curcumin and 2--7--bis(carboxyethyl)-5(6)-carboxyfluorescein

curcumin has been researched along with 2--7--bis(carboxyethyl)-5(6)-carboxyfluorescein* in 1 studies

Other Studies

1 other study(ies) available for curcumin and 2--7--bis(carboxyethyl)-5(6)-carboxyfluorescein

ArticleYear
Modulatory effects of curcumin on multi-drug resistance-associated protein 5 in pancreatic cancer cells.
    Cancer chemotherapy and pharmacology, 2011, Volume: 68, Issue:3

    Chemotherapy of pancreatic cancer often fails due to the development of intrinsic and acquired resistance during drug treatment. Recent studies have suggested that MRP5 conferred resistance to first-line drugs 5-fluorouracil and gemcitabine by active efflux of drugs from the cell. Our aim was to evaluate whether curcumin could reverse this multi-drug resistance by inhibition of MRP5-mediated efflux.. MRP5 protein was detected and localized by immunocytochemistry using a monoclonal antibody in MRP5 over-expressing HEK293 (HEK293/MRP5) cells and two pancreatic cancer cell lines PANC-1 and MiaPaCa-2. The cellular accumulation of a specific MRP5 fluorescent substrate 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) into these cells was measured by flow cytometry and the cell proliferation determined by a 72-h CyQuant assay.. The cellular accumulation of BCECF in HEK293/MRP5 cells and in PANC-1 and MiaPaCa-2 cells was significantly increased by curcumin in a concentration-dependent manner. Curcumin and a MRP5 inhibitor MK571 had no apparent effects on cellular accumulation of BCECF in parental HEK293 cells. In the proliferation assays, curcumin caused a concentration-dependant increase in the sensitivity to the cytotoxic drug 5-fluorouracil in HEK293/MRP5 cells, PANC-1 and MiaPaCa-2 pancreatic cancer cells, but not in parental HEK293 cells.. Our results suggest that curcumin is an inhibitor of MRP5 and may be useful in the reversal of multi-drug resistance in pancreatic cancer chemotherapy.

    Topics: Anticarcinogenic Agents; Antimetabolites, Antineoplastic; Cell Line, Tumor; Cell Survival; Curcumin; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Fluoresceins; Fluorescent Antibody Technique; Fluorescent Dyes; Fluorouracil; Genistein; Humans; Immunohistochemistry; Multidrug Resistance-Associated Proteins; Pancreatic Neoplasms

2011