colistin has been researched along with 1-(9-fluorenyl)methyl-chloroformate* in 1 studies
1 other study(ies) available for colistin and 1-(9-fluorenyl)methyl-chloroformate
| Article | Year |
|---|---|
A simple method for the assay of colistin in human plasma, using pre-column derivatization with 9-fluorenylmethyl chloroformate in solid-phase extraction cartridges and reversed-phase high-performance liquid chromatography.
A simple, selective and sensitive high-performance liquid chromatographic (HPLC) method is described for the determination of colistin in human plasma. Derivatization with 9-fluorenylmethyl chloroformate was performed in the same solid-phase extraction C18 cartridge used for sample pre-treatment, followed by reversed-phase HPLC with fluorimetric detection. Quantification was achieved using the ratio of the summed peak areas of colistin A and B derivatives to that of the derivative of netilmicin (internal standard). Linear calibration curves were obtained within the concentrations of colistin sulfate from 0.10 to 4.0 mg/l in plasma. Accuracy was within 10% and reproducibility (RSD) was less than 10%. Topics: Calibration; Chromatography, High Pressure Liquid; Colistin; Fluorenes; Fluorescent Dyes; Humans; Mass Spectrometry; Reproducibility of Results; Sensitivity and Specificity | 2001 |