chrysoeriol and kaempferol

Micro-liquid chromatography (microLC) in conjunction with multistage mass spectrometry (MSn) was introduced to study several major heartsease flavonoid glycosides. High-resolution microLC separation was achieved by using a monolithic poly(p-methylstyrene-co-1,2-bis(p-vinylphenyl)ethane) column under reversed-phase conditions. The MS/MS and MS3 analysis of the flavonoid components of interest provided data about their glycosylation type and position, nature of their aglycones, and the structure/linkage information of their glycan moieties. With our microLC-MSn approach, four flavonol O-glycosides, nine flavone-C-glycosides, and three flavone C,O-glycosides were characterized in heartsease methanol extract. All of these glycoconjugates were found to be the derivatives of six aglycones: apigenin, chrysoeriol, isorhamnetin, kaempferol, luteolin, and quercetin.

Topics: Apigenin; Chromatography, High Pressure Liquid; Chromatography, Liquid; Flavones; Flavonoids; Flavonols; Glycosides; Kaempferols; Luteolin; Mass Spectrometry; Quercetin; Spectrometry, Mass, Electrospray Ionization; Viola

To study the chemical constituents of Myricaria bracteata.. The chemical constituents were isolated by silica gel column chromatography and the structures were elucidated by spectroscopic methods.. Eleven compounds were obtained and identified as rhamnetin, 3,5,4'-trihydroxy-7,3'-dimethoxyflavone, 3,5,4'-trihydroxy-7-methoxyflavone, quercetin-3-O-alpha-L-rhamnopyranoside, kaempferol, quercetin, chrysoerio, gallic acid, gallic acid ethylester, beta-sitosterol, daucosterol.. All compounds were obtained from M. bracteata for the first time.

Topics: Flavones; Flavonoids; Glycosides; Kaempferols; Monosaccharides; Plants, Medicinal; Quercetin; Tamaricaceae