chondroitin-sulfates and thiazolyl-blue

chondroitin-sulfates has been researched along with thiazolyl-blue* in 3 studies

Other Studies

3 other study(ies) available for chondroitin-sulfates and thiazolyl-blue

ArticleYear
Storage correction in cells of patients suffering from mucopolysaccharidoses types IIIA and VII after treatment with genistein and other isoflavones.
    Journal of inherited metabolic disease, 2010, Volume: 33, Issue:1

    Mucopolysaccharidoses are autosomal and recessive lysosomal storage disorders caused by the deficiency of a lysosomal enzyme involved in glycosaminoglycan catabolism. The Sanfilippo type A disease (MPS III A) results from sulfamidase deficiency, which leads to accumulation of heparan sulfate, whereas Sly disease (MPS VII) results from beta-glucuronidase deficiency, leading to accumulation of heparan, dermatan, and chondroitin sulfates. These syndromes are characterized by severe central nervous system degeneration, resulting in progressive mental retardation, and fatality occurs in severely affected children. To date, no effective treatment is available except for bone marrow transplantation in specific cases. Recently, the use of genistein, an isoflavone that inhibits glycosaminoglycans synthesis, has been tested as substrate reduction therapy for neuronopathic forms of these diseases.We tested five natural analogs to genistein in human fibroblasts from both Sanfilippo A and Sly patients. Four molecules were as efficient as genistein in decreasing glycosaminoglycan accumulation. Moreover, a combination of several isoflavones was more efficient than one single isoflavone, suggesting a synergistic effect. These preliminary data may offer new perspectives for treating Sly and Sanfilippo A diseases and could be relevant to other neurological forms of mucopolysaccharidoses.

    Topics: Bone Marrow Transplantation; Chondroitin Sulfates; Dose-Response Relationship, Drug; Fibroblasts; Genistein; Glycosaminoglycans; Humans; Isoflavones; Lysosomes; Models, Biological; Models, Chemical; Mucopolysaccharidosis III; Mucopolysaccharidosis VII; Tetrazolium Salts; Thiazoles

2010
Polyamide 6 composite membranes: properties and in vitro biocompatibility evaluation.
    Journal of biomaterials science. Polymer edition, 2001, Volume: 12, Issue:1

    The aim of the present study was to develop polyamide 6 membrane blended with gelatin and chondroitin sulfate using the phase precipitation method and evaluate its in vitro biocompatibility. Morphology of membranes was studied by laser scanning confocal microscopy which allowed the nondestructive visualization of internal bulk morphology of membranes. Membranes exhibited porous morphology with pores spanning across the membrane width with interconnections at various depths. Membranes showed adequate mechanical properties with tensile strengths of 20.10 +/- 0.64 MPa, % strain of 3.01+/-0.07, and modulus of 1082.50+/-23.50 MPa. In vitro biocompatibility of membranes by direct contact test did not show degenerative effects on NIH3T3 cells and also its leach-out products (LOP), as determined by tetrazolium (MTT) and neutral red uptake (NRU) assay. Mouse peritoneal macrophage cultured in contact with membranes and PTFE control showed comparable expression of activation markers such as CD11b/CD18, CD45, CD14, and CD86 suggesting the membranes' non-activating nature. Membrane LOP did not induce excessive proliferation of mouse splenocytes suggesting its non-antigenic nature. Preliminary blood compatibility of membranes was observed with no detectable hemolysis in static incubation assay. Taken collectively, the present data demonstrate that polyamide 6 composite membranes are biocompatible and prospective candidates for tissue engineering applications.

    Topics: 3T3 Cells; Animals; Antigens, CD; B7-2 Antigen; Biocompatible Materials; Caprolactam; Cell Division; Cell Membrane; Cell Separation; Chondroitin Sulfates; Coloring Agents; DNA Damage; Flow Cytometry; Gelatin; Humans; Leukocyte Common Antigens; Lipopolysaccharide Receptors; Lymphocytes; Macrophage-1 Antigen; Membrane Glycoproteins; Membranes, Artificial; Mice; Mice, Inbred BALB C; Microscopy, Confocal; Neutral Red; Polymers; Spleen; Tetrazolium Salts; Thiazoles

2001
Assessment of cornea viability by confocal laser scanning microscopy and MTT assay.
    Cornea, 1997, Volume: 16, Issue:6

    Determination of excised cornea viability is of interest for transplant-storage evaluation, but also for in vitro diffusion-study design and ocular-toxicity assessment. By using simultaneous vital staining by calcein AM (CAM) and ethidium homodimer-1 (EH-1), as "live" and "dead" probes, respectively, we developed a confocal laser scanning microscopy (CLSM) assay to determine epithelial and endothelial viability and estimate cornea thickness.. New Zealand White rabbit corneas were stored in phosphate-buffered saline (PBS) or Optisol at 4 degrees C or at room temperature. At various times, corneas were stained with an EH-1/CAM solution and observed, without further treatment, by CLSM. Storage effects on the cornea were also assessed by using an MTT assay.. Stromal swelling, shedding of the upper epithelial layers, and severe endothelial damage were observed after 4 h in PBS at room temperature. After 8 h, lower epithelial cell death was observed, along with loss of endothelial structure. Corneas stored in similar conditions in Optisol were indistinguishable from controls. Storage in Optisol at 4 degrees C affected the superficial layers of the corneal epithelium similarly at both 7 and 14 days. Extensive epithelial shedding and wing-cell death were observed at 25 days, but the basal layer remained approximately 50% healthy. Significant endothelial cell loss was observed at 25 days. MTT results were consistent with CLSM data in the medium-term storage study only.. This CAM/EH-1 CLSM fluorescence assay is a sensitive index of viability in cornea, and thus may prove useful in investigations in which maintenance of vital functions in different cell layers is critical.

    Topics: Animals; Biological Assay; Cell Survival; Chondroitin Sulfates; Coloring Agents; Complex Mixtures; Cornea; Corneal Transplantation; Culture Media, Serum-Free; Dextrans; Ethidium; Female; Fluoresceins; Fluorescent Dyes; Gentamicins; Intercalating Agents; Male; Microscopy, Confocal; Microscopy, Fluorescence; Rabbits; Tetrazolium Salts; Thiazoles; Tissue Preservation

1997
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