concanavalin-a and 4-biphenylamine

The susceptibility of analbuminemic rats, a mutant strain of Sprague-Dawley rats, to bladder carcinogens was examined by testing the agglutinability of isolated bladder epithelial cells by Concanavalin A (Con A). One-week treatment with 0.001% N-butyl-N-(4-hydroxybutyl)nitrosamine (BHBN) in the drinking water or 0.01% N-(4-(5-nitro-2-furyl)-2-thiazolyl)-formamide (FANFT), 0.01% and 0.1% 4-aminobiphenyl, 0.1% 4-nitrobiphenyl, 0.1% benzidine, 0.1% 2-napthylamine or 5% sodium saccharin in the diet clearly induced high agglutinability of bladder cells of male analbuminemic rats but not Sprague-Dawley rats. These results suggest that male analbuminemic rats are very susceptible to bladder carcinogens in general and therefore will be very useful in screening tests for bladder carcinogens.

Topics: 2-Naphthylamine; Agglutination Tests; Aminobiphenyl Compounds; Animals; Butylhydroxybutylnitrosamine; Carcinogens; Cells, Cultured; Concanavalin A; Diet; Disease Susceptibility; Drug Evaluation, Preclinical; FANFT; Male; Rats; Rats, Inbred Strains; Saccharin; Serum Albumin; Urinary Bladder; Urinary Bladder Neoplasms