chondroitin-sulfates has been researched along with 2-cyanoacetamide* in 1 studies
1 other study(ies) available for chondroitin-sulfates and 2-cyanoacetamide
| Article | Year |
|---|---|
Microdetermination of hyaluronan in human plasma by high-performance liquid chromatography with a graphitized carbon column and postcolumn fluorometric detection.
A chemical method for the determination of hyaluronan (hyaluronic acid, HA) has been developed and applied to the human blood plasma. Human blood plasma HA was converted to the ΔDi-HA by digestion with hyaluronidase SD and determined by a sensitive and selective high-performance liquid chromatography (HPLC). The HPLC includes the separation and detection of ΔDi-HA using a graphitized carbon column and fluorometric reaction with 2-cyanoacetamide in an alkaline eluent. The calibration graph for ΔDi-HA was linear over the range 0.2 ng-1 μg. It was revealed that the concentration of HA in normal human blood plasma is very low levels (about 24 ng/ml) in comparison to low-sulfated chondroitin 4-sulfate (about 13 μg/ml). Topics: Adult; Chondroitin Sulfates; Chondroitinases and Chondroitin Lyases; Chromatography, High Pressure Liquid; Female; Fluorometry; Graphite; Humans; Hyaluronic Acid; Hyaluronoglucosaminidase; Male; Nitriles; Sensitivity and Specificity | 2011 |