curcumin has been researched along with bathocuproine* in 1 studies
1 other study(ies) available for curcumin and bathocuproine
Article | Year |
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Metal-mediated DNA damage induced by curcumin in the presence of human cytochrome P450 isozymes.
Although curcumin is known to exhibit antitumor activity, carcinogenic properties have also been reported. To clarify the potentiality of carcinogenesis by curcumin, we have examined whether curcumin can induce DNA damage in the presence of cytochrome P450 (CYP) using [32P]-5(')-end-labeled DNA fragments obtained from genes relevant to human cancer. Curcumin treated with CYP 2D6, CYP1A1, or CYP1A2 induced DNA damage in the presence of Cu(II). CYP2D6-treated curcumin caused base damage, especially at 5(')-TG-3('), 5(')-GC-3('), and GG sequences. The DNA damage was inhibited by both catalase and bathocuproine, suggesting that reactive species derived from the reaction of H(2)O(2) with Cu(I) participate in DNA damage. Formation of 8-oxo-7,8-dihydro-2(')-deoxyguanosine was significantly increased by CYP2D6-treated curcumin in the presence of Cu(II). Time-of- flight mass spectrometry demonstrated that CYP2D6 catalyzed the conversion of curcumin to O-demethyl curcumin. Therefore, it is concluded that curcumin may exhibit carcinogenic potential through oxidative DNA damage by its metabolite. Topics: Animals; Carcinogens; Catalase; Cattle; Chelating Agents; Chromatography, High Pressure Liquid; Curcumin; Cyclin-Dependent Kinase Inhibitor p16; Cytochrome P-450 CYP1A1; Cytochrome P-450 CYP1A2; Cytochrome P-450 CYP2D6; Cytochrome P-450 Enzyme System; Deoxyadenosines; DNA Damage; Free Radical Scavengers; Genes, ras; Humans; Isoenzymes; Mass Spectrometry; Metals; Phenanthrolines; Tumor Suppressor Protein p53 | 2002 |