curcumin and ceric-oxide

Decellularized extracellular matrix (ECM) has been widely used for wound healing. But, ECM failed to integrate tissue and restore the tissue function properly, when elevated levels of free radicals and biofilm formation occur at the wound site. Here, nanoemulgel systems were fabricated, considering the combinatorial approach of nanotechnology (nanoceria and curcumin nanoemulsion) and ECM gel of goat small intestine submucosa. The curcumin was encapsulated in the nanoemulgel system to enhance bioavailability in terms of antibacterial, antioxidant, sustained release and permeation at the wound site. Nanoceria was also incorporated to enhance the antibacterial, antioxidant and wound healing properties of the fabricated nanoemulgel formulation. All the formulations were porous, hydrophilic, biodegradable, antioxidant, antibacterial, hemocompatible, biocompatible, and showed enhanced wound healing rate. The formulation (DG-SIS/Ce/NC) showed the highest free radicals scavenging capacity and antibacterial property with prolonged curcumin release (62.9% in 96 h), skin permeability (79.7% in 96 h); showed better cell growth under normal and oxidative-stressed conditions: it also showed full-thickness wound contraction (97.33% in 14 days) with highest collagen synthesis at the wound site (1.61 μg/mg in 14 days). The outcomes of this study suggested that the formulation (DG-SIS/Ce/NC) can be a potential nanoemulgel system for full-thickness wound healing application.

Topics: Anti-Bacterial Agents; Antioxidants; Cerium; Curcumin; Decellularized Extracellular Matrix; Wound Healing

A water- and alcohol-soluble cerium oxide-curcumin conjugate was obtained by co-evaporation with poly(N-vinylpyrrolidone) (PVP). A nanocomposite consisting of hybrid organic-inorganic particles was stable in a wide range of pH values. Its properties were evaluated using nine cell lines: normal (MDBK, ST, Vero) and malignant (L929, T98G, HEp-2, A549, RIN-m5F, Hep G2). PVP-stabilised nanoceria was shown to inhibit autoxidation of curcumin, to enhance curcumin photostability, to promote bioaccumulation and to affect curcumin cytotoxicity and photocytotoxicity, depending on cell type, being more toxic to cancer cells in a selective manner. Under the conditions of UVA/UVC or H

Topics: Animals; Antineoplastic Agents; Biological Availability; Cell Line, Tumor; Cell Survival; Cerium; Curcumin; Humans; Oxidative Stress; Ultraviolet Rays

During injury or diseased condition, wound dressing fails to properly integrate or repair the tissue and restore its function due to various factors like poor bioavailability, systemic delivery of hydrophobic drugs and elevated levels of reactive oxygen species. Here, we fabricated a novel nano-hybrid hydrogel system, based-on gelatin and oxidized dextran, embedded with nano-formulation of curcumin and cerium oxide, dispersed by physical interaction within the hydrogel. The curcumin was entrapped in amphiphilic alkylated-dextran nanoparticles to enhance its bioavailability and release at the injured site while cerium oxide nanoparticles were used without any additional processing. The hydrogel was characterized for various properties and demonstrated a controlled and prolonged drug release (∼63 % in 108 h), accelerated cell migration besides providing a highly significant antioxidant and in-vivo anti-inflammatory activity (∼39 %). The preliminary study suggests that this hybrid system can significantly promote wound healing and the potential to become an ideal wound dressing.

Topics: Cerium; Curcumin; Dextrans; Hydrogels; Nanoparticles; Wound Healing

Scarless healing of injury remains a clinical challenge because of its complicated and overlapping phases of inflammation, clearing, and regeneration. Curcumin has been already established as a potential wound healing agent for normal and diabetic-impaired wounds. Herein, the question has been addressed whether a well-known antioxidant cerium oxide nanoparticle (CNP) can potentiate the activity of curcumin to promote a cellular program for scarless healing. In this study, we have developed a biocompatible poly (acrylamide) hydrogel (PAGE)-based dressing material comprising of CNP and curcumin (ACC) and tested its wound healing activity in an animal model of acute wound. Characterization of the CNP- and curcumin-entrapped hydrogel dressing (ACC) demonstrated high loading efficiency and sustained release of curcumin. In a full-thickness acute wound healing model of rat, a single application of ACC dressing demonstrated higher wound healing efficacy (78%) and negligible scarring compared to dressings containing only curcumin or CNP in 7 days. Enhanced cell proliferation, higher collagen content, advanced wound maturity, re-epithelialization, and granulation tissue formation were observed using the combination of curcumin and CNP (ACC). Study of cellular mechanisms identified MCP-1 and TGF-β as the key drivers of differential and accelerated healing observed in the ACC group. These, coupled with the upregulation of growth-related signaling pathways (HER2/ErbB2, TGF-β-Smad2/3, MAPK/ERK, AKT, and VEGF), promoted almost scarless healing in animals treated with ACC. The optimized combination of curcumin and CNP used in our study shows distinct advantage and can be a better agent for complete wound healing.

Topics: Animals; Cell Line; Cerium; Curcumin; Cytokines; Drug Carriers; Drug Liberation; Humans; Hydrogels; Male; Nanoparticles; Protein Kinases; Rats, Wistar; Skin; Wound Healing