clay and n-hexadecane

Red clay was previously used to enhance bioremediation of diesel-contaminated soil. It was speculated that the enhanced degradation of diesel was due to increased bacterial growth. In this study, we selected Acinetobacter oleivorans DR1, a soil-borne degrader of diesel and alkanes, as a model bacterium and performed transcriptional analysis using RNA sequencing to investigate the cellular response during hexadecane utilization and the mechanism by which red clay promotes hexadecane degradation. We confirmed that red clay promotes the growth of A. oleivorans DR1 on hexadecane, a major component of diesel, as a sole carbon source. Addition of red clay to hexadecane-utilizing DR1 cells highly upregulated β-oxidation, while genes related to alkane oxidation were highly expressed with and without red clay. Red clay also upregulated genes related to oxidative stress defense, such as superoxide dismutase, catalase, and glutaredoxin genes, suggesting that red clay supports the response of DR1 cells to oxidative stress generated during hexadecane utilization. Increased membrane fluidity in the presence of red clay was confirmed by fatty acid methyl ester analysis at different growth phases, suggesting that enhanced growth on hexadecane could be due to increased uptake of hexadecane coupled with upregulation of downstream metabolism and oxidative stress defense. The monitoring of the bacterial community in soil with red clay for a year revealed that red clay stabilized the community structure.

Topics: Acinetobacter; Alkanes; Aluminum Silicates; Biodegradation, Environmental; Biota; Carbon; Clay; Oxidative Stress; RNA, Ribosomal, 16S; Sequence Analysis, RNA; Soil Microbiology; Soil Pollutants

We evaluated the role of soil aggregate pore size on biodegradation of essentially insoluble petroleum hydrocarbons that are biodegraded primarily at the oil-water interface. The size and spatial distribution of pores in aggregates sampled from biodegradation experiments of a clayey, aggregated, hydrocarbon-contaminated soil with relatively high bioremediation end point were characterized by image analyses of X-ray micro-CT scans and N2 adsorption. To determine the bioaccessible pore sizes, we performed separate experiments to assess the ability of hydrocarbon degrading bacteria isolated from the soil to pass through membranes with specific sized pores and to access hexadecane (model insoluble hydrocarbon). Hexadecane biodegradation occurred only when pores were 5 μm or larger, and did not occur when pores were 3 μm and smaller. In clayey aggregates, ∼ 25% of the aggregate volume was attributed to pores larger than 4 μm, which was comparable to that in aggregates from a sandy, hydrocarbon-contaminated soil (~23%) scanned for comparison. The ratio of volumes of inaccessible pores (<4 μm) to bioaccessible pores (>4 μm) in the clayey aggregates was 0.32, whereas in the sandy aggregates it was approximately 10 times lower. The role of soil microstructure on attainable bioremediation end points could be qualitatively assessed in various soils by the aggregate characterization approach outlined herein.

Topics: Alkanes; Aluminum Silicates; Bacteria; Biodegradation, Environmental; Bioreactors; Clay; Hydrocarbons; Minerals; Molecular Weight; Particle Size; Petroleum; Porosity; Soil; Soil Microbiology; Soil Pollutants; Time Factors; X-Ray Microtomography

Clayey soils contaminated with organic pollutants are nowadays one of the important environmental issues as they are highly reluctant to conventional bioremediation techniques. In this study, biodegradability of n-hexadecane as a model contaminant in oil polluted clayey soil by an indigenous bacterium was investigated. Maximal bacterial growth was achieved at 8% (v/v) n-hexadecane as sole carbon and energy sources in aqueous phase. The predominant n-hexadecane uptake mechanism was identified to be biosurfactant-mediated using bacterial adhesion to hydrocarbon (BATH) test and surface tension measurements. The effect of n-hexadecane concentration, soil to water ratio, inoculum concentration and pH on total organic carbon (TOC) reduction from kaolin soil in slurry phase was investigated at two levels in shake flasks using full factorial experimental design method where 10,000 (mg n-hexadecane)(kg soil)(-1), soil-water ratio of 1:3, 10% (v/w) inoculum and pH of 7 resulted in the highest TOC reduction of 70% within 20 days. Additionally, slurry bioreactor experiments were performed to study the effect of various aeration rates on n-hexadecane biodegradation during 9 days where 2.5 vvm was found as an appropriate aeration rate leading to 54% TOC reduction. Slurry phase bioremediation is shown to be a successful method for remediation of clayey reluctant soils.

Topics: Alkanes; Aluminum Silicates; Bacteria; Biodegradation, Environmental; Bioreactors; Carbon; Clay; Soil Microbiology; Soil Pollutants

Topics: Adsorption; Alkanes; Aluminum Silicates; Clay; Nitro Compounds; Soil; Soil Pollutants; Water; Water Pollutants, Chemical

Sorption of nitrobenzene, phenol, and m-nitrophenol from water and n-hexadecane was measured on Na-montmorillonite and organoclays in which 41 and 90% of the exchange capacity of the Na-clay was occupied by hexadecyltrimethylammonium. The strength of sorbate-sorbent interactions in n-hexadecane for all three sorbents was in the following order: nitrobenzene < phenol < m-nitrophenol. The magnitude of the distribution coefficients suggests that the contribution to solute uptake of partitioning between n-hexadecane and the organic pseudophase of the dried organoclays is minor, whereas the major contribution is from adsorptive sorbate-sorbent interactions. Sorption isotherms obtained in different solvents were compared using a sorbate activity scale. In the organoclays, the stronger the tendency of a sorbate to interact with sorption sites, the less pronounced is the reduction in the activity-based sorption due to competition with water. The order of this reduction for the different sorbates is nitrobenzene > phenol > m-nitrophenol. The weakening of sorbate-sorbent interactions resulting from water-sorbate competition might be mitigated by interaction between the organic sorbate and sorbed water molecules. Since the more strongly interacting organic compounds are less susceptible to suppression of sorption in the presence of water, hydrating organoclays may result in an increased differentiation between "weakly" and "strongly" interacting ("nonpolar" and "polar") compounds in the organoclay phase.

Topics: Adsorption; Alkanes; Aluminum Silicates; Clay; Hydrophobic and Hydrophilic Interactions; Organic Chemicals; Water