concanavalin-a and 5-(octadecylthiocarbamoylamino)fluorescein

In order to study the difference between normal and transformed cells, lateral motion of fluorescent molecules embedded into cell membranes of rat clonal fibroblasts and its SV40-transformed derivative cells was measured by the FPR technique. The lateral diffusion coefficient of a fluorescent fatty acid analog, F18, was smaller in transformed cells than normal cells. This indicates that the lipid phase of membranes from transformed cells is less fluid than that from normal cells. On the other hand, the lateral diffusion coefficient of S-F-concanavalin A was identical in both cells. These results suggest that the mobility of different molecules on the membranes is controlled by different mechanisms.

Topics: Animals; Cell Line, Transformed; Cell Membrane; Cell Transformation, Viral; Concanavalin A; Diffusion; Fibroblasts; Fluorescein-5-isothiocyanate; Fluoresceins; Fluorescent Dyes; Membrane Fluidity; Rats; Simian virus 40