chondroitin and thiobarbituric-acid

chondroitin has been researched along with thiobarbituric-acid* in 1 studies

Other Studies

1 other study(ies) available for chondroitin and thiobarbituric-acid

ArticleYear
Hydroxyl radical scavenging activity of a new ophthalmic viscosurgical device.
    Current eye research, 2007, Volume: 32, Issue:2

    To quantify the hydroxyl radical scavenging activity of a new ophthalmic viscosurgical device (OVD) based on sodium hyaluronate and hydroxypropylmethylcellulose (named VISC28) in comparison with Viscoat, Healon, and Amvisc Plus.. The hydroxyl radicals that represent the principal free-radical species generated during phacoemulsification were produced by the Fenton reaction, and the scavenging activity of the tested viscoelastic substances was evaluated in vitro by the 2-deoxy-D-ribose (2-DR) oxidation method that produces the thiobarbituric acid-malondialdehyde (TBA-MDA), complex. An aliquot of viscosurgical formulation was added to phosphate buffer and mixed with 2-DR, Fe2 +/ethylenediaminetetraacetic acid (EDTA), and H2O2. The sample mix was incubated and thiobarbituric acid-trichloroacetic acid solution was added. The sample was then incubated for 30 min, and a chromatographic analysis was performed to quantify the TBA-MDA complex. The data were expressed as micromoles of MDA per milliliter of sample.. All tested OVDs showed a marked hydroxyl radical scavenging activity. The MDA level was significantly lower in VISC28 (0.045 +/- 0.007 micromol/ml) compared with Viscoat (0.070 +/- 0.012 micromol/ ml, p < 0.05), Amvisc Plus (0.111 +/- 0.008 micromol/ml, p < 0.001), and Healon (0.175 +/- 0.016 micromol/ml, p < 0.001). A reduced scavenging activity was shown by VISC28 phosphate-buffered solution (PBS) (no TRIS and no sorbitol) compared with VISC28 (p < 0.001).. The new OVD, VISC28, showed significantly higher hydroxyl radical inhibition compared with the other viscosurgical formulations. The following rank order for the scavenging activity was established: VISC28 > Viscoat > Amvisc Plus > Healon.

    Topics: Chondroitin; Chondroitin Sulfates; Deoxyribose; Drug Combinations; Free Radical Scavengers; Hyaluronic Acid; Hydrogen Peroxide; Hydroxyl Radical; Hypromellose Derivatives; Iron; Malondialdehyde; Methylcellulose; Ophthalmologic Surgical Procedures; Oxidation-Reduction; Sorbitol; Thiobarbiturates; Tromethamine

2007
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