concanavalin-a and fasudil

concanavalin-a has been researched along with fasudil* in 2 studies

Other Studies

2 other study(ies) available for concanavalin-a and fasudil

Article	Year
Concanavalin A- and fetal-calf-serum-induced rounding and myosin light chain phosphorylation in cultured smooth muscle cells. Journal of cellular physiology, 1990, Volume: 144, Issue:2 Rabbit aorta smooth muscle cells (SMC) in long-term culture retracted in less than 10 min in response to a sequential order of stimulations by concanavalin A (Con A) and fetal calf serum (FCS). With additional continuous stimulation by FCS, the SMC took on a circular shape and were anchored to the substrate by retraction fibrils. This rounding was observed only when the cells were sequentially stimulated by Con A and FCS. Depletion of intracellular Ca2+ stores by the addition of EGTA and Ca2+ ionophores inhibited the rounding. Transient phosphorylation of MLC20 was observed in the initial stage during the SMC rounding. The extent of monophosphorylated MLC20 increased for up to 5 min to a maximal value of 49%. The diphosphorylated form reached a maximal value of 29% within 2 min; then both forms of MLC20 decreased. The process of the SMC rounding was inhibited by antimycin A or cytochalasins, in a dose-dependent manner, findings which suggested a dependency on both metabolic energy and actin-containing microfilaments. The smooth-muscle-relaxing agent, HA1077, also inhibited the process of SMC rounding. These observations suggest that a cellular contractile process might be involved in rounding of SMC. Topics: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; Animals; Aorta; Blood; Calcimycin; Calcium; Cells, Cultured; Chickens; Concanavalin A; Culture Media; Cytochalasin B; Cytochalasins; Diltiazem; Egtazic Acid; Gizzard, Avian; Ionomycin; Isoquinolines; Kinetics; Muscle, Smooth; Muscle, Smooth, Vascular; Myosin Subfragments; Phosphorylation; Rabbits; Verapamil	1990
Inhibition of myosin light chain phosphorylation in cultured smooth muscle cells by HA1077, a new type of vasodilator. Biochemical and biophysical research communications, 1990, Sep-28, Volume: 171, Issue:3 When cultured smooth muscle cells were stimulated sequentially by concanavalin A and fetal calf serum, the cells rounded up, and there was an accompanying mono- and diphosphorylation of the 20 kDa myosin light chain. HA1077, a new type of vasodilator, inhibited both the cell rounding and the light chain phosphorylation in a concentration dependent manner. Since HA1077 inhibits myosin light chain kinase, in vitro, we propose that this vasodilator presumably inhibits cell rounding by limiting myosin light chain phosphorylation. Topics: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; Animals; Aorta; Cells, Cultured; Concanavalin A; Isoquinolines; Kinetics; Muscle, Smooth, Vascular; Myosin Subfragments; Phosphorylation; Rabbits; Vasodilator Agents	1990

Article

Year

Concanavalin A- and fetal-calf-serum-induced rounding and myosin light chain phosphorylation in cultured smooth muscle cells.

Journal of cellular physiology, 1990, Volume: 144, Issue:2

Rabbit aorta smooth muscle cells (SMC) in long-term culture retracted in less than 10 min in response to a sequential order of stimulations by concanavalin A (Con A) and fetal calf serum (FCS). With additional continuous stimulation by FCS, the SMC took on a circular shape and were anchored to the substrate by retraction fibrils. This rounding was observed only when the cells were sequentially stimulated by Con A and FCS. Depletion of intracellular Ca2+ stores by the addition of EGTA and Ca2+ ionophores inhibited the rounding. Transient phosphorylation of MLC20 was observed in the initial stage during the SMC rounding. The extent of monophosphorylated MLC20 increased for up to 5 min to a maximal value of 49%. The diphosphorylated form reached a maximal value of 29% within 2 min; then both forms of MLC20 decreased. The process of the SMC rounding was inhibited by antimycin A or cytochalasins, in a dose-dependent manner, findings which suggested a dependency on both metabolic energy and actin-containing microfilaments. The smooth-muscle-relaxing agent, HA1077, also inhibited the process of SMC rounding. These observations suggest that a cellular contractile process might be involved in rounding of SMC.

Topics: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; Animals; Aorta; Blood; Calcimycin; Calcium; Cells, Cultured; Chickens; Concanavalin A; Culture Media; Cytochalasin B; Cytochalasins; Diltiazem; Egtazic Acid; Gizzard, Avian; Ionomycin; Isoquinolines; Kinetics; Muscle, Smooth; Muscle, Smooth, Vascular; Myosin Subfragments; Phosphorylation; Rabbits; Verapamil

1990

Inhibition of myosin light chain phosphorylation in cultured smooth muscle cells by HA1077, a new type of vasodilator.

Biochemical and biophysical research communications, 1990, Sep-28, Volume: 171, Issue:3

When cultured smooth muscle cells were stimulated sequentially by concanavalin A and fetal calf serum, the cells rounded up, and there was an accompanying mono- and diphosphorylation of the 20 kDa myosin light chain. HA1077, a new type of vasodilator, inhibited both the cell rounding and the light chain phosphorylation in a concentration dependent manner. Since HA1077 inhibits myosin light chain kinase, in vitro, we propose that this vasodilator presumably inhibits cell rounding by limiting myosin light chain phosphorylation.

Topics: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine; Animals; Aorta; Cells, Cultured; Concanavalin A; Isoquinolines; Kinetics; Muscle, Smooth, Vascular; Myosin Subfragments; Phosphorylation; Rabbits; Vasodilator Agents

1990