concanavalin-a and oxatomide

The influence of anti-allergic drugs on lymphocyte function was investigated by examining the blastic activity and cytokine production of human peripheral blood leukocytes in response to concanavalin A stimulation in vitro. Addition of ketotifen, disodium cromoglycate and oxatomide did not influence peripheral blood leukocyte blastic activity even when high concentrations (10.0 micrograms/ml) of these drugs were added to cell cultures. However, azelastine and terfenadine caused inhibition of peripheral blood leukocyte activation. Interleukin-2, interleukin-3, interleukin-4 and interleukin-5 production from peripheral blood leukocytes was strongly suppressed when the cells were cultured in the presence of these agents. This suppression was observed even when lower concentrations (1.0 and 0.5 micrograms/ml) of these agents were added to the cultures.

Topics: Cells, Cultured; Concanavalin A; Cromolyn Sodium; Cytokines; Enzyme-Linked Immunosorbent Assay; Histamine H1 Antagonists; Humans; Interleukin-2; Interleukin-3; Interleukin-4; Interleukin-5; Ketotifen; Lymphocyte Activation; Phthalazines; Piperazines; T-Lymphocytes

The present study was carried out to evaluate the inhibitory effect of oxatomide and disodium cromoglycate (DSCG) on the histamine release from rat PEC and lung slices induced by antigen-antibody reaction, concanavalin A, compound 48/80 and A-23187. Oxatomide in the range of 1 microM to 10 microM inhibited the allergic histamine release from rat PEC and lung slices induced by antigen-antibody reaction in the presence of phosphatidyl-L-serine (PS). Although the inhibitory action of oxatomide was significant even at a concentration of 1 microM, the action did not increase with an increase in concentration of the drug. DSCG showed a concentration-dependent inhibition on the allergic histamine release from rat PEC and lung slices in the range of 10 microM to 100 microM. The histamine release from rat PEC induced by the combined treatment of concanavalin A and PS was inhibited by oxatomide (0.1 to 10 microM) and DSCG (10 to 100 microM). Oxatomide (up to 10 microM) showed no effect on the histamine release induced by compound 48/80, while DSCG in the range of 10 microM to 100 microM showed a concentration dependent inhibition of the release. Oxatomide at a concentration of 10 microM showed a significant inhibition on the histamine release induced by the calcium ionophore A-23187. On the other hand, DSCG had no effect on this reaction. Oxatomide at high concentrations did not stimulate the histamine release by itself.

Topics: Animals; Ascitic Fluid; Calcimycin; Concanavalin A; Cromolyn Sodium; Histamine H1 Antagonists; Histamine Release; In Vitro Techniques; Lung; Male; Mast Cells; p-Methoxy-N-methylphenethylamine; Piperazines; Rats; Rats, Inbred Strains