concanavalin-a has been researched along with erastin* in 1 studies
1 other study(ies) available for concanavalin-a and erastin
| Article | Year |
|---|---|
Membrane Damage during Ferroptosis Is Caused by Oxidation of Phospholipids Catalyzed by the Oxidoreductases POR and CYB5R1.
Ferroptosis is a form of necrotic cell death caused by iron-dependent peroxidation of polyunsaturated phospholipids on cell membranes and is actively suppressed by the cellular antioxidant systems. We report here that oxidoreductases, including NADPH-cytochrome P450 reductase (POR) and NADH-cytochrome b5 reductase (CYB5R1), transfer electrons from NAD(P)H to oxygen to generate hydrogen peroxide, which subsequently reacts with iron to generate reactive hydroxyl radicals for the peroxidation of the polyunsaturated fatty acid (PUFA) chains of membrane phospholipids, thereby disrupting membrane integrity during ferroptosis. Genetic knockout of POR and CYB5R1 decreases cellular hydrogen peroxide generation, preventing lipid peroxidation and ferroptosis. Moreover, POR knockdown in mouse liver prevents ConA-induced liver damage. Ferroptosis, therefore, is a result of incidental electron transfer carried out by POR/CYB5R1 oxidoreductase and thus needs to be constitutively countered by the antioxidant systems. Topics: Animals; Cell Line, Tumor; Cell Membrane; Concanavalin A; Cytochrome P-450 Enzyme System; Cytochrome-B(5) Reductase; Electron Transport; Fatty Acids, Unsaturated; Ferroptosis; HEK293 Cells; HeLa Cells; Humans; Hydrogen Peroxide; Lipid Peroxidation; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, Nude; NADP; Oxygen; Phenylurea Compounds; Piperazines; Pyridines; Sorafenib | 2021 |