chrome-azurol-s and ascamycin

A new siderophore glucuronide, nocardamin glucuronide (1), was isolated together with nocardamin (2) by applying the one strain-many compounds (OSMAC) approach to the ascamycin-producing strain, Streptomyces sp. 80H647, and performing multivariate analysis using mass spectral data. Structure elucidation was accomplished by a combination of NMR and MS analyses. The absolute configuration of the glucuronic acid moiety was found to be β-D-GlcA by hydrolysis using β-glucuronidase, subsequent derivatization of the hydrolysate, and comparison with standards. The siderophore activity of 1 was evaluated through the chrome azurol S assay and was comparable to that of 2 and deferoxamine (IC

Topics: Adenosine; Anti-Infective Agents; Antimalarials; Cell Line, Tumor; Deferoxamine; Humans; Hydroxybenzoates; Iron; Magnetic Resonance Spectroscopy; Mass Spectrometry; Molecular Structure; Peptides, Cyclic; Principal Component Analysis; Siderophores; Streptomyces; Toxicity Tests