A; Houwen, B | 1 |
| Ichiki, AT; Lange, RD; Quirin, YP; Shelton, RN | 1 |
| Kors, N; Streefkerk, JG; van der Ploeg, M | 1 |
| Knauf, S; Urbach, GI | 1 |
| Chance, B; Pye, EK | 1 |
| Miura, Y; Miyamoto, K; Raze, JM; Urabe, H | 1 |
| Parikh, I; Slovin, SF | 1 |
| Zola, H | 1 |
| Chantler, S; Raybould, TJ | 1 |
| Levi, AS | 2 |
| Capel, PJ | 1 |
| Light, A; Sinha, NK | 1 |
| Borriss, E; Koch, G | 1 |
| Eriksson, K; Froehner, SC | 1 |
| Dean, DG; Potuzak, H | 1 |
| Soria, C; Soria, J | 1 |
| Daus, H; Kümel, G; Mauch, H | 1 |
| Borecký, L; Fuchsberger, N; Hajnicka, V | 1 |
| Björk, I; Nordenman, B | 1 |
| Finlay, TH; Hodgins, LT; Johnson, AJ; Levy, M; Troll, V | 1 |
| Berman, AE; Gornaeva, NP; Mazurov, VI | 1 |
| Kohn, J; Wilchek, M | 4 |
| Chan, WW | 1 |
| Cheng, WC; Ng, J; Vedros, N; Webb, E | 1 |
| Oka, T; Topper, YJ; Vonderhaar, BK; Wilchek, M | 1 |
| Jacobs, NF; Scales, RW; Skaggs, R | 1 |
| Miyagawa, A; Okuyama, T | 1 |
| Hane, Y; Miura, Y; Miyamoto, K; Nagata, C; Urabe, H | 1 |
| Blombäck, B; York, LL | 1 |
| Van Eijk, HG; Van Noort, WL | 1 |
| Werber, MM | 1 |
| Oka, T; Topper, YJ; Wilchek, M | 1 |
| Flemming, C; Kleine, R; Spangenberg, P | 1 |
| Bailon, P; Nishikawa, AH | 2 |
| Arndt-Jovin, DJ; Bähr, W; Frischauf, AM; Jovin, TM; Marquardt, M | 1 |
| Deelder, AM; Ploem, JS; Snoijink, JJ | 1 |
| Bradlow, HL; Rosner, W | 1 |
| Borchardt, RT; Cheng, CF; Thakker, DR | 1 |
| Fey, H; Wetzstein, HP | 1 |
| Hofstee, BH | 1 |
| Hanson, H; Koelsch, R; Lasch, J; Marquardt, I | 1 |
| Hanoune, J; Lacombe, ML; Strosberg, AD; Vanquelin, G | 1 |
| Holmberg, L; Jeppsson, JO; Nilsson, IM; Stenflo, J | 1 |
| Bolander, FF; Fellows, RE | 1 |
| Gribnau, TC; Stumm, C; Tesser, GI | 1 |
| Eckstein, F; Goumet, M; Wetzel, R | 1 |
| Baess, D; Pommerening, K; Ruckpaul, K | 1 |
| Fell, DA; White, CJ | 1 |
| Alonso, A; Kinzel, V; Kübler, D | 1 |
| Iwig, M; Koelsch, R; Lasch, J | 1 |
| Carter, WA; Davey, MW; Sulkowski, E | 1 |
| Komori, F; Maeno, M; Naganuma, K; Nakano, K; Ogoshi, T; Otsuka, K; Suzuki, K; Suzuki, N; Taguchi, M; Tamura, Y | 1 |
| Desai, MA; Lyddiatt, A | 1 |
| Dellacherie, E; Hubert, P; Regnault, V; Rivat, C; Ubrich, N | 1 |
| Mallia, AK; Nevens, JR; Smith, PK; Wendt, MW | 1 |
| Novak, RF; Primiano, T | 1 |
| Gilissen, RA; Meerman, JH; Mulder, GJ | 1 |
| Griffith, OH; Keana, JF; Shashidhar, MS; Volwerk, JJ | 1 |
| Collen, D; De Cock, F; Lijnen, HR; Nelles, L; Van Hoef, B | 1 |
| Ohashi, A; Yasuhara, T | 1 |
| Fischer, M; Halbmayer, M; Hopmeier, P; Marx, G | 1 |
| Braun, RW; Kümel, G | 1 |
| Batt, AM; Decolin, M; Dellacherie, E; Ibrahim, M; Siest, G | 1 |
| Iakubovskaia, RI; Korostelova, MD; Nemtsova, ER | 1 |
| Korpela, T; Kurkijärvi, K | 2 |
| Berndt, P; Miasoedova, KN | 1 |
| Fellhofer, G; Pittner, F; Rapatz, E; Travnicek, A | 1 |
| Pfeiffer, NE; Schuster, SM; Wylie, DE | 1 |
| Han, GY; Han, PF; Johnson, J; McBay, HC; Wang, YH | 1 |
| Ballesteros, A; Guisan, JM; Melo, FV; Serrano, J | 1 |
| Diaz-Mauriño, T; Tercero, JC | 1 |
| Ito, Y; Matsumoto, I; Seno, N; Yamasaki, Y | 1 |
| Born, J; Hoppe, P; Janeczek, J; Tiedemann, H | 1 |
| Dalzoppo, D; Fontana, A; Pastorino, AM | 1 |
| Bacigalupo, MA; Carrea, G | 1 |
| Chibata, I; Matuo, Y; Tosa, T | 1 |
| Harper, AA; Olsnes, S; Pappenheimer, AM | 1 |
| Ayad, SR; Shimmin, ER | 1 |
| Andersson, LO; Wichman, A | 1 |
| Shaltiel, S | 1 |
| Kristiansen, T | 1 |
| Marshall, JS; Pensky, J | 1 |
| Larsson, PO; Mosbach, K | 1 |
| Bauer, EA; Eisen, AZ; Jeffrey, JJ; Stricklin, GP | 1 |
| Cuatercasas, P; March, S; Parikh, I | 1 |
| Arnon, R; Ben-Yoseph, Y; Geiger, B | 1 |
| Jakubke, HD; Lange, L | 1 |
| Brosseau, JD; Shobe, CR | 1 |
| Barker, R; Chiang, CK; Hill, RL; Trayer, IP | 1 |
| Vutz, R; Woenckhaus, C | 1 |
| Scherer, G; Weber, H; Weis, W | 1 |
| Irie, M; Nishimura, T; Ogi, K | 1 |
| Chino, TH; David, GS; Reisfeld, RA | 1 |
| Fukui, S; Ikeda, SI; Sumi, Y | 1 |
| Igloi, GL | 1 |
| Ji, XS; Li, HX; Liu, SH; Yuan, ZY | 1 |
| Kuzovleva, OB; Shakhanina, KL | 1 |
| Aplin, JD; Hall, LD | 1 |
| Langone, JJ; Sukumar, S; Terata, N; Zbar, B | 1 |
| Halperin, G; Shaltiel, S; Tauber-Finkelstein, M | 1 |
| Charbonneau, H; Cormier, MJ; Hart, RC; Hice, R | 1 |
| Barnes, JA; Kennedy, JF | 1 |
| Ferragut, JA; Iborra, JL; Lozano, JA | 1 |
| Andrews, AT | 2 |
| Bar-Tana, J; Rosen, G | 1 |
| Winkelman, L | 1 |
| Johnson, KE | 1 |
| Gibbons, JJ; Pohl, DA; Roodman, ST; Tsai, CC | 1 |
| McCracken, S; Meighen, E | 1 |
| Slavík, K; Slavíková, V | 1 |
| Zakrevskiĭ, VI | 1 |
| Hseu, TH; Lan, SL; Yang, MD | 1 |
| Fukui, S; Toraya, T | 1 |
| Harrison, JH; Jurgensen, SR; Mahler, JC; Wood, DC | 1 |
| Brassfield, AL | 1 |
| Kuroda, M; Yoshihara, Y | 1 |
| Kent, UM | 1 |
| Gupta, AK; Gupta, RK; Hazra, DK; Khanna, P; Khanna-Hazra, P; Lahiri, VL; Painuly, NK; Pathak, M; Sharma, SK; Srivastava, RK | 1 |
| Hendriks, DF; Scharpé, SL; Wang, W | 1 |
| Béress, R; Euler, HH; Schroeder, JO; Zeuner, RA | 1 |
| Chiancone, E; Gattoni, M | 1 |
| Bubb, MO; Conradie, JD; Stubbings, D | 1 |
| Beer, DJ; Jack, GW; Randles, SC; Yates, AM | 1 |
| Rivat, C; Ubrich, N | 1 |
| Beer, DJ; Jack, GW | 1 |
| Azimzadeh, A; Cinqualbre, J; Lucchiari, N; Regnault, V; Wolf, P | 1 |
| Höltje, JV; Ursinus, A; von Rechenberg, M | 1 |
| Fujita, K | 1 |
| Gustavsson, PE; Larsson, PO | 1 |
| Abo, S; Alafaci, A; Ciccotosto, S; von Itzstein, M | 1 |
| Gorny, MK; Hochleitner, EO; Tomer, KB; Zolla-Pazner, S | 1 |
| Sundaram, PV | 1 |
| Befani, O; Federico, R; Mateescu, MA; Mondovì, B; Mulhbacher, J | 1 |
| He, RQ; Wu, C; Wu, XQ | 1 |
| Dai, L; Huang, X; Liang, P; Ren, G; Ye, H | 1 |
| Beldarraín, A; Expósito, N; Gómez, L; Hernández, R; Martínez, E; Páez, R; Plana, L; Valdés, J | 1 |
| Ghosh, S; Gupta, SC; Rawat, P; Singh, BP | 1 |
| Frey, SK; Gontarev, S; Kvach, M; Schweigert, FJ; Shmanai, V | 1 |
| Kadonaga, JT; Kerrigan, LA | 2 |
| Scouten, WH | 1 |
| Springer, TA | 1 |
| Homaei, AA; Sajedi, RH; Sariri, R; Seyfzadeh, S; Stevanato, R | 1 |
| Archakov, AI; Kopylov, AT; Lisitsa, AV; Zgoda, VG | 1 |
| Højrup, P; Zhang, X | 1 |
| Kavran, JM; Leahy, DJ | 1 |
| Angeletti, RH; Hawke, DH; Nika, H | 1 |
| de Sant' Ana, HB; dos Santos, JC; Fernandez-Lafuente, R; Garcia-Galan, C; Gonçalves, LR; Rodrigues, RC | 1 |
| Homaei, A | 1 |
| Dos Santos, JC; Fernandez-Lafuente, R; Freire, DM; Manoel, EA; Rueda, N | 1 |
| Fernández-Lorente, G; Guisan, JM; Moreno-Pérez, S; Orrego, AH; Rocha-Martin, J; Trobo-Maseda, L | 1 |
| Chojnacka, A; Drozd, R; Gładkowski, W; Leśniarek, A; Szymańska, M | 1 |
2 review(s) available for cyanogen bromide and sepharose
| Article | Year |
|---|---|
Affinity chromatography on columns containing nucleic acids.
