coenzyme-q10 and quinone

coenzyme-q10 has been researched along with quinone* in 6 studies

Reviews

1 review(s) available for coenzyme-q10 and quinone

ArticleYear
The production of coenzyme Q10 in microorganisms.
    Sub-cellular biochemistry, 2012, Volume: 64

    Coenzyme Q10 has emerged as a valuable molecule for pharmaceutical and cosmetic applications. Therefore, research into producing and optimizing coenzyme Q10 via microbial fermentation is ongoing. There are two major paths being explored for maximizing production of this molecule to commercially advantageous levels. The first entails using microbes that naturally produce coenzyme Q10 as fermentation biocatalysts and optimizing the fermentation parameters in order to reach industrial levels of production. However, the natural coenzyme Q10-producing microbes tend to be intractable for industrial fermentation settings. The second path to coenzyme Q10 production being explored is to engineer Escherichia coli with the ability to biosynthesize this molecule in order to take advantage of its more favourable fermentation characteristics and the well-understood array of genetic tools available for this bacteria. Although many studies have attempted to over-produce coenzyme Q10 in E. coli through genetic engineering, production titres still remain below those of the natural coenzyme Q10-producing microorganisms. Current research is providing the knowledge needed to alleviate the bottlenecks involved in producing coenzyme Q10 from an E. coli strain platform and the fermentation parameters that could dramatically increase production titres from natural microbial producers. Synthesizing the lessons learned from both approaches may be the key towards a more cost-effective coenzyme Q10 industry.

    Topics: Agrobacterium tumefaciens; Benzoquinones; Escherichia coli; Fermentation; Genetic Engineering; Metabolic Networks and Pathways; Prokaryotic Cells; Ubiquinone

2012

Other Studies

5 other study(ies) available for coenzyme-q10 and quinone

ArticleYear
α-Tocotrienol quinone modulates oxidative stress response and the biochemistry of aging.
    Bioorganic & medicinal chemistry letters, 2011, Jun-15, Volume: 21, Issue:12

    We report that α-tocotrienol quinone (ATQ3) is a metabolite of α-tocotrienol, and that ATQ3 is a potent cellular protectant against oxidative stress and aging. ATQ3 is orally bioavailable, crosses the blood-brain barrier, and has demonstrated clinical response in inherited mitochondrial disease in open label studies. ATQ3 activity is dependent upon reversible 2e-redox-cycling. ATQ3 may represent a broader class of unappreciated dietary-derived phytomolecular redox motifs that digitally encode biochemical data using redox state as a means to sense and transfer information essential for cellular function.

    Topics: Aging; Animals; Antioxidants; Benzoquinones; Cells, Cultured; Dogs; Dose-Response Relationship, Drug; Humans; Mice; Molecular Structure; Oxidative Stress; Rats; Tocotrienols; Vitamin E

2011
Reaction of superoxide radical with quinone molecules.
    The journal of physical chemistry. A, 2011, Oct-27, Volume: 115, Issue:42

    When the superoxide radical O(2)(•-) is generated on reaction of KO(2) with water in dimethyl sulfoxide, the decay of the radical is dramatically accelerated by inclusion of quinones in the reaction mix. For quinones with no or short hydrophobic tails, the radical product is a semiquinone at much lower yield, likely indicating reduction of quinone by superoxide and loss of most of the semiquinone product by disproportionation. In the presence of ubiquinone-10, a different species (I) is generated, which has the EPR spectrum of superoxide radical. However, pulsed EPR shows spin interaction with protons in fully deuterated solvent, indicating close proximity to the ubinquinone-10. We discuss the nature of species I, and possible roles in the physiological reactions through which ubisemiquinone generates superoxide by reduction of O(2) through bypass reactions in electron transfer chains.

