curcumin and triphosphoric-acid

Chitosan nanoparticles (CS NPs) showed promising results in drug, vaccine and gene delivery for the treatment of various diseases. The considerable attention towards CS was owning to its outstanding biological properties, however, the main challenge in the application of CS NPs was faced during their size-controlled synthesis. Herein, ionic gelation reaction between CS and sodium tripolyphosphate (TPP), a widely used and safe CS cross-linker for biomedical application, was exploited by a microfluidic approach based on a staggered herringbone micromixer (SHM) for the synthesis of TPP cross-linked CS NPs (CS/TPP NPs). Screening design of experiments was applied to systematically evaluate the main process and formulative factors affecting CS/TPP NPs physical properties (mean size and size distribution). Effectiveness of the SHM-assisted manufacturing process was confirmed by the preliminary evaluation of the biological performance of the optimized CS/TPP NPs that were internalized in the cytosol of human mesenchymal stem cells through clathrin-mediated mechanism. Curcumin, selected as a challenging model drug, was successfully loaded into CS/TPP NPs (EE% > 70%) and slowly released up to 48 h via the diffusion mechanism. Finally, the comparison with the conventional bulk mixing method corroborated the efficacy of the microfluidics-assisted method due to the precise control of mixing at microscales.

Topics: Chitosan; Curcumin; Drug Carriers; Humans; Lab-On-A-Chip Devices; Mesenchymal Stem Cells; Nanoparticles; Polyphosphates

The aim of the present investigation is the development, optimization and characterization of curcumin-loaded hybrid nanoparticles of vanillin-chitosan coated with super paramagnetic calcium ferrite. The functionally modified vanillin-chitosan was prepared by the Schiff base reaction to enhance the hydrophobic drug encapsulation efficiency. Calcium ferrite (CFNP) nano particles were added to the vanillin modified chitosan to improve the biocompatibility. The vanillin-chitosan-CFNP, hybrid nanoparticle carrier was obtained by ionic gelation method. Characterizations of the hybrid materials were performed by XRD, FTIR,

Topics: Antineoplastic Agents; Benzaldehydes; Calcium Compounds; Cell Line; Cell Survival; Chitosan; Curcumin; Drug Carriers; Drug Liberation; Ferric Compounds; Humans; Hydrogen-Ion Concentration; Kinetics; Magnetic Fields; MCF-7 Cells; Nanoparticles; Particle Size; Polyphosphates; Surface Properties

Photodynamic therapy (PDT) is an effective therapy for cancers and is a minimally invasive therapy with low dark toxicity and limited side effects. PDT employs the combination of photosensitizers with a specific light source to produce reactive oxygen species (ROS) to damage tumor cells.. We fabricated nanoparticles encapsulating curcumin through crosslinking chitosan and tripolyphosphate (TPP). Additionally, the chitosan was conjugated to epidermal growth factor in order to target the epidermal growth factor receptor (EGFR), overexpressed on cancer cells. To investigate PDT using fabricated nanoparticles, we measured cell viabilities and ROS production in relation to EGFR-overexpressing gastric cancer cells and non-cancer gastric cells.. The targeting nanoparticles displayed a superior PDT effect in the cancer cell, with a resultant approximately fourfold decrease in the IC. These curcumin-encapsulated chitosan/TPP nanoparticles are a promising targeted-PDT against EGFR-overexpressing cancers.

Topics: Apoptosis; Cell Line, Tumor; Cell Survival; Chitosan; Curcumin; Epidermal Growth Factor; ErbB Receptors; Flow Cytometry; Humans; Interleukin-10; Nanoparticles; Necrosis; Photochemotherapy; Photosensitizing Agents; Polyphosphates; Reactive Oxygen Species; Spectroscopy, Fourier Transform Infrared; Superoxides

A chitosan (CS)-chlorogenic acid (CA) conjugate was successfully prepared through free-radical-induced protocols with a substitution of CA on CS of 103.5 mg/g. ATR-FTIR and

Topics: Antitussive Agents; Caco-2 Cells; Cell Proliferation; Cell Survival; Chitosan; Chlorogenic Acid; Curcumin; Drug Carriers; Drug Liberation; Drug Stability; Gels; Humans; Hydrophobic and Hydrophilic Interactions; Magnetic Resonance Spectroscopy; Microscopy, Electron, Transmission; Nanoparticles; Particle Size; Polymerization; Polyphosphates; Spectroscopy, Fourier Transform Infrared; Surface Properties

The aim of this study was to assess the feasibility of microparticles for dissolution enhancement and oral bioavailability of curcumin (Cur). Microparticles were prepared by the ionic crosslinking interaction with the use of tripolyphosphate (TPP) and chitosan (Cs). The physicochemical characteristics of microparticles were investigated. The in vivo performance was assessed by a pharmacokinetic study. The microparticles had an average diameter of 58.50 microm. Acceptable drug loading and encapsulation efficiency of microparticles were obtained to be 33.5% and 85.2%, respectively. Dissolution of Cur enhanced in the microparticles in comparison with pure drug. Drug release profile of Cur from microparticles fitted the first-order model. Microparticles provided improved pharmacokinetic parameters (Cmax 270.24 ng/ml, T(max) 1.30 h) in rats as compared with pure drug (C(max) 87.06 nglml, Tmax 0.66 h). The AUC value of microparticles was 8.4 fold that of the pure drug. The information from this study suggests that the developed microparticles successfully enhanced dissolution of the poorly water-soluble drug Cur, and eventually, improved its oral bioavailability effectively.

Topics: Animals; Antioxidants; Biological Availability; Cesium; Chitosan; Chromatography, High Pressure Liquid; Cross-Linking Reagents; Curcumin; Drug Compounding; Electrochemistry; Excipients; Hydrophobic and Hydrophilic Interactions; Male; Microscopy, Electron, Scanning; Nanoparticles; Particle Size; Polyphosphates; Rats; Rats, Wistar