concanavalin-a and 4-methylumbelliferyl-glucoside

'Acid' beta-glucosidase of human spleen, from either normal controls or patients with type 1 (adult) Gaucher disease, was incorporated into phosphatidylcholine liposomes. The non-incorporated (soluble) Gaucher-enzyme had a higher apparent molecular weight than had the corresponding control. Liposomal 'acid' beta-glucosidase prepared from Gaucher-spleen was more thermostable than was the corresponding normal enzyme; it was also stimulated by acidic lipids to a much lesser extent. The results suggest that the genetic mutation in type 1 (adult) Gaucher disease has multiple effects on the glycoprotein form of 'acid' beta-glucosidase.

Topics: beta-Glucosidase; Cell Membrane; Concanavalin A; Gaucher Disease; Glucosidases; Glucosides; Hot Temperature; Humans; Hydrogen-Ion Concentration; Hymecromone; Kinetics; Liposomes; Molecular Weight; Phosphatidylcholines; Solubility; Spleen

The rates of formation and dissociation of concanavalin A with some 4-methylumbelliferyl and p-nitrophenyl derivatives of alpha- and beta-D-mannopyranosides and glucopyranosides were measured by fluorescence and spectral stopped-flow methods. All processes examined were uniphasic. The second-order formation rate constants varied only from 6.8 x 10(4) to 12.8 x 10(4) M-1 x s-1, whereas the first-order dissociation rate constants ranged from 4.1 to 220 s-1, all at pH 5.0, I=0.3 M, and 25 degrees C. Dissociation rates thus controlled the value of the binding constant. The effect of temperature on these reactions was examined, from which enthalpies and entropies of activation and of reaction could be calculated. The effects of pH at 25 degrees C on the reaction rates of 4-methylumbelliferyl alpha-D-mannopyranoside and 4-methylumbelliferyl alpha-D-glucopyranoside with concanavalin A were examined. The value of the binding constant Kap (derived from the kinetics) at any pH could be related to the intrinsic binding constant K by the expression Kap = KaK(Ka + [H+])-1. The values of Ka, the ionization constant of the protein segment responsive to sugar binding, were 3 x 10(-4) M and 1 x 10(-4) M for 4-methylumbelliferyl alpha-D-mannopyranoside and 4-methylumbelliferyl alpha-D-glucopyranoside, respectively. The binding constant of p-nitrophenyl alpha-D-mannopyranoside is surprisingly much less sensitive to a pH change from 5.0 to 2.7. Ionic strength had little effect on the binding characteristics of 4-methylumbelliferyl alpha-D-mannopyranoside to concanavalin A at pH 5.2 and 25 degrees C.

Topics: Concanavalin A; Glucosides; Glycosides; Hydrogen-Ion Concentration; Hymecromone; Kinetics; Mannosides; Temperature; Thermodynamics