concanavalin-a and benoxaprofen

concanavalin-a has been researched along with benoxaprofen* in 2 studies

Other Studies

2 other study(ies) available for concanavalin-a and benoxaprofen

Article	Year
Benoxaprofen activation of suppressor activity in mononuclear leucocytes by a pro-oxidative mechanism in vitro. Agents and actions, 1984, Volume: 14, Issue:5-6 The effects of benoxaprofen on spontaneous and concanavalin A-induced suppressor activity in human mononuclear leucocytes (MNL) were assessed in vitro. The drug was used at a fixed concentration of 10(-4) M (30 micrograms/ml) in these studies. Benoxaprofen-treated MNL suppressed the responsiveness of untreated autologous MNL to the mitogen phytohaemagglutinin and potentiated the induction of suppressor activity in MNL by concanavalin A. Benoxaprofen at the same concentration increased MNL oxidative metabolism measured by chemiluminescence. Inclusion of the anti-oxidants ascorbate or cysteine (1 X 10(-3) M) in the assay system or depletion of adherent cells from MNL populations was associated with the elimination of both benoxaprofen-mediated suppression and increased MNL oxidative metabolism. Benoxaprofen per se was not an oxidizing agent nor did the drug possess peroxidase-like properties. These findings show that benoxaprofen induces suppressor activity in MNL by a pro-oxidative mechanism dependent upon intact cellular oxidative metabolism. Induction of suppressor activity in MNL by pro-oxidative drugs may be an important anti-inflammatory mechanism. Topics: Adult; Antioxidants; Concanavalin A; Humans; In Vitro Techniques; Luminescent Measurements; Lymphocyte Activation; Monocytes; Oxidation-Reduction; Phytohemagglutinins; Propionates; T-Lymphocytes, Regulatory	1984
Prevention of induction of suppressor activity in human mononuclear leukocytes by ascorbate and cysteine in vitro. International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition, 1983, Volume: 53, Issue:4 Concanavalin A (con A) and the non-steroidal anti-inflammatory drug benoxaprofen caused increased oxidative metabolism and suppressor activity in human mononuclear leukocytes in vitro. The relationship between enhanced oxidative metabolism and suppressor activity in MNL was investigated using the water-soluble anti-oxidants ascorbate and cysteine at fixed concentrations of 1 X 10(-3) M. Ascorbate and cysteine inhibited both the induction of suppressor activity and increased oxidative metabolism in MNL caused by con A and benoxaprofen. It is proposed that ascorbate and cysteine prevent the induction of non-specific suppressor activity in MNL by an anti-oxidant mechanism. Topics: Anti-Inflammatory Agents; Ascorbic Acid; Concanavalin A; Cysteine; Humans; Lymphocyte Activation; Lymphocytes; Oxidation-Reduction; Propionates; T-Lymphocytes, Regulatory	1983

Article

Year

Benoxaprofen activation of suppressor activity in mononuclear leucocytes by a pro-oxidative mechanism in vitro.

Agents and actions, 1984, Volume: 14, Issue:5-6

The effects of benoxaprofen on spontaneous and concanavalin A-induced suppressor activity in human mononuclear leucocytes (MNL) were assessed in vitro. The drug was used at a fixed concentration of 10(-4) M (30 micrograms/ml) in these studies. Benoxaprofen-treated MNL suppressed the responsiveness of untreated autologous MNL to the mitogen phytohaemagglutinin and potentiated the induction of suppressor activity in MNL by concanavalin A. Benoxaprofen at the same concentration increased MNL oxidative metabolism measured by chemiluminescence. Inclusion of the anti-oxidants ascorbate or cysteine (1 X 10(-3) M) in the assay system or depletion of adherent cells from MNL populations was associated with the elimination of both benoxaprofen-mediated suppression and increased MNL oxidative metabolism. Benoxaprofen per se was not an oxidizing agent nor did the drug possess peroxidase-like properties. These findings show that benoxaprofen induces suppressor activity in MNL by a pro-oxidative mechanism dependent upon intact cellular oxidative metabolism. Induction of suppressor activity in MNL by pro-oxidative drugs may be an important anti-inflammatory mechanism.

Topics: Adult; Antioxidants; Concanavalin A; Humans; In Vitro Techniques; Luminescent Measurements; Lymphocyte Activation; Monocytes; Oxidation-Reduction; Phytohemagglutinins; Propionates; T-Lymphocytes, Regulatory

1984

Prevention of induction of suppressor activity in human mononuclear leukocytes by ascorbate and cysteine in vitro.

International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition, 1983, Volume: 53, Issue:4

Concanavalin A (con A) and the non-steroidal anti-inflammatory drug benoxaprofen caused increased oxidative metabolism and suppressor activity in human mononuclear leukocytes in vitro. The relationship between enhanced oxidative metabolism and suppressor activity in MNL was investigated using the water-soluble anti-oxidants ascorbate and cysteine at fixed concentrations of 1 X 10(-3) M. Ascorbate and cysteine inhibited both the induction of suppressor activity and increased oxidative metabolism in MNL caused by con A and benoxaprofen. It is proposed that ascorbate and cysteine prevent the induction of non-specific suppressor activity in MNL by an anti-oxidant mechanism.

Topics: Anti-Inflammatory Agents; Ascorbic Acid; Concanavalin A; Cysteine; Humans; Lymphocyte Activation; Lymphocytes; Oxidation-Reduction; Propionates; T-Lymphocytes, Regulatory

1983