cyanine-dye-3 and 2--deoxycytidine-5--triphosphate

Here, we describe a method for directly transferring very small amounts of reaction products from one surface to another. The approach is illustrated using a T4 DNA polymerase reaction to extend primers hybridized to a surface-confined DNA template. Following the extension reaction, the resulting oligonucleotide is transferred to a product surface. The important results are that (1) the spatial registration of the product is preserved after transfer; (2) the same reactant surface can be used to generate and transfer multiple iterations of products; and (3) the reaction products are biologically active after transfer.

Topics: Biotin; Carbocyanines; Deoxycytosine Nucleotides; Dimethylpolysiloxanes; DNA-Directed DNA Polymerase; DNA, Single-Stranded; Fluorescent Dyes; Microscopy, Fluorescence; Streptavidin; Surface Properties; Viral Proteins

Topics: Carbocyanines; Deoxycytosine Nucleotides; DNA, Complementary; Oligonucleotide Array Sequence Analysis