curcumin and citreoviridin

Porphyromonas gingivalis is a well-known Gram-negative bacterium that causes periodontal disease. The bacterium metabolizes amino acids and peptides to obtain energy. An ion gradient across its plasma membrane is thought to be essential for nutrient import. However, it is unclear whether an ion-pumping ATPase responsible for the gradient is required for bacterial growth. Here, we report the inhibitory effect of protonophores and inhibitors of a proton-pumping ATPase on the growth of P. gingivalis. Among the compounds examined, curcumin and citreoviridin appreciably reduced the bacterial growth. Furthermore, these compounds inhibited the ATPase activity in the bacterial membrane, where the A-type proton-pumping ATPase (A-ATPase) is located. This study suggests that curcumin and citreoviridin inhibit the bacterial growth by inhibiting the A-ATPase in the P. gingivalis membrane.

Topics: Aurovertins; Bacterial Proteins; Cell Membrane; Curcumin; Periodontal Diseases; Porphyromonas gingivalis; Proton Pump Inhibitors; Proton Pumps; Proton-Translocating ATPases

Topics: Animals; Aurovertins; Binding Sites; Biocatalysis; Cattle; Curcumin; Drug Synergism; Enzyme Inhibitors; Escherichia coli; Hydrolysis; Molecular Docking Simulation; Mutation; Protein Conformation; Protein Subunits; Proton-Translocating ATPases; Rotation