curcumin and chrysophanic-acid

Erhuang Quzhi Granules (EQG), the Chinese herbal compound, has demonstrated significant clinical efficacy in treating non-alcoholic fatty liver disease (NAFLD). The mechanism of this treatment has been shown to involve the nuclear factor kappa B (NF-κB)/nod-like receptor thermal protein domain associated protein 3 (NLRP3) pathway. However, research on the material basis of EQG against NAFLD is still in its primary stages. Following these considerations, this study predicted and screened the active ingredients of EQG using the absorption, distribution, metabolism, and excretion (ADME) property evaluation tool and molecular docking. Then the levels of these active ingredients in EQG were measured using ultra-high-performance liquid chromatography (UHPLC). The efficacy of the active ingredients and their mechanisms were validated through both in vivo and in vitro experiments. The results indicate that the collected 12 components have favorable metabolic stability, are safe, and have drug-like properties. Aloe-emodin (AE), rhein (RH), curcumin (CUR), emodin (EM), and chrysophanol (CP) showed better binding affinity with TNF-α and Caspase-1 proteins. UHPLC analysis revealed that EQG contains AE, RH, CUR, EM, and CP. Cellular experiments proved that all these five ingredients reduce the accumulation of lipids and reactive oxygen species. In animal models of NAFLD, AE, and RH significantly improved the pathological symptoms of steatosis and fibrosis and reduced the levels of pro-inflammatory factors via the NF-κB/NLRP3 pathway. The results reveal the active ingredients of EQG for treating NAFLD based on the NF-κB/NLRP3 pathway and lay the foundation for the clinical promotion of EQG.

Topics: Animals; Curcumin; Emodin; Inflammasomes; Molecular Docking Simulation; Molecular Structure; NF-kappa B; NLR Family, Pyrin Domain-Containing 3 Protein; Non-alcoholic Fatty Liver Disease

To identify the endophyte strain E8 with high activity from Curcuma wenyujin and study its secondary metabolites.. The strain E8 was identified by morphological observation and ITS sequence analysis. Manifold chromatographic methods were used to separate and purify the chemical constituents of fermentation broth from strain E8, and their structures were identified by physiochemical properties and spectral data.. The strain E8 belongs to P. oxalicum. Four compounds were isolated from the fermentation broth of this strain and elucidated as chrysophanol, emodin, secalonic acid A and beta-sitosterol.. The endophyte P. oxalicum was isolated from medicinal plant Curcuma wenyujin for the first time. Four compounds were first isolated from endophytic fungus in C. wenyujin. Thus, microbial fermentation is a new access for these compounds production.

Topics: Anthraquinones; Curcuma; Emodin; Fermentation; Penicillium; Sitosterols; Xanthones

To study the releasing rate of chrysophanol from Ruyi Jinhuang plaster.. Based on the detecting methods of releasing rate and residual dosage applied in (Pharmacopoeia of P. R. C) (2000 edition), the contents of chrysophanol were detected by HPLC.. Chrysophanol in Ruyi Jinhuang plaster could well be released from plaster and to form medicinal concentration gradient.. Chrysophanol in Ruyi-Jinhuang plaster can possibly be absorbed by skin.

Topics: Administration, Cutaneous; Animals; Anthraquinones; Curcuma; Drug Combinations; Drugs, Chinese Herbal; Female; Guinea Pigs; Male; Phellodendron; Plants, Medicinal; Rheum; Skin; Skin Absorption