concanavalin-a and dimazine

The effects of 1,1-dimethylhydrazine (UDMH) on several early events associated with lymphocyte activation were examined. The concentration of intracellular calcium ([Ca2+]i) and membrane potential of murine lymphocytes were found to be altered upon exposure to UDMH; [Ca2+]i was increased in murine thymocytes, while splenocytes exhibited membrane hyperpolarization. In addition, interleukin-2 receptor expression induced by in-vitro concanavalin A stimulation of murine splenocytes at 24 and 48 h in the presence of UDMH was not affected. UDMH may interfere with the ability of these two distinct lymphocyte populations to regulate normal ionic fluctuations, thus contributing to altered immune responsiveness.

Topics: Animals; Calcium; Cell Division; Concanavalin A; Dimethylhydrazines; Female; Flow Cytometry; Lymphocyte Activation; Lymphocytes; Membrane Potentials; Mice; Mice, Inbred BALB C; Receptors, Interleukin-2; Spleen; Thymus Gland; Time Factors