communic-acid and isocupressic-acid

communic-acid has been researched along with isocupressic-acid* in 2 studies

Other Studies

2 other study(ies) available for communic-acid and isocupressic-acid

ArticleYear
Isolation of diterpenes from Araucaria sp Brazilian brown propolis and development of a validated high-performance liquid chromatography method for its analysis.
    Journal of separation science, 2021, Volume: 44, Issue:16

    Propolis comprises a complex resinous product composed of plant's parts or exudates, pollen, bee wax, and enzymes. Brazilian brown propolis from Araucaria sp displays several biological activities. Considering the lack of validated analytical methods for its analysis, we are reporting the development of a validated high-performance liquid chromatography with photodiode array detector method to analyze Araucaria brown propolis. The crude propolis were extracted and chromatographed, furnishing six main diterpenes. The isolated standards were used to draw the analytical curves, allowing the studies of selectivity, precision, accuracy, recovery, robustness, the determination of limits of detection and limits of quantification. The mobile phase consisted of 0.1% acetic acid in water and acetonitrile, using an octadecylsilane column, 1 mL/min flow rate and detection at 200 or 241 nm. Relative standard deviation values obtained for intra-day and inter-day precision were lower than 4% for all diterpenes. From the five parameters for robustness, wavelength detection and flow rate were the critical ones. Limits of detection and quantification ranged from 0.808 to 10.359 μg/mL and from 2.448 to 31.392 μg/mL, respectively. The recoveries were between 105.03 and 108.13%, with relative standard deviation values around 5.0%. The developed method is precise, sensitive, and reliable for analyzing Araucaria brown propolis.

    Topics: Abietanes; Araucaria; Brazil; Carboxylic Acids; Chemistry Techniques, Analytical; Chromatography, High Pressure Liquid; Diterpenes; Limit of Detection; Linear Models; Propolis; Reproducibility of Results; Tetrahydronaphthalenes

2021
Anti-mycobacterial natural products from the Canadian medicinal plant Juniperus communis.
    Journal of ethnopharmacology, 2012, Sep-28, Volume: 143, Issue:2

    Common juniper, Juniperus communis, is amongst the plants most frequently used by the indigenous peoples of North America for medicinal purposes. The First Nations of the Canadian Maritimes use infusions of juniper primarily as a tonic and for the treatment of tuberculosis. Previous investigations of extracts derived from the aerial parts of J. communis have shown it to possess anti-mycobacterial activity. The aim of the study is to isolate and identify anti-mycobacterial constituents from the aerial parts of J. communis.. Methanolic extracts of J. communis needles and branches were subjected to bioassay guided fractionation using the microplate resazurin assay (MRA) to assess inhibitory activity against Mycobacterium tuberculosis strain H37Ra. The anti-mycobacterial constituents were identified by NMR, MS and polarimetry.. The diterpenes isocupressic acid and communic acid and the aryltetralin lignan deoxypodophyllotoxin were isolated from the J. communis extract. Isocupressic acid and communic acid (isolated as an inseparable 3:2 mixture of cis and trans isomers) displayed MICs of 78 μM and 31 μM and IC(50)s of 46 μM and 15 μM against M. tuberculosis H37Ra respectively. Deoxypodophyllotoxin was less active, with a MIC of 1004 μM and an IC(50) of 287 μM.. Isocupressic acid, communic acid and deoxypodophyllotoxin were identified as the principal constituents responsible for the anti-mycobacterial activity of the aerial parts of J. communis. Although further research will be required to evaluate the relative activities of the two communic acid isomers, this work validates an ethnopharmacological use of this plant by Canadian First Nations and Native American communities.

    Topics: Anti-Bacterial Agents; Biological Products; Canada; Carboxylic Acids; Diterpenes; Drugs, Chinese Herbal; HEK293 Cells; Humans; Juniperus; Medicine, Traditional; Microbial Sensitivity Tests; Mycobacterium tuberculosis; Plant Components, Aerial; Plant Extracts; Plants, Medicinal; Podophyllotoxin; Tetrahydronaphthalenes

2012