concanavalin-a has been researched along with methylthymidine* in 2 studies
2 other study(ies) available for concanavalin-a and methylthymidine
| Article | Year |
|---|---|
Use of whole blood lymphocyte stimulation test for immunocompetency studies in bald eagles, red-tailed hawks, and great horned owls.
Mitogen-induced whole blood lymphocyte stimulation tests for immunocompetency studies in bald eagles (Haliaeetus leucocephalus), red-tailed hawks (Buteo jamaicensis), and great horned owls (Bubo virginianus) were developed. Combinations of incubation times, blood dilutions, concentrations of [3H]thymidine and [125I]2-deoxyuridine, antibiotics, phytohemagglutinin-P, and concanavalin A were tested for their effects on the stimulation index (SI). An antibiotic combination of gentamicin plus amphotericin B yielded low SI with lymphocytes from bald eagles, but not with lymphocytes from great horned owls or red-tailed hawks. Penicillin plus streptomycin caused no such depression of SI. Lymphocytes from all 3 species yielded maximum responses with a 48-hour prelabel and 12- to- 16 hour postlabel incubation period at 41 C and 1:20 blood dilution. Optimal mitogen concentrations for lymphocytes from bald eagles, red-tailed hawks, and great horned owls were 25 micrograms, 10 micrograms, and 10 micrograms of phytohemagglutinin-P/well, respectively, and 2.5 micrograms, 10 micrograms, and 10 micrograms of concanavalin A/well, respectively. Differences in SI were not seen between the 2 radioactive labels. The optimal concentration of the [3H]thymidine label ranged from 0.06 to 0.125 microCi/well. Topics: Amphotericin B; Animals; Birds; Concanavalin A; Female; Gentamicins; Idoxuridine; Immunocompetence; Lymphocyte Activation; Lymphocytes; Male; Penicillins; Phytohemagglutinins; Species Specificity; Streptomycin; Thymidine | 1984 |
Transformation of chicken lymphocytes stimulated by concanavalin A: response parameters.
The culture conditions for obtaining the maximum transformation of chicken lymphocytes stimulated by the mitogen Concanavalin A are described in this paper. Lymphocytes were cultured in round bottom microtiter tissue culture plates and the incorporation of 3H-methyl-thymidine (3H-TdR) into lymphocyte deoxyribonucleic acid was used to quantitatively evaluate transformation. Maximum incorporation of 3H-methyl-thymidine occurred when 2 x 10(6) lymphocytes and 4 micrograms of Concanavalin A were added to each culture. Deoxyribonucleic acid synthesis was greatest during the period of 18 to 36 hours after addition of mitogen. Differences between chicken and mammalian lymphocyte cultures are discussed. Topics: Animals; Chickens; Concanavalin A; Female; Humans; Lymphocyte Activation; Male; Thymidine | 1982 |