    Topics: Benzoquinones; Chemistry, Physical; Dimethyl Sulfoxide; Electron Spin Resonance Spectroscopy; Electron Transport; Oxidation-Reduction; Oxygen; Protons; Solutions; Superoxides; Ubiquinone

2011
Assay of coenzyme Q(10) in plasma by a single dilution step.
    Analytical biochemistry, 2002, Jun-01, Volume: 305, Issue:1

    A new method is described for determining coenzyme Q(10) (CoQ(10)) in plasma. The method is based on oxidation of CoQ(10) in the sample by treating it with para-benzoquinone followed by extraction with 1-propanol and direct injection into the HPLC apparatus. This method achieves a linear detector response for peak area measurements over the concentration range of 0.05-3.47 microM. Diode array analysis of the peak was consistent with CoQ(10) spectrum. Supplementation of the samples with known amounts of CoQ(10) yielded a quantitative recovery of 96-98.5%; the method showed a level of quantitation of 1.23 nmol per HPLC injection (200 microl of propanol extract containing 33.3 microl of plasma). A correlation of r = 0.99 (P < 0.0001) was found with a reference electrochemical detection method. Within run precision showed a CV% of 1.6 for samples approaching normal values (1.02 microM). Day-to-day precision was also close to 2%.

    Topics: Benzoquinones; Calibration; Centrifugation; Chromatography, High Pressure Liquid; Coenzymes; Electrochemistry; Enzyme Stability; Humans; Indicator Dilution Techniques; Linear Models; Oxidation-Reduction; Reproducibility of Results; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Ubiquinone

2002
Reduction of 1,4-quinone and ubiquinones by hydrogen atom transfer under UVA irradiation.
    Free radical research, 2002, Volume: 36, Issue:4

    1,4-Benzoquinone, coenzyme Q0 and Q10 were reacted with a series of hydrogen donors in the ESR cavity in the presence or absence of UVA irradiation. The signals of the radicals generated from the hydrogen donors or of those of the semiquinones were detected. The reaction mechanism was interpreted by a hydrogen atom transfer instead of the usual electron transfer mechanism on the basis of the redox potentials of the reactants and the Marcus theory. The hydrogen atom transfer is explained by the excited triplet state of quinones, which, on the basis of quantum mechanic calculations, may be reached even under visible light. In some cases, hydrogen atom transfer was also observed without irradiation, although to a lesser extent.

    Topics: Benzoquinones; Coenzymes; Electron Spin Resonance Spectroscopy; Free Radicals; Hydrogen; Hydrogen Bonding; Hydrogen Peroxide; Oxidation-Reduction; Protons; Thermodynamics; Ubiquinone; Ultraviolet Rays

2002
Stabilization of a semiquinone radical at the high-affinity quinone-binding site (QH) of the Escherichia coli bo-type ubiquinol oxidase.
    FEBS letters, 1995, Oct-30, Volume: 374, Issue:2

    Reaction of ubiquinone in the high-affinity quinone-binding site (QH) in bo-type ubiquinol oxidase from Escherichia coli was revealed by EPR and optical studies. In the QH site, ubiquinol was shown to be oxidized to ubisemiquinone and to ubiquinone, while no semiquinone signal was detected in the oxidase isolated from mutant cells that cannot synthesize ubiquinone. The QH site highly stabilized ubisemiquinone radical with a stability constant of 1-4 at pH 8.5 and the stability became lower at the lower pH. Midpoint potential of QH2/Q couple was -2 mV at pH 8.5 and showed -60 mV/pH dependence indicative of 2H+/2e- reaction. The Em was more negative than that of low-spin heme b above pH 7.0. We conclude that the QH mediates intramolecular electron transfer from ubiquinol in the low-affinity quinol oxidation site (QL) to low-spin heme b. Unique roles of the quinone-binding sites in the bacterial ubiquinol oxidase are discussed.

    Topics: Benzoquinones; Binding Sites; Coenzymes; Electron Spin Resonance Spectroscopy; Electron Transport; Electron Transport Complex IV; Enzyme Stability; Escherichia coli; Heme; Hydrogen-Ion Concentration; Oxidation-Reduction; Potentiometry; Spectrum Analysis; Ubiquinone

1995