Topics: Adsorption; Carrier Proteins; Cellulose; Chromatography, Affinity; Cyanogen Bromide; DNA; Epoxy Compounds; Phosphates; Polysaccharides; Sepharose; Ultraviolet Rays | 1978 |
[Immunosorbents and their application to immunology of microorganisms].
Topics: Acrylamides; Antigen-Antibody Complex; Antigen-Antibody Reactions; Cellulose; Chemical Phenomena; Chemistry; Chromatography, Affinity; Cyanogen Bromide; Enterotoxins; Fluorescent Antibody Technique; Immunosorbent Techniques; Immunosorbents; Microbiological Techniques; Polymers; Sepharose; Solubility; Staphylococcus aureus | 1980 |
164 other study(ies) available for cyanogen bromide and sepharose
| Article | Year |
|---|---|
Affinity chromatography of lipase with hydrophobic ligands coupled to cyanogen bromide-activated agarose.
Topics: Amines; Chromatography, Affinity; Cyanogen Bromide; Hydrogen-Ion Concentration; Ligands; Lipase; Pseudomonas; Sepharose; Structure-Activity Relationship | 1976 |
Preparation of immobilized baker's-yeast glucose 6-phosphate dehydrogenase attached to modified sepharose and sephadex and a comparison of the properties of these preparations with those of the soluble enzyme.
Topics: Buffers; Cyanogen Bromide; Glucosephosphate Dehydrogenase; Hydrogen-Ion Concentration; Saccharomyces cerevisiae; Sepharose; Solubility; Temperature | 1976 |
Kinetic mechanism from steady-state kinetics of the reaction catalysed by baker's-yeast glucose 6-phosphate dehydrogenase in solution and covalently attached to sepharose.
Topics: Cyanogen Bromide; Glucosephosphate Dehydrogenase; Kinetics; NADP; Saccharomyces cerevisiae; Sepharose | 1976 |
An alternative coupling procedure for preparing activated sepharose for affinity chromatography of penicillinase.
Topics: Chemical Phenomena; Chemistry; Chromatography, Affinity; Chymotrypsin; Cyanogen Bromide; Enzymes, Immobilized; Ethylenediamines; Hydrogen-Ion Concentration; Methicillin; Penicillinase; Sepharose; Sulfones | 1976 |
Comparative studies on immobilization of human prostatic acid phosphatase.
Topics: Acid Phosphatase; Concanavalin A; Cyanogen Bromide; Enzymes, Immobilized; Ethylenediamines; Humans; Hydrogen-Ion Concentration; Kinetics; Male; Prostate; Sepharose | 1977 |
Drug-protein interactions: evaluation of the binding of antipsychotic drugs to glutamate dehydrogenase by quantitative affinity chromatography.
Topics: Antipsychotic Agents; Butyrophenones; Chlorpromazine; Chromatography, Affinity; Cyanogen Bromide; Glutamate Dehydrogenase; Kinetics; Perphenazine; Protein Binding; Sepharose | 1979 |
Serodiagnostic application of immunohistoperoxidase reactions on antigen-coupled agarose beads.
Topics: Aminobutyrates; Animals; Antibodies; Antigens; Cattle; Cyanogen Bromide; gamma-Globulins; Goats; Hemagglutination Tests; Humans; Immune Sera; Peroxidases; Rabbits; Schistosoma mansoni; Sepharose; Serologic Tests; Spectrophotometry; Staining and Labeling; Swine | 1975 |
Isolation of hepatitis B surface antigen (HBsAg) by affinity chromatography on antibody-coated immunoadsorbents.
Topics: ABO Blood-Group System; Adsorption; Animals; Blood Proteins; Chemical Fractionation; Chromatography, Affinity; Chromatography, DEAE-Cellulose; Cyanogen Bromide; Erythrocytes; gamma-Globulins; Guinea Pigs; Hemagglutination Tests; Hepatitis B; Hepatitis B Antibodies; Hepatitis B Antigens; Immunodiffusion; Methods; Sepharose; Tannins | 1975 |
Sepharose-bound erythropoientin. Studies with anti-erythropoietin.
Topics: Animals; Antibodies; Binding Sites, Antibody; Chromatography, Affinity; Cyanogen Bromide; Epitopes; Erythropoietin; Humans; Immunization; Immunologic Techniques; Neutralization Tests; Polycythemia Vera; Rabbits; Sepharose | 1975 |
Some aspects of immunohistoperoxidase reactions in the agarose bead model system.
Topics: Adhesiveness; Aminobiphenyl Compounds; Animals; Antibodies; Antibody Specificity; Antigens; Cattle; Cyanogen Bromide; Fluorescent Antibody Technique; gamma-Globulins; Hydrochloric Acid; Immune Sera; Ovalbumin; Peroxidases; Rabbits; Sepharose; Spectrophotometry; Staining and Labeling | 1975 |
Purification of human ovarian tumor-associated antigen and demonstration of circulating tumor antigen in patients with advanced ovarian malignancy.
Topics: Animals; Antigens, Neoplasm; Chromatography, Affinity; Concanavalin A; Cyanogen Bromide; Female; gamma-Globulins; Humans; Immune Sera; Immunodiffusion; Immunoelectrophoresis; Mannose; Ovarian Neoplasms; Ovary; Rabbits; Sepharose | 1977 |
Investigation of the physical properties of immobilized enzymes.
Topics: Circular Dichroism; Cyanogen Bromide; Electron Spin Resonance Spectroscopy; Enzymes, Immobilized; Evaluation Studies as Topic; Gels; Magnetic Resonance Spectroscopy; Methods; Optical Rotatory Dispersion; Protein Binding; Protein Conformation; Sepharose; Solubility; Spectrometry, Fluorescence; Spectrophotometry | 1976 |
Immobilization of urea cycle enzymes. I. Characterization of immobilized carbamoylphosphate synthetase and ornithine carbamoyltransferase.
Topics: Animals; Anura; Carbamoyl-Phosphate Synthase (Ammonia); Cyanogen Bromide; Enzymes, Immobilized; In Vitro Techniques; Liver; Ornithine Carbamoyltransferase; Phosphotransferases; Rana catesbeiana; Sepharose | 1977 |
An introduction to affinity chromatography.
Topics: Buffers; Chromatography, Affinity; Cyanogen Bromide; Enzymes; Galactosidases; Indicators and Reagents; L-Lactate Dehydrogenase; Periodic Acid; Quinones; Sepharose; Triazines | 1977 |
Immunoabsorbents prepared from cell membrane antigens: efficiency of incorporation and shedding of protein during use.
