concanavalin-a and 3-3--dipropyl-2-2--thiadicarbocyanine

concanavalin-a has been researched along with 3-3--dipropyl-2-2--thiadicarbocyanine* in 2 studies

Other Studies

2 other study(ies) available for concanavalin-a and 3-3--dipropyl-2-2--thiadicarbocyanine

Article	Year
Fluorescence energy transfer-sensitized photobleaching of a fluorescent label as a tool to study donor-acceptor distance distributions and dynamics in protein assemblies: studies of a complex of biotinylated IgM with streptavidin and aggregates of concana Journal of photochemistry and photobiology. B, Biology, 1997, Volume: 40, Issue:3 A photokinetic method of detection of fluorescence resonance energy transfer (FRET) between special fluorescent labels is applied to study time-averaged spatial distribution of labeled proteins in protein assemblies. Prolonged irradiation of a sample at the absorption maximum of the energy donor label initiates FRET-sensitized fluorescence photobleaching of the energy acceptor label, which was monitored by steady-state fluorimetric measurements. Kinetics of the acceptor photobleaching and kinetics of decreasing the efficiency of FRET from donors to unbleached acceptors were determined. The FRET efficiency was found from measuring sensitization of acceptor fluorescence. Analysis of the photokinetic data permits to estimate the time-averaged distribution of acceptors on donor-acceptor distances in the range of characteristic distances of FRET. Dynamic processes influencing donor-acceptor distances can be also investigated by the method. Application of the method is demonstrated by the studies of a complex of biotinylated IgM with streptavidin and aggregates composed of concanavalin A and sodium dodecyl sulphate. A new thiadicarbocyanine dye was used as the acceptor label, R-phycoerythrin and tetramethylrhodamine isothio-cyanate were the donor labels. In the IgM-streptavidin complex, 16% of acceptors most contributed to FRET provided 90% of FRET efficiency, whereas acceptors made about the same time-averaged contribution to FRET in the concanavalin A aggregates. Topics: Animals; Benzothiazoles; Biotin; Biotinylation; Carbocyanines; Concanavalin A; Energy Transfer; Fluorescence; Fluorescent Dyes; Humans; Immunoglobulin M; Mice; Molecular Structure; Phycoerythrin; Rhodamines; Sodium Dodecyl Sulfate; Streptavidin	1997
[Effect of quinine, a blocker of Ca2+-activated K+-channels, on lymphocyte activation by mitogens]. Tsitologiia, 1986, Volume: 28, Issue:1 Quinine inhibits mitogenesis at the same concentration (10(-4) M) as that which blocks Ca++-dependent potassium transport in lymphocytes. Lower quinine concentrations (10(-8)-10(-6) M) induce a comitogenic effect which is most pronounced when Ca++-ionophore A23187 is used as a mitogen. Thus, activation of Ca++-dependent K+-channels is not necessary to trigger mitogenesis, but is important for further stages of the process. Topics: Benzothiazoles; Calcimycin; Calcium; Carbocyanines; Cell Membrane Permeability; Cells, Cultured; Concanavalin A; DNA; Fluorescent Dyes; Humans; Ion Channels; Lymphocyte Activation; Membrane Potentials; Mitogens; Phytohemagglutinins; Potassium; Quinine	1986

Article

Year

Fluorescence energy transfer-sensitized photobleaching of a fluorescent label as a tool to study donor-acceptor distance distributions and dynamics in protein assemblies: studies of a complex of biotinylated IgM with streptavidin and aggregates of concana

Journal of photochemistry and photobiology. B, Biology, 1997, Volume: 40, Issue:3

A photokinetic method of detection of fluorescence resonance energy transfer (FRET) between special fluorescent labels is applied to study time-averaged spatial distribution of labeled proteins in protein assemblies. Prolonged irradiation of a sample at the absorption maximum of the energy donor label initiates FRET-sensitized fluorescence photobleaching of the energy acceptor label, which was monitored by steady-state fluorimetric measurements. Kinetics of the acceptor photobleaching and kinetics of decreasing the efficiency of FRET from donors to unbleached acceptors were determined. The FRET efficiency was found from measuring sensitization of acceptor fluorescence. Analysis of the photokinetic data permits to estimate the time-averaged distribution of acceptors on donor-acceptor distances in the range of characteristic distances of FRET. Dynamic processes influencing donor-acceptor distances can be also investigated by the method. Application of the method is demonstrated by the studies of a complex of biotinylated IgM with streptavidin and aggregates composed of concanavalin A and sodium dodecyl sulphate. A new thiadicarbocyanine dye was used as the acceptor label, R-phycoerythrin and tetramethylrhodamine isothio-cyanate were the donor labels. In the IgM-streptavidin complex, 16% of acceptors most contributed to FRET provided 90% of FRET efficiency, whereas acceptors made about the same time-averaged contribution to FRET in the concanavalin A aggregates.

Topics: Animals; Benzothiazoles; Biotin; Biotinylation; Carbocyanines; Concanavalin A; Energy Transfer; Fluorescence; Fluorescent Dyes; Humans; Immunoglobulin M; Mice; Molecular Structure; Phycoerythrin; Rhodamines; Sodium Dodecyl Sulfate; Streptavidin

1997

[Effect of quinine, a blocker of Ca2+-activated K+-channels, on lymphocyte activation by mitogens].

Tsitologiia, 1986, Volume: 28, Issue:1

Quinine inhibits mitogenesis at the same concentration (10(-4) M) as that which blocks Ca++-dependent potassium transport in lymphocytes. Lower quinine concentrations (10(-8)-10(-6) M) induce a comitogenic effect which is most pronounced when Ca++-ionophore A23187 is used as a mitogen. Thus, activation of Ca++-dependent K+-channels is not necessary to trigger mitogenesis, but is important for further stages of the process.

Topics: Benzothiazoles; Calcimycin; Calcium; Carbocyanines; Cell Membrane Permeability; Cells, Cultured; Concanavalin A; DNA; Fluorescent Dyes; Humans; Ion Channels; Lymphocyte Activation; Membrane Potentials; Mitogens; Phytohemagglutinins; Potassium; Quinine

1986