cryptocaryone and pinocembrin

Six flavonoids named cryptogiones A-F, and nine known compounds were isolated from an ethanol extract of stems of Cryptocaryachingii. The structures of the compounds were elucidated by interpretation of comprehensive spectroscopic data and X-ray analysis. A majority of these flavonoids contained an acetic acid/lactone moiety, a possible taxonomic marker. Anti-inflammatory effects of the compounds were evaluated using in vitro assays. At 20 μM concentration, three compounds significantly inhibited TNFα-induced NF-кB activation and LPS-induced IL-1β expression.

Topics: Anti-Inflammatory Agents; Biomarkers; Cryptocarya; Crystallography, X-Ray; Dose-Response Relationship, Drug; Ethanol; Flavanones; Flavonoids; HeLa Cells; Humans; Inhibitory Concentration 50; Interleukin-1beta; Lipopolysaccharides; Molecular Conformation; NF-kappa B; Plant Stems; Pyrones; Tumor Necrosis Factor-alpha

Four new flavanones, cryptoflavanones A-D (1-4, resp.), together with eight known compounds, were isolated from the leaves of Cryptocarya chinensis. The structures of these new compounds were determined by spectral analyses. Among the isolated compounds, pinocembrin (5) and cryptocaryone (6) exhibited antituberculosis activity against Mycobacterium tuberculosis H(37) Rv strain in vitro with MIC values of 3.5 and 25.0 μg/ml, respectively.

Topics: Antitubercular Agents; Cryptocarya; Flavanones; Magnetic Resonance Spectroscopy; Microbial Sensitivity Tests; Models, Molecular; Molecular Structure; Mycobacterium tuberculosis; Plant Leaves; Pyrones