Topics: Antigen-Antibody Complex; Antigens; Antilymphocyte Serum; Cell Membrane; Cyanogen Bromide; Epoxy Compounds; Glutaral; Humans; Immunosorbents; Membrane Proteins; Periodic Acid; Sepharose | 1978 |
Comparison of activation and blocking procedures in the use of defined antigen substrate spheres (DASS) for quantitative and visual assessment of conjugate activity.
Topics: Antigens; Cyanogen Bromide; Fluorescent Antibody Technique; Humans; Immunoglobulin G; Methods; Periodic Acid; Protein Binding; Quinones; Sepharose | 1979 |
Properties of water-insoluble matrix-bound lactate dehydrogenase.
Topics: Animals; Chickens; Complement Fixation Tests; Cyanogen Bromide; Dogfish; Hydrogen-Ion Concentration; Isoenzymes; Kinetics; L-Lactate Dehydrogenase; Muscles; Myocardium; Protein Binding; Sepharose; Solubility; Water | 1975 |
The defined antigen substrate spheres (DASS) system and some of its applications.
Topics: Antibodies; Antigens; Complement System Proteins; Cyanogen Bromide; Ethanolamines; Fluorescent Antibody Technique; Hydrogen-Ion Concentration; Immunoglobulins; Microscopy, Electron; Molecular Weight; Polymers; Polysaccharides; Sepharose; Solubility; Solvents | 1975 |
Refolding of reduced, denatured trypsinogen and trypsin immobilized on Agarose beads.
Topics: Animals; Binding Sites; Cattle; Cyanogen Bromide; Disulfides; Hydrogen-Ion Concentration; Kinetics; Protein Binding; Protein Conformation; Protein Denaturation; Sepharose; Solubility; Temperature; Trypsin; Trypsinogen | 1975 |
Isolation of poliovirus-neutralizing antibodies by affinity chromatography.
Topics: Antibodies, Viral; Antigen-Antibody Reactions; Chromatography, Affinity; Cyanogen Bromide; Humans; Hydrogen-Ion Concentration; Neutralization Tests; Poliovirus; Sepharose | 1975 |
Properties of the glycoprotein laccase immobilized by two methods.
Topics: Catechol Oxidase; Concanavalin A; Cyanogen Bromide; Enzyme Activation; Guaiacol; Hydrogen-Ion Concentration; Kinetics; Neurospora crassa; Oxygen Consumption; Protein Binding; Sepharose | 1975 |
Determination of fibrinogen degradation products by immuno-enzymological assay.
Topics: Animals; Antigen-Antibody Complex; Cattle; Chemical Precipitation; Cyanogen Bromide; Fibrin Fibrinogen Degradation Products; Fibrinogen; Humans; Immunoenzyme Techniques; Immunoglobulin G; Immunosorbents; Rabbits; Sepharose | 1978 |
Improved method for the cyanogen bromide activation of agarose beads.
Topics: Animals; Antigens; Cyanogen Bromide; Immunoglobulin G; Immunosorbent Techniques; Polysaccharides; Protein Binding; Rabbits; Sepharose; Swine | 1979 |
Purification of mouse interferon on specifically purified immunoadsorbent.
Topics: Animals; Antigen-Antibody Complex; Cyanogen Bromide; Immunosorbents; Interferons; L Cells; Mice; Sepharose | 1977 |
Purification of thrombin by affinity chromatography on immobilized heparin.
Topics: Animals; Cattle; Chromatography, Affinity; Cyanogen Bromide; Electrophoresis, Disc; Heparin; Prothrombin; Sepharose; Thrombin | 1977 |
New methods for the preparation of biospecific adsorbents and immobilized enzymes utilizing trichloro-s-triazine.
Topics: Adsorption; Aminocaproates; Chemical Phenomena; Chemistry; Chromatography, Affinity; Cyanogen Bromide; Enzymes, Immobilized; Humans; Ligands; Sepharose; Serum Albumin, Bovine; Solubility; Triazines | 1978 |
[Biospecific chromatography of poly(A)-containing RNA on poly(U)-Sepharose].
Topics: Chemical Phenomena; Chemistry; Chromatography, Gel; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Ethanolamines; Poly A; Poly U; RNA; Sepharose | 1978 |
A colorimetric method for monitoring activation of Sepharose by cyanogen bromide.
Topics: Colorimetry; Cyanates; Cyanogen Bromide; Kinetics; Polysaccharides; Sepharose | 1978 |
Immobilized subunits.
Topics: Cyanogen Bromide; Enzymes, Immobilized; Evaluation Studies as Topic; Fructose-Bisphosphate Aldolase; Macromolecular Substances; Methods; Muscles; Protein Binding; Saccharomyces cerevisiae; Sepharose; Solubility; Transaldolase | 1976 |
Fractionation and characterization of surface antigens from group A Neisseria meningitidis.
Topics: Amino Acids; Animals; Antigens, Bacterial; Bacteriological Techniques; Blood Bactericidal Activity; Butylamines; Carbohydrates; Chemical Fractionation; Cyanogen Bromide; Dimethylformamide; DNA; Electrophoresis, Disc; Immune Sera; Immunization; Immunization, Secondary; Immunologic Memory; Mice; Mice, Inbred Strains; Neisseria meningitidis; Nitrogen; Precipitin Tests; Rabbits; RNA; Sepharose; Skin Tests; Spectrophotometry | 1975 |
Characterization of super-active insulin, prolactin and placental lactogen.
Topics: Animals; Cattle; Cyanogen Bromide; Diaphragm; Female; Humans; Insulin; Lactose Synthase; Mammary Glands, Animal; Mice; Mice, Obese; Placental Lactogen; Pregnancy; Prolactin; Sepharose | 1975 |
Use of immunoglobulin coupled to agarose beads for examining the specificity of conjugates.
Topics: Antibody Specificity; Antigen-Antibody Reactions; Cross Reactions; Cyanogen Bromide; Evaluation Studies as Topic; Fluorescent Antibody Technique; Immunoelectrophoresis; Immunoglobulin G; Immunoglobulin Light Chains; Immunoglobulins; Microscopy, Fluorescence; Sepharose; Serum Albumin, Bovine | 1975 |
An improved estimation method for amino alkyl ligand immobilized on matrix beads.
Topics: Amines; Cyanogen Bromide; Drug Stability; Kinetics; Ligands; Macromolecular Substances; Methods; Polysaccharides; Sepharose; Serum Albumin, Bovine; Spectrophotometry; Spectrophotometry, Ultraviolet; Trinitrobenzenesulfonic Acid; Tyrosine | 1977 |
Immobilization of urea cycle enzymes. II. Characterization of immobilized argininosuccinate synthetase.
Topics: Animals; Argininosuccinate Synthase; Cattle; Cyanogen Bromide; Enzymes, Immobilized; In Vitro Techniques; Ligases; Liver; Sepharose | 1977 |
Interaction of fragments of fibrinogen with insolubilized fibrin monomers (activated fibrinogen).
Topics: Chemical Phenomena; Chemistry; Cyanogen Bromide; Fibrin; Fibrinogen; Fibrinopeptide A; Protein Binding; Sepharose; Thrombin | 1976 |
Isolation of rat transferrin using CNBr-activated sepharose 4B.
Topics: Amino Acids; Animals; Chromatography, Affinity; Cyanogen Bromide; Isoelectric Focusing; Rats; Sepharose; Sialic Acids; Transferrin | 1976 |
Nucleophilicity of isourea linkages in substituted agaroses. A direct method for the determination of alkylamino groups coupled to CNBr-activated agarose.
Topics: Amines; Binding Sites; Chemical Phenomena; Chemistry; Chromatography, Affinity; Cyanogen Bromide; Kinetics; Methods; Polysaccharides; Sepharose; Urea | 1976 |
Structure of a soluble super-active insulin is revealed by the nature of the complex between cyanogen-bromide-activated sepharose and amines.
Topics: Amines; Animals; Cyanogen Bromide; Glucose; Guanidines; Insulin; Mice; Mice, Obese; Polysaccharides; Sepharose; Serum Albumin, Bovine; Structure-Activity Relationship | 1975 |
[Purification of an anionic trypsin from the gastric juice of the crayfish Cambarus affinis say by means of affinity chromatography].
Topics: Animals; Aprotinin; Astacoidea; Benzamidines; Benzidines; Chromatography, Affinity; Cyanogen Bromide; Gastric Juice; Glass; Methods; Sepharose; Trypsin | 1975 |
Affinity purification methods. Nonspecific adsorption of proteins due to ionic groups in cyanogen bromide treated agarose.
