curcumin has been researched along with crocin* in 6 studies
1 review(s) available for curcumin and crocin
Article | Year |
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[Sugar Chain Construction of Functional Natural Products Using Plant Glucosyltransferases].
Plant secondary product glycosyltransferases belong to family 1 of the glycosyltransferase superfamily and mediate the transfer of a glycosyl residue from activated nucleotide sugars to lipophilic small molecules, thus affecting the solubility, stability and pharmacological activities of the sugar-accepting compounds. The biotechnological application of plant glycosyltransferases in glycoside synthesis has attracted attention because enzymatic glycosylation offers several advantages over chemical methods, including (1) avoiding the use of harsh conditions and toxic catalysts, (2) providing strict control of regio-and stereo-selectivity and (3) high efficiency. This review describes the in vivo and in vitro glycosylation of natural organic compounds using glycosyltransferases, focusing on our investigation of enzymatic synthesis of curcumin glycosides. Our current efforts toward functional characterization of some glycosyltransferases involved in the biosynthesis of iridoids and crocin, as well as in the sugar chain elongation of quercetin glucosides, are described. Finally, I describe the relationship of the structure of sugar chains and the intestinal absorption which was investigated using chemoenzymatically synthesized quercetin glycosides. Topics: Animals; Biological Products; Carbohydrates; Carotenoids; Curcumin; Glucosyltransferases; Glycosides; Glycosylation; Intestinal Absorption; Iridoids; Molecular Conformation; Plants; Quercetin; Rats; Solubility | 2015 |
5 other study(ies) available for curcumin and crocin
Article | Year |
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Synergistic anticancer effects of curcumin and crocin on human colorectal cancer cells.
Curcumin, a polyphenol compound derived from the Curcuma longa L, and crocin, a hydrophilic carotenoid from Crocus Sativus Linnaeus, are traditionally used in food preparations in many countries and could act as chemopreventive compounds against several diseases, including cancer. In this study, the synergistic effect of curcumin and crocin was investigated for the first time on inducing apoptosis and suppressing colorectal cancer cells (SW-480 cell line).. MTT, Annexin V-FITC/PI, and DAPI staining tests were employed to evaluate cell viability and apoptosis induction, respectively. The combined effect of curcumin and crocin on the expression of genes involved in apoptosis and proliferation was quantified using real-time PCR. The combination therapy effect on cell cycle progression was also evaluated by flow cytometry. Based on the obtained results, curcumin and crocin treatment could cooperatively reduce cell viability and induce apoptosis in SW-480 cells by modulating the expression of Bax, Bcl-2, Caspase-3, Caspase-8, Caspase-9, Jak2, Stat3, and Akt1 genes. Besides, curcumin and crocin were able to synergistically increase the cell cycle arrest at the sub G1 phase, induce autophagy and decrease the clonogenic ability of SW-480 cells.. These results suggested that curcumin and crocin combination could be considered a more effective therapeutic strategy for inhibiting colorectal cancer. Topics: Apoptosis; Carotenoids; Cell Line, Tumor; Cell Proliferation; Colorectal Neoplasms; Curcumin; Humans | 2022 |
Synergistic effects of exosomal crocin or curcumin compounds and HPV L1-E7 polypeptide vaccine construct on tumor eradication in C57BL/6 mouse model.
Cervical cancer is the most common malignant tumor in females worldwide. Human papillomavirus (HPV) infection is associated with the occurrence of cervical cancer. Thus, developing an effective and low-cost vaccine against HPV infection, especially in developing countries is an important issue. In this study, a novel HPV L1-E7 fusion multiepitope construct designed by immunoinformatics tools was expressed in bacterial system. HEK-293T cells-derived exosomes were generated and characterized to use as a carrier for crocin and curcumin compounds. The exosomes loaded with crocin and curcumin compounds as a chemotherapeutic agent (ExoCrocin and ExoCurcumin) were used along with the L1-E7 polypeptide for evaluation of immunological and anti-tumor effects in C57BL/6 mouse model. In vitro studies showed that ExoCrocin and ExoCurcumin were not cytotoxic at a certain dose, and they could enter tumor cells. In vivo studies indicated that combination of the L1-E7 polypeptide with ExoCrocin or ExoCurcumin could produce a significant level of immunity directed toward Th1 response and CTL activity. These regimens showed the protective and therapeutic effects against tumor cells (the percentage of tumor-free mice: ~100%). In addition, both ExoCrocin and ExoCurcumin represented similar immunological and anti-tumor effects. Generally, the use of exosomal crocin or curcumin forms along with the L1-E7 polypeptide could significantly induce T-cell immune responses and eradicate tumor cells. Topics: Animals; Capsid Proteins; Carotenoids; Cell Line, Tumor; Curcumin; Drug Synergism; Exosomes; Female; HEK293 Cells; Humans; Mice; Mice, Inbred C57BL; Oncogene Proteins, Viral; Papillomavirus E7 Proteins; Papillomavirus Vaccines; Peptides; Recombinant Proteins; Treatment Outcome; Uterine Cervical Neoplasms | 2021 |
Supplementation of the BIOXcell extender with the antioxidants crocin, curcumin and GSH for freezing bull semen.
