curcumin and 4-nitrophenol

curcumin has been researched along with 4-nitrophenol* in 5 studies

Other Studies

5 other study(ies) available for curcumin and 4-nitrophenol

ArticleYear
Reduced graphene oxide nanosheets modified with nickel disulfide and curcumin nanoparticles for non-enzymatic electrochemical sensing of methyl parathion and 4-nitrophenol.
    Mikrochimica acta, 2019, 10-19, Volume: 186, Issue:11

    Topics: Curcumin; Electrochemical Techniques; Food Contamination; Fruit and Vegetable Juices; Graphite; Insecticides; Limit of Detection; Malus; Methyl Parathion; Nanoparticles; Nickel; Nitrophenols; Reproducibility of Results; Rivers; Solanum lycopersicum; Water Pollutants, Chemical

2019
Divinyl sulfone cross-linked cyclodextrin-based polymeric materials: synthesis and applications as sorbents and encapsulating agents.
    Molecules (Basel, Switzerland), 2015, Feb-19, Volume: 20, Issue:3

    The aim of this study was to evaluate the crosslinking abilities of divinyl sulfone (DVS) for the preparation of novel water-insoluble cyclodextrin-based polymers (CDPs) capable of forming inclusion complexes with different guest molecules. Reaction of DVS with native α-cyclodextrin (α-CD), β-cyclodextrin (β-CD) and/or starch generates a variety of homo- and hetero-CDPs with different degrees of crosslinking as a function of the reactants' stoichiometric ratio. The novel materials were characterized by powder X-ray diffraction, electron microscopy and for their sorption of phenol and 4-nitrophenol. They were further evaluated as sorbents with phenolic pollutants (bisphenol A and β-naphthol) and bioactive compounds (the hormone progesterone and curcumin). Data obtained from the inclusion experiments show that the degree of cross-linking has a minor influence on the yield of inclusion complex formation and highlight the important role of the CDs, supporting a sorption process based on the formation of inclusion complexes. In general, the inclusion processes are better described by a Freundlich isotherm although an important number of them can also be fitted to the Langmuir isotherm with R2 ≥ 0.9, suggesting a sorption onto a monolayer of homogeneous sites.

    Topics: Adsorption; Benzhydryl Compounds; Cross-Linking Reagents; Curcumin; Cyclodextrins; Hydrogen-Ion Concentration; Naphthols; Nitrophenols; Phenols; Polymers; Progesterone; Starch; Sulfones; X-Ray Diffraction

2015
Relationships between p53 status, apoptosis and induction of micronuclei in different human and mouse cell lines in vitro: Implications for improving existing assays.
    Mutation research. Genetic toxicology and environmental mutagenesis, 2015, Volume: 789-790

    Accumulated evidence has shown that in vitro mammalian cell genotoxicity assays produce high frequencies of "misleading" positive results, i.e. predicted hazard is not confirmed in in vivo and/or carcinogenicity studies [1], raising the question of relevance to human risk assessment. A recent study of micronucleus (MN) induction [2] showed that commonly used p53-deficient rodent cell lines (CHL, CHO and V79) gave a higher frequency of "misleading" positive results with 9 non-DNA reactive, Ames-negative and in vivo negative chemicals [3] than human p53-competent cells (blood lymphocytes, TK6 and HepG2 cell lines). This raised the question of whether these differences were due to p53 status or species origin. This present study compared human versus mouse and p53-competent versus p53-mutated function. The same 9 chemicals were tested for induction of MN in mouse lymphoma L5178Y (mutated p53), human TK6 (functional p53) and WIL2-NS (TK6 related, with mutated p53) cells. Six chemicals provided clear positive increases in MN frequency in at least one cell type. L5178Y cells yielded clear positive responses with more chemicals than either TK6 or WIL2-NS, indicating origin rather than p53 functionality was most relevant. Apoptosis induction (measured via caspase-3/7) was also investigated with clear differences in the timing and extent of apoptosis induction between mouse and human cells noted. With curcumin in TK6 cells, induction of caspase-3/7 activity coincided with MN induction, whereas for L5178Y cells, MN induction occurred in the absence of increased caspase activity. By contrast, with MMS in TK6 cells, MN induction preceded increased caspase-3/7 activity. These data suggest that MN induction by "misleading positive" genotoxins in p53-competent human cell lines may result from apoptosis, whereas in p53-defective rodent cells such as L5178Y, MN induction may be independent of apoptosis.