Topics: Adipates; Anhydrides; Binding Sites; Chemical Phenomena; Chemistry; Chromatography, Affinity; Cyanogen Bromide; Diamines; Escherichia coli; Galactosidases; Hydrazines; Phenylacetates; Polysaccharides; Protein Binding; Sepharose; Serum Albumin, Bovine; Succinates; Tosyl Compounds | 1975 |
Covalent attachment of DNA to agarose. Improved synthesis and use in affinity chromatography.
Topics: Animals; Binding Sites; Cattle; Chromatography, Affinity; Cyanogen Bromide; DNA; DNA Nucleotidyltransferases; DNA-Directed RNA Polymerases; Drug Stability; Escherichia coli; Kinetics; Nucleic Acid Denaturation; Nucleic Acid Hybridization; Polysaccharides; Sepharose; Temperature; Thymus Gland; Time Factors | 1975 |
Experimental optimization of the DASS system for immunodiagnosis of some helminth infections.
Topics: Alanine; Aminobutyrates; Aminohippuric Acids; Antigens; Citrulline; Cross Reactions; Cyanogen Bromide; Ethanolamines; Fasciola hepatica; Fascioliasis; Fluorescent Antibody Technique; Glutamates; Glycine; Humans; Phosphoric Acids; Schistosoma mansoni; Schistosomiasis; Sepharose; Serum Albumin, Bovine; Taurine | 1975 |
Isolation and purification of corticosteroid-binding globulin from human plasma by affinity chromatography.
Topics: Chromatography; Chromatography, Affinity; Cyanogen Bromide; Humans; Hydrocortisone; Hydroxyapatites; Methods; Sepharose; Succinates; Transcortin | 1975 |
Purification of catechol-O-methyltransferase by affinity chromatography.
Topics: Animals; Catechol O-Methyltransferase; Chromatography, Affinity; Chromatography, Gel; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Liver; Rats; Sepharose | 1975 |
Production of potent salmonella H antisera by immunization with flagellae, isolated by immunosorption.
Topics: Adsorption; Agglutination Tests; Animals; Antigens, Bacterial; Cyanogen Bromide; Flagella; Immune Sera; Immunization; Rabbits; Salmonella; Sepharose | 1975 |
Accessible hydrophobic groups of native proteins.
Topics: Amines; Binding Sites; Chromatography, Affinity; Cyanogen Bromide; Phenylalanine; Protein Binding; Protein Conformation; Sepharose; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet | 1975 |
On the properties of matrix bound lactate dehydrogenase.
Topics: Animals; Antigen-Antibody Reactions; Chickens; Complement Fixation Tests; Cyanogen Bromide; Dogfish; Isoenzymes; Kinetics; L-Lactate Dehydrogenase; Muscles; Myocardium; Protein Binding; Rabbits; Sepharose; Solubility | 1975 |
Affinity purification methods. Improved procedures for cyanogen bromide reaction on agarose.
Topics: Aminocaproates; Chromatography, Affinity; Cyanogen Bromide; Nitrogen; Polysaccharides; Pyrrolidinones; Sepharose; Solvents; Temperature; Time Factors | 1975 |
Application of spectrophotometric methods to the determination of protein bound to agarose beads.
Topics: Amino Acids; Binding Sites; Cyanogen Bromide; Evaluation Studies as Topic; Leucyl Aminopeptidase; Methods; Molecular Weight; Protein Binding; Sepharose; Solubility; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Trypsin | 1975 |
Stability of isoproterenol bound to cyanogen bromide-activated agarose.
Topics: Buffers; Chromatography, Affinity; Cyanogen Bromide; Drug Stability; Ethylenediamines; Isoproterenol; Ligands; Polysaccharides; Protein Binding; Sepharose | 1975 |
Cyanogen bromide and tryptic fragments of normal and haemophilic factor VIII.
Topics: Alkylation; Amino Acids; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Factor VIII; Hemophilia A; Humans; Immunoelectrophoresis; Isoelectric Focusing; Male; Oxidation-Reduction; Peptides; Plasmapheresis; Sepharose; Sodium Dodecyl Sulfate | 1975 |
Growth hormone covalently bound to sepharose or glass. Analysis of ligand release rates and characterization of soluble radiolabeled products.
Topics: Animals; Cattle; Chromatography, Gel; Cyanogen Bromide; Diazonium Compounds; Gamma Rays; Glass; Growth Hormone; Iodine Radioisotopes; Ligands; Methods; Polysaccharides; Radiation Effects; Sepharose; Temperature; Time Factors | 1975 |
Microscopic observations on commercial Sepharose: deviations from normal bead-structure.
Topics: Cyanogen Bromide; Evaluation Studies as Topic; Microscopy, Interference; Polysaccharides; Sepharose | 1975 |
A facile method for the preparation of N6-substituted ATP-Sepharose.
Topics: Adenosine Triphosphate; Binding Sites; Chromatography, Affinity; Cyanogen Bromide; Polysaccharides; Sepharose | 1975 |
[Comparison of the activities of free and carrier fixed horseradish peroxidase].
Topics: Binding Sites; Cyanogen Bromide; Dimethyl Sulfoxide; Formamides; Horseradish Peroxidase; Peroxidases; Sepharose; Solubility | 1975 |
Evidence for the activity of immobilised monomers of triose phosphate isomerase.
Topics: Animals; Binding Sites; Carbohydrate Epimerases; Chickens; Cyanogen Bromide; Enzyme Activation; Guanidines; Kinetics; Macromolecular Substances; Muscles; Protein Binding; Rabbits; Sepharose; Triose-Phosphate Isomerase | 1975 |
Solid-state protein kinase. A tool for post-synthetically modifying and radioactively labeling proteins in vitro.
Topics: Animals; Cyanogen Bromide; Histones; Isotope Labeling; Kinetics; Methods; Muscles; Phosphorus Radioisotopes; Protein Kinases; Proteins; Rats; Sepharose | 1975 |
Studies of the distribution of proteins bound to CNBr-activated Sepharose 6B at the electron-microscopic level.
Topics: Binding Sites; Cyanogen Bromide; Ferritins; Leucyl Aminopeptidase; Microscopy, Electron; Protein Binding; Proteins; Sepharose | 1975 |
Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction.
Topics: Binding Sites; Chromatography, Affinity; Concanavalin A; Cyanogen Bromide; Humans; Interferons; Polysaccharides; Protein Binding; Sepharose | 1976 |
Characterization of mineral-binding 40-kDa glycoprotein extracted from young adult rabbit alveolar bone.
Topics: Alveolar Process; Amino Acids; Animals; Blotting, Western; Chromatography, Agarose; Chromatography, High Pressure Liquid; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Mandible; Microbial Collagenase; Minerals; Molecular Weight; Osteonectin; Oxidation-Reduction; Rabbits; Sepharose; Silver; Sodium Dodecyl Sulfate | 1992 |
Comparative studies of agarose and kieselguhr-agarose composites for the preparation and operation of immunoadsorbents.
Topics: Animals; Antibodies, Monoclonal; Cyanogen Bromide; Diatomaceous Earth; Electrophoresis, Polyacrylamide Gel; Evaluation Studies as Topic; Humans; Hybridomas; Immunosorbent Techniques; Ligands; Sepharose; Sulfones | 1990 |
Compared stability of Sepharose-based immunoadsorbents prepared by various activation methods.
Topics: Animals; Antibodies; Antibody Specificity; Apolipoproteins B; Butylene Glycols; Chromatography, Affinity; Cyanogen Bromide; Goats; Humans; Hydrogen-Ion Concentration; Immunosorbent Techniques; Immunosorbents; Sepharose; Sulfones | 1992 |
Affinity chromatographic purification of immunoglobulin M antibodies utilizing immobilized mannan binding protein.
Topics: Animals; Antibodies, Monoclonal; Ascites; Carrier Proteins; Cattle; Chromatography, Affinity; Chromatography, High Pressure Liquid; Collectins; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Gels; Humans; Immunoglobulin G; Immunoglobulin M; Sepharose | 1992 |
Enhanced expression, purification, and characterization of a novel class alpha glutathione S-transferase isozyme appearing in rabbit hepatic cytosol following treatment with 4-picoline.
Topics: Amino Acid Sequence; Animals; Chromatography, Affinity; Chymotrypsin; Cyanogen Bromide; Cytosol; Electrophoresis, Polyacrylamide Gel; Glutathione; Glutathione Transferase; Isoenzymes; Liver; Male; Molecular Sequence Data; Picolines; Rabbits; Sepharose; Sequence Homology, Nucleic Acid | 1992 |
Immobilization of solubilized UDP-glucuronosyltransferase from rat liver microsomes to Sepharose 4B.
Topics: Animals; Cholic Acids; Cyanogen Bromide; Enzyme Stability; Glucuronosyltransferase; Hydrogen-Ion Concentration; Kinetics; Male; Microsomes, Liver; Rats; Rats, Inbred Strains; Sepharose; Substrate Specificity; Temperature | 1992 |
Preparation and application of an affinity matrix for phosphatidylinositol-specific phospholipase C.
