cortodoxone and onapristone

The actions of glucocorticoids are mediated by the glucocorticoid receptor (GR), which is activated upon ligand binding, and can alter the expression of target genes either by transrepression or transactivation. We have applied FRAP (fluorescence recovery after photobleaching) to quantitatively assess the mobility of the yellow fluorescent protein (YFP)-tagged human GR alpha-isoform (hGRalpha) in the nucleus of transiently transfected COS-1 cells and to elucidate determinants of its mobility. Addition of the high-affinity agonist dexamethasone markedly decreases the mobility of the receptor in a concentration-dependent manner, whereas low-affinity ligands like corticosterone decrease the mobility to a much lesser extent. Analysis of other hGRalpha ligands differing in affinity suggests that it is the affinity of the ligand that is a major determinant of the decrease in mobility. Similar results were observed for two hGRalpha antagonists, the low-affinity antagonist ZK98299 and the high-affinity antagonist RU486. The effect of ligand affinity on mobility was confirmed with the hGRalpha mutant Q642V, which has an altered affinity for triamcinolone acetonide, dexamethasone, and corticosterone. Analysis of hGRalpha deletion mutants indicates that both the DNA-binding domain and the ligand-binding domain of the receptor are required for a maximal ligand-induced decrease in receptor mobility. Interestingly, the mobility of transfected hGRalpha differs among cell types. Finally, the proteasome inhibitor MG132 immobilizes a subpopulation of unliganded receptors, via a mechanism requiring the DNA-binding domain and the N-terminal part of the ligand-binding domain. Ligand binding makes the GR resistant to the immobilizing effect of MG132, and this effect depends on the affinity of the ligand. Our data suggest that ligand binding induces a conformational change of the receptor which is dependent on the affinity of the ligand. This altered conformation decreases the mobility of the receptor, probably by targeting the receptor to relatively immobile nuclear domains with which it transiently associates. In addition, this conformational change blocks immobilization of the receptor by MG132.

Topics: Active Transport, Cell Nucleus; Animals; Bacterial Proteins; Cell Line; Cell Nucleus; Chloramphenicol O-Acetyltransferase; Chlorocebus aethiops; Corticosterone; Cortodoxone; COS Cells; Cysteine Endopeptidases; Cytoplasm; Dexamethasone; Fluorescent Dyes; Genes, Reporter; Gonanes; HeLa Cells; Humans; Hydrocortisone; Kidney; Leupeptins; Ligands; Luminescent Proteins; Microscopy, Confocal; Mifepristone; Multienzyme Complexes; Mutagenesis, Site-Directed; Photochemistry; Protease Inhibitors; Proteasome Endopeptidase Complex; Protein Binding; Protein Conformation; Protein Isoforms; Protein Structure, Tertiary; Receptors, Glucocorticoid; Recombinant Fusion Proteins; Sequence Deletion; Transfection; Triamcinolone Acetonide