Semen cryopreservation is routine in cattle, but the results of artificial insemination need improvement. A strategy to these aims is the supplementation of the freezing extender with novel antioxidants. This study aimed at testing the natural antioxidants curcumin and crocin as supplements to the commercial extender BIOXcell for freezing semen from 8 Holstein bulls. We tested curcumin at 0.05 and 0.1 mM (CU0.05, CU0.1) and crocin at 0.5 and 1.5 mM (CR0.5, CR1.5), with 0.5 mM reduced glutathione (GSH0.5) as reference, and a control (CTL, without supplementation). The samples were evaluated post-thawing and after 5 h at 38 °C by CASA for motility and flow cytometry for viability, apoptotic, capacitation, acrosomal status, cytoplasmic and mitochondrial reactive oxygen species (ROS) production, and chromatin status (SCSA). Control and GSH0.5 showed similar results, possibly because of the good protection from BIOXcell. CU0.05 and CU0.1 showed little effects but increased cytoplasmic ROS production and motility ALH. CR0.5 and CR1.5 decreased viability and increased apoptotic features significantly post-thawing and after the incubation, resulting in lower motility (significant after the incubation) but decreasing SCSA %HDS (loose chromatin). Whereas crocin at these concentrations seems incompatible with BIOXcell, maybe because of a prooxidant activity, curcumin use merits further research, considering the elevation of ROS with no significant negative effects. Topics: Animals; Antioxidants; Carotenoids; Cattle; Cryopreservation; Culture Media; Curcumin; Glutathione; Male; Semen; Semen Preservation; Sperm Motility | 2021 |
Interaction of small molecules with human tyrosinase: A surface plasmon resonance and molecular docking study.
Topics: Binding Sites; Carotenoids; Curcumin; Enzyme Inhibitors; Enzymes, Immobilized; Humans; Hydroquinones; Kinetics; Molecular Docking Simulation; Monophenol Monooxygenase; Protein Binding; Protein Interaction Domains and Motifs; Protein Structure, Secondary; Pyrogallol; Recombinant Proteins; Surface Plasmon Resonance; Tannins | 2016 |
Evaluation of crocin and curcumin affinity on mushroom tyrosinase using surface plasmon resonance.
Tyrosinase inhibitors have potential applications in the cosmetics and food industries for preventing browning reactions and also as therapeutic drugs for neurodegenerative diseases such as Parkinson's. In this article, crocin and curcumin were evaluated as mushroom tyrosinase inhibitors. Results showed that, both compounds strongly inhibited the diphenolase activity than monophenolase. The IC50 values for diphenolase activity were estimated to be 0.11 mM and 0.18 mM for crocin and curcumin respectively. The binding kinetics of crocin and curcumin was studied with mushroom tyrosinase using surface plasmon resonance (SPR). Tyrosinase was immobilized on the gold surface of a Biacore sensor chip through amine coupling. Binding of inhibitors was analyzed by SPR without the need to further modify the surface or the use of other reagents. The binding constant KD (M) for mushroom tyrosinase obtained was 1.21×10(-4) M for crocin and 1.64×10(-4) M for curcumin, while showing a higher affinity for L-DOPA 1.95×10(-8) M, a substrate for tyrosinase (positive control). The study reveals the SPR sensor's ability to detect binding of the inhibitors. Topics: Agaricales; Carotenoids; Curcumin; Enzyme Inhibitors; Enzymes, Immobilized; Levodopa; Monophenol Monooxygenase; Protein Binding; Surface Plasmon Resonance | 2014 |