Topics: Chromatography, Affinity; Cyanogen Bromide; Molecular Structure; Phosphatidylinositol Diacylglycerol-Lyase; Phosphoinositide Phospholipase C; Phosphoric Diester Hydrolases; Sepharose | 1990 |
Biochemical and functional characterization of human tissue-type plasminogen activator variants obtained by deletion and/or duplication of structural/functional domains.
Topics: Affinity Labels; Animals; Cell Line; Cloning, Molecular; Cricetinae; Cyanogen Bromide; DNA; DNA Restriction Enzymes; Escherichia coli; Fibrin; Fibrinogen; Fibrinolysis; Gene Expression; Humans; Mutation; Plasmids; Sepharose; Structure-Activity Relationship; Tissue Plasminogen Activator | 1990 |
Apocarboxypeptidase B-sepharose: a specific adsorbent for peptides.
Topics: Amino Acid Sequence; Apoenzymes; Apoproteins; Carboxypeptidase B; Carboxypeptidases; Chromatography, Affinity; Cyanogen Bromide; Endorphins; Enkephalins; Enzymes, Immobilized; Indicators and Reagents; Molecular Sequence Data; Peptides; Sepharose | 1990 |
Zinc modulates thrombin adsorption to fibrin.
Topics: Adsorption; Blood Coagulation Tests; Calcium; Chloromercuribenzoates; Chromatography, Affinity; Cyanogen Bromide; Fibrin; Fibrinogen; Humans; Iodine Radioisotopes; p-Chloromercuribenzoic Acid; Polyethylene Glycols; Protein Binding; Sepharose; Serum Albumin, Bovine; Thrombin; Zinc | 1990 |
Separation of T cell subpopulations by monoclonal antibodies and affinity chromatography.
Topics: Animals; Antibodies, Monoclonal; Cell Separation; Chromatography, Affinity; Cyanogen Bromide; Dextrans; Humans; Indicators and Reagents; Sepharose; T-Lymphocytes | 1986 |
Immobilization of pig liver microsomes. Stability of cytochrome P-450-dependent monooxygenase activities.
Topics: Animals; Carbon Monoxide; Cell Fractionation; Cyanogen Bromide; Cytochrome P-450 Enzyme System; Dextrans; Imidazoles; Microsomes, Liver; Mixed Function Oxygenases; Sepharose; Spectrophotometry; Swine | 1986 |
[Method of synthesizing affinity sorbents using cyanobrominated sepharose as a base].
Topics: Chemical Phenomena; Chemistry; Chromatography, Affinity; Cyanogen Bromide; Hydrogen-Ion Concentration; Sepharose | 1987 |
Immobilization of aspartate aminotransferase on agarose.
Topics: Aldehydes; Animals; Aspartate Aminotransferases; Binding Sites; Cyanogen Bromide; Cytosol; Enzymes, Immobilized; Kinetics; Myocardium; Sepharose; Sulfhydryl Compounds; Swine | 1989 |
[A method for immobilizing cytochrome P-450 LM2 while preserving its hydroxylating activity].
Topics: Animals; Catalysis; Cyanogen Bromide; Cytochrome P-450 Enzyme System; Endoplasmic Reticulum; Enzyme Activation; Enzymes, Immobilized; Hydroxylation; Isoenzymes; Liver; Methods; Rabbits; Sepharose; Spectrophotometry; Structure-Activity Relationship | 1989 |
Studies on the immobilization of glucuronidase (Part 1). Covalent immobilization on various carriers (a comparison).
Topics: Cyanogen Bromide; Enzyme Induction; Enzymes, Immobilized; Glass; Glucuronidase; Glutaral; Oxidation-Reduction; Periodic Acid; Sepharose | 1988 |
Immunoaffinity chromatography utilizing monoclonal antibodies. Factors which influence antigen-binding capacity.
Topics: Animals; Antibodies, Monoclonal; Antigen-Antibody Complex; Aspartate-Ammonia Ligase; Chromatography, Affinity; Cyanogen Bromide; Hydrogen-Ion Concentration; Indicators and Reagents; Ligases; Mice; Mice, Inbred BALB C; Sepharose | 1987 |
Immobilization of chicken liver fructose 1,6-bisphosphatase on CNBr-activated Sepharose.
Topics: Adenosine Monophosphate; Ammonia; Animals; Chickens; Cyanogen Bromide; Enzymes, Immobilized; Fructose-Bisphosphatase; Hydrogen-Ion Concentration; Liver; Potassium; Sepharose; Trypsin | 1985 |
Mixed enzymic reaction--internal diffusion kinetics of nonuniformly distributed immobilized enzymes. The system agarose-micrococcal endonuclease.
Topics: Cyanogen Bromide; Diffusion; Endonucleases; Enzymes, Immobilized; Gels; Kinetics; Micrococcus; Models, Biological; Sepharose; Spectrometry, Fluorescence | 1987 |
Affinity chromatography of fibrinogen on Lens culinaris agglutinin immobilized on CNBr-activated sepharose: study of the active groups involved in nonspecific adsorption.
Topics: Adsorption; Binding Sites; Chromatography, Affinity; Cyanogen Bromide; Fibrinogen; Lectins; Plant Lectins; Sepharose | 1988 |
Preparation of high capacity affinity adsorbents using new hydrazino-carriers and their use for low and high performance affinity chromatography of lectins.
Topics: Animals; Borohydrides; Chromatography, Affinity; Chromatography, High Pressure Liquid; Cyanogen Bromide; Epoxy Compounds; Horseshoe Crabs; Hydrazines; Lectins; Ligands; Sepharose; Time Factors | 1986 |
Covalent coupling of neuralizing factors from Xenopus to Sepharose beads: no decrease of inducing activity.
Topics: Animals; Cell Membrane; Cyanogen Bromide; Ectoderm; Gastrula; Nervous System; Proteins; Ribonucleoproteins; Sepharose; Xenopus laevis | 1986 |
Properties of Sepharose-bound beta-lactamase from Enterobacter cloacae.
Topics: beta-Lactamase Inhibitors; beta-Lactamases; Cyanogen Bromide; Drug Stability; Enterobacter; Enterobacteriaceae; Enzymes, Immobilized; Floxacillin; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Sepharose | 1985 |
Radioimmunoassay of progesterone and estriol in plasma using antibodies immobilized onto protein A-Sepharose CL-4B.
Topics: Antibodies; Cyanogen Bromide; Estriol; Humans; Progesterone; Radioimmunoassay; Sepharose; Staphylococcal Protein A | 1985 |
Coenzyme A and affinity chromatography.
Topics: Acetyltransferases; Chromatography, Affinity; Coenzyme A; Cyanogen Bromide; Methods; Protein Binding; Sepharose; Tyrosine Transaminase | 1974 |
Lectins from Abrus precatorius and Ricinus communis. I. Immunochemical relationships between toxins and agglutinins.
Topics: Absorption; Animals; Antibody Specificity; Antitoxins; Carbon Radioisotopes; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Galactose; Glycyrrhiza; HeLa Cells; Immune Sera; Immunodiffusion; Lactose; Lectins; Leucine; Mercaptoethanol; Peptides; Plant Lectins; Plant Proteins; Plants, Medicinal; Plants, Toxic; Protein Biosynthesis; Rabbits; Ricinus; Sepharose; Sodium Dodecyl Sulfate; Toxins, Biological | 1974 |
Properties of the competence-inducing factor of Bacillus subtilis 168I-.
Topics: Bacillus subtilis; Binding Sites; Calcium; Cell Wall; Chromatography, Affinity; Chromatography, DEAE-Cellulose; Copper; Cyanogen Bromide; Deoxyribonucleases; DNA, Bacterial; Lysogeny; Magnesium; Mutation; Protein Binding; Sepharose; Time Factors; Transformation, Genetic; Zinc | 1974 |
Purification of human serum albumin by affinity chromatography.
Topics: Binding Sites; Buffers; Chromatography, Affinity; Chromatography, Gas; Cyanogen Bromide; Evaluation Studies as Topic; Fatty Acids; Humans; Hydrocarbons; Hydrogen-Ion Concentration; Immunodiffusion; Methods; Molecular Weight; Protein Binding; Sepharose; Serum Albumin; Sulfhydryl Compounds | 1974 |
Hydrophobic chromatography.
Topics: Alkylation; Bacterial Proteins; Chromatography, Affinity; Cyanogen Bromide; Escherichia coli; Glutamine; Glycogen Synthase; Phosphorylases; Protein Binding; Sepharose | 1974 |
Group-specific separation of glycoproteins.
Topics: Acrylamides; Chromatography, Affinity; Cyanogen Bromide; Glutaral; Glycoproteins; Glycosides; Hexosamines; Hexoses; Lectins; Methods; Sepharose | 1974 |
Use of thyroxine in affinity chromatography.