    Topics: Acrylates; Animals; Apoptosis; Cell Line; Cell Line, Tumor; Cell Survival; Chlorophenols; Curcumin; Cytochalasin B; DNA Damage; Dose-Response Relationship, Drug; Eugenol; Lymphocytes; Mice; Micronucleus Tests; Mutation; Nitrophenols; Organic Chemicals; ortho-Aminobenzoates; Phthalic Anhydrides; Propyl Gallate; Reproducibility of Results; Resorcinols; Tumor Suppressor Protein p53

2015
A single amino acid in the PSPG-box plays an important role in the catalytic function of CaUGT2 (Curcumin glucosyltransferase), a Group D Family 1 glucosyltransferase from Catharanthus roseus.
    FEBS letters, 2007, Jun-12, Volume: 581, Issue:14

    Curcumin glucosyltransferase (CaUGT2) isolated from cell cultures of Catharanthus roseus exhibits unique substrate specificity. To identify amino acids involved in substrate recognition and catalytic activity of CaUGT2, a combination of domain swapping and site-directed mutagenesis was carried out. Exchange of the PSPG-box of CaUGT2 with that of NtGT1b (a phenolic glucosyltransferase from tobacco) led to complete loss of enzyme activity in the resulting recombinant protein. However, replacement of Arg378 of the NtGT1b PSPG-box with cysteine, the corresponding amino acid in CaUGT2, restored the catalytic activity of the chimeric enzyme. Further site-directed mutagenesis revealed that the size of the amino acid side-chain in that particular site is critical to the catalytic activity of CaUGT2.

    Topics: Amino Acid Sequence; Amino Acid Substitution; Arginine; Catalysis; Catalytic Domain; Catharanthus; Curcumin; Cysteine; Glucosyltransferases; Kinetics; Molecular Sequence Data; Molecular Structure; Mutagenesis, Site-Directed; Nitrophenols; Phylogeny; Plant Proteins; Sequence Homology, Amino Acid; Substrate Specificity

2007
Modification by curcumin of mutagenic activation of carcinogenic N-nitrosamines by extrahepatic cytochromes P-450 2B1 and 2E1 in rats.
    Cancer science, 2006, Volume: 97, Issue:9

    To elucidate the mechanism underlying suppression by curcumin of esophageal carcinogenesis induced by NMBA, we evaluated the CYP level and mutagenic activation of environmental carcinogens, by immunoblot analyses and Ames preincubation test, respectively, and bilirubin, 4-nitrophenol and testosterone UDPGT activities in F344 rats treated with curcumin and/or NMBA. No significant alterations in the hepatic levels of constitutive CYP proteins, mutagenic activation by liver S9 or hepatic UDPGT activities were produced by subcutaneous treatment with 0.5 mg/kg NMBA for 5 weeks and/or feeding of 0.05% and 0.2% curcumin for 6 weeks. In contrast, gavage of 0.2% curcumin decreased esophageal CYP2B1 and 2E1 by up to 60%, compared with vehicle control. Similarly, intragastric treatment with 270 mg/kg curcumin decreased esophageal and gastric CYP2B1 and CYP2E1, but not in lung, kidney or intestine. Conversely, large intestinal CYP2B1 was 2.8-fold higher in the treated rats than in control rats. Mutagenic activities of NOC, including NMBA, in the presence of esophagus and stomach S9 were markedly decreased in the treated rats, whereas those in the presence of large intestine S9 were 2.2-3.0-fold above control. These results show that modifying effects of curcumin on esophageal carcinogenesis can be attributed to a decrease in metabolic activation of NMBA by esophageal CYP2B1 during the initiation phase, without the contribution of metabolic activation and inactivation by liver. Further, the present findings suggest the potential of curcumin for modification of gastric and intestinal carcinogenesis initiated with NOC.

    Topics: Animals; Antineoplastic Agents; Bilirubin; Blotting, Western; Carcinogens; Curcumin; Cytochrome P-450 CYP2B1; Cytochrome P-450 CYP2E1; Dimethylnitrosamine; Esophagus; Glucuronosyltransferase; Intestines; Liver; Male; Mutagenicity Tests; Nitrophenols; Nitrosamines; Rats; Rats, Inbred F344; Stomach; Testosterone; UDP-Glucuronosyltransferase 1A9

2006