concanavalin-a has been researched along with ethylbenzene* in 1 studies
1 other study(ies) available for concanavalin-a and ethylbenzene
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Induction of rat alkaline phosphatase isozymes bearing a glycan-phosphatidylinositol anchor modified by in vivo treatment with a benzimidazole derivative linked to ethylbenzene.
Serum alkaline phosphatase (ALP) is detected in soluble-form as a result of translocation from the membrane site by cleavage at the glycosyl-phosphatidylinositol moiety (GPI anchor). It is known that membrane-bound ALP (mALP) can be detected in serum in certain pathological and physiological conditions, and that it can be solubilized in vitro to soluble-ALP (sALP) by phosphatidylinositol-specific phospholipase C (PIPLC), phospholipase D, bile salt, detergent, etc. We observed a marked increase in ALP activity in the serum of rats given a benzimidazole derivative by gavage, and detected it as slow-migrating ALPs (SM-ALPs), which were mALP-like but resistant to PIPLC and n-butanol treatment on disc PAGE. On the other hand, ficin treatment made SM-ALPs shift to the sALP position. The molecular size of the SM-ALPs was smaller than that of sALP on sodium dodecyl sulphide-polyacrylamide slab-gel electrophoresis (SDS-PAGE), and immunoreactivity revealed the intestinal type. SM-ALPs were also detected in the duodenum and jejunum. The main sugar chain structure of SM-ALPs was the biantennary complex-type, which was coincided with intestinal sALP sugar chain. These results suggest that intestinal ALPs induced by the benzimidazole derivative were modified in their C-terminus or GPI anchor region and modification of this region may also participate in translocation into the bloodstream. Topics: Alkaline Phosphatase; Animals; Benzene Derivatives; Benzimidazoles; Chromatography, Affinity; Concanavalin A; Duodenum; Electrophoresis, Polyacrylamide Gel; Glycosylphosphatidylinositols; Hot Temperature; Isoenzymes; Jejunum; Male; Phosphatidylinositol Diacylglycerol-Lyase; Phosphoinositide Phospholipase C; Precipitin Tests; Rats; Rats, Wistar; Type C Phospholipases | 2000 |