Topics: Chromatography, Affinity; Cyanogen Bromide; Humans; Methods; Protein Binding; Sepharose; Thyroxine; Thyroxine-Binding Proteins | 1974 |
The preparation and characterisation of a water-soluble coenzymically active dextran-NAD+.
Topics: Alcohol Oxidoreductases; Animals; Binding Sites; Chromatography, Gel; Cyanogen Bromide; Dextrans; Kinetics; L-Lactate Dehydrogenase; Molecular Weight; Muscles; NAD; Oxidation-Reduction; Rabbits; Saccharomyces cerevisiae; Sepharose; Solubility; Spectrophotometry, Ultraviolet; Time Factors; Water | 1974 |
Affinity chromatography of vertebrate collagenases.
Topics: Animals; Chromatography, Affinity; Collagen; Cyanogen Bromide; Humans; Methods; Microbial Collagenase; Peptide Fragments; Sepharose | 1974 |
Topics in the methodology of substitution reactions with agarose.
Topics: Chemical Phenomena; Chemistry; Chromatography, Affinity; Cyanogen Bromide; Hydrazines; Ligands; Macromolecular Substances; Periodic Acid; Polysaccharides; Sepharose; Succinimides | 1974 |
Purification of human hexosaminidases A and B by affinity chromatography.
Topics: Chromatography, Affinity; Chromatography, DEAE-Cellulose; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Female; Glucosamine; Hexosaminidases; Humans; Isoenzymes; Kinetics; Oxidation-Reduction; Placenta; Pregnancy; Sepharose; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet | 1974 |
The alpha-chymotrypsin-catalyzed hydrolysis of sepharose-bound L-phenylalanine 4-nitroanilide.
Topics: Anilides; Binding Sites; Buffers; Chymotrypsin; Cyanogen Bromide; Kinetics; Molecular Weight; Nitro Compounds; Phenylalanine; Protein Binding; Sepharose; Solubility; Spectrophotometry; Spectrophotometry, Ultraviolet; Time Factors | 1974 |
Purification of urease by affinity chromatography. II. Further studies on matrix characteristics, ligand specificity, and adsorbent capacity.
Topics: Adsorption; Aminobutyrates; Carbon Radioisotopes; Cell-Free System; Chemical Phenomena; Chemistry; Chromatography, Affinity; Cyanogen Bromide; Ethylenediamines; Hydroxylamines; Hydroxyurea; Indicators and Reagents; Ligands; Plants; Protein Binding; Proteus; Sepharose; Urease | 1974 |
Monosaccharides attached to agarose.
Topics: Chromatography, Affinity; Cyanogen Bromide; Glucosamine; Glycosides; Ligands; Monosaccharides; Polysaccharides; Sepharose | 1974 |
Proceedings: Covalently bound nicotinamide riboside diphosphate ester.
Topics: Alcohol Oxidoreductases; Binding Sites; Cyanogen Bromide; L-Lactate Dehydrogenase; Nicotinamide Mononucleotide; Ribonucleotides; Sepharose | 1974 |
[Carrier-bound cytochrome b5 as substrate for the ascorbate: ferricytochrome-b5-oxidoreductase from mammalian liver microsomes (author's transl)].
Topics: Animals; Ascorbic Acid; Binding Sites; Cyanogen Bromide; Cytochrome Reductases; Cytochromes; Hydrogen-Ion Concentration; Kinetics; Microsomes, Liver; Oxidation-Reduction; Protein Binding; Sepharose; Spectrophotometry; Trypsin | 1974 |
Some properties of ribonucleases from Aspergillus saitoi and seminal vesicles immobilized on sepharose 4B activated by cyanogen bromide.
Topics: Aspergillus; Cyanogen Bromide; Hydrogen-Ion Concentration; Kinetics; Male; Protein Binding; Ribonucleases; Seminal Vesicles; Sepharose; Urea | 1974 |
Binding of proteins to CNBr-activated sepharose 4B.
Topics: Animals; Autoradiography; Binding Sites; Cattle; Cyanogen Bromide; Fluoresceins; Iodine Radioisotopes; Ligands; Molecular Conformation; Molecular Weight; Peroxidases; Polysaccharides; Protein Binding; Protein Conformation; Proteins; Sepharose | 1974 |
Application of immobilized aspartate aminotransferase and immobilized aspartate aminotransferase-malate dehydrogenase coupled system to micro-assay of L-aspartic acid.
Topics: Animals; Aspartate Aminotransferases; Aspartic Acid; Binding Sites; Cyanogen Bromide; Cytoplasm; Evaluation Studies as Topic; Kinetics; Malate Dehydrogenase; Methods; Microchemistry; Mitochondria, Muscle; Myocardium; Protein Binding; Sepharose; Solubility; Spectrophotometry, Ultraviolet; Swine; Time Factors | 1974 |
The identification of tRNA isoacceptors fractionated by polyacrylamide gel electrophoresis.
Topics: Amino Acids; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Escherichia coli; RNA, Bacterial; RNA, Transfer, Amino Acyl; Sepharose; Time Factors | 1984 |
Immobilization of NAD kinase.
Topics: Cyanogen Bromide; Drug Stability; Enzymes, Immobilized; Hot Temperature; Indicators and Reagents; Kinetics; Methods; Phosphotransferases; Phosphotransferases (Alcohol Group Acceptor); Sepharose | 1984 |
Procedures for the analysis of cyanogen bromide-activated Sepharose or Sephadex by quantitative determination of cyanate esters and imidocarbonates.
Topics: Chemical Phenomena; Chemistry; Cyanates; Cyanogen Bromide; Dextrans; Imidoesters; Methods; Polysaccharides; Sepharose | 1981 |
[Comparison of methods for immobilizing preparations of pure antibodies and nonspecific immunoglobulins].
Topics: Antibodies; Antigen-Antibody Complex; Cyanogen Bromide; Immunoglobulins; Immunosorbents; Sepharose | 1982 |
Sepharose 4B as a matrix for affinity chromatography. A spin-labelling investigation using nitroxides as model ligands.
Topics: Chromatography, Affinity; Cyanogen Bromide; Electron Spin Resonance Spectroscopy; Nitrogen Oxides; Polysaccharides; Sepharose; Solvents; Spin Labels | 1980 |
Plasma therapy of primary rat mammary carcinoma: antitumor activity of tumor-bearer plasma adsorbed against inactivated CNBr sepharose or protein A-sepharose.
Topics: Animals; Cyanogen Bromide; Female; Immunosorbent Techniques; Mammary Neoplasms, Experimental; Rats; Sepharose; Staphylococcal Protein A | 1984 |
Hydrophobic chromatography of cells: adsorption and resolution on homologous series of alkylagaroses.
Topics: Adsorption; Animals; Cell Aggregation; Cell Separation; Cells; Chemical Phenomena; Chemistry, Physical; Chickens; Chromatography; Cyanogen Bromide; Cytological Techniques; Erythrocyte Membrane; Guinea Pigs; In Vitro Techniques; Lymphocytes; Mice; Mice, Inbred DBA; N-Acetylneuraminic Acid; Neuraminidase; Osmotic Fragility; Polylysine; Rabbits; Sepharose; Sialic Acids; Spleen | 1984 |
Purification of calmodulin by Ca2+-dependent affinity chromatography.
Topics: Calcium; Calmodulin; Chemical Phenomena; Chemistry; Chromatography, Affinity; Cyanogen Bromide; Drug Stability; Epoxy Compounds; Phenothiazines; Sepharose; Spectrophotometry | 1983 |
Immunochemical studies of the non-specific interactions of cyanogen bromide-activated macroporous agarose-based immunoadsorbents.
Topics: Amino Acids; Animals; Antigen-Antibody Complex; Cyanogen Bromide; Ethanolamines; Humans; Immunochemistry; Immunoglobulin G; Immunosorbents; Sepharose; Sheep; Time Factors | 1983 |
Subunit interactions in tyrosinase from frog epidermis in immobilized enzyme systems.
Topics: Animals; Catechol Oxidase; Cyanogen Bromide; Enzyme Activation; Enzymes, Immobilized; Epidermis; Guanidine; Guanidines; Monophenol Monooxygenase; Protein Conformation; Rana esculenta; Sepharose; Spectrometry, Fluorescence | 1981 |
A new approach to the general detection and measurement of proteinase and proteinase inhibitor activities.
Topics: Animals; beta-Glucosidase; Caseins; Cattle; Cyanogen Bromide; Endopeptidases; Methods; Milk; Protease Inhibitors; Sepharose; Succinimides | 1982 |
Sepharose-stearate as substrate for rat liver long-chain fatty acyl-CoA synthetase.
