citreoviridin and mutilin

Fungal natural products (NPs) comprise a vast number of bioactive molecules with diverse activities, and among them are many important drugs. However, the yields of fungal NPs from native producers are usually low, and total synthesis of structurally complex NPs is challenging. As such, downstream derivatization and optimization of lead fungal NPs can be impeded by the high cost of obtaining sufficient starting material. In recent years, reconstitution of NP biosynthetic pathways in heterologous hosts has become an attractive alternative approach to produce complex NPs. Here, we present an efficient, cloning-free strategy for the cluster refactoring and total biosynthesis of fungal NPs in

Topics: Aspergillus nidulans; Aurovertins; Benzofurans; Biological Products; Biosynthetic Pathways; Diterpenes; Genes, Fungal; Homologous Recombination; Ketones; Multigene Family; Plasmids; Pleuromutilins; Polycyclic Compounds; Regulon