concanavalin-a has been researched along with 4-bromophenacyl-bromide* in 1 studies
1 other study(ies) available for concanavalin-a and 4-bromophenacyl-bromide
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Inhibitors of arachidonic acid metabolism eliminate the increase in cytosolic free calcium induced by the mitogen concanavalin A in rat thymocytes.
Using inhibitors of arachidonic acid (AA) metabolism, the possible involvement of AA products in the generation of [Ca2+]i and the pHi rise induced by the mitogen concanavalin A (Con A) in rat thymocytes has been studied. The lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA, 10 microM) and the phospholipase A2 inhibitor bromophenacyl bromide (10 microM) eliminated the [Ca2+]i signal induced by Con A; the cyclooxygenase blocker indomethacin also inhibited it. However, neither NDGA nor indomethacin suppressed the pHi rise stimulated by Con A. Exogenous AA induced an increase in [Ca2+]i but not in the pHi. These results indicate that AA metabolites, probably of the lipoxygenase pathway, take part in the generation of the [Ca2+]i response to the mitogen. In contrast, they appear not to be involved in the pHi rise evoked by Con A. Topics: Acetophenones; Animals; Arachidonic Acid; Arachidonic Acids; Catechols; Concanavalin A; Cytosol; Hydrogen-Ion Concentration; In Vitro Techniques; Indomethacin; Kinetics; Masoprocol; Phospholipases A; Phospholipases A2; Rats; Rats, Inbred Strains; T-Lymphocytes | 1989 |