Topics: Animals; Coenzyme A Ligases; Cyanogen Bromide; Indicators and Reagents; Kinetics; Liver; Mass Spectrometry; Polysaccharides; Rats; Repressor Proteins; Saccharomyces cerevisiae Proteins; Sepharose | 1983 |
Antithrombin III-binding capacity of heparin-sepharose as a function of activation temperature and duration.
Topics: Antithrombin III; Chromatography, Affinity; Cyanogen Bromide; Heparin; Humans; Polysaccharides; Protein Binding; Sepharose | 1983 |
Normal frog gastrula extracellular materials serve as a substratum for normal and hybrid cell adhesion when covalently coupled with CNBr-activated Sepharose beads.
Topics: Animals; Cell Adhesion; Cyanogen Bromide; Extracellular Space; Female; Gastrula; Hybrid Cells; Male; Rana catesbeiana; Rana pipiens; Sepharose | 1981 |
Temperature-sensitive binding of solid phase C1q to aggregated human immunoglobulin G.
Topics: Chromatography, Affinity; Complement Activating Enzymes; Complement C1q; Cyanogen Bromide; Humans; Immunoglobulin G; Macromolecular Substances; Sepharose; Temperature | 1981 |
Functional and structural properties of immobilized subunits of Escherichia coli alkaline phosphatase.
Topics: Alkaline Phosphatase; Cyanogen Bromide; Enzymes, Immobilized; Escherichia coli; Kinetics; Macromolecular Substances; Peptide Fragments; Protein Binding; Sepharose; Zinc | 1980 |
Purification of thymidylate synthetase from enzyme-poor sources by affinity chromatography.
Topics: Animals; Cattle; Chromatography, Affinity; Cyanogen Bromide; Escherichia coli; Methotrexate; Methyltransferases; Molecular Weight; Sepharose; Thymidylate Synthase; Thymus Gland | 1980 |
A novel, highly sensitive and rapid assay system for proteolytic enzymes.
Topics: Animals; Caseins; Cattle; Cross-Linking Reagents; Cyanogen Bromide; Female; Kinetics; Mastitis, Bovine; Methods; Milk; Peptide Hydrolases; Protease Inhibitors; Sepharose | 1982 |
A new approach (cyano-transfer) for cyanogen bromide activation of Sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives.
Topics: Chemical Phenomena; Chemistry; Cyanogen Bromide; Hydrogen-Ion Concentration; Methods; Nitrogen; Polysaccharides; Resins, Plant; Sepharose | 1982 |
Cyanate from alkaline hydrolysis of cyanogen bromide-activated polysaccharides.
Topics: Cyanates; Cyanogen Bromide; Hydrogen-Ion Concentration; Kinetics; Papain; Polysaccharides; Protease Inhibitors; Sepharose | 1981 |
Immobilized derivatives of vitamin B12 coenzyme and its analogs.
Topics: Chromatography, Affinity; Cobamides; Cyanogen Bromide; Methods; Propanediol Dehydratase; Sepharose; Structure-Activity Relationship | 1980 |
A device for CNBr activation of agarose gels.
Topics: Chromatography, Affinity; Cyanogen Bromide; Polysaccharides; Sepharose | 1980 |
The immobilization of mitochondrial malate dehydrogenase on Sepharose beads and the demonstration of catalytically active subunits.
Topics: Animals; Cyanogen Bromide; Enzymes, Immobilized; Hydrogen-Ion Concentration; Kinetics; Macromolecular Substances; Malate Dehydrogenase; Mitochondria, Heart; Sepharose; Swine | 1981 |
Antigen purification by monoclonal antibody immunoaffinity chromatography.
Topics: Ammonium Sulfate; Animals; Antibodies, Monoclonal; Antigens; Blotting, Western; Chemical Precipitation; Chromatography, Affinity; Cyanogen Bromide; Immunoglobulins; Immunologic Techniques; Mice; Proteins; Sepharose | 1995 |
Use of an SDS-gel-separated protein band as a ligand for affinity chromatography: procedure and application to the purification of domain-specific antibodies against alpha-actinin.
Topics: Actinin; Animals; Antibodies; Antibody Specificity; Chickens; Chromatography, Affinity; Cyanogen Bromide; Detergents; Electrophoresis, Polyacrylamide Gel; Ligands; Molecular Weight; Muscle, Skeletal; Octoxynol; Polyethylene Glycols; Sepharose; Sodium Dodecyl Sulfate | 1995 |
Purification of antibodies using affinity chromatography.
Topics: Animals; Antibodies; Ascitic Fluid; Chromatography, Affinity; Cyanogen Bromide; Haptens; Rabbits; Rats; Sepharose | 1994 |
Removal of endotoxin from antibody preparations for clinical use. Assessment of polymyxin-sepharose CNBr affinity chromatography.
Topics: Antibodies, Monoclonal; Chromatography, Affinity; Cyanogen Bromide; Endotoxins; Evaluation Studies as Topic; Humans; Polymyxins; Sepharose | 1994 |
Immobilized carboxypeptidase N. A potent bioreactor and specific adsorbent for peptides.
Topics: Adsorption; Amino Acids; Bradykinin; Catalysis; Cobalt; Cyanogen Bromide; Enzymes, Immobilized; Humans; Hydrolysis; Lysine Carboxypeptidase; Peptides; Sepharose; Zinc | 1994 |
Effect of antigen-specific immunoadsorption on antibody kinetics in a rat model.
Topics: Alkaline Phosphatase; Animals; Antibodies; Antibody Specificity; Antigens; Cyanogen Bromide; Female; Immunization; Immunosorbent Techniques; Models, Biological; Rats; Rats, Sprague-Dawley; Sepharose | 1993 |
Selective removal of beta-lactoglobulin directly from cow's milk and preparation of hypoallergenic formulas: a bioaffinity method.
Topics: Allergens; Animals; Chromatography, Affinity; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Humans; Hydrogen-Ion Concentration; Infant; Infant Food; Lactoglobulins; Milk; Milk Hypersensitivity; Protein Binding; Sepharose | 1993 |
Two methods for the introduction of amino groups into agarose-based matrices: their use in immunoaffinity chromatography.
Topics: Amines; Antibodies, Monoclonal; Chromatography, Affinity; Cyanogen Bromide; Enzyme-Linked Immunosorbent Assay; Glutaral; Glycine; Humans; Indicators and Reagents; Polyethyleneimine; Sepharose; von Willebrand Factor | 1993 |
A comparison of the leakage of a monoclonal antibody from various immunoaffinity chromatography matrices.
Topics: Animals; Antibodies, Monoclonal; Biotin; Chromatography, Affinity; Cyanogen Bromide; Enzyme-Linked Immunosorbent Assay; Evaluation Studies as Topic; Follicle Stimulating Hormone; Follicle Stimulating Hormone, beta Subunit; Humans; Imidazoles; Immunosorbent Techniques; Mice; Rats; Sepharose; Thyrotropin | 1995 |
Antibodies released from immunoadsorbents: effect of support, activation and elution conditions.
Topics: Adsorption; Antibodies; Apolipoproteins B; Chromatography, Affinity; Cyanogen Bromide; Extracorporeal Circulation; Humans; Hyperlipoproteinemia Type II; Immunosorbents; Sepharose | 1996 |
Immunoaffinity chromatography.
Topics: Amines; Antibodies; Antibodies, Monoclonal; Antigens; Chromatography, Affinity; Cyanogen Bromide; Immunosorbent Techniques; Ligands; Molecular Structure; Proteins; Sepharose | 1996 |
In vivo and in vitro optimization of depletion of IgM and IgG xenoantibodies by immunoadsorption using cell membrane proteins.
Topics: Adsorption; Animals; Blood Platelets; Cell Membrane; Cell Separation; Cells, Cultured; Cyanogen Bromide; Cytotoxicity Tests, Immunologic; Electrophoresis, Polyacrylamide Gel; Endothelium, Vascular; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Humans; Immunoglobulin G; Immunoglobulin M; Immunosorbent Techniques; Immunosorbents; Macaca mulatta; Male; Membrane Proteins; Nephelometry and Turbidimetry; Radioligand Assay; Sepharose; Swine; Transplantation, Heterologous | 1997 |
Affinity chromatography as a means to study multienzyme complexes involved in murein synthesis.
Topics: Cell Membrane; Chromatography, Affinity; Cyanogen Bromide; Escherichia coli; Glycosyltransferases; Multienzyme Complexes; Peptidoglycan; Plasmids; Polyethylene Glycols; Sepharose | 1996 |
Immunochemical study of immunoglobulins bound to lactate dehydrogenase.
Topics: Aged; Aged, 80 and over; Amino Acid Sequence; Bence Jones Protein; Chromatography, Affinity; Cyanogen Bromide; Female; Humans; Immunoglobulin G; Immunohistochemistry; Isoenzymes; L-Lactate Dehydrogenase; Male; Middle Aged; Molecular Sequence Data; Protein Structure, Secondary; Sepharose | 1997 |
Continuous superporous agarose beds for chromatography and electrophoresis.
Topics: Animals; Cattle; Chromatography, Agarose; Chromatography, Gel; Cyanogen Bromide; Durapatite; Electrophoresis, Agar Gel; L-Lactate Dehydrogenase; Membranes, Artificial; Proteins; Sepharose; Spectrophotometry, Ultraviolet | 1999 |
The synthesis and evaluation of novel sialic acid analogues bound to matrices for the purification of sialic acid-recognising proteins.
Topics: Animals; Calcium; Chromatography, Affinity; Cyanogen Bromide; Leeches; Ligands; Liver; N-Acetylneuraminic Acid; Neuraminidase; Proteins; Rats; Recombinant Proteins; Sepharose; Sialyltransferases; Trypanosoma cruzi | 1999 |
Mass spectrometric characterization of a discontinuous epitope of the HIV envelope protein HIV-gp120 recognized by the human monoclonal antibody 1331A.
Topics: Amino Acid Sequence; Antibodies, Monoclonal; Antigen Presentation; Binding Sites, Antibody; CD13 Antigens; Cyanogen Bromide; HIV Envelope Protein gp120; HIV-1; Humans; Hydrolysis; Immunodominant Epitopes; Molecular Sequence Data; Peptide Fragments; Sepharose; Serine Endopeptidases; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2000 |
Some general methods of preparing affinity columns.
Topics: Carbon Dioxide; Carboxymethylcellulose Sodium; Cellulose; Chromatography, Affinity; Cyanogen Bromide; DNA; Glutaral; Nucleotides; Nylons; Polymers; Polynucleotides; Sepharose; Solubility | 1974 |
Immobilization of plant histaminase for medical applications.
Topics: Amine Oxidase (Copper-Containing); Cyanogen Bromide; Enzymes, Immobilized; Fabaceae; Food Hypersensitivity; Histamine; Plants; Plants, Medicinal; Sepharose | 2000 |
Immobilized earthworm fibrinolytic enzyme III-1 with carbonyldiimidazole activated-agarose.
Topics: Animals; Cattle; Creatine Kinase; Cyanogen Bromide; Enzyme Activation; Enzymes, Immobilized; Fibrin; Fibrinolytic Agents; Imidazoles; Immunoglobulin G; Oligochaeta; Sepharose; Serum Albumin; Substrate Specificity; Sulfhydryl Reagents | 2002 |
[Study on preparation of a wound healing agent: fibronectin].
Topics: Chromatography, Affinity; Cyanogen Bromide; Fibronectins; Gelatin; Humans; Male; Sepharose; Wound Healing | 2003 |
Optimisation of the coupled monoclonal antibody density for recombinant hepatitis B virus surface antigen immunopurification.
Topics: Antibodies, Monoclonal; Chromatography, Affinity; Cyanogen Bromide; Hepatitis B Surface Antigens; Immunosorbents; Ligands; Pichia; Recombinant Proteins; Sepharose | 2005 |
Comparative diagnostic potentiality of ELISA and dot-ELISA in prepatent diagnosis of experimental Fasciola gigantica infection in cattle.
Topics: Animals; Antibodies, Helminth; Antigens; Antigens, Helminth; Cattle; Cyanogen Bromide; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Fasciola; Fascioliasis; Glycoproteins; Goats; Immunoglobulin G; Lymnaea; Rabbits; Sensitivity and Specificity; Sepharose; Time Factors; Trematode Infections | 2005 |
Application of phenylboronic acid modified hydrogel affinity chips for high-throughput mass spectrometric analysis of glycated proteins.
Topics: Aluminum; Boronic Acids; Cyanogen Bromide; Fluorescence; Glycation End Products, Advanced; Glycoproteins; Hydrogels; Indicators and Reagents; Ligands; Mass Spectrometry; Protein Array Analysis; Proteomics; Sepharose; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrophotometry, Ultraviolet | 2007 |
Purification of sequence-specific DNA-binding proteins by affinity chromatography.
Topics: Animals; Binding, Competitive; Chromatography, Affinity; Chromatography, Agarose; Cyanogen Bromide; DNA; DNA-Binding Proteins; Electrophoresis, Agar Gel; Electrophoresis, Polyacrylamide Gel; Humans; Protein Binding; Sepharose | 2001 |
Affinity purification of natural ligands.
Topics: Chromatography, Affinity; Cyanogen Bromide; Ligands; Nucleic Acids; Protein Binding; Proteins; Sepharose; Sulfones | 2001 |
Immunoaffinity chromatography.
Topics: Antibodies; Antigens, Surface; Chromatography, Affinity; Cyanogen Bromide; Membrane Proteins; Sepharose | 2001 |
Purification of sequence-specific DNA-binding proteins by affinity chromatography.
Topics: Base Sequence; Chromatography, Affinity; Cyanogen Bromide; DNA; DNA-Binding Proteins; Oligonucleotides; Sepharose | 2001 |
Cysteine enhances activity and stability of immobilized papain.
Topics: Cross-Linking Reagents; Cyanogen Bromide; Cysteine; Enzyme Stability; Enzymes, Immobilized; Hot Temperature; Hydrogen-Ion Concentration; Kinetics; Osmolar Concentration; Papain; Sepharose | 2010 |
1-Cyano-4-dimethylamino pyridinium tetrafluoroborate as a cyanylating agent for the covalent attachment of ligand to polysaccharide resins.
Topics: Cyanogen Bromide; Ligands; Molecular Structure; Nitriles; Polysaccharides; Pyridinium Compounds; Sepharose | 1983 |
Combined use of irreversible binding and MRM technology for low- and ultralow copy-number protein detection and quantitation.
Topics: Amino Acid Sequence; Animals; Blood Proteins; Cattle; Cyanogen Bromide; Humans; Limit of Detection; Linear Models; Molecular Sequence Data; Peptide Fragments; Protein Binding; Proteomics; Sepharose; Trypsin | 2013 |
Towards the N-terminal acetylome: an N-terminal acetylated peptide enrichment method using CNBr-activated sepharose resin.
Topics: Acetylation; Chromatography, Liquid; Cyanogen Bromide; HeLa Cells; Humans; Peptides; Proteome; Reproducibility of Results; Sensitivity and Specificity; Sepharose; Tandem Mass Spectrometry | 2013 |
Coupling antibody to cyanogen bromide-activated sepharose.
Topics: Antibodies, Immobilized; Antibodies, Monoclonal; Chromatography, Affinity; Cyanogen Bromide; Resins, Synthetic; Sepharose | 2014 |
N-terminal protein characterization by mass spectrometry after cyanogen bromide cleavage using combined microscale liquid- and solid-phase derivatization.
Topics: Amino Acid Sequence; Chromatography, High Pressure Liquid; Cyanogen Bromide; Molecular Weight; Peptide Fragments; Peptides; Proteins; Proteolysis; Sepharose; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2014 |
Improving the catalytic properties of immobilized Lecitase via physical coating with ionic polymers.
Topics: Biotechnology; Catalysis; Coated Materials, Biocompatible; Cyanogen Bromide; Dextran Sulfate; Enzyme Stability; Enzymes, Immobilized; Hydrogen-Ion Concentration; Phospholipases A1; Polyethyleneimine; Recombinant Fusion Proteins; Sepharose | 2014 |
Enhanced activity and stability of papain immobilized on CNBr-activated sepharose.
Topics: Biocatalysis; Cyanogen Bromide; Enzyme Stability; Enzymes, Immobilized; Hydrogen-Ion Concentration; Kinetics; Papain; Recycling; Sepharose; Temperature | 2015 |
Immobilization of lipases on hydrophobic supports involves the open form of the enzyme.
Topics: Ascomycota; Bacterial Proteins; Burkholderia cepacia; Cyanogen Bromide; Enzymes, Immobilized; Fungal Proteins; Hydrophobic and Hydrophilic Interactions; Kinetics; Lipase; Osmolar Concentration; Protein Conformation; Sepharose | 2015 |
Stabilization of multimeric sucrose synthase from Acidithiobacillus caldus via immobilization and post-immobilization techniques for synthesis of UDP-glucose.
Topics: Acidithiobacillus; Bacterial Proteins; Biocatalysis; Biotechnology; Cyanogen Bromide; Enzyme Stability; Enzymes, Immobilized; Glucosyltransferases; Glycomics; Glycosyltransferases; Glyoxylates; Hydrogen-Ion Concentration; Protein Multimerization; Sepharose; Temperature; Uridine Diphosphate Glucose | 2018 |
Free and Immobilized Lecitase™ Ultra as the Biocatalyst in the Kinetic Resolution of (
Topics: Alcohols; Butyrates; Catalysis; Cyanogen Bromide; Enzymes, Immobilized; Esters; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Lipase; Phenylbutyrates; Sepharose; Solvents; Stereoisomerism | 2020 |